JPS6298264A - Automatic analyzer - Google Patents

Abstract

PURPOSE:To improve the exactness of analysis by storing the measured values of respective wavelength components of specific analytical items with respect to each item until the analysis of samples ends and correcting the measured values of the other analytical items with the stored measured values. CONSTITUTION:For example, a reaction vessel 2 (2a-2e) handles the sample of one patient. Samples and reagents for analyzing GOT are assumed to be contained in the vessel 2a, total protein in the vessel 2b, and albumin, etc., in the vessel 2c. The vessel 2a is positioned at the intermediate between a light source 1 and a spectroscopic part 3 when the analyzing process for the entire part of the vessel 2 progresses. The measurement of absorbancy is executed and is separated into its spectral components 4. Respective wavelength components are detected 5a-5b and are stored 6 until the measurements except for the vessel 2a end. The reagent for analyzing GOT is colorless and transparent in visual sensation; therefore, the measured values of the respective wavelength components in the visible part indicate the absorbancies by the hemolysis, turbidity, jaundice, etc., of the sample. The correct absorbancy is, therefore, obtd. by subtracting the absorbancy with the GOT reaction liquid of the same wavelength components as the wavelength components of the respective analytical items.

Description

【発明の詳細な説明】 〔発明の利用分野〕 本発明は自動分析装置に関し、特に反応液に白色光を通
したのち分光し各波長成分が分散された所定の位置に複
数の受光器を置く事により同時に複数の測定を行なう多
波長光度計を使用した自動分析装置に関する。[Detailed Description of the Invention] [Field of Application of the Invention] The present invention relates to an automatic analyzer, and in particular, the present invention relates to an automatic analyzer that passes white light through a reaction solution, spectrally spectra it, and places a plurality of light receivers at predetermined positions where each wavelength component is dispersed. This invention relates to an automatic analyzer using a multi-wavelength photometer that performs multiple measurements at the same time.

〔発明の背景〕[Background of the invention]

現在多波長光度計を使用した自動分析装置における各波
長の使用方法は、各測定毎に１もしくは２の波長を選択
して使用している。試料を採取し試薬を添加し一定時間
後に測定するような分析法では試料である血清、尿など
に強い溶血や濁り、黄痕などがあった場合測定値が本来
の発色以外にそれらの影響によって増加または減少する
。この影響を除くには発色を行なわせずに試料分のみの
測定値を得るような盲検試験が必要である。盲検試験を
実施する事は処理能力が重要な省力化装置である自動分
析装置にとって処理能力を減じる事になるため、実際は
ほとんど行なわれておらず。Currently, the method of using each wavelength in an automatic analyzer using a multi-wavelength photometer is to select and use one or two wavelengths for each measurement. In analytical methods that collect a sample, add reagents, and measure after a certain period of time, if the sample (serum, urine, etc.) has strong hemolysis, turbidity, yellowing, etc., the measured value may be affected by these effects in addition to the original color. increase or decrease. In order to eliminate this influence, a blind test is required in which the measured values are obtained only for the sample without color development. In practice, blind testing is rarely done because it reduces the throughput of automatic analyzers, which are important labor-saving devices.

測定値は試料の溶血、ＮＡす、黄痘等による誤差を内在
していた。The measured values contained errors due to sample hemolysis, NA, jaundice, etc.

〔発明の目的〕[Purpose of the invention]

本発明の目的は、新たに盲検試験を実施せずに上述した
盲検試験と同等の効果を得ることができる自動分析装置
を提供することにある。An object of the present invention is to provide an automatic analyzer that can obtain the same effect as the above-mentioned blind test without conducting a new blind test.

〔発明の概要〕[Summary of the invention]

多項目自動分析装置においては１患者の試料（血清、尿
など）について複数の分析項目を同時に反応を行なわし
め、定量する事を特徴としている。この分析項目の中に
は紫外部の吸光度を測定するため可視部に吸収のない無
色透明な試薬を使用するものもある。本発明ではこの分
析項目を使用して多波長光度計の全波長成分（例えば１
２波長）の吸光度を測定し、全分析項目の測定が終わる
まで記憶しておき、他の分析項目の測定値に対し当該分
析項目の測定波長と同じ波長の吸光度を差し引いて試料
による影響を除去する。A multi-item automatic analyzer is characterized by simultaneously reacting and quantifying multiple analysis items for one patient's sample (serum, urine, etc.). Some of these analysis items use colorless and transparent reagents that have no absorption in the visible region to measure absorbance in the ultraviolet region. In the present invention, using this analysis item, all wavelength components of a multiwavelength photometer (for example, 1
2 wavelengths) and memorize it until all analysis items have been measured, and remove the influence of the sample by subtracting the absorbance at the same wavelength as the measurement wavelength of the relevant analysis item from the measured value of other analysis items. do.

〔発明の実施例〕[Embodiments of the invention]

第１図は本発明に適用した測光系の一例を示したもので
あり、１は光！ランプ、２は反応容器であり、立方体も
しくは円柱試験管状であり本容器に試料及び試薬を入れ
発色せしめたのち、そのまま測定に使用する。３は光度
計の分光部であり４は回折格子、５ａ〜５ｇは光検知器
である。また６は光検知器の電気信号を記憶するための
記憶装置、７は演算部、８は記録装置である。分析動作
は次のように行なわれる。第１図において反応容器２に
は図示されない機構によって試料、試薬が分注され化学
反応が行なわれる。１つの反応容器に所定の分注が行な
われると図中矢印の方向に回転し、２ａ、２ｂ、２ｃ、
・・・・・・の如く間欠的に次次と分注が行なわれる。Figure 1 shows an example of a photometric system applied to the present invention, where 1 indicates light! Lamp 2 is a reaction container, which is shaped like a cubic or cylindrical test tube, and is used as it is for measurement after the sample and reagent are placed in the container to develop color. 3 is a spectroscopic section of the photometer, 4 is a diffraction grating, and 5a to 5g are photodetectors. Further, 6 is a storage device for storing electrical signals from the photodetector, 7 is a calculation section, and 8 is a recording device. The analysis operation is performed as follows. In FIG. 1, a sample and a reagent are dispensed into a reaction vessel 2 by a mechanism not shown, and a chemical reaction is carried out. When a predetermined dispensing is performed in one reaction container, it rotates in the direction of the arrow in the figure, 2a, 2b, 2c,
Dispensing is performed intermittently one after another as in...

反応容器列全体はまた図示されない構造で必要により一
定温度に保温される。The entire row of reaction vessels is also kept at a constant temperature if necessary by a structure not shown.

ここで２ａ、２ｂ、２ｃ、２ｄ、２ｅが一人の患者試料
を扱っており分析項目が２ａはＧＯＴ、２ｂは総蛋白、
２ｃはアルブミン、２ｄはコレステロール、２ｅは血糖
の分析用の試料及び試薬が入っているものとする。通常
ＧＯＴの分析試薬は紫外部に吸収を持った無色透明な試
薬でありまた反応液の発色は起こらない。しかるに他の
分析項目の反応液は発色する。反応容器全体の回転矢印
方向への回転が進み、即ち分析過程が進むと、ついには
反応容器２ａは光源１と分光部３の中間に位置し吸光度
の測定が行なわれる。分光部３に入射した光は回折格子
４によって分光され光検知器５８〜５ｇにそれぞれの波
長成分として検知される。ここで１反応容器２ａのＧＯ
Ｔ分析においては全ての波長成分を必要としないがＧＯ
Ｔ分析時の全波長成分はＧＯＴ分析に使用する以外に全
て２ｂ、２ｃ、２ｄ、２ｅのそれぞれ総蛋白、アルブミ
ン、コレステロール、血糖の測定が終るまで一時的に記
憶装置６に記憶される。ＧＯＴの分析試薬は可視的には
無色透明であるから、可視部における各波長成分の測定
値は試料の溶血、濁り。Here, 2a, 2b, 2c, 2d, and 2e handle one patient sample, and the analysis items are GOT for 2a, total protein for 2b,
2c contains albumin, 2d contains cholesterol, and 2e contains samples and reagents for blood sugar analysis. Generally, GOT analytical reagents are colorless and transparent reagents that absorb in the ultraviolet region, and do not cause color development in the reaction solution. However, reaction solutions for other analysis items develop color. As the entire reaction vessel continues to rotate in the direction of the rotation arrow, that is, as the analysis process progresses, the reaction vessel 2a is finally positioned between the light source 1 and the spectroscopic section 3, and the absorbance is measured. The light incident on the spectrometer 3 is separated by the diffraction grating 4 and detected by the photodetectors 58 to 5g as respective wavelength components. Here, GO of 1 reaction vessel 2a
Although not all wavelength components are required in T analysis, GO
In addition to being used for GOT analysis, all wavelength components during T analysis are temporarily stored in the storage device 6 until the measurement of total protein, albumin, cholesterol, and blood sugar of 2b, 2c, 2d, and 2e, respectively, is completed. Since the GOT analytical reagent is visually colorless and transparent, the measured values of each wavelength component in the visible range reflect hemolysis and turbidity of the sample.

背面等による吸光度を示している事になる。従って総蛋
白、アルブミン、コレステロール、血糖の反応液即ち２
　ｂ　＋　２　ｃ　＊　２　ｄ　ｔ　２　ｅの測定時の
吸光度からそれぞれの分析項目の波長成分と同じ波長成
分のＧＯＴ反応液での吸光度を差し引けば正しい吸光度
を求める事が出来る。試料量、試薬量がＧＯＴ分析と他
の項目で異る時は各分析項目について反応液中の試料の
量の割合を求めておき補正を行なう。This shows the absorbance due to the back surface, etc. Therefore, the reaction solution of total protein, albumin, cholesterol, and blood sugar, that is, 2
Correct absorbance can be obtained by subtracting the absorbance in the GOT reaction solution of the same wavelength component as the wavelength component of each analysis item from the absorbance during measurement of b + 2 c * 2 d t 2 e. When the sample amount and reagent amount differ for GOT analysis and other items, the ratio of the sample amount in the reaction solution is determined for each analysis item and correction is performed.

〔発明の効果〕〔Effect of the invention〕

本発明によれば、臨床用多項目自動分析装置の処理能力
を減じる事がなく盲検試験を実施する事が出来、分析の
正確度を高める事が出来る。According to the present invention, a blind test can be conducted without reducing the processing capacity of a clinical multi-item automatic analyzer, and the accuracy of analysis can be improved.

【図面の簡単な説明】[Brief explanation of drawings]

第１図は本発明の一実施例の測定系の概略構成図である
。 １・・・光源ランプ、２ａ〜２ｅ・・・反応容器、３・
・・分光部、４・・・回折格子、５８〜５ｇ・・・光検
知器、６″゛＼ ・・・記憶装置。FIG. 1 is a schematic diagram of a measurement system according to an embodiment of the present invention. 1... Light source lamp, 2a to 2e... Reaction container, 3.
... Spectroscopic section, 4... Diffraction grating, 58~5g... Photodetector, 6''゛\... Storage device.

Claims (1)

【特許請求の範囲】[Claims] １、被測定試料に白色光を通過させたのち分光し、分光
された各波長成分の光量を所定の位置に複数の受光器を
置く事によつて同時に複数の測定を行なう多波長光度計
を使用した多項自動分析装置において、特定分析項目の
各波長成分の測定値を各分析項目について当該試料の全
分析が終るまで記憶しておき、他の分析項目の測定値を
記憶している測定値で補正する事を特徴とする自動分析
装置。1. A multi-wavelength photometer that allows white light to pass through the sample to be measured and then spectrally separates the light, and measures the amount of light of each separated wavelength component at the same time by placing multiple receivers at predetermined positions. In the multinomial automatic analyzer used, the measured values of each wavelength component of a specific analysis item are stored until all analyzes of the sample are completed for each analysis item, and the measured values of other analysis items are stored. An automatic analyzer characterized by correction.