HRP20151043T1 - Optimizirani celulaza enzimi - Google Patents
Optimizirani celulaza enzimi Download PDFInfo
- Publication number
- HRP20151043T1 HRP20151043T1 HRP20151043TT HRP20151043T HRP20151043T1 HR P20151043 T1 HRP20151043 T1 HR P20151043T1 HR P20151043T T HRP20151043T T HR P20151043TT HR P20151043 T HRP20151043 T HR P20151043T HR P20151043 T1 HRP20151043 T1 HR P20151043T1
- Authority
- HR
- Croatia
- Prior art keywords
- polypeptide
- seq
- amino acid
- segment
- polypeptide according
- Prior art date
Links
- 108010059892 Cellulase Proteins 0.000 title claims 2
- 229920001184 polypeptide Polymers 0.000 claims 18
- 102000004196 processed proteins & peptides Human genes 0.000 claims 18
- 108090000765 processed proteins & peptides Proteins 0.000 claims 18
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 11
- 150000001413 amino acids Chemical class 0.000 claims 6
- 125000000539 amino acid group Chemical group 0.000 claims 5
- 241000235648 Pichia Species 0.000 claims 3
- 230000037430 deletion Effects 0.000 claims 3
- 238000012217 deletion Methods 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 238000002360 preparation method Methods 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 239000004615 ingredient Substances 0.000 claims 2
- 241000228212 Aspergillus Species 0.000 claims 1
- 239000002028 Biomass Substances 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- 241000235649 Kluyveromyces Species 0.000 claims 1
- 241000235070 Saccharomyces Species 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 241000235346 Schizosaccharomyces Species 0.000 claims 1
- 241000223259 Trichoderma Species 0.000 claims 1
- 108010047754 beta-Glucosidase Proteins 0.000 claims 1
- 102000006995 beta-Glucosidase Human genes 0.000 claims 1
- 239000003599 detergent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000007515 enzymatic degradation Effects 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 239000004753 textile Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Detergent Compositions (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (14)
1. Polipeptid koji ima aktivnost celobiohidrolaze, naznačen time, da polipeptid sadrži aminokiselinski odsječak s najmanje 85 % identiteta odsječka SEQ ID NO: 2, pri čemu je aminokiselinski ostatak na položaju Q1 od SEQ ID NO: 2 modificiran supstitucijom ili delecijom.
2. Polipeptid u skladu s patentnim zahtjevom 1, naznačen time, da polipeptid zadržava 50 % svoje maksimalne snage pretvorbe supstrata kada se pretvorba provodi u trajanju od 60 minuta na temperaturi od 60 °C ili višoj.
3. Polipeptid u skladu s patentnim zahtjevom 1 ili 2, naznačen time, da polipeptid obuhvaća aminokiselinski odsječak s najmanje 90 %, poželjno barem 95 %, a poželjnije je najmanje 99 % identičnosti odsječka sa SEQ ID NO: 2.
4. Polipeptid u skladu s jednim ili više prethodnih patentnih zahtjeva, naznačen time, da aminokiselinski odsječak polipeptida ima odsječak kao što je definirano sa SEQ ID NO: 2 ili odsječak kao što je definirano sa SEQ ID NO: 2, pri čemu je 1 do 75 amino kiselinskih ostataka, još bolje 1 do 35 aminokiselinskih ostataka supstituirano, deletirano ili umetnuto.
5. Polipeptid u skladu s patentnim zahtjevom 4, naznačen time, da su dodatno jedan ili više od sljedećih aminokiselinskih ostataka odsječka definiranog sa SEQ ID NO: 2 modificirani supstitucijom ili brisanjem: položaji G4, A6, T15, Q28, W40, D64, E65, A72, S86, K92, V130, V152, Y155, K159, D181, E183, N194, D202, P224, T243, Y244, I277, K304, N310, S311, N318, D320, T335, T344, D346, Q349, A358, Y374, A375, T392, T393, D410, Y422, P442, N445, R446, T456, S460, P462, G463, H468 i/ili V482 aminokiselina 1 do 500 iz SEQ ID NO: 2.
6. Polipeptid u skladu s patentnim zahtjevom 5, naznačen time, da polipeptid sadrži jedan ili više od slijedećih poželjnih razmjena s obzirom na odsječak definiran sa SEQ ID NO: 2:
[image]
7. Polipeptid u skladu s jednim ili više prethodnih patentnih zahtjeva, naznačen time, da polipeptid ima aminokiselinski odsječak odabran iz popisa sljedećih mutacija SEQ ID NO: 2:
[image]
8. Polipeptid u skladu s jednim ili više od prethodnih zahtjeva, koji se ekspremira i izlučuje u količini od više od 100 mg/l, još poželjnije više od 200 mg/l, posebno povoljno više od 500 mg/l i najpoželjnije više od 1 g/l u supernatantu nakon uvođenja nukleinske kiseline koja kodira polipeptid koji ima aminokiselinski odsječak s najmanje 85 % identičnosti sekvence sa SEQ ID NO: 2 u kvascu, naznačen time, da se aminokiselinski ostatak na položaju Q1 SEQ ID NO: 2 mora modificirati supstitucijom ili delecijom.
9. Nukleinska kiselina koja kodira polipeptid u skladu s jednim ili više patentnih zahtjeva od 1 do 8, naznačena time, da po mogućnosti ima najmanje 95 % identiteta od SEQ ID NO: 1.
10. Vektor, naznačen time, da sadrži nukleinsku kiselinu u skladu s patentnim zahtjevom 9.
11. Stanica domaćin, naznačena time, da je transformirana vektorom u skladu s patentnim zahtjevom 10.
12. Stanica-domaćin u skladu s patentnim zahtjevom 11, naznačena time, da je stanica domaćin izvedena iz skupine koja se sastoji od Saccharomyces, Schizosaccharomyces, Kluyveromyces, Pichia, Pichia, Hansenula, Aspergillus, Trichoderma, Penidllium, i Candida Yarrowina.
13. Pripravak, naznačen time, da sadržava polipeptid u skladu sa jednim ili više patentnih zahtjeva 1 do 9 i jednom ili više endoglukanazom i/ili jednom ili više beta-glukozidazom i/ili jednom ili više dodatnih celobiohidrolaza i/ili jednu ili više ksinalazu.
14. Upotreba polipeptida u skladu s jednim ili više patentnih zahtjeva 1 do 9 ili pripravka u skladu s patentnim zahtjevom 13, naznačena time, da je namijenjena enzimskoj razgradnji biomase lignoceluloze i/ili za obradu tekstila i/ili kao sastojak u deterdžentima i/ili kao sastojak u hrani ili pripravcima stočne hrane.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10153355.2A EP2357227B1 (en) | 2010-02-11 | 2010-02-11 | Optimized cellulase enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20151043T1 true HRP20151043T1 (hr) | 2015-11-06 |
Family
ID=42027636
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20151043TT HRP20151043T1 (hr) | 2010-02-11 | 2015-10-01 | Optimizirani celulaza enzimi |
| HRP20181587TT HRP20181587T1 (hr) | 2010-02-11 | 2018-10-03 | Optimizirani celulaza enzimi |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20181587TT HRP20181587T1 (hr) | 2010-02-11 | 2018-10-03 | Optimizirani celulaza enzimi |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9458443B2 (hr) |
| EP (2) | EP2357227B1 (hr) |
| CN (1) | CN102844430A (hr) |
| AU (1) | AU2011214298B2 (hr) |
| BR (1) | BR112012020277A2 (hr) |
| CA (1) | CA2789423C (hr) |
| DK (2) | DK2357227T3 (hr) |
| ES (2) | ES2549328T3 (hr) |
| HR (2) | HRP20151043T1 (hr) |
| MX (1) | MX344963B (hr) |
| PL (2) | PL2357227T3 (hr) |
| RS (1) | RS57695B1 (hr) |
| SI (2) | SI2357227T1 (hr) |
| WO (1) | WO2011098551A2 (hr) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102884086B (zh) | 2010-06-01 | 2015-11-25 | 加州理工学院 | 稳定的功能嵌合纤维二糖水解酶i类酶 |
| BR112013008048A2 (pt) | 2010-10-06 | 2016-06-14 | Bp Corp North America Inc | polipeptídeos, composição, caldo de fermentação, métodos para sacrificar biomassa e para produzir etanol, ácido nucleico, vetor, células recombinante e hospedeira e métodos para produzir polipeptídeo e para gerar polipeptídeo de cbh i variante tolerante a produto e ácido nucleíco |
| US20140080182A1 (en) * | 2011-03-31 | 2014-03-20 | Novozymes, Inc. | Cellulose Binding Domain Variants and Polynucleotides Encoding Same |
| EP2780450A4 (en) * | 2011-11-15 | 2015-09-09 | Novozymes Inc | POLYPEPTIDES HAVING CELLOBIOHYDROLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME |
| BR112014015242B1 (pt) * | 2011-12-22 | 2023-01-31 | Novozymes, Inc | Polipeptídeo híbrido isolado, polinucleotídeo isolado,célula hospedeira, método de produção de um polipeptídeo híbrido, processos para degradação ou conversão de um material celulósico, para produção de um produto de fermentação, de fermentação de um material celulósico, formulação ou composição de cultura de células microbianas de caldo completo, e,célula hospedeira microbiana transgênica |
| MX2015007234A (es) | 2012-12-12 | 2015-10-29 | Danisco Us Inc | Variantes de celobiohidrolasas. |
| BR112015013646A2 (pt) * | 2012-12-12 | 2017-11-14 | Danisco Us Inc | variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular |
| EP2964760B1 (en) * | 2013-03-08 | 2021-05-12 | Novozymes A/S | Cellobiohydrolase variants and polynucleotides encoding same |
| BR112017004251A2 (pt) | 2014-09-05 | 2017-12-12 | Novozymes As | variantes de módulos de ligação a carboidrato e polinucleotídeos que os codificam |
| EP3191587B1 (en) | 2014-09-08 | 2019-07-17 | Novozymes A/S | Cellobiohydrolase variants and polynucleotides encoding same |
| JP6378622B2 (ja) * | 2014-12-04 | 2018-08-22 | 本田技研工業株式会社 | 高活性セロビオヒドロラーゼ |
| WO2016138167A2 (en) | 2015-02-24 | 2016-09-01 | Novozymes A/S | Cellobiohydrolase variants and polynucleotides encoding same |
| WO2017177289A1 (en) | 2016-04-15 | 2017-10-19 | Vtt International Oy | Process for conversion of biomass into fermentable sugars with integrated enzyme |
| EA201990035A1 (ru) | 2016-06-15 | 2019-06-28 | Кодексис, Инк. | Генно-инженерные бета-глюкозидазы и способы глюкозилирования |
| CN109337888B (zh) * | 2018-11-12 | 2021-08-17 | 南开大学 | 纤维二糖水解酶突变体及其生产方法和应用 |
| CN110373402B (zh) * | 2019-08-13 | 2021-07-20 | 山东农业大学 | 一种提升耐热纤维素酶酶活力和热稳定性的方法 |
| CN113186175B (zh) * | 2021-06-04 | 2023-08-04 | 翌圣生物科技(上海)股份有限公司 | 突变型Taq DNA聚合酶、编码DNA序列、重组载体、重组表达细胞及其应用 |
| CN114426961B (zh) * | 2022-03-10 | 2024-01-26 | 安徽大学 | 一种β-葡萄糖苷酶突变体、其编码基因、表达菌株及其应用 |
| CN117802072B (zh) * | 2024-02-29 | 2024-05-24 | 北京挑战生物技术有限公司 | 木聚糖酶突变体及其应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK115890D0 (da) | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
| US7375197B2 (en) * | 2002-01-14 | 2008-05-20 | Midwest Research Institute | Cellobiohydrolase I gene and improved variants |
| US8637293B2 (en) | 1999-07-13 | 2014-01-28 | Alliance For Sustainable Energy, Llc | Cellobiohydrolase I enzymes |
| EP1200574A4 (en) | 1999-07-13 | 2004-03-17 | Midwest Research Inst | REDUCED GLYCOSYLATION VARIANTS OF CELLOBIOHYDROLASE: CBHIN45A, CBHIN270A AND CBHIN384A |
| EP2295544B1 (en) | 2001-06-26 | 2016-06-22 | Novozymes A/S | Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same |
| EP2322607B1 (en) | 2002-08-16 | 2015-09-16 | Danisco US Inc. | Novel variant Hyprocrea jecorina CBH1 cellulases with increase thermal stability comprising substitution or deletion at position S113 |
| AU2003219956A1 (en) * | 2003-02-27 | 2004-09-28 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
| EP2298876A1 (en) | 2003-03-21 | 2011-03-23 | Genencor International, Inc. | Novel cbh1 homologs and variant cbh1 cellulases |
| JP5427342B2 (ja) | 2003-04-01 | 2014-02-26 | ジェネンコー・インターナショナル・インク | 変異体フミコーラ・グリセアｃｂｈ１．１ |
| DK2377931T3 (da) | 2003-08-25 | 2013-07-08 | Novozymes Inc | Varianter af glycosidhydrolase |
| DK1874927T3 (da) * | 2005-04-29 | 2014-04-22 | Ab Enzymes Oy | Forbedrede cellulaser |
| CN1757710A (zh) | 2005-07-15 | 2006-04-12 | 山东农业大学 | 表达cbh1基因的酵母工程菌株及其构建方法 |
| FI120045B (fi) | 2005-12-22 | 2009-06-15 | Roal Oy | Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit |
| US8017373B2 (en) * | 2006-08-31 | 2011-09-13 | Iogen Energy Corporation | Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks |
| EP2260105B1 (en) | 2008-02-29 | 2016-08-17 | The Trustees Of The University Of Pennsylvania | Production and use of plant degrading materials |
| CA3021166C (en) * | 2008-05-11 | 2024-01-09 | Universiteit Stellenbosch | Heterologous expression of fungal cellobiohydrolases in yeast |
| CA2724036C (en) * | 2008-05-11 | 2018-09-25 | Mascoma Corporation | Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases |
| CA2729924C (en) * | 2008-07-07 | 2018-05-29 | Mascoma Corporation | Isolation and characterization of schizochytrium aggregatum cellobiohydrolase i (cbh 1) |
| EP2421965B1 (en) | 2009-04-24 | 2016-05-25 | DSM IP Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
-
2010
- 2010-02-11 DK DK10153355.2T patent/DK2357227T3/en active
- 2010-02-11 EP EP10153355.2A patent/EP2357227B1/en active Active
- 2010-02-11 ES ES10153355.2T patent/ES2549328T3/es active Active
- 2010-02-11 PL PL10153355T patent/PL2357227T3/pl unknown
- 2010-02-11 SI SI201031027T patent/SI2357227T1/sl unknown
-
2011
- 2011-02-11 CN CN2011800185464A patent/CN102844430A/zh active Pending
- 2011-02-11 WO PCT/EP2011/052023 patent/WO2011098551A2/en active Application Filing
- 2011-02-11 PL PL11706777T patent/PL2534245T3/pl unknown
- 2011-02-11 ES ES11706777.7T patent/ES2689939T3/es active Active
- 2011-02-11 DK DK11706777.7T patent/DK2534245T3/en active
- 2011-02-11 SI SI201131575T patent/SI2534245T1/sl unknown
- 2011-02-11 CA CA2789423A patent/CA2789423C/en active Active
- 2011-02-11 AU AU2011214298A patent/AU2011214298B2/en active Active
- 2011-02-11 BR BR112012020277A patent/BR112012020277A2/pt not_active IP Right Cessation
- 2011-02-11 US US13/578,291 patent/US9458443B2/en active Active
- 2011-02-11 MX MX2012009357A patent/MX344963B/es active IP Right Grant
- 2011-02-11 RS RS20181132A patent/RS57695B1/sr unknown
- 2011-02-11 EP EP11706777.7A patent/EP2534245B1/en active Active
-
2015
- 2015-10-01 HR HRP20151043TT patent/HRP20151043T1/hr unknown
-
2018
- 2018-10-03 HR HRP20181587TT patent/HRP20181587T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011098551A3 (en) | 2012-05-31 |
| CA2789423A1 (en) | 2011-08-18 |
| US9458443B2 (en) | 2016-10-04 |
| AU2011214298B2 (en) | 2014-11-06 |
| EP2534245B1 (en) | 2018-07-04 |
| WO2011098551A2 (en) | 2011-08-18 |
| SI2357227T1 (sl) | 2015-11-30 |
| MX2012009357A (es) | 2012-12-17 |
| BR112012020277A2 (pt) | 2015-09-15 |
| PL2357227T3 (pl) | 2015-12-31 |
| EP2357227A1 (en) | 2011-08-17 |
| MX344963B (es) | 2017-01-11 |
| EP2534245A2 (en) | 2012-12-19 |
| DK2534245T3 (en) | 2018-10-01 |
| SI2534245T1 (sl) | 2019-01-31 |
| AU2011214298A1 (en) | 2012-09-06 |
| HRP20181587T1 (hr) | 2018-12-14 |
| US20130123115A1 (en) | 2013-05-16 |
| CA2789423C (en) | 2017-12-12 |
| EP2357227B1 (en) | 2015-07-15 |
| PL2534245T3 (pl) | 2019-02-28 |
| CN102844430A (zh) | 2012-12-26 |
| RS57695B1 (sr) | 2018-11-30 |
| ES2549328T3 (es) | 2015-10-27 |
| DK2357227T3 (en) | 2015-09-14 |
| ES2689939T3 (es) | 2018-11-16 |
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