HK1217032A1 - 用於修飾核酸的試劑盒 - Google Patents

用於修飾核酸的試劑盒

Info

Publication number
HK1217032A1
HK1217032A1 HK16105045.6A HK16105045A HK1217032A1 HK 1217032 A1 HK1217032 A1 HK 1217032A1 HK 16105045 A HK16105045 A HK 16105045A HK 1217032 A1 HK1217032 A1 HK 1217032A1
Authority
HK
Hong Kong
Prior art keywords
kits
nucleic acids
modifying nucleic
modifying
acids
Prior art date
Application number
HK16105045.6A
Other languages
English (en)
Inventor
杰羅馬‧箭之撒
加力‧大鹿
海纓‧李‧格魯內瓦德
猊克拉士‧鎧如酋
Original Assignee
Epicentre Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42119714&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HK1217032(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Epicentre Tech Corp filed Critical Epicentre Tech Corp
Publication of HK1217032A1 publication Critical patent/HK1217032A1/zh

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK16105045.6A 2008-10-24 2016-05-03 用於修飾核酸的試劑盒 HK1217032A1 (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10832908P 2008-10-24 2008-10-24
US10832108P 2008-10-24 2008-10-24
US10832608P 2008-10-24 2008-10-24
US15286809P 2009-02-16 2009-02-16
US15543109P 2009-02-25 2009-02-25
US18453009P 2009-06-05 2009-06-05

Publications (1)

Publication Number Publication Date
HK1217032A1 true HK1217032A1 (zh) 2016-12-16

Family

ID=42119714

Family Applications (6)

Application Number Title Priority Date Filing Date
HK12103051.6A HK1163107A1 (en) 2008-10-24 2012-03-28 Transposon end compositions and methods for modifying nucleic acids
HK12110399.2A HK1169659A1 (en) 2008-10-24 2012-10-18 Transposon end compositions and methods for modifying nucleic acids
HK14110396.3A HK1197498A1 (zh) 2008-10-24 2014-10-17 轉座子末端組成和改變核酸的方法
HK16105045.6A HK1217032A1 (zh) 2008-10-24 2016-05-03 用於修飾核酸的試劑盒
HK16107659.9A HK1219806A1 (zh) 2008-10-24 2016-06-30 轉座子末端組成和改變核酸的方法
HK18108684.4A HK1249765B (zh) 2008-10-24 2018-07-05 轉座端組合及改動核酸的方法

Family Applications Before (3)

Application Number Title Priority Date Filing Date
HK12103051.6A HK1163107A1 (en) 2008-10-24 2012-03-28 Transposon end compositions and methods for modifying nucleic acids
HK12110399.2A HK1169659A1 (en) 2008-10-24 2012-10-18 Transposon end compositions and methods for modifying nucleic acids
HK14110396.3A HK1197498A1 (zh) 2008-10-24 2014-10-17 轉座子末端組成和改變核酸的方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
HK16107659.9A HK1219806A1 (zh) 2008-10-24 2016-06-30 轉座子末端組成和改變核酸的方法
HK18108684.4A HK1249765B (zh) 2008-10-24 2018-07-05 轉座端組合及改動核酸的方法

Country Status (13)

Country Link
EP (6) EP2787565B1 (zh)
JP (1) JP5794572B2 (zh)
CN (2) CN105018548B (zh)
CA (1) CA2750054C (zh)
DK (4) DK2376517T3 (zh)
ES (5) ES2403756T3 (zh)
HK (6) HK1163107A1 (zh)
HU (1) HUE027961T2 (zh)
LT (1) LT2963709T (zh)
PL (1) PL2963709T3 (zh)
PT (2) PT2787565E (zh)
SI (1) SI2963709T1 (zh)
WO (1) WO2010048605A1 (zh)

Families Citing this family (160)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009091719A1 (en) 2008-01-14 2009-07-23 Applera Corporation Compositions, methods, and kits for detecting ribonucleic acid
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
ES2565563T3 (es) * 2010-02-25 2016-04-05 Advanced Liquid Logic, Inc. Método para preparar bibliotecas de ácidos nucleicos
US20110224106A1 (en) * 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production Of Single-Stranded Circular Nucleic Acid
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
WO2012061832A1 (en) * 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
CA2826131C (en) * 2011-02-02 2019-11-05 Jay Ashok Shendure Massively parallel continguity mapping
US9005935B2 (en) * 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
EP3517611A3 (en) * 2011-06-15 2019-09-04 Gen9, Inc. Methods for preparative in vitro cloning
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
CA2844056A1 (en) * 2011-08-04 2013-02-07 Sage Science, Inc. Systems and methods for processing fluids
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
EP2783001B1 (en) * 2011-11-22 2018-01-03 Active Motif Multiplex isolation of protein-associated nucleic acids
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
NO2694769T3 (zh) * 2012-03-06 2018-03-03
CN102703426A (zh) * 2012-05-21 2012-10-03 吴江汇杰生物科技有限公司 构建核酸库的方法、试剂及试剂盒
WO2013181265A1 (en) * 2012-05-29 2013-12-05 The Johns Hopkins University Eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries
CN102703432A (zh) * 2012-07-11 2012-10-03 烟台博诺生物科技有限公司 构建核酸库的方法、试剂及试剂盒
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
JP6141989B2 (ja) 2012-10-12 2017-06-07 セイジ サイエンス,インコーポレイティド 側方溶出型分子分画装置
US9868946B2 (en) 2012-11-05 2018-01-16 Icahn School Of Medicine At Mount Sinai Method of isolating pure mitochondrial DNA
JP6165875B2 (ja) * 2012-12-06 2017-07-19 上海 インスティテューツ フォー バイオロジカル サイエンシーズ、チャイニーズ アカデミー オブ サイエンシーズShanghai Institutes For Biological Sciences, Chinese Academy Of Sciences 一連の糖転移酵素およびその応用
JP6410726B2 (ja) 2012-12-10 2018-10-24 レゾリューション バイオサイエンス, インコーポレイテッド 標的化ゲノム解析のための方法
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) * 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
ES2724824T3 (es) 2013-03-13 2019-09-16 Illumina Inc Métodos para la secuenciación de ácidos nucleicos
US9328382B2 (en) * 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014171898A2 (en) * 2013-04-17 2014-10-23 Agency For Science, Technology And Research Method for generating extended sequence reads
EP2999784B2 (en) * 2013-05-22 2022-11-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
CN105339503B (zh) 2013-05-23 2020-04-10 斯坦福大学托管董事会 用于个人表观基因组学的至天然染色质的转座
US10655167B2 (en) 2013-07-26 2020-05-19 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9217167B2 (en) * 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
JP6626830B2 (ja) 2013-11-07 2019-12-25 アジレント・テクノロジーズ・インクAgilent Technologies, Inc. Dna操作のための複数のトランスポザーゼアダプター
US10597715B2 (en) * 2013-12-05 2020-03-24 Centrillion Technology Holdings Methods for sequencing nucleic acids
CN103710342A (zh) * 2013-12-18 2014-04-09 浙江农林大学 人工诱导的mle转座子试剂盒及其应用
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
EP3094741A1 (en) * 2014-01-14 2016-11-23 Qiagen GmbH Generation of tagged dna fragments
WO2015113725A1 (en) * 2014-02-03 2015-08-06 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
US11136576B2 (en) 2014-02-03 2021-10-05 Thermo Fisher Scientific Baltics Uab Method for controlled DNA fragmentation
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20170114333A1 (en) * 2014-04-11 2017-04-27 The Johns Hopkins University Improvements to eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries
WO2015160895A2 (en) * 2014-04-15 2015-10-22 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased dna input tolerance
US20170044525A1 (en) 2014-04-29 2017-02-16 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
CN107075509B (zh) 2014-05-23 2021-03-09 数字基因公司 通过数字化转座子的单倍体组测定
CA2952058A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
SG11201610910QA (en) 2014-06-30 2017-01-27 Illumina Inc Methods and compositions using one-sided transposition
CN106715713B (zh) * 2014-09-12 2020-11-03 深圳华大智造科技有限公司 试剂盒及其在核酸测序中的用途
WO2016037358A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
CN105525357B (zh) * 2014-09-30 2018-08-21 深圳华大基因股份有限公司 一种测序文库的构建方法及试剂盒和应用
GB201418159D0 (en) * 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN107075560B (zh) * 2014-10-14 2021-01-26 深圳华大智造科技股份有限公司 一种核酸的转座酶打断一站式处理方法及试剂
JP2017534297A (ja) * 2014-10-14 2017-11-24 ビージーアイ シェンヂェン カンパニー リミテッドBgi Shenzhen Co., Limited トランスポゼースによる核酸の切断及びリンカー付加の方法及び試薬
US10093955B2 (en) 2014-10-14 2018-10-09 Covaris, Inc. Acoustic energy mediation of genetic fragmentation
JP6612340B2 (ja) 2014-10-15 2019-11-27 セージ サイエンス, インコーポレイテッド 核酸の自動化された加工処理および電気泳動による試料調製のための装置、方法およびシステム
ES2745814T3 (es) * 2014-10-17 2020-03-03 Illumina Cambridge Ltd Transposición conservadora de contigüidad
JP6808617B2 (ja) * 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
ES2947757T3 (es) 2014-11-05 2023-08-18 Illumina Inc Composiciones de transposasas para reducir el sesgo de inserción
DK3218513T3 (en) 2014-11-11 2019-02-04 Illumina Inc Polynucleotide amplification using CRISPR-CAS systems
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和***以及用其制备的文库
CN105985945B (zh) * 2015-01-30 2019-04-23 深圳华大智造科技有限公司 mRNA片段化方法及基于其构建测序文库的方法
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016169431A1 (zh) * 2015-04-20 2016-10-27 深圳华大基因研究院 一种长片段dna文库构建方法
EP3303621A1 (en) 2015-05-28 2018-04-11 Illumina Cambridge Limited Surface-based tagmentation
US10640809B2 (en) 2015-05-29 2020-05-05 Epicentre Technologies Corporation Methods of analyzing nucleic acids
US10280449B2 (en) 2015-06-29 2019-05-07 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
MX2018000729A (es) 2015-07-17 2018-09-06 Harvard College Métodos para amplificar las secuencias de ácido nucleico.
CA2994958C (en) 2015-08-07 2024-02-13 Massachusetts Institute Of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
CA2997035A1 (en) 2015-08-28 2017-03-09 Illumina, Inc. Nucleic acid sequence analysis from single cells
US11542495B2 (en) 2015-11-20 2023-01-03 Sage Science, Inc. Preparative electrophoretic method for targeted purification of genomic DNA fragments
ES2926495T3 (es) 2015-12-04 2022-10-26 10X Genomics Inc Métodos y composiciones para el análisis de ácidos nucleicos
CN105524920B (zh) * 2016-01-21 2018-10-02 深圳华大基因研究院 用于Ion Proton测序平台的TN5建库的引物组、试剂盒和建库方法
EP4269611A3 (en) 2016-05-11 2024-01-17 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
US10240196B2 (en) 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
JP6943376B2 (ja) * 2016-08-30 2021-09-29 国立大学法人九州大学 Dna結合タンパク質の結合領域の近傍に所望のdna断片を挿入する方法
CN110139932B (zh) * 2016-12-19 2024-05-17 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20190352609A1 (en) * 2017-01-05 2019-11-21 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Method for preparing reticulocyte simulating particles and platelet simulating particles, and reference control
CN106801044B (zh) * 2017-01-09 2021-03-23 北京全式金生物技术有限公司 一种环状转座子复合物及其应用
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和***
IL263118B2 (en) 2017-02-21 2023-12-01 Illumina Inc Tegumentation using fixed transpososomes with linkers
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
WO2018187779A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
WO2018217625A1 (en) * 2017-05-23 2018-11-29 Bio-Rad Laboratories, Inc. Molecular barcoding
JP2020522243A (ja) * 2017-05-23 2020-07-30 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸のマルチプレックス末端タギング増幅
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2019023214A1 (en) * 2017-07-25 2019-01-31 Massachusetts Institute Of Technology ATAC IN SITU SEQUENCING
CN107586835B (zh) * 2017-10-19 2020-11-03 东南大学 一种基于单链接头的下一代测序文库的构建方法及其应用
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11873374B2 (en) 2018-02-06 2024-01-16 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
US11807903B2 (en) * 2018-02-08 2023-11-07 Universal Sequencing Technology Corporation Methods and compositions for tracking nucleic acid fragment origin for nucleic acid sequencing
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
CN110219054B (zh) * 2018-03-04 2020-10-02 清华大学 一种核酸测序文库及其构建方法
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US20210071240A1 (en) 2018-04-19 2021-03-11 Massachusetts Institute Of Technology Single-stranded break detection in double-stranded dna
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN113249796A (zh) * 2018-06-20 2021-08-13 深圳海普洛斯医学检验实验室 对dna片段进行标记的单分子标签
DK3821011T3 (da) * 2018-07-11 2023-07-24 Cygnus Biosciences Beijing Co Ltd Transposom-aktiveret dna/rna-sekventering (ted rna-seq)
CN112359090A (zh) * 2018-08-07 2021-02-12 深圳市真迈生物科技有限公司 一种核酸片段的连接方法、测序文库的构建方法及其应用
EP3844300A1 (en) 2018-08-28 2021-07-07 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
CN112739829A (zh) * 2018-09-27 2021-04-30 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
EP4293126A3 (en) 2018-11-30 2024-01-17 Illumina, Inc. Analysis of multiple analytes using a single assay
CN112867800B (zh) * 2018-12-17 2024-07-12 Illumina公司 用于制备测序文库的方法和手段
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020210802A1 (en) * 2019-04-11 2020-10-15 University Of Virginia Patent Foundation Tagmentation to open up circles of dna and detect extrachromosomal circles of dna for diagnosis
CN110257479A (zh) * 2019-06-25 2019-09-20 北京全式金生物技术有限公司 一种快速构建rna 3’端基因表达文库的方法
AU2020312787A1 (en) 2019-07-12 2021-06-17 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
WO2021035180A1 (en) * 2019-08-22 2021-02-25 New England Biolabs, Inc. Cleavage of single stranded dna having a modified nucleotide
WO2021048444A1 (en) 2019-09-12 2021-03-18 Thermo Fisher Scientific Baltics Uab Recombinant transposon ends
CN110564705A (zh) * 2019-09-30 2019-12-13 苏州璞瑞卓越生物科技有限公司 一种用于转座酶片段化的试剂及其应用
CA3131632A1 (en) 2019-12-04 2021-06-10 Tong Liu Preparation of dna sequencing libraries for detection of dna pathogens in plasma
WO2021113505A1 (en) 2019-12-05 2021-06-10 Massachusetts Institute Of Technology Method for preparing a specimen for expansion microscopy
WO2021252617A1 (en) * 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4279609A1 (en) * 2020-12-31 2023-11-22 Beijing Institute of Genomics, Chinese Academy of Sciences (China National Center for Bioinformation) Method and kit for labeling nucleic acid molecules
EP4294933A1 (en) * 2021-02-19 2023-12-27 Nuclear RNA Networks, Inc. Compositions and methods for modulating gene transcription networks based on shared high identity transposable element remnant sequences and nonprocessive promoter and promoter-proximal transcripts
EP4314279A1 (en) 2021-03-29 2024-02-07 Illumina, Inc. Improved methods of library preparation
EP4384634A1 (en) 2021-08-11 2024-06-19 Illumina, Inc. Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
EP4341435A1 (en) 2022-03-15 2024-03-27 Illumina, Inc. Methods of base calling nucleobases
WO2024061799A1 (en) 2022-09-19 2024-03-28 Illumina, Inc. Deformable polymers comprising immobilised primers
CN115386622B (zh) * 2022-10-26 2023-10-27 北京寻因生物科技有限公司 一种转录组文库的建库方法及其应用

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
WO1995003430A1 (en) 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5523204A (en) 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
AU687535B2 (en) 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
EP0914462A4 (en) 1996-03-18 2002-05-22 Molecular Biology Resources Amplification of target nucleic acid sequences
US5773733A (en) 1996-04-12 1998-06-30 National Science Council Alumina-aluminum nitride-nickel composites
US5925545A (en) * 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
ES2320814T3 (es) 1998-11-09 2009-05-28 Eiken Kagaku Kabushiki Kaisha Procedimiento de sintesis de acido nucleico.
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6155972A (en) 1999-02-02 2000-12-05 Acorn Cardiovascular, Inc. Cardiac constraint jacket construction
EA005577B1 (ru) 1999-03-19 2005-04-28 Такара Био Инк. Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы
US6326173B1 (en) 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
AU783873B2 (en) 1999-09-13 2005-12-15 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
EP1312682A4 (en) 2000-08-23 2005-03-09 Takara Bio Inc PROCESS FOR THE MAJOR PRODUCTION OF NUCLEIC ACIDS
US7067644B2 (en) * 2000-12-05 2006-06-27 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
FR2822164B1 (fr) 2001-03-19 2004-06-18 Centre Nat Rech Scient Polypeptides derives des arn polymerases, et leurs utilisations
FI20020746A (fi) * 2002-04-18 2003-10-19 Finnzymes Oy Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi
US7108976B2 (en) * 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
WO2004027054A1 (en) 2002-09-20 2004-04-01 Prokaria Ehf. Thermostable rna ligase from thermus phage
JP2006503586A (ja) * 2002-10-28 2006-02-02 ゼオトロン コーポレイション アレイオリゴマー合成および使用
CA2506805A1 (en) 2002-11-21 2004-06-10 Epicentre Technologies Corporation Methods for using primers that encode one strand of a double-stranded promoter
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP1590477B1 (en) 2003-01-29 2009-07-29 454 Corporation Methods of amplifying and sequencing nucleic acids
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
EP1963525B2 (en) * 2005-12-02 2017-10-11 Synthetic Genomics, Inc. Synthesis of error-minimized nucleic acid molecules
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP2495337A1 (en) 2006-02-24 2012-09-05 Callida Genomics, Inc. High throughput genome sequencing on DNA arrays
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays

Also Published As

Publication number Publication date
CA2750054C (en) 2018-05-29
HK1219806A1 (zh) 2017-04-13
ES2403756T3 (es) 2013-05-21
SI2963709T1 (sl) 2017-10-30
EP2376517B1 (en) 2013-01-16
EP2664678B1 (en) 2014-10-08
CN102264914B (zh) 2015-08-12
PT2787565E (pt) 2015-11-17
CN102264914A (zh) 2011-11-30
EP3272879B1 (en) 2019-08-07
WO2010048605A1 (en) 2010-04-29
DK2963709T3 (en) 2017-09-11
ES2515065T3 (es) 2014-10-29
EP2664678A1 (en) 2013-11-20
ES2743029T3 (es) 2020-02-18
PT2963709T (pt) 2017-08-21
HK1163107A1 (en) 2012-09-07
HK1197498A1 (zh) 2015-01-16
ES2550237T3 (es) 2015-11-05
EP2376517A1 (en) 2011-10-19
JP2012506704A (ja) 2012-03-22
CA2750054A1 (en) 2010-04-29
JP5794572B2 (ja) 2015-10-14
PL2963709T3 (pl) 2017-11-30
EP2787565A1 (en) 2014-10-08
HK1169659A1 (en) 2013-02-01
DK2376517T3 (da) 2013-02-11
CN105018548A (zh) 2015-11-04
CN105018548B (zh) 2019-03-12
EP2963709B1 (en) 2017-05-24
EP2787565B1 (en) 2015-09-16
DK2787565T3 (en) 2015-10-12
HUE027961T2 (en) 2016-11-28
ES2743029T8 (es) 2020-02-27
EP2376517A4 (en) 2012-06-27
HK1249765B (zh) 2020-04-17
DK3272879T3 (da) 2019-08-26
EP2963709A1 (en) 2016-01-06
ES2637843T3 (es) 2017-10-17
EP2508529A1 (en) 2012-10-10
EP2508529B1 (en) 2013-08-28
EP3272879A1 (en) 2018-01-24
LT2963709T (lt) 2017-09-25

Similar Documents

Publication Publication Date Title
HK1217032A1 (zh) 用於修飾核酸的試劑盒
GB0706243D0 (en) Modified nucleic acids
EG25742A (en) Photobioreactor
GB0806422D0 (en) Process
GB0806419D0 (en) Process
GB0808836D0 (en) Process
EP2358913A4 (en) DETECTION PROCEDURE FOR MODIFYING RESISTANT NUCLEIC ACIDS
GB0814053D0 (en) Process
GB0803669D0 (en) Process
GB0801580D0 (en) Process
EP2309852A4 (en) NOVEL PROCEDURE
GB0807321D0 (en) Analysis of nucleic acids
GB0800875D0 (en) Process
IL201315A0 (en) Selection method for cell internalizing nucleic acids
EP2246322A4 (en) PROCESS FOR PREPARING ALPHA-FLUORO-BETA-AMINO ACIDS
GB0807161D0 (en) Process
GB0803960D0 (en) Process
GB0808764D0 (en) Process
GB0808357D0 (en) Process
GB0803663D0 (en) Process
EP2231868A4 (en) ASSAY METHOD
GB0819405D0 (en) Assay method
GB0808359D0 (en) Process
GB0807687D0 (en) Process
GB0823657D0 (en) Assay Method