MX2021014368A - Metodos y composiciones para la modificacion dirigida de un genoma. - Google Patents

Metodos y composiciones para la modificacion dirigida de un genoma.

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Publication number
MX2021014368A
MX2021014368A MX2021014368A MX2021014368A MX2021014368A MX 2021014368 A MX2021014368 A MX 2021014368A MX 2021014368 A MX2021014368 A MX 2021014368A MX 2021014368 A MX2021014368 A MX 2021014368A MX 2021014368 A MX2021014368 A MX 2021014368A
Authority
MX
Mexico
Prior art keywords
methods
compositions
genome
human
cell
Prior art date
Application number
MX2021014368A
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English (en)
Inventor
David Frendewey
Junko Kuno
David M Valenzuela
Wojtek Auerbach
George D Yancopoulos
Ka- Man Venus LAI
Original Assignee
Regeneron Pharma
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Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2021014368A publication Critical patent/MX2021014368A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
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    • A01K2217/00—Genetically modified animals
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    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2217/00—Genetically modified animals
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    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
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    • A01K2267/03—Animal model, e.g. for test or diseases
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    • A01K2267/0362—Animal model for lipid/glucose metabolism, e.g. obesity, type-2 diabetes
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N2800/00—Nucleic acids vectors
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    • C12N2810/00—Vectors comprising a targeting moiety
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    • C12N2810/00—Vectors comprising a targeting moiety
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    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
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    • C12N2999/00—Further aspects of viruses or vectors not covered by groups C12N2710/00 - C12N2796/00 or C12N2800/00
    • C12N2999/007—Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals

Abstract

Se proporcionan composiciones y métodos para modificar un locus genómico de interés en una célula eucariota, una célula de mamífero, una célula humana o una célula de mamífero no humano utilizando un vector de direccionamiento grande (LTVEC) que comprende varias secuencias de ácido nucleico endógeno o exógeno tal como se describen en la presente. Métodos adicionales combinan el uso del LTVEC con un sistema CRISPR/Cas. También se proporcionan composiciones y métodos para generar un animal no humano modificado genéticamente que comprende una o más modificaciones genéticas dirigidas en su línea germinal.
MX2021014368A 2013-12-11 2016-06-10 Metodos y composiciones para la modificacion dirigida de un genoma. MX2021014368A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201361914768P 2013-12-11 2013-12-11
US201462017416P 2014-06-26 2014-06-26
US201462029261P 2014-07-25 2014-07-25
US201462052906P 2014-09-19 2014-09-19
US201462059527P 2014-10-03 2014-10-03
US201462064384P 2014-10-15 2014-10-15

Publications (1)

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MX2021014368A true MX2021014368A (es) 2022-01-06

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MX2016007654A MX2016007654A (es) 2013-12-11 2014-10-15 Metodos y composiciones para la modificacion dirigida de un genoma.
MX2021014368A MX2021014368A (es) 2013-12-11 2016-06-10 Metodos y composiciones para la modificacion dirigida de un genoma.

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US (7) US9546384B2 (es)
JP (3) JP6174811B2 (es)
KR (2) KR102170502B1 (es)
CN (2) CN105980568B (es)
AU (3) AU2014360811B2 (es)
BR (1) BR112016013400B1 (es)
CA (1) CA2933433C (es)
IL (1) IL245674B (es)
MX (2) MX2016007654A (es)
RU (2) RU2725520C2 (es)
SG (1) SG10201700961TA (es)
WO (1) WO2015088643A1 (es)

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