BR9807061A - Purificação de dna em uma centrìfuga de fluxo contìnuo - Google Patents
Purificação de dna em uma centrìfuga de fluxo contìnuoInfo
- Publication number
- BR9807061A BR9807061A BR9807061-4A BR9807061A BR9807061A BR 9807061 A BR9807061 A BR 9807061A BR 9807061 A BR9807061 A BR 9807061A BR 9807061 A BR9807061 A BR 9807061A
- Authority
- BR
- Brazil
- Prior art keywords
- dna
- continuous flow
- extrachromosomal
- relates
- dna purification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
- B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2221/00—Applications of separation devices
- B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Patente de Invenção: <B>"PURIFICAçãO DE DNA EM UMA CENTRìFUGA DE FLUXO CONTìNUO"<D>. A presente invenção refere-se um método de purificação de DNA extracromossomal passando-se um DNA extracromossomal e fluído contendo outros componentes celulares através de uma centrífuga de fluxo contínuo sob as condições dadas, resultando em separação do DNA extracromossomal dos outros componentes celulares, tal que o DNA extracromossomal purificado seja obtido. A invenção ainda refere-se ao uso do DNA extracromossomal purificado para clonagem, transformação, transfecção e microinjeção em células, para uso em processos de terapia de gene, vacinação de DNA e/ou para reação de cadeia de polimerase (PCR). A invenção finalmente refere-se ao uso de uma centrífuga para purificação de DNA extracromossomal.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97100330 | 1997-01-10 | ||
PCT/EP1998/000104 WO1998030686A2 (de) | 1997-01-10 | 1998-01-09 | Reinigung von dna in einer durchflusszentrifuge |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9807061A true BR9807061A (pt) | 2000-05-30 |
Family
ID=8226361
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9807061-4A BR9807061A (pt) | 1997-01-10 | 1998-01-09 | Purificação de dna em uma centrìfuga de fluxo contìnuo |
Country Status (10)
Country | Link |
---|---|
US (1) | US20020015982A1 (pt) |
EP (1) | EP0973883A2 (pt) |
JP (1) | JP2001512963A (pt) |
KR (1) | KR100337046B1 (pt) |
CN (1) | CN1142273C (pt) |
AU (1) | AU720911B2 (pt) |
BR (1) | BR9807061A (pt) |
CA (1) | CA2277468C (pt) |
TR (1) | TR199901606T2 (pt) |
WO (1) | WO1998030686A2 (pt) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20020028387A (ko) * | 2000-10-09 | 2002-04-17 | 박제철 | 대량의 동물조직으로부터 미토콘드리아 디.엔.에이의순수분리 방법 |
KR100930858B1 (ko) | 2008-02-11 | 2009-12-11 | 전북대학교산학협력단 | 진핵세포 형질전환을 위한 유전자 전달장치 |
WO2020160087A1 (en) * | 2019-01-29 | 2020-08-06 | Flagship Pioneering Innovations V, Inc. | Methods of separating long polynucleotides from a composition |
CN111073885A (zh) * | 2019-12-31 | 2020-04-28 | 江苏耀海生物制药有限公司 | 一种应用于双链dna片段的纯化方法 |
CN114082224A (zh) * | 2020-08-24 | 2022-02-25 | 重庆精准生物技术有限公司 | 适用于大规模质粒dna生产的纯化方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5114589A (en) * | 1991-01-17 | 1992-05-19 | The United States Government As Represented By The Secretary Of The Department Of Health And Human Services | Type-XLL cross-axis synchronous flow-through coil planet centrifuge for separation of biopolymers |
-
1998
- 1998-01-09 KR KR1019997006157A patent/KR100337046B1/ko not_active IP Right Cessation
- 1998-01-09 TR TR1999/01606T patent/TR199901606T2/xx unknown
- 1998-01-09 BR BR9807061-4A patent/BR9807061A/pt not_active IP Right Cessation
- 1998-01-09 US US09/341,367 patent/US20020015982A1/en not_active Abandoned
- 1998-01-09 WO PCT/EP1998/000104 patent/WO1998030686A2/de not_active Application Discontinuation
- 1998-01-09 CA CA002277468A patent/CA2277468C/en not_active Expired - Fee Related
- 1998-01-09 AU AU58627/98A patent/AU720911B2/en not_active Ceased
- 1998-01-09 EP EP98901955A patent/EP0973883A2/de not_active Withdrawn
- 1998-01-09 CN CNB988017636A patent/CN1142273C/zh not_active Expired - Fee Related
- 1998-01-09 JP JP53055598A patent/JP2001512963A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
WO1998030686A3 (de) | 1998-09-11 |
CN1142273C (zh) | 2004-03-17 |
CN1243542A (zh) | 2000-02-02 |
AU5862798A (en) | 1998-08-03 |
KR100337046B1 (ko) | 2002-05-16 |
CA2277468C (en) | 2003-03-25 |
CA2277468A1 (en) | 1998-07-16 |
WO1998030686A2 (de) | 1998-07-16 |
US20020015982A1 (en) | 2002-02-07 |
AU720911B2 (en) | 2000-06-15 |
EP0973883A2 (de) | 2000-01-26 |
KR20000069944A (ko) | 2000-11-25 |
JP2001512963A (ja) | 2001-08-28 |
TR199901606T2 (xx) | 1999-11-22 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1937 DE 19/02/2008 |