CN101215539A - Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof - Google Patents
Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof Download PDFInfo
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Abstract
The invention relates to a microbe germ composition, a cyanophyte biological germ leaven which contains the microbe germ, a cyanophyte fermentation nutrient, a cyanophyte biological organic fertilizer and a method for preparation, wherein the microbe germ composition contains plant lactobacillus, bacillus subtilis and swamp Rhodopseudomonas, the cyanophyte biological germ leaven is prepared by microbe germ composition and base, the cyanophyte fermentation nutrient is prepared by cyanophyte biological germ leaven and cyanophyte, the cyanophyte biological organic fertilizer is prepared by cyanophyte fermentation nutrient, organics base and water-loss reducer. The microbe germ composition is provided with the ability for fermenting solid cyanophyte, which can transform nutriment in the cyanophyte into the material for crops. The cyanophyte fermentation nutrient can be superior animal feed. The cyanophyte biological organic fertilizer is provided with crop pest control effect, which adds the soil organics content and improves nutrient utility to accelerate the plant growth belonging to natural superior organics fertilizer.
Description
Technical field
The present invention relates to a kind of microbial bacteria composition, contain biological bacteria starter, blue algae fermentation nutrition and blue-green algae biological organic fertilizer of this microbial bacteria composition and preparation method thereof.In more detail, the present invention relates to a kind of ferment with the microbial bacteria composition and contain this fermentation with the blue-green algae biological bacteria starter of microbial bacteria composition, contain the blue algae fermentation nutrition of this blue-green algae biological bacteria starter and contain nutraceutical blue-green algae biological organic fertilizer of this blue algae fermentation and preparation method thereof.
Background technology
Along with industrialization develops rapidly, use high phosphorated washing composition and the chemical fertilizer that is rich in phosphorus, nitrogen in a large number; Undressed people, fowl, animal manure and sanitary sewage, trade effluent etc. are entered the waters, cause rivers and lakes to lose the ecological functions of original selfreparing, self-purification, the eutrophication degree aggravation of water body.Since blue-green algae (Cynophyta) like substrate be mud and be rich in nitrogen phosphorus and organic shallow lake in grow, when reaching more than 15 ℃, water temperature just begins procreation, high more its growth procreation speed of water temperature is also just fast more, therefore, when every year, early autumn in midsummer, the lake water temperature reached more than 20 ℃, in also its colony highly expands just.The generation of blue-green algae has not only seriously hindered other planktonic normal growth, and can be in the waters of its covering, the entering of block sunlight and oxygen; After blue-green algae death was rotted, under anaerobic environment, release had malodorous hydrogen sulfide, methane and algae toxin etc. in a large number again, further caused the fishes and shrimps shellfish in the water body to cause a disease dead because of the anoxic poisoning; Thereby make river, lake water quality deterioration, contaminate environment.Blue-green algae has caused the extensive concern of the world today to the pollution of water body in lake, and at home, this problem is particularly urgent.Blue-green algae is active in all kinds of freshwater lake water bodys, have very strong vitality, its a large amount of breedings will seriously influence the quality of water body, cause serious water to pollute, the shortage of Freshwater resources and pollution have become one of principal element of harm humans health in the world.The blue-green algae of therefore administering water resources pollutes and becomes global problem.If meanwhile the blue-green algae in the blue-green algae governance process can not get good utilization, also will cause secondary pollution.
Except that objectionable impurities, also have a large amount of eutrophy compositions in the blue-green algae.Organic matter reaches more than 65.% in the natural blue-green algae, in the blue-green algae body nitrogen content up to about 10%, phosphorus content also nearly 2%.The protein content of algae mud reaches 40%, and is suitable with the protein content of soybean, has rich in amino acid and VITAMIN simultaneously, and nutritive ingredient is high.Therefore when administering the pollution of water resources blue-green algae, must consider the problem of the comprehensive utilization of blue-green algae, by the processing of science, blue-green algae can become very excellent pure natural organic bio-fertilizer and high-quality feed fully.
Salvaging is to administer the most direct effective means that the water body blue-green algae pollutes at present.The blue-green algae that is salvaged can not be directly used in fertilizer and feed, because of fresh blue-green algae has one deck colloid coat, effectively nutritive ingredient can not directly be digested by plant utilization and animal, must handle through dehydration and drying when making fertilizer and feed, is broken into powdery and processes and obtain through refining.The commercialization of blue-green algae at present also is faced with obstacles such as industrial production cost height, dewatered drying power consumption be big.Therefore, a large amount of blue-green algaes of salvaging are discarded on the water body in lake bank base and can not get effective utilization, cause secondary pollution and pollution transportation.
For overcoming the defective that natural blue-green algae exists, the invention provides a kind of fermentation microbial bacteria composition, described fermentation with the microbial bacteria composition in antagonism not between each microorganism live bacteria, work in coordination with mutually; And described fermentation can be used for preparing blue-green algae biological bacteria starter, blue algae fermentation nutrition and blue-green algae biological organic fertilizer with the microbial bacteria composition.
Summary of the invention
Primary and foremost purpose of the present invention is to provide a kind of fermentation microbial bacteria composition;
Another object of the present invention is to provide a kind of blue-green algae biological bacteria starter that contains above-mentioned fermentation with the microbial bacteria composition;
Another object of the present invention is to provide a kind of blue algae fermentation nutrition that contains above-mentioned blue-green algae biological bacteria starter;
Another object of the present invention is to provide a kind of nutraceutical blue-green algae biological organic fertilizer of above-mentioned blue algae fermentation that contains;
Another purpose of the present invention is to provide a kind of preparation method of blue-green algae biological bacteria starter;
Another purpose of the present invention is to provide a kind of blue algae fermentation nutraceutical preparation method;
Further purpose of the present invention is to provide a kind of preparation method of blue-green algae biological organic fertilizer.
The present invention relates to a kind of fermentation microbial bacteria composition, it contains the bacterial classification of following parts by weight proportioning:
10～40 parts of plant lactobacilluss,
5～20 parts of subtilises,
5～20 parts of Bacillus licheniformis,
30～60 parts of Rhodopseudomonas palustriss.
According to fermentation of the present invention microbial bacteria composition, the parts by weight proportion optimization of wherein said each bacterial classification is:
20～35 parts of plant lactobacilluss,
10～20 parts of subtilises,
10～15 parts of Bacillus licheniformis,
30～60 parts of Rhodopseudomonas palustriss.
According to the blue-green algae biological bacteria starter that contains above-mentioned fermentation with the microbial bacteria composition of the present invention, its each component by following parts by weight proportioning is prepared from:
10～30 parts of microbial bacteria compositions of fermentation,
50～110 parts of base-materials.
According to blue-green algae biological bacteria starter of the present invention, wherein said base-material is selected from the mixture of the peat composed of rotten mosses and stalk, and its mixed weight umber proportioning is:
10～30 parts of the peats composed of rotten mosses,
40～80 parts of stalks.
According to the blue algae fermentation nutrition that contains above-mentioned blue-green algae biological bacteria starter of the present invention, its each component by following parts by weight proportioning is prepared from:
10～40 parts of blue-green algae biological bacteria starters,
60～90 parts of blue-green algaes.
According to the nutraceutical blue-green algae biological organic fertilizer of above-mentioned blue algae fermentation that contains of the present invention, its each component by following parts by weight proportioning is prepared from:
55～85 parts of blue algae fermentation nutrition,
11～40 parts of organism base-materials,
1～10 part of water-holding agent.
According to blue-green algae biological organic fertilizer of the present invention, wherein said organism base-material is oligochitosan, brown coal, rich potassium shale and bentonitic mixture, and its parts by weight proportioning is:
0.5～10 part of oligochitosan,
10～30 parts in brown coal,
40～80 parts in rich potassium shale,
1～10 part of wilkinite.
According to blue-green algae biological organic fertilizer of the present invention, wherein said water-holding agent is selected from one or more in ammonium potassium agglutinent water-loss reducer of soil, non-metallic minerals water-loss reducer of soil, polyacrylamide water-holding agent, polyacrylate and the polyacrylamide interpolymer water-loss reducer of soil.
The present invention relates to the preparation method of above-mentioned blue-green algae biological bacteria starter, it may further comprise the steps:
(1) base-material of 50～110 parts of weight is made powder, the high-temperature sterilization postcooling;
(2) the microbial bacteria composition of 10～30 parts of weight of adding stirs and makes blue-green algae biological bacteria starter, and total water content is 25%.
The invention still further relates to the nutraceutical preparation method of above-mentioned blue algae fermentation, it may further comprise the steps:
(1) initial water content of adjustment blue-green algae is removed foreign material;
(2) the blue-green algae biological bacteria starter with 10～40 parts of weight adds in the blue-green algae of 60～90 parts of weight; With
(3) stir, and the temperature bottom fermentation between 35 ℃～65 ℃, oven dry.
According to the nutraceutical preparation method of blue algae fermentation of the present invention, wherein the initial water content with blue-green algae is controlled at 70%～80%.
According to the nutraceutical preparation method of blue algae fermentation of the present invention, wherein Fa Jiao time is 96～240 hours, is preferably 120～192 hours.
According to the nutraceutical preparation method of blue algae fermentation of the present invention, wherein the stirring time during the fermentation is 4～16 hours, is preferably 8～12 hours.
The present invention relates to the preparation method of above-mentioned organism base-material in addition, and it may further comprise the steps:
(1) oligochitosan, brown coal, rich potassium shale and wilkinite are made powder respectively, the high-temperature sterilization postcooling;
(2) oligochitosan, the brown coal of 10～30 parts of weight, the rich potassium shale of 40～80 parts of weight and the wilkinite of 1～10 part of weight with 0.5～10 part of weight of above-mentioned preparation mixes, and stirs and makes the organism base-material.
The present invention relates to the preparation method of above-mentioned blue-green algae biological organic fertilizer in addition, and it may further comprise the steps:
(1) the organism base-material of 11～40 parts of weight is pulverized high-temperature sterilization, cooling;
(2) the blue algae fermentation nutrition with 55～85 parts of weight adds in the described organism base-material, stirs, dries; With
(3) water-holding agent that adds 1～10 part of weight mixes stirring again, adopts the preparation technology who produces particle fertilizer to make described blue-green algae biological organic fertilizer.
According to the preparation method of blue-green algae biological organic fertilizer of the present invention, the preparation technology of wherein said particle fertilizer is selected from extrusion process, disk comminution granulation, drum granulating method, screw rod extruding pelletization method, spherolite molding, granulating method, pair roller non-slurry pelletizing method and to the tooth comminution granulation.
In addition, the present invention also relates to the blue algae fermentation nutrition and be used alone or in combination purposes as animal-feed and bait.
In addition, the invention still further relates to the blue-green algae biological organic fertilizer can be used alone or in combination the fertilizer of growing seedlings, the base manure that can be used as farm crop and plant, topdress and foliage fertilizer.
The invention still further relates to the using method of described blue-green algae biological organic fertilizer, it comprises:
(1) fertilizer of growing seedlings applies 2～3 kilograms of blue-green algae biological organic fertilizers and is used for crop, vegetables, plant etc. and grows seedlings by per 10 square meters bed of growing seedlings;
(2) base manure imposes in the soil before the field planting such as crop, vegetables, plant by every mu of 80～100 kilograms of blue-green algae biological organic fertilizers; With
(3) topdress, dash as topdressing of crop, vegetables, plant etc. by every mu of 20～30 kilograms of blue-green algae biological organic fertilizers and execute.
The microbial bacteria involved in the present invention and the explanation of other raw material:
One, microbial bacteria
(1) plant lactobacillus (Lactobacillus plantarum)
(2) subtilis (Bacillus Subtilis)
(3) Bacillus licheniformis (Bacillus lichenifdrmis)
(4) swamp Rhodopseudomonas (Rhodosedoserdomouas padustris)
Above microbial strains is in Chinese agriculture microbial strains preservation administrative center, and deposit number is respectively: 1,11016; 2,11062; 3,11080; 4,10649;
The microorganism that the present invention uses both can have been used contained microorganism in the live bacteria agent sold on the market etc., the microorganism that also can use the strain culturing of selling on the market.
Two, oligochitosan
Oligochitosan (plant amino-oligosaccharide) is an ocean bottom sediments shells extract matter, itself contains abundant organic carbon (C) and organonitrogen (N), can be utilized by microbiological degradation and as the nutrient of plant-growth; Can change soil microflora, the promotion beneficial microorganism is grown and is suppressed some phytopathogens; But the disease resistance of inducing plant produces immunity and killing action to multiple fungi, bacterium and virus.
The present invention contains the blue-green algae biological organic fertilizer of oligochitosan, can effectively promote crop growth, and crop virosis such as cucumber mosaic virus are had tangible preventive and therapeutic effect.Organic carbon (C) content 〉=40% of oligochitosan (plant amino-oligosaccharide), organonitrogen (N) content 〉=8%.
Three, the combination of organic substance and nutritive element
(1) natural blue-green algae: organic 〉=65% in the natural blue-green algae (Cynophyta), nitrogen content in the blue-green algae 〉=10%, phosphorus content 〉=1%.The protein content of algae mud 〉=40%, suitable with the protein content of soybean, have rich in amino acid and vitamins and other nutritious components simultaneously.
(2) brown coal: be natural coal slime material, the ature of coal trade mark is brown coal, is microorganism high-quality sorbent material.Total humic acid 〉=30%, free humic acid 〉=30%, organic (air dried basis) 〉=80%, ash 10～15%, xanthohumic acid 〉=10%, calcium 〉=10%, magnesium 〉=1%, trace elements such as iron content, manganese, silicon.PH value 6～7.
(3) peat composed of rotten mosses: be natural herb (woody) charcoal mud material, organic carbon (C) content height is the good nutrition sorbent material of microorganism.Full nitrogen 〉=2%, full phosphorus 〉=0.2%, full potassium 〉=0.1%, organic 〉=80%, free humic acid 〉=45%, crude ash content 10～15%, organic carbon 30～40%, pH value 5.5～6.
(4) rich potassium shale: the rich potassium shale of natural mineral, ore contains potassium (K based on black and green illite shale
2O) 10～15%, silicon (SiO
2) 30～40%, magnesium (MgO) 0.8～1.1%, iron (TFe) 4～4.5%.
(5) wilkinite: the wilkinite that the present invention selects for use is as the fertile tackiness agent of particle.This product is picked up from natural montmorillonite ore deposit, and product is a white powder, and is nontoxic, tasteless, smectite content 95-98% wherein, silicon-dioxide (SiO
2) content 3～5%, aluminium sesquioxide (Al
2O
3) 0.5～1%.
(6) stalk: the farm crop body of rod, mainly use wheat straw, corn stalk, straw etc.Carbon containing 〉=45%, nitrogenous 〉=0.5%.
(7) water-holding agent: selected water-holding agent is an ammonium potassium agglutinent water-loss reducer of soil in the specific embodiments of the invention, does not wherein contain the deleterious sodium of soil, and this product can be decomposed into carbonic acid gas, water and nitrogen under the effect of sunlight, useful and harmless to soil.
The water-holding agent that the present invention uses can also be non-metallic minerals water-loss reducer of soil, polyacrylamide water-holding agent or polyacrylate and polyacrylamide interpolymer water-loss reducer of soil, but is not limited only to above-mentioned several.
Wherein, the non-metallic minerals water-loss reducer of soil can be compound with non-metallic minerals and macromolecular material, and having improved product salt tolerant alkalescence can, improved the adaptability of product in soil, be applied to soil after, can not produce pollution, on the other hand, its adding can reduce the content of polymer moiety.Realize natural degradation under the microorganism that polymer moiety can be in soil and the acting in conjunction of Mycophyta, noresidue can not produce any negative impact to soil, plant and environment for human survival.
The polyacrylamide water-holding agent then is to form hydrogel at plant root soil and suction on every side thereof, root system can in time obtain moisture nutrition, the water holding capacity of growth matrix improves greatly like this, thereby promotes plant-growth, and the reduction of soil water flow vector simultaneously also makes water consumption and the number of times that waters reduces.Because water-holding agent can absorb and discharge moisture in a large number in soil, first imbibition post shrinkage, this physical action repeatedly makes a kind of air permeability and good of Soil conservation promote the plant root growth.
Polyacrylate and polyacrylamide interpolymer water-loss reducer of soil have nontoxic, safety and environmental protection, and be nonpoisonous and tasteless, do not pollute plant, soil and underground water, and final resolvent is carbonic acid gas, water, ammonia-state nitrogen and sodium or potassium ion, does not have any residual.
From improving crop disease-resistant and improving the viewpoint of making produce matter and consider, can randomly comprise microorganism, water-holding agent and base-material in the above-mentioned scope according to cropping region soil and crop varieties situation, wherein said base-material is oligochitosan (plant amino-oligosaccharide), blue-green algae, brown coal, the peat composed of rotten mosses, rich potassium shale, wilkinite etc. for example, just can obtain the blue-green algae biological organic fertilizer of good combination property of the present invention.
Nutraceutical experimental data result is as follows for blue algae fermentation of the present invention:
I. the blue algae fermentation nutrition is used to the test of feeding flounder as feed
1. test conditions and method
1. basal feed: basal feed adopts sea water fish powdery mixed feed.Its main component is fish meal, dregs of beans, peanut meal, wheat bran etc.
2. control group: mix with the basal feed of 300kg and the iced trash fish of 300kg before feeding, make soft particle with electronic nodulizer and organize feed in contrast, common 600kg.
3. test group: mix with the blue algae fermentation nutrition A of the following embodiment 10 of 300kg and the iced trash fish of 300kg before feeding, make soft particle as the test group feed, common 600kg with electronic nodulizer.
4. experimental animal: 85g～120g flounder kind is healthy anosis.
5. test design: adopt single-factor design at random, establish control group and test group.Other condition is consistent between each group.Every productive capacity with control group is a reference value, the nutraceutical result of use of Comprehensive Assessment blue algae fermentation.
6. fish-culture test design: every kind of feed is a treatment group.Handle 2 repetitions for every group, each repeating groups is raised 2000 tail flounders, puts into 60m
2The pond raise.Totally 35 days trial period.
Duration of test finds that dead fish in time pulls out, and counting, weighs.
Survey water temperature, dissolved oxygen, pH value, salinity every day; Surveyed an ammonia nitrogen in per three days.
2. test index and testing method
When 1. putting seedling and during off-test, weigh for collective of unit with the repeating groups respectively.
2. during off-test by the repeating groups ramming material, calculate the full phase and throw the amount of raising (food ration).
3. be that unit measures initial body weight in latter stage, full phase food ration, the death toll of reaching with the repeating groups, and calculate indexs such as its average growth rate, feed coefficient, surviving rate, calculate the mean value of each treatment group corresponding index with measured values such as the rate of body weight gain of repeating groups, feed coefficient, surviving rates.
4. calculation formula
Food ration (butt)=full phase food ration (weight in wet base) * 0.44
Soft particle water ratio calculates: 80% (iced trash fish) * 70% (flesh of fish water ratio)=0.56
Dry powder rate=1-0.56=0.44
Coefficient of condition is represented with the body weight (g) of consubstantiality Changyu (18.5cm).
3. test result
Table 1 blue algae fermentation nutrition A feed is to the feeding experiment result of flounder
| Project | Test 1 group | Test 2 groups | Contrast 1 group | Contrast 2 groups |
| Test fate (d) | 35 | 35 | 35 | 35 |
| Pond specification (m 2) | 60 | 60 | 60 | 60 |
| Test is mantissa just | 2000 | 2000 | 2000 | 2000 |
| Test is gross weight (kg) just | 168.8 | 170.5 | 178.6 | 240 |
| The just equal cabrage (g) of test | 84.4 | 85.3 | 89.3 | 120 |
| Dead fish tail number | 9 | 7 | 10 | 9 |
| Surviving rate (%) | 99.6 | 99.7 | 99.5 | 99.6 |
| Test last gross weight (kg) | 268.2 | 274.4 | 262 | 324.2 |
| Test end heavy (g) | 134.7 | 137.7 | 131.7 | 162.8 |
| Total net gain (kg) | 99.4 | 103.8 | 83.4 | 84.2 |
| The equal net gain of tail (g) | 50.3 | 52.4 | 42.4 | 42.8 |
| Rate of body weight gain (%) | 59.6 | 61.5 | 47.4 | 35.7 |
| Total amount of raising (kg) of throwing | 237.5 | 226.7 | 292.7 | 295.1 |
| Feed coefficient (butt) | 1.05 | 0.96 | 1.54 | 1.54 |
| The long weight e/18.5cm of consubstantiality | 137.7 | 136.5 | 121.7 | 113 |
4. biology Comprehensive Assessment
Show that the blue algae fermentation nutrition is a positive-effect to culturing flounder promotes growth effect, and effect is remarkable.Test determination is the result show, only through feeding experiment in 35 days, economic benefit is significant.
II. Nutrious fermented thing is used for the test of feeding dorking as feed
1. materials and methods
1. experimental animal and grouping: select AA seedling chicken 1200 plumages for use, be divided into test group and control group at random.Like to pull out fryer (ARBOR ACRES) increasingly, claim the AA fryer again, be the U.S. like to pull out the company of breeding increasingly (ARBOR ACRES FARM, what INC.) cultivate four is supporting white plumage fryer kind, four is to be the White Rock type, feather is white, leaf-comb.
2. test design and diet are formed:
Test group: the blue algae fermentation nutrition feed of feeding, feed formulation is: the blue algae fermentation nutrition B 98% of following embodiment 11; Stone flour 0.92%; Secondary calcium phosphate 0.85%; Salt 0.23%; Wherein take by weighing blue algae fermentation nutrition B, 27.6kg stone flour, 25.5kg secondary calcium phosphate and the 6.9kg salt of the following embodiment 11 of 2940kg respectively, be mixed with out the blue algae fermentation nutrition feed of 3000kg.
Control group: the Dorking granular feed (containing microbiotic) of feeding common, feed formulation is: corn 76.5%; Dregs of beans 18.5%; Fish meal 3.0%; Secondary calcium phosphate 0.85%; Stone flour 0.92%; Salt 0.23%; Wherein take by weighing 2295kg corn, 555kg dregs of beans, 90kg fish meal, 25.5kg secondary calcium phosphate, 27.6kg stone flour and 6.9kg salt respectively, be mixed with out the control feed of 3000kg.
Ground flat is supported, record end of term body weight, surviving rate, feed consumption rate.
3. immune programme for children: two groups of immune programme for children are identical, weak malicious seedling of the 1 age in days fabricius bursa and chicken Marek's vaccination immunity; The immunity of the new Zhi Erlian collunarium of 7 ages in days; The immunity of the weak malicious seedling drinking-water of the 17 age in days fabricius bursa; 25 age in days influenza vaccines injecting immunes.
2. test-results
1. broiler trial the results are shown in Table 2.
Table 2 blue algae fermentation nutrition B granulated feed nursing broiler chicken test comparing result
| Project | Test group | Control group |
| Seedling chicken quantity (plumage) | 600 | 600 |
| Surviving rate (%) | 96.1 | 95.7 |
| The raising fate (my god) | 50 | 50 |
| The end of term is heavy (kg) on average | 2.26 | 2.12 |
| Full phase feed consumption rate (kg) | 2780 | 2750 |
| Feedstuff-meat ratio | 2.14 | 2.25 |
2. conclusion: test group close with the control group surviving rate (being respectively 96.1% and 95.7%), test group and control group were raised the AA fryer 50 days, and mean body weight is respectively 2.26kg and 2.12kg, and the test group feedstuff-meat ratio is 2.14: 1, and the control group feedstuff-meat ratio is 2.25: 1; The test group speed of growth and feedstuff-meat ratio are better than control group.
III. Nutrious fermented thing is used to the test of feeding pork pig as feed
1. materials and methods
1. select and grouping for the examination pig: for the examination pig is the ternary hybrid pig, selects 24 of the growing swine of age in days, parity close with body weight (young pig about body weight 30kg) to test 40 days, is divided into test group and control group, every group of two repetitions, each repeats 6, and through check, initial body weight does not have significant difference.
2. test design and diet are formed:
Test group: the blue algae fermentation nutrition feed of feeding, feed formulation is: the blue algae fermentation nutrition C 98.29% of following embodiment 12; Stone flour 0.62%; Secondary calcium phosphate 0.84%; Salt 0.25%; Wherein take by weighing blue algae fermentation nutrition C, 6.2kg stone flour, 8.4kg secondary calcium phosphate and the 2.5kg salt of the following embodiment 12 of 982.9kg respectively, be mixed with out the blue algae fermentation nutrition feed of 1000kg.
Control group: the Dorking granular feed (containing microbiotic) of feeding common, feed formulation is: corn 68.89%; Dregs of beans 20.90%; Inferior powder 3.0%; Fish meal 1.5%; Wheat bran 4.0%; Secondary calcium phosphate 0.84%; Stone flour 0.62%; Salt 0.25% wherein takes by weighing 688.9kg corn, 209kg dregs of beans, 30kg powder, 15kg fish meal, 40kg wheat bran, 8.4kg secondary calcium phosphate, 6.2kg stone flour and 2.5kg salt respectively, is mixed with out the control feed of 1000kg.
2. test-results
1. the pork pig test-results sees Table 3.
Table 3 blue algae fermentation nutrition C granulated feed pork pig feeding study comparing result
| Test group | Control group | |||
| Repeat 1 | Repeat 2 | Repeat 1 | Repeat 2 | |
| Number | 6 | 6 | 6 | 6 |
| Average starting weight (kg) | 30.00 | 30.05 | 30.10 | 30.90 |
| The raising fate (my god) | 40 | 40 | 40 | 40 |
| Average end heavy (kg) | 57.80 | 58.90 | 56.90 | 58.30 |
| Total feed consumption rate (kg) | 460 | 460 | 455 | 460 |
| Average daily gain (g) | 695 | 710 | 670 | 685 |
| The material anharmonic ratio | 2.76 | 2.70 | 2.83 | 2.80 |
2. conclusion: test group average daily gain 702.5g is higher by 4% than control group 677.5g.Average material anharmonic ratio test group 2.73: 1, control group 2.815: 1, the every kg weightening finish of test group economizes feed consumption 0.085kg than control group, saves feed 3.0%, and promptly feed conversion rate improves 3.0%.
The experimental data result of blue-green algae biological organic fertilizer of the present invention is as follows:
I. use the effect test of blue-green algae biological organic fertilizer aspect the eggplant plantation
One, test is handled
Processing is respectively:
A, use the blue-green algae biological organic fertilizer A of following embodiment 18, mu applies fertilizer to the subsoil 60 kilograms, and mu imposes 10 kilograms for the first time, imposes 20 kilograms for the second time;
B, use chemical fertilizer, 30 kilograms of mu bottom application diammonium phosphate impose 30 kilograms in urea at twice between breeding time, and mu imposes 10 kilograms for the first time, imposes 20 kilograms for the second time;
C, farm manure, chemical fertilizer compounding application, promptly 2000 kilograms of bottom application farm manures, 30 kilograms of diammonium phosphate impose 30 kilograms in urea between breeding time, and mu imposes 10 kilograms for the first time, imposes 20 kilograms for the second time.
Two, test site and method
0.05 mu of every experimental plot is buried two repetitions everywhere underground.
Three, the result observes
By the observation to whole growing, showing as the plant of using blue-green algae biological organic fertilizer A, obviously to show as plant in the forward and backward phase sturdy, and the result is big and many, the single fruit weight height, and the eggplant quality is better.The overall growing way of only using chemical fertilizer B is better, but the eggplant mouthfeel is poor, and quality is low.Use the B to fertilize that only outperforms of chemical fertilizer C in conjunction with farm manure.Investigation result data such as following table 4:
The various processing of table 4 are to the eggplant effect contrast of breeding time
II. use the effect test of blue-green algae biological organic fertilizer on the net melon
One, test is handled:
Handle: spread manuer in holes 80 kilograms of every mu of blue-green algae biological organic fertilizer B that uses the embodiment of the invention 19 before transplanting seedlings;
Contrast (CK): spread manuer in holes before transplanting seedlings, use 80 kilograms of composite fertilizer'ss (N, P, K content 45%) for every mu.
Two, collecting period investigation result:
Table 5 is respectively handled single melon and is heavily reached the sugar degree contrast
| Handle | Average single melon heavy (kilogram /) | Stimulation ratio (%) | On average contain sugar (degree) | Increase sugar (degree) | Ripening degree |
| Handle | 4.66 | 13.7 | 11.95 | 3.3 | 9 one-tenth |
| CK | 4.02 | 0 | 8.65 | 0 | 7 one-tenth |
When gathering, handle every index and all reached commodity melon standard substantially, average single melon weighs 4.66 kilograms; Processing on average contains sugar 11.95 degree, and comparison is according on average containing sugared 8.65 high 3.3 degree; Handle ripening degree and reach 9 one-tenth, and the contrast ripening degree only reaches 7 one-tenth.These two indexs have shown that fully the blue-green algae biological organic fertilizer promotes that precocious function is the strongest, have necessarily to increase sugar, volume increase, the precocious function of promotion.
III. use the effect test of blue-green algae biological organic fertilizer on apple tree
One, test materials and method
1. for studying thing: apple tree.
2. for examination material: the blue-green algae biological organic fertilizer C of the embodiment of the invention 20 (containing ammonium potassium agglutinent water-loss reducer of soil)
3. test is handled
Handle 1: the tree crown projecting edge digs the wide rectangular hole of 10-15cm that is, is as the criterion with the exposed portions serve root system, uses the blue-green algae biological organic fertilizer C 1000g of the embodiment of the invention 20 in the every strain fruit tree in end 10cm place, hole, the residue earthen backfill compacting 30L that fully pours water.
Handle 2: use the blue-green algae biological organic fertilizer C 800g of the embodiment of the invention 20 with the every strain fruit tree of same quadrat method of handling 1, remain the earthen backfill compacting 30L that fully pours water.
Handle 3: use the blue-green algae biological organic fertilizer C 600g of the embodiment of the invention 20 with the every strain fruit tree of same quadrat method of handling 1, remain the earthen backfill compacting 30L that fully pours water.
Contrast: with the every strain fruit tree of same quadrat method sealing fertilizer (N, P, the K 45%) 1000g that handles 1, the residue earthen backfill compacting 30L that fully pours water.
4. test design:
Apple tree selects the above fruit tree in the best fruiting period of tree in 6 ages, and ten strains are a sub-district, and it is guard rows that five strains are stayed in the minizone.
Two, result and analysis
Table 6 is used the apple production questionnaire of the blue-green algae biological organic fertilizer C of the embodiment of the invention 20
| Handle | Special large fruit rate (%) | All kinds of fruits heavy (kg) | Per mu yield (kg) | Mu volume increase (kg) | Stimulation ratio (%) |
| Handle 1 | 82 | 32.4 | 2407 | 215 | 8.9 |
| Handle 2 | 86 | 34.2 | 2437 | 245 | 10.0 |
| Handle 3 | 78 | 29.8 | 2289 | 97 | 4.2 |
| Contrast | 73 | 26.7 | 2192 |
From above-mentioned yield result, use the processing of blue-green algae biological organic fertilizer C of the present invention and compare with contrast, volume increase is in various degree arranged, handle the heavy average 7.5kg of increasing of 2 all kinds of fruits, average mu volume increase 245kg.
IV. use the effect test of blue-green algae biological organic fertilizer on paddy rice
Table 7 is used the influence of the blue-green algae biological organic fertilizer D of the embodiment of the invention 21 to rice yield
| Handle | Every mu of spike number (ten thousand) | Number of grain per ear (individual) | Thousand seed weight (g) | Sub-district 10 2(jin) | Output/mu (jin) | Stimulation ratio (%) |
| Handle | 21.43 | 68.55 | 25.26 | 9.26 | 617.64 | 18.46 |
| CK | 18.97 | 68.73 | 23.89 | 7.55 | 503.58 | / |
| Difference | 2.46 | -0.18 | 1.37 | 1.71 | 114.04 | 18.46 |
Fermentation provided by the invention microbial bacteria composition possesses the ability to the solid blue algae fermentation, and can decompose blue-green algae colloid and organism nutritive substance by fermentation, removes objectionable impurities, promotes material to transform the deodorizing of becoming thoroughly decomposed; Can secrete organic acid, physiologically active substance and antibiotic, suppress spreading of pathogenic bacteria, crop is had short living, disease-resistant and fertilizer efficiency effect; Can secrete multiple meta-bolites, have certain Decomposition containing cellulosic material; Have fixed nitrogen, phosphorus decomposing, potassium decomposing effect, the nutritive substance that can transform in the edatope is that crop is used.
Blue algae fermentation nutrition of the present invention does not contain harmful ingredients, and has fine protein and rich in amino acid and vitamins and other nutritious components, is good animal-feed and bait.
Blue-green algae biological organic fertilizer of the present invention has the controlling crop diseases and insect pests effect, belong to the good organic matter of natural mineral, help promoting the Harmony of compound in the crop, improve fertilizer efficiency and have long-acting for nitrogen, phosphorus, potassium capability, for plant provides effective nutritive ingredient.
The characteristics of blue-green algae biological organic fertilizer of the present invention and effect:
The present invention is through repetition test research, found that the blue-green algae biological organic fertilizer that the present invention obtains, have the controlling crop diseases and insect pests effect, can promote plant growth, improve soil physical and chemical property, effectively decompose nitrogen in the soil (N), phosphorus (P), potassium (K), contain the rich potassium shale of natural mineral, contain and have or not the sodium water-holding agent, contain nutritive elements such as oligochitosan (plant amino-oligosaccharide), brown coal, the peat composed of rotten mosses, nitrogen, phosphorus, potassium, silicon, iron, magnesium.
(1) contain a large amount of antimicrobial substances in the meta-bolites of the combination of the microbial bacteria in the blue-green algae biological organic fertilizer of the present invention, phytokinin (CTK), Plant hormones regulators,gibberellins (GA3), indolylacetic acid (IAA) etc. are arranged in its meta-bolites, its productive rate proportioning reaches preferable states.Show through a large amount of experiments with to the field test of various crop, some fungies, bacterium and virus are produced immunity, can change soil microflora, promote the beneficial microorganism growth and suppress some phytopathogens, can increase the organic composition in the soil effectively, promote soil aggregate to form.
(2) the natural base-material selected for use of blue-green algae biological organic fertilizer of the present invention, for example blue-green algae, oligochitosan (plant amino-oligosaccharide), brown coal, the peat composed of rotten mosses, rich potassium shale etc. are natural, the mineral high-quality organic substances that does not contain disease and pest.Help beneficial microorganism fauna environment, reduce pollution, promote the beneficial microorganism growth and inhibition pathogenic bacteria infecting crop to crop soil.
(3) contain natural base-material in the blue-green algae biological organic fertilizer of the present invention, the rich potassium shale of mineral for example belongs to the solubility in citric acid potassium (K that can be absorbed by root system of plant
2O) and silicon (SiO
2) nutrient, can effectively improve the potassium content of crop, can improve potassium utilization rate in conjunction with the silicate-dissolving microbe among the present invention, can improve effect of potassium fertilizer and have the long-acting potassium capability that supplies, be difficult for being run off by trickle, can not cause the natural potash fertilizer of long-acting slow-release of agricultural pollution.
(4) water-holding agent that contains of blue-green algae biological organic fertilizer of the present invention, ammonium potassium agglutinent water-loss reducer of soil for example, do not contain the deleterious sodium of soil, water-holding agent itself contains the potassium element, can improve root system of plant liquid manure environment, can improve potassium utilization rate in conjunction with silicate-dissolving microbe of the present invention, prolong effect of potassium fertilizer and improve the supply potassium capability of potash fertilizer in the plant growth intermediary and later stages.Water-holding agent can be decomposed into carbonic acid gas, water and nitrogen under the effect of sunlight, useful and harmless to soil, can water saving and minimizing dressing fertilizer amount, the growth of suitable drought environment crop.
The viable count of blue-green algae biological organic fertilizer of the present invention is 0.5～100,000,000/every gram.The organotrophy component content is respectively: organic 30～50%, humic acid 10～20%, nitrogen 8～12%, phosphorus 2～4%, potassium 6～8%, silicon 2～3%, iron 0.5～1%, but also contain zinc, boron, molybdenum, magnesium, etc. trace element.
Below will the invention will be further described by embodiment, these descriptions are not to do further to limit to content of the present invention.One skilled in the art will understand that to be equal to replacement to what the technical characterictic of content of the present invention was done, or corresponding the improvement, still belong within protection scope of the present invention.
Embodiment
Embodiment 1: the fermentation preparation of microbial bacteria composition A
(1) glass measure of preparation utensil: 1000g shakes can container.
Glass measure with 1000g measures with microbial bacteria composition A fermentation.The operation room temperature must not be higher than 20 ℃.
(2) fermentation with the weight proportion of each bacterial classification among the microbial bacteria composition A is:
Plant lactobacillus: subtilis: Bacillus licheniformis: swamp Rhodopseudomonas=20g: 10g: 10g: 60g (wherein employed each bacterial classification is the microorganism of the strain culturing of selling on the market)
(3) preparation process: 20g plant lactobacillus, 10g subtilis, 10g Bacillus licheniformis and 60g swamp Rhodopseudomonas put into shake can container, thorough mixing promptly gets and ferments with microbial bacteria composition A.
Embodiment 2: the fermentation preparation of microbial bacteria composition B
Adopt utensil and the step of embodiment 1 to prepare fermentation microbial bacteria composition B, difference is that the weight proportion that ferments with each bacterial classification among the microbial bacteria composition B is:
Plant lactobacillus: subtilis: Bacillus licheniformis: swamp Rhodopseudomonas=30g: 10g: 10g: 50g.
Embodiment 3: the fermentation preparation of microbial bacteria composition C
Adopt utensil and the step of embodiment 1 to prepare fermentation microbial bacteria composition C, difference is that the weight proportion that ferments with each bacterial classification among the microbial bacteria composition C is:
Plant lactobacillus: subtilis: Bacillus licheniformis: swamp Rhodopseudomonas=30g: 15g: 15g: 40g.
Embodiment 4: the fermentation preparation of microbial bacteria composition D
Adopt utensil and the step of embodiment 1 to prepare fermentation microbial bacteria composition D, difference is that the weight proportion that ferments with each bacterial classification among the microbial bacteria composition D is:
Plant lactobacillus: subtilis: Bacillus licheniformis: swamp Rhodopseudomonas=35g: 20g: 15g: 30g.
Embodiment 5: the preparation of blue-green algae, the peat composed of rotten mosses and stalk base-material
(1) blue-green algae base-material:
The water content of the blue-green algae of extracting in the water body in lake is adjusted to 70～80%, and removing behind the foreign material is the blue-green algae base-material.
(2) peat composed of rotten mosses, stalk base-material:
The peat composed of rotten mosses, stalk are made 100 purpose powders respectively,, obtain various base-materials after drying moisture≤10% then with various powder high temperature (125 ℃) sterilization 4 hours.
Embodiment 6: the preparation of blue-green algae biological bacteria starter A
(1) equipment: pot type stirrer, metering fluid filling pump (band spray header).
(2) weight proportion of the composition of blue-green algae biological bacteria starter A and each component of containing is:
Fermentation microbial bacteria composition A: the peat composed of rotten mosses: stalk=10kg: 10kg: 80kg.
(3) preparation process:
The 10kg peat composed of rotten mosses that embodiment 5 is prepared and 80kg stalk are packed in the pot type stirrer, machine to rotate and stir, with the metering fluid filling pump 10kg of embodiment 1 fermentation is sprayed in the pot type stirrer with microbial bacteria composition A, being deployed into total water content with distilled water after stirring is 25% compound.Drying (temperature is no more than 60 ℃) is ground into 100 orders and promptly gets blue-green algae biological bacteria starter A of the present invention to moisture≤10%.
Embodiment 7: the preparation of blue-green algae biological bacteria starter B
Adopt equipment and the step of embodiment 6 to prepare blue-green algae biological bacteria starter B, difference is that the composition of blue-green algae biological bacteria starter B and the weight proportion of each component are:
Fermentation microbial bacteria composition B: the peat composed of rotten mosses: stalk=20kg: 10kg: 70kg.
Embodiment 8: the preparation of blue-green algae biological bacteria starter C
Adopt equipment and the step of embodiment 6 to prepare blue-green algae biological bacteria starter C, difference is that the composition of blue-green algae biological bacteria starter C and the weight proportion of each component are:
Fermentation microbial bacteria composition C: the peat composed of rotten mosses: stalk=30kg: 20kg: 50kg.
Embodiment 9: the preparation of blue-green algae biological bacteria starter D
Adopt equipment and the step of embodiment 6 to prepare blue-green algae biological bacteria starter D, difference is that the composition of blue-green algae biological bacteria starter D and the weight proportion of each component are:
Fermentation microbial bacteria composition D: the peat composed of rotten mosses: stalk=30kg: 30kg: 40kg.
Embodiment 10: the preparation of blue algae fermentation nutrition A
(1) equipment: horizontal spiral stirs fermentation vat.
(2) weight proportion of the composition of blue algae fermentation nutrition A and each component of containing is:
Blue-green algae biological bacteria starter A: blue-green algae=10kg: 90kg.
(3) preparation process:
90kg blue-green algae in the base-material of embodiment 5 preparations is put into horizontal spiral stir fermentation vat, add the 10kg blue-green algae biological bacteria starter A of embodiment 6 preparations and the rotation stirring of machining, temperature remains between 35 ℃～65 ℃, ferments 96 hours, and per 4 hours stirrings once.After treating that blue algae fermentation is good, drying (temperature is no more than 60 ℃) is ground into 100 orders and promptly gets blue algae fermentation nutrition A of the present invention to moisture≤10%.
Embodiment 11: the preparation of blue algae fermentation nutrition B
Adopt equipment and the step of embodiment 10 to prepare blue algae fermentation nutrition B, difference is that the composition of blue algae fermentation nutrition B and the weight proportion of each component are:
Blue-green algae biological bacteria starter B: blue-green algae=20kg: 80kg.
Embodiment 12: the preparation of blue algae fermentation nutrition C
Adopt equipment and the step of embodiment 10 to prepare blue algae fermentation nutrition C, difference is that the composition of blue algae fermentation nutrition C and the weight proportion of each component are:
Blue-green algae biological bacteria starter C: blue-green algae=30kg: 70kg.
Embodiment 13: the preparation of blue algae fermentation nutrition D
Adopt equipment and the step of embodiment 10 to prepare blue algae fermentation nutrition D, difference is that the composition of blue algae fermentation nutrition D and the weight proportion of each component are:
Blue-green algae biological bacteria starter D: blue-green algae=40kg: 60kg.
Embodiment 14: the preparation of organism base material A
(1) equipment: drum mixer.
(2) weight proportion of the composition of organism base material A and each component of containing is:
Oligochitosan: brown coal: rich potassium shale: wilkinite=0.5kg: 10kg: 79.5kg: 10kg.
(3) preparation process:
1. the processing of organism base-material:
Oligochitosan, brown coal, rich potassium shale are become 100 purpose base-materials with the wilkinite separated pulverizing, with base-material high temperature (125 ℃) sterilization 4 hours, through being cooled to normal temperature promptly.
2. the preparation of organism base material A:
0.5kg oligochitosan, 10kg brown coal, 79.5kg richness potassium shale and the 10kg wilkinite of above-mentioned preparation are put into drum mixer stir, the compound oven dry is promptly got the organism base material A to (temperature is no more than 60 ℃) moisture≤25%.
Embodiment 15: the preparation of organism base-material B
Adopt equipment and the step of embodiment 14 to prepare organism base-material B, difference is that the composition of organism base-material B and the weight proportion of each component are:
Oligochitosan: brown coal: rich potassium shale: wilkinite=1kg: 20kg: 70kg: 9kg.
Embodiment 16: the preparation of organism base-material C
Adopt equipment and the step of embodiment 14 to prepare organism base-material C, difference is that the composition of organism base-material C and the weight proportion of each component are:
Oligochitosan: brown coal: rich potassium shale: wilkinite=5kg: 25kg: 65kg: 5kg.
Embodiment 17: the preparation of organism base-material D
Adopt equipment and the step of embodiment 14 to prepare organism base-material D, difference is that the composition of organism base-material D and the weight proportion of each component are:
Oligochitosan: brown coal: rich potassium shale: wilkinite=10kg: 30kg: 50kg: 10kg.
Embodiment 18: the preparation of blue-green algae biological organic fertilizer A
(1) equipment: fertilizer extruding nodulizer, drum mixer.
(2) weight proportion of the composition of blue-green algae biological organic fertilizer A and each component of containing is:
Blue algae fermentation nutrition A: organism base material A: ammonium potassium agglutinent water-holding agent=85kg: 14kg: 1kg.
(3) preparation process:
1. the processing of water-holding agent:
Water-holding agent is ground into 100 orders, with its high temperature (125 ℃) sterilization 4 hours, through being cooled to normal temperature promptly.
2. the granules preparation of blue-green algae biological organic fertilizer A:
The 14kg organism base material A of the 85kg blue algae fermentation nutrition A of embodiment 10 preparation, embodiment 14 preparations is put into drum mixer to stir; compound is dried to (temperature is no more than 60 ℃) moisture≤5%; when mixture temperature drops to 35 ℃, it is transferred in the drum mixer; machine to rotate to stir and add 1kg ammonium potassium agglutinent water-holding agent remix stirring 10 minutes after 10 minutes; transfer to the particle of making diameter 3～5mm in the fertilizer extruding nodulizer immediately, promptly get blue-green algae biological organic fertilizer A of the present invention.
Embodiment 19: the preparation of blue-green algae biological organic fertilizer B
Adopt equipment and the step of embodiment 18 to prepare blue-green algae biological organic fertilizer B, difference is that the composition of blue-green algae biological organic fertilizer B and the weight proportion of each component are:
Blue algae fermentation nutrition B: organism base-material B: ammonium potassium agglutinent water-holding agent=85kg: 11kg: 4kg.
Embodiment 20: the preparation of blue-green algae biological organic fertilizer C
Adopt equipment and the step of embodiment 18 to prepare blue-green algae biological organic fertilizer C, difference is that the composition of blue-green algae biological organic fertilizer C and the weight proportion of each component are:
Blue algae fermentation nutrition C: organism base-material C: ammonium potassium agglutinent water-loss reducer of soil=79kg: 25kg: 6kg.
Embodiment 21: the preparation of blue-green algae biological organic fertilizer D
Adopt equipment and the step of embodiment 18 to prepare blue-green algae biological organic fertilizer D, difference is that the composition of blue-green algae biological organic fertilizer D and the weight proportion of each component are:
Blue algae fermentation nutrition D: organism base-material D: ammonium potassium agglutinent water-holding agent=50kg: 40kg: 10kg.
Claims (10)
1. fermentation microbial bacteria composition, it contains the bacterial classification of following parts by weight proportioning:
10～40 parts of plant lactobacilluss,
5～20 parts of subtilises,
5～20 parts of Bacillus licheniformis,
30～60 parts of Rhodopseudomonas palustriss.
2. fermentation microbial bacteria composition according to claim 1, the parts by weight proportioning of wherein said each bacterial classification is:
20～35 parts of plant lactobacilluss,
10～20 parts of subtilises,
10～15 parts of Bacillus licheniformis,
30～60 parts of Rhodopseudomonas palustriss.
3. blue-green algae biological bacteria starter that contains claim 1 or 2 each described fermentations with the microbial bacteria compositions, its each component by following parts by weight proportioning is prepared from:
10～30 parts of microbial bacteria compositions of fermentation,
50～110 parts of base-materials;
Wherein said base-material is selected from the mixture of the peat composed of rotten mosses and stalk, and its mixed weight umber proportioning is:
10～30 parts of the peats composed of rotten mosses,
40～80 parts of stalks.
4. blue algae fermentation nutrition that contains the described blue-green algae biological bacteria of claim 3 starter, its each component by following parts by weight proportioning is prepared from:
10～40 parts of blue-green algae biological bacteria starters,
60～90 parts of blue-green algaes.
5. one kind contains the nutraceutical blue-green algae biological organic fertilizer of the described blue algae fermentation of claim 4, and its each component by following parts by weight proportioning is prepared from:
55～85 parts of blue algae fermentation nutrition,
11～40 parts of organism base-materials,
1～10 part of water-holding agent;
Wherein said water-holding agent is selected from one or more in ammonium potassium agglutinent water-loss reducer of soil, non-metallic minerals water-loss reducer of soil, polyacrylamide water-holding agent, polyacrylate and the polyacrylamide interpolymer water-loss reducer of soil;
Described organism base-material is oligochitosan, brown coal, rich potassium shale and bentonitic mixture, and its parts by weight proportioning is:
0.5～10 part of oligochitosan,
10～30 parts in brown coal,
40～80 parts in rich potassium shale,
1～10 part of wilkinite.
6. the preparation method of the described blue-green algae biological bacteria of claim 3 starter, it may further comprise the steps:
(1) base-material of 50～110 parts of weight is made powder, the high-temperature sterilization postcooling;
(2) the microbial bacteria composition of 10～30 parts of weight of adding stirs and makes blue-green algae biological bacteria starter, and total water content is 25%.
7. nutraceutical preparation method of the described blue algae fermentation of claim 4, it may further comprise the steps:
(1) initial water content of adjustment blue-green algae is removed foreign material; Wherein the initial water content of blue-green algae is controlled at 70%～80%;
(2) the blue-green algae biological bacteria starter with 10～40 parts of weight adds in the blue-green algae of 60～90 parts of weight; With
(3) stir, and the temperature bottom fermentation between 35 ℃～65 ℃, oven dry; The time of wherein said fermentation is 96～240 hours, and the stirring time during the fermentation is 4～16 hours.
8. the preparation method of the described blue-green algae biological organic fertilizer of claim 5, it may further comprise the steps:
(1) the organism base-material of 11～40 parts of weight is pulverized high-temperature sterilization, cooling;
(2) the blue algae fermentation nutrition with 55～85 parts of weight adds in the described organism base-material, stirs, dries;
(3) water-holding agent that adds 1～10 part of weight mixes stirring again, adopts the preparation technology who produces particle fertilizer to make described blue-green algae biological organic fertilizer;
The preparation of wherein said organism base-material may further comprise the steps:
(1) oligochitosan, brown coal, rich potassium shale and wilkinite are made powder respectively, the high-temperature sterilization postcooling;
(2) oligochitosan, the brown coal of 10～30 parts of weight, the rich potassium shale of 40～80 parts of weight and the wilkinite of 1～10 part of weight with 0.5～10 part of weight of above-mentioned preparation mixes, and stirs and makes the organism base-material.
9. the described blue algae fermentation nutrition of claim 4 is used alone or in combination the purposes as animal-feed and bait.
10. the using method of the described blue-green algae biological organic fertilizer of claim 5, it comprises:
(1) fertilizer of growing seedlings applies 2～3 kilograms of blue-green algae biological organic fertilizers and is used for growing seedlings of crop, vegetables, plant by per 10 square meters bed of growing seedlings;
(2) base manure imposes in the soil before crop, vegetables, the plant fixing by every mu of 80～100 kilograms of blue-green algae biological organic fertilizers; With
(3) topdress, dash as topdressing of crop, vegetables, plant by every mu of 20～30 kilograms of blue-green algae biological organic fertilizers and execute.
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