MX344963B - Enzimas celulasas optimizadas. - Google Patents

Enzimas celulasas optimizadas.

Info

Publication number
MX344963B
MX344963B MX2012009357A MX2012009357A MX344963B MX 344963 B MX344963 B MX 344963B MX 2012009357 A MX2012009357 A MX 2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A MX 344963 B MX344963 B MX 344963B
Authority
MX
Mexico
Prior art keywords
enzymes
disclosed
cellulase enzymes
cellulose
processing
Prior art date
Application number
MX2012009357A
Other languages
English (en)
Other versions
MX2012009357A (es
Inventor
Ulrich Kettling
Andre Koltermann
Christoph Reisinger
Thomas Brück
Jochen Gerlach
Isabel Unterstrasser
Lutz RÖCHER
Markus Rarbach
Jörg Claren
Andreas Kohl
Jan Carsten Pieck
Dominik Schlosser
Original Assignee
Süd-Chemie IP GmbH & Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Süd-Chemie IP GmbH & Co KG filed Critical Süd-Chemie IP GmbH & Co KG
Publication of MX2012009357A publication Critical patent/MX2012009357A/es
Publication of MX344963B publication Critical patent/MX344963B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

La presente invención se refiere a enzimas celulasa con propiedades optimizadas para procesamiento de sustratos que contienen celulosa y lignocelulosa. En particular, se desvelan enzimas celobiohidrolasa con características preferidas. La presente invención proporciona variantes de fusión, inserción, deleción y/o sustitución de tales enzimas. Las variantes enzimáticas tienen termoestabilidad, estabilidad proteolítica, actividad específica y/o estabilidad potenciadas a pH extremo. Se desvelan moléculas de ácido nucleico que codifican dichas enzimas, una composición que comprende dichas enzimas, un procedimiento para preparación y el uso para procesamiento de celulosa y/o para la producción de biocarburantes.
MX2012009357A 2010-02-11 2011-02-11 Enzimas celulasas optimizadas. MX344963B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10153355.2A EP2357227B1 (en) 2010-02-11 2010-02-11 Optimized cellulase enzymes
PCT/EP2011/052023 WO2011098551A2 (en) 2010-02-11 2011-02-11 Optimized cellulase enzymes

Publications (2)

Publication Number Publication Date
MX2012009357A MX2012009357A (es) 2012-12-17
MX344963B true MX344963B (es) 2017-01-11

Family

ID=42027636

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012009357A MX344963B (es) 2010-02-11 2011-02-11 Enzimas celulasas optimizadas.

Country Status (14)

Country Link
US (1) US9458443B2 (es)
EP (2) EP2357227B1 (es)
CN (1) CN102844430A (es)
AU (1) AU2011214298B2 (es)
BR (1) BR112012020277A2 (es)
CA (1) CA2789423C (es)
DK (2) DK2357227T3 (es)
ES (2) ES2549328T3 (es)
HR (2) HRP20151043T1 (es)
MX (1) MX344963B (es)
PL (2) PL2357227T3 (es)
RS (1) RS57695B1 (es)
SI (2) SI2357227T1 (es)
WO (1) WO2011098551A2 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011153276A2 (en) 2010-06-01 2011-12-08 California Institute Of Technology Stable, functional chimeric cellobiohydrolase class i enzymes
US9096871B2 (en) * 2010-10-06 2015-08-04 Bp Corporation North America Inc. Variant CBH I polypeptides with reduced product inhibition
WO2012135719A1 (en) * 2011-03-31 2012-10-04 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
EP2780450A4 (en) * 2011-11-15 2015-09-09 Novozymes Inc POLYPEPTIDES HAVING CELLOBIOHYDROLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
DK2794664T3 (en) * 2011-12-22 2017-08-21 Novozymes Inc HYBRID POLYPEPTIDES WITH CELLOBIO HYDROLASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
CA2893059A1 (en) * 2012-12-12 2014-06-19 Danisco Us Inc. Variants of cellobiohydrolases
EP2931887A1 (en) 2012-12-12 2015-10-21 Danisco US Inc. Variants of cellobiohydrolases
EP2964760B1 (en) * 2013-03-08 2021-05-12 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
US11390898B2 (en) 2014-09-05 2022-07-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
DK3191587T3 (da) 2014-09-08 2019-10-14 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
JP6378622B2 (ja) * 2014-12-04 2018-08-22 本田技研工業株式会社 高活性セロビオヒドロラーゼ
WO2016138167A2 (en) 2015-02-24 2016-09-01 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
KR20190018512A (ko) 2016-06-15 2019-02-22 코덱시스, 인코포레이티드 조작된 베타-글루코시다아제 및 글루코실화 방법
CN109337888B (zh) * 2018-11-12 2021-08-17 南开大学 纤维二糖水解酶突变体及其生产方法和应用
CN110373402B (zh) * 2019-08-13 2021-07-20 山东农业大学 一种提升耐热纤维素酶酶活力和热稳定性的方法
CN113186175B (zh) * 2021-06-04 2023-08-04 翌圣生物科技(上海)股份有限公司 突变型Taq DNA聚合酶、编码DNA序列、重组载体、重组表达细胞及其应用
CN114426961B (zh) * 2022-03-10 2024-01-26 安徽大学 一种β-葡萄糖苷酶突变体、其编码基因、表达菌株及其应用
CN117802072B (zh) * 2024-02-29 2024-05-24 北京挑战生物技术有限公司 木聚糖酶突变体及其应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
US8637293B2 (en) 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
US7375197B2 (en) * 2002-01-14 2008-05-20 Midwest Research Institute Cellobiohydrolase I gene and improved variants
WO2001004284A1 (en) 1999-07-13 2001-01-18 Midwest Research Institute Cellobiohydrolase reduced glycosylation variants: cbhin45a; cbhin270a; and cbhin384a
EP2298868B1 (en) 2001-06-26 2015-01-07 Novozymes A/S Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same
DK1578943T3 (da) 2002-08-16 2012-01-09 Danisco Us Inc Nye variant-Hypocrea jecorina-CBH1-cellulaser
US7449550B2 (en) * 2003-02-27 2008-11-11 Alliance For Sustainable Energy, Llc Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
JP2007534294A (ja) 2003-03-21 2007-11-29 ジェネンコー・インターナショナル・インク Ｃｂｈ１相同体及び変異体ｃｂｈ１セルラーゼ
CA2771875A1 (en) 2003-04-01 2005-01-06 Danisco Us Inc. Variant hypocrea jecorina cbh1
CN103088001A (zh) 2003-08-25 2013-05-08 诺维信股份有限公司 糖苷水解酶的变体
PT1874927E (pt) * 2005-04-29 2014-04-23 Ab Enzymes Oy Celulases melhoradas
CN1757710A (zh) 2005-07-15 2006-04-12 山东农业大学 表达cbh1基因的酵母工程菌株及其构建方法
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
US8017373B2 (en) * 2006-08-31 2011-09-13 Iogen Energy Corporation Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks
US10676751B2 (en) 2008-02-29 2020-06-09 The Trustees Of The University Of Pennsylvania Production and use of plant degrading materials
US9994835B2 (en) * 2008-05-11 2018-06-12 Lallemand Hungary Liquidity Management Llc Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases
BRPI0911966B1 (pt) * 2008-05-11 2018-11-13 Univ Stellenbosch polinucleotídeo codificando uma celobioidrolase
CA2729924C (en) * 2008-07-07 2018-05-29 Mascoma Corporation Isolation and characterization of schizochytrium aggregatum cellobiohydrolase i (cbh 1)
WO2010122141A1 (en) * 2009-04-24 2010-10-28 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof

Also Published As

Publication number Publication date
ES2549328T3 (es) 2015-10-27
EP2357227B1 (en) 2015-07-15
DK2534245T3 (en) 2018-10-01
BR112012020277A2 (pt) 2015-09-15
RS57695B1 (sr) 2018-11-30
DK2357227T3 (en) 2015-09-14
EP2534245A2 (en) 2012-12-19
CA2789423C (en) 2017-12-12
SI2534245T1 (sl) 2019-01-31
SI2357227T1 (sl) 2015-11-30
WO2011098551A2 (en) 2011-08-18
WO2011098551A3 (en) 2012-05-31
HRP20181587T1 (hr) 2018-12-14
EP2534245B1 (en) 2018-07-04
US20130123115A1 (en) 2013-05-16
ES2689939T3 (es) 2018-11-16
US9458443B2 (en) 2016-10-04
CN102844430A (zh) 2012-12-26
PL2357227T3 (pl) 2015-12-31
HRP20151043T1 (hr) 2015-11-06
EP2357227A1 (en) 2011-08-17
PL2534245T3 (pl) 2019-02-28
AU2011214298A1 (en) 2012-09-06
CA2789423A1 (en) 2011-08-18
MX2012009357A (es) 2012-12-17
AU2011214298B2 (en) 2014-11-06

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