RU2004114230A - Кофейное растение с пониженной активностью a-d-галактозидазы - Google Patents

Кофейное растение с пониженной активностью a-d-галактозидазы Download PDF

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Publication number
RU2004114230A
RU2004114230A RU2004114230/13A RU2004114230A RU2004114230A RU 2004114230 A RU2004114230 A RU 2004114230A RU 2004114230/13 A RU2004114230/13 A RU 2004114230/13A RU 2004114230 A RU2004114230 A RU 2004114230A RU 2004114230 A RU2004114230 A RU 2004114230A
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Russia
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coffee
plant
galactosidase activity
plant cell
coffee plant
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RU2004114230/13A
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English (en)
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RU2303349C2 (ru
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Пьер МАРРАЧЧИНИ (BR)
Пьер МАРРАЧЧИНИ
Ален ДЕЗЕЙ (FR)
Ален ДЕЗЕЙ
Джон РОДЖЕРС (FR)
Джон Роджерс
Original Assignee
Сосьете де Продюи Нестле С.А. (CH)
Сосьете Де Продюи Нестле С.А.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Tea And Coffee (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Claims (8)

1. Клетка кофейного растения, продуцирующая галактоманнаны, которая модифицирована таким образом, что эндогенный уровень активности-галактозидазы снижен так, что уровень разветвления галактозы в галактоманнанах повышен.
2. Клетка кофейного растения по п.1, включающая нуклеиновую кислоту, которая транскрибирована в рибонуклеиновую кислоту, которая является антисмысловой к мРНК, или ее часть, происходящая из гена-D-галактозидазы.
3. Клетка кофейного растения по п.2, отличающаяся тем, что нуклеиновая кислота, которая транскрибирована в рибонуклеиновую кислоту, которая является антисмысловой к мРНК, или ее часть, происходящая из гена-D-галактозидазы, находится под контролем конститутивного или индуцибельного промотора.
4. Клетка кофейного растения по п.3, отличающаяся тем, что промотор представляет собой кофейный промотор cspl.
5. Кофейное растение, содержащее растительную клетку по любому из предшествующих пунктов.
6. Способ получения растворимого кофе, включающий стадию использования кофейных зерен, полученных из кофейного растения по п.5.
7. Способ повышения растворимости кофе, включающий стадию использования кофейных зерен, полученных из растения по п.5.
8. Применение зерен, полученных из кофейного растения по п.5, для получения растворимого кофе.
RU2004114230/13A 2001-10-10 2002-08-15 КОФЕЙНОЕ РАСТЕНИЕ С ПОНИЖЕННОЙ АКТИВНОСТЬЮ α-D-ГАЛАКТОЗИДАЗЫ RU2303349C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01124160.1 2001-10-10
EP01124160 2001-10-10

Publications (2)

Publication Number Publication Date
RU2004114230A true RU2004114230A (ru) 2005-04-20
RU2303349C2 RU2303349C2 (ru) 2007-07-27

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Country Status (16)

Country Link
US (1) US7238858B2 (ru)
EP (1) EP1436402B1 (ru)
JP (1) JP4276945B2 (ru)
CN (1) CN1635895A (ru)
AT (1) ATE318319T1 (ru)
AU (1) AU2002333434B2 (ru)
BR (1) BR0213180A (ru)
CA (1) CA2457821A1 (ru)
CO (1) CO5580845A2 (ru)
DE (1) DE60209380T2 (ru)
ES (1) ES2258173T3 (ru)
MY (1) MY129821A (ru)
OA (1) OA12667A (ru)
RU (1) RU2303349C2 (ru)
WO (1) WO2003032713A2 (ru)
ZA (1) ZA200403452B (ru)

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DE60209380T2 (de) 2006-10-19
RU2303349C2 (ru) 2007-07-27
US7238858B2 (en) 2007-07-03
JP2005505290A (ja) 2005-02-24
WO2003032713A2 (en) 2003-04-24
ZA200403452B (en) 2006-07-26
CA2457821A1 (en) 2003-04-24
EP1436402A2 (en) 2004-07-14
EP1436402B1 (en) 2006-02-22
AU2002333434B2 (en) 2007-12-13
BR0213180A (pt) 2004-09-14
US20040199943A1 (en) 2004-10-07
OA12667A (en) 2006-06-19
WO2003032713A3 (en) 2003-11-13
ATE318319T1 (de) 2006-03-15
CO5580845A2 (es) 2005-11-30
JP4276945B2 (ja) 2009-06-10
CN1635895A (zh) 2005-07-06
ES2258173T3 (es) 2006-08-16
MY129821A (en) 2007-05-31
DE60209380D1 (de) 2006-04-27

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