DK2576796T3 - Filamentøse svampeværtsstammer og dna-konstrukter samt fremgangsmåder til anvendelse deraf - Google Patents
Filamentøse svampeværtsstammer og dna-konstrukter samt fremgangsmåder til anvendelse deraf Download PDFInfo
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- DK2576796T3 DK2576796T3 DK11727041.3T DK11727041T DK2576796T3 DK 2576796 T3 DK2576796 T3 DK 2576796T3 DK 11727041 T DK11727041 T DK 11727041T DK 2576796 T3 DK2576796 T3 DK 2576796T3
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- host cell
- filamentous fungal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (12)
- FILAMENTØSE SVAMPEVÆRTSSTAMMER OG DNA-KONSTRUKTER SAMT FREMGANGSMÅDER TIL ANVENDELSE DERAF1. Eilamentøs svampe værtscelle-ekspressionssystem, der omfatter: a. en svampeværtscelle, der i sit kromosomale DNA indeholder en disruption i én eller flere komponenter af den ikke-homologe rekombinations- (NHR) bane, en del af en første valgbar markør, der mangler en første valgbar funktion, og en anden valgbar markør, der kan anvendes til at tildele en anden valgbar funktion; og b. et nukleinsyremolekyle indeholdende en sekvens, der, når den introduceres i svampeværtscellen, tildeler den første valgbare markør den første valgbare funktion, en sekvens, der kan anvendes til at udtrykke ét eller flere gener af interesse, og sekvenser med væsentlig homologi med sekvenser, der flankerer de kromosomale valgbare markører; hvor de homologe sekvenser bevirker et homologt rekombinations-event, der resulterer i en første funktionel valgbar markør, fjernelse af den anden valgbare markør og ekspression af genet af interesse, og hvor den første valgbare markør og den anden valgbare markør er forskellige markører.
- 2. Filamentøs svampeværtscelle-ekspressionssystem ifølge krav 1, hvor den ene eller flere komponenter af NHR-banen er udvalgt fra gruppen bestående af ku80, ku70, rad50, mrell, xrs2, lig4 og xrs.
- 3. Filamentøs svampeværtscelle-ekspressionssystem ifølge krav 1, hvor den første valgbare markør og den anden valgbare markør er forskellige markører udvalgt fra gruppen bestående af alsR, amdS, hygR, pyr2, pyr4, pyrG, sucA, en bleomycinresistensmarkør, en blasticidinresistensmarkør, en pyrithiaminresistensmarkør, en chlorimuronethylresistensmarkør, en neomycinresistensmarkør, et adeninbanegen, et tryptophanbanegen og thymidinkinase.
- 4. Filamentøs svampeværtscelle-ekspressionssystem ifølge krav 1, hvor svampeværtscellen er fra en art, der er udvalgt fra gruppen bestående af Trichoderma, Penicillium, Aspergillus, Humicola, Chrysosporium, Fusarium, Neurospora og Emericella.
- 5. Filamentøs svampeværtscelle-ekspressionssystem ifølge krav 4, hvor Trichoderma er T. reesei.
- 6. Filamentøs svampe værtscelle-ekspressionssystem ifølge krav 4, hvor Aspergillus er A. niger.
- 7. Filamentøs svampeværtscelle-ekspressionssystem ifølge krav 1, hvor genet af interesse er udvalgt fra gruppen bestående af hemicellulaser, peroxidaser, proteaser, cellulaser, xylanaser, lipaser, phospholipaser, esteraser, cutinaser, pectinaser, keratinaser, reduktaser, oxidaser, phenoloxidaser, lipoxygenaser, ligninaser, pullulanaser, tannaser, pentosanaser, malanaser, beta-glucanaser, arabinosidaser, hyaluronidase, chondroitinase, laccase, amylaser, glucoamylaser og blandinger deraf.
- 8. Filamentøs svampeværtscelle-ekspressionssystem ifølge krav 1, hvor genet af interesse er udvalgt fra gruppen bestående af acetylesteraser, aminopeptidaser, amylaser, arabinaser, arabinofuranosidaser, carboxypeptidaser, catalaser, cellulaser, chitinaser, chymosin, cutinase, deoxyribonucleaser, epimeraser, esteraser, a-galactosidaser, β-galac tos idaser, a-glucanaser, glucanlysaser, endo^-glucanaser, glucoamylaser, glucoseoxidaser, a-glucosidaser, β-glucosidaser, glucuronidaser, hemicellulaser, hexoseoxidaser, hydrolaser, invertaser, isomeraser, laccaser, lipaser, lyaser, mannosidaser, oxidaser, oxidoreduktaser, pectatlyaser, pectinacetylresteraser, pectinrdepolymeraser, pectinmethylesteraser, pectinolytiske enzymer, peroxidaser, phenoloxidaser, phytaser, polygalacturonaser, proteaser, rhamno-galacturonaser, ribonucleaser, thaumatin, transferaser, transportproteiner, transglutaminaser, xylanaser, hexoseoxidaser og kombinationer deraf.
- 9. Filamentøs svampeværtscelle-ekspressionssystem ifølge krav 1, hvor genet af interesse er udvalgt fra gruppen bestående af peptidhormoner, vækstfaktorer, koaguleringsfaktorer, chemokiner, cytokiner, lymfokiner, antistoffer, receptorer, adhæsionsmolekyler, mikrobielle antigener og fragmenter deraf.
- 10. Fremgangsmåde til ekspression af et gen af interesse i det filamentøse svampeværtscellesystem ifølge et hvilket som helst af kravene 1 til 9, hvilken fremgangsmåde omfatter introduktion i den filamentøse svampeværtscelle af nukleinsyremolekylet, dyrkning af værtscellerne og udvælgelse af de værtsceller, der har den første valgbare funktion, men mangler den anden valgbare funktion.
- 11. Fremgangsmåde ifølge krav 10, hvilken fremgangsmåde endvidere omfatter udførelse af assay for ekspression af genet af interesse.
- 12. Fremgangsmåde ifølge krav 11, hvilken fremgangsmåde endvidere omfatter udførelse af assay for en biokemisk funktion af ct polypeptid, der cr kodet for af genet af interesse.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35128610P | 2010-06-03 | 2010-06-03 | |
| PCT/US2011/039092 WO2011153449A1 (en) | 2010-06-03 | 2011-06-03 | Filamentous fungal host strains and dna constructs, and methods of use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2576796T3 true DK2576796T3 (da) | 2017-06-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11727041.3T DK2576796T3 (da) | 2010-06-03 | 2011-06-03 | Filamentøse svampeværtsstammer og dna-konstrukter samt fremgangsmåder til anvendelse deraf |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9701969B2 (da) |
| EP (1) | EP2576796B1 (da) |
| JP (1) | JP2013533737A (da) |
| CN (1) | CN102939382B (da) |
| BR (1) | BR112012030746A2 (da) |
| CA (1) | CA2801799C (da) |
| DK (1) | DK2576796T3 (da) |
| WO (1) | WO2011153449A1 (da) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103184211B (zh) * | 2011-12-27 | 2014-09-24 | 东北农业大学 | 一种与耐氯嘧磺隆相关蛋白及其编码基因与应用 |
| CN105992821A (zh) * | 2013-12-03 | 2016-10-05 | 诺维信公司 | 真菌基因文库的双***标记物整合 |
| JP2017525359A (ja) * | 2014-08-15 | 2017-09-07 | ダニスコ・ユーエス・インク | タンパク質の産生改善のための組成物及び方法 |
| CN106661573B (zh) * | 2014-08-20 | 2021-07-27 | 诺维信公司 | 多核苷酸文库的重组酶介导的整合 |
| EP3227430B1 (en) * | 2014-12-01 | 2021-05-19 | Danisco US Inc. | Fungal host strains, dna constructs, and methods of use |
| BR112017012837A2 (pt) * | 2014-12-16 | 2017-12-26 | Danisco Us Inc | sistemas de modificação de genoma fúngico e métodos de uso |
| CN108064267A (zh) * | 2015-02-09 | 2018-05-22 | 丹尼斯科美国公司 | 真菌菌株及使用方法 |
| CN104894115B (zh) * | 2015-05-26 | 2018-11-13 | 中国科学院青岛生物能源与过程研究所 | 一种具有高效同源重组能力的土曲霉及其构建方法与应用 |
| CN104894165B (zh) * | 2015-05-26 | 2019-01-25 | 中国科学院青岛生物能源与过程研究所 | 一种提高基因打靶技术在土曲霉中应用效率的方法与应用 |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| BR112018011503A2 (pt) | 2015-12-07 | 2018-12-04 | Zymergen Inc | promotores da corynebacterium glutamicum |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| EP3390625B1 (en) | 2015-12-18 | 2023-09-06 | Danisco US Inc. | Polypeptides with endoglucanase activity and uses thereof |
| EP3239898A1 (en) * | 2016-04-27 | 2017-11-01 | Mercarista, S.L. | Method for quantifying proteins in a sample |
| EP3478845A4 (en) | 2016-06-30 | 2019-07-31 | Zymergen, Inc. | METHODS OF PRODUCING A GLUCOSE PERMEASE BANK AND USES THEREOF |
| WO2018005655A2 (en) | 2016-06-30 | 2018-01-04 | Zymergen Inc. | Methods for generating a bacterial hemoglobin library and uses thereof |
| WO2018067599A1 (en) | 2016-10-04 | 2018-04-12 | Danisco Us Inc. | Protein production in filamentous fungal cells in the absence of inducing substrates |
| CA3048658A1 (en) * | 2016-12-30 | 2018-07-05 | Zymergen Inc. | A method to build fungal production strains using automated steps for genetic manipulation and strain purification |
| CN108588060B (zh) | 2017-03-07 | 2020-12-01 | 武汉康复得生物科技股份有限公司 | 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶 |
| EP3635112A2 (en) | 2017-06-06 | 2020-04-15 | Zymergen, Inc. | A htp genomic engineering platform for improving fungal strains |
| CN108004153B (zh) * | 2017-12-07 | 2021-05-28 | 潍坊康地恩生物科技有限公司 | 一株高产果胶裂解酶的里氏木霉菌株及其应用 |
| KR20210018219A (ko) | 2018-06-06 | 2021-02-17 | 지머젠 인코포레이티드 | 발효 및 생산 중 곰팡이 형태를 제어하기 위한 신호 전달에 포함된 유전자의 조작 |
| EP4055177B1 (en) | 2019-11-08 | 2024-07-31 | Danisco US Inc. | Fungal strains comprising enhanced protein productivity phenotypes and methods thereof |
| WO2021216302A1 (en) | 2020-04-22 | 2021-10-28 | Danisco Us Inc | Compositions and methods for enhanced protein production in filamentous fungal cells |
| US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
| CN117716039A (zh) | 2021-07-19 | 2024-03-15 | 丹尼斯科美国公司 | 用于增强真菌细胞中的蛋白质产生的组合物和方法 |
| CN114717268A (zh) * | 2021-11-01 | 2022-07-08 | 天津农学院 | 一种可用于无痕遗传转化菌株的制备 |
| WO2023102315A1 (en) | 2021-12-01 | 2023-06-08 | Danisco Us Inc. | Compositions and methods for enhanced protein production in fungal cells |
| WO2024102556A1 (en) | 2022-11-11 | 2024-05-16 | Danisco Us Inc. | Filamentous fungal strains comprising enhanced protein productivity phenotypes and methods thereof |
| WO2024137350A2 (en) | 2022-12-19 | 2024-06-27 | Danisco Us Inc. | Recombinant fungal strains and methods thereof for producing consistent proteins |
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| US5475101A (en) | 1990-10-05 | 1995-12-12 | Genencor International, Inc. | DNA sequence encoding endoglucanase III cellulase |
| US5246853A (en) | 1990-10-05 | 1993-09-21 | Genencor International, Inc. | Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I |
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| FI932521A0 (fi) | 1993-06-02 | 1993-06-02 | Alko Ab Oy | Nytt endoglukanasenzym |
| DE69822142T2 (de) | 1997-04-07 | 2005-03-03 | Unilever N.V. | Transformation von schimmeln durch agrobacterium, insbesondere von der gattung aspergillus |
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| DE602007012846D1 (de) * | 2006-04-08 | 2011-04-14 | Dsm Ip Assets Bv | Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen |
| DK2069512T3 (da) | 2006-09-22 | 2010-09-20 | Danisco Us Inc Genencor Div | Acetolaktat-syntase fra Trichoderma reesei som selektionsmarkør |
| US20100120154A1 (en) * | 2007-03-21 | 2010-05-13 | Dsm Ip Assets B.V. | Method for homologous recombination |
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-
2011
- 2011-06-03 BR BR112012030746A patent/BR112012030746A2/pt not_active Application Discontinuation
- 2011-06-03 JP JP2013513379A patent/JP2013533737A/ja active Pending
- 2011-06-03 CN CN201180026764.2A patent/CN102939382B/zh active Active
- 2011-06-03 US US13/701,479 patent/US9701969B2/en active Active
- 2011-06-03 WO PCT/US2011/039092 patent/WO2011153449A1/en active Application Filing
- 2011-06-03 EP EP11727041.3A patent/EP2576796B1/en active Active
- 2011-06-03 DK DK11727041.3T patent/DK2576796T3/da active
- 2011-06-03 CA CA2801799A patent/CA2801799C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2576796B1 (en) | 2017-03-29 |
| CN102939382B (zh) | 2017-02-15 |
| EP2576796A1 (en) | 2013-04-10 |
| CA2801799A1 (en) | 2011-12-08 |
| CA2801799C (en) | 2018-11-20 |
| CN102939382A (zh) | 2013-02-20 |
| JP2013533737A (ja) | 2013-08-29 |
| US20130149742A1 (en) | 2013-06-13 |
| BR112012030746A2 (pt) | 2016-02-16 |
| US9701969B2 (en) | 2017-07-11 |
| WO2011153449A1 (en) | 2011-12-08 |
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