BR0012627A - Processo para purificar o dna de plasmìdeo de células procarióticas mediante o emprego de filtração de fluxo tangencial - Google Patents

Processo para purificar o dna de plasmìdeo de células procarióticas mediante o emprego de filtração de fluxo tangencial

Info

Publication number
BR0012627A
BR0012627A BR0012627-6A BR0012627A BR0012627A BR 0012627 A BR0012627 A BR 0012627A BR 0012627 A BR0012627 A BR 0012627A BR 0012627 A BR0012627 A BR 0012627A
Authority
BR
Brazil
Prior art keywords
flow filtration
tangential flow
dna
purify
cells
Prior art date
Application number
BR0012627-6A
Other languages
English (en)
Inventor
Marjorie E Winkler
Michelle D Butler
Darien L Cohen
David Kahn
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of BR0012627A publication Critical patent/BR0012627A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/894—Manufacture, treatment, or detection of nanostructure having step or means utilizing biological growth
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/895—Manufacture, treatment, or detection of nanostructure having step or means utilizing chemical property
    • Y10S977/896—Chemical synthesis, e.g. chemical bonding or breaking
    • Y10S977/898—Enzymatic
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/91—Strengthening cell or tissue
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/912—Cancer cell repair
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/914—Protein engineering
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

"PROCESSO PARA PURIFICAR O DNA DE PLASMìDEO DE CéLULAS PROCARIóTICAS MEDIANTE O EMPREGO DE FILTRAçãO DE FLUXO TANGENCIAL". é descrito um processo para a purificação de DNA de plasmídeo de células procarióticas que os contêm. Esse processo compreende as etapas de: (a) digestão das células; (b) incubação das células durante aproximadamente 4 a 24 horas para efetuar a sua lise e solubilização, sem afetar a digestão enzimática do RNA; (c) remoção dos contaminantes de lisado das células, para se obter uma solução de DNA de plasmídeo; (d) filtração da solução através de um dispositivo de filtração de fluxo tangencial para se obter um material retido contendo o DNA de plasmídeo; e (e) coleta do material retido.
BR0012627-6A 1999-07-23 2000-07-21 Processo para purificar o dna de plasmìdeo de células procarióticas mediante o emprego de filtração de fluxo tangencial BR0012627A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14531699P 1999-07-23 1999-07-23
US16520699P 1999-11-12 1999-11-12
PCT/US2000/019963 WO2001007599A1 (en) 1999-07-23 2000-07-21 Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration

Publications (1)

Publication Number Publication Date
BR0012627A true BR0012627A (pt) 2002-04-09

Family

ID=26842848

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0012627-6A BR0012627A (pt) 1999-07-23 2000-07-21 Processo para purificar o dna de plasmìdeo de células procarióticas mediante o emprego de filtração de fluxo tangencial

Country Status (18)

Country Link
US (1) US6313285B1 (pt)
EP (1) EP1200578B1 (pt)
JP (1) JP2003505077A (pt)
KR (1) KR100669305B1 (pt)
CN (1) CN1378592A (pt)
AT (1) ATE264386T1 (pt)
BR (1) BR0012627A (pt)
CA (1) CA2378944A1 (pt)
DE (1) DE60009912T2 (pt)
DK (1) DK1200578T3 (pt)
ES (1) ES2218185T3 (pt)
HK (1) HK1048829A1 (pt)
HU (1) HUP0202295A3 (pt)
IL (1) IL147644A0 (pt)
NZ (1) NZ516641A (pt)
PL (1) PL364816A1 (pt)
PT (1) PT1200578E (pt)
WO (1) WO2001007599A1 (pt)

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EP1322337A2 (en) * 2000-09-25 2003-07-02 Board of Regents, The University of Texas System Pei : dna vector formulations for in vitro and in vivo gene delivery
US6995246B1 (en) * 2000-10-19 2006-02-07 Akzo Nobel N.V. Methods for removing suspended particles from soluble protein solutions
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
JP3547715B2 (ja) * 2001-03-06 2004-07-28 榮 新垣 ベクター精製用装置及び方法
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
ATE454469T1 (de) 2002-01-28 2010-01-15 Ambion Inc Für den nachweis von nukleinsäuren kompetente biologische rohderivate
AU2003267175A1 (en) 2002-09-13 2004-04-30 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
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US7790039B2 (en) * 2003-11-24 2010-09-07 Northwest Biotherapeutics, Inc. Tangential flow filtration devices and methods for stem cell enrichment
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
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US20080300397A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
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WO2009129524A2 (en) * 2008-04-18 2009-10-22 The General Hospital Corporation High-throughput plasmid dna preparation
TWI469992B (zh) * 2008-08-28 2015-01-21 Baxter Int 濃縮剪切靈敏生物聚合物的方法
US8211637B2 (en) * 2008-12-19 2012-07-03 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
DK3338765T3 (en) 2009-12-01 2019-03-04 Translate Bio Inc STEROID DERIVATIVE FOR THE SUPPLY OF MRNA IN HUMANGENETIC DISEASES
JP6219811B2 (ja) 2011-04-01 2017-10-25 イー・エム・デイー・ミリポア・コーポレイシヨン ナノファイバー含有複合材構造体
PL2717893T3 (pl) 2011-06-08 2019-12-31 Translate Bio, Inc. Kompozycje nanocząstek lipidowych i sposoby do dostarczania mRNA
WO2013185067A1 (en) 2012-06-08 2013-12-12 Shire Human Genetic Therapies, Inc. Nuclease resistant polynucleotides and uses thereof
ES2689523T3 (es) 2013-03-14 2018-11-14 Translate Bio, Inc. Composiciones a base de ARNM del gen CFTR y métodos y usos relacionados
BR112015022660A2 (pt) 2013-03-14 2017-10-31 Shire Human Genetic Therapies métodos para a purificação de rna mensageiro
CN103525866A (zh) * 2013-03-22 2014-01-22 人福医药集团股份公司 制备质粒的方法
EA201992208A1 (ru) 2013-10-22 2020-07-31 Транслейт Био, Инк. ЛЕЧЕНИЕ ФЕНИЛКЕТОНУРИИ С ПРИМЕНЕНИЕМ мРНК
EP3501605B1 (en) 2013-10-22 2023-06-28 Translate Bio, Inc. Mrna therapy for argininosuccinate synthetase deficiency
DK178664B1 (en) * 2013-10-25 2016-10-24 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
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CN110511927A (zh) 2014-04-25 2019-11-29 川斯勒佰尔公司 信使rna的纯化方法
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Also Published As

Publication number Publication date
JP2003505077A (ja) 2003-02-12
IL147644A0 (en) 2002-08-14
HUP0202295A2 (en) 2002-10-28
PL364816A1 (en) 2004-12-13
HK1048829A1 (zh) 2003-04-17
ES2218185T3 (es) 2004-11-16
CA2378944A1 (en) 2001-02-01
US6313285B1 (en) 2001-11-06
DE60009912T2 (de) 2005-04-14
WO2001007599A1 (en) 2001-02-01
ATE264386T1 (de) 2004-04-15
DK1200578T3 (da) 2004-07-26
CN1378592A (zh) 2002-11-06
EP1200578A1 (en) 2002-05-02
PT1200578E (pt) 2004-08-31
KR100669305B1 (ko) 2007-01-17
EP1200578B1 (en) 2004-04-14
KR20020019123A (ko) 2002-03-09
DE60009912D1 (de) 2004-05-19
HUP0202295A3 (en) 2005-01-28
NZ516641A (en) 2003-07-25

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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