ES2037273T3 - Proceso de focalizacion isoelectrica y medios para llevar a cabo dicho proceso. - Google Patents
Proceso de focalizacion isoelectrica y medios para llevar a cabo dicho proceso.Info
- Publication number
- ES2037273T3 ES2037273T3 ES198888810226T ES88810226T ES2037273T3 ES 2037273 T3 ES2037273 T3 ES 2037273T3 ES 198888810226 T ES198888810226 T ES 198888810226T ES 88810226 T ES88810226 T ES 88810226T ES 2037273 T3 ES2037273 T3 ES 2037273T3
- Authority
- ES
- Spain
- Prior art keywords
- gradients
- membranes
- isoelectric
- protein
- iselectric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
Abstract
SE DESCRIBE UN PROCESO DE ELECTROFORESIS PARA ENFOQUE ISOELECTRICO PARA LA SEPARACION Y PURIFICACION DE UNA ATMOSFERA O COMPUESTO QUIMICO NEUTRO DE UNA O MAS COMPUESTOS QUIMICOS CARGADOS ELECTRICAMENTE. DICHO PROCESO ES ILUSTRADO MAS ABAJO EN EL EJEMPLO DE LA PURIFICACION DE UNA PROTEINA DE LAS PROTEINAS Y SALES CONTAMINANTES. SE PUEDE LLEVAR A CABO EN UN APARATO ESPECIALMENTE DISEÑADO PARA ELLO, ES DECIR UN APARATO COMO SE MIUESTRA EN LA FIG 1. SE DESCRIBE DICHO APARATO Y VARIAS MODIFICACIONES DEL MISMO. LA MEZCLA A SEPARAR ESTA PRESENTE DENTRO DEL FLUJO HIDRAULICO EN LA CAMARA 8. LOS CILINDROS 5 Y 8 CONTIENE GRADIENTES DE PH INMOVILIZADOS O SON SUSTITUIDOS POR MEMBRANAS PH ISOELECTRICA ANFOTERICAS. CADA UNA DE DICHOS GRADIENTE PH Y LAS MEMBRANAS PH TIENEN CONDUCTIVIDA Y AMBOS CAPACIDAD DE TEÑIDO Y TAMPONADO EN SU INTERVALO DE PH. LAS EXTREMIDADES DE DICHOS GRADIENTES O MEMBRANAS PH QUE FORMAN EL TECHO Y EL SUEO DE LA CAMARA 8 TIENEN PUNTO ISOELECTRICOS IGUALES O MAS ALTOS Y MAS BAJOS QUELOS PUNTO ISOELECTRICOS DE LA PROTEINA DE INTERES QUE SE MANTIENE EN SU PUNTO ISOELECTRICO EN EL FLUJO HIDRAULICO Y NO ENTRA SICHAS MEMBRANAS PH Y GRADIENTES PH. CONTRARIAMENTE LAS PROTEINAS Y SALES CONTAMINANTES SON ARRASTRADAS POR UN CAMPO ELECTRICO EN DICHOS GRADIENTES DE PH O A TRAVES DE DICHOS GRADIENTES DE PH O MEMBRANAS DE PH EN LOS DEPOSITOS DE ELECTROLITO 3 Y 14. EL PROCESO DESCRITO TIENE LA VENTAJA DE QUE EL PROCESO DESEADO NO NECESITE SER DETECTADO Y EXTRAIDO DE NINGUNA MATRIZ, ES DECIR, DE DICHOS GRADIENTES DE PH, Y QUE LA RECUPERACION Y PUREZA DEL COMPUESTO DESEADO SEA MAS ALTA.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB878708746A GB8708746D0 (en) | 1987-04-11 | 1987-04-11 | Electrophoretic process |
GB878728289A GB8728289D0 (en) | 1987-04-11 | 1987-12-03 | Isoelectric focusing process & means for carrying out said process |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2037273T3 true ES2037273T3 (es) | 1993-06-16 |
Family
ID=26292133
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES198888810226T Expired - Lifetime ES2037273T3 (es) | 1987-04-11 | 1988-04-07 | Proceso de focalizacion isoelectrica y medios para llevar a cabo dicho proceso. |
Country Status (12)
Country | Link |
---|---|
US (2) | US4971670A (es) |
EP (1) | EP0287513B1 (es) |
JP (1) | JPH0781987B2 (es) |
CA (1) | CA1335805C (es) |
DE (1) | DE3876273T2 (es) |
DK (1) | DK174962B1 (es) |
ES (1) | ES2037273T3 (es) |
FI (1) | FI97893C (es) |
GR (1) | GR3007124T3 (es) |
HK (1) | HK192495A (es) |
IE (1) | IE62803B1 (es) |
PT (1) | PT87210B (es) |
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US6375899B1 (en) | 1993-11-01 | 2002-04-23 | Nanogen, Inc. | Electrophoretic buss for transport of charged materials in a multi-chamber system |
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US7857957B2 (en) * | 1994-07-07 | 2010-12-28 | Gamida For Life B.V. | Integrated portable biological detection system |
US6071394A (en) | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US6403367B1 (en) * | 1994-07-07 | 2002-06-11 | Nanogen, Inc. | Integrated portable biological detection system |
CN1051245C (zh) * | 1995-01-20 | 2000-04-12 | 清华大学 | 制备型等电点电泳分离方法及设备 |
IT1272932B (it) * | 1995-01-24 | 1997-07-01 | Pier Giorgio Righetti | Reattore ad enzima immobilizzato |
US5540826A (en) * | 1995-03-15 | 1996-07-30 | Protein Technologies, Inc. | Multi-channel separation device |
US7824532B2 (en) | 1995-04-26 | 2010-11-02 | Life Technologies Corporation | Apparatus and method for electrophoresis |
JP4051421B2 (ja) * | 1995-07-05 | 2008-02-27 | サノフイ−アベンテイス・エツセ・ピー・アー | 等電点電気泳動によるダルバヘプチド抗生物質の精製 |
DE19831210A1 (de) * | 1998-07-03 | 2000-01-05 | Wita Gmbh Wittmann Inst Of Tec | Verfahren und Vorrichtung zur zwei-dimensionalen Trennung von Biomolekülen |
AUPP521298A0 (en) * | 1998-08-12 | 1998-09-03 | Life Therapeutics Limited | Purification of fibrinogen |
SE9803224D0 (sv) * | 1998-09-23 | 1998-09-23 | Amersham Pharm Biotech Ab | Method for separation of macromolecules |
US20050224355A1 (en) * | 1999-12-23 | 2005-10-13 | Brendon Conlan | Removal of biological contaminants |
AUPP790898A0 (en) | 1998-12-23 | 1999-01-28 | Life Therapeutics Limited | Renal dialysis |
AUPP790698A0 (en) | 1998-12-23 | 1999-01-28 | Life Therapeutics Limited | Separation of microorganisms |
US6267579B1 (en) * | 1998-12-23 | 2001-07-31 | Clinical Laboratory Development Group, Inc. | Apparatus for making a gradient gel |
AUPP971399A0 (en) | 1999-04-12 | 1999-05-06 | Life Therapeutics Limited | Separation of plasma components |
US6284115B1 (en) | 1999-09-21 | 2001-09-04 | Agilent Technologies, Inc. | In-line flow through micro-dialysis apparatus and method for high performance liquid phase separations |
US6758953B2 (en) | 1999-10-28 | 2004-07-06 | Nathan A. Thomas | Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes |
JP2003514829A (ja) * | 1999-11-15 | 2003-04-22 | プロテオム システムズ リミテッド | 多室電気泳動 |
WO2001036071A1 (en) * | 1999-11-16 | 2001-05-25 | Champagne James T | Solution based two-dimensional separation and detection of amphoteric substances |
US7077942B1 (en) | 1999-12-23 | 2006-07-18 | Gradipore Limited | Removal of biological contaminants |
US20080096284A1 (en) * | 2000-02-08 | 2008-04-24 | Regents Of The University Of Michigan | Protein separation and analysis |
AU3812000A (en) * | 2000-03-15 | 2001-09-24 | Proteosys Ag | Micropreparative isoelectric focussing |
US6638408B1 (en) * | 2000-04-03 | 2003-10-28 | The Wistar Institute | Method and device for separation of charged molecules by solution isoelectric focusing |
AUPQ691400A0 (en) * | 2000-04-14 | 2000-05-11 | Life Therapeutics Limited | Separation of micromolecules |
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ATE449200T1 (de) | 2000-04-18 | 2009-12-15 | Gradipore Ltd | Trennung und behandlung von proben durch elektrophorese |
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US6562213B1 (en) * | 2000-08-30 | 2003-05-13 | Ethrog Biotechnology Ltd. | Electrophoresis apparatus for simultaneous loading of multiple samples |
US6923896B2 (en) * | 2000-09-22 | 2005-08-02 | The Texas A&M University System | Electrophoresis apparatus and method |
AUPR051500A0 (en) | 2000-09-29 | 2000-10-26 | Proteome Systems Ltd | Electrophoresis system |
DE60142520D1 (de) * | 2000-10-06 | 2010-08-19 | Arturus Capital Ltd | Multi-port-elektrophorese-trennvorrichtung und entsprechendes verfahren |
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WO2003008977A2 (en) * | 2001-07-16 | 2003-01-30 | Protein, Forest, Inc. | Arrays of buffers for analysing biomolecules by their isoelectric point |
GB0121189D0 (en) * | 2001-08-31 | 2001-10-24 | Diagnoswiss Sa | Apparatus and method for separating an analyte |
US6887362B2 (en) * | 2002-02-06 | 2005-05-03 | Nanogen, Inc. | Dielectrophoretic separation and immunoassay methods on active electronic matrix devices |
ES2558303T3 (es) * | 2002-09-11 | 2016-02-03 | Chugai Seiyaku Kabushiki Kaisha | Método de purificación de proteínas |
US20060134620A1 (en) * | 2002-11-28 | 2006-06-22 | Mitsuharu Hirai | Method and apparatus for concentration and purification of nucleic acid |
US7622028B2 (en) * | 2003-05-09 | 2009-11-24 | Life Technologies Corporation | Solution phase electrophoresis device, components, and methods |
US7850835B2 (en) * | 2003-05-09 | 2010-12-14 | Life Technologies Corporation | Solution phase electrophoresis device, components, and methods |
US7459021B2 (en) * | 2003-09-03 | 2008-12-02 | Shmuel Bukshpan | Methods and apparatus for rapid crystallization of biomolecules |
EP1671112A4 (en) * | 2003-10-07 | 2011-03-23 | Life Technologies Corp | IMPROVED GELE FOR ISOELECTRIC FOCUSING AND METHOD FOR USE THEREOF |
EP1918018A3 (en) * | 2003-11-10 | 2009-03-11 | Arkray, Inc. | Apparatus for concentrating and purifying nucleic acid |
US20050197496A1 (en) * | 2004-03-04 | 2005-09-08 | Gtc Biotherapeutics, Inc. | Methods of protein fractionation using high performance tangential flow filtration |
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US20060130159A1 (en) * | 2004-12-09 | 2006-06-15 | Nick Masiello | Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum |
WO2006127056A2 (en) | 2005-05-25 | 2006-11-30 | Bio-Rad Laboratories, Inc. | Fluidics device |
CA2616976C (en) * | 2005-07-28 | 2012-08-28 | Bio-Rad Laboratories, Inc. | Separation of proteins based on isoelectric point using solid-phase buffers |
US7531632B2 (en) * | 2006-02-16 | 2009-05-12 | Gtc Biotherapeutics, Inc. | Clarification of transgenic milk using depth filtration |
AU2007243994B2 (en) | 2006-04-27 | 2012-08-02 | Ge Healthcare Bio-Sciences Ab | Electrodic bridge |
US20080220442A1 (en) * | 2006-12-06 | 2008-09-11 | Proteinics | Difference detection methods using isoelectric focusing chips |
US8366899B2 (en) * | 2007-06-22 | 2013-02-05 | Massachusetts Institute Of Technology | Isoelectric focusing systems and methods |
AU2008310248A1 (en) * | 2007-10-09 | 2009-04-16 | Dalhousie University | Apparatus for purifying molecules |
US8282803B2 (en) | 2009-03-25 | 2012-10-09 | Bio-Rad Laboratories, Inc. | Isoelectric focusing tray and electrode assembly for alternate gel strip orientations |
WO2013067509A1 (en) | 2011-11-04 | 2013-05-10 | Bio-Rad Laboratories, Inc. | Simultaneous purification of cell components |
US9766207B2 (en) | 2011-11-04 | 2017-09-19 | Bio-Rad Laboratories, Inc. | Affinity methods and compositions employing electronic control of pH |
US20130164860A1 (en) * | 2011-11-04 | 2013-06-27 | Technion Research & Development Foundation Ltd. | Affinity methods and compositions employing electronic control of ph |
WO2013078236A1 (en) | 2011-11-22 | 2013-05-30 | Haralampu Stephen G | Stopped-flow, micro-fluidic device and method for the charge-based separation of complex analyte mixtures |
CN104254390B (zh) | 2012-02-15 | 2017-05-10 | 生物辐射实验室股份有限公司 | pH和离子强度的电子控制 |
US9321012B2 (en) | 2012-04-04 | 2016-04-26 | Bio-Rad Laboratories, Inc. | Electronic protein fractionation |
US9671368B2 (en) * | 2013-05-10 | 2017-06-06 | The Regents Of The University Of California | Two-dimensional microfluidic devices and methods of using the same |
TW201509432A (zh) | 2013-07-05 | 2015-03-16 | Lab Francais Du Fractionnement | 親和層析基質 |
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Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3539493A (en) * | 1967-08-31 | 1970-11-10 | Canal Ind Corp | Apparatus for preparative electrophoresis on gel support media |
US3844925A (en) * | 1973-07-02 | 1974-10-29 | Center For Blood Res | Molecular fractionation |
SE415731B (sv) * | 1977-04-26 | 1980-10-27 | Pharmacia Fine Chemicals Ab | Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma |
WO1979000002A1 (en) * | 1977-06-15 | 1979-01-11 | Nat Res Dev | Improvements relating to membrane electrophoresis |
EP0103965A2 (en) * | 1982-08-20 | 1984-03-28 | Imperial Chemical Industries Plc | Electrofocusing apparatus |
US5114555A (en) * | 1988-01-05 | 1992-05-19 | Monsanto Company | Continuous isoelectric separation |
-
1988
- 1988-04-07 DE DE8888810226T patent/DE3876273T2/de not_active Expired - Lifetime
- 1988-04-07 EP EP88810226A patent/EP0287513B1/en not_active Expired
- 1988-04-07 ES ES198888810226T patent/ES2037273T3/es not_active Expired - Lifetime
- 1988-04-08 CA CA000563616A patent/CA1335805C/en not_active Expired - Lifetime
- 1988-04-08 IE IE106788A patent/IE62803B1/en not_active IP Right Cessation
- 1988-04-08 US US07/179,619 patent/US4971670A/en not_active Expired - Lifetime
- 1988-04-08 PT PT87210A patent/PT87210B/pt not_active IP Right Cessation
- 1988-04-08 FI FI881652A patent/FI97893C/fi not_active IP Right Cessation
- 1988-04-08 DK DK198801913A patent/DK174962B1/da not_active IP Right Cessation
- 1988-04-08 JP JP63085487A patent/JPH0781987B2/ja not_active Expired - Lifetime
-
1990
- 1990-09-04 US US07/577,421 patent/US5082548A/en not_active Expired - Lifetime
-
1993
- 1993-02-23 GR GR920402737T patent/GR3007124T3/el unknown
-
1995
- 1995-12-21 HK HK192495A patent/HK192495A/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE3876273T2 (de) | 1993-05-27 |
FI97893B (fi) | 1996-11-29 |
CA1335805C (en) | 1995-06-06 |
JPS63263457A (ja) | 1988-10-31 |
DK191388D0 (da) | 1988-04-08 |
IE62803B1 (en) | 1995-03-08 |
GR3007124T3 (es) | 1993-07-30 |
DK174962B1 (da) | 2004-03-29 |
FI881652A (fi) | 1988-10-12 |
PT87210B (pt) | 1993-11-30 |
PT87210A (pt) | 1989-05-12 |
DE3876273D1 (de) | 1993-01-14 |
EP0287513A2 (en) | 1988-10-19 |
EP0287513A3 (en) | 1990-10-10 |
EP0287513B1 (en) | 1992-12-02 |
DK191388A (da) | 1988-10-12 |
FI97893C (fi) | 1997-03-10 |
US4971670A (en) | 1990-11-20 |
HK192495A (en) | 1995-12-29 |
IE881067L (en) | 1988-10-11 |
US5082548A (en) | 1992-01-21 |
FI881652A0 (fi) | 1988-04-08 |
JPH0781987B2 (ja) | 1995-09-06 |
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