CN107047972A - A kind of functional feed for adding bloom blue algae extract - Google Patents
A kind of functional feed for adding bloom blue algae extract Download PDFInfo
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- CN107047972A CN107047972A CN201710270536.9A CN201710270536A CN107047972A CN 107047972 A CN107047972 A CN 107047972A CN 201710270536 A CN201710270536 A CN 201710270536A CN 107047972 A CN107047972 A CN 107047972A
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- blue algae
- bloom blue
- powder
- bloom
- algae extract
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses it is a kind of add bloom blue algae extract functional feed, in parts by weight, including：50 80 parts of wheat bran, 35 55 parts of corn flour, 15 30 parts of pasture and water powder, 39 parts of bloom blue algae extract, 5 15 parts of yellow wine lees, 10 20 parts of fish meal, 20 25 parts of auxiliary fodder component, 25 parts of vitamin.The present invention with the addition of bloom blue algae extract in feed formula, additive is used as using bloom blue algae extract, Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus can effectively be killed, and sterilizing rate is more than 90%, extract of the bloom blue algae after enzymolysis, containing phycobniliprotein, by verification experimental verification, added in feed, with the good enhanced function of cellular anti-oxidant and immunity.
Description
Technical field
The present invention relates to field of fodder, and in particular to a kind of functional feed of addition bloom blue algae extract.
Background technology
Bloom blue algae is formed by a variety of blue-green algae aggregations such as microcystis kutz, Anabaena, Nostoc, wherein again with verdigris
Based on Microcystis aeruginosa, Anabaena Flos-aquae, the nostoc that swims, bloom blue algae can in fresh water environment rapid amount reproduction, can break
Bad water ecological setting, causes huge economic losses.
Have confirmed that microcystic aeruginosa, Anabaena Flos-aquae, the nostoc that swims in bloom blue algae etc. have different journeys now
The anti-infectious function of degree, and the bacteriostasis rate of mixed algae is stronger than single algae.Therefore, bloom blue algae is screening and develops new anti-
The new resources of raw element, are also preventing and treating bloom blue algae pollution, improve water ecological environment and provide a new way.Also, due to water
The population structure of algae in Chinese blue algae, the difference of quantity composition, its property effect is different.
Because the effective bacteriostasis property of bloom blue algae, it is used as bactericidal additive, in feed, waste material, bio-pharmaceutical etc.
There are numerous applications in field.
The content of the invention
It is an object of the invention to overcome the problem above that prior art is present, there is provided one kind addition bloom blue algae extract
Functional feed, the present invention bloom blue algae extract is with the addition of in feed, feed has good bactericidal action.
To realize above-mentioned technical purpose and the technique effect, the present invention is achieved through the following technical solutions：
A kind of functional feed for adding bloom blue algae extract, in parts by weight, including：
Wherein, the pasture and water powder is TAIHU LAKE grass meal, is salvaged from Taihu Lake and collects pasture and water, and then cleaning, drying, drying are de-
Water, it is ground into powder；
The preparation method of the bloom blue algae extract includes：
Sieve takes bloom blue algae, then carries out filtering means dehydration to the bloom blue algae for removing large granular impurity, obtains being dehydrated wawter bloom
Blue-green algae, then dries the bloom blue algae of dehydration, and bloom blue algae powder is obtained after dries pulverizing；
Bloom blue algae powder is added in phosphate buffer, the mass ratio of bloom blue algae powder and phosphate buffer is 2:
25, complex enzyme is then added, complex enzyme is made up of papain, cellulase, pectase, after complex enzyme is digested, and is entered
Row inactivation, obtains enzymolysis liquid；
Enzymolysis liquid is put into high-shear homogenizer, homogeneous 3min, the solution after homogeneous is stood at 2-4 DEG C, stood not
Less than 12h；
6-8min is centrifuged under 4000-6000rpm rotating speeds, first time supernatant is obtained, first time supernatant is added into sulfuric acid
After ammonium or ammonium carbonate, standing, retain supernatant, continuously add ammonium sulfate or ammonium carbonate to saturation, 10000-12000rpm rotating speeds
Lower centrifugation 10min, after separation, obtains second of supernatant and precipitation；
Precipitation is dissolved with enough distilled water, using ultrafiltration desalination, milipore filter dialysis obtains third time supernatant and precipitation；
Second of supernatant and third time supernatant are mixed after centrifugation, obtains and retains liquid, retains liquid and adjusts pH to neutrality,
Then it is freeze-dried, obtains bloom blue algae extract.
In the preferred embodiment of the present invention, further comprise, bloom blue algae dries pulverizing is obtained into bloom blue algae
After powder, then bloom blue algae powder is put into nutrient solution cultivated, include in nutrient solution：Ironic citrate, molybdic acid, glucose, potassium sulfate,
Potassium carbonate, the concentration of nutrient solution is 120mg/L, and the pH of nutrient solution is 7, and bloom blue algae powder is cultivated 5 days in nutrient solution, completes thick
Wall Spore inducing；
Bloom blue algae powder is in akinete Induction Process, under sterilized environment, and timing agitation once, after 5 days, will pass through
Bloom blue algae after akinete induction, which is placed in 30-40 DEG C of baking oven, dries, and obtains the wawter bloom induced by akinete
Blue algae powder, the bloom blue algae powder after akinete induces is used during enzymolysis obtains bloom blue algae extract.
In the preferred embodiment of the present invention, further comprise, papain, cellulose in the complex enzyme
Enzyme, the vigor of three kinds of enzymes of pectase are respectively 100000 unit meters.
In the preferred embodiment of the present invention, further comprise, the use of the pH during complex enzyme zymohydrolysis be 5-9,
Temperature during enzymolysis is 45-65 DEG C.
In the preferred embodiment of the present invention, further comprise, the auxiliary fodder component includes：Fish protein activity
Polypeptide, pumpkin seed powder, China tree, folium artemisiae argyi, cordate houttuynia.
In the preferred embodiment of the present invention, further comprise, the vitamin includes：Vitamin A, vitamin
B6, vitamin B12, vitamin B2, vitamin D, vitamin E.
The beneficial effects of the invention are as follows:
The functional feed of the present invention, with the addition of bloom blue algae extract, with bloom blue algae extract in feed formula
As additive, Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus can be effectively killed, and sterilizing rate is more than 90%, water
Extract of the Chinese blue algae after enzymolysis, containing phycobniliprotein, by verification experimental verification, is added in feed, with good
Cellular anti-oxidant and the enhanced function of immunity.
The functional feed of the present invention with the addition of pasture and water powder, and pasture and water powder contains more than 14% protein, can replace beans
Powder, reduces cost.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, embodiment of the invention is shown in detail by following examples.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
Embodiment 1
A kind of functional feed is disclosed in the present embodiment, feed formula is as shown in table 1.
Formula table in the embodiment 1 of table 1
| Component | Content (parts by weight) |
| Wheat bran | 50 |
| Corn flour | 35 |
| Pasture and water powder | 15 |
| Bloom blue algae extract | 3 |
| Yellow wine lees | 5 |
| Fish meal | 10 |
| Auxiliary fodder component | 20 |
| Vitamin | 2 |
Embodiment 2
A kind of functional feed is disclosed in embodiment 2, feed formula is as shown in table 2.
Formula table in the embodiment 2 of table 2
| Component | Content (parts by weight) |
| Wheat bran | 65 |
| Corn flour | 45 |
| Pasture and water powder | 20 |
| Bloom blue algae extract | 6 |
| Yellow wine lees | 10 |
| Fish meal | 15 |
| Auxiliary fodder component | 22 |
| Vitamin | 3 |
Embodiment 3
A kind of functional feed is disclosed in embodiment 3, feed formula is as shown in table 3.
Formula table in the embodiment 3 of table 3
| Component | Content (parts by weight) |
| Wheat bran | 80 |
| Corn flour | 55 |
| Pasture and water powder | 30 |
| Bloom blue algae extract | 9 |
| Yellow wine lees | 15 |
| Fish meal | 20 |
| Auxiliary fodder component | 25 |
| Vitamin | 5 |
In embodiment 1-3, wherein, the pasture and water powder is TAIHU LAKE grass meal, is salvaged from Taihu Lake and collects pasture and water, Ran Houqing
Clean, drying, drying and dewatering, it is ground into powder.
The auxiliary fodder component includes：Fish protein active peptides, pumpkin seed powder, China tree, folium artemisiae argyi, cordate houttuynia.
The vitamin includes：Vitamin A, vitamin B6, vitamin B12, vitamin B2, vitamin D, vitamin E.
The preparation method of the bloom blue algae extract includes：
The first step：Sieve takes bloom blue algae, then carries out filtering means dehydration to the bloom blue algae for removing large granular impurity, is taken off
Water bloom blue algae, then dries the bloom blue algae of dehydration, and bloom blue algae powder is obtained after dries pulverizing；
Second step：Bloom blue algae powder is added in phosphate buffer, the quality of bloom blue algae powder and phosphate buffer
Than for 2:25, complex enzyme is then added, complex enzyme is made up of papain, cellulase, pectase, papain, fibre
The plain enzyme of dimension, the vigor of three kinds of enzymes of pectase are respectively 100000 unit meters, and pH during complex enzyme zymohydrolysis is 5-9, temperature during enzymolysis
For 45-65 DEG C, after complex enzyme is digested, inactivated, obtain enzymolysis liquid；
3rd step：Enzymolysis liquid is put into high-shear homogenizer, homogeneous 3min, the solution after homogeneous is stood at 2-4 DEG C,
Standing is not less than 12h；
4th step：6-8min is centrifuged under 4000-6000rpm rotating speeds, first time supernatant is obtained, first time supernatant is added
Enter after ammonium sulfate or ammonium carbonate, standing, retain supernatant, continuously add ammonium sulfate or ammonium carbonate to saturation, 10000-
10min is centrifuged under 12000rpm rotating speeds, after separation, second of supernatant and precipitation is obtained；
5th step：Precipitation is dissolved with enough distilled water, using ultrafiltration desalination, milipore filter dialysis obtains third time supernatant
And precipitation；
6th step：Second of supernatant and third time supernatant are mixed after centrifugation, obtains and retains liquid, retains liquid regulation pH
To neutral, then it is freeze-dried, obtains bloom blue algae extract.
In order to prevent bloom blue algae from going mouldy, storage is difficult, bloom blue algae powder can be carried out to akinete induction, specific side
Method is：Bloom blue algae dries pulverizing is obtained after bloom blue algae powder, then bloom blue algae powder is put into culture, nutrient solution in nutrient solution
In include：Ironic citrate, molybdic acid, glucose, potassium sulfate, potassium carbonate, the concentration of nutrient solution is 120mg/L, and the pH of nutrient solution is
7, bloom blue algae powder is cultivated 5 days in nutrient solution, completes akinete induction；
Bloom blue algae powder is in akinete Induction Process, under sterilized environment, and timing agitation once, after 5 days, will pass through
Bloom blue algae after akinete induction, which is placed in 30-40 DEG C of baking oven, dries, and obtains the wawter bloom induced by akinete
Blue algae powder, the bloom blue algae powder after akinete induces is used during enzymolysis obtains bloom blue algae extract.
Comparative example 1
Bloom blue algae extract is not added with formula in comparative example 1 and the contrast in embodiment 2, feed.Formula such as table 4
Shown in.
The formula table of the comparative example 1 of table 4
Performance test
1st, bactericidal properties
Escherichia coli bacteria liquid, Pseudomonas aeruginosa bacterium solution, staphylococcus aureus bacterium solution, flora is respectively configured in 1.1 configuration bacterium solutions
For 50cfu/L.
1.2 are respectively placed in above-mentioned bacterium solution in 50mL broth bouillons, microscopy bacterium number；
Obtained bloom blue algae extract 5ml in 1.3 dropwise addition above-described embodiments, cultivates 18h at 37 DEG C, microscopy bacterium number,
Obtain result as shown in table 5.
Bacteriostasis rate=(starting bacterium number-starting bacterium number)/starting bacterium number
The bacteriostasis rate test result table of table 5
| Strain | Originate bacterium number (microscopy) | Bacterium number of surviving (microscopy) | Bacteriostasis rate (%) |
| Escherichia coli | 3103 | 270 | 91.3 |
| Pseudomonas aeruginosa | 5000 | 190 | 96.2 |
| Staphylococcus aureus | 2882 | 320 | 88.9 |
As seen from Table 5, the bloom blue algae extract obtained in above-described embodiment has preferable sterilization
Property, wherein Escherichia coli are up to 91.3%, and Pseudomonas aeruginosa is up to 96.2%, and staphylococcus aureus is up to 88.9%.
2. feed functional assays
Using four groups of male white mouses as experiment sample, every group of small white mouse 12, numbering is 1,2,3,4, and 1 group is used embodiment
Forage feed in 1,2 groups with the forage feed in embodiment 2,3 groups with the forage feed in embodiment 3,4 groups with comparative example 1
In raising feed, four groups of other feeding conditions of mouse are consistent, continuous to feed 60 days.
Then four groups of mouse are infected with animal influenza A virus, wherein, there is mouse to occur within 24 hours in 1 group
Flu-like symptom, 2 mouse occurred mouse after mild attack of influenza symptom, and 7 days in 36 hours and fully recovered.
Do not occur flu-like symptom in 2 groups.
There is 1 mouse to occur fully recovering after flu-like symptom, and 7 days in 36 hours in 3 groups.
There are 4 mouse to occur flu-like symptom in 24 hours in 4 groups, 2 mouse flu-like symptom occurred in 36 hours,
Occur wherein one mouse of flu-like symptom in 24 hours, occur symptoms of pneumonia after 3 days, dead 3 mouse after 7 days.
Pass through above-mentioned experimental verification, it can be seen that with the addition of the functional feed of bloom blue algae extract has good exempt from
Epidemic disease strengthens function.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using the present invention.
A variety of modifications to these embodiments will be apparent for those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and principles disclosed herein and features of novelty phase one
The most wide scope caused.
Claims (6)
1. a kind of functional feed for adding bloom blue algae extract, it is characterised in that in parts by weight, including：
Wherein, the pasture and water powder be TAIHU LAKE grass meal, from Taihu Lake salvage collect pasture and water, then cleaning, drying, drying and dewatering,
It is ground into powder；
The preparation method of the bloom blue algae extract includes：
Sieve takes bloom blue algae, then carries out filtering means dehydration to the bloom blue algae for removing large granular impurity, obtains being dehydrated bloom blue algae,
Then the bloom blue algae of dehydration is dried, bloom blue algae powder is obtained after dries pulverizing；
Bloom blue algae powder is added in phosphate buffer, the mass ratio of bloom blue algae powder and phosphate buffer is 2:25, so
After add complex enzyme, complex enzyme is made up of papain, cellulase, pectase, after complex enzyme is digested, and is gone out
It is living, obtain enzymolysis liquid；
Enzymolysis liquid is put into high-shear homogenizer, homogeneous 3min, the solution after homogeneous stood at 2-4 DEG C, standing is not less than
12h；
Centrifuge 6-8min under 4000-6000rpm rotating speeds, obtain first time supernatant, by first time supernatant add ammonium sulfate or
After ammonium carbonate, standing, retain supernatant, continuously add ammonium sulfate or ammonium carbonate to saturation, under 10000-12000rpm rotating speeds from
Heart 10min, after separation, obtains second of supernatant and precipitation；
Precipitation is dissolved with enough distilled water, using ultrafiltration desalination, milipore filter dialysis obtains third time supernatant and precipitation；
After second of supernatant and the mixing centrifugation of third time supernatant, obtain and retain liquid, reservation liquid regulation pH to neutrality, then
Freeze-drying, obtains bloom blue algae extract.
2. a kind of functional feed for adding bloom blue algae extract according to claim 1, it is characterised in that by wawter bloom
Blue-green algae dries pulverizing is obtained after bloom blue algae powder, then bloom blue algae powder is put into nutrient solution cultivated, and is included in nutrient solution：Lemon
Sour iron, molybdic acid, glucose, potassium sulfate, potassium carbonate, the concentration of nutrient solution is 120mg/L, and the pH of nutrient solution is 7, bloom blue algae powder
Cultivated 5 days in nutrient solution, complete akinete induction；
Bloom blue algae powder is in akinete Induction Process, under sterilized environment, and timing agitation once, after 5 days, will pass through heavy wall
Bloom blue algae after Spore inducing is placed in 30-40 DEG C of baking oven and dried, and obtains the bloom blue algae induced by akinete
Powder, the bloom blue algae powder after akinete induces is used during enzymolysis obtains bloom blue algae extract.
3. a kind of functional feed for adding bloom blue algae extract according to claim 1, it is characterised in that described multiple
The vigor of papain, cellulase, three kinds of enzymes of pectase in synthase is respectively 100000 unit meters.
4. a kind of functional feed for adding bloom blue algae extract according to claim 1, it is characterised in that use institute
PH when stating complex enzyme zymohydrolysis is 5-9, and temperature during enzymolysis is 45-65 DEG C.
5. a kind of functional feed for adding bloom blue algae extract according to claim 1, it is characterised in that described auxiliary
Feed ingredient is helped to include：Fish protein active peptides, pumpkin seed powder, China tree, folium artemisiae argyi, cordate houttuynia.
6. a kind of functional feed for adding bloom blue algae extract according to claim 1, it is characterised in that the dimension
Raw element includes：Vitamin A, vitamin B6, vitamin B12, vitamin B2, vitamin D, vitamin E.
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| WO2022110088A1 (en) * | 2020-11-28 | 2022-06-02 | 南京溧水高新创业投资管理有限公司 | Culture medium for screening high-yield gibberellin strain |
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| Publication number | Publication date |
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| CN107047972B (en) | 2020-12-11 |
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