JPH0257863B2 - - Google Patents
Info
- Publication number
- JPH0257863B2 JPH0257863B2 JP58057516A JP5751683A JPH0257863B2 JP H0257863 B2 JPH0257863 B2 JP H0257863B2 JP 58057516 A JP58057516 A JP 58057516A JP 5751683 A JP5751683 A JP 5751683A JP H0257863 B2 JPH0257863 B2 JP H0257863B2
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- reagent
- analysis
- sample
- automatic analyzer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims description 46
- 238000006243 chemical reaction Methods 0.000 claims description 39
- 238000004458 analytical method Methods 0.000 claims description 25
- 239000000523 sample Substances 0.000 claims description 19
- 239000000243 solution Substances 0.000 claims description 16
- 239000012490 blank solution Substances 0.000 claims description 14
- 230000003287 optical effect Effects 0.000 claims description 9
- 239000012491 analyte Substances 0.000 claims description 8
- 230000005856 abnormality Effects 0.000 claims description 7
- 239000012488 sample solution Substances 0.000 claims description 5
- 238000002835 absorbance Methods 0.000 description 20
- 239000007788 liquid Substances 0.000 description 8
- 239000013307 optical fiber Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000000149 argon plasma sintering Methods 0.000 description 3
- 230000006866 deterioration Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
- 238000011481 absorbance measurement Methods 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229960003767 alanine Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
【発明の詳細な説明】
(イ) 産業上の利用分野
この発明は、一群の試料液の各々に分析試薬を
添加し所定の条件下で反応させて生成した反応液
の光学的特性、例えばば可視光、紫外、近赤外な
どの吸光度、螢光強度、特定波長の光散乱強度な
どから被分析成分の濃度を測定する自動分析装置
であつて、分析試薬が所定の力価を有し反応が正
常に完了したことを自動的に確認しながら分析で
きるよう構成した自動分析装置に関する。[Detailed Description of the Invention] (a) Industrial Application Field This invention relates to the optical properties of a reaction solution produced by adding an analytical reagent to each of a group of sample solutions and reacting them under predetermined conditions, such as This is an automatic analyzer that measures the concentration of the analyte component based on the absorbance of visible light, ultraviolet, near-infrared, etc., fluorescence intensity, and light scattering intensity of specific wavelengths. The present invention relates to an automatic analyzer configured to perform analysis while automatically confirming that the analysis has been completed normally.
(ロ) 従来技術
従来、一群の試料液にそれぞれ分析試薬を添加
し所定の条件下で反応させて生成した反応液の光
学特性から被分析成分の濃度を測定する自動分析
装置は、その反応が完了するのに要する時間を経
過した後に各試料毎に１回宛その光学特性を測定
して分析するのが一般的である。またこれらの分
析装置には、一度に調製した相当量の分析試薬を
備蓄しその力価の劣化を防止するために分析試薬
の保冷装置を備えているものがある。そしてこの
備蓄分析試薬をすべて使いきつてから新たに調製
した分析試薬が充填される。そして自動分析装置
で多数の試料を効率よく分析するために一旦充填
した分析試薬は使用し終るまでの途中でチエツク
しない場合がある。そして試薬が劣化したままで
エンドポイント分析法（例えば血清中のグルコー
スの酵素反応による分析）を行う場合は、感度不
良によつて再現性が低下し、さらに劣化が著しく
なると真の値からずれた測定値となる。またレイ
トアツセイ法（例えばGPTの酵素活性の分析）
における装置常数（Ｋ値）を決定する場合は分析
試薬が劣化していると真の値とずれたＫ値とな
る。(B) Prior art Conventionally, automatic analyzers that measure the concentration of an analyte component from the optical properties of the reaction solution produced by adding analytical reagents to a group of sample solutions and reacting them under predetermined conditions have been used to It is common to measure and analyze the optical properties of each sample once after the time required to complete the process. Furthermore, some of these analyzers are equipped with an analytical reagent cooling device in order to store a considerable amount of analytical reagent prepared at one time and prevent the titer from deteriorating. Then, after all the stored analytical reagents are used up, newly prepared analytical reagents are filled. In order to efficiently analyze a large number of samples with an automatic analyzer, the analytical reagents once filled may not be checked until they are finished being used. When performing an endpoint analysis method (for example, analysis of glucose in serum using an enzymatic reaction) while the reagent has deteriorated, reproducibility decreases due to poor sensitivity, and if the reagent deteriorates significantly, it may deviate from the true value. This is the measured value. Also late assay method (e.g. analysis of GPT enzymatic activity)
When determining the device constant (K value) for , if the analytical reagent has deteriorated, the K value will deviate from the true value.
(ハ) 発明の目的
この発明は記のような問題点を解消するために
なされたものであつて、分析試薬が所定の力価を
有し反応が正常に完了したことを一試料の分析毎
に自動的に確認しながら分析できる自動分析装置
を提供することを目的とするものである。(C) Purpose of the Invention This invention was made to solve the problems mentioned above, and it is an object of the present invention to confirm that the analytical reagent has a predetermined titer and that the reaction has completed normally after every analysis of one sample. The purpose of this invention is to provide an automatic analyzer that can perform analysis while automatically checking the results.
(ニ) 発明の構成
この発明は、被分析成分を含有する多数の試料
液に所定の分析試薬を添加し所定の条件下で反応
させて生成した反応液の光学的特性から被分析成
分の濃度を連続的もしくは間欠的に定量する自動
分析装置であつて、
上記反応液および試薬ブランク液の光学的特性
を、分析試薬を添加してから任意にきめた時間
（t1，t2，……，to-1，to）経過後に複数回測定す
る手段と、t1，t2，……，to-1，toにおける該反応
液の測定値At1，At2，……Ato-1，Atoおよび試
薬ブランク液の測定値AR1，AR2，……，
ARo-1，ARoについて、式：
｜At1−AR1｜、
｜At1−AR1／Ato−ARo｜、
｜At2−AR2／Ato−ARo｜、
〓
〓
および｜Ato-1−ARo-1／Ato−ARo｜
で表される数値を算出し、各数値が所定のしきい
値以上であるか否かを判定して各数値がいずれも
しきい値以上であればAto値から被分析成分の濃
度に換算して表示し、一方各算出値のいずれかが
しきい値以下であれば分析異常であることを表示
もしくは通報する手段を具備し、分析試薬が所定
の力価を有し反応が正常に完了したことを一試料
の分析毎に自動的に確認しながら分析できるよう
構成したことを特徴とする自動分析装置を提供す
るものである。(d) Structure of the Invention This invention is a method for determining the concentration of an analyte component from the optical characteristics of a reaction solution produced by adding a predetermined analytical reagent to a large number of sample liquids containing the analyte component and reacting them under predetermined conditions. An automatic analyzer that continuously or intermittently determines the optical properties of the reaction solution and reagent blank solution at an arbitrarily determined time (t 1 , t 2 ,...) after adding the analytical reagent. , t o-1 , t o ), and the measured values of the reaction solution at t 1 , t 2 , ..., t o-1 , t o ) At 1 , At 2 , ... At o-1 , At o and measured values of reagent blank solution AR 1 , AR 2 , ...,
For AR o-1 and AR o , the formula: |At 1 −AR 1 |, |At 1 −AR 1 /At o −AR o |, |At 2 −AR 2 /At o −AR o |, 〓 〓 and ｜At o-1 −AR o-1 /At o −AR o If it is above the threshold value, the concentration of the component to be analyzed is converted from the At o value and displayed, while if any of the calculated values is below the threshold value, it is equipped with a means to display or notify that there is an analysis abnormality. The present invention provides an automatic analyzer characterized in that it is configured so that analysis can be performed while automatically confirming that the analytical reagent has a predetermined titer and that the reaction has completed normally for each sample analysis. .
(ホ) 実施例
以下、図に示す実施例に基づいて、この発明を
説明する。ただしこれによりこの発明が限定され
るものではない。(e) Embodiments The present invention will be described below based on embodiments shown in the drawings. However, this invention is not limited to this.
第１図は、反応液の吸光度を測定して被分析成
分の濃度を測定する。この発明の自動分析装置の
一実施例の構成説明図である。 In FIG. 1, the concentration of the analyte component is determined by measuring the absorbance of the reaction solution. FIG. 1 is a configuration explanatory diagram of an embodiment of an automatic analyzer of the present invention.
まず一回の分析に用いる試料の液量と同量の純
水もしくは生理的食塩水１がそのピペツタ２で採
取され反応兼測定容器ライン３の容器４に採取さ
れ順次矢印ａの方向に駆動されて移動する。次い
でこの容器にひとつの試料の分析に用いる所定量
の分析試薬５をそのピペツタ６で採取して注入し
試薬ブランク液とする。一方別の容器７に、試料
架設テーブル８上に並べられた一群の同種試料の
１容器から試料採取ピペツタ９が所定量の第１号
試料液を分取し、順次矢印ａの方向に駆動されて
移動する。次いでこの容器７に分析試薬ピペツタ
６によつて採取された所定量の分析試薬５を注入
し反応を開始させ、生成した第１号試料反応液を
順次矢印ａ方向に駆動して移動させる。次いで以
後の反応兼測定容器に別の第２……ｍ号試料液の
所定量を、順次上記と同様にして採取し分析試薬
５を添加して反応を開始させ、第２〜ｍ号試料の
反応液を作製して順次矢印ａの方向に移動させ
る。 First, the same amount of pure water or physiological saline 1 as the sample liquid volume used for one analysis is collected with the pipette 2, placed in the container 4 of the reaction/measuring container line 3, and sequentially driven in the direction of arrow a. and move. Next, a predetermined amount of analytical reagent 5 used for the analysis of one sample is collected and injected into this container using the pipette 6 to serve as a reagent blank solution. On the other hand, the sample collection pipette 9 dispenses a predetermined amount of the first sample liquid from one container of a group of similar samples arranged on the sample mounting table 8 into another container 7, and is sequentially driven in the direction of the arrow a. and move. Next, a predetermined amount of the analytical reagent 5 collected by the analytical reagent pipette 6 is injected into the container 7 to start a reaction, and the generated No. 1 sample reaction liquid is sequentially driven and moved in the direction of the arrow a. Next, a predetermined amount of another No. 2...m sample solution is sequentially collected in the subsequent reaction/measuring container in the same manner as above, and the analysis reagent 5 is added to start the reaction. A reaction solution is prepared and sequentially moved in the direction of arrow a.
順次移動されて、試薬ブランク液作製後の所定
時間t1経過した試薬ブランク液の容器がＡの位置
に達した時、光源ランプ１０の光がオプテイカル
フアイバー１１を通じて該試薬ブランク液を透過
してからフイルター１２を透過し所定の光のみを
透過し検出器１３に入る。さらに上記試薬ブラン
ク液が試薬ブランク液作製時から所定の時間t2経
過してからＢ位置に達したとき上記と同様に光源
ランプ１０→オプテイカルフアイバー１４−Ｂ位
置の試薬ブランク液→フイルター１５→検出器１
６の経路で透過光が検出器１６に入る。さらにこ
の試薬ブランク液容器が試薬ブランク液作製から
所定の時間t3経過してＣの位置に達した時に上記
と同様に光源ランプ１０→オプテイカルフアイバ
ー１７→試薬ブランク液→フイルター１８−検出
器１９の経路で透過光が検出器１９に入る。 When the container of the reagent blank liquid is sequentially moved and reaches the position A after a predetermined time t1 has elapsed after the preparation of the reagent blank liquid, the light from the light source lamp 10 passes through the reagent blank liquid through the optical fiber 11. The light passes through the filter 12, and only a predetermined amount of light is transmitted, and enters the detector 13. Furthermore, when the reagent blank solution reaches position B after a predetermined time t2 has elapsed since the reagent blank solution was prepared, the light source lamp 10 → the reagent blank solution at the optical fiber 14-B position → the filter 15 → Detector 1
The transmitted light enters the detector 16 through the path 6. Furthermore, when this reagent blank liquid container reaches the position C after a predetermined time t3 has elapsed since the preparation of the reagent blank liquid, the light source lamp 10→optical fiber 17→reagent blank solution→filter 18-detector 19 The transmitted light enters the detector 19 along the path.
各検出器１３，１６，１９からの透過光の信号
はマルチプレクサ２０に入り、標準の特定の波長
が選択され次いで吸光度変換器２１を経て吸光度
メモリー部２３に入つて、t1，t2およびt3の時間
経過後における試薬ブランク液の各吸光度A1R1，
A1R2およびA1R3が記憶される。 The transmitted light signal from each detector 13, 16, 19 enters a multiplexer 20, selects a standard specific wavelength, and then passes through an absorbance converter 21 to an absorbance memory section 23 at t 1 , t 2 and t Each absorbance of the reagent blank solution A 1 R 1 after 3 hours elapsed,
A 1 R 2 and A 1 R 3 are stored.
一方第１号試料の反応液が順次移動されて反応
開始後の所定時間t1経過してＡの位置に達した
時、前記と同様に光源ランプ１０→オプテイカル
フアイバー１１→Ａ位置の第１号試料反応液→フ
イルター１２→検出器１３の経路で透過光が検出
器１３に入る。次いで反応開始から所定時間t2の
時間経過してＢの位置に達した時、光源ランプ１
０→オプテイカルフアイバー１４→Ｂ位置の第１
号試料反応後→フイルター１５→検出器１６の経
路で透過光が検出器１６に入る。さらに反応開始
後の所定時間t3経過してＣ位置に達したとき光源
ランプ１０→オプテイカルフアイバー１７→Ｃ位
置の第１号試料反応液→フイルター１８→検出器
１９の経路で透過光が検出器１９に入る。そして
各検出器１３，１６，１９からの透過光の信号は
マルチプレクサ２０に入り、標準の特定の波長が
選択され、次いで吸光度変換器２１を経て吸光度
の信号に変換され吸光度メモリー部２３に入つて
第１号試料反応液の反応開始後t1，t2およびt3の
時間間経過後の各吸光度A1t1，A1t2およびA1t3が
記憶される。 On the other hand, when the reaction solution of the first sample is sequentially moved and reaches the position A after a predetermined time t1 has elapsed after the start of the reaction, the light source lamp 10 → optical fiber 11 → the first The transmitted light enters the detector 13 through the path of No. sample reaction solution → filter 12 → detector 13. Next, when the predetermined time t 2 has passed from the start of the reaction and the position B is reached, the light source lamp 1 is turned on.
0→Optical fiber 14→1st position B
After the reaction of the No. sample, the transmitted light enters the detector 16 through the following path: filter 15 → detector 16. Furthermore, when the predetermined time t 3 after the start of the reaction has passed and the position C is reached, transmitted light is detected along the path of light source lamp 10 → optical fiber 17 → No. 1 sample reaction liquid at position C → filter 18 → detector 19. Enter vessel 19. The transmitted light signals from each detector 13, 16, 19 enter the multiplexer 20, select a standard specific wavelength, and then pass through the absorbance converter 21, convert it into an absorbance signal, and enter the absorbance memory section 23. The respective absorbances A 1 t 1 , A 1 t 2 and A 1 t 3 after the elapse of time t 1 , t 2 and t 3 after the start of the reaction of the first sample reaction solution are stored.
次いで吸光度メモリー部２３に記憶された上記
の各吸光度は吸光度判定部２４に入り、下記式
（）〜（）の左辺で表される数値が算出され
る。 Next, each of the above absorbances stored in the absorbance memory section 23 enters the absorbance determining section 24, where the numerical values represented by the left sides of the following equations () to () are calculated.
｜A1t1−A1R1｜＞V1 （）
｜A1t1−A1R1／A1t3−A1R3｜＞V2 （）
｜A1t2−A1R2／A1t3−A1R3｜＞V3 （）
そしてまず（）式左辺の算出数値が予め記憶
させた所定のしきい値V1以上であるか否かが判
定されV1以下であれば表示部２６に異常の信号
が伝えられ表示部２６が異常を表示する。また
V1以上であれば続いて（）式左辺の算出数値
が予め記憶させた所定のしきい値V2以上である
か否か判定されV2以下であれば上記と同様にし
て異常が表示される。またV2以上であれば続い
て（）式の左辺の算出数値が予め記憶されたし
きい値V3以上であるか否かが判定され、V3以下
であれば上記と同様に異常が表示される。なお異
常が表示された時に分析が自動的に停止されおよ
び／または測定値が印字される。さらにV3以上
であれば吸光度A1t3の信号が濃度変換器２５を経
て表示部２６に送られ被分析成分濃度の分析結果
が表示および／または印字される。 ｜A 1 t 1 −A 1 R 1 ｜＞V 1 () ｜A 1 t 1 −A 1 R 1 /A 1 t 3 −A 1 R 3 ｜＞V 2 () ｜A 1 t 2 −A 1 R 2 /A 1 t 3 −A 1 R 3 |>V 3 () First, it is determined whether the calculated value on the left side of equation ( ) is greater than or equal to a predetermined threshold value V 1 stored in advance. If it is below, an abnormality signal is transmitted to the display unit 26, and the display unit 26 displays the abnormality. Also
If V 1 or more, then it is determined whether the calculated value on the left side of the equation () is greater than or equal to a predetermined threshold V 2 stored in advance, and if V 2 or less, an abnormality is displayed in the same manner as above. Ru. Also, if V 2 or more, it is then determined whether the calculated value on the left side of the equation () is greater than or equal to a pre-stored threshold value V 3 , and if V 3 or less, an abnormality is displayed in the same way as above. be done. Note that when an abnormality is displayed, the analysis is automatically stopped and/or the measured value is printed. Furthermore, if it is V3 or more, a signal of absorbance A1t3 is sent to the display unit 26 via the concentration converter 25, and the analysis result of the concentration of the analyte is displayed and/or printed.
この第１号試料と同様の手順によつて第２〜ｍ
号の試料も処理され、異常が表示されると分析が
自動的に停止するかおよび／または測定値を印字
する。 By the same procedure as this No. 1 sample, No. 2 to m
No. samples are also processed, and if an anomaly is indicated, the analysis will automatically stop and/or the measured value will be printed.
なお上記の一連の操作は制御部２７で行われ
る。 Note that the above series of operations are performed by the control section 27.
試薬ブランク液の吸光度測定は上記のように一
群の試料液を連続的に測定し始める最初に一回測
定するだけでよい。 The absorbance measurement of the reagent blank solution only needs to be performed once at the beginning of the continuous measurement of a group of sample solutions as described above.
t1としては分析試薬の力価が正常であれば、分
析に利用する反応が40〜60％程度完了している時
間が選ばれる。t2としては反応率が70〜90％完了
している時間が選ばれ、t3としては反応が充分完
了している時間が選択される。 As t1 , if the titer of the analytical reagent is normal, the time is selected at which approximately 40 to 60% of the reaction used for analysis is completed. As t 2 , a time is selected when the reaction rate is 70-90% complete, and as t 3 , a time is selected when the reaction is fully completed.
また各しきい値V1，V2およびV3は上記の時間
t1〜t3に基づいて反応が正常に進行しているかど
うかをチエツクできるように適宜選択される。 In addition, each threshold value V 1 , V 2 and V 3 is determined by the above time.
It is appropriately selected so that it can be checked whether the reaction is progressing normally based on t1 to t3 .
第２図は試薬ブランク液調製または反応開始時
間t0からt1〜t3経過後の試薬ブランク液と第１号
試料反応液の吸光度を示すグラフであり、曲線
x1，x2は分析試薬の力価が劣化した場合のグラフ
である。 FIG. 2 is a graph showing the absorbance of the reagent blank solution and the No. 1 sample reaction solution after t 1 to t 3 have elapsed from the reagent blank solution preparation or reaction start time t 0 , and the curve
x 1 and x 2 are graphs when the titer of the analytical reagent deteriorates.
なお試薬ブランク液や試料反応液の時間経過後
の測定回数はその分析に利用する反応の種類によ
つて左右されるが一般に３〜４回で充分である。 The number of times the reagent blank solution or sample reaction solution is measured over time depends on the type of reaction used for the analysis, but generally 3 to 4 times is sufficient.
上記の吸光度によつて分析する具体例として
は、血清または血漿中のグルコースの分析が挙げ
られ、下記の反応式に基づいて生成したキノン色
素を波長510nmの光の吸光度で測定される。 A specific example of analysis using the above absorbance is the analysis of glucose in serum or plasma, in which a quinone dye produced based on the following reaction formula is measured using the absorbance of light at a wavelength of 510 nm.
グルコース＋2H2Oグルコースオキシダーゼ
―――――――――――――――→
グルコン酸＋H2O2
2H2O2＋フエノール＋４−アミノアンチピリンパーオ
キシダーゼ
―――――――――――→
キノン色素＋4H2O
他の具体例としては、GPTの酵素活性を下記
反応に基づいて分析する場合に、反応式（Ｂ）に
基いてNADHの340nm近傍の波長光の吸光度で
装置定数（Ｋ値）を測定する場合が挙げられる。 Glucose + 2H 2 O glucose oxidase――――――――――――――→ Gluconic acid + H 2 O 2 2H 2 O 2 + Phenol + 4-aminoantipyrine peroxidase―――――――――― -→ Quinone dye + 4H 2 O As another specific example, when analyzing the enzymatic activity of GPT based on the following reaction, the device constant ( K value) is measured.
Ｌ−アラニン＋α−ケトグルタル酸GPT
――――→
グルタミン酸＋ピルビン酸 (A)
ピルビン酸＋NADHLDH
――――→
乳酸＋NAD (B)
この場合は分析試薬の劣化の、測定値に対する
影響が大きいのでこの自動分析装置によつて測定
すれば特に有利である。 L-alanine + α-ketoglutarate GPT ――――→ Glutamic acid + Pyruvate (A) Pyruvate + NADHLDH ――――→ Lactic acid + NAD (B) In this case, the deterioration of the analytical reagent has a large effect on the measured value, so this method is recommended. It is particularly advantageous if the determination is made with an automatic analyzer.
上記実施例は吸光度測定によるものであるが、
この外の光学特性として反応液の螢光強度や光散
乱強度を利用する場合は第１図における点線で囲
まれた光学特性測定ブロツク２８が螢光強度や光
散乱強度測定用の系に変換するとともにこれに対
応して他の系も適宜修正される。 The above example is based on absorbance measurement, but
When using the fluorescence intensity or light scattering intensity of the reaction solution as other optical properties, the optical property measurement block 28 surrounded by the dotted line in Fig. 1 is converted into a system for measuring the fluorescence intensity or light scattering intensity. At the same time, other systems are also modified accordingly.
(ヘ) 発明の効果
この発明の自動分析装置によれば、分析試薬の
劣化を自動的にチエツクしかつ反応が正常に完了
したことを確認しながら多数の試料を連続的に分
析することができる。従つて試薬の劣化による再
現性不良の測定値とか真の値からずれた測定値を
与えるような分析を避けることができる。(f) Effects of the invention According to the automatic analyzer of this invention, it is possible to continuously analyze a large number of samples while automatically checking for deterioration of analytical reagents and confirming that the reaction has completed normally. . Therefore, it is possible to avoid an analysis that gives a measured value with poor reproducibility or a measured value that deviates from the true value due to deterioration of the reagent.
第１図はこの発明の自動分析装置の一実施例の
構成説明図、第２図は第１図の装置によつてエン
ドポイント法で測定した場合の吸光度と時間との
関係を示すグラフである。
１……純水もしくは生理的食塩水、２，６，９
……ピペツタ、５……分析試薬、３……反応兼測
定容器ライン、４，７……反応兼測定容器、８…
…試料架設テーブル、１０……光源ランプ、１
１，１４，１７……オプテイカルフアイバー、１
２，１５，１８……フイルター、１３，１６，１
９……検出器、２０……マルチプレクサ、２１…
…吸光度変換器、２３……吸光度メモリー部、２
４……吸光度判定部、２５……濃度変換器、２６
……表示部、および２７……制御部。
Fig. 1 is an explanatory diagram of the configuration of one embodiment of the automatic analyzer of the present invention, and Fig. 2 is a graph showing the relationship between absorbance and time when measured by the end point method using the apparatus shown in Fig. 1. . 1...Pure water or physiological saline, 2, 6, 9
...Pippet, 5...Analysis reagent, 3...Reaction and measurement container line, 4, 7...Reaction and measurement container, 8...
...Sample installation table, 10...Light source lamp, 1
1, 14, 17...Optical fiber, 1
2, 15, 18... Filter, 13, 16, 1
9...detector, 20...multiplexer, 21...
...Absorbance converter, 23...Absorbance memory section, 2
4... Absorbance determination section, 25... Concentration converter, 26
. . . display section, and 27 . . . control section.
Claims (1)
分析試薬を添加し所定の条件下で反応させて生成
した反応液の光学的特性から被分析成分の濃度を
連続的もしくは間欠的に定量する自動分析装置で
あつて、 上記反応液および試薬ブランク液の光学的特性
を、分析試薬を添加してから任意にきめた時間
（t1，t2，……，to-1，to）経過後に複数回測定す
る手段と、t1，t2，……，to-1，toにおける該反応
液の測定値At1，At2，……Ato-1，Atoおよび試
薬ブランク液の測定値AR1，AR2，……，
ARo-1，ARoについて、式： ｜At1−AR1｜、 ｜At1−AR1／Ato−ARo｜、 ｜At2−AR2／Ato−ARo｜、 〓 〓 および｜Ato-1−ARo-1／Ato−ARo｜ で表される数値を算出し、各数値が所定のしきい
値以上であるか否かを判定して各数値がいずれも
しきい値以上であればAto値から被分析成分の濃
度に換算して表示し、一方各算出値のいずれかが
しきい値以下であれば分析異常であることを表示
もしくは通報する手段を具備し、分析試薬が所定
の力価を有し反応が正常に完了したことを一試料
の分析毎に自動的に確認しながら分析できるよう
構成したことを特徴とする自動分析装置。[Claims] 1. Continuous determination of the concentration of the analyte component from the optical properties of the reaction solution produced by adding a predetermined analytical reagent to a large number of sample solutions containing the analyte component and reacting them under predetermined conditions. Alternatively, it is an automatic analyzer that performs intermittently quantitative determination, and the optical properties of the reaction solution and reagent blank solution are determined at an arbitrarily determined time (t 1 , t 2 ,..., t o ) after adding the analytical reagent. -1 , t o ), and the measured values of the reaction solution at t 1 , t 2 , ..., t o-1 , t o , At 1 , At 2 , ... At o-1 , At o and the measured values of the reagent blank solution AR 1 , AR 2 , ...,
For AR o-1 and AR o , the formula: |At 1 −AR 1 |, |At 1 −AR 1 /At o −AR o |, |At 2 −AR 2 /At o −AR o |, 〓 〓 and ｜At o-1 −AR o-1 /At o −AR o If any of the calculated values is above the threshold value, the concentration of the analyzed component is converted and displayed, and if any of the calculated values is below the threshold value, it is equipped with a means to display or notify that there is an analysis abnormality. . An automatic analyzer characterized in that the automatic analyzer is configured to be able to perform analysis while automatically confirming that the analytical reagent has a predetermined titer and that the reaction has completed normally for each analysis of one sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5751683A JPS59182347A (en) | 1983-03-31 | 1983-03-31 | Automatic analytical instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5751683A JPS59182347A (en) | 1983-03-31 | 1983-03-31 | Automatic analytical instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59182347A JPS59182347A (en) | 1984-10-17 |
| JPH0257863B2 true JPH0257863B2 (en) | 1990-12-06 |
Family
ID=13057898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5751683A Granted JPS59182347A (en) | 1983-03-31 | 1983-03-31 | Automatic analytical instrument |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59182347A (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2510152B2 (en) * | 1985-11-19 | 1996-06-26 | オリンパス光学工業株式会社 | Automatic analyzer |
| JPS63502931A (en) * | 1986-03-26 | 1988-10-27 | ベックマン インスツルメンツ インコーポレーテッド | Automated multi-purpose analytical chemistry processing facility and laboratory work equipment |
| JP2661017B2 (en) * | 1986-07-28 | 1997-10-08 | 株式会社島津製作所 | Automatic analyzer |
| JPS63158459A (en) * | 1986-09-05 | 1988-07-01 | ライフトレイク | Method of adjusting precision of sensitive assay |
| JP2656564B2 (en) * | 1988-08-26 | 1997-09-24 | 株式会社日立製作所 | Immunoassay method |
| JP4966879B2 (en) * | 2008-01-24 | 2012-07-04 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5686355A (en) * | 1979-12-14 | 1981-07-14 | Jeol Ltd | Automatic chemical analysis |
-
1983
- 1983-03-31 JP JP5751683A patent/JPS59182347A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5686355A (en) * | 1979-12-14 | 1981-07-14 | Jeol Ltd | Automatic chemical analysis |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS59182347A (en) | 1984-10-17 |
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