CN101863720A - Microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof - Google Patents
Microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof Download PDFInfo
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- CN101863720A CN101863720A CN201010204561A CN201010204561A CN101863720A CN 101863720 A CN101863720 A CN 101863720A CN 201010204561 A CN201010204561 A CN 201010204561A CN 201010204561 A CN201010204561 A CN 201010204561A CN 101863720 A CN101863720 A CN 101863720A
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Abstract
The invention discloses a microbial fertilizer for holding water and fixing nitrogen of soil and a preparation method thereof. The microbial fertilizer comprises an organism-based high polymer and an organic matter, wherein the organism-based high polymer is prepared from the following raw materials: 7 to 10 of acrylic acid, 1 to 4 of potassium hydroxide, 2 to 5 of starch, 1.5 to 8 of water, 29 to 97.5 of Bacillus mucilaginosus liquid and 0.17 to 0.2 of ammonium persulfate; and the organic matter comprises 10 to 20 of grass carbon, 20 to 40 of brown coal, 20 to 50 of bentonite and 30 to 50 of humus earth. The microbial fertilizer integrates phosphorus dissolving, potassium dissolving, nitrogen fixing and water holding, can reduce fertilizer consumption by 50 percent when used in the soil, keep the soil moist, loose and soft, improve the air permeability and organic matter content of the soil, increase the soil aggregate year by year to improve the soil, improve the comprehensive productivity of the soil and increase the yield per mu by 20 to 30 percent, and also can improve the content of starch, proteins and vitamins in fruits and vegetables when used in the soil for growing the fruits and vegetables.
Description
Technical field
The present invention relates to a kind of microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof, can be widely used in agricultural, gardens, soil improvement, preserve moisture and fertility, the engineering of conceding the land to forestry and grass, defend and control sand.
Background technology
At present, the fertilizer that farm crop are used mostly is chemical fertilizer such as inorganic fertilizer, compoiste fertilizer greatly, owing to use nitrogen, phosphorus, potassium three elements chemical fertilizer all the year round, has made the soil microorganisms eubiosis cause havoc.High-density insolubles precipitation is solidified, soil compaction, and the preserve moisture and fertility ability drop, soil activation and organic content reduce, and the farm crop desired nutritional is supplied with imbalance, and many effective nutritive substances are suppressed.The chemical fertilizer mu consumption of China constantly increases, and the mu effect of increasing production is not obvious, and the chemical fertilizer waste is serious, the annual utilization rate of nitrogen fertilizer that uses of China only is 30-35%, and phosphate fertilizer only is 10-20%, and potash fertilizer is 35-50%, compare with external chemical fertilizer average utilization 60%, have a long way to go.For this reason, people have used the fixed nitrogen technology, wish nitrogen fixing and slowly release in soil.But existing fixed nitrogen technology mostly is the vinelandii biological nitrogen fixation, and its nitrogen fixation effect is not obvious, does not satisfy the plant-growth needs, also wants chemical nitrogen to replenish.Slowly-releasing nitrogen technology is a coated with resin in the existing chemical fertilizer, and the coating dissolving discharges then in soil, the initial release time that it has just delayed nitrogen, do not prolong time of releasing of nitrogen, and the loss of nitrogen is the same with common chemical fertilizer.At present the microbial fertilizer that uses mostly is phosphorus bacteria and potassium bacterium, and bacterial strain is by liquid submerged fermentation and amplify step by step and obtain bacterium liquid, can P and K decomposing, but can not economize on water by fixed nitrogen, effect is single.
At present, the deterioration in China along with physical environment, climate warming, arid, phreatic exhausted day by day, plant husbandry, greening, sand control all are faced with acid test, especially need a kind of resisting drought saving water technology, particularly the western arid area of China.The resisting drought saving water technology of China mostly is the engineering type at present, as: building anti seepage channel, sprinkling irrigation, drip irrigation etc., is exactly agrotype in addition, as: film coating technique, water cellar water storage etc.But all there is certain drawback in above various water-saving technology on using, wherein engineering type water-saving technology quantities is big, and investment is big, as does not have country or pool that to build be to be difficult to realize the waste that exists evaporation simultaneously and leak.Though the agrotype water-saving technology is practical, on the sand soil that leaks, still can causes the waste of water, and have pollution problem.At present up-to-date water-saving technology is to use the high molecular polymer water-holding agent, and it utilizes solution method or suspension method to obtain by polyreaction, and the resisting drought saving water effect is obvious, but can not P and K decomposing and fixed nitrogen, and effect is single, the cost height.
Summary of the invention
Purpose of the present invention solves the above-mentioned problems in the prior art exactly, provides a kind of collected explanations or commentaries phosphorus, potassium decomposing, fixed nitrogen in one, saving water, resisting drought outstanding effect microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof.
For finishing purpose of the present invention, technical solution of the present invention is: a kind of microbial fertilizer for holding water and fixing nitrogen of soil, and it comprises bio-based high molecular polymer and organic matter; By weight calculating, the bio-based high molecular polymer is made by following raw material: vinylformic acid 7-10, potassium hydroxide 1-4, starch 2-5, water 1.5-8, Bacillus mucilaginosus bacterium liquid 29-97.5 and ammonium persulphate 0.17-0.2; Organic matter comprises peat composed of rotten mosses 10-20, brown coal 20-40, wilkinite 20-50 and vegetable mould 30-50.
The making method of above-mentioned described microbial fertilizer for holding water and fixing nitrogen of soil, it may further comprise the steps:
(1) Bacillus mucilaginosus is made bacterium liquid by fermentation culture; The used substratum that ferments is mixed with by weight by following material: Na
2HPO
41.1-2.3, MgSO
41.3-1.7, potassium felspar sand 1-3, agar 17-20, K
2HPO
41.1-1.9, NaCl 2-5, glucose 13-21, CaCO
33-7 and water 23-70.
(2) vinylformic acid, potassium hydroxide, starch, the water with above-mentioned described amount places the stirring in-tank mixing to stir, fully after the neutralization, the ammonium persulphate that when temperature reaches 50-55 ℃, adds described amount, insert in the biochemical polymerization reactor, the Bacillus mucilaginosus bacterium liquid that adds described amount then mixes, by the register controlled temperature 42-47 ℃ of following low-temperature atomizing polymerization, obtain containing the bio-based high molecular polymer of glue peptone sample bacillus, again by the peat composed of rotten mosses, brown coal, wilkinite and the vegetable mould that adds described amount after the liquid nitrogen cryogenics drying stir powder or particle.
The present invention mixes the Bacillus mucilaginosus microbial inoculum with the simple of high molecular polymer, but crosslinked together by polyreaction bacterium liquid that contains Bacillus mucilaginosus and neutralization solution, has guaranteed the activity of microorganism strains.
Product of the present invention can adsorb nitrogen and moisture, makes nitrogen and the moisture can very fast volatilization, but slowly discharges according to the needs of soil, prolongs the time of releasing of nitrogen, does not take off fertilizer, and nitrogen and efficiency of water application height have reduced waste, have saved fertilizer and water resources.The all right phosphorus decomposing of product of the present invention, potassium decomposing, and be adsorbed slow release, reduce chemical phosphorus, potassium fixing in soil, thereby reduce fertilizer amount, reduce pollution.Can decompose simultaneously several kinds of mineral elements confession plant utilization in the soil, comprehensive nutrition, fertilizer efficiency is lasting.Collected explanations or commentaries phosphorus of the present invention, potassium decomposing, fixed nitrogen and water conservation are in one, in soil, use product of the present invention, can reduce by 50% fertilizer amount, simultaneously can keep soil moistening for a long time, soft, improve the ventilation property and the organic content of soil, increase soil aggregate year by year, play the effect of improving the soil, and the integrated production capacity of soil is improved, can make per mu yield improve 20-30%.It is used for planting the soil of gourd, fruit and vegetable, also can improve the content of starch in the gourd, fruit and vegetable, protein, VITAMIN, increases the melon and fruit sugariness.
Embodiment
The present invention will be further described below in conjunction with specific embodiment:
Embodiment 1, and present embodiment comprises bio-based high molecular polymer and organic matter.By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 7, potassium hydroxide 4, starch 2, water 1.5, Bacillus mucilaginosus bacterium liquid 97.5 and ammonium persulphate 0.2; Organic matter comprises the peat composed of rotten mosses 20, brown coal 40, wilkinite 20 and vegetable mould 30.
Its making method is that it may further comprise the steps:
(1) Bacillus mucilaginosus is made bacterium liquid by fermentation culture.Adopt the heat-shock method to handle Bacillus mucilaginosus, improve seed uniformity, shorten fermentation period to 27 hour, at first press Na
2HPO
41.1, MgSO
41.7, potassium felspar sand 1, agar 20, K
2HPO
41.9, NaCl 5, glucose 13, CaCO
37, the part by weight of water 23 places preparation substratum in the fermentor tank, heats by steam then, when the jar temperature reaches 33-37 ℃, keeps tank pressure 07.0-1.4kg/cm2, inoculation glue peptone sample bacterial strain of bacillus; Pay close attention to temperature and tank pressure and keep fermentation 20-24 hour, obtain the bacterium liquid that bacteria containing amount reaches 700,000,000/milliliter.
(2) vinylformic acid, potassium hydroxide, starch, the water with above-mentioned described amount places the stirring in-tank mixing to stir, fully after the neutralization, the ammonium persulphate that when temperature reaches 50-55 ℃, adds described amount, enter biochemical polymerization reactor, the Bacillus mucilaginosus bacterium liquid that adds described amount then mixes, 42-47 ℃ of following low-temperature atomizing polymerization, obtain containing the polyacrylic acid potassium of glue peptone sample bacillus, again by the peat composed of rotten mosses, brown coal, wilkinite and the vegetable mould that adds described amount after the liquid nitrogen cryogenics drying stir powder or particle.
Embodiment 2, and present embodiment comprises bio-based high molecular polymer and organic matter.By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 8, potassium hydroxide 2, starch 3, water 5, Bacillus mucilaginosus bacterium liquid 55 and ammonium persulphate 0.18; Organic matter comprises the peat composed of rotten mosses 15, brown coal 30, wilkinite 30 and vegetable mould 40.
Its making method is with embodiment 1.Just Bacillus mucilaginosus being made the used substratum of bacterium liquid by fermentation culture is mixed with by following material: Na
2HPO
41.7, MgSO
41.5, potassium felspar sand 2, agar 18, K
2HPO
41.6, NaCl 3, glucose 15, CaCO
35, water 50.
Embodiment 3, and present embodiment comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 10, potassium hydroxide 2.5, starch 4, water 3, Bacillus mucilaginosus bacterium liquid 29 and ammonium persulphate 0.17; Organic matter comprises the peat composed of rotten mosses 10, brown coal 20, wilkinite 50 and vegetable mould 50.
Its making method is with embodiment 1.Just Bacillus mucilaginosus being made the used substratum of bacterium liquid by fermentation culture is mixed with by following material: Na
2HPO
42, MgSO
41.3, potassium felspar sand 3, agar 17, K
2HPO
41.1, NaCl 2, glucose 21, CaCO
33, water 70.
Embodiment 4, and present embodiment comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 7.5, potassium hydroxide 3, starch 5, water 8, Bacillus mucilaginosus bacterium liquid 40 and ammonium persulphate 0.19; Organic matter comprises the peat composed of rotten mosses 17.5, brown coal 35, wilkinite 40 and vegetable mould 35.
Its making method is with embodiment 1.Just Bacillus mucilaginosus being made the used substratum of bacterium liquid by fermentation culture is mixed with by following material: Na
2HPO
42.3, MgSO
41.4, potassium felspar sand 2.5, agar 19, K
2HPO
41.8, NaCl4, glucose 17, CaCO
34, water 40.
Embodiment 5, and present embodiment comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 8.5, potassium hydroxide 1, starch 3.5, water 6.5, Bacillus mucilaginosus bacterium liquid 75 and ammonium persulphate 0.185; Organic matter comprises the peat composed of rotten mosses 12.5, brown coal 25, wilkinite 35 and vegetable mould 45.
Its making method is with embodiment 1.Just Bacillus mucilaginosus being made the used substratum of bacterium liquid by fermentation culture is mixed with by following material: Na
2HPO
41.5, MgSO
41.6, potassium felspar sand 1.5, agar 18.5, K
2HPO
41.4, NaCl3.5, glucose 19, CaCO
36, water 60.
Claims (9)
1. microbial fertilizer for holding water and fixing nitrogen of soil, it is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating, the bio-based high molecular polymer is made by following raw material: vinylformic acid 7-10, potassium hydroxide 1-4, starch 2-5, water 1.5-8, Bacillus mucilaginosus bacterium liquid 29-97.5 and ammonium persulphate 0.17-0.2; Organic matter comprises peat composed of rotten mosses 10-20, brown coal 20-40, wilkinite 20-50 and vegetable mould 30-50.
2. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 7, potassium hydroxide 4, starch 2, water 1.5, Bacillus mucilaginosus bacterium liquid 97.5 and ammonium persulphate 0.2; Organic matter comprises the peat composed of rotten mosses 20, brown coal 40, wilkinite 20 and vegetable mould 30.
3. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter.By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 8, potassium hydroxide 2, starch 3, water 5, Bacillus mucilaginosus bacterium liquid 55 and ammonium persulphate 0.18; Organic matter comprises the peat composed of rotten mosses 15, brown coal 30, wilkinite 30 and vegetable mould 40.
4. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 10, potassium hydroxide 2.5, starch 4, water 3, Bacillus mucilaginosus bacterium liquid 29 and ammonium persulphate 0.17; Organic matter comprises the peat composed of rotten mosses 10, brown coal 20, wilkinite 50 and vegetable mould 50.
5. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 7.5, potassium hydroxide 3, starch 5, water 8, Bacillus mucilaginosus bacterium liquid 40 and ammonium persulphate 0.19; Organic matter comprises the peat composed of rotten mosses 17.5, brown coal 35, wilkinite 40 and vegetable mould 35.
6. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is made by following raw material: vinylformic acid 8.5, potassium hydroxide 1, starch 3.5, water 6.5, Bacillus mucilaginosus bacterium liquid 75 and ammonium persulphate 0.185; Organic matter comprises the peat composed of rotten mosses 12.5, brown coal 25, wilkinite 35 and vegetable mould 45.
7. the making method of each the described microbial fertilizer for holding water and fixing nitrogen of soil among the claim 1-6, it comprises that step (1) makes bacterium liquid to Bacillus mucilaginosus by fermentation culture; It is characterized in that: it also comprises the vinylformic acid of step (2) with described amount, potassium hydroxide, starch, water places and stirs the in-tank mixing stirring, fully after the neutralization, the ammonium persulphate that when temperature reaches 50-55 ℃, adds described amount, insert in the biochemical polymerization reactor, the Bacillus mucilaginosus bacterium liquid that adds described amount then mixes, by the register controlled temperature 42-47 ℃ of following low-temperature atomizing polymerization, obtain containing the bio-based high molecular polymer of glue peptone sample bacillus, again by adding the peat composed of rotten mosses of described amount after the liquid nitrogen cryogenics drying, brown coal, wilkinite and vegetable mould stir powder or particle.
8. the making method of microbial fertilizer for holding water and fixing nitrogen of soil according to claim 7, it is characterized in that: the method for in the step (1) Bacillus mucilaginosus being made bacterium liquid by fermentation culture is: at first press Na
2HPO
41.1-2.3, MgSO
41.3-1.7, potassium felspar sand 1-3, agar 17-20, K
2HPO
41.1-1.9, NaCl 2-5, glucose 13-21, CaCO
3The part by weight of 3-7 and water 23-70 places preparation substratum in the fermentor tank, heats by steam then, when the jar temperature reaches 33-37 ℃, keeps tank pressure 07.0-1.4kg/cm2, inoculation glue peptone sample bacterial strain of bacillus; Pay close attention to temperature and tank pressure and keep fermentation 20-24 hour, obtain bacterium liquid.
9. the making method of microbial fertilizer for holding water and fixing nitrogen of soil according to claim 8, it is characterized in that: the bacteria containing amount of described bacterium liquid reaches 700,000,000/milliliter.
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|---|---|---|---|
| CN201010204561A CN101863720B (en) | 2010-06-22 | 2010-06-22 | Microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof |
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| CN101863720B CN101863720B (en) | 2012-10-24 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515890A (en) * | 2012-01-05 | 2012-06-27 | 中国农业大学 | Composition for promoting nodulation and nitrogen fixation of leguminous plant and preparation method thereof |
| CN103787781A (en) * | 2014-02-27 | 2014-05-14 | 王东 | High-efficiency anti-seepage fertilizer and preparation method thereof |
| CN105130639A (en) * | 2015-08-25 | 2015-12-09 | 全椒林农生态农业科技有限公司 | Apocarya planting soil improver and soil improving method |
| CN105884427A (en) * | 2016-04-21 | 2016-08-24 | 张楚沁 | Improved acidified soil water-retention compound fertilizer and preparation method thereof |
| CN109956821A (en) * | 2019-03-27 | 2019-07-02 | 广州市永添生物科技有限公司 | It is a kind of for enhancing the preparation method of the enhancement particles of microorganism fertilizer |
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| JPH01285119A (en) * | 1988-05-11 | 1989-11-16 | Sanyo Chem Ind Ltd | Water-retaining agent for soil and water-retaining method |
| CN1475467A (en) * | 2002-08-14 | 2004-02-18 | 吴晓丽 | Bamboo forest biological fertilizer |
| CN101215539A (en) * | 2008-01-07 | 2008-07-09 | 周文彩 | Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof |
-
2010
- 2010-06-22 CN CN201010204561A patent/CN101863720B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01285119A (en) * | 1988-05-11 | 1989-11-16 | Sanyo Chem Ind Ltd | Water-retaining agent for soil and water-retaining method |
| CN1475467A (en) * | 2002-08-14 | 2004-02-18 | 吴晓丽 | Bamboo forest biological fertilizer |
| CN101215539A (en) * | 2008-01-07 | 2008-07-09 | 周文彩 | Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515890A (en) * | 2012-01-05 | 2012-06-27 | 中国农业大学 | Composition for promoting nodulation and nitrogen fixation of leguminous plant and preparation method thereof |
| CN103787781A (en) * | 2014-02-27 | 2014-05-14 | 王东 | High-efficiency anti-seepage fertilizer and preparation method thereof |
| CN105130639A (en) * | 2015-08-25 | 2015-12-09 | 全椒林农生态农业科技有限公司 | Apocarya planting soil improver and soil improving method |
| CN105884427A (en) * | 2016-04-21 | 2016-08-24 | 张楚沁 | Improved acidified soil water-retention compound fertilizer and preparation method thereof |
| CN109956821A (en) * | 2019-03-27 | 2019-07-02 | 广州市永添生物科技有限公司 | It is a kind of for enhancing the preparation method of the enhancement particles of microorganism fertilizer |
| CN109956821B (en) * | 2019-03-27 | 2021-06-04 | 沈阳丰恒泰农业科技有限公司 | Preparation method of synergistic particles for enhancing microbial fertilizer |
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| Publication number | Publication date |
|---|---|
| CN101863720B (en) | 2012-10-24 |
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