EP1558933A1 - Detection electronique de molecules biologiques fondee sur des nanotubes - Google Patents
Detection electronique de molecules biologiques fondee sur des nanotubesInfo
- Publication number
- EP1558933A1 EP1558933A1 EP03768779A EP03768779A EP1558933A1 EP 1558933 A1 EP1558933 A1 EP 1558933A1 EP 03768779 A EP03768779 A EP 03768779A EP 03768779 A EP03768779 A EP 03768779A EP 1558933 A1 EP1558933 A1 EP 1558933A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- nanotube
- compound
- ntfet
- sensor
- hydrophilic polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
Definitions
- the present invention relates to the detection of biological molecules by nanotube-based sensors.
- Nanowires and nanotubes by virtue of their small size, large surface area, and near one-dimensionality of electronic transport, are promising candidates for electronic detection of chemical and biological species (1 ).
- Field effect transistors (FETs) fabricated from component semiconducting single wall carbon nanotubes (NTs) have been studied extensively for their potential as sensors. A number of properties of these devices have been identified, and different mechanisms have been proposed to describe their sensing behavior. Devices that incorporate carbon nanotubes have been found to be sensitive to various gases, such as oxygen and ammonia, and these observations have confirmed the notion that such devices can operate as sensitive chemical sensors.
- SWNT devices including field-effect transistors (“FET's”) and resistors
- FET's field-effect transistors
- resistors can be fabricated using nanotubes grown on silicon or other substrates by chemical vapor deposition from iron-containing catalyst nanoparticles with methane/hydrogen gas mixture at 900°C.
- Other catalyst materials and gas mixtures can be used to grow nanotubes on substrates, and other electrode materials and nanostructure configurations and have been described previously by Gabriel et al. in U.S. Patent Application No. 10/099,664 and in U.S. Patent Application No. 10/177,929, both of which are incorporated by reference herein.
- Currently, technology for constructing practical nanostructure devices is in its infancy. While nanotube structures show promise for use as sensor devices and transistors, current technology is limited in many ways.
- a useful sensor of this type should selectively and reliably respond to a molecular target of a specific type. For example, it may be desirable to selectively sense a specific protein, while not responding to the presence of other proteins in the sample.
- Examples of covalent chemical attachment of biological molecules to nanotubes, including proteins and DNA, are known in the art, although it has not been convincingly demonstrated that useful detection of specific proteins or other large biomolecules can be accomplished in this way.
- covalent chemical attachment has the disadvantage of impairing physical properties of carbon nanotubes, making structures of this type less useful as practical sensors.
- the carbon nanotubes are hydrophobic, and generally non-selective in reacting with biomolecules.
- nanotube sensing device that is biocompatible, and exhibits a high degree of selectivity to particular biomolecular targets.
- a nanotube sensor architecture which allows the detection of protein-protein interactions and, at the same time, reduces or eliminates non-specific binding.
- the sensor may be operated as a nanostructure field effect transistor, to detect the presence of a specific protein or other biomolecule. Further provided are methods for making and operating the sensing device.
- a nanostructure device may comprise a nanotube, such as a carbon nanotube, disposed along a substrate, such as a silicon substrate.
- the nanotube may span two conductive elements, which may serve as electrical terminals, or as a source and drain.
- a passivation layer such as of silicon monoxide, may be deposited over the conductive elements and a portion of the nanotube, leaving a portion of the nanotube between the conductive elements exposed.
- the nanotube may be coated with a thin polymer layer, for example comprising poly(ethylene imine) (“PEI”) and poly(ethylene glycol) (PEG).
- PEI poly(ethylene imine)
- PEG poly(ethylene glycol)
- the device may be operated as an n-type FET, as further described in Application Serial No. 10/656,898.
- the polymer layer is hydrophilic and biocompatible, making the nanotube device essentially non-reactive to large biomolecules such as proteins.
- a bioreceptor layer may be attached over the polymer layer, configured for reactivity to a specific biomolecule.
- biotin is known to selectively bind to streptavidin.
- the bioreceptor layer should be configured to bind to the polymer layer.
- a solution of biotin-N-hydroxysuccinimide ester reacts with primary amines in PEI, thereby binding biotin molecules to the polymer layer.
- the bioreceptor layer may comprise a mono-molecular layer, comprised of discrete bioreceptor molecules attached to the polymer layer.
- the resulting device will exhibit transconductance that varies depending on the presence of the targeted biomolecule in its sample environment.
- a bioreceptor layer comprised of attached biotin molecules will selectively bind to streptavidin, causing a measurable decrease in transconductance at negative gate voltages.
- the device may therefore be used as a sensor for streptavidin.
- the device may be provided with a different bioreceptor layer that is configured to bind to the desired target.
- Fig. 1. is a schematic diagram of a nanotube field effect transistor (NTFET) configured as a biomolecule sensor according to the invention.
- NFET nanotube field effect transistor
- Fig. 2 is a flow chart showing exemplary steps of a method for making a nanotube biosensor according to the invention.
- Fig. 3A is a schematic of a chemical scheme for bonding biotin to a PEI/PEG polymer layer over a nanotube.
- Fig. 3B is a chart comparing transconductance of a native PEI/PEG-coated NTFET device with its transconductance after 1 hour, and after 18 hours of being reacted with biotin-N-hydroxysuccinimide ester.
- Fig. 4 is a chart comparing transconductance of a bare NTFET to a NTFET coated with a PEI/PEG polymer layer and a NTFET with a biotinylated PEI/PEG layer.
- Fig. 5 is a chart comparing the transconductance of a biotinylated, PEI/PEG- coated NTFET device, in the absence and presence of streptavidin.
- Fig. 6 is a chart comparing the transconductance of a bare NTFET device, in the absence and presence of streptavidin.
- Fig. 7 is a chart comparing the transconductance of a PEI/PEG-coated NTFET device without biotin receptors, in the absence and presence of streptavidin.
- Fig. 8 is a chart comparing the transconductance of a biotinylated, PEI/PEG- coated NTFET device, in the absence and presence of streptavidin that has been complexated with biotin, thereby blocking its binding sites.
- the present invention provides a nanotube sensor to selectively sense biological molecules, that overcomes the limitations of the prior art. These advancements have been demonstrated by a nanotube sensor according to the invention, which has been shown to be selectively sensitive to the well-characterized ligand-receptor binding of biotin-streptavidin.
- the invention provides a sensor architecture that allows the detection of protein-protein interactions, and also reduces or eliminates non-specific binding.
- An inherently hydrophobic NT-FET covered with a polymer coating layer with hydrophilic properties, is used as a transducer.
- the hydrophilicity of the polymer layer reduces the affinity of nanotubes towards non-specific protein binding, which is favored by a hydrophobic environment.
- biotin is covalently attached to the polymer. When in use, the attached biotin binds with the complementary protein streptavidin, and the formation of the streptavidin-biotin complex is electronically detectable.
- Fig. 1 schematically depicts a sensor 100 that uses a carbon nanotube 102 as a transducer. Nanotube 102 is covered with a polymer coating 104 that has hydrophilic properties and onto which a bioreceptor molecule 106 is attached by a chemical bond to the underlying layer. Bioreceptor 106 may be selected for its selectivity in binding to a biomolecule target 107. Various receptor/target combinations are known, or may be discovered. In an embodiment, the receptor 106 is biotin, and the target 107 is streptavidin.
- Additional bioreceptor molecules of the same or different types as molecule 106 may additionally be attached to polymer layer 104.
- a plurality of such bioreceptor molecules may disposed over the surface of the polymer layer.
- the nanotube 102 may be connected to a source electrode 108 and a drain electrode 110 on gate 112.
- a passivation layer 114 as known in the art, such as SiO 2 may cover the gate substrate 112, which may comprise a silicon or other suitable material.
- Functionalization via polymer layer 104 in this sensor architecture has several advantages.
- the polymer is used to attach molecular receptor molecules to the sidewalls of nanotubes, thereby avoiding covalent chemical attachment of biological molecules to nanotubes.
- polymer coatings have been shown to modify the characteristics of nanotube FET devices, and thus the coating process can be readily monitored.
- coating NTFETs with polyethylene imine (PEI) polymer advantageously shifts the device characteristic from p- to n-type.
- the polymer coating may be useful for preventing nonspecific binding of proteins.
- PEI poly(ethylene imine)
- PEG poly(ethylene glycol)
- Attachment of a bioreceptor, such as biotin, to PEI is through covalent binding to the primary NH 2 group, which would be expected to reduce the overall electron donating function of PEI and cause a transconductance profile that is consistent with indicating removal of electrons from the device.
- the primary NH 2 sites are involved in binding to biotin, the p-type conductance observed before coating is not fully recovered. It is reasonable to postulate that upon streptavidin-biotin binding, geometric changes occur which locally perturb the coating, thereby reducing the effectiveness of the charge transfer and altering the transconductance of the device. It is worth noting that functionalization via the primary NH 2 group of the PEI or other polymer layer could be applied to oligonucleotides, as well as to proteins.
- layer 104 may reduce the affinity of nanotubes toward protein binding and thereby improve the selectivity of the device.
- a variety of polymer coatings and self-assembled mono-molecular layers have been used to prevent binding of undesired species on surfaces for biosensor and biomedical device applications, and may also be suitable for use with the invention.
- poly(ethylene glycol) is one of the most effective and widely used.
- a p-type NTFET may be fabricated using nanotubes grown by chemical vapor deposition (CVD) on 200 nm of silicon dioxide on doped silicon from iron nanoparticles with methane/hydrogen gas mixture at 900 °C. Electrical leads may be patterned on top of the nanotubes from titanium films 35 nm thick capped with gold layers 5 nm thick, with a gap of 0.5 to 0.75 ⁇ m between source and drain. Multiple nanotubes may be connected to the source and drain electrodes, with the individual tubes varying from metallic to semiconducting.
- CVD chemical vapor deposition
- Exemplary devices resulting from the foregoing process may have 0.5 ⁇ m wide pairs of electrical leads separated by 0.5 to 0.75 ⁇ m gaps, and these gaps may be bridged by 1 to about 5 nanotubes along a 10 ⁇ m length of a pair of leads. It should be apparent that numerous other configurations may also be suitable.
- the device characteristic for the NTFET may be determined.
- device characteristic refers to the dependence of the source-drain current, l sd , as function of the gate voltage V g , l Sd (V g ), measured from +10 V to -10 V. Any other suitable measure may also be used to characterize the NTFET device.
- the device characteristic may be used later as a baseline for subsequent calibration of the device's electrical response. '
- a polymer functionalization layer may be deposited over the device at step 206.
- the device may be submerged in a 10 wt % solution of poly(ethylene imine) (PEI, average molecular weight -25 000, Aldrich) and poly(ethylene glycol) (PEG, average molecular weight 10 000, Aldrich) in water overnight, followed by thorough rinsing with water.
- PEI poly(ethylene imine)
- PEG poly(ethylene glycol)
- the desired biomolecular receptor may be bonded to the polymer layer.
- a polymer-coated device may be biotinylated by submerging in a 15 mM DMF solution of biotin-N-hydroxysuccinimide ester (Sigma) at room temperature. This compound readily reacts with primary amines in PEI under ambient conditions, leading to changes of the device characteristic as will be discussed below. After soaking overnight, devices may be removed from solution, rinsed with DMF and deionized water, blown dry in nitrogen flow, and dried in a vacuum.
- Fig. 3A depicts a chemical scheme by which biotin may be attached to the polymer coating.
- Fig. 3B shows an exemplary transconductance curve for a PEI-coated device prior to the biotinylating reaction, and after 1 hour and 18 hours, respectively, of the reaction.
- the device characteristics may be examined after drying, as reported herein. While the device may also exhibit a response in a buffer or other fluid, the examples herein should serve to illustrate the changes of the device characteristic, brought about by different chemical and biological modifications. Such direct correspondence may be somewhat obscured in a buffer environment. Illustrative results are reported below. After drying, biotinylated polymer-coated devices constructed according to the foregoing description were exposed to a 2.5 ⁇ M solution of streptavidin 15 in 0.01 M phosphate buffered saline (pH ) 7.2, Sigma) at room temperature for 15 min. Subsequently, the devices were thoroughly rinsed with deionized water and blown dry with nitrogen.
- the imaged device comprised a nanotube about 800 nm long, and approximately 80 streptavidin molecules were surmised to be in direct interaction with the nanotube conducting channel.
- the device characteristic of the sensor before chemical modification was p-type in an ambient environment, presumably due to exposure to oxygen. Coating the device with the mixture of PEI and PEG polymers resulted in an n-type device characteristic, as shown by Fig. 4. The electronic characteristic of the device after 18 h of biotinylation reaction is also depicted in Fig. 4. Note that the p-type conductance observed before coating with PEI is not fully recovered after functionalization with biotin.
- label-free electronic sensing with a nanotube based transducer as the central sensor element may provide other significantly useful features in the detection of biological molecules.
- Such sensors are small, fast, require very little power, and thus generate little heat.
- the active sensing area is sized for individual proteins or viruses, and small sample volume in general, and is extremely sensitive as all the current passes through the detection point.
- devices can be made specific to individual molecules, and potentially their response to different molecules can be controlled by using chemical and biological functionalization. Direct detection of specific oligonucleotides, in some ways, is typically even more challenging, and thus represents information more valuable than that of detecting individual proteins.
- Oligonucleotides in a sample generally show a high degree of variation, based on sequence, and often species of particular interest are rare from two perspectives, as a sample can contain populations of many oligonucleotide species very similar to the ones of interest, and at much higher concentrations.
- the principles and practice of the invention may contribute, in due course, to the development of cell-based electronic sensing: measuring the electronic response of living systems, and to using nanoscale devices for in-vivo applications directed toward cellular physiology, medical screening, and diagnosis.
- Sensor devices may be constructed, according to the principles of the invention, wherein surface charges can be created on the sensing element when the biological molecules are immobilized, by applying a voltage between elements of the sensor. Such surface charges should interact with the charged bio-molecules, providing further opportunities for selective electronic detection of biomolecules, or electrical manipulation of biological reactions at a molecular level. Operation of a device according to the invention in this manner may therefore merit further study.
Abstract
L'invention concerne des dispositifs de transistor à effet de champ conçus à l'échelle nanométrique, qui comprenent des nanotubes de carbone en tant que canal de conduction et sont utilisés pour détecter des liaisons protéine-protéine. Un revêtement constitué d'un polymère donneur d'électrons est appliqué sur un dispositif de nanotube, et un composé récepteur est lié audit polymère. Ce composé récepteur est configuré pour se lier à une/des molécule(s) biologique(s) spécifique(s). Le dispositif recouvert du revêtement polymère et du composé récepteur peut fonctionner à la manière d'un transducteur à effet de champ du type p. Par exemple, un signal tel que la présence de molécules biologiques auxquelles le composé recépteur se lie peut entraîner une réduction significative de la conductance à une tension négative, ce qui crée une réponse électronique audit signal.
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
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US345783 | 1989-05-01 | ||
US42489202P | 2002-11-08 | 2002-11-08 | |
US424892P | 2002-11-08 | ||
US10/345,783 US20030134433A1 (en) | 2002-01-16 | 2003-01-16 | Electronic sensing of chemical and biological agents using functionalized nanostructures |
US656898 | 2003-09-05 | ||
US10/656,898 US20050279987A1 (en) | 2002-09-05 | 2003-09-05 | Nanostructure sensor device with polymer recognition layer |
PCT/US2003/035635 WO2004044586A1 (fr) | 2002-11-08 | 2003-11-07 | Detection electronique de molecules biologiques fondee sur des nanotubes |
Publications (1)
Publication Number | Publication Date |
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EP1558933A1 true EP1558933A1 (fr) | 2005-08-03 |
Family
ID=36337451
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03768779A Withdrawn EP1558933A1 (fr) | 2002-11-08 | 2003-11-07 | Detection electronique de molecules biologiques fondee sur des nanotubes |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1558933A1 (fr) |
JP (1) | JP2006505806A (fr) |
AU (1) | AU2003291385A1 (fr) |
WO (1) | WO2004044586A1 (fr) |
Cited By (2)
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US7522040B2 (en) | 2004-04-20 | 2009-04-21 | Nanomix, Inc. | Remotely communicating, battery-powered nanostructure sensor devices |
US7547931B2 (en) | 2003-09-05 | 2009-06-16 | Nanomix, Inc. | Nanoelectronic capnometer adaptor including a nanoelectric sensor selectively sensitive to at least one gaseous constituent of exhaled breath |
Families Citing this family (27)
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---|---|---|---|---|
US8154093B2 (en) | 2002-01-16 | 2012-04-10 | Nanomix, Inc. | Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices |
US7714398B2 (en) * | 2002-09-05 | 2010-05-11 | Nanomix, Inc. | Nanoelectronic measurement system for physiologic gases and improved nanosensor for carbon dioxide |
US20050129573A1 (en) * | 2003-09-12 | 2005-06-16 | Nanomix, Inc. | Carbon dioxide nanoelectronic sensor |
US7955483B2 (en) * | 2002-03-18 | 2011-06-07 | Honeywell International Inc. | Carbon nanotube-based glucose sensor |
US7948041B2 (en) | 2005-05-19 | 2011-05-24 | Nanomix, Inc. | Sensor having a thin-film inhibition layer |
WO2005043160A2 (fr) * | 2003-10-31 | 2005-05-12 | University Of Hawaii | Plate-forme de detection ultrasensible d'agents biochimiques |
US7247877B2 (en) * | 2004-08-20 | 2007-07-24 | International Business Machines Corporation | Integrated carbon nanotube sensors |
WO2006024023A2 (fr) * | 2004-08-24 | 2006-03-02 | Nanomix, Inc. | Dispositifs de detection a nanotubes, destines a la detection de sequences d'adn |
WO2006052891A1 (fr) * | 2004-11-09 | 2006-05-18 | The Regents Of The University Of California | Identification d'analytes au moyen de dispositifs electroniques |
EP1831670A4 (fr) * | 2004-12-28 | 2010-09-15 | Nanomix Inc | Dispositifs nanoelectroniques pour la detection d'adn et la reconnaissance de sequences polynucleotidiques |
DE102005011012A1 (de) * | 2005-03-10 | 2006-09-14 | Steinbeis GmbH & Co. für Technologietransfer Zell-Chip-Technologien | Chemischer Sensor sowie Verfahren zu dessen Herstellung |
US7776269B2 (en) * | 2005-03-15 | 2010-08-17 | The United States Of America As Represented By The Secretary Of The Navy | Capacitive based sensing of molecular adsorbates on the surface of single wall nanotubes |
JP4742650B2 (ja) * | 2005-04-08 | 2011-08-10 | 東レ株式会社 | カーボンナノチューブ組成物、バイオセンサーおよびそれらの製造方法 |
KR100748408B1 (ko) * | 2005-06-28 | 2007-08-10 | 한국화학연구원 | 압타머를 이용한 탄소 나노튜브 트랜지스터 바이오센서 및이것을 이용한 타겟물질 검출 방법 |
WO2007109228A1 (fr) * | 2006-03-17 | 2007-09-27 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Appareil de capteurs liés en microréseau incluant des matériaux pour une sonde biologique et utilisant des transistors en nanotubes de carbone |
KR100842886B1 (ko) * | 2006-04-04 | 2008-07-02 | 재단법인서울대학교산학협력재단 | 나노선을 이용한 식품 첨가물 l-글루타민산나트륨 검출용바이오센서 및 이의 제조 방법 |
KR100758285B1 (ko) * | 2006-09-27 | 2007-09-12 | 한국전자통신연구원 | 바이오 센서, 그 제조방법 및 이를 구비한 바이오 감지장치 |
JP2008116356A (ja) * | 2006-11-06 | 2008-05-22 | Nec Corp | 観測装置および観測方法 |
CA2701380C (fr) * | 2007-10-01 | 2014-03-11 | University Of Southern California | Detection d'adn methyle et de mutations d'adn |
FI122511B (fi) * | 2009-02-26 | 2012-02-29 | Valtion Teknillinen | Grafeenia sisältävät hiutaleet ja menetelmä grafeenin eksfoliaatiota varten |
JP2012529056A (ja) * | 2009-06-05 | 2012-11-15 | アリゾナ・ボード・オブ・リージェンツ・アクティング・フォー・アンド・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティ | 気体試料中の酸化窒素用の集積型光電気化学センサ |
KR101040083B1 (ko) | 2009-07-27 | 2011-06-09 | 전자부품연구원 | 나노와이어 트랜지스터 센서, 제조방법 및 이를 이용한 생체분자 검출장치 |
US8993346B2 (en) | 2009-08-07 | 2015-03-31 | Nanomix, Inc. | Magnetic carbon nanotube based biodetection |
JP6076738B2 (ja) * | 2009-09-11 | 2017-02-08 | ジェイピー ラボラトリーズ インコーポレイテッド | ナノ構造の変形、破壊、および変換に基づくモニタリング装置およびモニタリング方法 |
KR101263188B1 (ko) * | 2011-04-14 | 2013-05-10 | 포항공과대학교 산학협력단 | 네트워크 구조의 나노선을 구비한 나노선 센서 및 그 제조방법 |
KR102068087B1 (ko) * | 2017-03-24 | 2020-01-20 | 가천대학교 산학협력단 | 나노와이어 전계효과 트랜지스터 기반의 인슐린 검출 센서 |
JP6857353B2 (ja) * | 2017-03-30 | 2021-04-14 | 国立大学法人東北大学 | 生体分子センサー |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100469868B1 (ko) * | 1996-03-06 | 2005-07-08 | 하이페리온 커탤리시스 인터내셔널 인코포레이티드 | 작용화된나노튜브 |
WO2001044796A1 (fr) * | 1999-12-15 | 2001-06-21 | Board Of Trustees Of The Leland Stanford Junior University | Dispositifs de nanotubes de carbone |
KR100991573B1 (ko) * | 2000-12-11 | 2010-11-04 | 프레지던트 앤드 펠로우즈 오브 하버드 칼리지 | 나노센서 |
WO2002079514A1 (fr) * | 2001-01-10 | 2002-10-10 | The Trustees Of Boston College | Reseaux de nanotubes de carbone a pont d'adn |
US8029734B2 (en) * | 2001-03-29 | 2011-10-04 | The Board Of Trustees Of The Leland Stanford Junior University | Noncovalent sidewall functionalization of carbon nanotubes |
KR100455284B1 (ko) * | 2001-08-14 | 2004-11-12 | 삼성전자주식회사 | 탄소나노튜브를 이용한 고용량의 바이오분자 검출센서 |
US20030134433A1 (en) * | 2002-01-16 | 2003-07-17 | Nanomix, Inc. | Electronic sensing of chemical and biological agents using functionalized nanostructures |
-
2003
- 2003-11-07 WO PCT/US2003/035635 patent/WO2004044586A1/fr active Search and Examination
- 2003-11-07 JP JP2005507121A patent/JP2006505806A/ja active Pending
- 2003-11-07 EP EP03768779A patent/EP1558933A1/fr not_active Withdrawn
- 2003-11-07 AU AU2003291385A patent/AU2003291385A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
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See references of WO2004044586A1 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7547931B2 (en) | 2003-09-05 | 2009-06-16 | Nanomix, Inc. | Nanoelectronic capnometer adaptor including a nanoelectric sensor selectively sensitive to at least one gaseous constituent of exhaled breath |
US7522040B2 (en) | 2004-04-20 | 2009-04-21 | Nanomix, Inc. | Remotely communicating, battery-powered nanostructure sensor devices |
Also Published As
Publication number | Publication date |
---|---|
WO2004044586A8 (fr) | 2005-07-07 |
JP2006505806A (ja) | 2006-02-16 |
WO2004044586A1 (fr) | 2004-05-27 |
AU2003291385A1 (en) | 2004-06-03 |
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