EP0711603A1 - Système pour l'incubation des échantillons liquides - Google Patents

Système pour l'incubation des échantillons liquides Download PDF

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Publication number
EP0711603A1
EP0711603A1 EP95117563A EP95117563A EP0711603A1 EP 0711603 A1 EP0711603 A1 EP 0711603A1 EP 95117563 A EP95117563 A EP 95117563A EP 95117563 A EP95117563 A EP 95117563A EP 0711603 A1 EP0711603 A1 EP 0711603A1
Authority
EP
European Patent Office
Prior art keywords
incubation
vessels
holding plate
block
rack
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP95117563A
Other languages
German (de)
English (en)
Other versions
EP0711603B1 (fr
Inventor
Volker Degenhardt
Manfred Dr. Böhm
Alois Rainer
Albert Wohland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH, Boehringer Mannheim GmbH filed Critical Roche Diagnostics GmbH
Publication of EP0711603A1 publication Critical patent/EP0711603A1/fr
Application granted granted Critical
Publication of EP0711603B1 publication Critical patent/EP0711603B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/809—Incubators or racks or holders for culture plates or containers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/809—Multifield plates or multicontainer arrays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/81—Tube, bottle, or dipstick
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Definitions

  • the known devices can basically be divided into two classes.
  • the first class includes incubators in which the sample vessels are heated by a fluid phase.
  • Devices of this type are described, for example, in EP-A-0 363 143 and EP-B-0 087 028.
  • devices are described in which sample vessels are located in a holder and are immersed in a liquid with this holder. The temperature of the sample vessels or sample liquids is controlled by the liquid inside the incubator. Due to the fluid properties of the liquid phase, the liquid adapts perfectly to the shape of the incubation vessels.
  • the second class of incubation devices includes so-called metal block incubators in which there are bores within an incubation block made of heat-conducting material, which serve to hold incubation vessels.
  • incubation devices are known for example from EP-A-0 151 781 or US-4,335,620. Reference is hereby made in full to the two documents mentioned.
  • US 4,335,620 describes an incubator which has a solid block made of a thermally conductive material such as e.g. B. aluminum.
  • the block serves both to hold sample vessels and as a heat sink for temperature control of the vessels.
  • the document relates to a special design for thermally isolating the device from the environment. The total heat loss of the equipment is minimized.
  • Document US-4,727,034 relates to a device for thermostatting sample liquids.
  • the vessels with sample liquids are placed in a rack made of a good heat-conductive material.
  • the rack in turn is clamped in the device between two side walls, at least one of which is heated, so that the temperature of the rack and thus the sample liquids in the vessels is possible.
  • the object of the invention was to eliminate this disadvantage of metal block incubators and to propose an incubation device which combines the advantages of metal block incubators and fluid incubators.
  • Sample liquids for incubation are to be understood as meaning blood samples, serum samples, urine, food samples, water samples, reaction batches and the like.
  • those liquids are also included which are obtained from sample materials by adding reagents.
  • DNA-containing samples should also be included, which are mixed with reagents to amplify the DNA.
  • a system according to the invention can be used in particular for chemical and clinical chemical analysis, since in this area the maintenance of certain temperatures or the pretreatment of samples at certain temperatures is of crucial importance for the reliability of the analysis results.
  • a system for incubation according to the invention can also be used, for example, as a so-called thermal cycler, which is used in the amplification of DNA.
  • Incubation is to be understood as the temperature control of sample liquids over a certain period of time with a predetermined temperature profile.
  • the incubation block has a precisely defined temperature that can be kept constant over a long period of time.
  • the incubation is started by inserting the incubation vessel into the incubation block and ended by removing the incubation vessel from the block.
  • the invention also provides for the temperature of the incubation block to be controlled as a function of time. In this way, a sample liquid can be exposed to changing temperatures during the presence of the incubation vessel in the incubation block. Temperatures that change over time are used, for example, in so-called thermocyclers for amplifying DNA using the "polymerase chain reaction".
  • a system for the incubation of sample liquids in the sense of the invention can represent a so-called "stand-alone module” or a sub-unit within an analysis device.
  • the part of the incubation system that has holes for receiving incubation vessels is referred to as the incubation block.
  • Incubators according to the invention therefore belong to the class of metal block incubators.
  • the incubation block is preferably made from one piece, for example from a cylinder, into which holes are made or by pouring a material into a mold that provides recesses for incubation vessels. Suitable materials for the incubation block are metals, in particular aluminum and also alloys, such as brass.
  • the bores within the incubation block are usually cylindrical in shape or are truncated cones.
  • the term "cylinder” is intended to encompass both cylinders with a round and an angular cross section.
  • the bores are usually a few centimeters deep and preferably taper towards the inside of the incubation block.
  • the incubation block can furthermore have guide elements which facilitate the positioning of a rack according to the invention. Inside the incubation block or on its outside there can be sensors (e.g. light barriers) that detect the presence of a rack.
  • sensors e.g. light barriers
  • the incubation block is still in thermal contact with a temperature control device. If the incubation block is only intended for heating incubation vessels above room temperature, this temperature control device can be a simple electrical heater. However, it is advantageous to provide a possibility for cooling the incubation block in addition to a heating element. Cooling can be achieved, for example, by a part of the incubation block through which cold water flows. For larger incubation systems, a heat pump similar to a refrigerator can also be used for cooling. Devices with which both heating and cooling are possible are Peltier elements that transport heat by means of electrical energy.
  • Said device for tempering the incubation block can either be thermally coupled to the incubation block, as shown, for example, in No. 4,335,620, or the means for tempering can be located within the incubation block itself.
  • the incubation block has a number of bores open at the top, which are usually arranged periodically.
  • these bores are equipped with incubation vessels either manually or with the aid of a robot arm. While manual assembly is time-consuming and personnel-intensive, a device for automatic assembly makes a device relatively complex and therefore expensive. Even with automatic assembly, incubation vessels must be fed to the device from the outside in a form that is suitable for robotics.
  • the incubation block is equipped with the aid of a rack in which incubation vessels are located.
  • the incubation vessels hang in the bores of a holding plate.
  • the bores within the holding plate are arranged corresponding to the bores within the incubation block.
  • the rack is moved over the incubation block and lowered there, so that the incubation vessels move into the holes in the incubation block.
  • a holding plate according to the invention has the shape of a thin disc in which holes are made which serve to hold incubation vessels.
  • the holding plate can be made from a variety of dimensionally stable materials. Metals and especially plastics can be used.
  • the arrangement of the holes in the holding plate corresponds to the arrangement of the holes on the top of the incubation block used in each case.
  • a holding plate can for example be a full circle or preferably a segment of a circle or a section of a circular disk.
  • the thickness of the holding plate must be such that it ensures mechanical stability when incubation vessels are hanging in the holding plate. If the holding plate is made of plastic, then the material thickness usually be one to several millimeters. Of course, a reduction in the material thickness is possible if struts are installed that increase the mechanical stability.
  • the bores within the holding plate can be made with tools in a holding plate made of solid material or they are already provided in the mold for producing the holding plate. According to the invention, the cross section of these bores is of some importance, so that the tolerances for the bore cross section should be less than 0.05 mm.
  • Stand elements can preferably be located on the underside of the holding plate, which ensure that the incubation vessels which hang in the holding plate do not hit the surface.
  • the stand elements make the holding plate a frame in which the incubation vessels can also be stored outside of an incubation device. This is particularly important if the holding plate is to be equipped with incubation vessels at the factory. In this case, the stand elements enable a simpler packaging of the holding plate with the incubation vessels and furthermore easier handling for the user.
  • Tabs can be attached to the top of the holding plate, which a user can grip when he wants to transport the rack.
  • the tabs are particularly helpful when inserting the rack into the incubation block, since gripping the holding plate itself would be uncomfortable here.
  • Incubation vessels are preferably inserted in the bores of the holding plate at the factory. However, the user can also manually equip the holding plate with incubation vessels.
  • Incubation vessels are already widely known in the prior art.
  • Reusable incubation tubes are usually made of glass, while single-use tubes are usually made of plastics. Suitable plastics are, for example, polyethylene, polypropylene. Polystyrene and polymethyl methacrylate.
  • the shape of the incubation tubes usually corresponds to that of tubes with an open and a closed end. As already described above, it is important for the use of incubation vessels in incubation blocks with bores that the outside of the incubation vessels bears against the inner wall of the bores in order to ensure the best possible heat transfer.
  • Tubes which are tapered towards their closed end, ie towards the underside, are preferred for incubation vessels. It has been found that a taper with a slope of 0.05 to 0.5 is particularly favorable for manufacturing reasons.
  • the incubation vessels furthermore have holding devices which prevent the incubation vessels from slipping through the bores in the holding plate.
  • the incubation vessels hang in the holding plate with their closed end pointing downwards. It is possible to hold the incubation vessels if the incubation vessels are tapered and the open end has a cross section which is larger than the cross section of a bore in the holding plate. If such an incubation vessel is introduced into a bore, it slips through until it is at a point where the outer cross section corresponds to the bore diameter. The incubation vessel and holding plate are jammed at this point.
  • the incubation vessel has holding elements which specify a better-defined holding position of the incubation vessel within the holding plate.
  • These holding elements can be webs, for example, which are attached to the outer peripheral surface of the incubation vessel and which cause the effective cross section to suddenly widen at a certain height of the incubation vessel.
  • the incubation vessel is constructed in such a way that a plateau exists which is essentially perpendicular to the longitudinal axis of the incubation vessel and with which the incubation vessels rest on the holding plate.
  • Such a plateau can be generated, for example, by a circular ring which extends around the incubation vessel or the incubation vessel can have a lower part, the cross section of which is smaller than the cross section of the bores and a second part is located on this part, the cross section of which is larger than that Bore cross section.
  • the two parts are preferably connected to one another by a piece of material, preferably perpendicular to the longitudinal axis of the incubation vessel. This connecting piece forms the plateau, which rests on the holding plate.
  • the ratio of the bore cross section and the outer circumference of the incubation vessel, which is located at the level of the holding plate when the incubation vessel is suspended in the holding plate, is important.
  • the incubation vessel must be held by the holding plate with sufficient accuracy within the plane of the holding plate so that the incubation vessels can be introduced into the bores of the incubation block in a targeted manner.
  • the incubation vessel must have some play in the plane of the holding plate within the bore, so that geometrical deviations of the bores in the holding plate and the bores in the incubation block do not lead to jamming of the incubation vessels.
  • a mechanical play between the incubation vessels and the holding plate is of particular importance in the case of incubators, since the temperature differences result in thermal expansions which, in the case of a rigid arrangement of incubation vessels and holding plate, lead to jamming if there is more than one incubation vessel. Even a slight jamming means that a tight fit of the incubation vessels on the inner walls of the bores of the incubation block is no longer guaranteed.
  • a suitable mechanical play can be obtained if the outer cross section of the incubation vessels in the region which is enclosed by the holding plate is 0.2 to 1 mm smaller than the inner cross section of the bores of the holding plate.
  • the part of the incubation vessel which is located below the holding plate is longer than the bores of the incubation block are deep. If the holding plate with the incubation vessels is placed on the incubation block, the incubation vessels hit the bottom of the bores and the devices are lifted off the holding plate. When using conically tapering incubation vessels, this leads to the play between the incubation vessel and the bore in the holding plate being increased, so that jamming can be avoided even better.
  • the holding devices of the incubation vessels stand out from the holding plate. In this way, the incubation vessels are in close contact with the inner walls of the bores even when the incubation vessels exhibit length fluctuations in terms of production technology.
  • the holding plate is supplied equipped with incubation vessels, so that the user only has to insert it into the incubation block.
  • the invention accordingly offers the advantage that the rack according to the invention can be used to easily equip incubators.
  • the advantages of fluid incubators and metal block incubators can accordingly be combined by using a rack according to the invention.
  • a simple assembly of an incubator with a large number of incubation vessels is possible without a large number of manual work steps being necessary, without requiring robotics, or without the disadvantages associated with fluids.
  • the incubation vessel (20) shown is in one piece, but can be divided into two sections.
  • the section (21) tapers downwards and is closed at the bottom.
  • the section (22), which is located above the holding plate (3) when the vessel is suspended in the holding plate, has a cylindrical shape and is open at the top.
  • the transition between the two sections of the incubation vessel is of particular importance. Due to the different outside diameters of the sections mentioned, a plateau (23) is formed between them, with which the incubation vessel rests on the holding plate (3).
  • the plateau (23) has a width of about 0.3 to 0.6 mm.
  • Figure 3 shows a rack (10) in side view with a suspended incubation vessel (20) and free bores (12).
  • the two tabs (11) are located above the holding plate (3). Both the stand elements (4a) and the stand element (4b) can be seen below the holding plate (3).
  • FIG. 4a shows a system for incubation in a perspective view.
  • a rack (10) is placed on the incubation block (1) in such a way that the incubation vessels (20) dip into the bores (2) of the incubation block.
  • the stand elements (4a) slide over the edge of the recess (5) and the stand element (4b) enters the recess (6).
  • a light barrier (24) can also be seen in FIG. 4a, which is located within the incubation block.
  • the light barrier detects the presence of a rack in that a stand element (4b) interrupts the light path at the lower end of the recess (6).
  • a section of this figure is shown in side view in Figure 4b.
  • the incubation vessel (20) is initially suspended in the rack (10). If the incubation vessel is inserted into a hole (2) in the incubation block (1) using the rack, the incubation vessel hits the bottom of the hole in the incubation block.
  • the length of the incubation vessel (20) is dimensioned such that a gap is formed between the plateau (23) and the top of the holding plate (3) when the holding plate lies on the incubation block.
  • FIG. 4b it can also be seen that the space between the edge of the bore and the incubation vessel increases when the incubation vessel is pushed upwards out of the holding plate.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Coating Apparatus (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
EP95117563A 1994-11-11 1995-11-08 Système pour l'incubation des échantillons liquides Expired - Lifetime EP0711603B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4440294 1994-11-11
DE4440294A DE4440294A1 (de) 1994-11-11 1994-11-11 System zur Inkubation von Probeflüssigkeiten

Publications (2)

Publication Number Publication Date
EP0711603A1 true EP0711603A1 (fr) 1996-05-15
EP0711603B1 EP0711603B1 (fr) 2001-08-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP95117563A Expired - Lifetime EP0711603B1 (fr) 1994-11-11 1995-11-08 Système pour l'incubation des échantillons liquides

Country Status (5)

Country Link
US (1) US5670120A (fr)
EP (1) EP0711603B1 (fr)
JP (1) JPH08228756A (fr)
DE (2) DE4440294A1 (fr)
ES (1) ES2162886T3 (fr)

Cited By (5)

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WO1998020975A1 (fr) * 1996-11-08 1998-05-22 Eppendorf-Netheler-Hinz Gmbh Bloc de regulation de temperature dote de dispositifs de regulation de temperature
US6132684A (en) * 1997-10-31 2000-10-17 Becton Dickinson And Company Sample tube holder
DE19926937A1 (de) * 1999-06-14 2001-01-04 Biopsytec Gmbh Vorrichtung zur Aufnahme von Labor Reaktionsgefäßen
DE10243209A1 (de) * 2002-03-22 2003-10-02 Endress & Hauser Wetzer Gmbh Vorrichtung zur Kühlung eines Probennehmers
DE102006062714B4 (de) * 2006-03-09 2013-02-21 Eppendorf Ag Vorrichtung zum Mischen von Laborgefäß-Inhalten

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JP2001514877A (ja) * 1997-08-20 2001-09-18 バイオポア アイエヌシー． 凍結保存を容易にするためのカセット型デバイスおよびシステム
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
EP2156891A3 (fr) 1998-05-01 2010-06-02 Gen-Probe Incorporated Système et méthode pour l'incubation du contenu d'un récipient de réaction
US6117391A (en) * 1998-06-18 2000-09-12 Bayer Corporation Cup handling subsystem for an automated clinical chemistry analyzer system
EP1000661A1 (fr) * 1998-10-29 2000-05-17 Hans-Knöll-Institut für Naturstoff-Forschung e.v. Plaque multi-puits ultramince pour le thermocyclage en utilisant un bloc de chauffage
US6425438B1 (en) 2000-06-13 2002-07-30 Dan-Kar Corporation Method for defrosting a container and a frozen liquid therein
US6825041B2 (en) 2001-03-16 2004-11-30 Beckman Coulter, Inc. Method and system for automated immunochemistry analysis
GB0110449D0 (en) * 2001-04-28 2001-06-20 Genevac Ltd Improvements in and relating to the heating of microtitre well plates in centrifugal evaporators
US7250303B2 (en) * 2001-07-20 2007-07-31 Ortho-Clinical Diagnostics, Inc. Chemistry system for a clinical analyzer
US7459302B2 (en) 2001-10-02 2008-12-02 Stratagene California Side-wall heater for thermocycler device
AU2003206415A1 (en) * 2002-01-07 2003-07-24 Uab Research Foundation Electroporation cuvette-pipette tips, multi-well cuvette arrays, and electrode template apparatus adapted for automation and uses thereof
GB0229410D0 (en) * 2002-12-17 2003-01-22 Molecular Sensing Plc Sample vessel
US8615368B2 (en) 2005-03-10 2013-12-24 Gen-Probe Incorporated Method for determining the amount of an analyte in a sample
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US20110294208A1 (en) * 2009-02-11 2011-12-01 Nancy Allbritton Method and device for cell selection and collection in an isolated culturing environment
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US8592695B2 (en) * 2010-11-30 2013-11-26 Jose Maria Las Navas Garcia Stackable crucible, a system using a stackable crucible, and a method of using a stackable crucible
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CA2908930C (fr) 2012-06-28 2021-08-03 Fluoresentric, Inc. Dispositif indicateur chimique
JP6165961B2 (ja) 2013-03-15 2017-07-19 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ 前処理カルーセルを有する診断分析装置および関連方法
EP4109106A1 (fr) 2013-03-15 2022-12-28 Abbott Laboratories Analyseurs de diagnostic automatisés ayant des corbeilles agencées verticalement et procédés associés
CN107831324B (zh) 2013-03-15 2021-11-19 雅培制药有限公司 具有后面可进入轨道***的自动化诊断分析仪及相关方法
GB2591198B (en) 2014-04-04 2021-10-27 It Is Int Ltd Biochemical reaction system
JP6746574B2 (ja) * 2014-07-28 2020-08-26 ダグラス・サイエンティフィック・エルエルシー 生体試料及び試薬を分析するための器具
US20180264476A1 (en) * 2015-09-16 2018-09-20 Fluoresentric, Inc. Apparatus, systems and methods for dynamic flux amplification of samples
JP7316027B2 (ja) * 2018-05-24 2023-07-27 三菱重工業株式会社 回転軸系の捩れ振動計測装置
CN110205241A (zh) * 2019-04-23 2019-09-06 扬州市海诚生物技术有限公司 一种杀菌效果好且便于夹持试管的生化培养箱

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ES2162886T3 (es) 2002-01-16
EP0711603B1 (fr) 2001-08-16
DE59509510D1 (de) 2001-09-20
US5670120A (en) 1997-09-23
JPH08228756A (ja) 1996-09-10
DE4440294A1 (de) 1996-05-15

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