WO2009041626A1 - 試料分析用試薬キット及び試料分析方法 - Google Patents

試料分析用試薬キット及び試料分析方法 Download PDF

Info

Publication number
WO2009041626A1
WO2009041626A1 PCT/JP2008/067507 JP2008067507W WO2009041626A1 WO 2009041626 A1 WO2009041626 A1 WO 2009041626A1 JP 2008067507 W JP2008067507 W JP 2008067507W WO 2009041626 A1 WO2009041626 A1 WO 2009041626A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample analysis
reagent kit
analysis method
reagent
disclosed
Prior art date
Application number
PCT/JP2008/067507
Other languages
English (en)
French (fr)
Inventor
Tatsuya Narikawa
Tomohiro Tsuji
Keiko Moriyama
Yuji Itose
Shinichiro Oguni
Ayumu Yoshida
Saori Suzuki
Toshihiro Mizukami
Original Assignee
Sysmex Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP08834533.5A priority Critical patent/EP2202516B1/en
Priority to JP2009534424A priority patent/JP5422390B2/ja
Priority to MX2010003125A priority patent/MX2010003125A/es
Priority to ES08834533.5T priority patent/ES2490865T3/es
Priority to BRPI0816016A priority patent/BRPI0816016B8/pt
Priority to KR1020107000283A priority patent/KR101244501B1/ko
Application filed by Sysmex Corporation filed Critical Sysmex Corporation
Priority to PL08834533T priority patent/PL2202516T3/pl
Priority to AU2008304761A priority patent/AU2008304761B2/en
Priority to US12/677,250 priority patent/US8802025B2/en
Priority to CN2008800247581A priority patent/CN101743469B/zh
Publication of WO2009041626A1 publication Critical patent/WO2009041626A1/ja
Priority to ZA2010/01253A priority patent/ZA201001253B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　本発明は、カチオン性界面活性剤、ノニオン性界面活性剤及び芳香族カルボン酸を含有する第１試薬と、核酸を染色可能な蛍光色素を含有する第２試薬とを含む試料分析用試薬キット、及び該キットを用いる試料分析方法を提供する。
PCT/JP2008/067507 2007-09-27 2008-09-26 試料分析用試薬キット及び試料分析方法 WO2009041626A1 (ja)

Priority Applications (11)

Application Number Priority Date Filing Date Title
JP2009534424A JP5422390B2 (ja) 2007-09-27 2008-09-26 試料分析用試薬キット及び試料分析方法
MX2010003125A MX2010003125A (es) 2007-09-27 2008-09-26 Equipo de reactivos para analisis de muestra y metodo de analisis de muestra.
ES08834533.5T ES2490865T3 (es) 2007-09-27 2008-09-26 Kit de reactivos para análisis de muestras y método de análisis de muestras
BRPI0816016A BRPI0816016B8 (pt) 2007-09-27 2008-09-26 kit reagente para analisar uma amostra e método para analisar uma amostra
KR1020107000283A KR101244501B1 (ko) 2007-09-27 2008-09-26 시료 분석용 시약 키트 및 시료 분석 방법
EP08834533.5A EP2202516B1 (en) 2007-09-27 2008-09-26 Reagent kit for sample analysis and sample analysis method
PL08834533T PL2202516T3 (pl) 2007-09-27 2008-09-26 Zestaw odczynników do analizy próbek i sposób analizy próbek
AU2008304761A AU2008304761B2 (en) 2007-09-27 2008-09-26 Reagent kit for sample analysis and sample analysis method
US12/677,250 US8802025B2 (en) 2007-09-27 2008-09-26 Reagent kit for sample analysis and sample analysis method
CN2008800247581A CN101743469B (zh) 2007-09-27 2008-09-26 试样分析用试剂盒以及试样分析方法
ZA2010/01253A ZA201001253B (en) 2007-09-27 2010-02-22 Reagent kit for sample analysis and sample analysis method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-252666 2007-09-27
JP2007252666 2007-09-27
JP2008-076606 2008-03-24
JP2008076606 2008-03-24

Publications (1)

Publication Number Publication Date
WO2009041626A1 true WO2009041626A1 (ja) 2009-04-02

Family

ID=40511506

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/067507 WO2009041626A1 (ja) 2007-09-27 2008-09-26 試料分析用試薬キット及び試料分析方法

Country Status (15)

Country Link
US (1) US8802025B2 (ja)
EP (1) EP2202516B1 (ja)
JP (2) JP5422390B2 (ja)
KR (1) KR101244501B1 (ja)
CN (1) CN101743469B (ja)
AU (1) AU2008304761B2 (ja)
BR (1) BRPI0816016B8 (ja)
CO (1) CO6270171A2 (ja)
ES (1) ES2490865T3 (ja)
MX (1) MX2010003125A (ja)
MY (1) MY150548A (ja)
PL (1) PL2202516T3 (ja)
RU (1) RU2461004C2 (ja)
WO (1) WO2009041626A1 (ja)
ZA (1) ZA201001253B (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009236798A (ja) * 2008-03-28 2009-10-15 Sysmex Corp 好塩基球の分類計数方法
JP2014520253A (ja) * 2011-05-04 2014-08-21 アボット・ラボラトリーズ 好塩基球分析システムおよび方法

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5583629B2 (ja) * 2011-04-28 2014-09-03 シスメックス株式会社 白血球の分類計数方法、白血球分類試薬キット及び白血球分類試薬
JP5865009B2 (ja) * 2011-10-25 2016-02-17 シスメックス株式会社 活性化好中球の検出方法
CN104297497B (zh) * 2013-07-16 2016-02-24 成都深迈瑞医疗电子技术研究院有限公司 血液分析方法、控制装置和血液细胞分析仪
WO2016106688A1 (zh) * 2014-12-31 2016-07-07 深圳迈瑞生物医疗电子股份有限公司 一种有核红细胞报警方法、装置及流式细胞分析仪
JP6710512B2 (ja) 2015-09-14 2020-06-17 シスメックス株式会社 血液分析装置、血液分析方法及びコンピュータプログラム
JP6789109B2 (ja) 2016-12-28 2020-11-25 富士フイルム株式会社 血液分析方法及び血液検査キット
CN112945667B (zh) * 2021-02-01 2024-03-26 海南师范大学 一种去除有核红细胞的血液单细胞悬液的制备方法
BR102023003038A2 (pt) 2022-03-17 2024-01-02 Sysmex Corporation Método para classificar glóbulos brancos em subpopulações

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6188896A (ja) 1984-09-24 1986-05-07 テクニコン、インストルメンツ、コーポレーシヨン 白血球弁別用組成物および弁別方法
JPH05149863A (ja) 1991-11-27 1993-06-15 Toa Medical Electronics Co Ltd 粒子計数方法
JPH06222059A (ja) * 1993-01-28 1994-08-12 Ibiden Co Ltd 蛍光標識試薬および蛍光免疫測定法
JPH07294518A (ja) 1994-04-21 1995-11-10 Toa Medical Electronics Co Ltd 白血球分析用試薬及び白血球の分類方法
JPH10339729A (ja) 1997-06-10 1998-12-22 Toa Medical Electronics Co Ltd 赤芽球の分類計数用試薬及び分類計数方法
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
JP2002148261A (ja) 2000-11-09 2002-05-22 Sysmex Corp 異常細胞分類計数方法
JP2006105625A (ja) * 2004-09-30 2006-04-20 Sysmex Corp 検体中粒子分析方法、分析装置及び分析用試薬

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3048260B2 (ja) * 1991-07-29 2000-06-05 シスメックス株式会社 白血球分類計数用試料調製方法
JP3067849B2 (ja) 1991-07-29 2000-07-24 シスメックス株式会社 白血球分類計数用試料調製方法
JP3320869B2 (ja) * 1993-12-22 2002-09-03 シスメックス株式会社 白血球分析用試薬
US5631165A (en) * 1994-08-01 1997-05-20 Abbott Laboratories Method for performing automated hematology and cytometry analysis
JP3355038B2 (ja) * 1994-08-03 2002-12-09 シスメックス株式会社 白血球分類方法
US6004536A (en) * 1995-11-14 1999-12-21 Molecular Probes, Inc. Lipophilic cyanine dyes with enchanced aqueous solubilty
JP3783808B2 (ja) 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
US6911313B2 (en) 1998-02-06 2005-06-28 Sysmex Corporation Process for discriminating and counting erythroblasts
JP4566299B2 (ja) * 1998-04-09 2010-10-20 シスメックス株式会社 赤芽球の分類計数方法
JP4212827B2 (ja) * 2002-05-16 2009-01-21 シスメックス株式会社 骨髄液有核細胞自動分析方法
JP4796443B2 (ja) * 2006-06-08 2011-10-19 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法
JP4914656B2 (ja) 2006-06-26 2012-04-11 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6188896A (ja) 1984-09-24 1986-05-07 テクニコン、インストルメンツ、コーポレーシヨン 白血球弁別用組成物および弁別方法
JPH05149863A (ja) 1991-11-27 1993-06-15 Toa Medical Electronics Co Ltd 粒子計数方法
JPH06222059A (ja) * 1993-01-28 1994-08-12 Ibiden Co Ltd 蛍光標識試薬および蛍光免疫測定法
JPH07294518A (ja) 1994-04-21 1995-11-10 Toa Medical Electronics Co Ltd 白血球分析用試薬及び白血球の分類方法
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
JPH10339729A (ja) 1997-06-10 1998-12-22 Toa Medical Electronics Co Ltd 赤芽球の分類計数用試薬及び分類計数方法
JP2002148261A (ja) 2000-11-09 2002-05-22 Sysmex Corp 異常細胞分類計数方法
JP2006105625A (ja) * 2004-09-30 2006-04-20 Sysmex Corp 検体中粒子分析方法、分析装置及び分析用試薬

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2202516A4

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009236798A (ja) * 2008-03-28 2009-10-15 Sysmex Corp 好塩基球の分類計数方法
JP2014520253A (ja) * 2011-05-04 2014-08-21 アボット・ラボラトリーズ 好塩基球分析システムおよび方法

Also Published As

Publication number Publication date
US20100330565A1 (en) 2010-12-30
ZA201001253B (en) 2011-04-28
CO6270171A2 (es) 2011-04-20
EP2202516A1 (en) 2010-06-30
KR101244501B1 (ko) 2013-03-18
BRPI0816016B1 (pt) 2020-10-13
MY150548A (en) 2014-01-30
JP5422390B2 (ja) 2014-02-19
AU2008304761B2 (en) 2011-08-04
KR20100055382A (ko) 2010-05-26
BRPI0816016A2 (pt) 2018-12-04
RU2461004C2 (ru) 2012-09-10
JP2013079968A (ja) 2013-05-02
PL2202516T3 (pl) 2015-02-27
CN101743469A (zh) 2010-06-16
JPWO2009041626A1 (ja) 2011-01-27
MX2010003125A (es) 2010-07-05
ES2490865T3 (es) 2014-09-04
AU2008304761A1 (en) 2009-04-02
US8802025B2 (en) 2014-08-12
BRPI0816016B8 (pt) 2021-07-27
EP2202516A4 (en) 2011-02-23
CN101743469B (zh) 2013-05-29
RU2010116388A (ru) 2011-11-10
EP2202516B1 (en) 2014-07-30
JP5600726B2 (ja) 2014-10-01

Similar Documents

Publication Publication Date Title
WO2009041626A1 (ja) 試料分析用試薬キット及び試料分析方法
WO2005071110A3 (en) Improving polynucleotide ligation reactions
WO2007098427A3 (en) Massively multiplexed sequencing
WO2006078289A3 (en) Bio-barcode based detection of target analytes
WO2008105896A3 (en) Analyte determination utilizing mass tagging reagents comprising a non-encoded detectable label
WO2007093050A8 (en) Gene expression assays conducted by elemental analysis
WO2009100197A3 (en) System for conducting the identification of bacteria in biological samples
EP2987866A3 (en) Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
WO2007140696A8 (fr) Kit elisa pour détecter le rouge soudan et procédé correspondant
BRPI0913107A2 (pt) Reagente de lise, ligação e/ou lavagem, uso do mesmo, método e kit para isolamento e/ou purificação de ácidos nucleicos de uma amostra biológica contendo ácidos nucleicos, e, uso de tensoativos não-iônicos com base em polixietileno.
WO2012061451A3 (en) Optical cup
WO2009121032A3 (en) Sample preparation devices and methods for processing analytes
WO2008133729A3 (en) Sequential analysis of biological samples with intermediate bleaching of fluorescence detector
WO2007100506A3 (en) Mass tags for quantitative analyses
WO2005106036A3 (en) Methods and compositions for assaying analytes
WO2006044994A3 (en) Primers, probes and methods for nucleic acid amplification
WO2007114986A3 (en) Cooperative probes and methods of using them
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
PL2082226T3 (pl) Sekwencyjna analiza próbek biologicznych
WO2009058902A3 (en) Methods and devices for analyte detection
WO2004092708A3 (en) Methods for the electrochemical detection of target compounds
WO2006069023A3 (en) Assay method and apparatus with reduced sample matrix effects
WO2005018418A3 (en) Automated quality control protocols in a multi-analyser system
EP2306204A4 (en) Nucleic acid analyzer, automatic analyzer, and analysis method
WO2007086900A3 (en) Stilbazium research assays

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200880024758.1

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08834533

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 20107000283

Country of ref document: KR

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 12010500052

Country of ref document: PH

ENP Entry into the national phase

Ref document number: 2009534424

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2008304761

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: PI 2010000548

Country of ref document: MY

Ref document number: 12677250

Country of ref document: US

Ref document number: 10027823

Country of ref document: CO

ENP Entry into the national phase

Ref document number: 2008304761

Country of ref document: AU

Date of ref document: 20080926

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: MX/A/2010/003125

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 1092/KOLNP/2010

Country of ref document: IN

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2010116388

Country of ref document: RU

REG Reference to national code

Ref country code: BR

Ref legal event code: B01E

Ref document number: PI0816016

Country of ref document: BR

ENP Entry into the national phase

Ref document number: PI0816016

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20100324