DK3191587T3 - Cellobiohydrolasevarianter og polynukleotider, som koder for dem - Google Patents

Cellobiohydrolasevarianter og polynukleotider, som koder for dem

Info

Publication number
DK3191587T3
DK3191587T3 DK15767641.2T DK15767641T DK3191587T3 DK 3191587 T3 DK3191587 T3 DK 3191587T3 DK 15767641 T DK15767641 T DK 15767641T DK 3191587 T3 DK3191587 T3 DK 3191587T3
Authority
DK
Denmark
Prior art keywords
polynucleotides encoding
cellobiohydrolase variants
cellobiohydrolase
variants
polynucleotides
Prior art date
Application number
DK15767641.2T
Other languages
English (en)
Inventor
Kim Borch
Tine Sorensen
Michael Windahl
Brett Mcbrayer
Peter Westh
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK3191587T3 publication Critical patent/DK3191587T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK15767641.2T 2014-09-08 2015-09-08 Cellobiohydrolasevarianter og polynukleotider, som koder for dem DK3191587T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462047296P 2014-09-08 2014-09-08
PCT/US2015/048850 WO2016040265A1 (en) 2014-09-08 2015-09-08 Cellobiohydrolase variants and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
DK3191587T3 true DK3191587T3 (da) 2019-10-14

Family

ID=54186288

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15767641.2T DK3191587T3 (da) 2014-09-08 2015-09-08 Cellobiohydrolasevarianter og polynukleotider, som koder for dem

Country Status (5)

Country Link
US (1) US10676769B2 (da)
EP (1) EP3191587B1 (da)
BR (1) BR112017004495A2 (da)
DK (1) DK3191587T3 (da)
WO (1) WO2016040265A1 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013019039A2 (pt) * 2011-01-26 2020-07-21 Novozymes A/S Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula originária, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, 5 e parafermentar um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado
WO2018178991A1 (en) * 2017-03-29 2018-10-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Purification of an agent of interest

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7375197B2 (en) 2002-01-14 2008-05-20 Midwest Research Institute Cellobiohydrolase I gene and improved variants
US8637293B2 (en) * 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
DK1578943T3 (da) 2002-08-16 2012-01-09 Danisco Us Inc Nye variant-Hypocrea jecorina-CBH1-cellulaser
JP2007534294A (ja) 2003-03-21 2007-11-29 ジェネンコー・インターナショナル・インク Ｃｂｈ１相同体及び変異体ｃｂｈ１セルラーゼ
CA2771875A1 (en) 2003-04-01 2005-01-06 Danisco Us Inc. Variant hypocrea jecorina cbh1
CN103088001A (zh) 2003-08-25 2013-05-08 诺维信股份有限公司 糖苷水解酶的变体
RU2458127C2 (ru) 2006-04-13 2012-08-10 Аб Энзимз Оу Целлюлозные белки слияния и их применение
AU2009316309B2 (en) 2008-11-21 2016-07-07 Lallemand Hungary Liquidity Management Llc Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose
MX2011009009A (es) 2009-02-27 2011-09-15 Iogen Energy Corp Enzimas de celulosa resistentes a lignina.
WO2011050037A1 (en) 2009-10-23 2011-04-28 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
ES2549328T3 (es) 2010-02-11 2015-10-27 Süd-Chemie Ip Gmbh & Co. Kg Enzimas celulasas optimizadas
BR112012020804A2 (pt) 2010-02-11 2015-09-15 Iogen Energy Corp módulos de ligação de carboidrato com ligação reduzida à lignina
US9096871B2 (en) 2010-10-06 2015-08-04 Bp Corporation North America Inc. Variant CBH I polypeptides with reduced product inhibition
MX340248B (es) 2011-01-31 2016-07-01 Dsm Ip Assets Bv Celobiohidrolasa mutante.
WO2012135719A1 (en) 2011-03-31 2012-10-04 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
US9279163B2 (en) 2011-08-31 2016-03-08 Iogen Energy Corporation Cellobiohydrolase enzymes
DK2794664T3 (da) 2011-12-22 2017-08-21 Novozymes Inc Hybridpolypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
WO2013138357A1 (en) 2012-03-12 2013-09-19 Codexis, Inc. Cbh1a variants
US20150291947A1 (en) 2012-10-23 2015-10-15 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
US20160122735A1 (en) 2012-12-12 2016-05-05 Danisco Us Inc. Variants of cellobiohydrolases
CA2893059A1 (en) 2012-12-12 2014-06-19 Danisco Us Inc. Variants of cellobiohydrolases
EP2964760B1 (en) 2013-03-08 2021-05-12 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same

Also Published As

Publication number Publication date
WO2016040265A1 (en) 2016-03-17
EP3191587A1 (en) 2017-07-19
BR112017004495A2 (pt) 2018-02-27
EP3191587B1 (en) 2019-07-17
US20170292136A1 (en) 2017-10-12
US10676769B2 (en) 2020-06-09

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