RU2004114230A - Кофейное растение с пониженной активностью a-d-галактозидазы - Google Patents
Кофейное растение с пониженной активностью a-d-галактозидазы Download PDFInfo
- Publication number
- RU2004114230A RU2004114230A RU2004114230/13A RU2004114230A RU2004114230A RU 2004114230 A RU2004114230 A RU 2004114230A RU 2004114230/13 A RU2004114230/13 A RU 2004114230/13A RU 2004114230 A RU2004114230 A RU 2004114230A RU 2004114230 A RU2004114230 A RU 2004114230A
- Authority
- RU
- Russia
- Prior art keywords
- coffee
- plant
- galactosidase activity
- plant cell
- coffee plant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Tea And Coffee (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Claims (8)
1. Клетка кофейного растения, продуцирующая галактоманнаны, которая модифицирована таким образом, что эндогенный уровень активности-галактозидазы снижен так, что уровень разветвления галактозы в галактоманнанах повышен.
2. Клетка кофейного растения по п.1, включающая нуклеиновую кислоту, которая транскрибирована в рибонуклеиновую кислоту, которая является антисмысловой к мРНК, или ее часть, происходящая из гена-D-галактозидазы.
3. Клетка кофейного растения по п.2, отличающаяся тем, что нуклеиновая кислота, которая транскрибирована в рибонуклеиновую кислоту, которая является антисмысловой к мРНК, или ее часть, происходящая из гена-D-галактозидазы, находится под контролем конститутивного или индуцибельного промотора.
4. Клетка кофейного растения по п.3, отличающаяся тем, что промотор представляет собой кофейный промотор cspl.
5. Кофейное растение, содержащее растительную клетку по любому из предшествующих пунктов.
6. Способ получения растворимого кофе, включающий стадию использования кофейных зерен, полученных из кофейного растения по п.5.
7. Способ повышения растворимости кофе, включающий стадию использования кофейных зерен, полученных из растения по п.5.
8. Применение зерен, полученных из кофейного растения по п.5, для получения растворимого кофе.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01124160.1 | 2001-10-10 | ||
EP01124160 | 2001-10-10 |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2004114230A true RU2004114230A (ru) | 2005-04-20 |
RU2303349C2 RU2303349C2 (ru) | 2007-07-27 |
Family
ID=8178911
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2004114230/13A RU2303349C2 (ru) | 2001-10-10 | 2002-08-15 | КОФЕЙНОЕ РАСТЕНИЕ С ПОНИЖЕННОЙ АКТИВНОСТЬЮ α-D-ГАЛАКТОЗИДАЗЫ |
Country Status (16)
Country | Link |
---|---|
US (1) | US7238858B2 (ru) |
EP (1) | EP1436402B1 (ru) |
JP (1) | JP4276945B2 (ru) |
CN (1) | CN1635895A (ru) |
AT (1) | ATE318319T1 (ru) |
AU (1) | AU2002333434B2 (ru) |
BR (1) | BR0213180A (ru) |
CA (1) | CA2457821A1 (ru) |
CO (1) | CO5580845A2 (ru) |
DE (1) | DE60209380T2 (ru) |
ES (1) | ES2258173T3 (ru) |
MY (1) | MY129821A (ru) |
OA (1) | OA12667A (ru) |
RU (1) | RU2303349C2 (ru) |
WO (1) | WO2003032713A2 (ru) |
ZA (1) | ZA200403452B (ru) |
Families Citing this family (53)
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ES2600781T3 (es) | 2008-12-04 | 2017-02-10 | Curna, Inc. | Tratamiento para enfermedades relacionadas con el factor de crecimiento del endotelio vascular (vegf) mediante la inhibición de transcritos antisentido naturales de vegf |
KR101829469B1 (ko) | 2008-12-04 | 2018-03-30 | 큐알엔에이, 인크. | Epo에 대한 천연 안티센스 전사체의 억제에 의해 에리트로포에틴(epo) 관련된 질환의 치료 |
WO2010093904A2 (en) | 2009-02-12 | 2010-08-19 | Curna, Inc. | Treatment of brain derived neurotrophic factor (bdnf) related diseases by inhibition of natural antisense transcript to bdnf |
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KR101807323B1 (ko) | 2009-06-24 | 2017-12-08 | 큐알엔에이, 인크. | Tnfr2에 대한 천연 안티센스 전사체의 억제에 의한 종양 괴사 인자 수용체 2(tnfr2) 관련된 질환의 치료 |
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US10000752B2 (en) | 2010-11-18 | 2018-06-19 | Curna, Inc. | Antagonat compositions and methods of use |
KR102010598B1 (ko) | 2010-11-23 | 2019-08-13 | 큐알엔에이, 인크. | Nanog에 대한 자연 안티센스 전사체의 저해에 의한 nanog 관련된 질환의 치료 |
RU2620980C2 (ru) | 2011-06-09 | 2017-05-30 | Курна, Инк. | Лечение заболеваний, связанных с фратаксином (fxn), путем ингибирования природного антисмыслового транскрипта fxn |
EA029151B1 (ru) | 2011-09-06 | 2018-02-28 | Курна, Инк. | ЛЕЧЕНИЕ ЗАБОЛЕВАНИЙ, СВЯЗАННЫХ С АЛЬФА-СУБЪЕДИНИЦАМИ ПОТЕНЦИАЛЗАВИСИМЫХ НАТРИЕВЫХ КАНАЛОВ (SCNxA), С ПОМОЩЬЮ МАЛЫХ МОЛЕКУЛ |
WO2013138374A2 (en) | 2012-03-15 | 2013-09-19 | Curna, Inc. | Treatment of brain derived neurotrophic factor (bdnf) related diseases by inhibition of natural antisense transcript to bdnf |
CN102643800B (zh) * | 2012-04-25 | 2014-02-05 | 中国科学院植物研究所 | 一种提取植物dna的方法及其专用试剂盒 |
GB201708665D0 (en) * | 2017-05-31 | 2017-07-12 | Tropic Biosciences Uk Ltd | Compositions and methods for increasing extractability of solids from coffee beans |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6329191B1 (en) * | 1993-08-30 | 2001-12-11 | Hawaii Biotechnology Group, Inc. | DNA encoding recombinant coffee bean alpha-galactosidase |
EP1138771A1 (fr) * | 2000-03-30 | 2001-10-04 | Societe Des Produits Nestle S.A. | Endo-Mannanase de café |
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2002
- 2002-08-15 DE DE60209380T patent/DE60209380T2/de not_active Expired - Lifetime
- 2002-08-15 CN CNA028200861A patent/CN1635895A/zh active Pending
- 2002-08-15 AU AU2002333434A patent/AU2002333434B2/en not_active Ceased
- 2002-08-15 RU RU2004114230/13A patent/RU2303349C2/ru not_active IP Right Cessation
- 2002-08-15 EP EP02801285A patent/EP1436402B1/en not_active Expired - Lifetime
- 2002-08-15 BR BR0213180-3A patent/BR0213180A/pt not_active IP Right Cessation
- 2002-08-15 ES ES02801285T patent/ES2258173T3/es not_active Expired - Lifetime
- 2002-08-15 WO PCT/EP2002/009148 patent/WO2003032713A2/en active IP Right Grant
- 2002-08-15 AT AT02801285T patent/ATE318319T1/de not_active IP Right Cessation
- 2002-08-15 JP JP2003535528A patent/JP4276945B2/ja not_active Expired - Fee Related
- 2002-08-15 CA CA002457821A patent/CA2457821A1/en not_active Abandoned
- 2002-08-15 OA OA1200400102A patent/OA12667A/en unknown
- 2002-08-26 MY MYPI20023142A patent/MY129821A/en unknown
-
2004
- 2004-03-19 US US10/804,096 patent/US7238858B2/en not_active Expired - Lifetime
- 2004-04-05 CO CO04032188A patent/CO5580845A2/es not_active Application Discontinuation
- 2004-05-06 ZA ZA200403452A patent/ZA200403452B/en unknown
Also Published As
Publication number | Publication date |
---|---|
DE60209380T2 (de) | 2006-10-19 |
RU2303349C2 (ru) | 2007-07-27 |
US7238858B2 (en) | 2007-07-03 |
JP2005505290A (ja) | 2005-02-24 |
WO2003032713A2 (en) | 2003-04-24 |
ZA200403452B (en) | 2006-07-26 |
CA2457821A1 (en) | 2003-04-24 |
EP1436402A2 (en) | 2004-07-14 |
EP1436402B1 (en) | 2006-02-22 |
AU2002333434B2 (en) | 2007-12-13 |
BR0213180A (pt) | 2004-09-14 |
US20040199943A1 (en) | 2004-10-07 |
OA12667A (en) | 2006-06-19 |
WO2003032713A3 (en) | 2003-11-13 |
ATE318319T1 (de) | 2006-03-15 |
CO5580845A2 (es) | 2005-11-30 |
JP4276945B2 (ja) | 2009-06-10 |
CN1635895A (zh) | 2005-07-06 |
ES2258173T3 (es) | 2006-08-16 |
MY129821A (en) | 2007-05-31 |
DE60209380D1 (de) | 2006-04-27 |
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