DK2302049T3 - Cbh2-cellulasevarianter af hypocrea jecorina - Google Patents

Cbh2-cellulasevarianter af hypocrea jecorina Download PDF

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Publication number
DK2302049T3
DK2302049T3 DK10184849.7T DK10184849T DK2302049T3 DK 2302049 T3 DK2302049 T3 DK 2302049T3 DK 10184849 T DK10184849 T DK 10184849T DK 2302049 T3 DK2302049 T3 DK 2302049T3
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Denmark
Prior art keywords
cbh2
variant
cellulase
vol
sequence
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DK10184849.7T
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English (en)
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Wolfgang Aehle
Robert M Caldwell
Lydia Dankmeyer
Frits Goedegebuur
Bradley R Kelemen
Colin Mitchinson
Paulien Neefe
Pauline Teunissen
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Danisco Us Inc
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Claims (16)

  1. CBH2-CELLULASEVARIANTER AF HYPOCREA JECORINA
    1. CBH2-cellulasevarianl afledl af el præcursorprolein ved subslilulion eller delelion ved en position, der svarer til G231 i den modne sekvens af CBH2 fra Hypocrea jecorina, hvor rest G231 i den modne sekvens af CBH2 fra Hypocrea jecorina svarer til rest G255 i figur 1, og hvor varianten har øget termostabilitet sammenlignet med præcursorproteinet.
  2. 2. CBH2-cellulasevariant ifølge krav 1, hvor varianten omfatter en substitution ved en position, der svarer til G231N i den modne sekvens af CBH2 fra Hypocrea jecorina.
  3. 3. CBH2-cellulasevariant ifølge krav 1 eller 2, hvor varianten endvidere omfatter en substitution eller deletion ved en position, der svarer til én eller flere af resterne V94, P98, GI 18, M120, M134, T142, L144, Ml45, T148, TI54, E179, Q204, V206, S210, 1212, T214, E215, T232, V250, Q276, N285, S291, G308, T312, S316, V323, N325, 1333, G334, S343, T349, G360, S380, A381, S386, F411, S413, A416, Q426 og/eller A429 i den modne sekvens af CBH2 fra Hypocrea jecorina.
  4. 4. CBH2-cellulasevariant ifølge krav 3, hvor varianten omfatter en substitution ved en position, der svarer til én eller flere af resterne V94E, P98L, G118P, M120L, M134(G/L/V), T142V, L144(G/R/S), M145L, T148Y, T154A, L179A, Q204E, V206L, S210(L/R), I212V, T214(M/Y), L215I, T232V, V250I, Q276L, N285Q, S291G, G308A, T312S, S316P, V323(L/N/Y), N325D, I333L, G334A, S343P, T349L, G360R, S380T, A381T, S386P, F411Y, S413Y, A416G, Q426E og/eller A429T i den modne sekvens af CBH2 fra Hypocrea jecorina.
  5. 5. Nukleinsyre, der koder for en CHB2-variant ifølge et hvilket som helst af kravene 1 til 4.
  6. 6. Vektor, der omfatter en nukleinsyre, der koder for en CBH2-variant ifølge krav 5.
  7. 7. Værtscelle transformeret med vektoren ifølge krav 6,
  8. 8. Fremgangsmåde til fremstilling af en CBH2-variant, hvilken fremgangsmåde omfatter følgende trin: (a) dyrkning af værtscellen ifølge krav 7 i et egnet dyrkningsmedium under egnede forhold til at fremstille CBH2-variant; (b) opnåelse af den fremstillede CBH2-variant.
  9. 9. Vaskemiddelsammensætning omfattende et overfladeaktivt middel og en CBH2-variant, hvor CBH2-varianten omfatter en CBH2-variant ifølge et hvilket som helst af kravene 1 til 4.
  10. 10. Vaskemiddel ifølge krav 9, hvor vaskemidlet er et tøjvaskemiddel eller et opvaskemiddel.
  11. 11. Fodertilsætningsstof omfattende en CBH2-variant ifølge et hvilket som helst af kravene 1 til 4.
  12. 12. Fremgangsmåde til behandling af træmasse omfattende etablering af kontakt mellem træmassen og en CBH2-variant ifølge et hvilket som helst af kravene 1 til 4.
  13. 13. Fremgangsmåde til omdannelse af biomasse til sukkere, hvilken fremgangsmåde omfatter etablering af kontakt mellem biomassen og en CBH2-variant ifølge et hvilket som helst af kravene 1 til 4.
  14. 14. Fremgangsmåde til behandling af et celluloseholdigt tekstil, hvilken fremgangsmåde omfatter etablering af kontakt mellem det celluloseholdige tekstil og en CBH2-variant ifølge et hvilket som helst af kravene 1 til 4.
  15. 15. Fremgangsmåde ifølge krav 14, hvor det celluloseholdige tekstil omfatter indigofarvet denim.
  16. 16. Fremgangsmåde til fremstilling af en CBH2-cellulasevariant, hvor varianten omfatter en substitution eller deletion ved en position svarende til G231 i den modne sekvens af CBH2 fra Hypocrea jecorina, hvor rest G231 i den modne sekvens af CBH2 fra Hypocrea jecorina svarer til rest G255 i figur 1, hvilken fremgangsmåde omfatter: (i) tilvejebringelse af en præcursor CBH2-cellulase; (ii) substitution eller deletering af aminosyreresten ved positionen i præcursor CBH2-cellulasen G231 i den modne sekvens af CBH2 fra Hypocrea jecorina; hvor varianten har øget termostabilitet sammenlignet med præeursorproteinet.
DK10184849.7T 2004-12-30 2005-12-22 Cbh2-cellulasevarianter af hypocrea jecorina DK2302049T3 (da)

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Application Number Priority Date Filing Date Title
US64039804P 2004-12-30 2004-12-30
US65686305P 2005-02-25 2005-02-25
US66607205P 2005-03-28 2005-03-28
EP05855774.5A EP1838849B1 (en) 2004-12-30 2005-12-22 Variant hypocrea jecorina cbh2 cellulases

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EP (4) EP2302049B1 (da)
JP (3) JP5148288B2 (da)
CN (2) CN102634499B (da)
BR (1) BRPI0519456A2 (da)
CA (1) CA2592550C (da)
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ES (1) ES2554467T3 (da)
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