BR0212886A - Célula bacteriana, método para a produção de um polipeptìdeo heterólogo, método para a prevenção de clivagem, métodos para a clivagem e métodos para a produção de um polipeptìdeo clivado - Google Patents
Célula bacteriana, método para a produção de um polipeptìdeo heterólogo, método para a prevenção de clivagem, métodos para a clivagem e métodos para a produção de um polipeptìdeo clivadoInfo
- Publication number
- BR0212886A BR0212886A BR0212886-1A BR0212886A BR0212886A BR 0212886 A BR0212886 A BR 0212886A BR 0212886 A BR0212886 A BR 0212886A BR 0212886 A BR0212886 A BR 0212886A
- Authority
- BR
- Brazil
- Prior art keywords
- polypeptide
- methods
- producing
- cleavage
- bacterial cell
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 9
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 9
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 8
- 230000001580 bacterial effect Effects 0.000 title abstract 4
- 238000003776 cleavage reaction Methods 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 230000007017 scission Effects 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 102000004400 Aminopeptidases Human genes 0.000 abstract 3
- 108090000915 Aminopeptidases Proteins 0.000 abstract 3
- 230000002759 chromosomal effect Effects 0.000 abstract 2
- 230000002950 deficient Effects 0.000 abstract 2
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
Abstract
"CéLULA BACTERIANA, MéTODO PARA A PRODUçãO DE UM POLIPEPTìDEO HETERóLOGO, MéTODO PARA A PREVENçãO DE CLIVAGEM, MéTODOS PARA A CLIVAGEM E MéTODOS PARA A PRODUçãO DE UM POLIPEPTìDEO CLIVADO". é descrita uma célula bacteriana gram-negativa que é deficiente do um gene cromossómico presente em uma célula de tipo selvagem, em que o gene compartilha pelo menos 80% de identidade de seq³ência com a seq³ência nativa do gene yfck e codifica uma aminopeptidaso. Alternativamente, uma célula bacteriana gram-negativa é deficiente de um gene cromossómico presente nessa célula do tipo selvagem, em que o gene codifica uma aminopeptidase que compartilha pelo menos 80% de identidade de seq³ência com a seq³ência nativa da aminopeptidase b2324. Qualquer um destes tipos de células, quando compreender um ácido nucléico codificador de um polipeptídeo heterólogo, produz um polipeptídeo não destacado N-terminal quando é cultivado e o polipeptídeo é recuperado, com virtualmente nenhum polipeptídeo destacado N-terminal produzido como impureza. Por outro lado, é fornecido um método para a clivagem de aminoácido N-terminal de um polipeptídeo que compreende o contato do polipeptídeo com aminopeptidase que compartilha pelo menos 80% de identidade de seq³ência com a seq³ência nativa de aminopeptidase b2324.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32235001P | 2001-09-13 | 2001-09-13 | |
| PCT/US2002/029015 WO2003023030A2 (en) | 2001-09-13 | 2002-09-12 | Aminopeptidase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0212886A true BR0212886A (pt) | 2004-10-13 |
Family
ID=23254494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0212886-1A BR0212886A (pt) | 2001-09-13 | 2002-09-12 | Célula bacteriana, método para a produção de um polipeptìdeo heterólogo, método para a prevenção de clivagem, métodos para a clivagem e métodos para a produção de um polipeptìdeo clivado |
Country Status (22)
| Country | Link |
|---|---|
| US (3) | US6921659B2 (pt) |
| EP (1) | EP1432793B1 (pt) |
| JP (2) | JP4831932B2 (pt) |
| KR (2) | KR100956907B1 (pt) |
| CN (1) | CN100552023C (pt) |
| AT (1) | ATE440134T1 (pt) |
| AU (1) | AU2002326884B2 (pt) |
| BR (1) | BR0212886A (pt) |
| CA (1) | CA2460309C (pt) |
| CY (1) | CY1109903T1 (pt) |
| DE (1) | DE60233417D1 (pt) |
| DK (1) | DK1432793T3 (pt) |
| ES (1) | ES2329880T3 (pt) |
| HK (1) | HK1062695A1 (pt) |
| HU (3) | HU225430B1 (pt) |
| IL (2) | IL160658A0 (pt) |
| MX (1) | MXPA04002326A (pt) |
| NZ (2) | NZ531516A (pt) |
| PL (1) | PL206286B1 (pt) |
| PT (1) | PT1432793E (pt) |
| WO (1) | WO2003023030A2 (pt) |
| ZA (1) | ZA200401752B (pt) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MXPA04002326A (es) | 2001-09-13 | 2004-06-29 | Genentech Inc | Aminopeptidasa. |
| CN1954074B (zh) | 2004-03-11 | 2011-08-24 | 健泰科生物技术公司 | 多肽的制备方法 |
| EP2376619A4 (en) | 2008-12-15 | 2012-07-04 | Greenlight Biosciences Inc | METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS |
| CN105132387A (zh) * | 2008-12-22 | 2015-12-09 | 绿光生物科学公司 | 用于制造化合物的组合物和方法 |
| WO2011140516A2 (en) | 2010-05-07 | 2011-11-10 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through enzyme relocation |
| US8916358B2 (en) | 2010-08-31 | 2014-12-23 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| WO2013036787A2 (en) | 2011-09-09 | 2013-03-14 | Greenlight Biosciences, Inc. | Cell-free preparation of carbapenems |
| BR112016002494A2 (pt) | 2013-08-05 | 2017-09-05 | Greenlight Biosciences Inc | Proteí-nas construídas com um sítio de clivagem de protease, ácido nucléico, vetor, célula e processo de engenharia de uma proteína recombinante e de um grande número de variantes de ácidos nucleicos que codificam proteínas recombinantes |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| MX2017012665A (es) | 2015-03-30 | 2018-04-24 | Greenlight Biosciences Inc | Produccion de acido ribonucleico libre de celulas. |
| EP4293104A3 (en) | 2016-04-06 | 2024-04-24 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| CN106967658B (zh) * | 2017-02-23 | 2020-09-15 | 郑州大学 | 一种提高Fab抗体表达量的方法 |
| AU2018347405B2 (en) | 2017-10-11 | 2022-02-03 | Greenlight Biosciences, Inc. | Methods and compositions for nucleoside triphosphate and ribonucleic acid production |
| US11471497B1 (en) | 2019-03-13 | 2022-10-18 | David Gordon Bermudes | Copper chelation therapeutics |
| US10973908B1 (en) | 2020-05-14 | 2021-04-13 | David Gordon Bermudes | Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU591464B2 (en) * | 1984-08-16 | 1989-12-07 | Savient Pharmaceuticals, Inc. | Method of removing n-terminal amino acid residues using aeromonas aminopeptidases |
| WO1986007380A1 (en) * | 1985-06-04 | 1986-12-18 | Takeda Chemical Industries, Ltd. | Process for preparing polypeptide |
| US4865974A (en) * | 1985-09-20 | 1989-09-12 | Cetus Corporation | Bacterial methionine N-terminal peptidase |
| JPH01502320A (ja) | 1987-01-30 | 1989-08-17 | プレジデント アンド フェロウズ オブ ハーバード カレッジ | 大腸菌のペリプラズムプロテアーゼ変異体 |
| US4946783A (en) * | 1987-01-30 | 1990-08-07 | President And Fellows Of Harvard College | Periplasmic protease mutants of Escherichia coli |
| JPH01181796A (ja) * | 1988-01-14 | 1989-07-19 | Ajinomoto Co Inc | 組換えｄｎａ法を用いたポリペプチドの製造法 |
| JP2844870B2 (ja) * | 1989-07-21 | 1999-01-13 | 味の素株式会社 | 組み替えｄｎａ法によるポリペプチドの製造法 |
| US5508192A (en) * | 1990-11-09 | 1996-04-16 | Board Of Regents, The University Of Texas System | Bacterial host strains for producing proteolytically sensitive polypeptides |
| US5304472A (en) * | 1992-11-20 | 1994-04-19 | Genentech, Inc. | Method of controlling polypeptide production in bacterial cells |
| US5573923A (en) * | 1993-12-22 | 1996-11-12 | Eli Lilly And Company | Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum |
| US6127144A (en) * | 1993-12-23 | 2000-10-03 | Cangene Corporation | Method for expression of proteins in bacterial host cells |
| US5789199A (en) * | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5639635A (en) * | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| KR100369839B1 (ko) * | 1997-02-28 | 2003-06-12 | 주식회사 엘지생명과학 | 바실러스리케니포미스균주에서유래한아미노펩티다제,그의제조방법및이를이용한천연형단백질의제조방법 |
| CA2352046A1 (en) * | 1998-12-07 | 2000-06-15 | Novozymes A/S | Glucoamylases with n-terminal extensions |
| CA2370113A1 (en) * | 1999-04-30 | 2000-11-09 | Monsanto Company | Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase |
| KR100400638B1 (ko) | 2000-12-06 | 2003-10-08 | 주식회사 엘지생명과학 | 재조합 인간 성장호르몬 유사체의 분리방법 |
| MXPA04002326A (es) | 2001-09-13 | 2004-06-29 | Genentech Inc | Aminopeptidasa. |
-
2002
- 2002-09-12 MX MXPA04002326A patent/MXPA04002326A/es active IP Right Grant
- 2002-09-12 US US10/243,789 patent/US6921659B2/en not_active Expired - Lifetime
- 2002-09-12 PL PL368463A patent/PL206286B1/pl unknown
- 2002-09-12 WO PCT/US2002/029015 patent/WO2003023030A2/en active Application Filing
- 2002-09-12 HU HU0600309A patent/HU225430B1/hu unknown
- 2002-09-12 JP JP2003527095A patent/JP4831932B2/ja not_active Expired - Lifetime
- 2002-09-12 HU HU0401869A patent/HU225243B1/hu unknown
- 2002-09-12 AT AT02761635T patent/ATE440134T1/de active
- 2002-09-12 HU HU0600310A patent/HU225346B1/hu unknown
- 2002-09-12 IL IL16065802A patent/IL160658A0/xx unknown
- 2002-09-12 KR KR1020087030860A patent/KR100956907B1/ko active IP Right Grant
- 2002-09-12 DK DK02761635T patent/DK1432793T3/da active
- 2002-09-12 NZ NZ531516A patent/NZ531516A/xx not_active IP Right Cessation
- 2002-09-12 PT PT02761635T patent/PT1432793E/pt unknown
- 2002-09-12 DE DE60233417T patent/DE60233417D1/de not_active Expired - Lifetime
- 2002-09-12 ES ES02761635T patent/ES2329880T3/es not_active Expired - Lifetime
- 2002-09-12 KR KR1020047003708A patent/KR100954641B1/ko active IP Right Grant
- 2002-09-12 BR BR0212886-1A patent/BR0212886A/pt not_active Application Discontinuation
- 2002-09-12 CA CA2460309A patent/CA2460309C/en not_active Expired - Lifetime
- 2002-09-12 EP EP02761635A patent/EP1432793B1/en not_active Expired - Lifetime
- 2002-09-12 NZ NZ544964A patent/NZ544964A/en not_active IP Right Cessation
- 2002-09-12 CN CNB028179846A patent/CN100552023C/zh not_active Expired - Lifetime
- 2002-09-12 AU AU2002326884A patent/AU2002326884B2/en not_active Expired
-
2004
- 2004-03-01 IL IL160658A patent/IL160658A/en unknown
- 2004-03-03 ZA ZA2004/01752A patent/ZA200401752B/en unknown
- 2004-07-15 HK HK04105179.8A patent/HK1062695A1/xx not_active IP Right Cessation
-
2005
- 2005-05-16 US US11/131,035 patent/US7229787B2/en not_active Expired - Lifetime
-
2007
- 2007-04-27 US US11/741,510 patent/US7632658B2/en not_active Expired - Lifetime
-
2009
- 2009-10-26 CY CY20091101111T patent/CY1109903T1/el unknown
-
2011
- 2011-04-20 JP JP2011094093A patent/JP5372061B2/ja not_active Expired - Lifetime
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR0212886A (pt) | Célula bacteriana, método para a produção de um polipeptìdeo heterólogo, método para a prevenção de clivagem, métodos para a clivagem e métodos para a produção de um polipeptìdeo clivado | |
| NZ595496A (en) | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them | |
| ATE352626T1 (de) | Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren | |
| DK1025211T3 (da) | Hyaluronansyntasegen og anvendelser deraf | |
| PL2041276T3 (pl) | Sposób wytwarzania L-aminokwasów za pomocą mutantów genu gltA kodującego syntazę cytranianową | |
| ATE318890T1 (de) | Bakterielle wirtstämme | |
| MX2008016047A (es) | Plantas que tienen rasgos mejorados relacionados con el crecimiento y un metodo para obtener las mismas. | |
| NZ596764A (en) | N-glycan core beta-galactosyltransferase and uses thereof | |
| BR9802403A (pt) | Proteìna tendo atividade transglutaminase, dna, transformante, e, processo para produzir uma proteìna tendo uma atividade transglutaminase. | |
| EP1548107A4 (en) | CHROMOPROTEIN AND FLUOROPROTEINS | |
| DE60025467D1 (de) | Oxalacetat-hydrolase defiziente fungale wirtszellen | |
| ATE452184T1 (de) | Mikrobielle trypsinvarianten mit chymotrypsinaktivität und diese codierende nukleinsäuren | |
| ATE307205T1 (de) | Gefrorenes lebensmittel | |
| MX2021002250A (es) | Dominio de nucleasa novedoso y uso del mismo. | |
| PE32696A1 (es) | Gen codificante y procedimiento de expresion de una nueva enzima: ftalil amidasa | |
| MX2022009969A (es) | Variantes de mananasa. | |
| WO2005045045A3 (en) | Recombinant microorganism | |
| KR100391884B1 (en) | Random codon based mutagenesis using transposon | |
| ATE548448T1 (de) | D-aminosäure-oxidase aus arthrobacter protophormiae | |
| WO2023114831A3 (en) | Improvement of dnase1l3 serum half-life | |
| BR0017015A (pt) | Toxina estafilocócica modificada, gene codificante, vetor de expressão, célula e métodos de produção de polipeptìdeo, de separação e purificação da toxina e de, produção de vacina. | |
| WO2023034959A3 (en) | Polypeptides and methods for modifying nucleic acids | |
| MX2022009970A (es) | Variantes de mananasa. | |
| EA200100440A1 (ru) | Ген prv-1 и его применение | |
| MX2022009968A (es) | Variantes de mananasa. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B15K | Others concerning applications: alteration of classification |
Free format text: A CLASSIFICACAO ANTERIOR ERA: C12N 9/48 Ipc: C12N 9/48 (2006.01), A61K 38/27 (2006.01), A61P 5/ |
|
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
| B15K | Others concerning applications: alteration of classification |
Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 9/48 , A61K 38/27 , A61P 5/06 Ipc: C12N 1/20 (2006.01), C12N 1/21 (2006.01), C12P 21/ |
|
| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL |