CN105230998A - Composite micro-ecological feed additive and preparation method thereof - Google Patents
Composite micro-ecological feed additive and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a composite micro-ecological feed additive and a preparation method thereof. The preparation method comprises the following steps: preparing a traditional Chinese medicine-fermented component A; preparing dried powder of a nostoc fermentation product; then fully mixing 10-18 parts of the component A, 0.5-1 part of the dried powder of the nostoc fermentation product, 0.1 part of nanometer talc powder, 0.08 part of beta-mannanase, 0.1 part of beta-glucanase, 0.15 part of cellulase, 0.05 part of pectinase and 0.1 part of xylanase, thereby obtaining the final product. The composite micro-ecological feed additive of the invention has natural sources, low cost and wide applicability. The composite micro-ecological feed additive can be used for various common livestock, such as ruminants, pigs and even birds. The composite micro-ecological feed additive is capable of effectively reinforcing nutrient values of the basic feeds, so as to improve animal growth performance, ensure animal health and save feed cost; and the composite micro-ecological feed additive has obvious effects in multiple aspects, such as the aspect of improving animal product quality and the like. The composite micro-ecological feed additive has especially good gastrointestinal conditioning functions, and the technical effect of completely avoiding diarrhea is achieved by adding the composite micro-ecological feed additive into various animal feeds. Moreover, an unanticipated effect of greatly prolonging the shelf life of the composite micro-ecological feed additive is found after adding a small amount of talc powder; thus, the composite micro-ecological feed additive can be stored for 24 months at normal temperature with the number of living bacteria and the nutrient compositions therein basically unchanged.
Description
Technical field
The present invention relates to a kind of composite microorganism type forage additives and preparation method thereof, belong to feed additive field.
Background technology
Feed addictive refers at Feed Manufacturing processing, a small amount of or micro substance that adds in use procedure, in feed consumption seldom but effect significantly.Feed addictive is the raw material that modern feed industry must use, and to strengthening basal feed nutritive value, improve breeding performonce fo animals, ensure animal health, save feed cost, improving the aspects such as livestock products quality has obvious effect.
Along with the enhancing of health of people consciousness, requirement is also improved to the quality of various animal products and security, therefore, while improving food conversion ratio and livestock and poultry grow fast, develop the green feed additive of alternative antibiotic and chemical synthetic drug, thus ensure animal products safety, become the problem that present feed industry development will solve.
The bark of eucommia is China's traditional Chinese medicine material, and from feedstuff industry development and the requirement of green cultivation, its superior antibacterial growth promotion performance of folium cortex eucommiae has obtained everybody extensive accreditation.Shandong Long Chang animal health-care product Co., Ltd once entrusted the research of Duo Suo university to eucommia leaf extract to do many experiments, test confirms, in eucommia leaf extract, main component chlorogenic acid has broad-spectrum antibacterial, resisting stress, anti-oxidant, scavenging free radicals and develop immunitypty, the effects such as alternative part antibiotic, be a kind of efficient, environmental protection, safely, the green feed additive that has no side effect.
Also occur now that additive for microbe feedstuff is a kind of microbial product replacing or balance one or more fungus strains in the animal ecosystem.Say in the narrow sense, it is that one can excite own beneficial culture propagation to increase, the microbial product of withstand deleterious fungus strain growth simultaneously.
Wherein Traditional Chinese drug microecology feed addictive contains a large amount of beneficial bacteriums (biodiasmin, Bifidobacterium, bacillus), complex enzyme, chelating peptide, enters after in livestock and poultry body as feed by de-mould dose etc., can breed rapidly, drop in the metabolin of bacterial classification on the one hand and enterogenous endotoxin, inhibit the growth of other harmful flora, in host, define normal microbial flora on the other hand, for host synthesizes main vitamin, provide the invasion of nutrition and prevention pathogenic bacteria.
The present invention aims to provide a kind of composite microorganism type forage additives and preparation method thereof, and its source is natural, with low cost, and applicability is wide, may be used for multiple common poultry, as ruminant, and pig, or even bird.It can efficient hardening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, saves feed cost, has obvious effect in many-sides such as improving livestock products quality.
Summary of the invention
Based on the technical problem that background technology exists, the present invention is directed to background technology Problems existing, provide a kind of composite microorganism type forage additives and preparation method thereof, its source is natural, addition is few, and applicability is wide, may be used for multiple common poultry, as ruminant, pig, or even bird.It can efficient hardening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, saves feed cost, has obvious effect in many-sides such as improving livestock products quality.
Object of the present invention is achieved through the following technical solutions:
A kind of preparation method of composite microorganism type forage additives:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.5-0.8 part trehalose, 0.4-0.9 part tourmaline powder, 10-13 part folium cortex eucommiae, 5-8 part hawthorn, 0.5-0.8 part Radix Astragali, 2-3 part Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 50-55 part nostoc, cleaning, wears into meal with grinder, then uses 90-95 DEG C of hot-water soak 50-85min, pull out after draining, add 10-12 part normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1-1.5%, keep temperature 38 DEG C, fermentation 52-58 hour, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus subtilis: bacillus licheniformis: bacillus coagulans: Corynebacterium glutamicum is: the proportions of 0.8:1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 10-18 part component A, 0.5-1 part nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix both finished product.
Described enzymatic activity data are:
'beta '-mannase: 20000-25000U/kg, zytase: 1.2 × 10
6u/kg; 1,4 beta-glucanase: 6.7 × 10
5u/kg, pectase: 6.0 × 10
5u/kg; Cellulase: 9.0 × 10
6u/kg
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
The compatibility of Chinese herbal feed additive both can select simple, also the compound that multi-flavor medicine forms can be selected, but not the simple rough combination of some medicines, without " method " nothing " side " nothing " compatibility ", but under the guidance of Chinese veterinarian's theory, according to inherent laws and the feature thereof of Chinese veterinarian's pharmacy, on theory contact practical basis, consider that compatibility of drugs gets up to use by the performance of modern medicines itself selectively, give full play to the mass action of medicine.Medicine for the concrete condition of animal, according to certain principle, should combine, just defines prescription by the prescription of Chinese herbal medicine selectively.This principle is said in " Plain Questions to very will discuss greatly ": " main disease be monarch, what help monarch is minister, answer minister for making.Monarch one minister two, system also little; Monarch one minister three helps five, among system also; Monarch one minister three helps nine, makes also big." principle of Here it is herbal prescription.The principles of formulating prescriptions of Chinese herbal medicine may be summarized to be monarch, minister, help, make, be referred to as now more main and auxiliary, help, make four aspects.According to characteristic and the major function of various Chinese herbal medicine, adopt extraction, its active component of concentrated natural plant, screening is optimization of C/C composites also, improve and optimize traditional Chinese herbal and crude drugs preparations technique, develop and can strengthen domestic animal immunologic function, improve its survival rate, promote that it grows, there is the compound Chinese herbal medicinal feed additive of the effects such as well antiviral, sterilization, desinsection.
Nostoc (latin name: Pogostemonauricularius (L.) Kassk; Formal name used at school: intend spherical nostoc) be commonly called as beauty's rice, sky rosetangle, loosestrife, water auricularia auriculajudae, field auricularia auriculajudae.Nostoc Cyanophyceae, nostocaceae.Frond is colloidal substance, spherical or other are irregularly shaped, blue-green or yellowish-brown.Cell is spherical, is linked to be beads shape colony, outsourcing colloid by most cell.Heterocyst is positioned at the iuntercellular of filament, and young filament is often positioned at top.Most generation akinete during breeding, often for concatenating into chain.In green time moistening, in grey black after dry.Between attached submersed sandstone or on dark and damp earth.All have distribution all over China, with Sichuan produce the most famous.Edible.There is nitrogen fixing capacity.Several amino acids containing needed by human and polysaccharide isoreactivity material.Have relieve inflammation or internal heat, improving eyesight, the therapeutic efficiency such as anti-ageing, anti-infective.
The present invention is on the basis of Chinese traditional herbs theory, and in conjunction with modernization bio-fermentation process, preparing can efficient hardening basal feed nutritive value, improve breeding performonce fo animals, ensure the composite microorganism type forage additives of animal health, it contains multiple Chinese herbal medicine effective ingredients, active bacteria, enzyme etc.And the present invention is and novelty adds a small amount of nanomete talc powder, can greatly extend the shelf life, under normal temperature, storage can reach 24 months.
Usefulness of the present invention is:
1) microbial forage additive of the present invention is drawn materials natural without any side effects, not only can strengthen the physique of animal, immunity, stress ability, and noresidue, harmless.
2) composite microorganism type forage additives of the present invention, it contains multiple Chinese herbal medicine effective ingredients, active bacteria, enzyme etc.And the present invention is and novelty adds a small amount of nanomete talc powder, can greatly extend the shelf life, under normal temperature, storage can reach 24 months.
3) composite microorganism type forage additives applicability of the present invention is wide, is applicable to comprise the many animals such as ruminant, even comprises bird.
Detailed description of the invention
The present invention's enzymatic activity data used are:
'beta '-mannase: 20000-25000U/kg, zytase: 1.2 × 10
6u/kg; 1,4 beta-glucanase: 6.7 × 10
5u/kg, pectase: 6.0 × 10
5u/kg; Cellulase: 9.0 × 10
6u/kg.
Embodiment 1:
The preparation method of composite microorganism type forage additives:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.5 portion of trehalose, 0.9 part of tourmaline powder, 10 parts of folium cortex eucommiaes, 8 portions of hawthorn, 0.5 part of Radix Astragali, 3 parts of Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 50 portions of nostoc, cleaning, wears into meal with grinder, then uses 95 DEG C of hot-water soak 50min, pull out after draining, add 12 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1%, keep temperature 38 DEG C, ferment 58 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus subtilis: bacillus licheniformis: bacillus coagulans: Corynebacterium glutamicum is: the proportions of 0.8:1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 10 parts of component A, 1 part of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Embodiment 2:
The preparation method of composite microorganism type forage additives:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.8 portion of trehalose, 0.4 part of tourmaline powder, 13 parts of folium cortex eucommiaes, 5 portions of hawthorn, 0.8 part of Radix Astragali, 2 parts of Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 55 portions of nostoc, cleaning, wears into meal with grinder, then uses 90 DEG C of hot-water soak 85min, pull out after draining, add 10 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.5%, keep temperature 38 DEG C, ferment 52 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus subtilis: bacillus licheniformis: bacillus coagulans: Corynebacterium glutamicum is: the proportions of 0.8:1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 18 parts of component A, 0.5 part of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Embodiment 3:
The preparation method of composite microorganism type forage additives:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.6 portion of trehalose, 0.8 part of tourmaline powder, 11 parts of folium cortex eucommiaes, 7 portions of hawthorn, 0.6 part of Radix Astragali, 3 parts of Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 51 portions of nostoc, cleaning, wears into meal with grinder, then uses 94 DEG C of hot-water soak 60min, pull out after draining, add 11 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.1%, keep temperature 38 DEG C, ferment 57 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus subtilis: bacillus licheniformis: bacillus coagulans: Corynebacterium glutamicum is: the proportions of 0.8:1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 11 parts of component A, 0.9 part of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Embodiment 4:
The preparation method of composite microorganism type forage additives:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.7 portion of trehalose, 0.5 part of tourmaline powder, 12 parts of folium cortex eucommiaes, 6 portions of hawthorn, 0.7 part of Radix Astragali, 2 parts of Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 54 portions of nostoc, cleaning, wears into meal with grinder, then uses 91 DEG C of hot-water soak 70min, pull out after draining, add 12 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.4%, keep temperature 38 DEG C, ferment 53 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus subtilis: bacillus licheniformis: bacillus coagulans: Corynebacterium glutamicum is: the proportions of 0.8:1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 17 parts of component A, 0.6 part of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Embodiment 5:
The preparation method of composite microorganism type forage additives:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.5 portion of trehalose, 0.7 part of tourmaline powder, 10 parts of folium cortex eucommiaes, 8 portions of hawthorn, 0.5 part of Radix Astragali, 3 parts of Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 52 portions of nostoc, cleaning, wears into meal with grinder, then uses 93 DEG C of hot-water soak 80min, pull out after draining, add 10 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.2%, keep temperature 38 DEG C, ferment 56 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus subtilis: bacillus licheniformis: bacillus coagulans: Corynebacterium glutamicum is: the proportions of 0.8:1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 12 parts of component A, 0.8 part of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Embodiment 6:
Nostoc does not carry out fermenting in contrast:
The preparation method of composite microorganism type forage additives:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.8 portion of trehalose, 0.6 part of tourmaline powder, 13 parts of folium cortex eucommiaes, 5 portions of hawthorn, 0.8 part of Radix Astragali, 2 parts of Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc dry powder is prepared:
Get nostoc, cleaning, wears into meal with grinder, dries, grinds to form fine powder;
(3) get 16 parts of component A, 0.7 part of nostoc dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Embodiment 7 pig feeding effect is tested and assessed:
Principle according to " kind is identical, body weight is close, male and female half and half, parity consistent " chooses the DLY tri-crossbreeding 350 that body weight is about 60kg, and be divided into 7 groups at random, often group establishes 5 repetitions, each repetition 10 pigs.
Control group is Conventional antibiotic control group, and other groups add the biology feed additive of the present invention of 0.1% respectively in basal diet.The initial counterpoise statistical analysis group difference of each test group pig is not remarkable.
Daily ration is corn, wheat, bean pulp type.The composite microorganism type forage additives of 0.1% is added in basal diet.
The first prerun 7d of test pig after grouping, the feeding and management condition of preliminary trial period is identical with experimental period.Within preliminary trial period, expelling parasite and immunization inocultation are carried out to test pig.Feeding manner adopts group feeding, and every day, feeding 2 times, freely drank water.Other routinely management method carry out.Experimental period is 30d.
Slaughter determining.Selecting body weight to reach the test pig of about 90kg from often organizing each repetition after off-test, butchering after fasting 24h under freely drinking water.Butcher by " national meat cooperative groups amendment scheme " (1987).Weigh before government official, measure and calculate the indexs such as lean meat percentage.
Meat measures.Measure by the 2nd national Meat research experience exchanging meeting amendment scheme " Meat assessment method " (1987).
Tenderness (shearing force): measure with C-LM25 tender degree device, detailed assay method is see " pig produces and learns ".
Taste qualification: to meat sample after salt-free water white cut, loads vegetable dish, processes expert do the trial test of flavour, succulence and soup taste by 20 livestock products.Each index removes 1 best result and 1 minimum point.
Ten point system standards of grading: more than 8.5 points is good, 8.5 ~ 7.0 be divided into medium, 7.0 ~ 6.0 be divided into bad.
Result is as shown in the table.
Table 1
| Group | Daily gain | There is head number in diarrhoea | Dressing percentage (%) | Lean meat percentage (%) |
| (g) | ||||
| Embodiment 1 | 1012.3 | 0 | 78.1 | 68.8 |
| Embodiment 2 | 967.9 | 0 | 76.9 | 69.1 |
| Embodiment 3 | 1005.1 | 0 | 78.0 | 69.7 |
| Embodiment 4 | 979.2 | 0 | 77.4 | 68.9 |
| Embodiment 5 | 995.4 | 0 | 77.1 | 69.8 |
| Embodiment 6 | 798.5 | 8 | 74.2 | 64.8 |
| Blank group | 763.9 | 10 | 73.3 | 62.3 |
As can be seen here, the biology feed additive of the present invention adding 0.1% in basal diet can play good facilitation to the growth of pig.
Embodiment 8 milking cow feeding effect is tested and assessed:
Choose 350 milk cow in lactation period according to the principle of " kind is identical, body weight is close ", be divided into 7 groups at random, often group establishes 5 repetitions, each repetition 10 cow head.
Control group is Conventional antibiotic control group, and other groups add the biology feed additive of the present invention of 0.2% respectively in basal diet.
Every leading indicator statistical analysis group difference of each test group milk cow is not remarkable.
Feeding manner adopts group feeding, and every day, feeding 2 times, freely drank water.Other routinely management method carry out.Experimental period is 30d.
Result is as shown in table 2 below.
Table 2
As can be seen here, the biology feed additive of the present invention adding 0.2% in basal diet can play good facilitation to the growth of pig.
Embodiment 9 Cherry Village Ducks feeding effect is tested and assessed:
Test site is carried out in duck field, Zhangjiagang one.Test selection 700 health, the Cherry Village Ducks that normal, the date of birth is close.According to body weight, hereditary consistent principle, be divided into 7 groups at random, often organize 5 repetitions, each repetition 20; Test meat duck adopts the two-stage to raise (in earlier stage, later stage), each phase feed of feeding respectively, and breeding cycle is about 42 days; When on-test, off-test, should weigh on an empty stomach early morning, the individual weight of record test duck, and record the feed consumption of each group of test duck and dead number of elements.
The composite microorganism type forage additives of 0.05% is added in common daily ration.
Result is as shown in table 3 below:
Table 3
As can be seen here, the biology feed additive of the present invention adding 0.05% in basal diet can play good facilitation to the growth of pig.
Visible, composite microorganism type forage additives source of the present invention is natural, with low cost, and applicability is wide, may be used for multiple common poultry, as ruminant, and pig, or even bird.It can efficient hardening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, saves feed cost, has obvious effect in many-sides such as improving livestock products quality.Especially its stomach conditioning effect is splendid, adds the technique effect all reaching 0 diarrhoea in many animals.In addition, unexpected discovery adds a small amount of talcum powder and can greatly extend the shelf life, and preserve 24 months under normal temperature, its number of viable and nutritional cost do not change substantially.
The above; be only the present invention's preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (6)
1. a preparation method for composite microorganism type forage additives, its step is as follows:
As described belowly be mass parts;
(1) component A is prepared:
Get 0.5-0.8 part trehalose, 0.4-0.9 part tourmaline powder, 10-13 part folium cortex eucommiae, 5-8 part hawthorn, 0.5-0.8 part Radix Astragali, 2-3 part Radix Angelicae Sinensis, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 5 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: Bifidobacterium: yellow Ruminococcus is: the proportions of 1:1.5:0.2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 50-55 part nostoc, cleaning, wears into meal with grinder, then uses 90-95 DEG C of hot-water soak 50-85min, pull out after draining, add 10-12 part normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1-1.5%, keep temperature 38 DEG C, fermentation 52-58 hour, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus subtilis: bacillus licheniformis: bacillus coagulans: Corynebacterium glutamicum is: the proportions of 0.8:1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 10-18 part component A, 0.5-1 part nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.08 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.15 part of cellulase, 0.05 part of pectase, 0.1 part of zytase fully mix both finished product.
2. the preparation method of composite microorganism type forage additives according to claim 1, is characterized in that:
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
3. the composite microorganism type forage additives for preparing of the preparation method of the described composite microorganism type forage additives of claim 1-2.
4. composite microorganism type forage additives according to claim 3 is for the application of pig of feeding.
5. composite microorganism type forage additives according to claim 3 is used for the application of feeding ruminants.
6. composite microorganism type forage additives according to claim 3 is for the application of bird of feeding.
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| CN105535286A (en) * | 2016-01-17 | 2016-05-04 | 新乡医学院第一附属医院 | Traditional Chinese medicine composition for resisting tumors in internal medicine |
| CN105533188A (en) * | 2016-01-25 | 2016-05-04 | 内蒙古民族大学 | Economic feed additive for promoting growth of sheep |
| CN105535067A (en) * | 2016-01-30 | 2016-05-04 | 新乡医学院 | Pharmaceutical composition for treating diabetes and preparation method thereof |
| CN105582220A (en) * | 2016-02-06 | 2016-05-18 | 济南市第三人民医院 | Preparation method of traditional Chinese medicine for treating diabetic nephropathy |
| CN105707435A (en) * | 2016-01-25 | 2016-06-29 | 长沙满旺生物工程有限公司 | Preparation method of antibiotic-free selenium-enriched livestock feed additive |
| CN105727015A (en) * | 2016-02-06 | 2016-07-06 | 济南市第三人民医院 | Composition for regulating blood lipid and blood pressure and preparation method thereof |
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| CN105727015A (en) * | 2016-02-06 | 2016-07-06 | 济南市第三人民医院 | Composition for regulating blood lipid and blood pressure and preparation method thereof |
| CN105876132A (en) * | 2016-04-23 | 2016-08-24 | 连云港恒图农牧开发有限公司 | Preparation method of silkworm chrysalis-cordyceps militaris fermentation product feed additive |
| CN108013247A (en) * | 2017-12-28 | 2018-05-11 | 河南新明磊生物科技有限公司 | A kind of strong scattered mixture of fermented type, preparation method and applications |
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Application publication date: 20160113 |