WO2016063986A1 - 網膜組織の製造方法 - Google Patents
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Definitions
- the present invention relates to a method for producing retinal cells or retinal tissue from pluripotent stem cells.
- a method of producing neural tissue such as retinal tissue from pluripotent stem cells uniform aggregates of pluripotent stem cells are formed in a serum-free medium, and after this is cultured in suspension, it is used for differentiation induction.
- a method for producing neural tissue by culturing suspension in culture medium in the presence of a differentiation-inducing factor as appropriate and inducing differentiation from pluripotent stem cells to the desired neural cells has been reported. (Patent Document 1 and Non-Patent Document 1).
- Non-patent Document 2 and Patent Document 2 For example, a method of obtaining multi-layered retinal tissue from pluripotent stem cells (Non-patent Document 2 and Patent Document 2), forming uniform pluripotent stem cell aggregates in a serum-free medium containing a Wnt signaling pathway inhibitor
- Non-patent Document 3 and Patent Document 3 There are known methods for obtaining a multilayered retinal tissue by performing suspension culture in the presence of a basement membrane preparation and then performing suspension culture in a serum medium.
- Non-Patent Document 4 a method for inducing differentiation from pluripotent stem cells to hypothalamic tissue
- Non-Patent Documents 5 and 6 a method for inducing differentiation from pluripotent stem cells to neural progenitor cells
- primate pluripotent stem cells in particular, pluripotent stem cells, which are the starting materials for these production methods, have been undifferentiated and maintained in the presence of feeder cells and under the conditions for adding undifferentiated maintenance factors.
- Non-patent Document 7 Non-patent Document 8 and Non-patent document 9).
- a method for stably producing retinal cells or retinal tissue using pluripotent stem cells cultured in a feeder-free manner by this technique as a starting material has been desired.
- the problem to be solved by the present invention is to provide a method for producing retinal cells or retinal tissue from pluripotent stem cells prepared or cultured without maintenance of feeder cells in the absence of feeder cells.
- the present inventors have cultured pluripotent stem cells (particularly human iPS cells) in the absence of feeder cells, and obtained pluripotent stem cells.
- Suspension culture in a medium containing a sonic hedgehog signaling pathway agent to form cell aggregates that are round, smooth on the surface, dense inside the aggregates, and remain undifferentiated It was found that cell aggregates can be formed with high efficiency.
- the inventors have found that retinal cells and retinal tissues can be induced with high efficiency using the high-quality cell aggregates, and have completed the present invention. That is, the present invention relates to the following.
- a method for producing retinal cells or retinal tissue comprising the following steps (1) to (3); (1) a first step of culturing human pluripotent stem cells in a medium containing an undifferentiated maintenance factor in the absence of feeder cells; (2) a second step in which the pluripotent stem cells obtained in the first step are cultured in suspension in the presence of a sonic hedgehog signal transduction pathway agent to form cell aggregates; and (3) the second The third step of obtaining an aggregate containing retinal cells or retinal tissue by suspension culture of the aggregate obtained in the step in the presence of a substance acting on a BMP signal transduction pathway.
- the aggregate is cultured in a medium in which the concentration of the sonic hedgehog signaling pathway agonist is equal to or less than the concentration corresponding to the sonic hedgehog signaling activity of SAG700nM, [1] to [9 ]
- the manufacturing method in any one of. [11] The production method according to any one of [1] to [10], wherein the first step is performed by an adhesion culture method. [12] The production method according to any one of [1] to [11], wherein the pluripotent stem cell is an induced pluripotent stem cell. [13] The production method according to any one of [1] to [12], wherein a uniform aggregate is formed in the second step.
- the aggregate obtained in the third step is a retinal progenitor cell, neural retinal progenitor cell, photoreceptor precursor cell, photoreceptor cell, rod photoreceptor cell, cone photoreceptor cell, horizontal cell, amacrine cell, interneuron cell,
- a reagent for evaluating toxicity / drug efficacy of a test substance comprising retinal cells or retinal tissue produced by the method according to any one of [1] to [15].
- a test substance is brought into contact with a retinal cell or retinal tissue produced by the method according to any one of [1] to [15], and the effect of the substance on the cell or the tissue is assayed.
- a method for evaluating toxicity and drug efficacy of the substance [18]
- a therapeutic agent for diseases based on retinal tissue disorders comprising retinal cells or retinal tissue produced by the method according to any one of [1] to [15].
- retinal cells are retinal progenitor cells and / or retinal layer-specific nerve cells.
- retinal tissue disorders comprising transplanting an effective amount of retinal cells or retinal tissue produced by the method according to any one of [1] to [15] to a subject in need of transplantation Based disease treatment method.
- a pharmaceutical composition comprising a retinal cell or retinal tissue produced by the method according to any one of [1] to [15] as an active ingredient.
- high-quality cell aggregates, retinal cells and retinal tissues can be produced with high efficiency from pluripotent stem cells cultured in the absence of feeder cells.
- the culture conditions of Comparative Example 1 and Example 1, and bright field images (A-D) of the aggregates are shown.
- the culture conditions of Example 1 and immunohistochemically stained images (A-D) of aggregates relating to retinal tissue markers (Chx10, Rx) are shown.
- the culture conditions of Example 2 and the bright field image (A-D) of the aggregate are shown.
- the culture conditions of Example 2 (without BMP4 treatment) and the graph which quantified the grade of the form of the aggregate are shown.
- the culture conditions of Example 3 and immunohistochemically stained images (A-D) of aggregates relating to retinal tissue markers (Chx10, Rx) are shown.
- the immunostaining image by the retinal tissue marker of the retinal tissue manufactured on the culture conditions of Example 4 is shown.
- the immunostaining image by the retinal tissue marker of the retinal tissue manufactured on the culture conditions of Example 5 is shown.
- the bright field image (A-C) of the aggregate manufactured on the culture conditions of Example 6 and the immuno-staining image (D-F) by the retinal tissue marker (Chx10) are shown.
- the bright field image (A-D) of the aggregate manufactured on the culture conditions of Example 7 and the immuno-staining image (E) by the retinal tissue marker (Chx10) are shown.
- the bright field image (A, B) of the aggregate manufactured on the culture conditions of Example 8 and the immuno-staining image (C, D) by the retinal tissue marker (Chx10, Rx) are shown.
- stem cell means an undifferentiated cell having differentiation ability and proliferation ability (particularly self-renewal ability) while maintaining differentiation ability.
- the stem cells include subpopulations such as pluripotent stem cells, multipotent stem cells, unipotent stem cells, etc., depending on the differentiation ability.
- Pluripotent stem cells are capable of being cultured in vitro and capable of differentiating into all cell lineages belonging to three germ layers (ectodermal, mesoderm, endoderm) and / or tissues derived from extraembryonic tissues ( A stem cell having pluripotency.
- Multipotent stem cells mean stem cells that have the ability to differentiate into multiple types of tissues and cells, although not all types.
- a unipotent stem cell means a stem cell having the ability to differentiate into a specific tissue or cell.
- Pluripotent stem cells can be derived from fertilized eggs, cloned embryos, reproductive stem cells, stem cells in tissues, somatic cells, and the like.
- pluripotent stem cells include embryonic stem cells (ES cells: Embryonic cells), EG cells (Embryonic cells), induced pluripotent stem cells (iPS cells: induced cells).
- Muse cells Multi-lineage ⁇ differentiating Stress Enduring cells obtained from mesenchymal stem cells (mesenchymal stem cell; MSC) and GS cells prepared from germ cells (eg, testis) are also included in the pluripotent stem cells.
- Embryonic stem cells were established for the first time in 1981, and have been applied to the production of knockout mice since 1989.
- ES cells In 1998, human embryonic stem cells were established and are being used in regenerative medicine.
- ES cells can be produced by culturing the inner cell mass on feeder cells or in a medium containing LIF. Methods for producing ES cells are described, for example, in WO96 / 22362, WO02 / 101057, US5, 843, 780, US6, 200, 806, US6, 280, 718 and the like.
- Embryonic stem cells can be obtained from a predetermined institution or a commercial product can be purchased. For example, human embryonic stem cells KhES-1, KhES-2 and KhES-3 are available from the Institute of Regenerative Medicine, Kyoto University.
- EB5 cells, both mouse embryonic stem cells, are available from RIKEN, and the D3 strain is available from ATCC.
- ntES cells Nuclear transfer ES cells
- ntES cells one of ES cells, can be established from cloned embryos made by transplanting somatic cell nuclei into eggs from which cell lines have been removed.
- EG cells can be produced by culturing primordial germ cells in a medium containing mSCF, LIF and bFGF (Cell, 70: 841-847, 1992).
- the “artificial pluripotent stem cell” in the present invention is a cell in which pluripotency is induced by reprogramming somatic cells by a known method or the like.
- differentiated somatic cells such as fibroblasts and peripheral blood mononuclear cells are Oct3 / 4, Sox2, Klf4, Myc (c-Myc, N-Myc, L-Myc), Glis1, Nanog, Sall4, lin28.
- Preferred reprogramming factor combinations include (1) Oct3 / 4, Sox2, Klf4, and Myc (c-Myc or L-Myc), (2) Oct3 / 4, Sox2, Klf4, Lin28 and L-Myc (Stem Cells, 2013; 31: 458-466).
- Induced pluripotent stem cells were established in 2006 by mouse cells by Yamanaka et al. (Cell, 2006, 126 (4), pp.663-676). Artificial pluripotent stem cells were also established in human fibroblasts in 2007 and have pluripotency and self-renewal ability similar to embryonic stem cells (Cell, 2007, 131 (5), pp.861-872; Science , 2007, 318 (5858), pp.1917-1920; Nat. Biotechnol., 2008, 26 (1), pp.101-106).
- an artificial pluripotent stem cell can also be induced from a somatic cell by addition of a compound other than the method of producing by direct initialization by gene expression (Science, 2013, 341, pp. 651- 654). It is also possible to obtain established artificial pluripotent stem cells, such as 201B7 cells, 201B7-Ff cells, 253G1 cells, 253G4 cells, 1201C1 cells, 1205D1 cells, 1210B2 cells established at Kyoto University Alternatively, human induced pluripotent cell lines such as 1231A3 cells are available from Kyoto University and iPS Academia Japan. As established pluripotent stem cells, for example, Ff-I01 cells and Ff-I14 cells established at Kyoto University are available from Kyoto University.
- the somatic cells used for producing the induced pluripotent stem cells are not particularly limited, but tissue-derived fibroblasts, blood cells (eg, peripheral blood mononuclear cells and T cells), hepatocytes, pancreas Cell, intestinal epithelial cell, smooth muscle cell and the like.
- the means for expressing the gene is not particularly limited when it is initialized by the expression of several types of genes.
- the means include infection methods using viral vectors (for example, retrovirus vectors, lentivirus vectors, Sendai virus vectors, adenovirus vectors, adeno-associated virus vectors), plasmid vectors (for example, plasmid vectors, episomal vectors).
- viral vectors for example, retrovirus vectors, lentivirus vectors, Sendai virus vectors, adenovirus vectors, adeno-associated virus vectors
- plasmid vectors for example, plasmid vectors, episomal vectors.
- Gene transfer method used eg, calcium phosphate method, lipofection method, retronectin method, electroporation method
- gene transfer method using RNA vector eg, calcium phosphate method, lipofection method, electroporation method
- Artificial pluripotent stem cells can be produced in the presence of feeder cells or in the absence of feeder cells (feeder-free).
- induced pluripotent stem cells can be produced in the presence of an undifferentiated maintenance factor by a known method.
- the medium used for producing the induced pluripotent stem cells in the absence of feeder cells is not particularly limited. However, a known embryonic stem cell and / or artificial pluripotent stem cell maintenance medium, or feeder-free artificial medium can be used. A medium for establishing pluripotent stem cells can be used.
- Examples of the medium for establishing artificial pluripotent stem cells under feeder-free include feeder-free medium such as Essential 8 medium, TeSR medium, mTeSR medium, mTeSR-E8 medium, StemFit medium, and the like.
- feeder-free medium such as Essential 8 medium, TeSR medium, mTeSR medium, mTeSR-E8 medium, StemFit medium, and the like.
- artificial pluripotent stem cells can be produced by gene transfer of 4 factors of Oct3 / 4, Sox2, Klf4, and Myc into somatic cells in the absence of feeder cells using a Sendai virus vector .
- the pluripotent stem cell used in the present invention is preferably an ES cell or an induced pluripotent stem cell, more preferably an induced pluripotent stem cell.
- multipotent stem cells include hematopoietic stem cells, neural stem cells, retinal stem cells, mesenchymal stem cells and other tissue stem cells (also called tissue stem cells, tissue-specific stem cells or somatic stem cells).
- a genetically modified pluripotent stem cell can be prepared, for example, by using a homologous recombination technique.
- genes on the chromosome to be modified include cell marker genes, histocompatibility antigen genes, and disease-related genes based on nervous system cell disorders. Modifications of target genes on chromosomes include Manipulating Mouse Embryo, A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press (1994); Gene Targeting, A Practical Approach, IRL Press at Oxford University Press (1993); 8. Gene targeting, production of mutant mice using ES cells, methods described in Yodosha (1995) and the like can be used.
- genomic DNA containing a target gene to be modified (for example, cell marker gene, histocompatibility antigen gene, disease-related gene, etc.) is isolated, and the target gene is identified using the isolated genomic DNA.
- a target vector for homologous recombination is prepared. By introducing the prepared target vector into a stem cell and selecting a cell that has undergone homologous recombination between the target gene and the target vector, a stem cell in which the gene on the chromosome is modified can be prepared.
- Genomic DNA containing the target gene can be isolated by using a genomic DNA library screening system (Genome® Systems), Universal® Genome Walker® Kits (CLONTECH), or the like.
- genomic DNA a polynucleotide encoding the target protein can also be used.
- the polynucleotide can be obtained by amplifying the corresponding polynucleotide by PCR.
- the target vector can be either a replacement type or an insertion type.
- methods such as positive selection, promoter selection, negative selection, or poly A selection can be used.
- Examples of the method for selecting a target homologous recombinant from the selected cell lines include Southern hybridization method and PCR method for genomic DNA.
- the “mammal” in the present invention includes rodents, ungulates, felines, primates and the like. Rodents include mice, rats, hamsters, guinea pigs and the like. Hoofed animals include pigs, cows, goats, horses, sheep and the like. Cat eyes include dogs, cats and the like.
- the “primate” in the present invention refers to a mammal animal belonging to the primate, and examples of the primate include apes of primates such as lemurs, loris, and tsubai, and apes of primates such as monkeys, apes and humans.
- the pluripotent stem cells used in the present invention are human pluripotent stem cells, more preferably human induced pluripotent stem cells (iPS cells) or human embryonic stem cells (ES cells).
- iPS cells human induced pluripotent stem cells
- ES cells human embryonic stem cells
- the “floating culture” or “floating culture method” in the present invention refers to a method of culturing while maintaining a state in which cells or cell aggregates are suspended in a culture solution and performing the culturing.
- suspension culture is performed under conditions that do not allow cells or cell aggregates to adhere to culture equipment, etc.
- culture adheres
- the term “cell adheres” means that a strong cell-substratum junction is formed between the cell or the cell aggregate and the culture equipment.
- suspension culture refers to culture under conditions that do not allow a strong cell-substrate bond to be formed between cells or cell aggregates and culture equipment
- adhesion culture refers to cells or cells. Culturing under the condition that a strong cell-substrate bond is formed between the aggregate and the culture equipment.
- the cells adhere to each other.
- cell-matrix bonds are hardly formed between the culture equipment and the like, or even if formed, the contribution is small.
- the intrinsic cell-substrate bond is present inside the aggregate, but the cell-substrate bond is hardly formed between the culture apparatus and the like. Even if it is formed, its contribution is small.
- plane attachment means that the cells adhere to each other on the surface. More specifically, the cell-cell adhesion means that the proportion of the surface area of one cell that adheres to the surface of another cell is, for example, 1% or more, preferably 3% or more, more preferably 5%. That's it.
- the surface of the cell can be observed by staining with a reagent that stains the membrane (eg, DiI) or immunostaining of a cell adhesion factor (eg, E-cadherin or N-cadherin).
- the incubator used for the suspension culture is not particularly limited as long as it can perform “suspension culture”, and can be appropriately determined by those skilled in the art.
- Examples of such an incubator include a flask, a tissue culture flask, a culture dish (dish), a petri dish, a tissue culture dish, a multi-dish, a microplate, a microwell plate, a micropore, a multiplate, and a multiwell plate.
- These incubators are preferably non-cell-adhesive in order to enable suspension culture.
- the surface of the incubator is artificially treated for the purpose of improving cell adhesion (for example, extracellular matrix such as basement membrane preparation, laminin, entactin, collagen, gelatin, etc.) Etc., or those that have not been subjected to coating treatment with a polymer such as polylysine or polyornithine, or surface treatment such as positive charge treatment) can be used.
- cell adhesion for example, extracellular matrix such as basement membrane preparation, laminin, entactin, collagen, gelatin, etc.
- a polymer such as polylysine or polyornithine, or surface treatment such as positive charge treatment
- the surface of the incubator was artificially treated (for example, super hydrophilic treatment such as MPC polymer, low protein adsorption treatment, etc.) for the purpose of reducing the adhesion to cells. Things can be used. You may carry out rotation culture using a spinner flask, a roller bottle, etc. The culture surface of the incubator may be flat bottom or uneven.
- the incubator used for the adhesion culture is not particularly limited as long as it can “adherently culture”, and a person skilled in the art can appropriately incubate according to the culture scale, culture conditions, and culture period. Can be selected.
- Examples of such an incubator include a flask, a tissue culture flask, a culture dish (dish), a tissue culture dish, a multi-dish, a microplate, a microwell plate, a multiplate, a multiwell plate, a chamber slide, a petri dish, Examples include tubes, trays, culture bags, microcarriers, beads, stack plates, spinner flasks or roller bottles.
- These incubators are preferably cell adhesive in order to enable adhesion culture.
- Examples of the cell-adhesive incubator include an incubator in which the surface of the incubator is artificially treated for the purpose of improving adhesion with cells, specifically, a surface-treated incubator, or An incubator whose inside is coated with a coating agent can be mentioned.
- Examples of the coating agent include laminin [including laminin ⁇ 5 ⁇ 1 ⁇ 1 (hereinafter referred to as laminin 511), laminin ⁇ 1 ⁇ 1 ⁇ 1 (hereinafter referred to as laminin 111), and laminin fragments (such as laminin 511E8), entactin, collagen, gelatin, vitronectin (Vitronectin), Examples include an extracellular matrix such as Synthemax (Corning) and Matrigel, or a polymer such as polylysine and polyornithine.
- Examples of the surface-treated incubator include a surface-treated culture vessel such as a positive charge treatment.
- the medium used for cell culture can be prepared using a medium usually used for animal cell culture as a basal medium.
- the basal medium include BME medium, BGJb medium, CMRLCM1066 medium, Glasgow MEM (GMEM) medium, Improved MEM Zinc Option medium, IMDM medium, Medium 199 medium, Eagle MEM medium, ⁇ MEM medium, DMEM medium, F-12
- media that can be used for culturing animal cells such as media, DMEM / F12 media, IMDM / F12 media, ham media, RPMI ⁇ 1640 media, Fischer's media, or mixed media thereof.
- serum-free medium means a medium that does not contain unconditioned or unpurified serum.
- a medium containing purified blood-derived components or animal tissue-derived components is also included in the serum-free medium unless it contains unconditioned or unpurified serum.
- the serum-free medium may contain a serum replacement.
- serum substitutes include those containing albumin, transferrin, fatty acid, collagen precursor, trace elements, 2-mercaptoethanol or 3 ′ thiolglycerol, or equivalents thereof as appropriate.
- Such a serum substitute can be prepared, for example, by the method described in WO98 / 30679.
- a commercially available product may be used as a serum substitute.
- examples of such commercially available serum substitutes include Knockout TM Serum Replacement (manufactured by Life Technologies; currently ThermoFisher: hereinafter referred to as KSR), Chemically-defined Lipid concentrated (manufactured by Life Technologies), Glutamax TM ( Life Technologies), B27 (Life Technologies), N2 supplements (Life Technologies).
- the serum-free medium used in suspension culture contains fatty acids or lipids, amino acids (eg, non-essential amino acids), vitamins, growth factors, cytokines, antioxidants, 2-mercaptoethanol, pyruvate, buffers, inorganic salts, etc. You may contain.
- an appropriate amount for example, about 0.5% to about 30%, preferably about 1%) of a commercially available KSR (manufactured by Life Technologies) is used as such a serum-free medium.
- Serum-free medium supplemented with about 20% eg medium supplemented with 10% KSR, 1 x Chemically Defined Lipid Concentrate (CDLC) and 450 ⁇ M 1-monothioglycerol in a 1: 1 mixture of F-12 medium and IMDM medium
- CDLC Lipid Concentrate
- 450 ⁇ M 1-monothioglycerol in a 1: 1 mixture of F-12 medium and IMDM medium May be used.
- a medium disclosed in JP-T-2001-508302 can be cited as a KSR equivalent product.
- serum medium means a medium containing unadjusted or unpurified serum.
- the medium contains fatty acids or lipids, amino acids (eg, non-essential amino acids), vitamins, growth factors, cytokines, antioxidants, 2-mercaptoethanol, 1-monothioglycerol, pyruvic acid, buffers, inorganic salts, etc. May be.
- a serum medium can be used in the step of maintaining nerve tissue (eg, retinal tissue) produced according to the present invention (also referred to as mature culture).
- the culture in the present invention is preferably performed under xenofree conditions.
- “Zeno-free” means a condition in which a component derived from a biological species different from the biological species of the cell to be cultured is excluded.
- a medium containing substance X and “in the presence of substance X” means a medium added with exogenous substance X, a medium containing exogenous substance X, or exogenous substance X. Means in the presence of That is, when cells or tissues present in the medium express, secrete or produce the substance X endogenously, the endogenous substance X is distinguished from the foreign substance X, and the foreign substance It is understood that a medium not containing X does not fall under the category of “medium containing substance X” even if it contains endogenous substance X.
- medium containing a sonic hedgehog signaling pathway agent refers to a medium containing an exogenous sonic hedgehog signaling pathway agent or an exogenous sonic hedgehog signaling pathway agent. It is a culture medium containing.
- a feeder cell is a cell other than the stem cell that coexists when the stem cell is cultured.
- feeder cells used for undifferentiated maintenance culture of pluripotent stem cells include mouse fibroblasts (MEF, etc.), human fibroblasts, SNL cells and the like.
- a feeder cell subjected to growth inhibition treatment is preferable.
- the growth inhibitory treatment include treatment of a growth inhibitor (for example, mitomycin C), gamma ray irradiation or UV irradiation.
- Feeder cells used for undifferentiated maintenance culture of pluripotent stem cells can produce pluripotent stem cells by secreting humoral factors (preferably undifferentiated maintenance factors) and by preparing scaffolds for cell adhesion (extracellular matrix). Contributes to maintaining undifferentiation.
- humoral factors preferably undifferentiated maintenance factors
- extracellular matrix extracellular matrix
- the absence of feeder cells means culturing in the absence of feeder cells.
- the absence of feeder cells includes, for example, conditions in which no feeder cells are added, or conditions in which feeder cells are substantially not included (for example, the ratio of the number of feeder cells to the total number of cells is 3% or less). It is done.
- an “aggregate” of cells is a mass formed by aggregation of cells dispersed in a medium, and the cells are adhered to each other.
- Cell aggregates, embryoid bodies, spheres, and spheroids are also included in cell aggregates.
- the cells adhere to each other.
- cells form cell-cell junctions and / or cell adhesions, such as adhesion junctions. There is a case.
- an aggregate formed by cells dispersed at the start of suspension culture formed in the second step of the present invention [1], or the above-described present invention [1] An aggregate containing retinal cells induced to differentiate from pluripotent stem cells generated in the third step can be mentioned, and the “aggregate” is at the start of the second step of the present invention [1] (that is, suspension culture). Aggregates already formed at the start) are also included.
- the aggregate of cells generated in the second step includes an “embryoid body” (EB).
- uniform aggregate means that the size of each aggregate is constant when culturing a plurality of aggregates, and the size of the aggregate is evaluated by the length of the maximum diameter.
- the uniform aggregate means that the dispersion of the maximum diameter length is small. More specifically, 75% or more of the aggregate in the entire aggregate population is within the range of the average value ⁇ 100% of the maximum diameter in the aggregate population, preferably within the average value ⁇ 50%, more preferably Means within an average value of ⁇ 20%.
- to form a uniform aggregate means that “a certain number of dispersed cells are rapidly aggregated” when cells are aggregated to form cell aggregates and suspended in culture. Is to form a uniform cell aggregate.
- Dispersion means that cells and tissues are separated into small cell pieces (2 cells or more and 100 cells or less, preferably 50 cells or less) or single cells by dispersion treatment such as enzyme treatment or physical treatment.
- a certain number of dispersed cells refers to a collection of a certain number of cell pieces or single cells.
- Examples of the method for dispersing pluripotent stem cells include mechanical dispersion treatment, cell dispersion treatment, and cytoprotective agent addition treatment. You may perform combining these processes. Preferably, cell dispersion treatment is performed, followed by mechanical dispersion treatment.
- Examples of the cell dispersion used for the cell dispersion treatment include solutions containing any of enzymes such as trypsin, collagenase, hyaluronidase, elastase, pronase, DNase or papain, and a chelating agent such as ethylenediaminetetraacetic acid. Can do. Commercially available cell dispersions such as TrypLE Select (Life Technologies) or TrypLE Express (Life Technologies) can also be used.
- cytoprotective agent used for the cytoprotective agent treatment include FGF signal transduction pathway agonists, heparin, IGF signal transduction pathway agonists, serum, and serum substitutes.
- cytoprotective agent used for the cytoprotective agent treatment include FGF signal transduction pathway agonists, heparin, IGF signal transduction pathway agonists, serum, and serum substitutes.
- an inhibitor of Rho-associated coiled-coil kinase (ROCK) or an inhibitor of Myosin during dispersion May be added.
- ROCK inhibitors include Y-27632, Fasudil (HA1077), H-1152, and the like. Blebbistatin can be mentioned as an inhibitor of Myosin.
- Preferred cytoprotective agents include ROCK inhibitors.
- pluripotent stem cells can be dispersed by treating colonies of pluripotent stem cells with a cell dispersion (TrypLE ⁇ Select) in the presence of a ROCK inhibitor as a cytoprotective agent and further dispersing by pipetting. Can be mentioned.
- a cell is placed in a small space using a small well plate (for example, a plate having a well bottom area of about 0.1 to 2.0 cm 2 in terms of flat bottom) or a micropore.
- a small well plate for example, a plate having a well bottom area of about 0.1 to 2.0 cm 2 in terms of flat bottom
- a micropore A method of confinement and a method of aggregating cells by centrifuging for a short time using a small centrifuge tube can be mentioned.
- a 24-well plate (area is about 1.88 cm 2 in terms of flat bottom), 48-well plate (area is about 1.0 cm 2 in terms of flat bottom), 96-well plate (area is about 0.35 cm 2 in terms of flat bottom) , An inner diameter of about 6 to 8 mm), and a 384 well plate.
- a 96 well plate is used.
- the shape of the small plate of the well the shape of the bottom surface when the well is viewed from the top includes a polygon, a rectangle, an ellipse, and a perfect circle, preferably a perfect circle.
- the shape of the bottom surface when the well is viewed from the side may be a flat bottom structure or a structure in which the outer peripheral portion is high and the inner concave portion is low.
- Examples of the shape of the bottom surface include U bottom, V bottom, and M bottom, preferably U bottom or V bottom, and more preferably V bottom.
- a plate having a small well a plate having an unevenness or a depression on the bottom surface of a cell culture dish (for example, a 60 mm to 150 mm dish, a culture flask) may be used.
- the bottom surface of the plate having small wells is preferably a non-cell-adhesive bottom surface, preferably the cell-non-adhesive coated bottom surface.
- the formation of aggregates of pluripotent stem cells, or of cell populations containing pluripotent stem cells, and their uniformity are microscopically determined by aggregate size and number of cells, macroscopic morphology, and tissue staining analysis. It is possible to make a judgment based on the target form and its uniformity.
- the formation of epithelial-like structures in the aggregates, and the uniformity thereof are the macroscopic morphology of the aggregates, the microscopic morphology and homogeneity by tissue staining analysis, the expression of differentiation and undifferentiation markers, and the uniformity thereof. Determination based on sex, differentiation marker expression control and synchronization, and reproducibility between aggregates of differentiation efficiency.
- the “tissue” in the present invention is a structure of a cell population having a structure in which one type of cells having a uniform form or property, or a plurality of types of cells having different forms or properties are arranged in a three-dimensional pattern. Point.
- nerve tissue is composed of nervous system cells such as cerebral, midbrain, cerebellum, spinal cord, retina, peripheral nerve, forebrain, hindbrain, telencephalon, diencephalon, etc. Means an organization.
- the nerve tissue may form an epithelial structure (neuroepithelium) having a layer structure, and the abundance of the neuroepithelium in the cell aggregate can be evaluated by bright field observation using an optical microscope.
- neural cells refers to cells other than epidermal cells in the ectoderm-derived tissue. That is, it includes cells such as neural progenitor cells, neurons (neural cells), glia, neural stem cells, neuronal progenitor cells, and glial progenitor cells. Neural cells also include cells constituting the retinal tissue described below (retinal cells), retinal progenitor cells, retinal layer-specific neurons, neural retinal cells, and retinal pigment epithelial cells. Nervous cells can be identified using Nestin, TuJ1, PSA-NCAM, N-cadherin and the like as markers.
- Neurons Neurons (Neuron / Neuronal cell) are functional cells that form neural circuits and contribute to information transmission. Tumor markers such as TuJ1, Dcx, HuC / D, and / or Map2, NeuN, etc. The expression of the mature neuron marker can be identified using the index.
- glia examples include astrocytes, oligodendrocytes, and Muller glia.
- astrocyte markers include GFAP, oligodendrocyte markers O4, and Muller glia markers such as CRALBP.
- Neural stem cells are cells that have the ability to differentiate into nerve cells and glial cells (multipotency) and the ability to proliferate while maintaining pluripotency (sometimes referred to as self-replication ability).
- Nestin, Sox2, Musashi, Hes family, CD133, and the like can be mentioned as markers for neural stem cells, but these markers are general progenitor cell markers and are not considered neural stem cell-specific markers.
- the number of neural stem cells can be evaluated by a neurosphere assay, a clonal assay, or the like.
- Neuronal progenitor cells are cells that have proliferative ability, produce nerve cells, and do not produce glial cells. Examples of markers for neuronal progenitor cells include Tbr2, T ⁇ 1, and the like. Alternatively, juvenile neuron marker (TuJ1, Dcx, HuC / D) positive and proliferation marker (Ki67, pH3, MCM) positive cells can be identified as neuronal progenitor cells.
- a glial progenitor cell is a cell that has proliferative ability, produces glial cells, and does not produce nerve cells.
- Neural progenitor cells are aggregates of progenitor cells including neural stem cells, neuronal progenitor cells, and glial progenitor cells, and have proliferative ability and ability to produce neurons and glia.
- Neural progenitor cells can be identified using Nestin, GLAST, Sox2, Sox1, Musashi, Pax6, etc. as markers.
- neural cell marker-positive and proliferation marker Ki67, pH3, MCM
- retinal tissue refers to photoreceptor cells, photoreceptor precursor cells, rod photoreceptor cells, cone photoreceptor cells, interneuron cells, horizontal cells, bipolar cells, amacrine cells that constitute each retinal layer in the living retina.
- Retinal ganglion cells ganglion cells
- retinal pigment epithelial cells RPE
- ciliary marginal cells these progenitor cells, or cells such as retinal progenitor cells, one or at least multiple, layered and three-dimensional It means the tissue arranged in. Whether each cell constitutes which retinal layer can be confirmed by a known method, for example, the presence or absence of the expression of a cell marker or the degree thereof.
- the “retinal layer” in the present invention means each layer constituting the retina. Specifically, the retinal pigment epithelium layer, photoreceptor layer, outer boundary membrane, outer granular layer, outer reticulated layer, inner granular layer, inner granular layer, Mention may be made of the reticular layer, the ganglion cell layer, the nerve fiber layer and the inner limiting membrane.
- the “retinal progenitor cell” refers to a progenitor cell that can differentiate into any mature retinal cell such as a photoreceptor cell, a horizontal cell, a bipolar cell, an amacrine cell, a retinal ganglion cell, and a retinal pigment epithelial cell.
- neural retinal progenitor cell in the present invention refers to a progenitor cell that can also differentiate into any one or a plurality of mature retinal cells such as photoreceptor cells, horizontal cells, bipolar cells, amacrine cells, retinal ganglion cells, and the like. . In general, neural retinal progenitor cells do not differentiate into retinal pigment epithelial cells.
- Visual cell progenitor cells are photoreceptor cells, horizontal cells, bipolar cells, amacrine cell progenitor cells, retinal node cell progenitor cells, and retinal pigment epithelial progenitor cells are photoreceptor cells, horizontal cells, bipolar cells, amacrine cells, and retinal nerves, respectively.
- retinal layer-specific nerve cell means a cell constituting the retinal layer and specific to the retinal layer (neuron).
- retinal layer-specific neurons include bipolar cells, retinal ganglion cells, amacrine cells, horizontal cells, photoreceptor cells, retinal pigment epithelial cells, rod cells, and cone cells.
- the “retinal cells” in the present invention include the above-mentioned retinal progenitor cells and retinal layer-specific neurons.
- Rx also called Rax expressed in retinal progenitor cells
- PAX6 and Chx10 Nkx2.1 expressed in progenitor cells of hypothalamic neurons but not in retinal progenitor cells, expressed in hypothalamic neuroepithelium
- Sox1, which is not expressed in the retina Crx, Blimp1, which is expressed in progenitor cells of photoreceptors.
- Chx10, PKC ⁇ and L7 expressed in bipolar cells As markers for retinal layer-specific neurons, Chx10, PKC ⁇ and L7 expressed in bipolar cells, TuJ1 and Brn3 expressed in retinal ganglion cells, Calretinin expressed in amacrine cells, Calbindin expressed in horizontal cells, mature photoreceptor cells Rhodopsin and Recoverin expressed in rods, Nrl and Rhodopsin expressed in rod cells, Rxr-gamma and S-Opsin expressed in pyramidal cells, RPE65 and Mitf expressed in retinal pigment epithelial cells, expressed in the ciliary periphery Examples include Rdh10 and SSEA1.
- the production method of the present invention is a method for producing retinal cell or retinal tissue, comprising the following steps (1) to (3): (1) a first step of culturing human pluripotent stem cells in a medium containing an undifferentiated maintenance factor in the absence of feeder cells; (2) a second step in which the pluripotent stem cells obtained in the first step are cultured in suspension in the presence of a sonic hedgehog signal transduction pathway agent to form cell aggregates; and (3) the second A third step in which the aggregate obtained in the step is subjected to suspension culture in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing retinal cells or retinal tissue.
- step (1) human pluripotent stem cells are cultured in a medium containing an undifferentiated maintenance factor in the absence of feeder cells.
- the human pluripotent stem cell in step (1) is preferably a human induced pluripotent stem cell or a human embryonic stem cell (ES cell).
- the method for producing induced pluripotent stem cells is not particularly limited, and as described above, it can be produced by a method well known to those skilled in the art. It is desirable that the step of establishing pluripotent stem cells is also performed feeder-free.
- the production method of embryonic stem cells is not particularly limited, and can be produced by a method well known to those skilled in the art as described above.
- the embryonic stem cells (ES cells) production process is also feeder-free. It is desirable to do.
- the maintenance culture and expansion culture for obtaining pluripotent stem cells used in step (1) can be carried out by methods well known to those skilled in the art as described above.
- the maintenance culture / expansion culture of the pluripotent stem cells can be performed by adhesion culture or suspension culture, but is preferably performed by adhesion culture.
- the pluripotent stem cell maintenance culture / expansion culture step may be performed in the presence of a feeder or feeder-free, but preferably feeder-free.
- the absence of feeder cells means a condition that substantially does not contain feeder cells (for example, the ratio of the number of feeder cells to the total number of cells is 3% or less).
- step (1) is performed under conditions that do not include feeder cells.
- the medium used in step (1) is not particularly limited as long as it is a medium that enables undifferentiated maintenance culture of pluripotent stem cells under feeder-free conditions (feeder-free medium), but preferably undifferentiated
- an undifferentiated maintenance factor is included.
- a medium containing an undifferentiated maintenance factor and not containing a TGF ⁇ family signaling pathway inhibitor and a sonic hedgehog signaling pathway agonist is included.
- the undifferentiation maintenance factor is not particularly limited as long as it is a substance having an action of suppressing differentiation of pluripotent stem cells.
- undifferentiated maintenance factors widely used by those skilled in the art include FGF signaling pathway agents, TGF ⁇ family signaling Pathway action substances, insulin and the like.
- FGF signal transduction pathway agent include fibroblast growth factors (for example, bFGF, FGF4, and FGF8).
- TGF ⁇ family signal transduction pathway agonist include TGF ⁇ signal transduction pathway agonist and Nodal / Activin signal transduction pathway agonist.
- the medium in step (1) preferably contains bFGF as an undifferentiated maintenance factor.
- the undifferentiation maintenance factor used in the present invention is usually a mammalian undifferentiation maintenance factor.
- mammals include those described above. Since an undifferentiated maintenance factor can have cross-reactivity between mammalian species, any mammalian undifferentiated maintenance factor can be used as long as the undifferentiated state of the pluripotent stem cells to be cultured can be maintained. However, preferably, an undifferentiated maintenance factor of a mammal of the same species as the cell to be cultured is used.
- human undifferentiated maintenance factors eg, bFGF, FGF4, FGF8, EGF, Nodal, ActivinA, ActivinB, TGF ⁇ 1, TGF ⁇ 2, etc.
- human protein X means that protein X has the amino acid sequence of protein X that is naturally expressed in the human body.
- the undifferentiation maintenance factor used in the present invention is preferably isolated.
- isolated protein X means that an operation to remove a target component or a factor other than cells has been performed, and the state existing in nature has been removed. Therefore, “isolated protein X” does not include endogenous protein X produced from cells or tissues to be cultured and contained in the cells, tissues, or medium.
- the purity of “isolated protein X” (percentage of the weight of protein X in the total protein weight) is usually 70% or more, preferably 80% or more, more preferably 90% or more, still more preferably 99% or more, More preferably, it is 100%. Accordingly, in one aspect, the present invention includes providing an isolated undifferentiated maintenance factor.
- the process of adding the isolated undifferentiation maintenance factor exogenously (or exogenous) to the culture medium used for a process (1) is included.
- the undifferentiation maintenance factor may be added to the culture medium used for a process (1) previously.
- the undifferentiated maintenance factor concentration in the medium used in step (1) is a concentration capable of maintaining the undifferentiated state of the pluripotent stem cells to be cultured, and can be appropriately set by those skilled in the art.
- the concentration is usually about 4 ng to 500 ng / mL, preferably 10 ng to 200 ng / mL, more preferably about 30 ng to 150 ng / mL. .
- Essential 8 media Many synthetic media have been developed and marketed as feeder-free media, such as Essential 8 media.
- Essential 8 medium was added to DMEM / F12 medium as additives: L-ascorbic acid-2-phosphate magnesium (64 mg / l), sodium selenium (14 ⁇ g / 1), insulin (19.4 mg / l), NaHCO 3 (543 mg / l), transferrin (10.7 mg / l), bFGF (100 ng / mL), and TGF ⁇ family signal transduction pathway agonist (TGF ⁇ 1 (2 ng / mL) or Nodal (100 ng / mL)) (Nature Methods, 8, 424-429 (2011)).
- feeder-free media include, for example, Essential 8 (Life Technologies), S-medium (DS Pharma Biomedical), StemPro (Life Technologies), hESF9 (Proc Natl Acad Sci US A. 2008 Sep) 9; 105 (36): 13409-14), mTeSR1 (manufactured by STEMCELL Technologies), mTeSR2 (manufactured by STEMCELL Technologies), and TeSR-E8 (manufactured by STEMCELL Technologies).
- StemFit manufactured by Ajinomoto Co., Inc.
- the present invention can be easily carried out.
- the incubator used for the adhesion culture is not particularly limited as long as it can be “adherently cultured”, but a cell adhesive incubator is preferable.
- the cell-adhesive incubator include an incubator in which the surface of the incubator is artificially treated for the purpose of improving the adhesion to cells. Specifically, the inside described above is coated with a coating agent. Incubator.
- laminin 511 examples include laminin ⁇ 5 ⁇ 1 ⁇ 1 (hereinafter referred to as laminin 511), laminin ⁇ 1 ⁇ 1 ⁇ 1 (hereinafter referred to as laminin 111), and laminin fragments (such as laminin 511E8), entactin, collagen, gelatin, vitronectin (Vitronectin), Examples include an extracellular matrix such as Synthemax (Corning) and Matrigel, or a polymer such as polylysine and polyornithine.
- a culture vessel having a surface processed such as a positive charge treatment can also be used.
- laminin is mentioned, More preferably, laminin 511E-8 is mentioned.
- Laminin 511E-8 can be purchased commercially (eg, iMatrix-511, Nippi).
- the medium used in step (1) may be a serum medium or a serum-free medium, but is preferably a serum-free medium from the viewpoint of avoiding contamination with chemically undecided components.
- the medium used in the step (1) may be a medium in which the components are chemically determined from the viewpoint of avoiding contamination with chemically undecided components.
- the culture of pluripotent stem cells in step (1) may be performed under any conditions of suspension culture and adhesion culture, but is preferably performed by adhesion culture.
- the feeder-free medium may be used as the medium.
- an appropriate matrix may be used as the scaffold in order to provide a scaffold instead of feeder cells for pluripotent stem cells.
- the pluripotent stem cells are adhered and cultured in a cell container whose surface is coated with a matrix as a scaffold.
- the matrix that can be used as a scaffold includes laminin (Nat Biotechnol 28, 611-615 (2010)), laminin fragment (NatNCommun 3, 1236 (2012)), basement membrane preparation (Nat Biotechnol 19, 971-974 ( 2001)), gelatin, collagen, heparan sulfate proteoglycan, entactin, vitronectin and the like.
- laminin is a heterotrimeric molecule composed of ⁇ , ⁇ , and ⁇ chains, and is an extracellular matrix protein in which isoforms having different subunit chain compositions exist. Specifically, laminin has about 15 isoforms in a combination of 5 ⁇ chains, 4 ⁇ chains, and 3 ⁇ chain heterotrimers.
- the name of laminin is determined by combining the numbers of ⁇ chain ( ⁇ 1 to ⁇ 5), ⁇ chain ( ⁇ 1 to ⁇ 4) and ⁇ chain ( ⁇ 1 to ⁇ 4).
- laminin obtained by combining ⁇ 5 chain, ⁇ 1 chain, and ⁇ 1 chain is referred to as laminin 511.
- laminin 511 is preferably used (Nat Biotechnol 28, 611-615 (2010)).
- the laminin used in the present invention is usually a mammalian laminin.
- mammals include those described above.
- mammalian laminin of the same species as the cells to be cultured is preferably used.
- human laminin preferably human laminin 511 is used for culturing human pluripotent stem cells.
- the laminin fragment used in the present invention is not particularly limited as long as it has adhesion to pluripotent stem cells and enables maintenance culture of pluripotent stem cells under feeder-free conditions.
- the laminin E8 fragment was identified as a fragment having strong cell adhesion activity among fragments obtained by digesting laminin 511 with elastase (EMBO J., 3: 1463-1468, 1984, J. Cell Biol., 105: 589-598, 1987).
- the E8 fragment of laminin 511 is preferably used (Nat Commun 3, 1236 (2012), Scientific Reports 4, 3549 (2014)).
- the laminin E8 fragment used in the present invention does not need to be a laminin elastase digestion product, and may be a recombinant. From the viewpoint of avoiding contamination with unidentified components, a recombinant laminin fragment is preferably used in the present invention.
- the E8 fragment of laminin 511 is commercially available and can be purchased from, for example, Nippi Corporation.
- the laminin or laminin fragment used in the present invention is preferably isolated.
- the “basement membrane preparation” refers to epithelial cell-like cell morphology, differentiation, proliferation, movement, functional expression, etc., when desired cells having basement membrane forming ability are seeded and cultured thereon.
- the “basement membrane component” refers to a thin membrane-like extracellular matrix molecule that exists between an epithelial cell layer and a stromal cell layer in an animal tissue.
- a basement membrane preparation removes cells having a basement membrane-forming ability adhered to a support through a basement membrane from the support using a solution or an alkaline solution having lipid-dissolving ability of the cells.
- Basement membrane preparations include products that are commercially available as basement membrane preparations (for example, Matrigel TM (manufactured by Corning: hereinafter, sometimes referred to as Matrigel)), Geltrex TM (manufactured by Life Technologies), and basement membrane components. And those containing known extracellular matrix molecules (for example, laminin, type IV collagen, heparan sulfate proteoglycan, entactin and the like).
- Matrigel TM is a basement membrane preparation extracted from Engelbreth Holm Swarn (EHS) mouse sarcoma.
- the main components of Matrigel TM are type IV collagen, laminin, heparan sulfate proteoglycan and entactin.
- TGF ⁇ , FGF, tissue plasminogen activator and growth factors naturally produced by EHS tumors are included.
- Matrigel TM “growth factor reduced products” have lower growth factor concentrations than normal Matrigel TM , with standard concentrations of EGF ⁇ 0.5 ng / ml, NGF ⁇ 0.2 ng / ml, and PDGF ⁇ 5 pg / ml, IGF1 is 5 ng / ml, and TGF ⁇ is 1.7 ng / ml.
- isolated laminin or laminin fragments are preferably used in the present invention.
- the surface is coated with isolated laminin 511 or an E8 fragment of laminin 511 (more preferably, an E8 fragment of laminin 511).
- isolated laminin 511 or an E8 fragment of laminin 511 more preferably, an E8 fragment of laminin 511).
- human pluripotent stem cells are cultured for adhesion.
- the culture time of pluripotent stem cells in step (1) is not particularly limited as long as the effect of improving the quality of the aggregates formed in step (2) can be achieved, but is usually 0.5 to 144 hours, preferably Is 2 to 96 hours, more preferably 6 to 48 hours, still more preferably 12 to 48 hours, still more preferably 18 to 28 hours (eg, 24 hours). That is, the first step is started 0.5 to 144 hours (preferably 18 to 28 hours) before the start of the step (2), and the step (2) is continued after the completion of the step (1).
- the medium may be changed as appropriate.
- the medium may be replaced with a medium that does not contain a cytoprotective agent or a cell death inhibitor such as a ROCK inhibitor described later.
- the culture conditions such as culture temperature and CO 2 concentration in step (1) can be appropriately set.
- the culture temperature is, for example, about 30 ° C. to about 40 ° C., preferably about 37 ° C.
- the CO 2 concentration is, for example, about 1% to about 10%, preferably about 5%.
- human pluripotent stem cells are adherently cultured in a serum-free medium containing bFGF in the absence of feeder cells.
- the adhesion culture is preferably performed in a cell container whose surface is coated with laminin 511, an E8 fragment of laminin 511 or vitronectin.
- the adhesion culture is preferably performed using Essential 8, TeSR medium, mTeSR medium, mTeSR-E8 medium, or StemFit medium, more preferably Essential 8 or StemFit medium as a feeder-free medium.
- human pluripotent stem cells eg, human iPS cells
- a serum-free medium containing bFGF in the absence of feeder cells.
- human pluripotent stem cells may form aggregates of human pluripotent stem cells.
- step (2) of forming pluripotent stem cell aggregates by suspension culture of the pluripotent stem cells obtained in step (1) in the presence of a sonic hedgehog signal transduction pathway agonist is described. .
- the medium used in step (2) is not particularly limited as long as it is as described in the above definition section.
- the medium used in step (2) can be a serum-containing medium or a serum-free medium. From the viewpoint of avoiding contamination of chemically undecided components, a serum-free medium is preferably used in the present invention. For example, a serum-free medium to which no Wnt signaling pathway inhibitor is added can be used.
- a serum-free medium supplemented with an appropriate amount of a commercially available serum substitute such as KSR (for example, 10% KSR, 450 ⁇ M 1-mono in a 1: 1 mixture of IMDM and F-12) It is preferable to use a medium supplemented with thioglycerol and 1 x Chemically defined Lipid Concentrate, or a medium supplemented with 5% to 20% KSR, NEAA, pyruvate, 2-mercaptoethanol to GMEM.
- the amount of KSR added to the serum-free medium is usually about 1% to about 30%, preferably about 2% to about 20%.
- the pluripotent stem cells obtained in step (1) are dispersed.
- “Dispersed cells” obtained by the dispersion operation include, for example, a state in which 70% or more are single cells and 2-50 cell clusters are present in 30% or less.
- the dispersed cells preferably include a state in which 80% or more is a single cell and a mass of 2 to 50 cells is present in 20% or less.
- the dispersed cells include a state where adhesion between cells (for example, surface adhesion) is almost lost.
- dispersed cells include a state in which cell-cell junctions (eg, adhesive junctions) are almost lost.
- the dispersion operation of pluripotent stem cells obtained in step (1) may include the mechanical dispersion treatment, the cell dispersion treatment, and the cytoprotective agent addition treatment described above. You may perform combining these processes.
- the cell dispersion treatment is performed simultaneously with the cell protective agent addition treatment, and then the mechanical dispersion treatment is performed.
- cytoprotective agent used for the cytoprotective agent addition treatment examples include FGF signaling pathway acting substances, heparin, IGF signaling pathway acting substances, serum, and serum substitutes.
- inhibitors of Rho-associated coiled-coil kinase (ROCK) or Myosin Inhibitors may be added.
- an inhibitor of Rho-associated coiled-coil kinase (ROCK) or an inhibitor of Myosin is cultured in the second step. It may be added from the beginning.
- ROCK inhibitors examples include Y-27632, Fasudil (HA1077), H-1152, and the like. Blebbistatin can be mentioned as an inhibitor of Myosin.
- Examples of the cell dispersion used for the cell dispersion treatment include solutions containing any of enzymes such as trypsin, collagenase, hyaluronidase, elastase, pronase, DNase or papain, and a chelating agent such as ethylenediaminetetraacetic acid.
- enzymes such as trypsin, collagenase, hyaluronidase, elastase, pronase, DNase or papain
- a chelating agent such as ethylenediaminetetraacetic acid.
- Commercially available cell dispersions such as TrypLE Select (Life Technologies) or TrypLE Express (Life Technologies) can also be used.
- Dispersed cells are suspended in the above medium.
- a suspension of dispersed pluripotent stem cells is seeded in the incubator, and the dispersed pluripotent stem cells are cultured on the incubator under non-adhesive conditions. Cells are aggregated to form aggregates.
- an aggregate of a plurality of cells may be simultaneously formed in one incubator, This causes variations in the size of each aggregate. Therefore, for example, when a certain number of dispersed pluripotent stem cells are placed in each well of a multiwell plate (U bottom, V bottom) such as a 96-well microplate, Aggregation forms one aggregate in each well. By collecting the aggregates from a plurality of wells, a uniform aggregate population can be obtained.
- the concentration of the pluripotent stem cells in the step (2) can be appropriately set so as to form a cell aggregate more uniformly and efficiently.
- human cells eg, human iPS cells obtained from step (1)
- a solution prepared to have about 8 ⁇ 10 3 to about 1.2 ⁇ 10 4 cells is added to the well, and the plate is allowed to stand to form an aggregate.
- the culture conditions such as culture temperature and CO 2 concentration in step (2) can be appropriately set.
- the culture temperature is, for example, about 30 ° C. to about 40 ° C., preferably about 37 ° C.
- the CO 2 concentration is for example about 1% to about 10%, preferably about 5%.
- step (2) when the medium is exchanged, for example, an operation of adding a new medium without discarding the original medium (medium addition operation), about half of the original medium (30 to 90 of the volume of the original medium) % (For example, about 40-60%) discarding and adding about half of the new medium (30-90% of the original medium volume, for example, about 40-60%) (half-volume medium replacement operation)
- Examples include an operation (total medium replacement operation) of discarding the whole amount (90% or more of the volume of the original medium) and adding a new medium (about 90% or more of the volume of the original medium).
- a specific component for example, a differentiation-inducing factor
- discard about half of the original medium and concentrate the specific component at a concentration higher than the final concentration specifically Specifically, an operation of adding about half amount of a new medium containing 1.5 to 3.0 times the final concentration (for example, about twice the final concentration) (half amount medium exchange operation) may be performed.
- the medium exchange operation is performed several times a day, preferably several times within one hour (for example, 2 to 3 times). Also good.
- the cells or aggregates may be transferred to another culture vessel.
- the tool used for the medium exchange operation is not particularly limited, and examples thereof include a pipetter, a micropipette (Pipetteman), a multichannel micropipette (Multichannel Pipetman), and a continuous dispenser.
- a pipetter a pipetter
- a micropipette Peripheral Component Interconnect
- a multichannel micropipette Multichannel Pipetman
- a continuous dispenser e.g., a 96-well plate is used as a culture container
- a multichannel micropipette multichannel pipetteman
- the suspension culture time required to form cell aggregates can be determined as appropriate depending on the pluripotent stem cells used so that the cells are aggregated uniformly, but in order to form uniform cell aggregates, Is preferably as short as possible.
- the process until the dispersed cells reach the formation of cell aggregates is divided into a process in which cells aggregate and a process in which the aggregated cells form cell aggregates. From the time of seeding the dispersed cells (ie, at the start of suspension culture) to the time of cell assembly, for example, in the case of human pluripotent stem cells (eg, human iPS cells), preferably within about 24 hours, more Preferably, aggregated cells are formed within about 12 hours.
- preferably about 72 Aggregates are formed within a time period, more preferably within about 48 hours.
- the time until the formation of the aggregates can be appropriately adjusted by adjusting tools for aggregating cells, centrifugation conditions, and the like.
- the formation of the aggregates of cells and the uniformity thereof were determined by the size and number of the aggregates, the macroscopic morphology, the microscopic morphology by tissue staining analysis and the uniformity thereof, the expression of differentiation and undifferentiation markers and the It is possible to make a judgment based on homogeneity, differentiation marker expression control and synchronization, reproducibility between aggregates of differentiation efficiency, and the like.
- the suspension culture time in step (2) is usually about 12 hours to 6 days, preferably about 12 hours to 48 hours.
- the medium used in step (2) contains a sonic hedgehog signal transduction pathway agent.
- pluripotent stem cells are maintained and cultured under feeder-free conditions, and in the second step, the pluripotent stem cells obtained in the first step are cultured in a medium containing a sonic hedgehog signal transduction pathway agent (
- the aggregate quality is improved by subjecting it to suspension culture in a serum-free medium) to improve the quality of the aggregate, which is round, smooth, unshaped, and densely packed inside.
- aggregates of cells that maintain undifferentiation can be formed with high efficiency.
- Sonic hedgehog (hereinafter sometimes referred to as Shh) signal transduction pathway agonist is a substance that can enhance signal transduction mediated by Shh.
- substances that act on the Shh signaling pathway include proteins belonging to the Hedgehog family (eg, Shh and Ihh), Shh receptors, Shh receptor agonists, Purmorphamine (PMA; 9-cyclohexyl-N- [4- (4-morpholinyl) ) Phenyl] -2- (1-naphthalenyloxy) -9H-purin-6-amine), or SAG (SmoothenedtheAgonist; N-methyl-N ′-(3-pyridinylbenzyl) -N ′-( 3-chlorobenzo [b] thiophene-2-carbonyl) -1,4-diaminocyclohexane) and the like.
- PMA 9-cyclohexyl-N- [4- (4-morpholinyl)
- the Shh signal transduction pathway agent examples include SAG, Purmorphamine (PMA) and Shh protein (Genbank accession numbers: NM_000193 and NP_000184), more preferably SAG.
- concentration of the Shh signal transduction pathway agent in the medium can be appropriately set within a range where the above-described effects can be achieved.
- SAG is usually used at a concentration of 1 to 2000 nM, preferably 10 nM to 1000 nM, preferably 10 nM to 700 nM, 30 nM to 700 nM, 50 nM to 700 nM, more preferably 100 nM to 600 nM, and even more preferably 100 nM to 500 nM.
- the concentration is usually about 10 to 1000 ng / ml, preferably about 10 to 300 ng / ml. It corresponds to.
- the concentration of the sonic hedgehog signal transduction pathway agent in the medium may be varied during the step (2).
- the sonic hedgehog signal transduction pathway agonist is within the above range, and the concentration is gradually or stepwise reduced at a rate of 40-60% per 2-4 days. You may let them.
- the timing of adding the sonic hedgehog signal transduction pathway agent to the medium is not particularly limited as long as the above effect can be achieved, but the earlier the higher the effect.
- a sonic hedgehog signaling pathway agent is added to the medium usually within 3 days, preferably within 2 days, more preferably within 1 day from the start of step (2), and even more preferably at the start of step (2).
- the human pluripotent stem cell (eg, human iPS cell) obtained in step (1) is treated with a sonic hedgehog signaling pathway agent (eg, SAG, Shh protein, It is subjected to suspension culture in a serum-free medium containing PMA) to form an aggregate.
- a sonic hedgehog signaling pathway agent eg, SAG, Shh protein, It is subjected to suspension culture in a serum-free medium containing PMA
- the sonic hedgehog signal transduction pathway agent is preferably contained in the medium from the start of suspension culture.
- a ROCK inhibitor eg, Y-27632
- the culture time is 12 hours to 6 days, preferably 12 hours to 48 hours.
- the aggregate formed is preferably a uniform aggregate.
- the human pluripotent stem cells obtained in step (1) are collected and dispersed into a single cell or a state close thereto, and the sonic hedgehog signaling pathway Suspend in a serum-free medium containing an active substance (eg, SAG, Shh protein, PMA) and subject to suspension culture.
- the serum-free medium may contain a ROCK inhibitor (eg, Y-27632).
- a suspension of human pluripotent stem cells eg, iPS cells
- the dispersed pluripotent stem cells are cultured under non-adherent conditions in the incubator.
- a plurality of pluripotent stem cells are aggregated to form an aggregate.
- the culture time is 12 hours to 6 days, preferably 12 hours to 48 hours.
- the aggregate formed is preferably a uniform aggregate.
- step (2) pluripotent stem cells obtained in step (1) or aggregates of cells derived therefrom are formed.
- the present invention also provides a method for producing such an aggregate.
- the aggregate obtained in step (2) has a higher quality than in the case where it is not treated with a sonic hedgehog signal transduction pathway agent in step (2). Specifically, it is possible to obtain a population of aggregates that are round, have a smooth surface, are dense inside, and have a high proportion of aggregates that are not deformed.
- the ratio of aggregates that have not collapsed and / or the ratio of aggregates that have not become cysts Is, for example, 30% or more, preferably 50% or more.
- the aggregate obtained in step (2) has the ability to differentiate into various differentiated cells and tissues.
- the aggregate obtained in step (2) has the ability to differentiate into nervous system cells (eg, retinal cells) and neural tissues (eg, retinal tissues).
- stem cells preferably, obtained in step (1) having the ability to differentiate into at least neural cells and neural tissues (preferably retinal tissue, retinal progenitor cells, or retinal layer-specific neural cells).
- step (2) at least a nervous system cell or a nerve tissue (preferably an Oct3 / 4-positive stem cell having the ability to differentiate into a retinal tissue, a retinal progenitor cell, or a retinal layer-specific nerve cell), Obtaining an aggregate containing at least stem cells (for example, Oct3 / 4-positive stem cells) capable of differentiating into nervous system cells and neural tissues (preferably retinal tissue, retinal progenitor cells, or retinal layer-specific neural cells). it can.
- Aggregates obtained in step (2) can be obtained by culturing the aggregates under appropriate differentiation conditions, so that various differentiated cells and differentiated tissues (eg, neural cells such as retinal cells, nerves such as retinal tissues, etc.) Organization) can be guided with high efficiency.
- various differentiated cells and differentiated tissues eg, neural cells such as retinal cells, nerves such as retinal tissues, etc.
- the aggregate obtained in step (2) includes a pluripotent stem cell obtained in step (1) and a nervous system cell or nerve tissue (preferably a retinal cell or retinal tissue).
- a nervous system cell or nerve tissue preferably a retinal cell or retinal tissue.
- Cells corresponding to intermediate stage cells are included.
- the cells are pluripotent property marker Oct3 / 4, ectoderm marker (Sox1, Sox2, N-cadherin, TP63), neuroectodermal marker (Sox1, Sox2, Nestin, N-cadherin, Otx2), the aforementioned nervous system Expresses one of the cell markers.
- the aggregate obtained in step (2) includes pluripotency property marker Oct3 / 4, ectoderm marker (Sox1, Sox2, N-cadherin, TP63), neuroectodermal marker (Sox1, Sox2) Nestin, N-cadherin, Otx2), a mixture of cells expressing any of the aforementioned neural cell markers. That is, the aggregate obtained in the step (2) contains at least stem cells having the ability to differentiate into neural cells or neural tissues (preferably retinal cells or retinal tissues), and / or neural cells or neural tissues (preferably Includes precursor cells of retinal cells or retinal tissue.
- the progenitor cells are characterized by having the ability to express the aforementioned neural cell marker (preferably, a retinal cell marker) when cultured under known appropriate culture conditions.
- the aggregate obtained in step (2) is an Oct3 / 4-positive stem cell having the ability to differentiate into at least a nervous system cell or nerve tissue (preferably a retinal cell or retinal tissue), and / or Alternatively, it includes a precursor cell of a nervous system cell or nerve tissue (preferably, a retinal cell or retinal tissue). A part of the cells contained in the aggregate obtained in the step (2) may express the above-mentioned neural tissue marker.
- the aggregate obtained in the step (2) may contain 50% or more of, for example, 70% or more of Oct3 / 4 positive in all cells.
- step (2) when the medium is exchanged, for example, an operation of adding a new medium without discarding the original medium (medium addition operation), about half of the original medium (30 to 90 of the volume of the original medium) % (For example, about 40-60%) discarding and adding about half of the new medium (about 30-90% of the original medium volume, for example, about 40-60%) (half-volume medium replacement operation), original medium
- the total amount (90% or more of the volume of the original medium) is discarded and a new medium is added (about 90% or more of the volume of the original medium) (total medium replacement operation).
- a specific component for example, a differentiation-inducing factor
- discard about half of the original medium and concentrate the specific component at a concentration higher than the final concentration specifically, for example, an operation of adding about half amount of a new medium containing 1.5 to 3.0 times the final concentration (for example, about twice the final concentration) may be performed (half amount medium replacement operation).
- the medium exchange operation is performed several times a day, preferably several times within one hour (for example, 2 to 3 times). Also good.
- the cells or aggregates may be transferred to another culture vessel.
- the tool used for the medium exchange operation is not particularly limited, and examples thereof include a pipetter, a micropipette (Pipetteman), a multichannel micropipette (Multichannel Pipetman), and a continuous dispenser.
- a pipetter a pipetter
- a micropipette Peripheral Component Interconnect
- a multichannel micropipette Multichannel Pipetman
- a continuous dispenser e.g., a 96-well plate is used as a culture container
- a multichannel micropipette multichannel pipetteman
- Step (3) for inducing an aggregate containing retinal cells or retinal tissue from the aggregate obtained in step (2) will be described.
- the aggregate obtained in step (2) can be suspended in the presence or absence (preferably in the presence) of an appropriate differentiation-inducing factor to obtain an aggregate containing retinal cells or retinal tissue.
- the aggregate obtained in step (2) is applied to a nervous system cell containing a retinal cell in a serum medium or a serum-free medium.
- a method for inducing spontaneous differentiation is mentioned.
- the serum medium or serum-free medium include serum medium or serum-free medium that does not contain an undifferentiated maintenance factor.
- Retinal cells generated in the process of differentiation into nervous system cells may be selected and isolated. FACS or MACS may be used for sorting and isolation.
- pluripotent stem cells that can easily produce retinas may be used as starting materials for step (1).
- the aggregate obtained in the step (2) is suspended in the presence of an appropriate differentiation-inducing factor to obtain an aggregate containing retinal cells or retinal tissue.
- the differentiation-inducing factor used here is not particularly limited as long as it has an activity of inducing differentiation of retinal cells or retinal tissues.
- a BMP signaling pathway agent for example, a BMP signaling pathway agent, a basement membrane preparation, Wnt signaling Pathway inhibitor, TGF ⁇ family signal transduction pathway inhibitor, and the like.
- the response to the differentiation-inducing factor varies depending on the cell type and differentiation state (competence or potential), and even in the differentiation-inducing process, the effect of the differentiation-inducing factor may vary depending on the concentration and addition timing of the differentiation-inducing factor.
- the optimum concentration of a differentiation-inducing factor having the same effect varies depending on the animal species.
- human cells have higher optimum concentrations in mouse cells and human cells. (Especially in the ectoderm and endoderm). Many methods for inducing differentiation from pluripotent stem cells to specific cells or tissues have been reported, and a differentiation-inducing factor or differentiation-inducing method suitable for the target cell or tissue can be selected.
- a BMP signal transduction pathway agent is used as a differentiation inducing factor. That is, the aggregate obtained in the step (2) is suspended in the presence of a substance acting on the BMP signal transduction pathway to obtain an aggregate containing retinal cells or retinal tissue.
- a BMP signal transduction pathway agent is a substance that can enhance a signal transduction pathway mediated by BMP.
- BMP signal transduction pathway agonists include BMP proteins such as BMP2, BMP4 and BMP7, GDF proteins such as GDF7, anti-BMP receptor antibodies, and BMP partial peptides.
- BMP2 protein, BMP4 protein and BMP7 protein are available from, for example, R & D® Systems, and GDF7 protein is available from, for example, Wako Pure Chemical Industries.
- the BMP signaling pathway agent is preferably BMP4.
- the differentiation-inducing factor (eg, BMP4) used in the present invention is usually a mammalian differentiation-inducing factor.
- mammals include those described above. Since differentiation-inducing factors may have cross-reactivity between mammalian species, any mammalian undifferentiation maintenance factor may be used as long as differentiation induction of pluripotent stem cells to be cultured is possible.
- a mammalian differentiation inducer of the same species as the cell to be cultured is used.
- a human differentiation-inducing factor eg, BMP4 is preferably used.
- the differentiation-inducing factor (eg, BMP4) used in the present invention is preferably isolated. Accordingly, in one embodiment, the present invention includes the step of providing an isolated differentiation inducer (eg, BMP4). Moreover, in one aspect
- the medium used in step (3) is a serum-free medium or a serum medium (preferably a serum-free medium) supplemented with a differentiation-inducing factor (eg, BMP4).
- a serum-free medium preferably a serum-free medium
- a differentiation-inducing factor eg, BMP4
- Such a medium may or may not contain a basement membrane preparation.
- the basement membrane preparation those described above can be used.
- the concentration in the case of adding the basement membrane preparation is, for example, 0.1 to 10%, more preferably 0.5% to 2% by volume when using Matrigel. From the viewpoint of avoiding contamination with chemically unidentified substances, no basement membrane preparation is added.
- the serum-free medium or serum medium used for such a medium is not particularly limited as long as it is as described above.
- a serum-free medium supplemented with an appropriate amount of a commercially available serum substitute such as KSR (for example, 10% KSR, 450 ⁇ M 1 ⁇ in a 1: 1 mixture of IMDM and F-12)
- KSR for example, 10% KSR, 450 ⁇ M 1 ⁇ in a 1: 1 mixture of IMDM and F-12
- a medium to which monothioglycerol and 1 xChemically Defined Lipid Concentrate are added, or a medium to which 5% to 20% KSR, NEAA, pyruvate, 2-mercaptoethanol is added to GMEM).
- the amount of KSR added to the serum-free medium is usually about 1% to about 20%, preferably about 2% to about 20%.
- the medium (preferably serum-free medium) used in step (3) can be used as it is, or a new medium (preferably serum-free medium). Can also be replaced.
- the serum-free medium containing no differentiation-inducing factor (eg, BMP4) used in step (2) is used as it is in step (3), the differentiation-inducing factor (eg, BMP4) may be added to the medium.
- the concentration of the Shh signaling pathway agent added to the medium in step (2) is relatively low (eg, 700 nM or less for SAG, In the case of a concentration equal to or lower than that of SAG, the medium does not need to be replaced, and a differentiation-inducing factor (eg, BMP4) may be added to the medium used in step (2).
- a differentiation-inducing factor eg, BMP4
- the concentration of the substance acting on the Shh signal transduction pathway is relatively high (for example, more than 700 nM for SAG or 1000 nM or more, and for other substances acting on the Shh signal transduction pathway, Shh signal transduction promotion is equivalent to SAG at the above concentration.
- the concentration of the Shh signaling pathway agent in the medium used in step (3) is 700 nM or less, preferably 300 nM or less, more preferably 10 nM or less in terms of the Shh signaling promoting activity of SAG. More preferably, it is not more than 0.1 nM, and more preferably does not contain an Shh signaling pathway agent.
- the medium that does not contain an Shh signaling pathway agent is a medium that does not substantially contain an Shh signaling pathway agent, for example, a concentration that adversely affects selective differentiation into retinal cells and retinal tissues. Medium containing no Shh signaling pathway agonist is also included.
- “Shh signaling pathway agonists are not added” medium has a detrimental effect on selective differentiation into retinal cells and retinal tissues, eg, media that are substantially free of Shh signaling pathway agonists. Also included is a medium that does not contain a concentration of the Shh signaling pathway agent that provides a concentration.
- the concentration of the differentiation-inducing factor (eg, BMP signal transduction pathway agent) in the medium may be any concentration that can induce differentiation of pluripotent stem cells or cell aggregates derived therefrom into retinal cells.
- the concentration is about 0.01 nM to about 1 ⁇ M, preferably about 0.1 nM to about 100 nM, more preferably about 1 nM to about 10 nM, and more preferably about 1.5 nM (55 ng / mL).
- Add to medium. When using a BMP signal transduction pathway agent other than BMP4, it is desirable to use it at a concentration that exhibits BMP signal transduction promoting activity equivalent to the concentration of BMP4.
- the concentration of differentiation-inducing factor (eg, BMP4) in the medium may be varied during the step (3).
- the differentiation-inducing factor (eg, BMP4) is within the above range, and the concentration is decreased gradually or stepwise at a rate of 40-60% reduction every 2-4 days. May be.
- Differentiation-inducing factors need only be added after about 24 hours from the start of suspension culture in step (2), and are added to the medium within a few days (eg, within 15 days) after the start of suspension culture. May be.
- the differentiation-inducing factor eg, BMP4
- the differentiation-inducing factor is used from the first day to the 15th day after the start of suspension culture, more preferably from the first day to the ninth day, and further preferably from the third day to the eighth day. It is added to the medium during the day, even more preferably between the 3rd and 6th day.
- a differentiation-inducing factor eg, BMP4
- the medium may be exchanged using a serum-free medium or serum medium not containing (eg, BMP4).
- a differentiation-inducing factor eg, a medium
- BMP4 Concentration is reduced gradually or stepwise at a rate of 40-60% reduction every 2-4 days. Thereby, the concentration of a differentiation-inducing factor (eg, BMP4) can be serially diluted, and the effect / action time of the differentiation-inducing factor can be limited.
- the final concentration of BMP4 is adjusted to about 1 to 10 nM, and cultured in the presence of BMP4 for 1 to 12 days, preferably 2 to 9 days, more preferably 2 to 5 days. be able to.
- a part or all of the medium can be exchanged with a medium containing BMP4 about once or twice.
- concentration of BMP4 can also be reduced in steps.
- Cells in which differentiation induction into retinal cells has been initiated can be confirmed, for example, by detecting the expression of a retinal progenitor cell marker gene (eg, Rx gene (also known as Rax), Pax6 gene, Chx10 gene) in the cell. it can.
- a retinal progenitor cell marker gene eg, Rx gene (also known as Rax), Pax6 gene, Chx10 gene
- Differentiation-inducing factors eg, the concentration required for inducing differentiation into retinal cells
- a fluorescent reporter protein gene such as GFP is knocked into the Rx locus
- the time when the induction of differentiation into retinal cells was started can be confirmed by culturing in suspension in the presence of BMP4) and detecting fluorescence emitted from the expressed fluorescent reporter protein.
- the aggregate formed in the step (2) is used until a cell expressing a retinal progenitor cell marker gene (eg, Rx gene, Pax6 gene, Chx10 gene) begins to appear.
- a step of floating culture in a serum-free medium or serum medium containing a differentiation-inducing factor (eg, BMP4) at a concentration necessary for inducing differentiation into retinal cells to obtain an aggregate containing retinal progenitor cells. can do.
- step (3) when the medium replacement operation is performed, for example, the above-described medium addition operation, half-volume medium replacement operation, and full-volume medium replacement operation may be mentioned.
- a specific component for example, a differentiation-inducing factor
- discard about half of the original medium and concentrate the specific component at a concentration higher than the final concentration specifically, for example, an operation of adding about a half amount of a new medium containing 1.5 to 3.0 times the final concentration (for example, a concentration about twice the final concentration) may be performed.
- the medium exchange operation is performed several times a day, preferably several times within one hour (for example, 2 to 3 times). Also good.
- the cells or aggregates may be transferred to another culture vessel.
- the tool used for the medium exchange operation is not particularly limited, and examples thereof include a pipetter, a micropipette (Pipetteman), a multichannel micropipette (Multichannel Pipetman), and a continuous dispenser.
- a pipetter a pipetter
- a micropipette Peripheral Component Interconnect
- a multichannel micropipette Multichannel Pipetman
- a continuous dispenser e.g., a 96-well plate is used as a culture container
- a multichannel micropipette multichannel pipetteman
- the culture conditions such as culture temperature and CO 2 concentration in step (3) can be appropriately set.
- the culture temperature is, for example, about 30 ° C. to about 40 ° C., preferably about 37 ° C.
- the CO 2 concentration is, for example, about 1% to about 10%, preferably about 5%.
- the present invention provides a method for producing an aggregate containing such retinal progenitor cells.
- the fact that aggregates containing retinal progenitor cells have been obtained can be confirmed, for example, by detecting that cells expressing Rx, PAX6 or Chx10, which are markers of retinal progenitor cells, are contained in the aggregates. it can.
- the aggregate formed in the step (2) is used for the BMP at a concentration necessary for inducing differentiation into retinal cells until cells expressing the Rx gene begin to appear.
- Examples thereof include a step of suspension culture in a serum-free medium or serum medium containing a signal transduction pathway agent (eg, BMP4) to obtain an aggregate containing retinal progenitor cells.
- a signal transduction pathway agent eg, BMP4
- the step (3) Culture is performed.
- the obtained aggregate containing retinal progenitor cells may be used as it is as a reagent for evaluating toxicity and drug efficacy.
- High-purity retinal progenitor cells can be obtained by dispersing aggregates containing retinal progenitor cells (for example, trypsin / EDTA treatment or papain treatment) and sorting the resulting cells using FACS or MACS It is.
- the retinal tissue-containing retinal tissue can be further differentiated by further culturing the aggregate containing the retinal tissue in a serum-free medium or serum medium to further differentiate the retinal cells (eg, retinal progenitor cells) contained in the retinal tissue.
- the retinal cells eg, retinal progenitor cells
- the serum-free medium or serum medium used for such a medium is not particularly limited as long as it is as described above.
- the culture time for inducing retinal tissue from retinal progenitor cells varies depending on the target retinal layer-specific neurons, but is, for example, about 7 days to about 200 days.
- the retinal tissue exists so as to cover the surface of the aggregate. After the suspension culture is completed, the aggregate is fixed using a fixative such as a paraformaldehyde solution, a frozen section is prepared, and then it is confirmed by immunostaining that a retinal tissue having a layer structure is formed. . Since retinal tissue has different cells (visual cells, horizontal cells, bipolar cells, amacrine cells, retinal ganglion cells) that make up each layer, immunity can be achieved using antibodies against the above-mentioned markers expressed in these cells. It can be confirmed that a layer structure is formed by a staining method. In one embodiment, the retinal tissue is an Rx or Chx10 positive neuroepithelial structure.
- the aggregate obtained in step (3) includes retinal tissue and substantially does not include head non-neural ectoderm.
- the aggregate containing retinal tissue and substantially free of head non-neuronal ectoderm for example, in the immunostained image of the aggregate frozen section described above, an Rx-positive tissue is observed, and an Rx-negative tissue is present outside thereof. Not observed.
- the aggregate formed in the step (2) is used for the BMP at a concentration necessary for inducing differentiation into retinal cells until cells expressing the Rx gene begin to appear.
- Suspension culture in a serum-free medium or serum medium containing a signal transduction pathway agent to obtain an aggregate containing retinal progenitor cells, and the serum containing retinal progenitor cells is continuously serum-free until retinal tissue is formed A step of suspension culture in a medium or a serum medium to obtain an aggregate containing retinal tissue can be mentioned.
- medium exchange with serum-free medium or serum medium that does not contain a BMP signaling pathway agent is performed.
- the concentration of BMP signaling pathway agent contained in the medium to induce retinal progenitor cells may be decreased gradually or stepwise at a rate of 40-60% reduction every 2-4 days .
- retinal progenitor cells are included until 20% or more (preferably 30%, 40%, 50%, 60% or more) of the cells contained in the aggregate are in a state of expressing Chx10. Aggregate suspension culture is performed.
- the aggregate obtained in the step (2) or the aggregate obtained by suspension culture of the aggregate obtained in the step (2) by the above method is used for adhesion culture.
- the adhesion aggregate is adhered in a serum-free medium or serum medium containing a differentiation-inducing factor (eg, BMP4) at a concentration necessary for inducing differentiation into retinal cells until cells expressing the Rx gene begin to appear.
- Culture is performed to obtain adhesive aggregates containing retinal progenitor cells.
- the aggregate containing the retinal progenitor cells is subsequently subjected to adhesion culture in a serum-free medium or a serum medium until a retinal tissue is formed to obtain an adhesive aggregate containing the retinal tissue.
- adhesion of adhesion aggregates comprising retinal progenitor cells until 10% or more of the cells (preferably 20% or more, 30% or more, 40% or more, 50% or more) are in a state expressing Chx10. Culture is performed.
- retinal cells and retinal tissues can be obtained from pluripotent stem cells with high efficiency. Since the retinal tissue obtained by the method of the present invention includes neurons (neural cells) specific to each of the retinal layers, photoreceptor cells, horizontal cells, bipolar cells, amacrine cells, retinal ganglion cells, or these It is also possible to obtain various cells constituting retinal tissue such as progenitor cells. Which cells are obtained from the obtained retinal tissue can be confirmed by a method known per se, for example, expression of a cell marker.
- the obtained aggregate containing the retinal tissue may be used as it is as a reagent for toxicity / drug efficacy evaluation.
- Aggregates containing retinal tissue are dispersed (for example, trypsin / EDTA treatment), and the cells obtained are sorted using FACS or MACS to obtain high-purity retinal tissue constituent cells, for example, high-purity photoreceptors. It is also possible to obtain.
- the ciliary body peripheral portion-like structure can be produced from the cell aggregate containing retinal tissue obtained by the production method of the present invention by the following steps (A) and (B).
- the ciliary body periphery-like structure is a structure similar to the ciliary body periphery.
- “Ciliary marginal zone (CMZ)” is, for example, a tissue existing in a boundary region between a retinal tissue (specifically, a neural retina) and a retinal pigment epithelium in a biological retina, and A region containing retinal tissue stem cells (retinal stem cells) can be mentioned.
- the ciliary periphery is also called the ciliary margin or the retinal margin, and the ciliary margin, ciliary margin and retinal margin are equivalent tissues.
- the peripheral part of the ciliary body plays an important role in supplying retinal progenitor cells and differentiated cells to the retinal tissue, maintaining the retinal tissue structure, and the like.
- the marker gene for the ciliary margin include the Rdh10 gene (positive), the Otx1 gene (positive), and the Zic1 gene (positive).
- step (A) a cell aggregate containing a retinal tissue obtained by the production method of the present invention, and a cell aggregate in which the presence ratio of Chx10 positive cells in the retinal tissue is 20% or more and 100% or less.
- a serum-free medium or serum medium containing a Wnt signal transduction pathway agent and / or an FGF signal transduction pathway inhibitor the cells are cultured only during a period until cells expressing the RPE65 gene appear.
- a floating culture can be exemplified.
- a serum-free medium in which N2 or KSR is added to a basal medium can be mentioned. More specifically, N2 supplement (N2, Life Technologies) is added to a DMEM / F12 medium. And a serum-free medium supplemented with Examples of the serum medium include a serum medium obtained by adding fetal calf serum to a basal medium.
- Culture temperature in step (A), culturing conditions such as the CO 2 concentration may be set as appropriate.
- the culture temperature include a range of about 30 ° C. to about 40 ° C.
- the CO 2 concentration include a range of about 1% to about 10%.
- Preferably, for example, about 5% can be mentioned.
- the Wnt signaling pathway agent contained in the medium is mediated by Wnt.
- Wnt signaling pathway agonists include, for example, proteins belonging to the Wnt family (for example, Wnt1, Wnt3a, Wnt7a), Wnt receptors, Wnt receptor agonists, GSK3 inhibitors (for example, 6-Bromoindirubin-3 ′ -oxime (BIO), CHIR99021, Kenpaullone) and the like.
- the concentration of the Wnt signal transduction pathway agonist contained in the serum-free medium or serum medium of step (A) is, for example, about 0.1 ⁇ M to about 100 ⁇ M in the case of a normal Wnt signal transduction pathway agonist such as CHIR99021.
- a range can be mentioned.
- a range of about 1 ⁇ M to about 30 ⁇ M can be mentioned.
- a concentration of around 3 ⁇ M can be mentioned.
- the FGF signal transduction pathway inhibitor included in the medium is FGF-mediated signal transduction.
- FGF signal transduction pathway inhibitors include FGF receptors, FGF receptor inhibitors (eg, SU-5402, AZD4547, BGJ398), MAP kinase cascade inhibitors (eg, MEK inhibitors, MAPK inhibitors, ERK inhibition) Agent), PI3 kinase inhibitor, Akt inhibitor and the like.
- the concentration of the FGF signal transduction pathway inhibitor contained in the serum-free medium or serum medium in step (A) may be any concentration that can induce differentiation of the aggregate into a ciliary marginal-like structure.
- concentration of the FGF signal transduction pathway inhibitor contained in the serum-free medium or serum medium in step (A) may be any concentration that can induce differentiation of the aggregate into a ciliary marginal-like structure.
- it is added at a concentration of about 0.1 ⁇ M to about 100 ⁇ M, preferably about 1 ⁇ M to about 30 ⁇ M, more preferably about 5 ⁇ M.
- step (A) “culturing only during the period until cells expressing the RPE65 gene appear” means that all or part of the period until cells expressing the RPE65 gene appear It means to cultivate as long as possible.
- the “cell aggregates containing retinal tissue” present in the culture system is cultured only for the whole period or a part thereof (arbitrary period) composed of cells that do not substantially express the RPE65 gene. What is necessary is just to employ
- the above-mentioned “cell aggregate including retinal tissue” is used as a sample, and the presence or absence of the RPE65 gene contained in the sample or the degree thereof is determined by a normal genetic engineering technique or a living What is necessary is just to measure using a chemical method. Specifically, for example, the presence or absence of RPE65 gene expression or the degree thereof can be examined by a method of immunostaining a frozen section of the “cell aggregate containing retinal tissue” using an antibody against RPE65 protein.
- Examples of the “period until the appearance of cells expressing RPE65 gene” in the step (A) include, for example, the presence ratio of Chx10 positive cells in the retinal tissue is a Wnt signaling pathway agonist and / or FGF signaling pathway Examples include a period from the start of culturing of the cell aggregate in a serum-free medium or serum medium containing an inhibitor to a range of 30% to 0%. Examples of “cell aggregates in which cells expressing RPE65 gene do not appear” include cell aggregates in which the abundance of Chx10 positive cells in the retinal tissue is in the range of 30% to 0%.
- the number of days for the period until the appearance of cells expressing RPE65 gene in step (A) is the type of Wnt signal transduction pathway agonist and / or FGF signal transduction pathway inhibitor, the type of serum-free medium or serum medium Although it varies depending on other culture conditions, for example, it may be within 14 days. More specifically, when a serum-free medium (for example, a serum-free medium in which N2 is added to a basal medium) is used, the period can be preferably, for example, within 10 days, more preferably For example, 3 to 6 days can be mentioned.
- a serum-free medium for example, a serum-free medium in which N2 is added to a basal medium
- the period can be preferably, for example, within 12 days, and more preferably, for example, from 6 days. 9 days can be mentioned.
- a “cell aggregate in which cells expressing RPE65 gene do not appear” obtained by culturing as described above, a serum-free medium containing no Wnt signaling pathway agent or Culture in serum medium.
- Examples of preferable culture in the step (B) include suspension culture.
- Examples of the serum-free medium in step (B) include a medium in which N2 or KSR is added to the basal medium.
- Examples of the serum medium include a medium in which fetal calf serum is added to a basal medium, and more specifically, a serum medium in which fetal bovine serum is added to a DMEM / F12 medium.
- a known growth factor, an additive that promotes growth, a chemical substance, or the like may be added to the serum-free medium or serum medium in step (B).
- Known growth factors include EGF, FGF, IGF, insulin and the like.
- additives that promote proliferation include N2Nsupplement (manufactured by Life Technologies), B27 supplement (manufactured by Life Technologies), KSR, and the like.
- chemical substances that promote proliferation include retinoids (for example, retinoic acid) and taurine.
- a preferable culture time of the step (B) is, for example, the start of culture of the cell aggregate in a serum-free medium or serum medium in which the presence ratio of Chx10 positive cells in the retinal tissue does not contain a Wnt signaling pathway agent Examples of the incubation time can be given until the time increases to 30% or more.
- the culture conditions such as the culture temperature and CO 2 concentration in the step (B) may be set as appropriate.
- the culture temperature include a range of about 30 ° C. to about 40 ° C.
- the CO 2 concentration include a range of about 1% to about 10%, and preferably about 5%, for example.
- the above culture days until the step (B) “cell aggregates containing ciliary rim-like structures” are obtained vary depending on the type of serum-free medium or serum medium, other culture conditions, etc. For example, it can be listed within 100 days.
- the culture period is preferably 20 days to 70 days, and more preferably 30 days to 60 days, for example.
- the retinal pigment epithelium and the ciliary marginal portion-like structure are adjacent to each other.
- the retinal tissue (specifically, the neural retina) exists in the same cell aggregate.
- the structure can be confirmed by microscopic observation or the like. Specifically, for example, a ciliary peripheral edge-like structure formed as an epithelial structure formed between a highly transparent retinal tissue and a retinal pigment epithelium where pigmentation can be seen by microscopic observation as a thick retinal side and a thin retinal pigment epithelium side.
- the presence of the body can be confirmed.
- the presence of the ciliary peripheral portion-like structure can be confirmed by immunostaining the frozen section of the aggregate as Rdh10 positive, Otx1 positive, or Zic1 positive.
- Retinal pigment epithelial cells can be produced from the cell aggregate containing retinal tissue obtained by the production method of the present invention by the following step (C). From the retinal pigment epithelial cells obtained in the following step (C), a retinal pigment epithelial sheet can be produced by the following step (D).
- retinal pigment epithelial cell means an epithelial cell existing outside the neural retinal tissue in the living retina. If it is a person skilled in the art, whether it is a retinal pigment epithelial cell, for example, expression of cell markers (RPE65 (mature retinal pigment epithelial cell), Mitf (young or mature retinal pigment epithelial cell), etc.), melanin granules And the characteristic cell shape of the polygon.
- RPE65 mature retinal pigment epithelial cell
- Mitf youngng or mature retinal pigment epithelial cell
- a cell aggregate containing a retinal tissue obtained by the production method 2 of the present invention a serum-free medium or serum medium containing no WMP signal transduction pathway agonist but containing a BMP signal transduction pathway agonist
- the suspension is cultured in the medium to obtain aggregates containing retinal pigment epithelial cells.
- serum-free medium used in step (C) examples include serum-free medium in which N2 or KSR is added to the basal medium. More specifically, N2 supplement (Life Technologies) is added to the DMEM / F12 medium. Mention may be made of added serum-free media.
- the serum medium include a serum medium obtained by adding fetal calf serum to a basal medium.
- the serum-free medium used in step (C) may contain the aforementioned Nodal / Activin signaling pathway agonist and / or the aforementioned FGF signaling pathway inhibitor in addition to the aforementioned Wnt signaling pathway agonist. .
- a suspension culture can be exemplified.
- step (D) in which the aggregate obtained in the step (C) is dispersed and the resulting cells are adhered and cultured will be described.
- Step (D) is performed within 60 days, preferably within 30 days, more preferably 3 days after the start of implementation of step (C).
- Examples of the serum-free medium or serum medium used for the adhesion culture in the step (D) include the above-mentioned media.
- a serum-free medium supplemented with an appropriate amount of a commercially available serum substitute such as KSR for example, a 1/2 x N2 supplement in a 1: 1 mixture of DMEM / F-12 and Neurobasal, 1 / It is preferable to use a medium supplemented with 2 x B27 supplement and 100 ⁇ M 2-mercaptoethanol).
- KSR for example, a 1/2 x N2 supplement in a 1: 1 mixture of DMEM / F-12 and Neurobasal, 1 /
- 2 x B27 supplement and 100 ⁇ M 2-mercaptoethanol for example, in the case of human iPS cell-derived cells, the amount of KSR added to the serum-free medium is usually about 1% to about 20%, preferably about 2% to about 20%.
- the cells are preferably cultured in the above-described serum-free medium or serum medium containing the ROCK inhibitor.
- a serum-free medium further comprising one or more substances selected from the group consisting of a Wnt signaling pathway agonist, an FGF signaling pathway inhibitor, an Activin signaling pathway agonist and a BMP signaling pathway agonist or More preferably, the cells are cultured in serum medium.
- Activin signal transduction pathway agonist is a substance that can enhance the signal mediated by Activin.
- Activin signal transduction pathway agonist examples include proteins belonging to the Activin family (for example, Activin A, Activin B, Activin C, Activin AB, etc.), Activin receptor, and Activin receptor agonist.
- the concentration of the Activin signal transduction pathway agent used in step (D) may be any concentration that can efficiently form a uniform sheet of retinal pigment epithelial cells.
- concentration in the case of Recombinant Human / Mouse / Rat Activin A (R & D systems # 338-AC), about 1 ng / ml to about 10 ⁇ g / ml, preferably about 10 ng / ml to about 1 ⁇ g / ml, more preferably about 100 ng / ml Add so that the concentration becomes.
- Activin signal transduction pathway agonist is added, for example, within 18 days from the start of step (D), preferably on the 6th day.
- step (D) it is preferable to perform adhesion culture on the culture equipment surface-treated with the culture substrate.
- the culture substrate used for the treatment of the culture equipment in the step (D) include a cell culture substrate that enables adhesion culture of aggregate-derived cells and formation of a retinal pigment epithelial sheet.
- Retinal tissue or retinal cells (eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells) produced by the production method of the present invention are diseases of diseases based on retinal tissue or retinal cell damage. Since it is useful as a disease research material and drug discovery material in screening for therapeutic drugs and toxicity evaluation, it can be used as a reagent for evaluating the toxicity and efficacy of a test substance.
- an iPS cell is prepared from a human patient having a disease based on a disorder of retinal tissue, particularly a disease based on a hereditary disorder, and the iPS cell is used to produce retinal tissue or retinal cell (eg, visual field).
- the present invention contacts a test substance with retinal tissue or retinal cells (eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells) produced by the production method of the present invention, and the substance comprises the cells.
- retinal tissue or retinal cells eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells
- the present invention provides a method for evaluating toxicity / drug efficacy of the substance, which comprises assaying the influence on the tissue.
- a retinal tissue or retinal cell eg, photoreceptor cell, retinal progenitor cell, retinal layer-specific nerve cell
- a specific disorder eg, hereditary disorder
- a test substance with a reduced degree of the disorder can be selected as a candidate substance for a therapeutic drug for a disease based on the disorder.
- a test substance that further improves the physiological activity (for example, survival promotion or maturation) of retinal cells produced by the production method of the present invention can be searched for as a drug candidate substance.
- an induced pluripotent stem cell is prepared from a somatic cell having a genetic mutation exhibiting a specific disorder such as a disease accompanied by a retinal disorder, and the cell is covered with a retinal cell produced by inducing differentiation by the production method of the present invention.
- a test substance candidate effective as a therapeutic / preventive drug for the disorder can be searched by adding a test substance and using whether or not the disorder is present as an index.
- retinal tissue or retinal cells (eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells) produced by the production method of the present invention are used in the presence or absence of a test substance (negative). Culture in control). Then, the degree of toxicity in the retinal tissue or retinal cells treated with the test substance is compared with the negative control. As a result, the test substance showing toxicity compared to the negative control is determined as a substance having toxicity to retinal tissue or retinal cells (eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells). I can do it.
- the present invention includes a toxicity evaluation method including the following steps.
- Step 1 A step of measuring the degree of cell damage after culturing retinal tissue or retinal cells produced by the production method of the present invention in a viable culture condition in the presence of a test substance for a certain period of time.
- Step 2 After culturing the retinal tissue or retinal cells produced by the production method of the present invention for a certain period of time in the absence of a test substance or in the presence of a positive control under viable culture conditions, A step of measuring the degree of injury, and a step of evaluating the toxicity of the test substance in step 1 based on the difference in the results measured in (step 3) (step 1) and (step 2).
- test substance in the absence of the test substance includes adding only the culture solution and the solvent dissolving the test substance instead of the test substance.
- positive control means a known compound having toxicity.
- a method for measuring the degree of cell damage a method for measuring the number of living cells, for example, a method for measuring the amount of intracellular ATP, or a method for measuring the number of living cells by cell staining (for example, cell nucleus staining) and morphological observation. Methods and the like.
- step 3 as a method for evaluating the toxicity of the test substance, for example, the measured value of step 1 is compared with the measured value of the negative control in step 2, and when the degree of cell damage in step 1 is large, It can be determined that the test substance is toxic. Further, the measured value of the step 1 is compared with the measured value of the positive control in the step 2, and it can be determined that the test substance has toxicity when the degree of cell damage in the step 1 is equal to or higher than that.
- compositions comprising an effective amount of retinal tissue or retinal cells (eg, photoreceptor cells, retinal progenitor cells, retinal layer specific nerve cells) produced by the production method of the present invention.
- retinal tissue or retinal cells eg, photoreceptor cells, retinal progenitor cells, retinal layer specific nerve cells
- the pharmaceutical composition comprises an effective amount of retinal tissue or retinal cells (eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells) produced by the production method of the present invention, and a pharmaceutically acceptable carrier. .
- retinal tissue or retinal cells eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells
- a physiological aqueous solvent physiological saline, buffer solution, serum-free medium, etc.
- a preservative, a stabilizer, a reducing agent, an isotonic agent, and the like that are usually used may be added to the medicine containing the tissue or cells to be transplanted.
- the pharmaceutical composition of the present invention can be produced as a suspension by suspending the retinal tissue or retinal cells produced by the production methods 1 and 2 with an appropriate physiological aqueous solvent. If necessary, a cryopreservation agent may be added, cryopreserved, thawed at the time of use, washed with a buffer solution, and used for transplantation medicine.
- the retinal tissue obtained by the production method of the present invention can be cut into an appropriate size using tweezers or the like to form a sheet.
- the cells obtained by the production method of the present invention can be formed into sheet-like cells by adhesion culture in the step (3) of inducing differentiation to obtain a sheet agent.
- the pharmaceutical composition of the present invention is useful as a therapeutic agent for a disease based on a disorder of nerve tissue or nervous system cells (eg, retinal tissue, retinal progenitor cells, retinal layer-specific nerve cells).
- nerve tissue or nervous system cells eg, retinal tissue, retinal progenitor cells, retinal layer-specific nerve cells.
- Retinal tissues or retinal cells (eg, photoreceptor cells, retinal tissues, retinal progenitor cells, retinal layer-specific nerve cells) produced by the production method of the present invention are diseases that are based on disorders of the retinal tissues or retinal cells.
- Useful for transplantation medicine. Therefore, the present invention is based on a disorder of the retinal tissue or retinal cells, including retinal tissue or retinal cells (eg, photoreceptor cells, retinal progenitor cells, retinal layer-specific nerve cells) manufactured by the manufacturing method of the present invention.
- Provide therapeutics for diseases are diseases that are based on disorders of the retinal tissues or retinal cells.
- the retinal tissue or retinal cell produced by the production method of the present invention as a therapeutic agent for a disease based on a disorder of the retinal tissue or retinal cell, or in order to replenish the corresponding damaged site in the damaged state of the retinal tissue (Including retinal progenitor cells and retinal layer-specific neurons) can be used.
- a retinal tissue or retinal cell produced by the production method of the present invention is transplanted to a patient in need of transplantation, a disease based on a disorder of retinal tissue or retinal cell, or a damaged state of retinal tissue, and the retinal cell, By replenishing the damaged retinal tissue itself, diseases based on retinal tissue or retinal cell damage or retinal tissue damage can be treated.
- diseases based on retinal tissue or retinal cell disorders include retinal degeneration, retinitis pigmentosa, age-related macular degeneration, organic mercury poisoning, chloroquine retinopathy, glaucoma, diabetic retinopathy, and neonatal retinopathy. Can be mentioned.
- pluripotent stem cells eg, induced pluripotent stem cells
- pluripotent stem cells established from recipient somatic cells eg, induced pluripotent stem cells
- autologous neural tissue or nervous system cells are produced and transplanted into the recipient.
- pluripotent stem cells eg, induced pluripotent stem cells
- somatic cells that are compatible with the recipient's immunity (for example, compatible with HLA type or MHC type)
- allo neural tissue or nerve Lineage cells may be produced and transplanted into the recipient.
- Comparative Example 1 Example of differentiation from human iPS cells without using an Shh signaling pathway agent in Step 2 Human iPS cells (1231A3 strain, obtained from Kyoto University) described in Scientific Reports, 4, 3594 (2014) According to the method, the cells were cultured under feeder-free conditions.
- StemFit medium (AK03, manufactured by Ajinomoto Co., Inc.) was used as the feeder-free medium, and Laminin511-E8 (Nippi Co., Ltd.) was used as the feeder-free scaffold.
- human iPS cells (1231A3 strain) which became sub-confined were first washed with PBS and then dispersed into single cells using TrypLE Select (Life Technologies). Thereafter, the human iPS cells dispersed in the single cells were seeded in a plastic culture dish coated with Laminin511-E8, and feeder-free culture was performed in StemFit medium in the presence of Y27632 (ROCK inhibitor, 10 ⁇ M).
- a 6-well plate manufactured by Iwaki, for cell culture, culture area 9.4 cm 2
- the number of seeded cells of human iPS cells dispersed in the single cells was 6 ⁇ 10 3 did.
- the entire medium was replaced with StemFit medium not containing Y27632. Thereafter, the whole medium was exchanged with StemFit medium not containing Y27632 once or twice a day. Then, 6 days after seeding, the cells were cultured until they became sub-confluent (about 60% of the culture area was covered with cells).
- the subconfluent human iPS cells were treated with a cell dispersion using TrypLE Select (Life Technologies), and further dispersed into single cells by pipetting. Thereafter, 100 ⁇ l of human iPS cells dispersed in the single cells were added to a non-cell-adhesive 96-well culture plate (PrimeSurface 96V bottom plate, Sumitomo Bakelite Co., Ltd.) at 1.2 ⁇ 10 4 cells per well. The suspension was suspended in a serum-free medium and cultured in suspension at 37 ° C. and 5% CO 2 .
- the serum-free medium (gfCDM + KSR) is a serum-free medium containing 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1x chemically defined lipid concentrate in a 1: 1 mixture of F-12 medium and IMDM medium.
- Y27632 final concentration 20 ⁇ M was added to the serum-free medium at the start of suspension culture (day 0 after suspension culture started, step 2 started). Cell aggregates were formed by the second day after the start of suspension culture (end of step 2 and start of step 3). On day 3 after the start of suspension culture, 50 ⁇ l of serum-free medium without Y27632 was added.
- the final concentration of exogenous human recombinant BMP4 was 1.5 nM (55 ng / ml, figure) in medium containing or not containing Y27632 and human recombinant BMP4 (R & D). 1B) or a half amount of the medium was changed so that the medium does not contain exogenous human recombinant BMP4 (FIG. 1A).
- the medium in the incubator was discarded by half the volume, ie, 75 ⁇ l, and 75 ⁇ l of fresh serum-free medium was added, so that the total volume of the medium was 150 ⁇ l.
- Example 1 Example of formation of cell aggregate, nerve tissue and retinal tissue prepared from a human iPS cell cultured using StemFit as a feeder-free medium in Step 1 using a Shh transmission pathway agent in Step 2
- Human iPS Cells (1231A3 strain, obtained from Kyoto University) were cultured under feeder-free conditions according to the method described in Scientific Reports, 4, 3594 (2014).
- StemFit medium (AK03, manufactured by Ajinomoto Co., Inc.) was used as the feeder-free medium, and Laminin511-E8 (Nippi Co., Ltd.) was used as the feeder-free scaffold.
- the maintenance culture operation was performed by the method described in Comparative Example 1.
- the subconfluent human iPS cells prepared in this way were treated with cell dispersion using TrypLE Select (Life Technologies), and further dispersed into single cells by pipetting. Thereafter, 100 ⁇ l of human iPS cells dispersed in the single cells were added to a non-cell-adhesive 96-well culture plate (PrimeSurface 96V bottom plate, Sumitomo Bakelite Co., Ltd.) at 1.2 ⁇ 10 4 cells per well. The suspension was suspended in a serum-free medium and cultured in suspension at 37 ° C. and 5% CO 2 .
- the serum-free medium (gfCDM + KSR) is serum-free with 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1 x Chemically defined lipid concentrate in a 1: 1 mixture of F-12 medium and IMDM medium. Medium was used.
- Y27632 final concentration 20 ⁇ M
- SAG 300 nM
- Cell aggregates were formed by the second day after the start of suspension culture (end of step 2 and start of step 3).
- 50 ⁇ l of serum-free medium without Y27632 and SAG was added.
- the final concentration of exogenous human recombinant BMP4 was 1.5 nM (55 ng / ml, figure) in a medium that did not contain Y27632 and contained or did not contain human recombinant BMP4 (R & D). 1D) or a half amount of the medium was changed so that the medium does not contain exogenous human recombinant BMP4 (FIG. 1C).
- the medium in the incubator was discarded by half the volume, ie, 75 ⁇ l, and 75 ⁇ l of fresh serum-free medium was added, so that the total volume of the medium was 150 ⁇ l.
- the cell aggregates on the 19th day after the start of suspension culture were each fixed with 4% paraformaldehyde to prepare frozen sections. Regarding these frozen sections, one of the retinal tissue markers, Chx10 (anti-Chx10 antibody, Exalpha, sheep), or one of the retinal tissue markers, Rx (anti-Rx antibody, TAKARA, Guinea Pig) Immunostaining was performed. In the immunostaining analysis, when determining whether or not the marker is positive, it was determined to be positive if the luminance value was two or more times higher than the background.
- the ratio of Rx-positive cells in all cells was 3 under the conditions in which the Shh signaling pathway agonist was added on day 0 of suspension culture and the BMP signaling pathway agent was not added on day 6 of suspension culture. It was found that the percentage of Chx10 positive cells was less than 3% (FIGS. 2A and 2C). On the other hand, in the condition where the Shh signaling pathway agonist was added on the 0th day of suspension culture and the BMP signaling pathway agonist was added on the 6th day of suspension culture, the ratio of Rx positive cells in all cells was 60% or more, The ratio of Chx10 positive cells in all cells was also found to be 60% or more (FIGS. 2B and D).
- Example 2 Example of production of neural tissue using Shh transmission pathway agonist at the start of suspension culture from human iPS cells using Essential 8 as feeder-free medium in step 1
- Human iPS cells (1231A3 strain, obtained from Kyoto University) was cultured under feeder-free conditions according to the method described in “Scientific Reports, 4, 3594 (2014)”.
- Essential 8 medium Life Technologies
- Laminin511-E8 Nappi was used as the feeder-free scaffold.
- human iPS cells (1231A3 strain) which became sub-confined were first washed with PBS and then dispersed into single cells using TrypLE Select (Life Technologies). Thereafter, human iPS cells dispersed in the single cells were seeded on a plastic culture dish coated with Laminin511-E8, and feeder-free culture was performed in Essential 8 medium in the presence of Y27632 (ROCK inhibitor, 10 ⁇ M). .
- a 6-well plate manufactured by Iwaki, for cell culture, culture area 9.4 cm 2
- the number of seeded cells of human iPS cells dispersed in the single cells was 6 ⁇ 10 3 did.
- the entire medium was replaced with Essential 8 medium not containing Y27632. Thereafter, the entire medium was changed once a day or two with an Essential 8 medium not containing Y27632. Then, 6 days after seeding, the cells were cultured until they became sub-confluent (about 60% of the culture area was covered with cells).
- the subconfluent human iPS cells thus prepared were treated with a cell dispersion using TrypLE Select (manufactured by Life Technologies), and further dispersed into single cells by pipetting. Thereafter, 100 ⁇ l of the human iPS cells dispersed in the single cells are added to a non-cell-adhesive 96-well culture plate (PrimeSurface 96V bottom plate, manufactured by Sumitomo Bakelite Co., Ltd.) at 1.2 ⁇ 10 4 cells per well. Were suspended in a serum-free medium and suspended in culture at 37 ° C. and 5% CO 2 .
- the serum-free medium (gfCDM + KSR) is serum-free with 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1 x Chemically defined lipid concentrate in a 1: 1 mixture of F-12 medium and IMDM medium. Medium was used.
- a serum-free medium in which Y27632 (final concentration 20 ⁇ M) was added to the serum-free medium was used (FIGS. 3A-D).
- a serum-free medium was used in which Y27632 (final concentration 20 ⁇ M) and SAG (300 nM) as a Shh signaling pathway agonist were added to the serum-free medium (FIGS.
- Example 3 Example of production of retinal tissue using human Shh pathway acting substance at the start of suspension culture from human iPS cells using Essential 8 as feeder-free medium in step 1
- Human iPS cells (201B7 strain, obtained from Kyoto University) ) was feeder-free cultured according to the method described in Scientific Reports, 4, 3594 (2014).
- Essential 8 medium Life Technologies
- Laminin511-E8 Nappi was used as the feeder-free scaffold.
- human iPS cells (201B7 strain) which became sub-confined were first washed with PBS and then dispersed into single cells using TrypLE Select (manufactured by Life Technologies). Thereafter, human iPS cells dispersed in the single cells were seeded on a plastic culture dish coated with Laminin511-E8, and feeder-free culture was performed in Essential 8 medium in the presence of Y27632 (ROCK inhibitor, 10 ⁇ M). .
- a 6-well plate manufactured by Iwaki, for cell culture, culture area 9.4 cm 2
- the number of seeded cells of human iPS cells dispersed in the single cells was 6 ⁇ 10 3 did.
- the entire medium was replaced with Essential 8 medium not containing Y27632. Thereafter, the entire medium was changed once a day or two with an Essential 8 medium not containing Y27632. Then, 6 days after seeding, the cells were cultured until they became sub-confluent (about 60% of the culture area was covered with cells).
- the subconfluent human iPS cells thus prepared were treated with a cell dispersion using TrypLE Select (manufactured by Life Technologies), and further dispersed into single cells by pipetting. Thereafter, 100 ⁇ l of human iPS cells dispersed in the single cells were added to a non-cell-adhesive 96-well culture plate (PrimeSurface 96V bottom plate, Sumitomo Bakelite Co., Ltd.) at 1.2 ⁇ 10 4 cells per well. The suspension was suspended in a serum-free medium and cultured in suspension at 37 ° C. and 5% CO 2 .
- the serum-free medium (gfCDM + KSR) is a serum-free medium containing 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1xChemically defined lipid concentrate in a 1: 1 mixture of F-12 medium and IMDM medium.
- the cell aggregate on the 18th day after the start of the suspension culture was fixed with 4% paraformaldehyde to prepare a frozen section.
- one of the retinal tissue markers, Chx10 (anti-Chx10 antibody, Exalpha, sheep), or one of the retinal tissue markers, Rx (anti-Rx antibody, TAKARA, Guinea Pig) Immunostaining was performed.
- the ratio of Rx-positive cells in all cells was 3 under the conditions in which the Shh signaling pathway agonist was added on day 0 of suspension culture and the BMP signaling pathway agent was not added on day 6 of suspension culture.
- % And the proportion of Chx10 positive cells in all cells was 3% or less (FIGS. 5A and 5C).
- the ratio of Rx positive cells in all cells was 40% or more
- the ratio of Chx10 positive cells in all cells was also found to be 40% or more (FIGS. 5B and D).
- analysis of serial sections revealed that Rx was also strongly positive in nerve tissues where the proportion of Chx10 positive cells was high (95% or more) (FIGS. 5B and D).
- Example 4 Example of production of retinal tissue from human iPS cells using Shh transfer pathway agonist in step 2 and BMP4 in step 3 Human iPS cells (1231A3 strain, obtained from Kyoto University), Scientific Reports, 4 , 3594 (2014).
- StemFit medium (AK03, manufactured by Ajinomoto Co., Inc.) was used as the feeder-free medium, and Laminin511-E8 (Nippi Co., Ltd.) was used as the feeder-free scaffold.
- human iPS cells (1231A3 strain) which became sub-confined were first washed with PBS and then monodispersed using TrypLE Select (manufactured by Life Technologies). Thereafter, the monodispersed human iPS cells were seeded on a plastic culture dish coated with Laminin511-E8, and feeder-free culture was performed in StemFit medium in the presence of Y27632 (ROCK pathway inhibitor, 10 ⁇ M).
- Y27632 ROCK pathway inhibitor, 10 ⁇ M
- the number of seeded cells of the monodispersed human iPS cells was 6 ⁇ 10 3 .
- the stemFit medium without Y27632 was replaced. Thereafter, the medium was changed once a day or two days with a StemFit medium not containing Y27632. Then, 6 days after seeding, the cells were cultured until they became sub-confluent (about 60% of the culture area was covered with cells).
- the subconfluent human iPS cells were monodispersed using TrypLE Select (Life Technologies), and then the monodispersed human iPS cells were non-cell-adhesive 96-well culture plate (PrimeSurface 96V bottom). Plate, Sumitomo Bakelite Co., Ltd.) was suspended in 100 ⁇ l of serum-free medium at 1.2 ⁇ 10 4 cells per well and cultured in suspension at 37 ° C. and 5% CO 2 .
- the serum-free medium (gfCDM + KSR) is serum-free with 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1 x Chemically defined lipid concentrate in a 1: 1 mixture of F-12 medium and IMDM medium. Medium was used.
- Y27632 final concentration 20 ⁇ M
- SAG final concentration 300 nM
- Condition 1 (+ BMP, d3) is that, on the third day after the start of suspension culture, the final concentration of exogenous human recombinant BMP4 is 1.5 nM, which does not contain Y27632 and SAG, and human recombinant BMP4 (R & D) 50 ⁇ l of medium containing (manufactured) was added (total amount of medium 150 ⁇ l). On the 6th day after the start of suspension culture, half of the medium was replaced with a medium not containing Y27632, SAG and human recombinant BMP4.
- condition 2 (+ BMP, d6), 50 ⁇ l of serum-free medium not containing Y27632, SAG and human recombinant BMP4 was added on the third day after the start of suspension culture. On the 6th day after the start of suspension culture, half of the medium was exchanged so that the final concentration of exogenous human recombinant BMP4 was 1.5 nM in a medium containing human recombinant BMP4 (R & D) without Y27632 and SAG. .
- Condition 1 and Condition 2 were exchanged by half in the serum-free medium containing no Y27632, SAG, and human recombinant BMP4 once every 2 to 4 days after the start of suspension culture. On the 18th day after the start of suspension culture, the morphology was observed with an inverted microscope. As a result, it was found that cell aggregates were maintained and nerve tissue was formed in both condition 1 and condition 2.
- Example 5 Example of production of retinal tissue from human iPS cells using 600 nM Shh transmission pathway agonist in step 2 and BMP4 in step 3 Feeder-free human iPS cells (1231A3) prepared by the method described in Example 4 Strain) using TrypLE Select (manufactured by Life Technologies), then the monodispersed human iPS cells were non-cell-adhesive 96-well culture plate (PrimeSurface 96V bottom plate, Sumitomo Bakelite) The suspension was suspended in 100 ⁇ l of serum-free medium so that the number of cells per well was 1.2 ⁇ 10 4 cells, and the suspension was cultured at 37 ° C. and 5% CO 2 .
- the serum-free medium (gfCDM + KSR) was serum-free with 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1 x Chemically defined lipid concentrate in a 1: 1 mixture of F-12 medium and IMDM medium. Medium was used. Y27632 (final concentration 20 ⁇ M) and SAG (final concentration 600 nM) were added to the serum-free medium at the start of suspension culture (day 0 after suspension culture start, step 2 started). Cell aggregates were formed by the second day after the start of suspension culture (end of step 2 and start of step 3).
- Condition 1 (+ BMP, d3) is that, on the third day after the start of suspension culture, the final concentration of exogenous human recombinant BMP4 is 1.5 nM, which does not contain Y27632 and SAG, and human recombinant BMP4 (R & D) 50 ⁇ l of medium containing (manufactured) was added (total amount of medium 150 ⁇ l). On the 6th day after the start of suspension culture, half of the medium was replaced with a medium not containing Y27632, SAG and human recombinant BMP4.
- condition 2 (+ BMP, d6), 50 ⁇ l of serum-free medium not containing Y27632, SAG and human recombinant BMP4 was added on the third day after the start of suspension culture. On the sixth day after the start of suspension culture, a half amount of the medium was exchanged with a medium containing human recombinant BMP4 (manufactured by R & D) without Y27632 so that the final concentration of exogenous human recombinant BMP4 was 1.5 nM.
- Condition 1 and Condition 2 were exchanged by half in the serum-free medium containing no Y27632, SAG, and human recombinant BMP4 once every 2 to 4 days after the start of suspension culture. On the 18th day after the start of suspension culture, the morphology was observed with an inverted microscope. As a result, it was found that cell aggregates were maintained and nerve tissue was formed in both condition 1 and condition 2.
- Example 6 Example of production of retinal tissue from human iPS cells using SAG, Purmorphamine, and recombinant Shh protein as sonic hedgehog signaling pathway agonists in step 2
- Human iPS cells (201B7 strain, obtained from Kyoto University) And feeder-free culture according to the method described in Scientific Reports, 4, 3594 (2014).
- Stem Fit medium (AK-03; manufactured by Ajinomoto Co., Inc.) was used as the feeder-free medium
- Laminin511-E8 Nappi Co., Ltd.
- human iPS cells (201B7 strain) which became sub-confined were first washed with PBS and then dispersed into single cells using TrypLE Select (manufactured by Life Technologies). Thereafter, human iPS cells dispersed in the single cells were seeded in a plastic culture dish coated with Laminin511-E8, and feeder-free culture was performed in Stem Fit medium in the presence of Y27632 (ROCK pathway inhibitor, 10 ⁇ M). did.
- Y27632 ROCK pathway inhibitor, 10 ⁇ M
- the whole medium was replaced with Stem Fit medium not containing Y27632. Thereafter, the whole medium was exchanged with Stem Fit medium not containing Y27632 once a day or two days. Then, 6 days after seeding, the cells were cultured until they became sub-confluent (about 60% of the culture area was covered with cells).
- the subconfluent human iPS cells thus prepared were treated with a cell dispersion using TrypLE Select (manufactured by Life Technologies), and further dispersed into single cells by pipetting. After that, human iPS cells dispersed in the single cells become 1.0 ⁇ 10 4 cells per well of a non-cell-adhesive 96-well culture plate (Sumilon Spheroid V bottom plate PrimeSurface 96V bottom plate, Sumitomo Bakelite) The suspension was suspended in 100 ⁇ l of serum-free medium and cultured at 37 ° C. in 5% CO 2 .
- serum-free medium (gfCDM + KSR) was prepared by adding 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1 x Chemically defined lipid concentrate to a 1: 1 mixture of F-12 medium and IMDM medium. Serum medium was used. At the start of suspension culture (0 days after suspension culture start, start of step 2), Y27632 (20 ⁇ M) was added to the serum-free medium, and SAG (final concentration 30 nM), Purmorphamine (Wako) (Final concentration 0.2 ⁇ M) or serum-free medium supplemented with human recombinant Shh (R & D, Recombinant N-Terminus, final concentration 50 ng / ml) was used (FIG. 8).
- the cell aggregate on the 18th day after the start of the suspension culture was fixed with 4% paraformaldehyde to prepare a frozen section.
- immunostaining was performed on Chx10 (anti-Chx10 antibody, manufactured by Exalpha Co., Ltd. sheep), which is one of retinal tissue markers.
- Chx10 anti-Chx10 antibody, manufactured by Exalpha Co., Ltd. sheep
- SAG, PMA, or Shh was added as a substance acting on the Shh signaling pathway on the 0th day of suspension culture and BMP4 was added on the 3rd day of suspension culture
- the ratio of Chx10 positive cells in all cells was also It was found to be 30% or more (FIG. 8, D to F).
- SAG, Purmorphamine, or recombinant Shh was added as a Shh signaling pathway agonist at the start of suspension culture in Step 2, and a BMP signaling pathway agonist as a differentiation inducer during suspension culture in Step 3. It was found that retinal tissue can be efficiently produced from human iPS cells cultured in a feeder-free manner.
- Example 7 Example of production of retinal tissue from human iPS cells using SAG, Purmorphamine and recombinant Shh protein as Sonic hedgehog signaling pathway agonists in step 2 and BMP4 in step 3
- Human iPS cells (1231A3 strain) Obtained from Kyoto University) was feeder-free cultured according to the method described in Scientific Reports, 4, 3594 (2014).
- Stem Fit medium (AK-03; manufactured by Ajinomoto Co., Inc.) was used as the feeder-free medium, and Laminin511-E8 (Nippi Co., Ltd.) was used as the feeder-free scaffold.
- Subconfluent human iPS cells prepared by the method described in Example 6 were treated with cell dispersion using TrypLE Select (manufactured by Life Technologies), and further dispersed into single cells by pipetting. After that, human iPS cells dispersed in the single cells become 1.0 ⁇ 10 4 cells per well of a non-cell-adhesive 96-well culture plate (Sumilon Spheroid V bottom plate PrimeSurface 96V bottom plate, Sumitomo Bakelite) The suspension was suspended in 100 ⁇ l of serum-free medium and cultured at 37 ° C. in 5% CO 2 .
- the serum-free medium (gfCDM + KSR) is a serum-free medium containing 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1xChemically defined lipid concentrate in a 1: 1 mixture of F-12 medium and IMDM medium. Was used.
- a medium that does not contain Y27632, SAG, Purmorphamine, and recombinant Shh but contains human recombinant BMP4 (manufactured by R & D) has a final concentration of exogenous human recombinant BMP4 of 1.5 nM. 50 ⁇ l was added to make (55 ng / ml).
- half of the serum-free medium containing no Y27632, SAG, Purmorphamine, Shh protein, and human recombinant BMP4 was replaced.
- Chx10 anti-Chx10 antibody, manufactured by Exalpha Co., Ltd. sheep
- BMP4 was added on the 3rd day of suspension culture (lower part of Fig. 9).
- Chx10 positive cells were contained in the cell aggregates even under the condition where SAG or Shh was added on the 0th day of suspension culture.
- Example 8 Human iPS cells established using Sendai virus, feeder-free culture, using an Shh signaling pathway agent at the start of suspension culture, and using BMP4 in Step 3, human iPS cells (DSPC-3 strain, established by Sumitomo Dainippon Pharma Co., Ltd.) is a commercially available Sendai virus vector (Oct3 / 4, Sox2, KLF4, c-Myc 4 factors, DNAVEC (now ID Pharma) site tune kit ), A public protocol from Life Technologies (iPS 2.0 Sendai Reprogramming Kit, Publication Number MAN0009378, Revision 1.0), and a public protocol from Kyoto University (feeder-free establishment and maintenance culture of human iPS cells, CiRA_Ff-iPSC_protocol_JP_v140310 , http://www.cira.kyoto-u.ac.jp/j/research/protocol.html), StemFit medium (AK03; Ajinomoto Co., Inc.), Laminin511-E8 (
- the human iPS cells were feeder-free cultured according to the method described in Scientific Reports, 4, 3594 (2014).
- Stem Fit medium (Ajinomoto Co., Inc.) was used as the feeder-free medium, and Laminin511-E8 (Nippi Co., Ltd.) was used as the feeder-free scaffold.
- the whole medium was replaced with Stem Fit medium not containing Y27632. Thereafter, the whole medium was exchanged with Stem Fit medium not containing Y27632 once a day or two days. Then, 6 days after seeding, the cells were cultured until they became sub-confluent (about 60% of the culture area was covered with cells).
- the subconfluent human iPS cells thus prepared were treated with a cell dispersion using TrypLE Select (manufactured by Life Technologies), and further dispersed into single cells by pipetting. After that, human iPS cells dispersed in the single cells become 1.2 ⁇ 10 4 cells per well of a non-cell-adhesive 96-well culture plate (Sumilon Spheroid V bottom plate PrimeSurface 96V bottom plate, Sumitomo Bakelite) The suspension was suspended in 100 ⁇ l of serum-free medium and cultured at 37 ° C. in 5% CO 2 .
- serum-free medium (gfCDM + KSR) was prepared by adding 10% KSR, 450 ⁇ M 1-monothioglycerol, and 1 x Chemically defined lipid concentrate to a 1: 1 mixture of F-12 medium and IMDM medium. Serum medium was used. Conditions for adding Y27632 (20 ⁇ M) to the serum-free medium and adding SAG as an exogenous Shh signaling pathway agent at the start of suspension culture (day 0 after suspension culture start, step 2 start) (final concentration 300 nM), or a serum-free medium under the condition that no SAG was added (final concentration 0 nM) (FIG. 10). On the second day after the start of suspension culture, cell aggregates were formed.
- the final concentration of exogenous human recombinant BMP4 is 1.5 nM (55 ng) in a medium that does not contain Y27632 and SAG and does not contain human recombinant BMP4 (R & D). / ml) or 50 ⁇ l was added to reach 0 nM.
- the serum-free medium containing no Y27632, SAG, and BMP4 was replaced by a half amount. Thereafter, once every 2 to 4 days, half of the medium was replaced with the above serum-free medium containing no Y27632, SAG, or BMP4.
- the cell aggregates thus prepared were subjected to bright field observation using an inverted microscope (manufactured by Keyence Corporation, BIOREVO) on the 22nd day after suspension culture was started. It was found that cell aggregate formation efficiency was poor under the conditions (FIG. 10, Control). On the other hand, it was found that cell aggregates were maintained and neuroepithelium was formed under the conditions in which SAG was added at the start of suspension culture (FIG. 10, SAG 300 nM).
- the cell aggregates on the 22nd day after the start of the suspension culture were fixed with 4% paraformaldehyde to prepare frozen sections. Regarding these frozen sections, one of the retinal tissue markers, Chx10 (anti-Chx10 antibody, Exalpha, sheep), or one of the retinal tissue markers, Rx (anti-Rx antibody, Takara, Guinea Pig) Immunostaining was performed. As a result, when SAG was added on the 0th day of suspension culture and BMP4 was added on the 3rd day of suspension culture, the ratio of Rx positive cells in all cells was about 10%, and the ratio of Chx10 positive cells in all cells was found to be around 10% (Fig. 10).
- the production method of the present invention makes it possible to produce retinal tissue by inducing differentiation from pluripotent stem cells to retinal cells in the absence of feeder cells.
- the production method of the present invention comprises a material for use in testing and treatment for evaluating the toxicity / drug efficacy of drug candidate compounds and other chemical substances using retinal tissue, and for application to a transplant material for retinal tissue transplantation treatment. This is useful in that the retinal tissue can be manufactured.
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Abstract
Description
これら製造法の出発材料である多能性幹細胞は、特に霊長類多能性幹細胞の場合、フィーダー細胞存在下・未分化維持因子添加条件で未分化維持培養されていた。近年、未分化維持培養の改良が進み、霊長類多能性幹細胞を、フィーダー細胞非存在下(フィーダーフリー)・未分化維持因子添加条件にて培養する手法が報告されている(非特許文献7、非特許文献8及び非特許文献9)。当該手法でフィーダーフリー培養された多能性幹細胞を出発材料として、網膜細胞又は網膜組織を安定的に製造する方法が切望されていた。
すなわち、本発明は以下に関する。
(1)ヒト多能性幹細胞を、フィーダー細胞非存在下で、未分化維持因子を含む培地で培養する第一工程、
(2)第一工程で得られた多能性幹細胞を、ソニック・ヘッジホッグシグナル伝達経路作用物質の存在下で浮遊培養し、細胞の凝集体を形成させる第二工程、及び
(3)第二工程で得られた凝集体を、BMPシグナル伝達経路作用物質の存在下に浮遊培養し、網膜細胞又は網膜組織を含む凝集体を得る第三工程。
[2]第二工程において、第一工程で得られた細胞を分散し、当該分散した細胞を浮遊培養する、[1]に記載の製造方法。
[3]未分化維持因子が、FGFシグナル伝達経路作用物質である[1]又は[2]に記載の製造方法。
[4]FGFシグナル伝達経路作用物質が、bFGFである、[3]に記載の製造方法。
[5]第二工程における培地中のソニック・ヘッジホッグシグナル伝達経路作用物質の濃度が、SAG10nM~700nMのソニック・ヘッジホッグシグナル伝達作用に相当する濃度である、[1]~[4]のいずれかに記載の製造方法。
[6]ソニック・ヘッジホッグシグナル伝達経路作用物質がSAG、Purmorphamine又はShhである、[1]~[5]のいずれかに記載の製造方法。
[7]BMPシグナル伝達経路作用物質が、BMP2、BMP4、BMP7及びGDF7からなる群から選ばれる1以上の蛋白質である、[1]~[6]のいずれかに記載の製造方法。
[8]BMPシグナル伝達経路作用物質が、BMP4である、[6]に記載の製造方法。
[9]第三工程において、第二工程の開始後2日目から9日目の間にBMPシグナル伝達経路作用物質が培地に添加される、[1]~[8]のいずれかに記載の製造方法。
[10]第三工程において、ソニック・ヘッジホッグシグナル伝達経路作用物質の濃度がSAG700nMのソニック・ヘッジホッグシグナル伝達作用に相当する濃度以下である培地で凝集体を培養する、[1]~[9]のいずれかに記載の製造方法。
[11]第一工程が接着培養法で行われる、[1]~[10]のいずれかに記載の製造方法。
[12]多能性幹細胞が人工多能性幹細胞である、[1]~[11]のいずれかに記載の製造方法。
[13]第二工程において、均一な凝集体を形成させる、[1]~[12]のいずれかに記載の製造方法。
[14]第三工程において得られる凝集体が、網膜前駆細胞、神経網膜前駆細胞、視細胞前駆細胞、視細胞、桿体視細胞、錐体視細胞、水平細胞、アマクリン細胞、介在神経細胞、神経節細胞、網膜色素上皮細胞、及び毛様体周縁部細胞からなる群から選択される1又は複数の細胞を含む凝集体である、[1]~[13]のいずれかに記載の製造方法。
[15]浮遊培養が、基底膜標品非存在下で行われる[1]~[14]のいずれかに記載の製造方法。
[16][1]~[15]のいずれかに記載の方法により製造される網膜細胞又は網膜組織を含有してなる、被検物質の毒性・薬効評価用試薬。
[17][1]~[15]のいずれかに記載の方法により製造される網膜細胞または網膜組織に被検物質を接触させ、該物質が該細胞又は該組織に及ぼす影響を検定することを含む、該物質の毒性・薬効評価方法。
[18][1]~[15]のいずれかに記載の方法により製造される網膜細胞又は網膜組織を含有してなる、網膜組織の障害に基づく疾患の治療薬。
[19]網膜細胞が網膜前駆細胞及び/又は網膜層特異的神経細胞である、[18]に記載の治療薬。
[20][1]~[15]のいずれかに記載の方法により製造される、網膜細胞又は網膜組織の有効量を、移植を必要とする対象に移植することを含む、網膜組織の障害に基づく疾患の治療方法。
[21]網膜組織の障害に基づく疾患の治療における使用のための[1]~[15]のいずれかに記載の方法により製造される網膜細胞又は網膜組織。
[22][1]~[15]のいずれかに記載の方法により製造される網膜細胞又は網膜組織を有効成分として含有する、医薬組成物。
本発明において、「幹細胞」とは、分化能及び分化能を維持した増殖能(特に自己複製能)を有する未分化な細胞を意味する。幹細胞には、分化能力に応じて、多能性幹細胞(pluripotent stem cell)、複能性幹細胞(multipotent stem cell)、単能性幹細胞(unipotent stem cell)等の亜集団が含まれる。多能性幹細胞とは、インビトロにおいて培養することが可能で、かつ、三胚葉(外胚葉、中胚葉、内胚葉)及び/又は胚体外組織由来の組織に属する細胞系譜すべてに分化しうる能力(分化多能性(pluripotency))を有する幹細胞をいう。複能性幹細胞とは、全ての種類ではないが、複数種の組織や細胞へ分化し得る能力を有する幹細胞を意味する。単能性幹細胞とは、特定の組織や細胞へ分化し得る能力を有する幹細胞を意味する。
また、株化された人工多能性幹細胞を入手する事も可能であり、例えば、京都大学で樹立された201B7細胞、201B7-Ff細胞、253G1細胞、253G4細胞、1201C1細胞、1205D1細胞、1210B2細胞又は、1231A3細胞等のヒト人工多能性細胞株が、京都大学及びiPSアカデミアジャパン株式会社より入手可能である。株化された人工多能性幹細胞として、例えば、京都大学で樹立されたFf-I01細胞及びFf-I14細胞は、京都大学より入手可能である。
本発明の製造方法は、下記工程(1)~(3)を含む、網膜細胞又は網膜組織の製造方法である:
(1)ヒト多能性幹細胞を、フィーダー細胞非存在下で、未分化維持因子を含む培地で培養する第一工程、
(2)第一工程で得られた多能性幹細胞を、ソニック・ヘッジホッグシグナル伝達経路作用物質の存在下で浮遊培養し、細胞の凝集体を形成させる第二工程、及び
(3)第二工程で得られた凝集体を、分化誘導因子の存在下もしくは非存在下に浮遊培養し、網膜細胞もしくは網膜組織を含む凝集体を得る第三工程。
して、毛様体周縁部様構造体の存在を確認することができる。
本発明の製造方法により製造された、網膜組織又は網膜細胞(例、視細胞、網膜前駆細胞、網膜層特異的神経細胞)は、網膜組織又は網膜細胞の障害に基づく疾患の治療薬のスクリーニングや、毒性評価における、疾患研究材料、創薬材料として有用であるので、被検物質の毒性・薬効評価用試薬とすることができる。例えば、網膜組織の障害に基づく疾患、特に遺伝性の障害に基づく疾患のヒト患者から、iPS細胞を作製し、このiPS細胞を用いて本発明の方法により、網膜組織又は網膜細胞(例、視細胞、網膜前駆細胞、網膜層特異的神経細胞)を製造する。網膜組織又は網膜細胞は、その患者が患っている疾患の原因となる網膜組織の障害をインビトロで再現し得る。そこで、本発明は、本発明の製造方法により製造される網膜組織又は網膜細胞(例、視細胞、網膜前駆細胞、網膜層特異的神経細胞)に被検物質を接触させ、該物質が該細胞又は該組織に及ぼす影響を検定することを含む、該物質の毒性・薬効評価方法を提供する。
(工程1)本発明の製造方法により製造された網膜組織又は網膜細胞を、生存可能な培養条件で、一定時間、被検物質の存在下で培養した後、細胞の傷害の程度を測定する工程、
(工程2)本発明の製造方法により製造された網膜組織又は網膜細胞を、生存可能な培養条件で、一定時間、被検物質の非存在下又はポジティブコントロールの存在下で培養した後、細胞の傷害の程度を測定する工程、及び
(工程3)(工程1)及び(工程2)において測定した結果の差異に基づき、工程1における被検物質が有する毒性を評価する工程。
本発明は、本発明の製造方法により製造される網膜組織又は網膜細胞(例、視細胞、網膜前駆細胞、網膜層特異的神経細胞)の有効量を含む医薬組成物を提供する。
本発明の製造方法により製造される網膜組織又は網膜細胞(例、視細胞、網膜組織、網膜前駆細胞、網膜層特異的神経細胞)は、当該網膜組織又は網膜細胞の障害に基づく疾患の移植医療に有用である。そこで、本発明は、本発明の製造方法により製造される網膜組織又は網膜細胞(例、視細胞、網膜前駆細胞、網膜層特異的神経細胞)を含む、当該網膜組織又は網膜細胞の障害に基づく疾患の治療薬を提供する。当該網膜組織又は網膜細胞の障害に基づく疾患の治療薬として、或いは、当該網膜組織の損傷状態において、該当する損傷部位を補充するために、本発明の製造方法により製造された網膜組織又は網膜細胞(網膜前駆細胞、網膜層特異的神経細胞を含む)を用いることが出来る。移植を必要とする、網膜組織又は網膜細胞の障害に基づく疾患、又は網膜組織の損傷状態の患者に、本発明の製造方法により製造された網膜組織又は網膜細胞を移植し、当該網膜細胞や、障害を受けた網膜組織自体を補充することにより、網膜組織又は網膜細胞の障害に基づく疾患、又は網膜組織の損傷状態を治療することが出来る。網膜組織又は網膜細胞の障害に基づく疾患としては、例えば、網膜変性症、網膜色素変性症、加齢黄斑変性症、有機水銀中毒、クロロキン網膜症、緑内障、糖尿病性網膜症、新生児網膜症などが挙げられる。
ヒトiPS細胞(1231A3株、京都大学より入手)を、Scientific Reports, 4, 3594 (2014)に記載の方法に準じてフィーダーフリー条件で培養した。フィーダーフリー培地としてはStemFit培地(AK03、味の素社製)、フィーダーフリー足場にはLaminin511-E8(ニッピ社製)を用いた。
ヒトiPS細胞(1231A3株、京都大学より入手)を、Scientific Reports, 4, 3594 (2014)に記載の方法に準じてフィーダーフリー条件で培養した。フィーダーフリー培地としてはStemFit培地(AK03、味の素社製)、フィーダーフリー足場にはLaminin511-E8(ニッピ社製)を用いた。維持培養操作は、比較例1記載の方法で行った。
ヒトiPS細胞(1231A3株、京都大学より入手)を、「Scientific Reports, 4, 3594 (2014)」に記載の方法に準じてフィーダーフリー条件で培養した。フィーダーフリー培地としてはEssential 8培地(Life Technologies社製)、フィーダーフリー足場にはLaminin511-E8(ニッピ社製)を用いた。
ヒトiPS細胞(201B7株、京都大学より入手)を、Scientific Reports, 4, 3594 (2014)に記載の方法に準じてフィーダーフリー培養した。フィーダーフリー培地としてはEssential 8培地(Life Technologies社製)、フィーダーフリー足場にはLaminin511-E8(ニッピ社製)を用いた。
ヒトiPS細胞(1231A3株、京都大学より入手)を、Scientific Reports, 4, 3594 (2014)に記載の方法に準じてフィーダーフリー培養した。フィーダーフリー培地としてはStemFit培地(AK03、味の素社製)、フィーダーフリー足場にはLaminin511-E8(ニッピ社製)を用いた。
条件1(+BMP、d3)としては、浮遊培養開始後3日目に、外来性のヒト組み換えBMP4の終濃度が1.5 nMになるように、Y27632及びSAGを含まず、ヒト組み換えBMP4(R&D社製)を含む培地を50μl加えた(培地量合計150μl)。浮遊培養開始後6日目に、Y27632、SAG及びヒト組み換えBMP4を含まない培地にて、半量培地交換した。
条件2(+BMP、d6)としては、浮遊培養開始後3日目に、Y27632、SAG及びヒト組み換えBMP4を含まない無血清培地を50μl加えた。浮遊培養開始後6日目に、Y27632及びSAGを含まず、ヒト組み換えBMP4(R&D社製)を含む培地にて、外来性のヒト組み換えBMP4の終濃度が1.5nMになるように半量培地交換した。
実施例4記載の方法で調製したフィーダーフリーヒトiPS細胞(1231A3株)を、TrypLE Select(Life Technologies社製)を用いて単一分散した後、前記単一分散されたヒトiPS細胞を非細胞接着性の96穴培養プレート(PrimeSurface 96V底プレート,住友ベークライト社)の1ウェルあたり1.2×104細胞になるように100μlの無血清培地に浮遊させ、37℃、5%CO2で浮遊培養した。その際の無血清培地(gfCDM+KSR)には、F-12培地とIMDM培地の1:1混合液に10%KSR、450μM 1-モノチオグリセロール、1 x Chemically defined lipid concentrateを添加した無血清培地を用いた。浮遊培養開始時(浮遊培養開始後0日目、工程2開始)に、前記無血清培地にY27632(終濃度20μM)及びSAG(終濃度600nM)を添加した。浮遊培養開始後2日目までに、細胞凝集体が形成された(工程2終了、工程3開始)。
条件1(+BMP、d3)としては、浮遊培養開始後3日目に、外来性のヒト組み換えBMP4の終濃度が1.5 nMになるように、Y27632及びSAGを含まず、ヒト組み換えBMP4(R&D社製)を含む培地を50μl加えた(培地量合計150μl)。浮遊培養開始後6日目に、Y27632、SAG及びヒト組み換えBMP4を含まない培地にて、半量培地交換した。
条件2(+BMP、d6)としては、浮遊培養開始後3日目に、Y27632、SAG及びヒト組み換えBMP4を含まない無血清培地を50μl加えた。浮遊培養開始後6日目に、外来性のヒト組み換えBMP4の終濃度が1.5 nMになるように、Y27632を含まず、ヒト組み換えBMP4(R&D社製)を含む培地にて、半量培地交換した。
ヒトiPS細胞(201B7株、京都大学より入手)を、Scientific Reports, 4, 3594 (2014)に記載の方法に準じてフィーダーフリー培養した。フィーダーフリー培地としてはStem Fit培地(AK-03;味の素社製)、フィーダーフリー足場にはLaminin511-E8(ニッピ社製)を用いた。
ヒトiPS細胞(1231A3株、京都大学より入手)を、Scientific Reports, 4, 3594 (2014)に記載の方法に準じてフィーダーフリー培養した。フィーダーフリー培地としてはStem Fit培地(AK-03;味の素社製)、フィーダーフリー足場にはLaminin511-E8(ニッピ社製)を用いた。
ヒトiPS細胞(DSPC-3株、大日本住友製薬にて樹立)は、市販されているセンダイウイルスベクター(Oct3/4、Sox2、KLF4、c-Mycの4因子、DNAVEC(現ID Pharma)社製サイトチューンキット)を用いて、Life Technologies社の公開プロトコル(iPS 2.0 Sendai Reprogramming Kit、Publication Number MAN0009378、Revision 1.0)、及び、京都大学の公開プロトコル(フィーダーフリーでのヒトiPS細胞の樹立・維持培養、CiRA_Ff-iPSC_protocol_JP_v140310, http://www.cira.kyoto-u.ac.jp/j/research/protocol.html)記載の方法をもとに、StemFit培地 (AK03;味の素社製)、Laminin511-E8(ニッピ社製)を用いて樹立した。
Claims (22)
- 下記工程(1)~(3)を含む、網膜細胞又は網膜組織の製造方法;
(1)ヒト多能性幹細胞を、フィーダー細胞非存在下で、未分化維持因子を含む培地で培養する第一工程、
(2)第一工程で得られた多能性幹細胞を、ソニック・ヘッジホッグシグナル伝達経路作用物質の存在下で浮遊培養し、細胞の凝集体を形成させる第二工程、及び
(3)第二工程で得られた凝集体を、BMPシグナル伝達経路作用物質の存在下に浮遊培養し、網膜細胞又は網膜組織を含む凝集体を得る第三工程。 - 第二工程において、第一工程で得られた細胞を分散し、当該分散した細胞を浮遊培養する、請求項1に記載の製造方法。
- 未分化維持因子が、FGFシグナル伝達経路作用物質である請求項1又は2に記載の製造方法。
- FGFシグナル伝達経路作用物質が、bFGFである、請求項3に記載の製造方法。
- 第二工程における培地中のソニック・ヘッジホッグシグナル伝達経路作用物質の濃度が、SAG10nM~700nMのソニック・ヘッジホッグシグナル伝達作用に相当する濃度である、請求項1~4のいずれかに記載の製造方法。
- ソニック・ヘッジホッグシグナル伝達経路作用物質がSAG、Purmorphamine又はShhである、請求項1~5のいずれかに記載の製造方法。
- BMPシグナル伝達経路作用物質が、BMP2、BMP4、BMP7及びGDF7からなる群から選ばれる1以上の蛋白質である、請求項1~6のいずれかに記載の製造方法。
- BMPシグナル伝達経路作用物質が、BMP4である、請求項6に記載の製造方法。
- 第三工程において、第二工程の開始後2日目から9日目の間にBMPシグナル伝達経路作用物質が培地に添加される、請求項1~8のいずれかに記載の製造方法。
- 第三工程において、ソニック・ヘッジホッグシグナル伝達経路作用物質の濃度がSAG700nMのソニック・ヘッジホッグシグナル伝達作用に相当する濃度以下である培地で凝集体を培養する、請求項1~9のいずれかに記載の製造方法。
- 第一工程が接着培養法で行われる、請求項1~10のいずれかに記載の製造方法。
- 多能性幹細胞が人工多能性幹細胞である、請求項1~11のいずれかに記載の製造方法。
- 第二工程において、均一な凝集体を形成させる、請求項1~12のいずれかに記載の製造方法。
- 第三工程において得られる凝集体が、網膜前駆細胞、神経網膜前駆細胞、視細胞前駆細胞、視細胞、桿体視細胞、錐体視細胞、水平細胞、アマクリン細胞、介在神経細胞、神経節細胞、網膜色素上皮細胞、及び毛様体周縁部細胞からなる群から選択される1又は複数の細胞を含む凝集体である、請求項1~13のいずれかに記載の製造方法。
- 浮遊培養が、基底膜標品非存在下で行われる請求項1~14のいずれかに記載の製造方法。
- 請求項1~15のいずれかに記載の方法により製造される網膜細胞又は網膜組織を含有してなる、被検物質の毒性・薬効評価用試薬。
- 請求項1~15のいずれかに記載の方法により製造される網膜細胞または網膜組織に被検物質を接触させ、該物質が該細胞又は該組織に及ぼす影響を検定することを含む、該物質の毒性・薬効評価方法。
- 請求項1~15のいずれかに記載の方法により製造される網膜細胞又は網膜組織を含有してなる、網膜組織の障害に基づく疾患の治療薬。
- 網膜細胞が網膜前駆細胞及び又は網膜層特異的神経細胞である、請求項18に記載の治療薬。
- 請求項1~15のいずれかに記載の方法により製造される、網膜細胞又は網膜組織の有効量を、移植を必要とする対象に移植することを含む、網膜組織の障害に基づく疾患の治療方法。
- 網膜組織の障害に基づく疾患の治療における使用のための請求項1~15のいずれかに記載の方法により製造される網膜細胞又は網膜組織。
- 請求項1~15のいずれかに記載の方法により製造される網膜細胞又は網膜組織を有効成分として含有する、医薬組成物。
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JP2016555415A JP6682446B2 (ja) | 2014-10-24 | 2015-10-23 | 網膜組織の製造方法 |
ES15852025T ES2880353T3 (es) | 2014-10-24 | 2015-10-23 | Método de producción de tejido retiniano |
DK15852025.4T DK3211071T3 (da) | 2014-10-24 | 2015-10-23 | Fremstillingsfremgangsmåde til retinalt væv |
SG11201703334VA SG11201703334VA (en) | 2014-10-24 | 2015-10-23 | Production method for retinal tissue |
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CN201580068968.0A CN107002040B (zh) | 2014-10-24 | 2015-10-23 | 视网膜组织的制备方法 |
IL251856A IL251856B (en) | 2014-10-24 | 2017-04-23 | A method for producing retinal tissue |
US17/567,708 US20220119764A1 (en) | 2014-10-24 | 2022-01-03 | Production method for retinal tissue |
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US20170313981A1 (en) | 2017-11-02 |
AU2015336454A1 (en) | 2017-05-25 |
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MY183058A (en) | 2021-02-09 |
KR20170072941A (ko) | 2017-06-27 |
DK3211071T3 (da) | 2021-07-26 |
ES2880353T3 (es) | 2021-11-24 |
TWI694150B (zh) | 2020-05-21 |
JPWO2016063986A1 (ja) | 2017-08-03 |
TW201629213A (zh) | 2016-08-16 |
JP7088496B2 (ja) | 2022-06-21 |
CN113528443B (zh) | 2023-11-03 |
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EP3211071A1 (en) | 2017-08-30 |
IL251856A0 (en) | 2017-06-29 |
KR102511192B1 (ko) | 2023-03-17 |
US20220119764A1 (en) | 2022-04-21 |
CN113528443A (zh) | 2021-10-22 |
EP3211071A4 (en) | 2018-05-30 |
JP2020114236A (ja) | 2020-07-30 |
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AU2015336454B2 (en) | 2021-04-29 |
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