JPH03102245A - Absorbancy type automatic analyser - Google Patents
Absorbancy type automatic analyserInfo
- Publication number
- JPH03102245A JPH03102245A JP1239144A JP23914489A JPH03102245A JP H03102245 A JPH03102245 A JP H03102245A JP 1239144 A JP1239144 A JP 1239144A JP 23914489 A JP23914489 A JP 23914489A JP H03102245 A JPH03102245 A JP H03102245A
- Authority
- JP
- Japan
- Prior art keywords
- absorbance
- absorbancy
- wavelength
- reaction
- difference
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000006243 chemical reaction Methods 0.000 claims abstract description 74
- 238000004458 analytical method Methods 0.000 claims abstract description 30
- 238000002835 absorbance Methods 0.000 claims description 53
- 238000003860 storage Methods 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 23
- 239000000243 solution Substances 0.000 abstract description 15
- 238000000034 method Methods 0.000 abstract description 13
- 238000010521 absorption reaction Methods 0.000 abstract description 11
- 238000002347 injection Methods 0.000 abstract description 10
- 239000007924 injection Substances 0.000 abstract description 10
- 239000012490 blank solution Substances 0.000 abstract description 7
- 238000012937 correction Methods 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 26
- 230000003287 optical effect Effects 0.000 description 23
- 239000007788 liquid Substances 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/274—Calibration, base line adjustment, drift correction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
Landscapes
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、複数の反応セルを搬送体に配列させ、試料注
入ステーション、試薬注入ステーション、測定ステーシ
ョンに移動させ、試料と反応試薬の混合液の吸光度から
目的戒分の濃度を測定するようにした自動分析装置にお
ける、前記反応セルの寸法不均一による混合液の光路長
を補正する技術に間する．
（従来技術）
反応セルに収容ざれた試料と試薬の混合液（以下、試料
反応液という）の吸光度から目的戊分の濃度を測定する
分析装置においでは、吸光度か反応せルの光路長に大き
く左右ざれるため、光路長か均一となるように反応セル
を高い精度で製作する必要かあるが、コストとの兼合で
或る程度の誤差を許容せざるを得ない．
このような反応セルの寸法誤差に起因する分析誤差を補
正するため、反応セルに標準液体を収容し、これに近赤
外波長域の波長の内、共に反応セルの材質にはあまり吸
収を受けない波長で、一方は標準液体により吸収を受け
る波長λ，と、他方は標準液体による吸収を受けない波
長λ２とを用いて吸光度を測定し、この吸光度差に基づ
いて反応セルの光路長を求めるようにした自動分析装置
が提案されている（特開昭６０−１８３５６０号公報）
．（発明が解決しようとする問題点）
しかしながら、現在普及している自動分析装置において
は紮外域から可視域の波長での吸光度の測定を可能とす
る吸光度計だけを具備しでおり、前記従来技術を適用す
るため｛こは、ざらに特別な近赤外領域での吸光度の測
定が可能な第２の吸弄度計を用意する必要があるという
問題がある．本発明はこのような問題に鑑みでなざれた
ものであって、その目的とするところは、近赤外領域の
吸光度計を必要としないばかりでなく、分析波長に可及
的に近い波長を用いて反応セルの光路長を正確に補正す
ることにより、信頼性の高い精度の分析結果を得ること
がてきる自動分析装置ヲ褪供ずることにある．
（課題を解決するための手段）
このような問題を解消するために本発明においては、等
吸収波長と分析波長における吸光度を選択的１こ測定す
る手段と、基準となる反応セルの試液ブランク液の等吸
収波長における吸光度を記憶する記憶手段と、試料反応
液の等吸収波長｛こおける吸光度と前記記憶手段の値と
の比を求め、前記比と分析波長での試料の吸光度との積
を演算する手段を備えるようにした．
（作用）
分析波長に近い領域の等吸収波長における吸光度を用い
るため、反応セルの光路長を正確に補正することが可能
となる．
（実施例）
そこで、以下に本発明の詳細を図示した実施例に基づい
て説明する．
第１図は本発明の一実施例を示すものであって、図中行
号１は、反応テーブルで、容器と測定セルを兼ねた反応
セル２、２、２・・・・を列状に収容して、反応セル２
を試料注入ステーション３、試薬注入ステーション４、
後述ｊる測定ステーション５、及び洗浄ステーションに
循環的ｆこ移送するように構Ｆ＆ざれている．
５は、前述の測定ステーションで、反応セルの移１７］
経路を挟んで一方の側には光源ランプ６を、また他方の
側には囲折フィルター等からなる分光器７と、祷数の光
電変換素子８、８、８・・・・を設けてなる受光部を配
通して、所望波長の光を選択的に検出する多波長光度計
により構或されている．
多波長光度計の各光電変換素子８、８、８・・・・から
の信号は、マルチブレクサ等の信号切換器９を介して対
数変換回路１０、アナログーディジタル変換器１１に入
力し、ディジタル信号に変換ざれて後述する演算制御装
置１２に入力しでいる。Detailed Description of the Invention (Industrial Application Field) The present invention involves arranging a plurality of reaction cells on a carrier, moving them to a sample injection station, a reagent injection station, and a measurement station, and discharging a mixture of a sample and a reaction reagent. In this paper, we develop a technique for correcting the optical path length of a liquid mixture due to nonuniform dimensions of the reaction cell in an automatic analyzer that measures the concentration of a target compound from the absorbance of the reaction cell. (Prior art) In an analyzer that measures the concentration of a target substance from the absorbance of a mixed solution of sample and reagent (hereinafter referred to as sample reaction solution) housed in a reaction cell, there is a large difference between the absorbance and the optical path length of the reaction cell. Since the left and right deviations occur, it is necessary to manufacture the reaction cell with high precision so that the optical path length is uniform, but a certain degree of error must be allowed in consideration of cost. In order to correct analytical errors caused by such dimensional errors in the reaction cell, a standard liquid is contained in the reaction cell, and a standard liquid is added to the liquid at wavelengths in the near-infrared wavelength range, which are not well absorbed by the material of the reaction cell. The absorbance is measured using two wavelengths, one wavelength λ, which is absorbed by the standard liquid, and the other wavelength λ2, which is not absorbed by the standard liquid, and the optical path length of the reaction cell is determined based on this absorbance difference. An automatic analyzer has been proposed (Japanese Unexamined Patent Publication No. 183560/1983).
．． (Problems to be Solved by the Invention) However, the currently widespread automatic analyzers are only equipped with an absorbance meter that can measure absorbance at wavelengths from the ultraviolet region to the visible region. In order to apply this, there is a problem in that it is necessary to prepare a second absorbance meter that can measure absorbance in a special near-infrared region. The present invention was developed in view of these problems, and its purpose is not only to eliminate the need for an absorbance meter in the near-infrared region, but also to use a wavelength as close as possible to the analysis wavelength. The purpose of the present invention is to provide an automatic analyzer that can obtain highly reliable and accurate analysis results by accurately correcting the optical path length of the reaction cell. (Means for Solving the Problems) In order to solve these problems, the present invention provides a means for selectively measuring the absorbance at the isosbestic wavelength and the analysis wavelength, and a method for selectively measuring the absorbance at the isoabsorption wavelength and the analysis wavelength, and a reagent blank solution of the reaction cell as a reference. A storage means for storing the absorbance at the isoabsorption wavelength of the sample reaction solution, and a ratio between the absorbance at the isoabsorption wavelength of the sample reaction solution and the value in the storage means, and the product of the ratio and the absorbance of the sample at the analysis wavelength. A means of calculation was provided. (Function) Since the absorbance at the isosbestic wavelength in the region close to the analysis wavelength is used, it is possible to accurately correct the optical path length of the reaction cell. (Example) Therefore, the details of the present invention will be explained below based on an illustrated example. FIG. 1 shows an embodiment of the present invention, and row number 1 in the figure is a reaction table, in which reaction cells 2, 2, 2, etc., which also serve as containers and measurement cells are housed in a row. Then, reaction cell 2
sample injection station 3, reagent injection station 4,
It is configured to cyclically transfer the water to a measuring station 5 and a cleaning station, which will be described later. 5 is the measurement station mentioned above, and the reaction cell is moved 17]
A light source lamp 6 is provided on one side of the path, and a spectrometer 7 consisting of a surrounding filter, etc., and an arbitrary number of photoelectric conversion elements 8, 8, 8, etc. are provided on the other side. It consists of a multi-wavelength photometer that selectively detects light of a desired wavelength through a light receiving section. Signals from each photoelectric conversion element 8, 8, 8, . The data has been converted into and input to the arithmetic and control unit 12, which will be described later.
演算制御装置１２は、マイクロプロセッサ１３と、分析
作業のシーケシスを格納した記憶回路１４と、試液ブラ
ンク液の等吸収波長λ１における吸光度Ａλｌｂを格納
する第１の記憶回路１５と、２つの分析波長久，、λ２
における吸光度Ａλ１１１％ Ａλ２ｂの差Ａ入＋ｂ−
Ａ入，ｂを格納する第２の記憶回路１６から構成ざれて
いる．次に、このように構成ざれた装置の動作をキシリ
ジプルー法を用いたマグネシュウムの分析（こ例を採っ
て第２図１こ示した吸収曲線に基づいて説明する．
マグネシュウム検出試薬であるキシリジフル反応試薬と
、試料量と同一量の蒸留＊等の純水を混合してなる試液
ブランク液を反応セル２に収容して、信号切換器９によ
り試薬のＪ度だけに影響を受ける波長（今の例では５６
３ナノメートル）、いわゆる′４吸収波長久１における
吸光度八λ１ｂと、２つの分析波長人＋、入２（今の例
では６００ナノメートルと７００ナノメートル）での吸
光度Ａ入＋ｂ、Ａλ２ゎを測定する．これら測定した複
数の吸光慶Ａ入，ｂの平均値Ａ入１を第１の記憶回路１
５に、また分析波長λ１、λ２における吸光度の差分Ａ
入，。一Ａλ２ｂの平均値Ａ入．／λ２ｂ’ｔ求めて第
２の記憶回路１６に格納する．
次に、予め目的或分の濃度Ｃ ｓ ｔが既知である標準
試料と所定量の反応試薬を反応セル２に注入して標準試
料反応液を調製し、この標準試料反応液の等吸収波長λ
ｌにおける吸光度Ａλ１．、分析波長大，、入２におけ
る吸光度Ａλ１３％ Ａλ２，を測定する．この吸光度
Ａλ，！、Ａλ２，によりその差分Ａλ１／入，，＝Ａ
入，，−Ａλ２，を求める．一方、Ｍ１の記憶回路１５
に格納されている試液フランク液の等吸収波長における
吸光度の平均値Ａλｔｂと、今測定した標準試料反応液
の等吸収波長における吸光度Ａλ五．との比Ａλ＋−／
Ａλ！ゎ＝Ｌを求め、さらに標準試料反応液の２つの分
析波長間の吸光度差Ａλ，／λ２３と比Ｌとの積ＬＸＡ
λ，／λ２．を求める．
ところで、この比しは、基準となる反応セル２の光路差
との比を表すことになるから、ＬＸＡλ，／λ２ｓは、
反応セルの光路差が補正ざれた値となる．
ついで、換算係数Ｋ＝Ｃ．．／（Ａλ，／λ２，ーＡλ
１。）を求め、この＠Ｋを記憶させてお〈．このような
準備を終えた段階で、試料注入ステーション３において
所定量の試料を、また試薬注入ステーション４において
所定量の反応試薬を注入して試料反応液を調製する．反
応セル２が測定ステーション５に到達した段階で、演算
制御装Ｉ！１２は、信号切換器９により等吸収波長Ａλ
１、及び分析波長Ａλ，、Ａλ２における吸光度信号を
選択する．
演算制御装１ｌ１２は、等吸収波＆Ａλｈと第１の記憶
回路１５に格納ざれでいる吸光度Ａλｌｂと？比Ｌ．＝
Ａλ１ｋ／Ａ入１ｆｉを求め、この比Ｌｈと、分析波長
Ａλ■、Ａλ２ｋの吸光度の差分Ａ入，／λ■：Ａλ，
ｋ− Ａλ２，との積ＬｋｘＡλ，／λ２ｋ％演算し、
今、分析に使用した反応でル２の光路差に起因する誤差
を補正した値を出力する．
この補正後のｉｔ−ｉｔ ＸＡλ，／λ２，と試液ブラ
ンク液の分析波長における２つの差分Ａλ，／λ２，と
の差Ｌｋ ＸＡλｌ／λ，，−Ａλ，／λ２。を求めこ
れに濃度換算係数Ｋを乗じた（Ｌｋ ＸＡλＩ／λ２，
−ＡλＩ／入ｚｂ）ＸＫｔ演算して目的或分の濃度を算
出する．これにより目的成分の濃度を、反応セル２の光
路長変動による誤差を補正した値を得ることができる．
もとより等吸収波長Ａ入ｌが分析波長Ａλ１、Ａ入２に
援近した波＆域にあるため、等吸収波長Ａ入）における
吸光度は分析時の光路差を正確に表すことになり、精度
の高い光路差補正が可能となる．
［比較例］
或る吸光度式自動分析装置の２０個の反応セルのそれぞ
れに、同一のキシリジブルー法の試液ブランク液を分注
して８吸収波長５６３ナノメートルにおける吸光度Ａ入
ＩＩ，（表１における工）、２つの分析波長（６００ｎ
ｍと７００ｎｍ）における吸光度差Ａλｌ／′λ２ｔ，
（表１１こおけるＩＩ）、及び表１における■の欄のデ
ータの平均値Ａλ１／入ｚｋ＝ １．２１４７と各反応
セルの′：４吸収波長での吸光度とにより反応セルの光
路差を補正した値（表１における■）とをそれぞれ求め
たところ表１のような結果となった．
表１
表２
一方、萌述の反応セルを使用するとともに、これら反応
セルに標準試料と反応試薬を混合調製した同一の標準試
料反応液を用いで、等吸収波長における吸光度（表２に
おける■）、２つの分析波長（６００ｎｍと７００ｎｍ
）における吸光度差（表２における■）、各試料の吸光
度差と、２つの分析波長（６００ｎｍと７００ｎｍ）に
おける試液ブランク液の吸光度差との差分（表２におけ
るＩＩＩ）、表２のＨの各測定＠を表１の工等吸収の平
均値（１．１２８０）に基づいて反応セルの光路差を補
正した分析波長におＣナる吸光度差（表２における■）
、及び表２の■の値と表１の■の平均値（１．２１４８
）との差（表２ＣこおけるＶ）をそれぞれ求めたところ
、表２のようになった．表２の■欄と■欄の値のＣｖ値
をそれぞれ計算したところ、前者、つまり反応セルの光
路差を補正しでいないものはＣＶ＝２．１％となり、ま
た後者、つまり等吸収１こよる反応セルの光路差補正を
行なったものは、Ｃ Ｖ ＝ ０．８３％となった．こ
のことから等吸収波長を用いて反応セルの光路差を補正
する本発明は、分析結果の再現牲を向よさせる上で極め
て有効な手法であることが実証できた。The arithmetic and control unit 12 includes a microprocessor 13, a memory circuit 14 that stores the sequence of analysis work, a first memory circuit 15 that stores the absorbance Aλlb of the test solution blank at the isoabsorption wavelength λ1, and two analysis wavelengths. ,,λ2
Absorbance Aλ111% Aλ2b difference A+b-
It consists of a second memory circuit 16 that stores A and B. Next, the operation of the apparatus configured as described above will be explained based on the analysis of magnesium using the xyridiflu method (taking this as an example, and based on the absorption curve shown in Fig. 2). A reagent blank solution made by mixing the same amount of distilled water or other pure water as the sample amount is placed in the reaction cell 2, and the signal switch 9 selects a wavelength that is affected only by the J degree of the reagent (in this example). So 56
3 nanometers), the absorbance at the so-called '4 absorption wavelength K1, 8λ1b, and the absorbance at the two analytical wavelengths A +b, Aλ2ゎ (600 nm and 700 nm in the present example). Measure. The average value A of these measured absorption values A and b is stored in the first storage circuit 1.
5, and the difference A in absorbance at analysis wavelengths λ1 and λ2
Enter,. - Average value of Aλ2b. /λ2b't and stores it in the second memory circuit 16. Next, a standard sample reaction solution whose target concentration C s t is known in advance and a predetermined amount of reaction reagent are injected into the reaction cell 2 to prepare a standard sample reaction solution, and the equiabsorption wavelength λ of this standard sample reaction solution is
Absorbance at Aλ1. , Measure the absorbance Aλ13% Aλ2 at the analysis wavelength large, input 2. This absorbance Aλ,! , Aλ2, the difference Aλ1/in, ,=A
Find input, , -Aλ2,. On the other hand, the memory circuit 15 of M1
The average value Aλtb of the absorbance at the isoabsorption wavelength of the reagent flank solution stored in , and the absorbance Aλtb at the isoabsorption wavelength of the standard sample reaction solution just measured. The ratio Aλ+-/
Aλ! Calculate ゎ=L, and further calculate the product LXA of the absorbance difference Aλ,/λ23 between the two analysis wavelengths of the standard sample reaction solution and the ratio L.
λ, /λ2. Find. By the way, this ratio represents the ratio to the optical path difference of the reaction cell 2 which is the reference, so LXAλ,/λ2s is
This is the corrected value for the optical path difference of the reaction cell. Then, the conversion coefficient K=C. ．． /(Aλ, /λ2, -Aλ
1. ), memorize this @K, and <. After completing such preparations, a predetermined amount of sample is injected at sample injection station 3, and a predetermined amount of reaction reagent is injected at reagent injection station 4 to prepare a sample reaction solution. When the reaction cell 2 reaches the measurement station 5, the arithmetic and control unit I! 12 is the equiabsorption wavelength Aλ by the signal switcher 9.
1, and the absorbance signals at the analysis wavelengths Aλ, , Aλ2 are selected. The arithmetic and control unit 1l12 outputs the equal absorption wave &Aλh and the absorbance Aλlb stored in the first storage circuit 15. Ratio L. =
Find Aλ1k/A1fi, and calculate the difference between this ratio Lh and the absorbance of the analysis wavelengths Aλ■ and Aλ2k Aλ, /λ■:Aλ,
k-Aλ2, and calculate the product LkxAλ,/λ2k%,
Now, output the value corrected for the error caused by the optical path difference of Le 2 using the reaction used in the analysis. The difference Lk between it-it XAλ, /λ2 after this correction and the two differences Aλ, /λ2 in the analysis wavelength of the reagent blank solution. was calculated and multiplied by the concentration conversion coefficient K (Lk XAλI/λ2,
-AλI/input zb)XKt calculation to calculate the target concentration. Thereby, it is possible to obtain a concentration of the target component with a value corrected for errors due to variations in the optical path length of the reaction cell 2. Of course, since the isoabsorption wavelength A is in the wavelength range close to the analysis wavelengths Aλ1 and A2, the absorbance at the isoabsorption wavelength A is an accurate representation of the optical path difference during analysis, which improves accuracy. High optical path difference correction is possible. [Comparative Example] The same test solution blank solution of the xylidi blue method was dispensed into each of the 20 reaction cells of a certain absorbance-type automatic analyzer, and the absorbance at an absorption wavelength of 563 nanometers was measured as follows: ), two analytical wavelengths (600n
m and 700 nm), the absorbance difference Aλl/'λ2t,
(II in Table 11), and the average value Aλ1/input zk = 1.2147 of the data in the column ■ in Table 1 and the absorbance at the ':4 absorption wavelength of each reaction cell to correct the optical path difference of the reaction cell. When the values (■ in Table 1) were calculated, the results shown in Table 1 were obtained. Table 1 Table 2 On the other hand, when Moejo's reaction cells were used and the same standard sample reaction solution prepared by mixing the standard sample and reaction reagent was used in these reaction cells, the absorbance at the isosbestic wavelength (■ in Table 2) , two analysis wavelengths (600nm and 700nm
) (■ in Table 2), the difference between the absorbance difference of each sample and the absorbance difference of the test solution blank solution at the two analysis wavelengths (600 nm and 700 nm) (III in Table 2), each of H in Table 2 The absorbance difference (■ in Table 2) when the measurement @ is changed to the analysis wavelength corrected for the optical path difference of the reaction cell based on the average value of the optical absorption (1.1280) in Table 1.
, and the average value of ■ in Table 2 and ■ in Table 1 (1.2148
) (Table 2C), the results are as shown in Table 2. When we calculated the Cv values for the values in columns ■ and ■ in Table 2, we found that the former, that is, the one in which the optical path difference of the reaction cell was not corrected, was CV = 2.1%, and the latter, that is, the one in which the optical path difference of the reaction cell was not corrected. When the optical path difference of the reaction cell was corrected according to the method, CV = 0.83%. From this, it has been demonstrated that the present invention, which corrects the optical path difference of the reaction cell using the isoabsorption wavelength, is an extremely effective method for improving the reproducibility of analysis results.
なお、この実施例においてはキシリジブルー法を例に採
って説明したが、試料に含まれる総タンパク量を測定す
るどウレット法に適用しても同様の作用を奏する．
すなわち、ビウレット法試薬にあっては第３図に示した
ように波長７００ナノメートル附近が等吸収波長久よと
なるので、この波長Ａλ１の吸光度Ａλ１と、分析波長
である５４０ナノメートルと、波長７００ナノメートル
とにおける吸光度Ａλ，、Ａλ２を測定すれば、前述と
同様の手法により反応セルの光路差を補正することがで
きることは明らかである．
なお、血清等のように混濁或分を含む試料に対しでは、
混濁成分を含む標準試料と試薬との混合液を用いて検体
ブランク値を求め、これにより等吸収波長における吸光
度を補正するようにすればよい．
また、本発明においては２つの分析波長における吸光度
の差分を用いでいるが、単一の分析波長の吸光度を用い
るものに適用しても同様の作用を奏することは明らかで
ある．
ざらに、この天施例においでは複数の反応セル｛こつい
ての等吸収波長における吸光度、試液ブランク液の平均
値を使用しているが、基準となる１つの反応セルについ
ての値を基準として用いでも同様の作用を奏することは
明らかである．なお、本発明においでは、反応セルの製
造公差に基づく光路差を補正するようＣこしでいるが、
一般的（こ反応試薬の分注量は極めで少ないばかりでな
く、１つの試薬の分注作業が終了した段階で洗浄液によ
り分注具を洗浄する場合《こは、分注具内に洗浄液が残
留し、これによる反応試薬の希釈に起因する各反応セル
への反応試薬の実貢的な分注量のばらつきの補正にも適
用することもできる．
（発明の効果）
以上、説明したように本発明においては、等吸収波長と
分析′Ｓ長における吸光度を選択的に測定する手段と、
基準となる反応セルの試液ブランウ液の等吸収波長にお
ける吸光度を記憶する記憶手段と、試料反応液の等吸収
波長における吸光度と前記記憶手段の値との比を求め、
前記比と分析波長での試料の吸光度との積を演算する手
段ｔ！備えたので、分析波長に近い領域の等吸収波長に
おける吸光度により反応セルの光路長を補正して、吸光
度か大きくで反応セルの光路長の影１ｆを受け易い分析
法に対しでも、信頼性の高い測定結果を得ることかでき
る．Although this example has been explained using the xylidiblue method as an example, the same effect can be achieved even if the method is applied to the uret method for measuring the total amount of protein contained in a sample. In other words, for the biuret method reagent, as shown in Figure 3, the wavelength around 700 nanometers is the equiabsorption wavelength, so the absorbance Aλ1 of this wavelength Aλ1, the analysis wavelength of 540 nanometers, and the wavelength It is clear that by measuring the absorbance Aλ, Aλ2 at 700 nanometers, it is possible to correct the optical path difference in the reaction cell using the same method as described above. In addition, for samples containing turbidity such as serum,
The sample blank value can be determined using a mixture of a standard sample and reagent containing turbid components, and the absorbance at the isosbestic wavelength can be corrected using this value. Furthermore, although the present invention uses the difference in absorbance at two analytical wavelengths, it is clear that the same effect can be achieved even if the present invention is applied to one that uses absorbance at a single analytical wavelength. Generally speaking, in this example, the average value of the absorbance at the equiabsorption wavelength of multiple reaction cells and the sample solution blank solution is used, but the value for one reaction cell is used as the standard. However, it is clear that it has a similar effect. In addition, in the present invention, the optical path difference based on the manufacturing tolerance of the reaction cell is corrected, but
Generally speaking (not only is the amount of reaction reagent dispensed extremely small, but the dispensing tool is cleaned with a cleaning solution after dispensing one reagent) The present invention can also be applied to correction of variations in the actual amount of the reaction reagent dispensed to each reaction cell due to residual dilution of the reaction reagent. (Effects of the Invention) As explained above, In the present invention, a means for selectively measuring absorbance at an isosbestic wavelength and an analysis 'S length;
A storage means for storing the absorbance at the isoabsorption wavelength of the test solution Braun's solution of the reaction cell serving as a reference, and a ratio between the absorbance at the isoabsorption wavelength of the sample reaction solution and the value of the storage means,
means t! for calculating the product of said ratio and the absorbance of the sample at the analysis wavelength; Since the optical path length of the reaction cell is corrected by the absorbance at the isobestic wavelength in the region close to the analysis wavelength, reliability can be improved even for analytical methods that are susceptible to the influence of the optical path length of the reaction cell 1f due to the large absorbance. It is possible to obtain high measurement results.
第１図は本発明の一実施例を示す装置の構成図、第２図
はキシリジブルー法試藁の吸収曲線を示す図、及び第３
図はとウレット法試薬の吸収曲線を示す図である．
１・・・反応テーブル ２・・・反応セル３・
・・試料注入ステーション
４・・・試薬注入ステーション
５・・・測定ステーション ６・・・光源ランプ７
・・・分光器
９・・・信号切換器
８・一光電変換素子
１２・・・演算制御回路Figure 1 is a block diagram of an apparatus showing an embodiment of the present invention, Figure 2 is a diagram showing the absorption curve of xylidi blue test straw, and Figure 3
The figure shows the absorption curve of the uret method reagent. 1... Reaction table 2... Reaction cell 3.
...Sample injection station 4...Reagent injection station 5...Measurement station 6...Light source lamp 7
... Spectrometer 9 ... Signal switch 8 - One photoelectric conversion element 12 ... Arithmetic control circuit
Claims (1)
る手段と、基準となる反応セルの試液ブランク液の等吸
収波長における吸光度を記憶する記憶手段と、試料反応
液の等吸収波長における吸光度と前記記憶手段の値との
比を求め、前記比と分析波長での試料の吸光度との積を
演算する手段とを備えてなる吸光度式自動分析装置。means for selectively measuring the absorbance at the isoabsorption wavelength and the analysis wavelength; a storage means for storing the absorbance at the isoabsorption wavelength of a sample reaction solution blank of a reference reaction cell; 1. An absorbance-type automatic analyzer, comprising means for determining a ratio with a value in a storage means and calculating the product of the ratio and the absorbance of a sample at an analysis wavelength.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1239144A JPH03102245A (en) | 1989-09-14 | 1989-09-14 | Absorbancy type automatic analyser |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1239144A JPH03102245A (en) | 1989-09-14 | 1989-09-14 | Absorbancy type automatic analyser |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03102245A true JPH03102245A (en) | 1991-04-26 |
Family
ID=17040415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1239144A Pending JPH03102245A (en) | 1989-09-14 | 1989-09-14 | Absorbancy type automatic analyser |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03102245A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10900892B1 (en) | 2020-02-17 | 2021-01-26 | SciLogica Corp. | Validation of sensor calibration |
| US11230447B2 (en) | 2015-01-23 | 2022-01-25 | Symbolic Llc | Storage and retrieval system transport vehicle |
-
1989
- 1989-09-14 JP JP1239144A patent/JPH03102245A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11230447B2 (en) | 2015-01-23 | 2022-01-25 | Symbolic Llc | Storage and retrieval system transport vehicle |
| US10900892B1 (en) | 2020-02-17 | 2021-01-26 | SciLogica Corp. | Validation of sensor calibration |
| EP3865853A1 (en) * | 2020-02-17 | 2021-08-18 | SciLogica Corp. | Validation of sensor calibration |
| WO2021165662A1 (en) * | 2020-02-17 | 2021-08-26 | SciLogica Corp. | Validation of sensor calibration |
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