ATE277175T1 - Ein verfahren für die plasmidreinigung in grossem massstab - Google Patents
Ein verfahren für die plasmidreinigung in grossem massstabInfo
- Publication number
- ATE277175T1 ATE277175T1 AT95927143T AT95927143T ATE277175T1 AT E277175 T1 ATE277175 T1 AT E277175T1 AT 95927143 T AT95927143 T AT 95927143T AT 95927143 T AT95927143 T AT 95927143T AT E277175 T1 ATE277175 T1 AT E277175T1
- Authority
- AT
- Austria
- Prior art keywords
- large scale
- cleaning
- disclosed
- plasmid dna
- plasmids
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 239000013612 plasmid Substances 0.000 title abstract 3
- 238000004140 cleaning Methods 0.000 title 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Detergent Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27557194A | 1994-07-15 | 1994-07-15 | |
| PCT/US1995/008749 WO1996002658A1 (en) | 1994-07-15 | 1995-07-11 | A method for large scale plasmid purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE277175T1 true ATE277175T1 (de) | 2004-10-15 |
Family
ID=23052893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95927143T ATE277175T1 (de) | 1994-07-15 | 1995-07-11 | Ein verfahren für die plasmidreinigung in grossem massstab |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0771355B1 (de) |
| JP (1) | JPH10503086A (de) |
| AT (1) | ATE277175T1 (de) |
| AU (1) | AU708798B2 (de) |
| CA (1) | CA2192342A1 (de) |
| DE (1) | DE69533552T2 (de) |
| ES (1) | ES2227557T3 (de) |
| WO (1) | WO1996002658A1 (de) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR003122A1 (es) * | 1995-05-19 | 1998-07-08 | Merck & Co Inc | Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso. |
| EP0880536B2 (de) | 1996-02-06 | 2015-08-05 | Roche Diagnostics GmbH | Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung |
| GB9602825D0 (en) * | 1996-02-12 | 1996-04-10 | Therexsys Ltd | Method of plasmid dna production and purification |
| US5981735A (en) | 1996-02-12 | 1999-11-09 | Cobra Therapeutics Limited | Method of plasmid DNA production and purification |
| US7026468B2 (en) | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US7807822B2 (en) * | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
| EP0853123A1 (de) * | 1997-01-10 | 1998-07-15 | Roche Diagnostics GmbH | Reinigung von DNA durch Cross-Flow-Filtration |
| FR2773818B1 (fr) * | 1998-01-21 | 2000-02-18 | Pasteur Merieux Serums Vacc | Procede et dispositif de lyse de bacteries |
| EP0964057B1 (de) | 1998-05-11 | 2004-12-22 | Tosoh Corporation | Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie |
| US6268492B1 (en) | 1998-08-10 | 2001-07-31 | Chiron Corporation | Methods for purifying non-chromosomal nucleic acid molecules from cells |
| US6664049B1 (en) | 1999-01-20 | 2003-12-16 | Aventis Pasteur S.A. | Method and device for cell lysis |
| CA2378944A1 (en) | 1999-07-23 | 2001-02-01 | Genentech, Inc. | Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration |
| US20020012990A1 (en) | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| US6406892B1 (en) | 2001-05-23 | 2002-06-18 | Bio-Rad Laboratories, Inc. | Acetate-free purification of plasmid DNA on hydroxyapatite |
| EP1321176A1 (de) * | 2001-12-18 | 2003-06-25 | Boehringer Ingelheim International GmbH | Verfahren und Vorrichtung zur Isolierung und Reinigung eines bestimmten Polynukleotides in industriellem Umfang |
| JP4723254B2 (ja) * | 2002-12-23 | 2011-07-13 | バイカル インコーポレイテッド | プラスミドｄｎａを精製するための方法 |
| US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
| EP1737945B1 (de) | 2004-04-19 | 2011-01-26 | Aventis Pharma S.A. | Verfahren zur herstellung von plasmid dns in pharmazeutischer qualität |
| EP1773387B1 (de) | 2004-06-25 | 2013-06-12 | Merial Ltd. | Maul- und klauenseuche-virengene-exprimierende avipox-rekombinante |
| EP1856262B1 (de) | 2005-01-31 | 2012-08-15 | Merck Sharp & Dohme Corp. | Upstream- und downstream-reinigungsverfahren zur massenfertigung von plasmider dna |
| EP2088196A1 (de) | 2008-02-08 | 2009-08-12 | Boehringer Ingelheim RCV GmbH & Co KG | Verfahren und Vorrichtungen zur Herstellung von Biomolekülen |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3928642A (en) * | 1975-01-22 | 1975-12-23 | Lilly Co Eli | Process for lysing mycelial waste |
| US4830969A (en) * | 1981-08-31 | 1989-05-16 | The Research Foundation Of State University Of New York | Process for the rapid and simple isolation of nucleic acids |
| US5256549A (en) * | 1986-03-28 | 1993-10-26 | Chiron Corporation | Purification of synthetic oligomers |
| ATE157012T1 (de) * | 1989-11-03 | 1997-09-15 | Univ Vanderbilt | Verfahren zur in vivo-verabreichung von funktionsfähigen fremden genen |
| WO1993024640A2 (en) * | 1992-06-04 | 1993-12-09 | The Regents Of The University Of California | Methods and compositions for in vivo gene therapy |
| US5834441A (en) * | 1993-09-13 | 1998-11-10 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Adeno-associated viral (AAV) liposomes and methods related thereto |
| AR003122A1 (es) * | 1995-05-19 | 1998-07-08 | Merck & Co Inc | Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso. |
-
1995
- 1995-07-11 WO PCT/US1995/008749 patent/WO1996002658A1/en active IP Right Grant
- 1995-07-11 CA CA002192342A patent/CA2192342A1/en not_active Abandoned
- 1995-07-11 EP EP95927143A patent/EP0771355B1/de not_active Expired - Lifetime
- 1995-07-11 AT AT95927143T patent/ATE277175T1/de not_active IP Right Cessation
- 1995-07-11 AU AU31262/95A patent/AU708798B2/en not_active Ceased
- 1995-07-11 JP JP8505121A patent/JPH10503086A/ja not_active Withdrawn
- 1995-07-11 DE DE69533552T patent/DE69533552T2/de not_active Expired - Lifetime
- 1995-07-11 ES ES95927143T patent/ES2227557T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0771355B1 (de) | 2004-09-22 |
| AU708798B2 (en) | 1999-08-12 |
| JPH10503086A (ja) | 1998-03-24 |
| AU3126295A (en) | 1996-02-16 |
| EP0771355A1 (de) | 1997-05-07 |
| WO1996002658A1 (en) | 1996-02-01 |
| CA2192342A1 (en) | 1996-02-01 |
| EP0771355A4 (de) | 1997-08-20 |
| ES2227557T3 (es) | 2005-04-01 |
| DE69533552D1 (de) | 2004-10-28 |
| DE69533552T2 (de) | 2005-09-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |