AR079968A1 - Escision de transgenes en plantas geneticamente modificadas - Google Patents

Escision de transgenes en plantas geneticamente modificadas

Info

Publication number
AR079968A1
AR079968A1 ARP110100222A ARP110100222A AR079968A1 AR 079968 A1 AR079968 A1 AR 079968A1 AR P110100222 A ARP110100222 A AR P110100222A AR P110100222 A ARP110100222 A AR P110100222A AR 079968 A1 AR079968 A1 AR 079968A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
plant
plants
zfns
excision
Prior art date
Application number
ARP110100222A
Other languages
English (en)
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44307617&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR079968(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR079968A1 publication Critical patent/AR079968A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265Transgene containment, e.g. gene dispersal
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para suprimir una region de ADN en una planta. En algunas formas de realizacion, el método comprende la transformacion de una planta con una molécula de ácido nucleico, en donde la molécula de ácido nucleico codifica una o varias nucleasas de dedos de zinc (ZFN) operativamente ligadas a uno o varios promotores específicos de tejidos, por ejemplo, un promotor específico de polen. Los métodos incluyen la escision de genes nativos en una planta. Conforme a ello, en algunas formas de realizacion, ZFN se manipulan para que reconozcan secuencias que flanquean genes de plantas nativos. En otras formas de realizacion, las ZFN se expresan bajo el control de promotores específicos del estadio de desarrollo, es decir, por ejemplo, secuencias de ácidos nucleicos se eliminan específicamente en plantas durante estadios de desarrollo relativamente tardíos. Se incluyen las moléculas de ácido nucleico de utilidad para llevar a cabo los métodos revelados y las plantas producidas por los métodos.
ARP110100222A 2010-01-22 2011-01-24 Escision de transgenes en plantas geneticamente modificadas AR079968A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29762810P 2010-01-22 2010-01-22

Publications (1)

Publication Number Publication Date
AR079968A1 true AR079968A1 (es) 2012-02-29

Family

ID=44307617

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110100222A AR079968A1 (es) 2010-01-22 2011-01-24 Escision de transgenes en plantas geneticamente modificadas

Country Status (19)

Country Link
US (3) US9695432B2 (es)
EP (1) EP2525649B1 (es)
JP (2) JP2013517774A (es)
KR (1) KR101951489B1 (es)
CN (1) CN102843904B (es)
AR (1) AR079968A1 (es)
AU (3) AU2011207387B2 (es)
BR (1) BR112012017896A2 (es)
CA (1) CA2787674C (es)
CL (1) CL2012002007A1 (es)
CO (1) CO6592070A2 (es)
EA (1) EA031356B1 (es)
GE (1) GEP201606544B (es)
IL (2) IL220870A (es)
MX (1) MX2012008485A (es)
NZ (1) NZ600546A (es)
UA (1) UA113493C2 (es)
WO (1) WO2011091311A2 (es)
ZA (1) ZA201204519B (es)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GEP20176628B (en) * 2010-01-22 2017-02-27 Sangamo Biosciences Inc Targeted genomic alteration
US10030245B2 (en) 2011-03-23 2018-07-24 E I Du Pont De Nemours And Company Methods for producing a complex transgenic trait locus
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
BR112014003919A2 (pt) * 2011-08-22 2017-03-14 Bayer Cropscience Ag métodos e meios para modificar um genoma de planta
BR102013001773A2 (pt) 2012-01-23 2015-09-08 Dow Agrosciences Llc evento de algodão pdab4468.19.10.3 tolerante a herbicidas
WO2013163394A1 (en) 2012-04-25 2013-10-31 Regeneron Pharmaceuticals, Inc. Nuclease-mediated targeting with large targeting vectors
UA119135C2 (uk) * 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
RU2678001C2 (ru) * 2012-12-13 2019-01-22 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Способы обнаружения днк для сайт-специфической нуклеазной активности
CN111500630A (zh) 2013-04-16 2020-08-07 瑞泽恩制药公司 大鼠基因组的靶向修饰
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
RU2668819C2 (ru) 2013-09-04 2018-10-02 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Быстрый направленный анализ сельскохозяйственных культур для определения донорной вставки
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
KR102248730B1 (ko) 2013-11-04 2021-05-07 코르테바 애그리사이언스 엘엘씨 유전자 표적화를 위한 범용 공여자 시스템
US9546384B2 (en) 2013-12-11 2017-01-17 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a mouse genome
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
CN113215196A (zh) 2014-06-06 2021-08-06 瑞泽恩制药公司 用于修饰所靶向基因座的方法和组合物
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
CN108064129A (zh) 2014-09-12 2018-05-22 纳幕尔杜邦公司 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法
PT3221457T (pt) 2014-11-21 2019-06-27 Regeneron Pharma Métodos e composições para modificação genética visada através da utilização de arn guia emparelhados
CN108513575A (zh) 2015-10-23 2018-09-07 哈佛大学的校长及成员们 核碱基编辑器及其用途
US10781455B2 (en) 2016-06-20 2020-09-22 Dow Agrosciences Llc Methodologies and compositions for creating targeted recombination and breaking linkage between traits
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
EP3541168A4 (en) * 2016-11-21 2020-06-17 Dow AgroSciences LLC LOCATION-SPECIFIC INTEGRATION OF A TRANSGEN WITH INTRAGENOMIC RECOMBINATION VIA A NON-HOMOLOGICAL FINAL REPAIR SIGNAL WAY
EP3555285A4 (en) * 2016-12-14 2020-07-08 Dow AgroSciences LLC RECONSTRUCTION OF JOB-SPECIFIC NUCLEASE BINDING POINTS
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
SG11201908658TA (en) 2017-03-23 2019-10-30 Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
DE112020001342T5 (de) 2019-03-19 2022-01-13 President and Fellows of Harvard College Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen
US20220298519A1 (en) * 2019-06-19 2022-09-22 Pioneer Hi-Bred International, Inc. Pod shatter tolerance in brassica plants
EP4146804A1 (en) 2020-05-08 2023-03-15 The Broad Institute Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
US11242534B1 (en) 2020-07-31 2022-02-08 Inari Agriculture Technology, Inc. INHT31 transgenic soybean
BR112023001798A2 (pt) * 2020-07-31 2023-02-23 Inari Agriculture Tech Inc Célula de planta de milho transgênico, parte de planta de milho transgênico, planta de milho transgênico, método para obter uma população a granel de sementes endogâmicas, método de obtenção de semente de milho híbrido, molécula de dna, produto de planta de milho transgênico processado, amostra biológica, molécula de ácido nucleico adaptada para detecção de dna genômico, método de detecção de uma célula de planta de milho, método de excisão do lócus transgênico inir12 do genoma da célula de planta de milho, método de modificação de uma célula de planta de milho transgênico, método de produção de células de plantas de milho transgênico compreendendo um lócus transgênico inir12, calo de planta de milho transgênico, semente de planta de milho transgênico, método de uso da célula de planta de milho, método de coleta de dados de análise de ácidos nucleicos, método de reprodução de plantas
US20240011043A1 (en) 2020-07-31 2024-01-11 Inari Agriculture Technology, Inc. Generation of plants with improved transgenic loci by genome editing
US11753677B2 (en) 2021-11-10 2023-09-12 Encodia, Inc. Methods for barcoding macromolecules in individual cells

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
EP0222493A1 (en) 1985-10-04 1987-05-20 Lubrizol Genetics Inc. TR-based sub-TI plasmids
US5750871A (en) 1986-05-29 1998-05-12 Calgene, Inc. Transformation and foreign gene expression in Brassica species
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
DE3784860D1 (de) 1986-12-05 1993-04-22 Ciba Geigy Ag Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
US5359142A (en) 1987-01-13 1994-10-25 Monsanto Company Method for enhanced expression of a protein
US5322938A (en) 1987-01-13 1994-06-21 Monsanto Company DNA sequence for enhancing the efficiency of transcription
US5244802A (en) 1987-11-18 1993-09-14 Phytogen Regeneration of cotton
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6051753A (en) 1989-09-07 2000-04-18 Calgene, Inc. Figwort mosaic virus promoter and uses
EP0426641B1 (en) 1989-10-31 2000-09-13 Monsanto Company Promoter for transgenic plants
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
HU220773B1 (hu) 1990-01-22 2002-05-28 Dekalb Genetics Corporation Eljárás termő transzgenikus kukoricanövények előállítására
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US7060876B2 (en) 1992-07-07 2006-06-13 Japan Tobacco Inc. Method for transforming monocotyledons
WO1994000977A1 (en) 1992-07-07 1994-01-20 Japan Tobacco Inc. Method of transforming monocotyledon
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
EP0672752B1 (en) 1993-09-03 2004-05-26 Japan Tobacco Inc. Method of transforming monocotyledon by using scutellum of immature embryo
US5635055A (en) 1994-07-19 1997-06-03 Exxon Research & Engineering Company Membrane process for increasing conversion of catalytic cracking or thermal cracking units (law011)
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5723765A (en) 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5846797A (en) 1995-10-04 1998-12-08 Calgene, Inc. Cotton transformation
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5850019A (en) 1996-08-06 1998-12-15 University Of Kentucky Research Foundation Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants
WO1998010080A1 (en) 1996-09-05 1998-03-12 Unilever N.V. Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein
HUP0000922A3 (en) 1997-01-20 2002-03-28 Plant Genetic Systems Nv Pathogen-induced plant promoters
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US5922564A (en) 1997-02-24 1999-07-13 Performance Plants, Inc. Phosphate-deficiency inducible promoter
CN1301300A (zh) 1998-02-20 2001-06-27 曾尼卡有限公司 花粉特异性启动子
CA2315549A1 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
AU762993C (en) 1998-02-26 2004-06-10 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
JP2000083680A (ja) 1998-07-16 2000-03-28 Nippon Paper Industries Co Ltd 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ―
EP1141346A2 (en) 1999-01-14 2001-10-10 Monsanto Co. Soybean transformation method
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6677503B1 (en) 1999-06-23 2004-01-13 Pioneer Hi-Bred International, Inc. Sunflower anti-pathogene proteins and genes and their uses
JP2004512007A (ja) * 2000-02-28 2004-04-22 エール ユニヴァーシティ トランス遺伝子の伝達を削減または排除する方法および組成物
US6388170B1 (en) 2000-04-07 2002-05-14 University Of Kentucky Research Foundation Bidirectional promoters and methods related thereto
CA2411649C (en) * 2001-04-17 2009-04-07 Icon Genetics, Inc. Ires enabled gene trapping in plants
BRPI0307383B1 (pt) * 2002-01-23 2019-12-31 The Univ Of Utah Research Foundation método de recombinação genética direcionada em célula de planta hospedeira
CA2445291A1 (en) 2002-02-07 2003-08-14 Hybrigene, Inc Prevention of transgene escape in genetically modified perennials
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
PL1689870T3 (pl) 2003-11-18 2009-06-30 Bayer Cropscience Nv Ulepszona docelowa insercja DNA w roślinach
WO2006105946A2 (en) 2005-04-04 2006-10-12 Bayer Bioscience N.V. Methods and means for removal of a selected dna sequence
SG10201508995QA (en) * 2005-07-26 2015-11-27 Sangamo Biosciences Inc Targeted integration and expression of exogenous nucleic acid sequences
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
WO2008145757A1 (en) * 2007-05-31 2008-12-04 Vrije Universiteit Brussel Targeted genome modifications in plants
EP2568048A1 (en) * 2007-06-29 2013-03-13 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
DK2205749T3 (en) 2007-09-27 2016-08-22 Dow Agrosciences Llc MODIFIED PROTEINS zinc finger, which target the 5-enolpyruvylshikimate-3-phosphate synthase genes
MX341747B (es) * 2008-02-29 2016-08-31 Monsanto Technology Llc Evento de maiz mon87460 y composiciones y metodos para detectarlo.
EP2298883B1 (en) * 2008-05-28 2016-07-27 Kyoto University Novel selection marker gene and use thereof

Also Published As

Publication number Publication date
UA113493C2 (xx) 2017-02-10
NZ600546A (en) 2014-08-29
CN102843904A (zh) 2012-12-26
JP2013517774A (ja) 2013-05-20
CA2787674C (en) 2022-09-06
BR112012017896A2 (pt) 2015-09-01
AU2011207387B2 (en) 2015-02-26
AU2011207387A1 (en) 2012-07-05
US20110191877A1 (en) 2011-08-04
IL248459B (en) 2018-06-28
AU2015202859A1 (en) 2015-06-18
US20190112614A1 (en) 2019-04-18
EP2525649A2 (en) 2012-11-28
CA2787674A1 (en) 2011-07-28
WO2011091311A3 (en) 2011-11-17
CO6592070A2 (es) 2013-01-02
GEP201606544B (en) 2016-09-26
IL220870A0 (en) 2012-09-24
EP2525649B1 (en) 2020-01-08
KR101951489B1 (ko) 2019-04-22
US9695432B2 (en) 2017-07-04
JP2016144458A (ja) 2016-08-12
EA201290678A1 (ru) 2012-12-28
MX2012008485A (es) 2012-08-17
ZA201204519B (en) 2013-09-25
US20160257967A1 (en) 2016-09-08
CN102843904B (zh) 2015-01-21
JP6420270B2 (ja) 2018-11-07
EA031356B1 (ru) 2018-12-28
AU2015202859B2 (en) 2017-10-19
CL2012002007A1 (es) 2012-10-19
WO2011091311A2 (en) 2011-07-28
KR20120116494A (ko) 2012-10-22
EP2525649A4 (en) 2013-07-31
IL220870A (en) 2016-11-30
AU2017265039A1 (en) 2017-12-07

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