MX361154B - Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso. - Google Patents

Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso.

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Publication number
MX361154B
MX361154B MX2016002266A MX2016002266A MX361154B MX 361154 B MX361154 B MX 361154B MX 2016002266 A MX2016002266 A MX 2016002266A MX 2016002266 A MX2016002266 A MX 2016002266A MX 361154 B MX361154 B MX 361154B
Authority
MX
Mexico
Prior art keywords
methods
genome
cas endonuclease
cell
guide polynucleotide
Prior art date
Application number
MX2016002266A
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English (en)
Other versions
MX2016002266A (es
Inventor
K Young Joshua
A Patten Phillip
Cigan Andrew
Original Assignee
Pioneer Hi Bred Int
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Publication date
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Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of MX2016002266A publication Critical patent/MX2016002266A/es
Publication of MX361154B publication Critical patent/MX361154B/es

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    • A—HUMAN NECESSITIES
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    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
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  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
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  • Nutrition Science (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physiology (AREA)
  • Immunology (AREA)
  • Insects & Arthropods (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

Se proporciona composiciones y métodos para la modificación genómica de una secuencia objetivo en el genoma de una célula. Los métodos y composiciones usan un sistema de polinucleótido guía/endonucleasa Cas para proporcionar un sistema eficaz para modificar o alterar sitios objetivo dentro del genoma de una célula u organismo. Una vez que se identificó un sitio genómico objetivo puede usarse diversos métodos para modificar, además, los sitios objetivo de manera que contengan diversos polinucleótidos de interés. Además, se proporciona composiciones y métodos para editar una secuencia de nucleótidos en el genoma de una célula. Además, se describen métodos de cultivo y métodos para seleccionar plantas con el uso de un sistema bicomponente de polinucleótido de ARN y endonucleasa CAS.
MX2016002266A 2013-08-22 2014-08-20 Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso. MX361154B (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201361868706P 2013-08-22 2013-08-22
US201361882532P 2013-09-25 2013-09-25
US201461937045P 2014-02-07 2014-02-07
US201461953090P 2014-03-14 2014-03-14
US201462023239P 2014-07-11 2014-07-11
PCT/US2014/051780 WO2015026885A1 (en) 2013-08-22 2014-08-20 Genome modification using guide polynucleotide/cas endonuclease systems and methods of use

Publications (2)

Publication Number Publication Date
MX2016002266A MX2016002266A (es) 2016-07-26
MX361154B true MX361154B (es) 2018-11-28

Family

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Family Applications (5)

Application Number Title Priority Date Filing Date
MX2016002266A MX361154B (es) 2013-08-22 2014-08-20 Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso.
MX2016002118A MX2016002118A (es) 2013-08-22 2014-08-20 Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
MX2016002306A MX2016002306A (es) 2013-08-22 2014-08-20 Promotor u6 de polimerasa iii de soja y metodos de uso.
MX2016002119A MX363842B (es) 2013-08-22 2014-08-20 Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.
MX2018014501A MX2018014501A (es) 2013-08-22 2016-02-19 Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso.

Family Applications After (4)

Application Number Title Priority Date Filing Date
MX2016002118A MX2016002118A (es) 2013-08-22 2014-08-20 Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
MX2016002306A MX2016002306A (es) 2013-08-22 2014-08-20 Promotor u6 de polimerasa iii de soja y metodos de uso.
MX2016002119A MX363842B (es) 2013-08-22 2014-08-20 Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.
MX2018014501A MX2018014501A (es) 2013-08-22 2016-02-19 Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso.

Country Status (12)

Country Link
US (16) US20150082478A1 (es)
EP (6) EP3036332B1 (es)
CN (4) CN105916989A (es)
AU (6) AU2014308896A1 (es)
BR (3) BR112016003591A8 (es)
CA (7) CA2922046A1 (es)
DK (1) DK3036327T3 (es)
ES (1) ES2729635T3 (es)
HU (1) HUE044916T2 (es)
LT (1) LT3036327T (es)
MX (5) MX361154B (es)
WO (4) WO2015026886A1 (es)

Families Citing this family (285)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2831144A1 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
CA2853829C (en) 2011-07-22 2023-09-26 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150225734A1 (en) 2012-06-19 2015-08-13 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
EP3372679A1 (en) * 2012-10-23 2018-09-12 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
SG11201508028QA (en) 2013-04-16 2015-10-29 Regeneron Pharma Targeted modification of rat genome
WO2014199358A1 (en) 2013-06-14 2014-12-18 Cellectis Methods for non-transgenic genome editing in plants
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
MX361154B (es) 2013-08-22 2018-11-28 Pioneer Hi Bred Int Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso.
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