ZA901476B - A yeast processing system - Google Patents

A yeast processing system

Info

Publication number: ZA901476B
Authority: ZA; South Africa
Prior art keywords: terminal end; polypeptide; heterologous protein; yeast; presentation
Prior art date: 1989-03-03

Application number

ZA901476A

Other languages

English (en)

Inventor

Soren Bjorn

Bjorn Soren

Kjeld Norris

Norris Kjeld

Fanny Norris

Norris Fanny

Original Assignee

Novo Nordisk As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-03-03

Filing date

1990-02-27

Publication date

1990-11-28

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26065137&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ZA901476(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1989-10-06 Priority claimed from DK494189A external-priority patent/DK494189D0/da

1990-02-27 Application filed by Novo Nordisk As filed Critical Novo Nordisk As

1990-11-28 Publication of ZA901476B publication Critical patent/ZA901476B/xx

Links

240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
108090000623 proteins and genes Proteins 0.000 abstract 4
102000004169 proteins and genes Human genes 0.000 abstract 4
108010076504 Protein Sorting Signals Proteins 0.000 abstract 3
229920001184 polypeptide Polymers 0.000 abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 3
102000004196 processed proteins & peptides Human genes 0.000 abstract 3
150000001413 amino acids Chemical group 0.000 abstract 2
210000004899 c-terminal region Anatomy 0.000 abstract 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
108010039627 Aprotinin Proteins 0.000 abstract 1
102000004877 Insulin Human genes 0.000 abstract 1
108090001061 Insulin Proteins 0.000 abstract 1
229960004405 aprotinin Drugs 0.000 abstract 1
230000004927 fusion Effects 0.000 abstract 1
ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 abstract 1
229940125396 insulin Drugs 0.000 abstract 1
239000002243 precursor Substances 0.000 abstract 1
230000006337 proteolytic cleavage Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Mycology (AREA)
Endocrinology (AREA)
Toxicology (AREA)
Diabetes (AREA)
Botany (AREA)
Tropical Medicine & Parasitology (AREA)
Virology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Processing Of Solid Wastes (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Silver Salt Photography Or Processing Solution Therefor (AREA)
Chemical Or Physical Treatment Of Fibers (AREA)
Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

ZA901476A 1989-03-03 1990-02-27 A yeast processing system ZA901476B (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
DK105489A DK105489D0 (da)	1989-03-03	1989-03-03	Polypeptid
DK494189A DK494189D0 (da)	1989-10-06	1989-10-06	Polypeptid
PCT/DK1990/000058 WO1990010075A1 (en)	1989-03-03	1990-03-01	Yeast processing system comprising a negatively charged amino acid adjacent to the processing site

Publications (1)

Publication Number	Publication Date
ZA901476B true ZA901476B (en)	1990-11-28

Family

ID=26065137

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ZA901476A ZA901476B (en)	1989-03-03	1990-02-27	A yeast processing system

Country Status (23)

Country	Link
US (3)	US5395922A (hu)
EP (1)	EP0461165B2 (hu)
JP (1)	JP2609367B2 (hu)
KR (1)	KR970005928B1 (hu)
AT (1)	ATE110414T1 (hu)
AU (1)	AU624694B2 (hu)
CA (1)	CA2050336C (hu)
CZ (1)	CZ285239B6 (hu)
DD (1)	DD296965A5 (hu)
DE (1)	DE69011853T3 (hu)
DK (2)	DK105489D0 (hu)
ES (1)	ES2062514T5 (hu)
FI (1)	FI104985B (hu)
HU (2)	HU215538B (hu)
IE (1)	IE66469B1 (hu)
IL (1)	IL93609A (hu)
NO (2)	NO304236B1 (hu)
NZ (1)	NZ232742A (hu)
PL (1)	PL163532B1 (hu)
RU (1)	RU2194758C2 (hu)
UA (1)	UA27706C2 (hu)
WO (1)	WO1990010075A1 (hu)
ZA (1)	ZA901476B (hu)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
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US5591603A (en) *	1987-08-28	1997-01-07	Novo Nordisk A/S	Process for preparing aprotinin and aprotinin analogs in yeast cells
DK105489D0 (da) *	1989-03-03	1989-03-03	Novo Nordisk As	Polypeptid
AU647025B2 (en) *	1989-05-19	1994-03-17	Biotechnology Research And Development Corporation	Fusion proteins having an in vivo post-translational modification site and methods of manufacture and purification
GB9015825D0 (en) *	1990-07-18	1990-09-05	Ciba Geigy Ag	In vitro processing of fusion proteins
DK300090D0 (da) *	1990-12-19	1990-12-19	Novo Nordisk As	Fremgangsmaade til fremstilling af leadersekvenser
GB9106185D0 (en) *	1991-03-22	1991-05-08	Wellcome Found	Biological control agents
FR2686899B1 (fr) *	1992-01-31	1995-09-01	Rhone Poulenc Rorer Sa	Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
FI92601C (fi) *	1992-03-11	1994-12-12	Marja Makarow	Menetelmä hyötyproteiinien erittämiseksi hiivoista
US5521086A (en) *	1993-09-16	1996-05-28	Cephalon, Inc.	Secretion sequence for the production of a heterologous protein in yeast
DE4417353A1 (de) *	1994-05-18	1996-01-25	Bayer Ag	Verfahren zur Herstellung von rekombinantem Aprotinin und rekombinanten Aprotinin Varianten mit der natürlichen N-terminlaen Sequenz
US6500645B1 (en)	1994-06-17	2002-12-31	Novo Nordisk A/S	N-terminally extended proteins expressed in yeast
IL114160A (en) *	1994-06-17	2006-12-31	Novo Nordisk As	Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast
US5861267A (en) *	1995-05-01	1999-01-19	Vertex Pharmaceuticals Incorporated	Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
ZA9610456B (en) *	1995-12-20	1997-06-20	Novo Nordisk As	N-terminally extended proteins expressed in yeast
US5851800A (en) *	1996-05-14	1998-12-22	Pharmacia & Upjohn Ab	Process for producing a protein
DE19629982A1 (de)	1996-07-25	1998-01-29	Bayer Ag	Aprotinin-Varianten mit verbesserten Eigenschaften
EP1365028B1 (en)	1996-12-13	2009-03-18	Novartis Vaccines and Diagnostics, Inc.	Method for expression of PDGF or IGF proteins in yeast
JP4187796B2 (ja) *	1996-12-20	2008-11-26	ノボノルディスクアクティーゼルスカブ	酵母で発現されたｎ末端伸長タンパク質
EP0954589A1 (en) *	1997-01-24	1999-11-10	Novo Nordisk A/S	Synthetic leader peptide sequences
US6046000A (en) *	1997-11-07	2000-04-04	Millennium Biotherapeutics, Inc.	Method for identifying genes encoding signal sequences
KR20010034305A (ko)	1998-01-23	2001-04-25	한센 핀 베네드	효모에서 원하는 폴리펩티드를 만드는 방법
AU1995701A (en)	1999-12-29	2001-07-16	Novo Nordisk A/S	Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US20050100991A1 (en) *	2001-04-12	2005-05-12	Human Genome Sciences, Inc.	Albumin fusion proteins
US20030171267A1 (en) *	2000-04-12	2003-09-11	Rosen Craig A.	Albumin fusion proteins
US7507413B2 (en) *	2001-04-12	2009-03-24	Human Genome Sciences, Inc.	Albumin fusion proteins
US20050054051A1 (en) *	2001-04-12	2005-03-10	Human Genome Sciences, Inc.	Albumin fusion proteins
US7595172B2 (en)	2001-07-24	2009-09-29	Novo Nordisk A/S	Method for making acylated polypeptides
EP1997829A1 (en)	2001-12-21	2008-12-03	Human Genome Sciences, Inc.	Albumin fusion proteins
AU2002364587A1 (en)	2001-12-21	2003-07-30	Human Genome Sciences, Inc.	Albumin fusion proteins
CA2925807C (en)	2002-09-13	2020-09-15	Cornell Research Foundation, Inc.	Using mutations to improve aspergillus phytases
EP1594530A4 (en)	2003-01-22	2006-10-11	Human Genome Sciences Inc	HYBRID PROTEINS OF ALBUMIN
WO2005047508A1 (en)	2003-11-14	2005-05-26	Novo Nordisk A/S	Processes for making acylated insulin
EP1692168B1 (en)	2003-12-03	2011-07-20	Novo Nordisk A/S	Single-chain insulin
US9056921B2 (en)	2005-08-16	2015-06-16	Novo Nordisk A/S	Method for making mature insulin polypeptides
ES2387955T3 (es)	2006-02-27	2012-10-04	Novo Nordisk A/S	Derivados de insulina
US20080026376A1 (en)	2006-07-11	2008-01-31	Huaming Wang	KEX2 cleavage regions of recombinant fusion proteins
US8198046B2 (en)	2006-07-11	2012-06-12	Danisco Us Inc.	KEX2 cleavage regions of recombinant fusion proteins
BRPI0717098B8 (pt)	2006-09-22	2021-05-25	Novo Nordisk As	análogos de insulina humana resistentes à protease, composição farmacêutica e processo para sua preparação
JP5503968B2 (ja)	2006-09-27	2014-05-28	ノボ・ノルデイスク・エー／エス	成熟インスリンポリペプチドの作製方法
WO2008062010A2 (en)	2006-11-22	2008-05-29	Novo Nordisk A/S	Method for making activated carboxypeptidases
WO2009010428A1 (en)	2007-07-16	2009-01-22	Novo Nordisk A/S	Protease stabilized, pegylated insulin analogues
US8575096B2 (en)	2007-08-13	2013-11-05	Novo Nordisk A/S	Rapid acting insulin analogues
EP2708554A1 (en)	2007-08-15	2014-03-19	Novo Nordisk A/S	Insulin analogues with an acyl and alkylene glycol moiety
EP2178910B1 (en)	2007-08-15	2014-10-08	Novo Nordisk A/S	Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids
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KR20110004366A (ko)	2008-03-18	2011-01-13	노보 노르디스크 에이/에스	프로테아제 안정화되고, 아실화된 인슐린 유사체
WO2009121884A1 (en)	2008-04-01	2009-10-08	Novo Nordisk A/S	Insulin albumin conjugates
WO2011089170A2 (en)	2010-01-22	2011-07-28	Novo Nordisk A/S	Process for preparing fgf-21 with low degree of o-glycosylation
AU2010223154B2 (en) *	2009-03-12	2014-10-09	Bigtec Private Limited	A polynucleotide and polypeptide sequence and methods thereof
PL2612677T3 (pl)	2009-06-26	2015-10-30	Novo Nordisk As	Preparat zawierający insulinę, nikotynamid i argininę
US8815541B2 (en)	2009-11-25	2014-08-26	Novo Nordisk A/S	Method for making polypeptides
WO2011161124A1 (en)	2010-06-23	2011-12-29	Novo Nordisk A/S	Insulin analogues containing additional disulfide bonds
CA2802510A1 (en)	2010-06-23	2011-12-29	Novo Nordisk A/S	Insulin derivatives containing additional disulfide bonds
EP2585483A1 (en)	2010-06-23	2013-05-01	Novo Nordisk A/S	Human insulin containing additional disulfide bonds
JP2013545782A (ja)	2010-12-14	2013-12-26	ノヴォノルディスクアー／エス	長時間作用型インスリンと組み合わせた速効型インスリン
EP2651446A1 (en)	2010-12-14	2013-10-23	Novo Nordisk A/S	Preparation comprising insulin, nicotinamide and an amino acid
CN103596584B (zh)	2011-06-15	2016-12-14	诺沃—诺迪斯克有限公司	多取代的胰岛素
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WO2013186138A1 (en)	2012-06-14	2013-12-19	Novo Nordisk A/S	Preparation comprising insulin, nicotinamide and arginine
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1989
- 1989-03-03 DK DK105489A patent/DK105489D0/da not_active Application Discontinuation
1990
- 1990-02-27 ZA ZA901476A patent/ZA901476B/xx unknown
- 1990-03-01 AT AT90904258T patent/ATE110414T1/de not_active IP Right Cessation
- 1990-03-01 JP JP2504527A patent/JP2609367B2/ja not_active Expired - Lifetime
- 1990-03-01 DK DK90904258.2T patent/DK0461165T3/da active
- 1990-03-01 WO PCT/DK1990/000058 patent/WO1990010075A1/en active IP Right Grant
- 1990-03-01 CA CA002050336A patent/CA2050336C/en not_active Expired - Fee Related
- 1990-03-01 KR KR1019910701029A patent/KR970005928B1/ko not_active IP Right Cessation
- 1990-03-01 AU AU52612/90A patent/AU624694B2/en not_active Ceased
- 1990-03-01 NZ NZ232742A patent/NZ232742A/xx unknown
- 1990-03-01 EP EP90904258A patent/EP0461165B2/en not_active Expired - Lifetime
- 1990-03-01 DE DE69011853T patent/DE69011853T3/de not_active Expired - Lifetime
- 1990-03-01 UA UA5010019A patent/UA27706C2/uk unknown
- 1990-03-01 HU HU902376A patent/HU215538B/hu not_active IP Right Cessation
- 1990-03-01 ES ES90904258T patent/ES2062514T5/es not_active Expired - Lifetime
- 1990-03-01 RU SU5010019/13A patent/RU2194758C2/ru not_active IP Right Cessation
- 1990-03-02 CZ CS901039A patent/CZ285239B6/cs not_active IP Right Cessation
- 1990-03-02 IE IE74990A patent/IE66469B1/en not_active IP Right Cessation
- 1990-03-02 DD DD90338353A patent/DD296965A5/de unknown
- 1990-03-02 IL IL9360990A patent/IL93609A/en not_active IP Right Cessation
- 1990-03-02 PL PL90284146A patent/PL163532B1/pl not_active IP Right Cessation
1991
- 1991-09-02 FI FI914125A patent/FI104985B/fi not_active IP Right Cessation
- 1991-09-02 NO NO913427A patent/NO304236B1/no unknown
1994
- 1994-02-15 US US08/196,887 patent/US5395922A/en not_active Expired - Lifetime
1995
- 1995-03-02 US US08/397,597 patent/US5514585A/en not_active Expired - Lifetime
- 1995-03-02 US US08/397,595 patent/US5510249A/en not_active Expired - Lifetime
- 1995-06-29 HU HU95P/P00592P patent/HU211600A9/hu unknown
1997
- 1997-10-23 NO NO974899A patent/NO974899D0/no unknown

Also Published As

Publication number	Publication date
DE69011853T3 (de)	1998-06-25
JPH04504846A (ja)	1992-08-27
US5514585A (en)	1996-05-07
IE66469B1 (en)	1995-12-27
CZ103990A3 (cs)	1999-03-17
DE69011853D1 (de)	1994-09-29
JP2609367B2 (ja)	1997-05-14
DK0461165T3 (da)	1994-11-21
NO974899L (no)	1997-10-23
US5510249A (en)	1996-04-23
UA27706C2 (uk)	2000-10-16
EP0461165A1 (en)	1991-12-18
HUT58370A (en)	1992-02-28
CZ285239B6 (cs)	1999-06-16
CA2050336C (en)	2000-01-18
NZ232742A (en)	1991-10-25
NO974899D0 (no)	1997-10-23
FI104985B (fi)	2000-05-15
DE69011853T2 (de)	1994-12-15
CA2050336A1 (en)	1990-09-04
KR970005928B1 (ko)	1997-04-22
HU215538B (hu)	1999-01-28
DK105489D0 (da)	1989-03-03
ATE110414T1 (de)	1994-09-15
AU5261290A (en)	1990-09-26
PL163532B1 (pl)	1994-04-29
DD296965A5 (de)	1991-12-19
HU211600A9 (en)	1995-12-28
RU2194758C2 (ru)	2002-12-20
IE900749L (en)	1990-09-03
ES2062514T3 (es)	1994-12-16
US5395922A (en)	1995-03-07
KR920701452A (ko)	1992-08-11
EP0461165B2 (en)	1998-03-25
FI914125A0 (fi)	1991-09-02
EP0461165B1 (en)	1994-08-24
IL93609A (en)	2001-11-25
WO1990010075A1 (en)	1990-09-07
NO304236B1 (no)	1998-11-16
HU902376D0 (en)	1991-11-28
IL93609A0 (en)	1990-12-23
ES2062514T5 (es)	1998-06-01
NO913427D0 (no)	1991-09-02
AU624694B2 (en)	1992-06-18
NO913427L (no)	1991-11-01

Publication	Publication Date	Title
IL93609A0 (en)	1990-12-23	Yeast processing system
ATE160587T1 (de)	1997-12-15	Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung
EP0307592A3 (de)	1990-06-06	Varianten des pankreatischen Rinder-Trypsininhibitors, deren Herstellung und Verwendung
HUT54213A (en)	1991-01-28	Process for the enzymatic removal of n-terminal sequence of proteins
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AU1003888A (en)	1988-09-01	Pancreatic secretory trypsin inhibitor and variants thereof produced by a recombinant host, process, expression vector and recombinant host therefore and pharmaceutical use thereof
IL76107A (en)	1991-03-10	Method of removing n-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby
DK114788A (da)	1988-09-05	Fremgangsmaade til fremstilling af et fysiologisk aktivt peptid
FI961238A (fi)	1996-05-10	Erityssekvenssi heterologisen proteiinin tuottamiseksi hiivassa
EP0210279A4 (en)	1987-06-19	STABILIZED HUMAN PROUROKINASE.
AU7873798A (en)	1998-07-17	N-terminally extended proteins expressed in yeast
AU7166891A (en)	1991-08-21	Method of purifying recombinant polypeptides
CA2198966A1 (en)	1997-09-04	Method for cleaving chimeric protein using processing enzyme
Salokangas et al.	1971	Bovine proinsulin: amino acid sequence of the C‐peptide isolated from pancreas
AU651830B2 (en)	1994-08-04	In vitro processing of fusion proteins
Bjorn et al.	1995	Yeast processing system