CN107047972B - Functional feed added with water bloom blue algae extract - Google Patents
Functional feed added with water bloom blue algae extract Download PDFInfo
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- CN107047972B CN107047972B CN201710270536.9A CN201710270536A CN107047972B CN 107047972 B CN107047972 B CN 107047972B CN 201710270536 A CN201710270536 A CN 201710270536A CN 107047972 B CN107047972 B CN 107047972B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a functional feed added with a bloom-forming cyanobacteria extract, which comprises the following components in parts by weight: 50-80 parts of bran, 35-55 parts of corn flour, 15-30 parts of waterweed powder, 3-9 parts of water-blooming cyanobacteria extract, 5-15 parts of yellow wine lees, 10-20 parts of fish meal, 20-25 parts of auxiliary feed components and 2-5 parts of vitamins. The feed formula is added with the water-blooming cyanobacteria extract, the water-blooming cyanobacteria extract is used as an additive, the escherichia coli, pseudomonas aeruginosa and staphylococcus aureus can be effectively killed, the sterilization rate is over 90 percent, the extract of the water-blooming cyanobacteria after enzymolysis contains phycobiliprotein, and the water-blooming cyanobacteria has good cell oxidation resistance and immunity enhancing function after being added into the feed through test verification.
Description
Technical Field
The invention relates to the field of feeds, and in particular relates to a functional feed added with a bloom-forming cyanobacteria extract.
Background
The water-blooming cyanobacteria is formed by gathering various cyanobacteria of microcystis, anabaena, nostoc and the like, wherein microcystis aeruginosa, anabaena water-blooming cyanobacteria and nostoc plankton are taken as main components, and the water-blooming cyanobacteria can rapidly propagate in a large amount in a fresh water environment, can destroy the ecological environment of a water body and cause huge economic loss.
It has been proved that microcystis aeruginosa, anabaena flos-aquae, nostoc plankton and the like in the bloom-forming cyanobacteria have anti-infection effects with different degrees, and the bacteriostasis rate of the mixed algae is stronger than that of single algae. Therefore, the water bloom blue algae is a new resource for screening and developing new antibiotics, and provides a new way for preventing and treating the pollution of the water bloom blue algae and improving the water ecological environment. In addition, the nature and the function of the algae in the bloom-forming cyanobacteria are different due to the difference of population structure and quantity composition.
Because of the effective bacteriostatic property of the bloom-forming cyanobacteria, the bloom-forming cyanobacteria is used as a bactericidal additive and has a plurality of applications in the fields of feeds, waste materials, biological medicines and the like.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide the functional feed added with the bloom-forming cyanobacteria extract.
In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:
a functional feed added with a water bloom blue algae extract comprises the following components in parts by weight:
wherein the waterweed powder is Taihu lake waterweed powder, and the waterweed is fished and collected from Taihu lake, cleaned, aired, dried, dehydrated and crushed into powder;
the preparation method of the water bloom blue algae extract comprises the following steps:
screening the bloom-forming cyanobacteria, filtering and dehydrating the bloom-forming cyanobacteria without large particle impurities to obtain dehydrated bloom-forming cyanobacteria, drying the dehydrated bloom-forming cyanobacteria, drying and crushing to obtain bloom-forming cyanobacteria powder;
adding water bloom cyanobacteria powder into a phosphate buffer solution, wherein the mass ratio of the water bloom cyanobacteria powder to the phosphate buffer solution is 2:25, then adding a complex enzyme, wherein the complex enzyme consists of papain, cellulase and pectinase, and inactivating the complex enzyme after the complex enzyme is subjected to enzymolysis to obtain an enzymolysis solution;
placing the enzymatic hydrolysate in a high-speed homogenizer, homogenizing for 3min, standing the homogenized solution at 2-4 deg.C for not less than 12 hr;
centrifuging at the rotating speed of 6000 rpm-;
dissolving the precipitate with sufficient distilled water, desalting by ultrafiltration, and dialyzing by ultrafiltration membrane to obtain supernatant and precipitate for the third time;
and mixing and centrifuging the second supernatant and the third supernatant to obtain a retention solution, adjusting the pH of the retention solution to be neutral, and freeze-drying to obtain the water-blooming cyanobacteria extract.
In a preferred embodiment of the present invention, the method further comprises drying and pulverizing the cyanobacteria bloom to obtain cyanobacteria bloom powder, and culturing the cyanobacteria bloom powder in a culture solution, wherein the culture solution comprises: ferric citrate, molybdic acid, glucose, potassium sulfate and potassium carbonate, wherein the concentration of a culture solution is 120mg/L, the pH of the culture solution is 7, and the water bloom cyanobacteria powder is cultured in the culture solution for 5 days to complete the induction of the chlamydospores;
the water bloom cyanobacteria powder is stirred for one time in a sterilization environment in the process of thick-wall spore induction, after 5 days, the water bloom cyanobacteria subjected to thick-wall spore induction is placed in an oven at the temperature of 30-40 ℃ to be dried, and the water bloom cyanobacteria powder subjected to thick-wall spore induction is used in the process of obtaining the water bloom cyanobacteria extract by enzymolysis.
In a preferred embodiment of the invention, the activity of three enzymes of papain, cellulase and pectinase in the complex enzyme is 100000 units.
In a preferred embodiment of the invention, the pH value is 5-9 when the compound enzyme is used for enzymolysis, and the temperature is 45-65 ℃ when the compound enzyme is used for enzymolysis.
In a preferred embodiment of the present invention, further comprising the supplementary feed component comprising: fish protein active polypeptide, pumpkin seed powder, chinaberry, folium artemisiae argyi and houttuynia cordata.
In a preferred embodiment of the present invention, further comprising, the vitamins include: vitamin A and vitamin B6Vitamin B12Vitamin B2Vitamin D, vitamin E.
The invention has the beneficial effects that:
the functional feed disclosed by the invention has the advantages that the water-blooming cyanobacteria extract is added in the feed formula, the water-blooming cyanobacteria extract is used as an additive, the escherichia coli, pseudomonas aeruginosa and staphylococcus aureus can be effectively killed, the sterilization rate is over 90%, the extract obtained after the water-blooming cyanobacteria is subjected to enzymolysis contains phycobiliprotein, and the water-blooming cyanobacteria extract has good cell oxidation resistance and immunity enhancing functions after being added in the feed through experimental verification.
The functional feed disclosed by the invention is added with the aquatic weed powder, and the aquatic weed powder contains more than 14% of protein, so that the soybean powder can be replaced, and the cost is reduced.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood and to be implemented in accordance with the content of the description, the specific embodiments of the present invention are given in detail by the following examples.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
In the present example, a functional feed is disclosed, the feed formulation is shown in table 1.
Table 1 formulation table in example 1
| Components | Content (parts by weight) |
| Bran | 50 |
| Corn flour | 35 |
| Aquatic weed powder | 15 |
| Water bloom blue algae extract | 3 |
| Yellow wine lees | 5 |
| Fish meal | 10 |
| Auxiliary feed component | 20 |
| Vitamin preparation | 2 |
Example 2
In example 2 a functional feed is disclosed, the feed formulation is shown in table 2.
Table 2 formulation table in example 2
| Components | Content (parts by weight) |
| Bran | 65 |
| Corn flour | 45 |
| Aquatic weed powder | 20 |
| Water bloom blue algae extract | 6 |
| Yellow wine lees | 10 |
| Fish meal | 15 |
| Auxiliary feed component | 22 |
| Vitamin preparation | 3 |
Example 3
A functional feed is disclosed in example 3, with the feed formulation shown in table 3.
Table 3 formula table in example 3
| Components | Content (parts by weight) |
| Bran | 80 |
| Corn flour | 55 |
| Aquatic weed powder | 30 |
| Water bloom blue algae extract | 9 |
| Yellow wine lees | 15 |
| Fish meal | 20 |
| Auxiliary feed component | 25 |
| Vitamin preparation | 5 |
In examples 1 to 3, wherein the waterweed powder is lake Tai waterweed powder, the waterweed is collected by fishing from lake Tai, and then cleaned, aired, dried, dehydrated and crushed into powder.
The auxiliary feed comprises the following components: fish protein active polypeptide, pumpkin seed powder, chinaberry, folium artemisiae argyi and houttuynia cordata.
The vitamins include: vitamin A and vitamin B6Vitamin B12Vitamin B2Vitamin D, vitamin E.
The preparation method of the water bloom blue algae extract comprises the following steps:
the first step is as follows: screening the bloom-forming cyanobacteria, filtering and dehydrating the bloom-forming cyanobacteria without large particle impurities to obtain dehydrated bloom-forming cyanobacteria, drying the dehydrated bloom-forming cyanobacteria, drying and crushing to obtain bloom-forming cyanobacteria powder;
the second step is that: adding water bloom cyanobacteria powder into a phosphate buffer solution, wherein the mass ratio of the water bloom cyanobacteria powder to the phosphate buffer solution is 2:25, then adding a complex enzyme, wherein the complex enzyme consists of papain, cellulase and pectinase, the activities of the papain, the cellulase and the pectinase are respectively 100000 units, the pH value of the complex enzyme during enzymolysis is 5-9, the temperature during enzymolysis is 45-65 ℃, and after the complex enzyme is subjected to enzymolysis, inactivating to obtain an enzymolysis solution;
the third step: placing the enzymatic hydrolysate in a high-speed homogenizer, homogenizing for 3min, standing the homogenized solution at 2-4 deg.C for not less than 12 hr;
the fourth step: centrifuging at the rotating speed of 6000 rpm-;
the fifth step: dissolving the precipitate with sufficient distilled water, desalting by ultrafiltration, and dialyzing by ultrafiltration membrane to obtain supernatant and precipitate for the third time;
and a sixth step: and mixing and centrifuging the second supernatant and the third supernatant to obtain a retention solution, adjusting the pH of the retention solution to be neutral, and freeze-drying to obtain the water-blooming cyanobacteria extract.
In order to prevent the bloom-forming cyanobacteria from mildewing and not easy to store, the bloom-forming cyanobacteria powder can be subjected to chlamydospore induction, and the specific method comprises the following steps: drying and crushing the bloom-forming cyanobacteria to obtain bloom-forming cyanobacteria powder, and then putting the bloom-forming cyanobacteria powder into a culture solution for culture, wherein the culture solution comprises: ferric citrate, molybdic acid, glucose, potassium sulfate and potassium carbonate, wherein the concentration of a culture solution is 120mg/L, the pH of the culture solution is 7, and the water bloom cyanobacteria powder is cultured in the culture solution for 5 days to complete the induction of the chlamydospores;
the water bloom cyanobacteria powder is stirred for one time in a sterilization environment in the process of thick-wall spore induction, after 5 days, the water bloom cyanobacteria subjected to thick-wall spore induction is placed in an oven at the temperature of 30-40 ℃ to be dried, and the water bloom cyanobacteria powder subjected to thick-wall spore induction is used in the process of obtaining the water bloom cyanobacteria extract by enzymolysis.
Comparative example 1
The formulation of comparative example 1 was compared to that of example 2, and no extract of bloom-forming cyanobacteria was added to the feed. The formulation is shown in table 4.
Table 4 formula of comparative example 1
Performance testing
1. Sterilizing property
1.1 preparing bacterial liquid, namely preparing escherichia coli bacterial liquid, pseudomonas aeruginosa bacterial liquid and staphylococcus aureus bacterial liquid respectively, wherein the flora is 50 cfu/L.
1.2 respectively placing the bacterial liquid in 50mL broth culture medium, and performing microscopic examination on the bacterial count;
1.3 dropping 5ml of the blue algae extract for water bloom obtained in the above example, culturing at 37 ℃ for 18 hours, and examining the number of bacteria under microscope, the results are shown in Table 5.
The bacteriostasis rate is (initial bacteria number-initial bacteria number)/initial bacteria number
TABLE 5 bacteriostatic ratio test result table
| Bacterial strain | Initial bacteria count (microscopic examination) | Number of viable bacteria (microscopic examination) | Bacteriostatic ratio (%) |
| Escherichia coli | 3103 | 270 | 91.3 |
| Pseudomonas aeruginosa | 5000 | 190 | 96.2 |
| Staphylococcus aureus | 2882 | 320 | 88.9 |
As can be seen from the results in Table 5, the blue algae extract obtained in the above examples has good bactericidal properties, wherein Escherichia coli is as high as 91.3%, Pseudomonas aeruginosa is as high as 96.2%, and Staphylococcus aureus is as high as 88.9%.
2. Determination of feed functionality
Four groups of male mice were used as experimental samples, 12 mice per group were numbered 1, 2, 3, 4, 1 group was fed with the feed of example 1, 2 groups was fed with the feed of example 2, 3 groups was fed with the feed of example 3, 4 groups was fed with the feed of comparative example 1, and the other feeding conditions were identical for the four groups of mice, and the mice were continuously fed for 60 days.
Four groups of mice were then infected with animal influenza a virus, of which 1 group had one mouse with symptoms of influenza within 24 hours, 2 mice had symptoms of slight influenza within 36 hours, and the mice healed 7 days later.
No symptoms of influenza appeared in group 2.
In 3 groups 1 mouse developed symptoms of influenza within 36 hours and healed 7 days later.
In 4 groups 4 mice developed flu symptoms within 24 hours, 2 mice developed flu symptoms within 36 hours, one of the mice developed flu symptoms within 24 hours developed pneumonia symptoms after 3 days, and 3 mice died after 7 days.
The experiment proves that the functional feed added with the water bloom blue algae extract has good immunity enhancing function.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. A functional feed added with a water bloom blue algae extract is characterized by comprising the following components in parts by weight:
50-80 parts of bran
35-55 parts of corn flour
15-30 parts of water plant powder
3-9 parts of water bloom blue algae extract
5-15 parts of yellow wine lees
10-20 parts of fish meal
20-25 parts of auxiliary feed component
2-5 parts of vitamin
Wherein the waterweed powder is Taihu lake waterweed powder, and the waterweed is fished and collected from Taihu lake, cleaned, aired, dried, dehydrated and crushed into powder;
the preparation method of the water bloom blue algae extract comprises the following steps:
screening the bloom-forming cyanobacteria, filtering and dehydrating the bloom-forming cyanobacteria without large particle impurities to obtain dehydrated bloom-forming cyanobacteria, drying the dehydrated bloom-forming cyanobacteria, drying and crushing to obtain bloom-forming cyanobacteria powder;
adding water bloom cyanobacteria powder into a phosphate buffer solution, wherein the mass ratio of the water bloom cyanobacteria powder to the phosphate buffer solution is 2:25, then adding a complex enzyme, wherein the complex enzyme consists of papain, cellulase and pectinase, and inactivating the complex enzyme after the complex enzyme is subjected to enzymolysis to obtain an enzymolysis solution;
placing the enzymatic hydrolysate in a high-speed homogenizer, homogenizing for 3min, standing the homogenized solution at 2-4 deg.C for not less than 12 hr;
centrifuging at the rotating speed of 6000 rpm-;
dissolving the precipitate with sufficient distilled water, desalting by ultrafiltration, and dialyzing by ultrafiltration membrane to obtain supernatant and precipitate for the third time;
mixing the second supernatant and the third supernatant, centrifuging to obtain a retention solution, adjusting the pH of the retention solution to be neutral, and freeze-drying to obtain a bloom blue algae extract;
wherein, after the water bloom blue algae is dried and crushed to obtain water bloom blue algae powder, the water bloom blue algae powder is put into a culture solution for culture, and the culture solution comprises: ferric citrate, molybdic acid, glucose, potassium sulfate and potassium carbonate, wherein the concentration of a culture solution is 120mg/L, the pH of the culture solution is 7, and the water bloom cyanobacteria powder is cultured in the culture solution for 5 days to complete the induction of the chlamydospores;
the water bloom cyanobacteria powder is stirred for one time in a sterilization environment in the process of thick-wall spore induction, after 5 days, the water bloom cyanobacteria subjected to thick-wall spore induction is placed in an oven at the temperature of 30-40 ℃ to be dried, and the water bloom cyanobacteria powder subjected to thick-wall spore induction is used in the process of obtaining the water bloom cyanobacteria extract by enzymolysis.
2. The functional feed added with the water-blooming cyanobacteria extract as claimed in claim 1, wherein the activities of three enzymes, namely papain, cellulase and pectinase in the complex enzyme are 100000 units each.
3. The functional feed added with the water-blooming cyanobacteria extract as claimed in claim 1, wherein the pH value is 5-9 when the compound enzyme is used for enzymolysis, and the temperature is 45-65 ℃ when the compound enzyme is used for enzymolysis.
4. The functional feed added with the extract of the blue algae for water bloom according to claim 1, wherein the auxiliary feed components comprise: fish protein active polypeptide, pumpkin seed powder, chinaberry, Chinese mugwort leaf and houttuynia cordata.
5. The functional feed added with the extract of the blue algae for water bloom according to claim 1, wherein the vitamins comprise: vitamin A, vitamin B6Vitamin B12Vitamin B2Vitamin D, vitamin E.
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| WO2022110088A1 (en) * | 2020-11-28 | 2022-06-02 | 南京溧水高新创业投资管理有限公司 | Culture medium for screening high-yield gibberellin strain |
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