ATE454469T1 - Für den nachweis von nukleinsäuren kompetente biologische rohderivate - Google Patents
Für den nachweis von nukleinsäuren kompetente biologische rohderivateInfo
- Publication number
- ATE454469T1 ATE454469T1 AT03710760T AT03710760T ATE454469T1 AT E454469 T1 ATE454469 T1 AT E454469T1 AT 03710760 T AT03710760 T AT 03710760T AT 03710760 T AT03710760 T AT 03710760T AT E454469 T1 ATE454469 T1 AT E454469T1
- Authority
- AT
- Austria
- Prior art keywords
- detection
- competent
- derivatives
- nucleic acids
- biological raw
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35240202P | 2002-01-28 | 2002-01-28 | |
PCT/US2003/002439 WO2003064605A2 (en) | 2002-01-28 | 2003-01-28 | Crude biological derivatives competent for nucleic acid detection |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE454469T1 true ATE454469T1 (de) | 2010-01-15 |
Family
ID=27663090
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03710760T ATE454469T1 (de) | 2002-01-28 | 2003-01-28 | Für den nachweis von nukleinsäuren kompetente biologische rohderivate |
Country Status (6)
Country | Link |
---|---|
US (6) | US20030170617A1 (de) |
EP (4) | EP2290098A1 (de) |
AT (1) | ATE454469T1 (de) |
DE (1) | DE60330842D1 (de) |
HK (1) | HK1210627A1 (de) |
WO (1) | WO2003064605A2 (de) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ511680A (en) | 2001-05-14 | 2004-07-30 | Univ Waikato | Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases |
EP2290098A1 (de) | 2002-01-28 | 2011-03-02 | Ambion, Inc. | Herstellung biologische Rohderivaten unter Einzetsung Proteasen, geeignet für cDNA Synthese |
CA2518569C (en) * | 2003-03-10 | 2011-11-15 | Expression Pathology, Inc. | Liquid tissue preparation from histopathologically processed biological samples, tissues and cells |
CN1580283A (zh) * | 2003-08-13 | 2005-02-16 | 清华大学 | 一种检测核酸分子的方法 |
US20050208501A1 (en) | 2004-03-16 | 2005-09-22 | Ambion, Inc. | Process and reagents for extraction of RNA from fractionated blood leukocytes |
US20050239055A1 (en) * | 2004-04-22 | 2005-10-27 | Mariani Brian D | Enterovirus primers and probes |
US7575864B2 (en) * | 2004-05-27 | 2009-08-18 | E.I. Du Pont De Nemours And Company | Method for the direct detection of diagnostic RNA |
US20050277121A1 (en) * | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
SG178651A1 (en) * | 2004-06-17 | 2012-03-29 | Zygem Corp Ltd | Purification methods and uses thereof |
US20060188892A1 (en) * | 2005-02-18 | 2006-08-24 | Ambion, Inc. | Enzymatic digestion of tissue |
EP2049688B1 (de) * | 2006-08-01 | 2018-01-24 | Applied Biosystems, LLC | Nachweis von analyten und nukleinsäuren |
EP2147119B1 (de) * | 2007-05-03 | 2011-05-25 | Roche Diagnostics GmbH | mRNA-QUANTIFIZIERUNG EINER EINZELZELLE MITTELS ECHTZEIT-RT-PCR |
US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
WO2010012464A1 (en) | 2008-08-01 | 2010-02-04 | Roche Diagnostics Gmbh | Improved lysis and reverse transcription for mrna quantification |
EP2955233A1 (de) | 2008-12-19 | 2015-12-16 | Life Technologies Corporation | Proteinase K Inhibitore, Verfahren und Zusammensetzungen davon |
US9422542B2 (en) | 2010-02-26 | 2016-08-23 | Qiagen Gmbh | Process for parallel isolation and/or purification of RNA and DNA |
EP2559764A1 (de) | 2011-08-17 | 2013-02-20 | Qiagen GmbH | Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer |
CN113774163A (zh) * | 2014-04-28 | 2021-12-10 | 奎斯特诊断投资股份有限公司 | 肠道病毒和双埃柯病毒的分子检测 |
EP3368668B1 (de) | 2015-10-28 | 2023-11-29 | Silicon Valley Scientific, Inc. | Verfahren und vorrichtung zur codierung von zellularen räumlichen positionsinformationen |
US20170166956A1 (en) * | 2015-12-11 | 2017-06-15 | Shoreline Biome, Llc | Methods for DNA Preparation for Multiplex High Throughput Targeted Sequencing |
JP2019517780A (ja) | 2016-04-14 | 2019-06-27 | ティー2 バイオシステムズ,インコーポレーテッド | 複合試料における増幅のための方法及びシステム |
US10894990B2 (en) | 2016-05-17 | 2021-01-19 | Shoreline Biome, Llc | High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures |
US11231347B2 (en) | 2016-11-29 | 2022-01-25 | S2 Genomics, Inc. | Method and apparatus for processing tissue samples |
GB201803654D0 (en) | 2018-03-07 | 2018-04-25 | Arcticzymes As | Thermolabile proteinases |
CA3102089A1 (en) | 2018-06-01 | 2019-12-05 | S2 Genomics, Inc. | Method and apparatus for processing tissue samples |
CN108588224A (zh) * | 2018-06-14 | 2018-09-28 | 大连医科大学附属第医院 | 一种用于嗜铬细胞瘤/副神经节瘤早期诊断和术前评估的生物标志物及其应用 |
WO2021221109A1 (ja) * | 2020-04-30 | 2021-11-04 | タカラバイオ株式会社 | Rnaウイルスの検出方法 |
FR3116065A1 (fr) * | 2020-11-12 | 2022-05-13 | Innovative Diagnostics Genetics | Détection d’acides nucléiques dans un échantillon biologique |
CN113151398A (zh) * | 2021-05-07 | 2021-07-23 | 广州复能基因有限公司 | 外泌体中核酸分子的检测方法 |
Family Cites Families (60)
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EP0124317B1 (de) | 1983-04-27 | 1990-04-11 | Ici Americas Inc. | Prolin-Derivate |
GB8600263D0 (en) | 1985-01-22 | 1986-02-12 | Ici America Inc | Peptide derivatives |
US5055450A (en) | 1985-01-22 | 1991-10-08 | Ici Americas Inc. | Peptide derivatives |
US5194588A (en) | 1985-01-22 | 1993-03-16 | Ici Americas Inc. | Aminoalcohol intermediates for peptide derivatives |
US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
CA1317860C (en) | 1987-04-01 | 1993-05-18 | Daniel Louis Kacian | Techniques for preparing specimens for bacterial assays |
US5008245A (en) | 1988-10-27 | 1991-04-16 | University Of Kentucky Research Foundation | Novel peptidyl carbamate inhibitors of the enzyme elastase |
NO177716C (no) | 1989-06-09 | 1995-11-08 | Zeneca Ltd | Fremgangsmåte for fremstilling av terapeutisk aktive polypeptider, DNA-sekvens, ekspresjonsvektor og antistoff |
CA2021660A1 (en) | 1989-07-26 | 1991-01-27 | Philippe Bey | Peptidase inhibitors |
US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US4997932A (en) * | 1989-11-13 | 1991-03-05 | Boehringer Mannheim Corporation | Method and kit for purifying nucleic acids |
US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
US5284829A (en) | 1991-11-26 | 1994-02-08 | The Regents Of The University Of California | Synthetic tetrapeptides for the prevention of schistosome parasite infection |
MX9300492A (es) | 1992-01-29 | 1994-07-29 | Hitachi Chemical Co Ltd | Soporte inmovilizado de nucleotido y metodo para su produccion. |
AU681082B2 (en) | 1992-05-06 | 1997-08-21 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
EP0698082A4 (de) | 1993-05-06 | 1997-12-17 | Abbott Lab | Ein puffer zur direkten lyse und die feststellung der hiv-1 plasma virämie |
AU692237B2 (en) | 1994-02-03 | 1998-06-04 | Picower Institute For Medical Research, The | Compositions and methods for advanced glycosylation endproduct-mediated modulation of amyloidosis |
KR100238833B1 (ko) * | 1994-03-10 | 2000-03-02 | 다니엘 엘. 캐시앙 | 이온성 세제에 의한 효소 개재 반응의 억제를 방지하는 방법 |
US5712095A (en) * | 1994-06-16 | 1998-01-27 | Becton Dickinson And Company | Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors |
WO1996000228A1 (en) * | 1994-06-23 | 1996-01-04 | Dade International Inc. | Method for the rapid isolation of nucleic acid |
US5693467A (en) | 1995-05-19 | 1997-12-02 | The American Type Culture Collection | Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers |
WO1997035589A1 (en) * | 1996-03-26 | 1997-10-02 | Kopreski Michael S | Method enabling use of extracellular rna extracted from plasma or serum to detect, monitor or evaluate cancer |
US5939262A (en) | 1996-07-03 | 1999-08-17 | Ambion, Inc. | Ribonuclease resistant RNA preparation and utilization |
US5677124A (en) | 1996-07-03 | 1997-10-14 | Ambion, Inc. | Ribonuclease resistant viral RNA standards |
US6218105B1 (en) * | 1996-07-19 | 2001-04-17 | Kathleen S. Hall | High throughput papilloma virus in vitro infectivity assay |
US5871628A (en) | 1996-08-22 | 1999-02-16 | The University Of Texas System | Automatic sequencer/genotyper having extended spectral response |
ATE438719T1 (de) * | 1997-03-07 | 2009-08-15 | Siemens Healthcare Diagnostics | Spezifischer marker für prostatakrebs |
EP1023460A4 (de) * | 1997-06-25 | 2003-09-10 | Promega Corp | Verfahren zur isolierung von rna |
US6111096A (en) * | 1997-10-31 | 2000-08-29 | Bbi Bioseq, Inc. | Nucleic acid isolation and purification |
EP1032595B1 (de) | 1997-11-18 | 2011-05-18 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Nukleinsäure involviert im responder phenotyp und deren verwendung |
WO1999037668A1 (de) | 1998-01-26 | 1999-07-29 | Basf Aktiengesellschaft | Thrombininhibitoren |
US6583301B1 (en) | 1998-04-08 | 2003-06-24 | Celltech R & D Limited | Lipids |
US6204375B1 (en) | 1998-07-31 | 2001-03-20 | Ambion, Inc. | Methods and reagents for preserving RNA in cell and tissue samples |
AU755499B2 (en) | 1998-09-18 | 2002-12-12 | Micromet Ag | DNA amplification of a single cell |
US6777210B1 (en) * | 1998-09-24 | 2004-08-17 | Ambion, Inc. | Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1 |
US6265165B1 (en) * | 1998-11-12 | 2001-07-24 | The Regents Of The University Of California | Methods for EST-specific full length cDNA cloning |
WO2000034504A2 (en) | 1998-12-10 | 2000-06-15 | Genetic Vectors, Inc. | Method of protein removal |
DE19916534A1 (de) * | 1999-04-13 | 2000-10-19 | Gsf Forschungszentrum Umwelt | RNA-Isolierungs-Kit |
CA2378944A1 (en) | 1999-07-23 | 2001-02-01 | Genentech, Inc. | Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration |
US7264932B2 (en) | 1999-09-24 | 2007-09-04 | Applera Corporation | Nuclease inhibitor cocktail |
EP1220944B1 (de) * | 1999-09-24 | 2010-09-15 | Ambion, Inc. | Cocktail von nukleaseinhibitoren |
WO2001042507A1 (en) | 1999-12-09 | 2001-06-14 | Advanced Research & Technology Institute | Fluorescent in situ rt-pcr |
US6316608B1 (en) | 2000-03-20 | 2001-11-13 | Incyte Genomics, Inc. | Combined polynucleotide sequence as discrete assay endpoints |
US7001724B1 (en) | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
JP2004523229A (ja) | 2001-01-16 | 2004-08-05 | インヴィトロジェン コーポレーション | Rna単離のための試薬 |
US20020172972A1 (en) | 2001-05-15 | 2002-11-21 | President And Fellows Of Harvard College | Use of a selectively inactivatable enzyme to digest contaminating nucleic acid |
US7229810B2 (en) | 2001-06-28 | 2007-06-12 | Mountain View Pharmaceuticals, Inc. | Polymer conjugates of proteinases |
EP2290098A1 (de) | 2002-01-28 | 2011-03-02 | Ambion, Inc. | Herstellung biologische Rohderivaten unter Einzetsung Proteasen, geeignet für cDNA Synthese |
US20040038213A1 (en) | 2002-08-06 | 2004-02-26 | Kwon Jai W. | Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy |
US7214484B2 (en) | 2002-12-17 | 2007-05-08 | Sigma-Aldrich Co. | Compositions and methods for nucleic acid extraction from biological samples |
WO2004076640A2 (en) | 2003-02-25 | 2004-09-10 | Ambion, Inc. | Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same |
US7067298B2 (en) | 2003-03-31 | 2006-06-27 | Ambion, Inc. | Compositions and methods of using a synthetic Dnase I |
CN1798838B (zh) | 2003-04-04 | 2012-01-04 | 洛桑大学 | 一种蛋白酶的抑制剂蛋白及其用途 |
US20050069953A1 (en) | 2003-09-30 | 2005-03-31 | Ye Fang | Assay solution compositions and methods for GPCR arrays |
US20050277121A1 (en) | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
WO2006047482A2 (en) | 2004-10-22 | 2006-05-04 | Redpath Integrated Pathology, Inc. | Method for determining the diagnosis, malignant potential, and biologic behavior of pancreatic cysts using cyst aspirate |
US20060188892A1 (en) | 2005-02-18 | 2006-08-24 | Ambion, Inc. | Enzymatic digestion of tissue |
US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
EP2955233A1 (de) | 2008-12-19 | 2015-12-16 | Life Technologies Corporation | Proteinase K Inhibitore, Verfahren und Zusammensetzungen davon |
-
2003
- 2003-01-28 EP EP10182642A patent/EP2290098A1/de not_active Withdrawn
- 2003-01-28 EP EP09180993.9A patent/EP2163652B2/de not_active Expired - Lifetime
- 2003-01-28 EP EP14197615.9A patent/EP2902501B1/de not_active Expired - Lifetime
- 2003-01-28 DE DE60330842T patent/DE60330842D1/de not_active Expired - Lifetime
- 2003-01-28 EP EP03710760A patent/EP1476573B1/de not_active Revoked
- 2003-01-28 US US10/352,806 patent/US20030170617A1/en not_active Abandoned
- 2003-01-28 WO PCT/US2003/002439 patent/WO2003064605A2/en not_active Application Discontinuation
- 2003-01-28 AT AT03710760T patent/ATE454469T1/de not_active IP Right Cessation
-
2012
- 2012-05-03 US US13/463,624 patent/US9611497B2/en not_active Expired - Lifetime
- 2012-09-14 US US13/617,840 patent/US20130143275A1/en not_active Abandoned
-
2015
- 2015-11-17 HK HK15111314.9A patent/HK1210627A1/xx not_active IP Right Cessation
-
2017
- 2017-03-22 US US15/466,824 patent/US10011831B2/en not_active Expired - Lifetime
-
2018
- 2018-06-06 US US16/001,279 patent/US10233444B2/en not_active Expired - Lifetime
-
2019
- 2019-03-08 US US16/297,492 patent/US10590411B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US9611497B2 (en) | 2017-04-04 |
EP2902501A1 (de) | 2015-08-05 |
US20130143275A1 (en) | 2013-06-06 |
DE60330842D1 (de) | 2010-02-25 |
US20030170617A1 (en) | 2003-09-11 |
EP2902501B1 (de) | 2020-04-15 |
US10233444B2 (en) | 2019-03-19 |
US20170218359A1 (en) | 2017-08-03 |
US10590411B2 (en) | 2020-03-17 |
EP1476573B1 (de) | 2010-01-06 |
EP2163652B2 (de) | 2023-05-31 |
US20180340175A1 (en) | 2018-11-29 |
HK1210627A1 (en) | 2016-04-29 |
US20120322112A1 (en) | 2012-12-20 |
WO2003064605A3 (en) | 2004-02-19 |
US20190270988A1 (en) | 2019-09-05 |
WO2003064605A2 (en) | 2003-08-07 |
EP2163652A1 (de) | 2010-03-17 |
EP2290098A1 (de) | 2011-03-02 |
EP1476573A2 (de) | 2004-11-17 |
EP1476573A4 (de) | 2005-12-14 |
EP2163652B1 (de) | 2014-12-24 |
US10011831B2 (en) | 2018-07-03 |
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