DK2496693T3 - Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem - Google Patents

Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem Download PDF

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DK2496693T3
DK2496693T3 DK10776908.5T DK10776908T DK2496693T3 DK 2496693 T3 DK2496693 T3 DK 2496693T3 DK 10776908 T DK10776908 T DK 10776908T DK 2496693 T3 DK2496693 T3 DK 2496693T3
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polypeptide
gly
ala
thr
ser
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DK10776908.5T
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Elena Vlasenko
Mary Stringer
Kirk Schnorr
Brett Mcbrayer
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Novozymes Inc
Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (12)

1. Isoleret polypeptid med cellobiohydrolaseaktivitet, som er valgt fra gruppen beståend af: (a) et polypeptid, som omfatter en aminosyresekvens, der udviser mindst 80‘ identitet med aminosyrerne 17-447 ifølge SEQ ID NO: 2; (b) et polypeptid, som kodes for af et polynukleotid, der omfatter e nukleotidsekvens, som udviser mindst 80% identitet med nukleotiderne 109-1401 ifølge SEQ I NO: 1; og (c) et fragment af et polypeptid ifølge (a) eller (b), som h; cellobiohydrolaseaktivitet, hvilket fragment indeholder mindst 390 aminosyrerester.
2. Polypeptid ifølge krav 1, som omfatter eller består af aminosyrerne 17 til 447 ifølge SE' ID NO: 2; eller et fragment deraf, der indeholder mindst 390 aminosyrerester og h; cellobiohydrolaseaktivitet.
3. Polypeptid ifølge krav 1, hvor fragmentet af et polypeptid ifølge (a) eller (b), som h; cellobiohydrolaseaktivitet, indeholder mindst 430 aminosyrerester.
4. Isoleret polynukleotid, som omfatter en nukleotidsekvens, der koder for polypeptidi ifølge et hvilket som helst af kravene 1-3.
5. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-: hvilken fremgangsmåde omfatter: (a) dyrkning af en værtscelle, som omfatter e nukleinsyrekonstruktion, hvilken nukleinsyrekonstruktion omfatter en nukleotidsekvens, der kod< for polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (I indvinding af polypeptidet.
6. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt material· hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med e enzymsammensætning i nærværelse af polypeptidet ifølge et hvilket som helst af kravene 1-3.
7. Fremgangsmåde ifølge krav 6, som endvidere omfatter indvinding af det nedbrudl celluloseholdige materiale.
8. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåd omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning nærværelse af polypeptidet ifølge et hvilket som helst af kravene 1-3; (b) fermentering af det forsukrede celluloseholdige materiale med en eller fler (adskillige) fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
9. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilke fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller fler (adskillige) fermenterende mikroorganismer, hvor det celluloseholdige materiale erforsukret me en enzymsammensætning i nærværelse af polypeptidet ifølge et hvilket som helst af kravene 1 3.
10. Fremgangsmåde ifølge krav 9, hvor fermenteringen af det celluloseholdige material frembringer et fermenteringsprodukt.
11. Fremgangsmåde ifølge krav 10, som endvidere omfatter indvinding i fermenteringsproduktet fra fermenteringen.
12. Fremgangsmåde ifølge krav 10 eller 11, hvor fermenteringsproduktet er en alkohol, e organisk syre, en keton, en aminosyre eller en gas.
DK10776908.5T 2009-11-06 2010-11-05 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem DK2496693T3 (da)

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US25899909P 2009-11-06 2009-11-06
US31043010P 2010-03-04 2010-03-04
PCT/US2010/055643 WO2011057086A1 (en) 2009-11-06 2010-11-05 Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

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DK2496693T3 true DK2496693T3 (da) 2018-01-22

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US (2) US9267125B2 (da)
EP (1) EP2496693B1 (da)
CN (1) CN103068976A (da)
BR (1) BR112012008260A2 (da)
CA (1) CA2780176A1 (da)
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WO (1) WO2011057086A1 (da)

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US20120272410A1 (en) 2012-10-25
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US20160160201A1 (en) 2016-06-09
CA2780176A1 (en) 2011-05-12
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BR112012008260A2 (pt) 2015-09-15
EP2496693B1 (en) 2017-10-25

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