CN105211551A - A kind of high-efficiency biological active fodder additives for ruminant and preparation method thereof - Google Patents
A kind of high-efficiency biological active fodder additives for ruminant and preparation method thereof Download PDFInfo
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Abstract
The invention discloses the high-efficiency biological active fodder additives for ruminant and preparation method: prepare compound Chinese herb fermentation dry powder; Prepare nostoc fermentate dry powder: get 20-26 part compound Chinese herb fermentation dry powder, 1-1.6 part nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix both finished product.High-efficiency biological active fodder additives of the present invention is drawn materials natural without any side effects, not only can strengthen the physique of animal, immunity, stress ability, and noresidue, harmless.High-efficiency biological active fodder additives of the present invention, it is for ruminant, containing multiple Chinese herbal medicine effective ingredients, active bacteria, enzyme etc.And the present invention also novelty add a small amount of nanomete talc powder, can greatly extend the shelf life, under normal temperature, storage can reach 24 and active high, only needs to add 0.06-0.1% and can reach very good effect.
Description
Technical field
The present invention relates to a kind of high-efficiency biological active fodder additives for ruminant and preparation method thereof, belong to feed additive field.
Background technology
Feed addictive refers at Feed Manufacturing processing, a small amount of or micro substance that adds in use procedure, in feed consumption seldom but effect significantly.Feed addictive is the raw material that modern feed industry must use, and to strengthening basal feed nutritive value, improve breeding performonce fo animals, ensure animal health, save feed cost, improving the aspects such as livestock products quality has obvious effect.
Along with the enhancing of health of people consciousness, requirement is also improved to the quality of various animal products and security, therefore, while improving food conversion ratio and livestock and poultry grow fast, develop the green feed additive of alternative antibiotic and chemical synthetic drug, thus ensure animal products safety, become the problem that present feed industry development will solve.
Ruminate (ch ú) to refer to that half food digested under one's belt returns by feed through after a while after and again chew in the mouth.Ruminant is exactly the animal ruminating phenomenon, normally some herbivores, because the fiber of plant is more heavy.Ruminant searches for food general relatively more hasty, particularly roughage, and major part enters cud without fully chewing just to swallow, through rumen bypass immersion with after softening a period of time, food comes back to oral cavity through inverse vomitting, and through chewing again, being again mixed into saliva and swallowing the process entering cud again.Ruminate (ch ú) to refer to that half food digested under one's belt returns by feed through after a while after and again chew in the mouth.Ruminant is exactly the animal ruminating phenomenon, normally some herbivores, because the fiber of plant is more heavy.Ruminant searches for food general relatively more hasty, particularly roughage, and major part enters cud without fully chewing just to swallow, through rumen bypass immersion with after softening a period of time, food comes back to oral cavity through inverse vomitting, and through chewing again, being again mixed into saliva and swallowing the process entering cud again.Except the digestion of microorganism and digestive ferment, ruminant has been assisted the contraction of muscle motion that the digestion of food also relies on these four bodies of stomach.The effect of three aspects is mainly played in the muscular movement of coat of the stomach: storage food function, mixed food and gastric juice are to form semi-fluid chyme function and emptying food function.
There is multiple compound Chinese herbal medicinal feed additive in prior art, additive for microbe feedstuff, or even Chinese medicine high-efficiency biological active fodder additives.Chinese medicine high-efficiency biological active fodder additives contains a large amount of beneficial bacteriums (biodiasmin, Bifidobacterium, bacillus), complex enzyme, chelating peptide, enters after in livestock and poultry body as feed by de-mould dose etc., can breed rapidly, drop in the metabolin of bacterial classification on the one hand and enterogenous endotoxin, inhibit the growth of other harmful flora, in host, define normal microbial flora on the other hand, for host synthesizes main vitamin, provide the invasion of nutrition and prevention pathogenic bacteria.
The present invention aims to provide a kind of high-efficiency biological active fodder additives for ruminant and preparation method thereof, and its source is natural, active high, and only need to add 0.06-0.1%, applicability is wide, may be used for multiple common ruminant, comprises ox, the animals such as sheep.It can efficient hardening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, saves feed cost, has obvious effect in many-sides such as improving livestock products quality.
Summary of the invention
Based on the technical problem that background technology exists, the present invention is directed to background technology Problems existing, a kind of high-efficiency biological active fodder additives for ruminant and preparation method thereof is provided, its source is natural, active high, only needs to add 0.06-0.1%, applicability is wide, may be used for multiple common ruminant, comprise ox, the animals such as sheep.It can efficient hardening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, saves feed cost, has obvious effect in many-sides such as improving livestock products quality.
Object of the present invention is achieved through the following technical solutions:
A kind of preparation method of the high-efficiency biological active fodder additives for ruminant:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 2-3 part trehalose, 0.2 part of shitosan, 1-1.2 part tourmaline powder, 15-16 part folium cortex eucommiae, 5-6 part Fructus Hordei Germinatus, 2-3 part cordate houttuynia, 0.5-0.8 part Radix Astragali, 1-1.5 part Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, add 8 parts of water furnishing pasty states again, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 50-55 part nostoc, cleaning, wears into meal with grinder, then uses 90-95 DEG C of hot-water soak 50-85min, pull out after draining, add 10-12 part normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1-1.5%, keep temperature 38 DEG C, fermentation 52-58 hour, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio bacillus subtilis: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 20-26 part compound Chinese herb fermentation dry powder, 1-1.6 part nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix both finished product.
Described enzymatic activity data are:
'beta '-mannase: 20000-25000U/kg, zytase: 1.2 × 10
6u/kg; 1,4 beta-glucanase: 6.7 × 10
5u/kg, pectase: 6.0 × 10
5u/kg; Cellulase: 9.0 × 10
6u/kg
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
The compatibility of Chinese herbal feed additive both can select simple, also the compound that multi-flavor medicine forms can be selected, but not the simple rough combination of some medicines, without " method " nothing " side " nothing " compatibility ", but under the guidance of Chinese veterinarian's theory, according to inherent laws and the feature thereof of Chinese veterinarian's pharmacy, on theory contact practical basis, consider that compatibility of drugs gets up to use by the performance of modern medicines itself selectively, give full play to the mass action of medicine.Medicine for the concrete condition of animal, according to certain principle, should combine, just defines prescription by the prescription of Chinese herbal medicine selectively.This principle is said in " Plain Questions to very will discuss greatly ": " main disease be monarch, what help monarch is minister, answer minister for making.Monarch one minister two, system also little; Monarch one minister three helps five, among system also; Monarch one minister three helps nine, makes also big." principle of Here it is herbal prescription.The principles of formulating prescriptions of Chinese herbal medicine may be summarized to be monarch, minister, help, make, be referred to as now more main and auxiliary, help, make four aspects.According to characteristic and the major function of various Chinese herbal medicine, adopt extraction, its active component of concentrated natural plant, screening is optimization of C/C composites also, improve and optimize traditional Chinese herbal and crude drugs preparations technique, develop and can strengthen domestic animal immunologic function, improve its survival rate, promote that it grows, there is the compound Chinese herbal medicinal feed additive of the effects such as well antiviral, sterilization, desinsection.
Nostoc (latin name: Pogostemonauricularius (L.) Kassk; Formal name used at school: intend spherical nostoc) be commonly called as beauty's rice, sky rosetangle, loosestrife, water auricularia auriculajudae, field auricularia auriculajudae.Nostoc Cyanophyceae, nostocaceae.Frond is colloidal substance, spherical or other are irregularly shaped, blue-green or yellowish-brown.Cell is spherical, is linked to be beads shape colony, outsourcing colloid by most cell.Heterocyst is positioned at the iuntercellular of filament, and young filament is often positioned at top.Most generation akinete during breeding, often for concatenating into chain.In green time moistening, in grey black after dry.Between attached submersed sandstone or on dark and damp earth.All have distribution all over China, with Sichuan produce the most famous.Edible.There is nitrogen fixing capacity.Several amino acids containing needed by human and polysaccharide isoreactivity material.Have relieve inflammation or internal heat, improving eyesight, the therapeutic efficiency such as anti-ageing, anti-infective.
The present invention is on the basis of Chinese traditional herbs theory, in conjunction with modernization bio-fermentation process, prepare a kind of high-efficiency biological active fodder additives for ruminant and preparation method thereof, its source is natural, active high, only needs to add 0.06-0.1%, applicability is wide, may be used for multiple common ruminant, comprise ox, the animals such as sheep.It can efficient hardening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, saves feed cost, has obvious effect in many-sides such as improving livestock products quality.And the present invention is and novelty adds a small amount of nanomete talc powder, can greatly extend the shelf life, under normal temperature, storage can reach 24 months.
Usefulness of the present invention is:
1) high-efficiency biological active fodder additives of the present invention is drawn materials natural without any side effects, not only can strengthen the physique of animal, immunity, stress ability, and noresidue, harmless.
2) high-efficiency biological active fodder additives of the present invention, it is for ruminant, containing multiple Chinese herbal medicine effective ingredients, active bacteria, enzyme etc.And the present invention is and novelty adds a small amount of nanomete talc powder, can greatly extend the shelf life, under normal temperature, storage can reach 24 months.
3) composite highly effective bioactivity feed additive applicability of the present invention is wide, active high, is applicable to multiple ruminant, only needs to add 0.06-0.1% and can reach very good effect.
Detailed description of the invention
The present invention's enzymatic activity data used are:
'beta '-mannase: 20000-25000U/kg, zytase: 1.2 × 10
6u/kg; 1,4 beta-glucanase: 6.7 × 10
5u/kg, pectase: 6.0 × 10
5u/kg; Cellulase: 9.0 × 10
6u/kg
Embodiment 1:
Preparation for the high-efficiency biological active fodder additives of ruminant:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 2 portions of trehaloses, 0.2 part of shitosan, 1.2 parts of tourmaline powders, 15 parts of folium cortex eucommiaes, 6 parts of Fructus Hordei Germinatus, 2 portions of cordate houttuynias, 0.8 part of Radix Astragali, 1 part of Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 8 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 55 portions of nostoc, cleaning, wears into meal with grinder, then uses 90 DEG C of hot-water soak 85min, pull out after draining, add 10 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.5%, keep temperature 38 DEG C, ferment 52 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio bacillus subtilis: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 26 parts of compound Chinese herb fermentation dry powders, 1 part of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Add a small amount of talcum powder can greatly extend the shelf life, preserve 24 months under normal temperature, its number of viable and nutritional cost do not change substantially.
Embodiment 2:
Preparation for the high-efficiency biological active fodder additives of ruminant:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 3 portions of trehaloses, 0.2 part of shitosan, 1 part of tourmaline powder, 16 parts of folium cortex eucommiaes, 5 parts of Fructus Hordei Germinatus, 3 portions of cordate houttuynias, 0.5 part of Radix Astragali, 1.5 parts of Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 8 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 50 portions of nostoc, cleaning, wears into meal with grinder, then uses 95 DEG C of hot-water soak 50min, pull out after draining, add 12 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1%, keep temperature 38 DEG C, ferment 58 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio bacillus subtilis: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 20 parts of compound Chinese herb fermentation dry powders, 1.6 parts of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Add a small amount of talcum powder can greatly extend the shelf life, preserve 24 months under normal temperature, its number of viable and nutritional cost do not change substantially.
Embodiment 3:
Preparation for the high-efficiency biological active fodder additives of ruminant:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 2 portions of trehaloses, 0.2 part of shitosan, 1.1 parts of tourmaline powders, 15 parts of folium cortex eucommiaes, 6 parts of Fructus Hordei Germinatus, 2 portions of cordate houttuynias, 0.7 part of Radix Astragali, 1.2 parts of Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 8 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 54 portions of nostoc, cleaning, wears into meal with grinder, then uses 91 DEG C of hot-water soak 70min, pull out after draining, add 11 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.4%, keep temperature 38 DEG C, ferment 53 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio bacillus subtilis: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 25 parts of compound Chinese herb fermentation dry powders, 1.2 parts of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Add a small amount of talcum powder can greatly extend the shelf life, preserve 24 months under normal temperature, its number of viable and nutritional cost do not change substantially.
Embodiment 4:
Preparation for the high-efficiency biological active fodder additives of ruminant:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 3 portions of trehaloses, 0.2 part of shitosan, 1.2 parts of tourmaline powders, 16 parts of folium cortex eucommiaes, 5 parts of Fructus Hordei Germinatus, 3 portions of cordate houttuynias, 0.6 part of Radix Astragali, 1.4 parts of Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 8 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 51 portions of nostoc, cleaning, wears into meal with grinder, then uses 94 DEG C of hot-water soak 60min, pull out after draining, add 10 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.2%, keep temperature 38 DEG C, ferment 57 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio bacillus subtilis: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 21 parts of compound Chinese herb fermentation dry powders, 1.5 parts of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Add a small amount of talcum powder can greatly extend the shelf life, preserve 24 months under normal temperature, its number of viable and nutritional cost do not change substantially.
Embodiment 5:
Preparation for the high-efficiency biological active fodder additives of ruminant:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 2 portions of trehaloses, 0.2 part of shitosan, 1 part of tourmaline powder, 15 parts of folium cortex eucommiaes, 6 parts of Fructus Hordei Germinatus, 2 portions of cordate houttuynias, 0.8 part of Radix Astragali, 1.3 parts of Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 8 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 53 portions of nostoc, cleaning, wears into meal with grinder, then uses 92 DEG C of hot-water soak 80min, pull out after draining, add 12 parts of normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1.3%, keep temperature 38 DEG C, ferment 54 hours, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio bacillus subtilis: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 24 parts of compound Chinese herb fermentation dry powders, 1.3 parts of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix and both obtained finished product.
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
Add a small amount of talcum powder can greatly extend the shelf life, preserve 24 months under normal temperature, its number of viable and nutritional cost do not change substantially.
Embodiment 6:
Nostoc does not carry out fermenting in contrast:
Preparation for the high-efficiency biological active fodder additives of ruminant:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 3 portions of trehaloses, 0.2 part of shitosan, 1.1 parts of tourmaline powders, 16 parts of folium cortex eucommiaes, 5 parts of Fructus Hordei Germinatus, 3 portions of cordate houttuynias, 0.5 part of Radix Astragali, 1.1 parts of Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, then add 8 parts of water furnishing pasty states, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc dry powder is prepared:
Get nostoc, cleaning, dry, grind to form fine powder;
(3) get 22 parts of compound Chinese herb fermentation dry powders, 1.4 parts of nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix and both obtained finished product.
Embodiment 7 milking cow feeding effect is tested and assessed:
Choose 350 milk cow in lactation period according to the principle of " kind is identical, body weight is close ", be divided into 7 groups at random, often group establishes 5 repetitions, each repetition 10 cow head.
Control group is Conventional antibiotic control group, and other groups add the biology feed additive of the present invention of 0.1% respectively in basal diet.
Every leading indicator statistical analysis group difference of each test group milk cow is not remarkable.
Feeding manner adopts group feeding, and every day, feeding 2 times, freely drank water.Other routinely management method carry out.Experimental period is 30d.
Result is as shown in table 1 below.
Table 1
To dairy products attribute test, result is as shown in table 2:
Table 2
| Butterfat percnetage (%) | Lactoprotein ratio (%) | Real milk solids ratio (%) | |
| Control group | 6.8 | 3.5 | 18.1 |
| Embodiment 6 | 7.2 | 3.6 | 18.5 |
| Embodiment 1 | 8.8 | 5.1 | 18.9 |
| Embodiment 2 | 8.5 | 4.8 | 19.0 |
| Embodiment 3 | 8.3 | 5.3 | 19.1 |
| Embodiment 4 | 8.7 | 5.5 | 18.9 |
| Embodiment 5 | 8.4 | 4.6 | 19.1 |
Repeat above-mentioned experiment, difference is only the biology feed additive of the present invention adding 0.06% in basal diet.
Result is as shown in table 3 below.
Table 3
As can be seen here, the high-efficiency biological active fodder additives of the present invention adding minimum 0.06% in basal diet can to the condition of milk cow, and dairy food quality plays good raising effect.
Embodiment 8 tremble with fear sheep feeding effect test and appraisal:
That selected for 4 monthly ages fattens cold sheep 350, is divided into 7 groups at random, and often group establishes 5 repetitions, each repetition 10 bellwether, and every sheep body weight difference is no more than 1.5 kilograms, and feed 3 months, equal free choice feeding, fully drinks water.
Tremble with fear every leading indicator statistical analysis group difference of sheep of each test group is not remarkable.
Control group is Conventional antibiotic control group, and other groups add the biology feed additive of the present invention of 0.06% respectively in basal diet.
Feeding experiment result is as shown in table 4:
Table 4
Visible, the high-efficiency biological active fodder additives of the present invention adding minimum 0.06% in basal diet can improve the condition of cold sheep, reduces feedstuff-meat ratio.
In addition, select 350 female cold sheep of gestation, be divided into 7 groups at random, often group establishes 5 repetitions, each repetition 10 bellwether, and control group is Conventional antibiotic control group, and other groups add the biology feed additive of the present invention of 0.06% respectively in basal diet.Other rearing conditions are all identical, and carry out separated raising during pregnancy ewes childbirth, equal free choice feeding, fully drinks water.The pregnancy ewes pilot production phase is 60d, and namely duration of test institute lambing sheep is litter size, and during 60d wean, the survival number of lamb is for surviving number, and duration of test is observed and recorded the situations such as the rickets of lamb, allotriophagy, anaemia and death in detail.
Result is as shown in table 5:
Table 5
| Lamb survival rate (%) | Anaemia lamb (%) | Rickets lamb (%) | Allotriophagy lamb (%) |
| Embodiment 1 | 100 | 0.8 | 1.3 | 0 |
| Embodiment 2 | 94.7 | 0 | 0 | 0 |
| Embodiment 3 | 98.5 | 0 | 0 | 0 |
| Embodiment 4 | 93.1 | 0 | 0 | 0 |
| Embodiment 5 | 96.9 | 0 | 0 | 1.5 |
| Embodiment 6 | 81.2 | 3.5 | 4.2 | 4.9 |
| Control group | 71.6 | 31.5 | 19.6 | 29.8 |
As can be seen here, the biology feed additive of the present invention adding 0.06% in basal diet can play good facilitation to the growth of cold sheep, the physique of particularly increase significantly to the female cold sheep of gestation lamb survival rate and lamb.
Visible, the high-efficiency biological active fodder additives for ruminant of the present invention is drawn materials natural without any side effects, not only can strengthen the physique of animal, immunity, stress ability, and noresidue, harmless.It is for ruminant, containing multiple Chinese herbal medicine effective ingredients, and active bacteria, enzyme etc.And the present invention is and novelty adds a small amount of nanomete talc powder, can greatly extend the shelf life, under normal temperature, storage can reach 24 months.And composite highly effective bioactivity feed additive applicability of the present invention is wide, active high, be applicable to multiple ruminant, only need to add 0.06-0.1% and can reach very good effect.
The above; be only the present invention's preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (6)
1., for a preparation method for the high-efficiency biological active fodder additives of ruminant, its step is as follows:
As described belowly be mass parts;
(1) compound Chinese herb fermentation dry powder is prepared:
Get 2-3 part trehalose, 0.2 part of shitosan, 1-1.2 part tourmaline powder, 15-16 part folium cortex eucommiae, 5-6 part Fructus Hordei Germinatus, 2-3 part cordate houttuynia, 0.5-0.8 part Radix Astragali, 1-1.5 part Divine Comedy, fully mix after grinding to form fine powder, continue to add 0.2 part of feed grade laccase, mixing, add 8 parts of water furnishing pasty states again, after placing 20min at normal temperatures, then according to the ratio microbe inoculation leavening of inoculum concentration 0.5%, keep temperature 38 DEG C, ferment 20 hours, low temperature drying after fermentation ends, grind to form fine powder, be Chinese herbal medicine fermentation dry powder;
The composition of described microbe leaven is: according to bacterial strain number ratio Bacillus acidi lactici: bacillus coagulans: yellow Ruminococcus is: the proportions of 1:1.8:1.2 becomes 1.8*10
7the leavening of bacterial strain/ml;
(2) nostoc fermentate dry powder is prepared:
Get 50-55 part nostoc, cleaning, wears into meal with grinder, then uses 90-95 DEG C of hot-water soak 50-85min, pull out after draining, add 10-12 part normal temperature pure water, and even, then according to the ratio microbe inoculation leavening of inoculum concentration 1-1.5%, keep temperature 38 DEG C, fermentation 52-58 hour, low temperature drying after fermentation ends, grinds to form fine powder;
The composition of described microbe leaven is: according to bacterial strain number ratio bacillus subtilis: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:1.2:2 becomes 1.5*10
7the leavening of bacterial strain/ml;
(3) get 20-26 part compound Chinese herb fermentation dry powder, 1-1.6 part nostoc fermentate dry powder, 0.1 part of nanomete talc powder, 0.05 part of 'beta '-mannase, 0.1 part of 1,4 beta-glucanase, 0.1 part of cellulase, 0.08 part of pectase, 0.05 part of zytase fully mix both finished product.
2. the preparation method of the high-efficiency biological active fodder additives for ruminant according to claim 1, is characterized in that:
Described low temperature drying is control temperature is less than 40 DEG C dryings, to reduce the infringement to microorganism.
3. the high-efficiency biological active fodder additives for ruminant for preparing of the preparation method of the described high-efficiency biological active fodder additives for ruminant of claim 1-2.
4. the high-efficiency biological active fodder additives for ruminant according to claim 3 is used for ox of feeding.
5. composite highly effective bioactivity feed additive according to claim 3 is for the application of sheep of feeding.
6. the application described in claim 4-5, is characterized in that:
Its addition is 0.06-0.1%.
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