KR830003574A - 변칙합성 유전자의 미생물을 통한 발현 - Google Patents
변칙합성 유전자의 미생물을 통한 발현 Download PDFInfo
- Publication number
- KR830003574A KR830003574A KR1019800002658A KR800002658A KR830003574A KR 830003574 A KR830003574 A KR 830003574A KR 1019800002658 A KR1019800002658 A KR 1019800002658A KR 800002658 A KR800002658 A KR 800002658A KR 830003574 A KR830003574 A KR 830003574A
- Authority
- KR
- South Korea
- Prior art keywords
- polypeptide
- gene
- growth hormone
- amino acids
- human growth
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 11
- 238000003786 synthesis reaction Methods 0.000 title claims description 4
- 230000000813 microbial effect Effects 0.000 title claims 4
- 230000015572 biosynthetic process Effects 0.000 title description 2
- 230000002547 anomalous effect Effects 0.000 title 1
- 239000012634 fragment Substances 0.000 claims description 9
- 102000002265 Human Growth Hormone Human genes 0.000 claims description 7
- 108010000521 Human Growth Hormone Proteins 0.000 claims description 7
- 239000000854 Human Growth Hormone Substances 0.000 claims description 7
- 150000001413 amino acids Chemical class 0.000 claims description 6
- 238000010367 cloning Methods 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 108020004999 messenger RNA Proteins 0.000 claims description 4
- 239000013612 plasmid Substances 0.000 claims description 4
- 229920001184 polypeptide Polymers 0.000 claims 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims 10
- 239000000047 product Substances 0.000 claims 4
- 108020004705 Codon Proteins 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 238000000855 fermentation Methods 0.000 claims 2
- 230000004151 fermentation Effects 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 235000015097 nutrients Nutrition 0.000 claims 2
- 239000006228 supernatant Substances 0.000 claims 2
- 108091026890 Coding region Proteins 0.000 claims 1
- 241000721047 Danaus plexippus Species 0.000 claims 1
- 238000005273 aeration Methods 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 230000002934 lysing effect Effects 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 206010062767 Hypophysitis Diseases 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 210000003635 pituitary gland Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/12—Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
도면 1 : 인간의 성장호르몬의 처음 24개의 아미노산에 대한 유전자와 출발신호인 ATG 그리고 클로닝시에 필요한 링커를 제조하는 데 대한 합성도이다. 상부가닥(암호용)과 하부 가닥(보완용) 중에 있는 화살표는 유전자 단편을 제조키 위해 결합된 올리고뉴클레오타이드를 나타내고 있다.
도면 2 : 도면 1의 유존자단편을 형성키 위한 "U"와 "L" 올리고뉴클레오타이드의 결합체와 이것을 플라스미드클로닝 담체로 삽입하는 것이 나타나 있다.
도면 3 : 뇌하수체 mRNA 복사의 Hae Ⅲ 제한 효소에 대한 DNA 순서(암호 가닥만)와 이들이 암호하는 인간성장 호르몬의 아미노산이 나타나 있다. 비번역 mRNA에 대한 DNA("중지"에 연속)에서 처럼 주요한 제한부위가 나타나 있다.
도면 4 : 합성으로 제조하지 않은 인간의 성장호르몬의 아미노산에 대한 유전자 단편에 대한 클로닝 담체 제조와 인간 뇌하수체원에서 분리된 mRNA로 부터 역복사법에 의해 보완 DNa 로서의 유전자단편의 제조에 관한 것이다.
도면 5 : 박테리아에서 도면 2와 3의 플라스미드로 시작하는 인간성장호르몬을 발현할 수 있는 플라스미드의 제조에 대해 나타나 있다.
Claims (2)
- 아미노산 순서가 알려진 폴리펩타이이드에 대한 유전자 암호를 클로닝담체에 삽입하고 발현 푸로모터의 조절하에 미생물 계에서 발현시켜서 목적하는 폴리펩타이드를 다음과 같은 구체적인 방법을 통하여 제조하는 방법.a) mRNA를 역복사시켜서 적어도 상기 폴리펩타이드의 암호순서의 상당부분을 이루며, 목적폴리펩타이드보다는 발현산물에 대한 최초유전자의 단편을 얻는다.b) 만일에 최초단편에 상기 폴리펩타이드에 함유하는 이외의 아미노산에 대한 코돈이 들어있으면 적어도 상기암호순서의 상당부분을 함유하도록 하면서 이 불필요한 코돈을 어느 정도 제거하여 아직 상기 폴리펩타이드이외의 발현산물에 대한 단편을 얻는다.((a) 단계의 산물이나 필요할 때는 (b) 단계의 산물인 유전자 단편중에는 상기 폴리펩타이드의 모든 아미노산보다 적은 암호유전자가 함유될 수도 있다.)(c) 유기합성법에 의하여 상기 폴리펩타이드의 나머지 아미노산에 대한 유전자단편을 얻는데 이중에는 적어도 한단편에는 폴리펩타이드의 아미노만단 부위를 암호하는 유전자가 함유되어 있다.d) 상기 c)단계에서 유기합성법으로 얻은 유전자와 a)나 b)단계에 얻은 유전자를 적절히 해독할 수 있는 순서로 조합하고 발현 푸로모터의 조절하에 있도록 복사능이 있는 클로닝 담체에 장치하며, 이렇게하여 상기 폴리펩타이드의 아미노산 순서를 전부 발현시킬 수 있는 클로닝담체를 얻는다.
- 다음의 방법을 사용하여 인간의 성장호르몬을 제조 방법 :a) 통기와 진탕을 시키게 되어 있는 발효조 내의 수성의 영양분 함유발효액에다 인간의 성장호르몬을 발현시킬 수 있으며 복사능이 있는 플라스미드를 함유하는 박테리아 균주를 가하여b) 왕성한 성장을 유지시키기 위해 필요하면 영양분을 더 제공하고 통기와 진탕을 하면서 군주를 증식시켜서c) 결과적으로 얻어지는 미생물 세포들을 발효액으로 부터 분리하여d) 미생물 세포를 용균시켜e) 세포잔해를 상등액으로부터 분리하여f) 상등액중에 들어 있는 인간의 성장호르몬을 분리한다.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019860009542A KR870000701B1 (ko) | 1979-07-05 | 1986-11-12 | 인체 성장 호르몬의 제조방법 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/055,126 US4342832A (en) | 1979-07-05 | 1979-07-05 | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
US55126 | 1979-07-05 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019860009542A Division KR870000701B1 (ko) | 1979-07-05 | 1986-11-12 | 인체 성장 호르몬의 제조방법 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR830003574A true KR830003574A (ko) | 1983-06-21 |
Family
ID=26733870
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019800002658A KR830003574A (ko) | 1979-07-05 | 1980-07-04 | 변칙합성 유전자의 미생물을 통한 발현 |
KR1019860009542A KR870000701B1 (ko) | 1979-07-05 | 1986-11-12 | 인체 성장 호르몬의 제조방법 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019860009542A KR870000701B1 (ko) | 1979-07-05 | 1986-11-12 | 인체 성장 호르몬의 제조방법 |
Country Status (42)
Country | Link |
---|---|
US (7) | US4342832A (ko) |
EP (1) | EP0022242B1 (ko) |
JP (4) | JPH0612996B2 (ko) |
KR (2) | KR830003574A (ko) |
AR (1) | AR244341A1 (ko) |
AT (1) | ATE82324T1 (ko) |
AU (2) | AU533697B2 (ko) |
BE (1) | BE884012A (ko) |
BG (1) | BG41135A3 (ko) |
BR (1) | BR8008736A (ko) |
CA (2) | CA1164375A (ko) |
CH (1) | CH661939A5 (ko) |
CS (3) | CS250652B2 (ko) |
DD (3) | DD210071A5 (ko) |
DE (3) | DE3050722C2 (ko) |
DK (2) | DK173503B1 (ko) |
EG (1) | EG14819A (ko) |
ES (2) | ES493149A0 (ko) |
FI (2) | FI802030A (ko) |
FR (2) | FR2460330B1 (ko) |
GB (2) | GB2121047B (ko) |
GR (1) | GR69320B (ko) |
HK (3) | HK87484A (ko) |
IE (3) | IE50462B1 (ko) |
IL (3) | IL69492A (ko) |
IT (1) | IT1131393B (ko) |
KE (3) | KE3450A (ko) |
MX (1) | MX172674B (ko) |
MY (3) | MY8500763A (ko) |
NL (1) | NL930114I2 (ko) |
NO (2) | NO167673C (ko) |
NZ (2) | NZ201312A (ko) |
OA (1) | OA06562A (ko) |
PH (1) | PH19814A (ko) |
PL (1) | PL149278B1 (ko) |
PT (1) | PT71487A (ko) |
RO (1) | RO93374B (ko) |
SG (1) | SG56984G (ko) |
WO (1) | WO1981000114A1 (ko) |
YU (3) | YU163580A (ko) |
ZA (1) | ZA803600B (ko) |
ZW (1) | ZW14180A1 (ko) |
Families Citing this family (186)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
US4898830A (en) * | 1979-07-05 | 1990-02-06 | Genentech, Inc. | Human growth hormone DNA |
US6455275B1 (en) | 1980-02-25 | 2002-09-24 | The Trustees Of Columbia University In The City Of New York | DNA construct for producing proteinaceous materials in eucaryotic cells |
CA1200773A (en) * | 1980-02-29 | 1986-02-18 | William J. Rutter | Expression linkers |
US4711843A (en) * | 1980-12-31 | 1987-12-08 | Cetus Corporation | Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis |
IL59690A (en) * | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
ZA811368B (en) * | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
US4370417A (en) * | 1980-04-03 | 1983-01-25 | Abbott Laboratories | Recombinant deoxyribonucleic acid which codes for plasminogen activator |
US6610830B1 (en) | 1980-07-01 | 2003-08-26 | Hoffman-La Roche Inc. | Microbial production of mature human leukocyte interferons |
IE53166B1 (en) * | 1980-08-05 | 1988-08-03 | Searle & Co | Synthetic urogastrone gene,corresponding plasmid recombinants,transformed cells,production thereof and urogastrone expression |
US7101981B1 (en) | 1980-08-26 | 2006-09-05 | Regents Of The University Of California | Bovine growth hormone recombinantly produced in E. coli |
US4725549A (en) * | 1980-09-22 | 1988-02-16 | The Regents Of The University Of California | Human and rat prolactin and preprolactin cloned genes |
NZ198445A (en) * | 1980-09-25 | 1984-05-31 | Genentech Inc | Production of human fibroblast interferon by recombinant dna technology |
NZ199722A (en) * | 1981-02-25 | 1985-12-13 | Genentech Inc | Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain |
JPS57181098A (en) * | 1981-04-30 | 1982-11-08 | Japan Found Cancer | Novel recombinant dna |
US4801685A (en) * | 1981-08-14 | 1989-01-31 | Hoffmann-La Roche Inc. | Microbial production of mature human leukocyte interferon K and L |
US4810645A (en) * | 1981-08-14 | 1989-03-07 | Hoffmann-La Roche Inc. | Microbial production of mature human leukocyte interferon K and L |
US4880910A (en) * | 1981-09-18 | 1989-11-14 | Genentech, Inc. | Terminal methionyl bovine growth hormone and its use |
NZ201918A (en) * | 1981-09-18 | 1987-04-30 | Genentech Inc | N-terminal methionyl analogues of bovine growth hormone |
US5254463A (en) * | 1981-09-18 | 1993-10-19 | Genentech, Inc. | Method for expression of bovine growth hormone |
US5236831A (en) * | 1981-12-29 | 1993-08-17 | Kiowa Hakko Kogyo Co., Ltd. | Amino acid synthesis in corynebacteria using E. coli genes |
US4775622A (en) * | 1982-03-08 | 1988-10-04 | Genentech, Inc. | Expression, processing and secretion of heterologous protein by yeast |
US4446235A (en) * | 1982-03-22 | 1984-05-01 | Genentech, Inc. | Method for cloning human growth hormone varient genes |
US4665160A (en) * | 1982-03-22 | 1987-05-12 | Genentech, Inc. | Novel human growth hormone like protein HGH-V encoded in the human genome |
US6936694B1 (en) * | 1982-05-06 | 2005-08-30 | Intermune, Inc. | Manufacture and expression of large structural genes |
US4652639A (en) * | 1982-05-06 | 1987-03-24 | Amgen | Manufacture and expression of structural genes |
GB2121054B (en) * | 1982-05-25 | 1986-02-26 | Lilly Co Eli | Cloning vectors for expression of exogenous protein |
US4778759A (en) * | 1982-07-09 | 1988-10-18 | Boyce, Thompson Institute For Plant Research, Inc. | Genetic engineering in cyanobacteria |
US4891315A (en) * | 1982-10-25 | 1990-01-02 | American Cyanamid Company | Production of herpes simplex viral porteins |
EP0116631A4 (en) * | 1982-08-10 | 1986-04-15 | Univ Columbia | USE OF EUKARYOTIC ACTIVATING SEQUENCES IN THE PRODUCTION OF PROTEIN MATERIALS. |
US4530904A (en) * | 1982-09-03 | 1985-07-23 | Eli Lilly And Company | Method for conferring bacteriophage resistance to bacteria |
CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
WO1984001150A1 (en) * | 1982-09-16 | 1984-03-29 | Amgen | Avian growth hormones |
US4666839A (en) * | 1982-12-01 | 1987-05-19 | Amgen | Methods and materials for obtaining microbial expression of polypeptides including bovine prolactin |
JPS59106297A (ja) * | 1982-12-07 | 1984-06-19 | Rikagaku Kenkyusho | ヒト生長ホルモンのカルボキシ末端遺伝子の合成法 |
US5618697A (en) * | 1982-12-10 | 1997-04-08 | Novo Nordisk A/S | Process for preparing a desired protein |
DK55685A (da) * | 1985-02-07 | 1986-08-08 | Nordisk Gentofte | Enzym eller enzymkompleks med proteolytisk aktivitet |
US4634678A (en) * | 1982-12-13 | 1987-01-06 | Molecular Genetics Research And Development Limited Partnership | Plasmid cloning and expression vectors for use in microorganisms |
GB8303383D0 (en) * | 1983-02-08 | 1983-03-16 | Biogen Nv | Sequences recombinant dna molecules |
US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
US4859600A (en) * | 1983-04-25 | 1989-08-22 | Genentech, Inc. | Recombinant procaryotic cell containing correctly processed human growth hormone |
US4755465A (en) * | 1983-04-25 | 1988-07-05 | Genentech, Inc. | Secretion of correctly processed human growth hormone in E. coli and Pseudomonas |
IL71991A (en) | 1983-06-06 | 1994-05-30 | Genentech Inc | Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes |
BG49718A3 (en) * | 1983-07-15 | 1992-01-15 | Bio Technology General Corp | Method for preparing of polypeptid with superoxiddismutasne activitty |
US4900811A (en) * | 1983-07-21 | 1990-02-13 | Scripps Clinic And Research Foundation | Synthetic polypeptides corresponding to portions of proteinoids translated from brain-specific mRNAs, receptors, methods and diagnostics using the same |
US5242798A (en) * | 1983-07-21 | 1993-09-07 | Scripps Clinic And Research Foundation | Synthetic polypeptides corresponding to portions of proteinoids translated from brain-specific mRNAs, receptors, methods and diagnostics using the same |
JPS60137291A (ja) * | 1983-12-26 | 1985-07-20 | Takeda Chem Ind Ltd | 発現ベクター |
CA1213537A (en) * | 1984-05-01 | 1986-11-04 | Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee | Polypeptide expression method |
CA1272144A (en) * | 1984-06-29 | 1990-07-31 | Tamio Mizukami | Fish growth hormone polypeptide |
US5489529A (en) * | 1984-07-19 | 1996-02-06 | De Boer; Herman A. | DNA for expression of bovine growth hormone |
WO1986002068A1 (en) * | 1984-09-26 | 1986-04-10 | Takeda Chemical Industries, Ltd. | Mutual separation of proteins |
EP0177343B1 (en) * | 1984-10-05 | 1992-07-22 | Genentech, Inc. | Dna, cell cultures and methods for the secretion of heterologous proteins and periplasmic protein recovery |
US4680262A (en) * | 1984-10-05 | 1987-07-14 | Genentech, Inc. | Periplasmic protein recovery |
NZ213759A (en) * | 1984-10-19 | 1989-01-27 | Genentech Inc | Lhrh-ctp protein conjugates influencing prolactin fsh and lh release |
US4645829A (en) * | 1984-10-29 | 1987-02-24 | Monsanto Company | Method for separating polypeptides |
US4861868A (en) * | 1985-02-22 | 1989-08-29 | Monsanto Company | Production of proteins in procaryotes |
US4652630A (en) * | 1985-02-22 | 1987-03-24 | Monsanto Company | Method of somatotropin naturation |
DK151585D0 (da) * | 1985-04-03 | 1985-04-03 | Nordisk Gentofte | Dna-sekvens |
DE3683186D1 (de) | 1985-04-25 | 1992-02-13 | Hoffmann La Roche | Rekombinantes humaninterleukin-1. |
IL79289A (en) * | 1985-07-05 | 1992-01-15 | Whitehead Biomedical Inst | Introduction and expression of foreign genetic material into keratinocytes using a recombinant retrovirus |
US5041381A (en) | 1986-07-03 | 1991-08-20 | Schering Corporation | Monoclonal antibodies against human interleukin-4 and hybridomas producing the same |
US4892764A (en) * | 1985-11-26 | 1990-01-09 | Loctite Corporation | Fiber/resin composites, and method of making the same |
US5552528A (en) * | 1986-03-03 | 1996-09-03 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Bovine b-endothelial cell growth factor |
US5827826A (en) * | 1986-03-03 | 1998-10-27 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Compositions of human endothelial cell growth factor |
ES2061524T3 (es) * | 1986-05-07 | 1994-12-16 | Eniricerche Spa | Un vector plasmidico para la expresion en bacillus y empleado para la clonacion del gen estructural que codifica la hormona de crecimiento humana y un metodo para la produccion de la hormona. |
EP0252588A3 (en) * | 1986-05-12 | 1989-07-12 | Smithkline Beecham Corporation | Process for the isolation and purification of p. falciparum cs protein expressed in recombinant e. coli, and its use as a vaccine |
US4806239A (en) * | 1986-11-28 | 1989-02-21 | Envirotech Corporation | Apparatus for shifting filter plates in a filter press |
JPS63159244A (ja) * | 1986-12-23 | 1988-07-02 | 三菱マテリアル株式会社 | 二層の押出成形珪酸質―石灰質系成形品の製造方法 |
ATE119573T1 (de) * | 1986-12-31 | 1995-03-15 | Lucky Ltd | Verfahren zur herstellung eines lachswachstumshormons mittels eines kunstgens. |
JPH02502604A (ja) * | 1987-01-07 | 1990-08-23 | アライド・コーポレーション | ペプチドオリゴマーの微生物生産法 |
US4977089A (en) * | 1987-01-30 | 1990-12-11 | Eli Lilly And Company | Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms |
WO1988010119A1 (en) * | 1987-06-22 | 1988-12-29 | Genetics Institute, Inc. | Novel thrombolytic proteins |
FR2624835B2 (fr) * | 1987-08-05 | 1990-09-07 | Hassevelde Roger | Support ergonomique a creme glacee, a cuillere integree, transformable en tout autre objet apres son utilisation premiere |
US5268267A (en) * | 1987-08-21 | 1993-12-07 | The General Hospital Corporation | Method for diagnosing small cell carcinoma |
IT1223577B (it) * | 1987-12-22 | 1990-09-19 | Eniricerche Spa | Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura |
JPH0231042U (ko) * | 1988-08-19 | 1990-02-27 | ||
US5130422A (en) * | 1988-08-29 | 1992-07-14 | Monsanto Company | Variant somatotropin-encoding DNA |
EP0763543A2 (en) | 1988-09-02 | 1997-03-19 | The Rockefeller University | A method for preparing an inflammatory cytokine (MIP-2) and diagnostic and therapeutic applications for the cytokine or its antibody |
US5079230A (en) * | 1988-09-12 | 1992-01-07 | Pitman-Moore, Inc. | Stable bioactive somatotropins |
US5082767A (en) * | 1989-02-27 | 1992-01-21 | Hatfield G Wesley | Codon pair utilization |
US5075227A (en) * | 1989-03-07 | 1991-12-24 | Zymogenetics, Inc. | Directional cloning |
US4960301A (en) * | 1989-03-27 | 1990-10-02 | Fry Steven A | Disposable liner for pickup truck beds |
US5164180A (en) | 1989-05-18 | 1992-11-17 | Mycogen Corporation | Bacillus thuringiensis isolates active against lepidopteran pests |
US5266477A (en) * | 1990-02-02 | 1993-11-30 | Pitman-Moore, Inc. | Monoclonal antibodies which differentiate between native and modified porcine somatotropins |
CA2041446A1 (en) | 1990-05-15 | 1991-11-16 | August J. Sick | Bacillus thuringiensis genes encoding novel dipteran-active toxins |
US5849694A (en) * | 1990-07-16 | 1998-12-15 | Synenki; Richard M. | Stable and bioactive modified porcine somatotropin and pharmaceutical compositions thereof |
US5202119A (en) * | 1991-06-28 | 1993-04-13 | Genentech, Inc. | Method of stimulating immune response |
US5744139A (en) * | 1991-06-28 | 1998-04-28 | University Of Tennessee Research Corporation | Insulin-like growth factor I (IGF-1) induced improvement of depressed T4/T8 ratios |
US5591709A (en) * | 1991-08-30 | 1997-01-07 | Life Medical Sciences, Inc. | Compositions and methods for treating wounds |
HUT67319A (en) * | 1991-08-30 | 1995-03-28 | Life Medical Sciences Inc | Compositions for treating wounds |
US5317012A (en) * | 1991-10-04 | 1994-05-31 | The University Of Tennessee Research Corporation | Human growth hormone induced improvement in depressed T4/T8 ratio |
FR2686899B1 (fr) | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
WO1994008611A1 (en) * | 1992-10-22 | 1994-04-28 | Sloan-Kettering Institute For Cancer Research | GROWTH HORMONE FRAGMENT hGH 108-129 |
EP1281770B1 (en) | 1993-03-10 | 2010-03-03 | GlaxoSmithKline LLC | Human brain phosphodiesterase and screening method |
FR2738842B1 (fr) * | 1995-09-15 | 1997-10-31 | Rhone Poulenc Rorer Sa | Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique |
GB9526733D0 (en) * | 1995-12-30 | 1996-02-28 | Delta Biotechnology Ltd | Fusion proteins |
US5760187A (en) * | 1996-02-22 | 1998-06-02 | Mitsui Toatsu Chemicals, Inc. | Purification process of a human growth hormone |
JP3794748B2 (ja) * | 1996-03-04 | 2006-07-12 | 第一アスビオファーマ株式会社 | メタノール代謝系を有する微生物の培養法 |
US6503729B1 (en) | 1996-08-22 | 2003-01-07 | The Board Of Trustees Of The University Of Illinois | Selected polynucleotide and polypeptide sequences of the methanogenic archaeon, methanococcus jannashii |
MX9605082A (es) * | 1996-10-24 | 1998-04-30 | Univ Autonoma De Nuevo Leon | Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano. |
JP2001510989A (ja) * | 1996-11-01 | 2001-08-07 | スミスクライン・ビーチャム・コーポレイション | 新規コーディング配列 |
US6068991A (en) * | 1997-12-16 | 2000-05-30 | Bristol-Myers Squibb Company | High expression Escherichia coli expression vector |
DK1040192T3 (da) | 1997-12-18 | 2006-12-18 | Monsanto Technology Llc | Insekt-resistente transgene planter og fremgangsmåder til forbedring af delta-endotoksin-aktivitet mod insekter |
US6087128A (en) * | 1998-02-12 | 2000-07-11 | Ndsu Research Foundation | DNA encoding an avian E. coli iss |
WO1999050302A1 (en) | 1998-03-31 | 1999-10-07 | Tonghua Gantech Biotechnology Ltd. | Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production |
US6512162B2 (en) | 1998-07-10 | 2003-01-28 | Calgene Llc | Expression of eukaryotic peptides in plant plastids |
US6271444B1 (en) | 1998-07-10 | 2001-08-07 | Calgene Llc | Enhancer elements for increased translation in plant plastids |
DE19940750A1 (de) | 1998-08-28 | 2000-06-21 | Febit Ferrarius Biotech Gmbh | Träger für Analytbestimmungsverfahren und Verfahren zur Herstellung des Trägers |
CA2362939C (en) | 1999-02-19 | 2010-07-27 | Febit Ferrarius Biotechnology Gmbh | Method for producing polymers |
US6946265B1 (en) | 1999-05-12 | 2005-09-20 | Xencor, Inc. | Nucleic acids and proteins with growth hormone activity |
US6187750B1 (en) | 1999-08-25 | 2001-02-13 | Everyoung Technologies, Inc. | Method of hormone treatment for patients with symptoms consistent with multiple sclerosis |
AT408721B (de) | 1999-10-01 | 2002-02-25 | Cistem Biotechnologies Gmbh | Pharmazeutische zusammensetzung enthaltend ein antigen |
GB9924351D0 (en) | 1999-10-14 | 1999-12-15 | Brennan Frank | Immunomodulation methods and compositions |
US6562790B2 (en) | 2000-02-05 | 2003-05-13 | Chein Edmund Y M | Hormone therapy methods and hormone products for abating coronary artery blockage |
EP1276756A4 (en) | 2000-04-12 | 2004-06-09 | Human Genome Sciences Inc | ALBUMIN FUSION PROTEINS |
US6995246B1 (en) * | 2000-10-19 | 2006-02-07 | Akzo Nobel N.V. | Methods for removing suspended particles from soluble protein solutions |
WO2002043709A1 (fr) | 2000-12-01 | 2002-06-06 | Takeda Chemical Industries, Ltd. | Procede de production d'une preparation contenant une substance bioactive |
WO2002046435A2 (en) * | 2000-12-05 | 2002-06-13 | Alexion Pharmaceuticals, Inc. | Engineered plasmids and their use for in situ production of genes |
US20020164712A1 (en) * | 2000-12-11 | 2002-11-07 | Tonghua Gantech Biotechnology Ltd. | Chimeric protein containing an intramolecular chaperone-like sequence |
CA2446739A1 (en) * | 2001-05-25 | 2002-12-05 | Human Genome Sciences, Inc. | Chemokine beta-1 fusion proteins |
US6720538B2 (en) * | 2001-06-18 | 2004-04-13 | Homedics, Inc. | Thermostat variation compensating knob |
SG159381A1 (en) | 2001-10-10 | 2010-03-30 | Novo Nordisk As | Remodeling and glycoconjugation of peptides |
US20030171285A1 (en) * | 2001-11-20 | 2003-09-11 | Finn Rory F. | Chemically-modified human growth hormone conjugates |
ES2545090T3 (es) | 2001-12-21 | 2015-09-08 | Human Genome Sciences, Inc. | Proteínas de fusión de albúmina y GCSF |
WO2003059934A2 (en) | 2001-12-21 | 2003-07-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
DE60332358D1 (de) * | 2002-09-09 | 2010-06-10 | Hanall Pharmaceutical Co Ltd | Protease-resistente modifizierte interferon alpha polypeptide |
TWI281864B (en) * | 2002-11-20 | 2007-06-01 | Pharmacia Corp | N-terminally monopegylated human growth hormone conjugates and process for their preparation |
EP2460530A3 (en) | 2002-12-31 | 2012-08-29 | Althea Technologies, Inc. | Human growth hormone crystals and methods for preparing them |
EP1594530A4 (en) | 2003-01-22 | 2006-10-11 | Human Genome Sciences Inc | HYBRID PROTEINS OF ALBUMIN |
BRPI0407511A (pt) * | 2003-02-19 | 2006-02-14 | Pharmacia Corp | ésteres de polietilenoglicol ativados |
CA2524623A1 (en) * | 2003-05-09 | 2004-11-18 | Pharmexa A/S | Immunogenic human tnf alpha analogues with reduced cytotoxicity and methods of their preparation |
NZ548256A (en) * | 2004-02-02 | 2010-02-26 | Ambrx Inc | Modified human four helical bundle polypeptides and their uses |
CA2558760A1 (en) * | 2004-06-23 | 2006-01-05 | Usv Limited | Chimeric human growth hormone derived from the placenta and pituitary isoform and processes for obtaining said chimera |
US8282921B2 (en) * | 2004-08-02 | 2012-10-09 | Paul Glidden | tRNA synthetase fragments |
US20060024288A1 (en) * | 2004-08-02 | 2006-02-02 | Pfizer Inc. | tRNA synthetase fragments |
US7998930B2 (en) | 2004-11-04 | 2011-08-16 | Hanall Biopharma Co., Ltd. | Modified growth hormones |
WO2006073846A2 (en) | 2004-12-22 | 2006-07-13 | Ambrx, Inc. | Methods for expression and purification of recombinant human growth hormone |
CN103690936A (zh) * | 2004-12-22 | 2014-04-02 | Ambrx公司 | 经修饰的人类生长激素 |
BRPI0519170A8 (pt) | 2004-12-22 | 2018-05-08 | Ambrx Inc | formulações de hormônio de crescimento humano que compreendem um aminoácido não naturalmente codificado |
WO2006121569A2 (en) | 2005-04-08 | 2006-11-16 | Neose Technologies, Inc. | Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants |
US7576053B2 (en) | 2005-06-13 | 2009-08-18 | Rigel Pharmaceuticals, Inc. | Methods and compositions for treating degenerative bone disorders |
US8030271B2 (en) * | 2005-07-05 | 2011-10-04 | Emisphere Technologies, Inc. | Compositions and methods for buccal delivery of human growth hormone |
WO2007019646A1 (en) * | 2005-08-19 | 2007-02-22 | University Of Technology, Sydney | Liver-directed gene therapy |
AU2006315347A1 (en) | 2005-11-16 | 2007-05-24 | Ambrx, Inc. | Methods and compositions comprising non-natural amino acids |
EP1976551A4 (en) * | 2005-12-23 | 2009-12-30 | Altus Pharmaceuticals Inc | COMPOSITIONS COMPRISING COMPLEX PROTEIN CRYSTALS BY POLYCATIONS AND PROCESSING METHOD THEREOF |
DE102006039479A1 (de) | 2006-08-23 | 2008-03-06 | Febit Biotech Gmbh | Programmierbare Oligonukleotidsynthese |
EP2120869A2 (en) | 2006-12-18 | 2009-11-25 | Altus Pharmaceuticals Inc. | Human growth hormone formulations |
US20080260820A1 (en) * | 2007-04-19 | 2008-10-23 | Gilles Borrelly | Oral dosage formulations of protease-resistant polypeptides |
CN101784655A (zh) * | 2007-04-27 | 2010-07-21 | 菲尼克斯股份有限公司 | 可溶性重组二十面体病毒样颗粒的改良生成和体内装配 |
CN102016045A (zh) | 2007-05-02 | 2011-04-13 | 梅瑞尔有限公司 | 具有改善的表达和稳定性的dna质粒 |
AU2008275911A1 (en) * | 2007-07-19 | 2009-01-22 | Arizona Board of Regents, a body corporate acting for and on behalf of Arizona State University | Self- anchoring MEMS intrafascicular neural electrode |
US7939447B2 (en) * | 2007-10-26 | 2011-05-10 | Asm America, Inc. | Inhibitors for selective deposition of silicon containing films |
CN103694337B (zh) | 2008-02-08 | 2016-03-02 | Ambrx公司 | 经修饰瘦素多肽和其用途 |
SI3050576T1 (sl) | 2008-04-29 | 2021-08-31 | Ascendis Pharma Endocrinology Division A/S | Spojine pegiliranega rekombinantnega humanega rastnega hormona |
US20110020924A1 (en) * | 2008-04-30 | 2011-01-27 | Gradalis, Inc. | Highly pure plasmid dna preparations |
RU2501565C2 (ru) | 2008-06-25 | 2013-12-20 | Брааш Биотех Ллк | Слитый белок дефектная хлорамфеникол ацетилтрансфераза (сат)-соматостатин и его применения |
KR101627879B1 (ko) * | 2008-06-25 | 2016-06-07 | 브라아쉬 바이오테크 엘엘씨 | 강화된 소마토스타틴 면역성을 위한 조성물 및 방법 |
WO2010091122A1 (en) | 2009-02-03 | 2010-08-12 | Amunix, Inc. | Extended recombinant polypeptides and compositions comprising same |
US8703717B2 (en) | 2009-02-03 | 2014-04-22 | Amunix Operating Inc. | Growth hormone polypeptides and methods of making and using same |
WO2010096394A2 (en) | 2009-02-17 | 2010-08-26 | Redwood Biosciences, Inc. | Aldehyde-tagged protein-based drug carriers and methods of use |
US20100216648A1 (en) | 2009-02-20 | 2010-08-26 | Febit Holding Gmbh | Synthesis of sequence-verified nucleic acids |
US9849188B2 (en) | 2009-06-08 | 2017-12-26 | Amunix Operating Inc. | Growth hormone polypeptides and methods of making and using same |
US20120263701A1 (en) | 2009-08-24 | 2012-10-18 | Volker Schellenberger | Coagulation factor vii compositions and methods of making and using same |
CN104017063A (zh) | 2009-12-21 | 2014-09-03 | Ambrx公司 | 经过修饰的猪促生长素多肽和其用途 |
MX349301B (es) | 2009-12-21 | 2017-07-21 | Ambrx Inc | Polipéptidos de somatotropina bovina modificados y sus usos. |
US20110237509A1 (en) | 2010-02-17 | 2011-09-29 | Elona Biotechnologies | hGH AND METHODS FOR PREPARING hGH |
EP2446898A1 (en) | 2010-09-30 | 2012-05-02 | Laboratorios Del. Dr. Esteve, S.A. | Use of growth hormone to enhance the immune response in immunosuppressed patients |
CA2824143C (en) | 2011-01-14 | 2018-12-18 | Redwood Bioscience, Inc. | Aldehyde-tagged immunoglobulin polypeptides and method of use thereof |
RU2473556C1 (ru) * | 2011-07-14 | 2013-01-27 | Закрытое акционерное общество "ГЕНЕРИУМ" | Промышленный способ получения и очистки рекомбинантного гормона роста человека из телец включения |
CN104428009A (zh) | 2012-02-07 | 2015-03-18 | 全球生物疗法美国有限公司 | 核酸输送的区室化方法及其组合物和应用 |
LT2814840T (lt) | 2012-02-15 | 2020-02-25 | Bioverativ Therapeutics Inc. | Faktoriaus viii kompozicijos ir jų gavimo ir naudojimo būdai |
EP2822577B1 (en) | 2012-02-15 | 2019-02-06 | Bioverativ Therapeutics Inc. | Recombinant factor viii proteins |
JP2015513904A (ja) | 2012-03-26 | 2015-05-18 | プロニュートリア・インコーポレイテッドPronutria, Inc. | 栄養断片、タンパク質、および方法 |
CN104470946A (zh) | 2012-03-26 | 2015-03-25 | 普罗努塔利亚公司 | 带电荷营养性蛋白质和方法 |
EP2831102A4 (en) | 2012-03-26 | 2015-12-02 | Pronutria Inc | NUTRIENT FRAGMENTS, NUTRIENT PROTEINS AND METHODS |
JP2015518470A (ja) | 2012-03-26 | 2015-07-02 | プロニュートリア・インコーポレイテッドPronutria, Inc. | 栄養タンパク質および方法 |
US9457096B2 (en) | 2012-07-06 | 2016-10-04 | Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Concet) | Protozoan variant-specific surface proteins (VSP) as carriers for oral drug delivery |
MX2016001667A (es) | 2013-08-08 | 2016-11-07 | Global Bio Therapeutics Inc | Dispositivo de sujecion para procedimientos minimamente invasivos y usos del mismo. |
EP3033097B1 (en) | 2013-08-14 | 2021-03-10 | Bioverativ Therapeutics Inc. | Factor viii-xten fusions and uses thereof |
WO2015048334A2 (en) | 2013-09-25 | 2015-04-02 | Pronutria, Inc. | Edible species nutritive polypeptides and methods of production and use thereof |
EP3988083A1 (en) | 2014-11-21 | 2022-04-27 | Ascendis Pharma Endocrinology Division A/S | Long-acting growth hormone dosage forms |
AU2016301303B2 (en) | 2015-08-03 | 2021-10-07 | Bioverativ Therapeutics Inc. | Factor IX fusion proteins and methods of making and using same |
KR20180135458A (ko) | 2016-04-26 | 2018-12-20 | 알.피.쉐러 테크놀러지즈 엘엘씨 | 항체 컨쥬게이트 및 이의 제조 및 이용 방법 |
AU2019270184A1 (en) | 2018-05-18 | 2020-11-26 | Bioverativ Therapeutics Inc. | Methods of treating hemophilia A |
EP3811962A4 (en) | 2018-06-25 | 2022-03-16 | JCR Pharmaceuticals Co., Ltd. | PROTEIN AQUEOUS LIQUID FORMULATION |
CN109486847B (zh) * | 2018-12-17 | 2021-03-02 | 江南大学 | 基于人工串联启动子的枯草芽孢杆菌高效诱导表达*** |
Family Cites Families (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3853832A (en) * | 1971-04-27 | 1974-12-10 | Harmone Res Foundation | Synthetic human pituitary growth hormone and method of producing it |
US3853833A (en) * | 1971-04-27 | 1974-12-10 | Hormone Res Foundation | Synthetic human growth-promoting and lactogenic hormones and method of producing same |
GB1521032A (en) * | 1974-08-08 | 1978-08-09 | Ici Ltd | Biological treatment |
US4237224A (en) * | 1974-11-04 | 1980-12-02 | Board Of Trustees Of The Leland Stanford Jr. University | Process for producing biologically functional molecular chimeras |
NL7607683A (nl) * | 1976-07-12 | 1978-01-16 | Akzo Nv | Werkwijze ter bereiding van nieuwe peptiden en peptide-derivaten en de toepassing hiervan. |
US4190495A (en) * | 1976-09-27 | 1980-02-26 | Research Corporation | Modified microorganisms and method of preparing and using same |
NZ187300A (en) * | 1977-05-27 | 1982-08-17 | Univ California | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
US4363877B1 (en) * | 1977-09-23 | 1998-05-26 | Univ California | Recombinant dna transfer vectors |
CH630089A5 (de) * | 1977-09-09 | 1982-05-28 | Ciba Geigy Ag | Verfahren zur herstellung von siliciummodifizierten imidyl-phthalsaeurederivaten. |
JPS5449837A (en) * | 1977-09-19 | 1979-04-19 | Kobashi Kogyo Kk | Safety apparatus of soil block making machine |
US4407948A (en) * | 1977-09-23 | 1983-10-04 | The Regents Of The University Of California | Purification of nucleotide sequences suitable for expression in bacteria |
ZA782933B (en) * | 1977-09-23 | 1979-05-30 | Univ California | Purification of nucleotide sequences suitable for expression in bacteria |
US4321365A (en) * | 1977-10-19 | 1982-03-23 | Research Corporation | Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules |
US4366246A (en) * | 1977-11-08 | 1982-12-28 | Genentech, Inc. | Method for microbial polypeptide expression |
EP0001929B1 (en) * | 1977-11-08 | 1989-04-19 | Genentech, Inc. | Plasmid for transforming bacterial host to render it capable of polypeptide expression |
JPS54145289A (en) | 1977-11-08 | 1979-11-13 | Genentech Inc | Improving method of microbiological polypeptide displaying method and means |
BR7807288A (pt) * | 1977-11-08 | 1979-06-12 | Genentech Inc | Processo para sintese de polinucleotidos |
US4356270A (en) * | 1977-11-08 | 1982-10-26 | Genentech, Inc. | Recombinant DNA cloning vehicle |
CA1201668A (en) * | 1977-11-08 | 1986-03-11 | Genentech, Inc. | Synthetic dna and method therefor |
US4652525A (en) * | 1978-04-19 | 1987-03-24 | The Regents Of The University Of California | Recombinant bacterial plasmids containing the coding sequences of insulin genes |
US4411994A (en) * | 1978-06-08 | 1983-10-25 | The President And Fellows Of Harvard College | Protein synthesis |
US4565785A (en) * | 1978-06-08 | 1986-01-21 | The President And Fellows Of Harvard College | Recombinant DNA molecule |
FI792481A (fi) * | 1978-08-11 | 1980-02-12 | Univ California | Syntes av enkaroytiskt protein genom anvaendning av mikro-organismer |
EP0009930B2 (en) * | 1978-10-10 | 1988-08-24 | The Board Of Trustees Of The Leland Stanford Junior University | Recombinant dna, method for preparing it and production of foreign proteins by unicellular hosts containing it |
US4332892A (en) * | 1979-01-15 | 1982-06-01 | President And Fellows Of Harvard College | Protein synthesis |
IE52036B1 (en) * | 1979-05-24 | 1987-05-27 | Univ California | Non-passageable viruses |
ZA802992B (en) * | 1979-06-01 | 1981-10-28 | Univ California | Human pre-growth hormone |
US4898830A (en) * | 1979-07-05 | 1990-02-06 | Genentech, Inc. | Human growth hormone DNA |
US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
GR70279B (ko) * | 1979-09-12 | 1982-09-03 | Univ California | |
IL59690A (en) * | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
GR79124B (ko) * | 1982-12-22 | 1984-10-02 | Genentech Inc | |
US4859600A (en) * | 1983-04-25 | 1989-08-22 | Genentech, Inc. | Recombinant procaryotic cell containing correctly processed human growth hormone |
CA1267615A (en) * | 1984-08-27 | 1990-04-10 | Dan Hadary | Method for recovering purified growth hormones from genetically engineered microorganisms |
-
1979
- 1979-07-05 US US06/055,126 patent/US4342832A/en not_active Expired - Lifetime
-
1980
- 1980-06-12 IE IE2194/84A patent/IE50462B1/en not_active IP Right Cessation
- 1980-06-12 IE IE1214/80A patent/IE50460B1/en not_active IP Right Cessation
- 1980-06-12 IE IE2193/84A patent/IE50461B1/en unknown
- 1980-06-13 NZ NZ201312A patent/NZ201312A/xx unknown
- 1980-06-13 NZ NZ194043A patent/NZ194043A/xx unknown
- 1980-06-15 IL IL69492A patent/IL69492A/xx unknown
- 1980-06-15 IL IL60312A patent/IL60312A/xx unknown
- 1980-06-17 ZA ZA00803600A patent/ZA803600B/xx unknown
- 1980-06-19 CA CA000354359A patent/CA1164375A/en not_active Expired
- 1980-06-20 ZW ZW141/80A patent/ZW14180A1/xx unknown
- 1980-06-20 AU AU59498/80A patent/AU533697B2/en not_active Expired
- 1980-06-23 MX MX026650A patent/MX172674B/es unknown
- 1980-06-23 YU YU01635/80A patent/YU163580A/xx unknown
- 1980-06-24 DE DE3050722A patent/DE3050722C2/de not_active Expired
- 1980-06-24 DE DE3050725A patent/DE3050725C2/de not_active Expired
- 1980-06-24 DE DE3023627A patent/DE3023627A1/de not_active Ceased
- 1980-06-25 FR FR8014108A patent/FR2460330B1/fr not_active Expired
- 1980-06-25 FI FI802030A patent/FI802030A/fi not_active Application Discontinuation
- 1980-06-26 BE BE1/9864A patent/BE884012A/fr not_active IP Right Cessation
- 1980-06-26 IT IT23070/80A patent/IT1131393B/it active Protection Beyond IP Right Term
- 1980-06-27 CH CH4958/80A patent/CH661939A5/de not_active IP Right Cessation
- 1980-07-01 PL PL1980225376A patent/PL149278B1/pl unknown
- 1980-07-01 OA OA57150A patent/OA06562A/xx unknown
- 1980-07-01 BG BG8048346A patent/BG41135A3/xx unknown
- 1980-07-01 AT AT80103748T patent/ATE82324T1/de not_active IP Right Cessation
- 1980-07-01 EP EP80103748A patent/EP0022242B1/en not_active Expired - Lifetime
- 1980-07-02 PT PT71487A patent/PT71487A/pt unknown
- 1980-07-02 EG EG80392A patent/EG14819A/xx active
- 1980-07-02 GR GR62345A patent/GR69320B/el unknown
- 1980-07-03 GB GB08234691A patent/GB2121047B/en not_active Expired
- 1980-07-03 WO PCT/US1980/000838 patent/WO1981000114A1/en unknown
- 1980-07-03 PH PH24236A patent/PH19814A/en unknown
- 1980-07-03 BR BR8008736A patent/BR8008736A/pt unknown
- 1980-07-03 GB GB8021860A patent/GB2055382B/en not_active Expired
- 1980-07-04 KR KR1019800002658A patent/KR830003574A/ko unknown
- 1980-07-04 JP JP55092161A patent/JPH0612996B2/ja not_active Expired - Lifetime
- 1980-07-04 DD DD80244149A patent/DD210071A5/de unknown
- 1980-07-04 ES ES493149A patent/ES493149A0/es active Granted
- 1980-07-04 AR AR80281658A patent/AR244341A1/es active
- 1980-07-04 DD DD80244147A patent/DD210070A5/de unknown
- 1980-07-04 CS CS804809A patent/CS250652B2/cs unknown
- 1980-07-04 DD DD80222413A patent/DD157343A5/de unknown
-
1981
- 1981-02-02 ES ES499043A patent/ES8205265A1/es not_active Expired
- 1981-02-20 NO NO81810608A patent/NO167673C/no unknown
- 1981-03-04 DK DK198100973A patent/DK173503B1/da not_active IP Right Cessation
- 1981-03-05 RO RO103596A patent/RO93374B/ro unknown
- 1981-08-26 US US06/296,099 patent/US4634677A/en not_active Expired - Lifetime
-
1982
- 1982-03-09 US US06/356,564 patent/US4601980A/en not_active Expired - Lifetime
- 1982-03-09 US US06/361,160 patent/US4604359A/en not_active Expired - Lifetime
- 1982-05-12 CS CS823457A patent/CS250655B2/cs unknown
-
1983
- 1983-01-17 FR FR8300612A patent/FR2518572B1/fr not_active Expired
- 1983-04-25 CA CA000426675A patent/CA1202256A/en not_active Expired
- 1983-06-01 YU YU01212/83A patent/YU121283A/xx unknown
- 1983-06-01 YU YU01211/83A patent/YU121183A/xx unknown
- 1983-08-14 IL IL69492A patent/IL69492A0/xx unknown
- 1983-08-24 AU AU18388/83A patent/AU1838883A/en not_active Abandoned
-
1984
- 1984-04-09 CS CS842708A patent/CS254973B2/cs unknown
- 1984-08-13 SG SG569/84A patent/SG56984G/en unknown
- 1984-09-13 KE KE3450A patent/KE3450A/xx unknown
- 1984-09-13 KE KE3451A patent/KE3451A/xx unknown
- 1984-09-13 KE KE3446A patent/KE3446A/xx unknown
- 1984-09-25 US US06/654,340 patent/US4658021A/en not_active Expired - Lifetime
- 1984-11-08 HK HK874/84A patent/HK87484A/xx not_active IP Right Cessation
- 1984-11-08 HK HK875/84A patent/HK87584A/xx not_active IP Right Cessation
- 1984-11-08 HK HK873/84A patent/HK87384A/xx not_active IP Right Cessation
-
1985
- 1985-01-16 FI FI850198A patent/FI850198A0/fi not_active Application Discontinuation
- 1985-12-30 MY MY763/85A patent/MY8500763A/xx unknown
- 1985-12-30 MY MY765/85A patent/MY8500765A/xx unknown
- 1985-12-30 MY MY764/85A patent/MY8500764A/xx unknown
-
1986
- 1986-02-24 NO NO86860680A patent/NO167674C/no unknown
- 1986-11-12 KR KR1019860009542A patent/KR870000701B1/ko not_active IP Right Cessation
-
1989
- 1989-04-18 JP JP1099888A patent/JPH0648987B2/ja not_active Expired - Lifetime
-
1990
- 1990-10-18 DK DK251490A patent/DK172132B1/da not_active IP Right Cessation
-
1992
- 1992-09-25 JP JP4256344A patent/JP2622479B2/ja not_active Expired - Lifetime
-
1993
- 1993-06-29 NL NL930114C patent/NL930114I2/nl unknown
-
1994
- 1994-05-27 US US08/250,639 patent/US5424199A/en not_active Expired - Fee Related
-
1995
- 1995-06-01 US US08/457,282 patent/US5795745A/en not_active Expired - Fee Related
- 1995-11-29 JP JP7310696A patent/JPH08242881A/ja active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR830003574A (ko) | 변칙합성 유전자의 미생물을 통한 발현 | |
Buck et al. | Upstream activator sequences are present in the promoters of nitrogen fixation genes | |
IL71906A (en) | Method for producing a baculovirus vector capable of producing a selected non-fused recombinant protein or capable of being utilized as a transfer vector;a recombinant baculovirus expression vector capable of expressing under the control of a baculovirus polyhedrin promoter a selected non-fused protein in a host insect cell;a recombinant transfer vector utilizing a baculovirus polyhedrin promoter and flanking sequences for introducing a selected gene into a baculovirus genome,or for insertion of a selected gene for a genetic manipulation of a baculovirus;and a method for the synthesis of a selected non-fused protein in a host insect cell using the expression vector | |
KR880014110A (ko) | 인체 프로아포지방단백질 a-i의 발현 | |
KR840002031A (ko) | Dna, 재조합 dna, 이들을 갖는 숙주 및 폴리펩타이드를 제조하는 방법 | |
CA2090549C (en) | Bacterial surface protein expression | |
KR950703642A (ko) | 만누로난 씨(c)-5-에피머라제를 암호화하는 서열을 포함하는 디 엔 에이(dna) 화합물(dna compounds comprising sequences encoding mannuronan c-5-epimerase) | |
KR870700095A (ko) | AraB 프로모터 및 미생물학적 방법에 의해 세크로핀을 포함한 폴리펩타이드를 제조하는 방법 | |
Kuhlemeier et al. | Cloning of nitrate reductase genes from the cyanobacterium Anacystis nidulans | |
JPH1084978A (ja) | 改良されたリボフラビン生産 | |
ATE542913T1 (de) | Promotorvarianten zur expression von genen in einer pilzzelle | |
CN117701548A (zh) | 一种檀香烯合成酶突变体以及应用 | |
KR970001542A (ko) | 엔도글리코세라미데이즈 유전자 | |
WO1994000463A3 (en) | Production of hyaluronic acid by transeformed microorganisms | |
Sharp et al. | Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster | |
US5281531A (en) | Host and vector for producing D-ribose | |
KR970027314A (ko) | 악티노마이세트 프로모터 | |
JPS58500590A (ja) | 枯草菌におけるクロ−ン化異種遺伝子生産物の制御された蓄積のための方法およびベクタ− | |
KR930702528A (ko) | 스트렙토마이세스 리비단으로부터 분리하고 정제한 단백질 가수분해 효소 x를 암호화하는 dna 시퀸스 | |
WO1989009824A3 (en) | Gene synthesis | |
KR850004275A (ko) | 프로모터 및 그의 이용 | |
JPH06277073A (ja) | 蛋白質のトランスロケーションマシナリーをコードする遺伝子dna | |
KR100455004B1 (ko) | 신규 프로모터 및 이를 이용한 유전자 발현방법 | |
KR920004577A (ko) | 단백질 합성의 cks 방법 | |
CA2224777A1 (en) | New immunoregulatory protein lst-1 |