CL2012002007A1 - Método para eliminar una región de adn en una planta, que comprende proporcionar una planta que comprende dicha región de adn en su genoma, la cual expresa una nucleasa de ‘dedo de zinc’ diseñada para disociar el adn genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc. - Google Patents
Método para eliminar una región de adn en una planta, que comprende proporcionar una planta que comprende dicha región de adn en su genoma, la cual expresa una nucleasa de ‘dedo de zinc’ diseñada para disociar el adn genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc.Info
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- CL2012002007A1 CL2012002007A1 CL2012002007A CL2012002007A CL2012002007A1 CL 2012002007 A1 CL2012002007 A1 CL 2012002007A1 CL 2012002007 A CL2012002007 A CL 2012002007A CL 2012002007 A CL2012002007 A CL 2012002007A CL 2012002007 A1 CL2012002007 A1 CL 2012002007A1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8265—Transgene containment, e.g. gene dispersal
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Botany (AREA)
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- Environmental Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Cultivation Of Plants (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método para eliminar una región de ADN en una planta, que comprende proporcionar una planta que comprende dicha región de ADN en su genoma, la cual expresa una nucleasa de ""Dedo de Zinc"" diseñada para disociar el ADN genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29762810P | 2010-01-22 | 2010-01-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
CL2012002007A1 true CL2012002007A1 (es) | 2012-10-19 |
Family
ID=44307617
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CL2012002007A CL2012002007A1 (es) | 2010-01-22 | 2012-07-19 | Método para eliminar una región de adn en una planta, que comprende proporcionar una planta que comprende dicha región de adn en su genoma, la cual expresa una nucleasa de ‘dedo de zinc’ diseñada para disociar el adn genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc. |
Country Status (19)
Country | Link |
---|---|
US (3) | US9695432B2 (es) |
EP (1) | EP2525649B1 (es) |
JP (2) | JP2013517774A (es) |
KR (1) | KR101951489B1 (es) |
CN (1) | CN102843904B (es) |
AR (1) | AR079968A1 (es) |
AU (3) | AU2011207387B2 (es) |
BR (1) | BR112012017896A2 (es) |
CA (1) | CA2787674C (es) |
CL (1) | CL2012002007A1 (es) |
CO (1) | CO6592070A2 (es) |
EA (1) | EA031356B1 (es) |
GE (1) | GEP201606544B (es) |
IL (2) | IL220870A (es) |
MX (1) | MX2012008485A (es) |
NZ (1) | NZ600546A (es) |
UA (1) | UA113493C2 (es) |
WO (1) | WO2011091311A2 (es) |
ZA (1) | ZA201204519B (es) |
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EP2568048A1 (en) * | 2007-06-29 | 2013-03-13 | Pioneer Hi-Bred International, Inc. | Methods for altering the genome of a monocot plant cell |
DK2205749T3 (en) | 2007-09-27 | 2016-08-22 | Dow Agrosciences Llc | MODIFIED PROTEINS zinc finger, which target the 5-enolpyruvylshikimate-3-phosphate synthase genes |
MX341747B (es) * | 2008-02-29 | 2016-08-31 | Monsanto Technology Llc | Evento de maiz mon87460 y composiciones y metodos para detectarlo. |
EP2298883B1 (en) * | 2008-05-28 | 2016-07-27 | Kyoto University | Novel selection marker gene and use thereof |
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