DE69032483T2 - Zellfreie synthese und isolierung von genen und polypeptiden - Google Patents

Zellfreie synthese und isolierung von genen und polypeptiden

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Publication number
DE69032483T2
DE69032483T2 DE69032483T DE69032483T DE69032483T2 DE 69032483 T2 DE69032483 T2 DE 69032483T2 DE 69032483 T DE69032483 T DE 69032483T DE 69032483 T DE69032483 T DE 69032483T DE 69032483 T2 DE69032483 T2 DE 69032483T2
Authority
DE
Germany
Prior art keywords
polypeptides
isolation
cell
genes
free synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69032483T
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English (en)
Other versions
DE69032483D1 (de
Inventor
Glenn Kawasaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Optein Inc
Original Assignee
Optein Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Optein Inc filed Critical Optein Inc
Publication of DE69032483D1 publication Critical patent/DE69032483D1/de
Application granted granted Critical
Publication of DE69032483T2 publication Critical patent/DE69032483T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/02Acyclic radicals, not substituted by cyclic structures
    • C07H15/12Acyclic radicals, not substituted by cyclic structures attached to a nitrogen atom of the saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1041Ribosome/Polysome display, e.g. SPERT, ARM
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6867Replicase-based amplification, e.g. using Q-beta replicase
DE69032483T 1989-10-05 1990-10-04 Zellfreie synthese und isolierung von genen und polypeptiden Expired - Lifetime DE69032483T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41735789A 1989-10-05 1989-10-05
PCT/US1990/005682 WO1991005058A1 (en) 1989-10-05 1990-10-04 Cell-free synthesis and isolation of novel genes and polypeptides

Publications (2)

Publication Number Publication Date
DE69032483D1 DE69032483D1 (de) 1998-08-20
DE69032483T2 true DE69032483T2 (de) 1998-11-26

Family

ID=23653670

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69032483T Expired - Lifetime DE69032483T2 (de) 1989-10-05 1990-10-04 Zellfreie synthese und isolierung von genen und polypeptiden

Country Status (11)

Country Link
US (2) US5658754A (de)
EP (1) EP0494955B1 (de)
JP (2) JP3127158B2 (de)
KR (3) KR0185192B1 (de)
AT (1) ATE168416T1 (de)
AU (1) AU638762B2 (de)
CA (1) CA2067194C (de)
DE (1) DE69032483T2 (de)
DK (1) DK0494955T3 (de)
ES (1) ES2118066T3 (de)
WO (1) WO1991005058A1 (de)

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EP0494955A1 (de) 1992-07-22
CA2067194C (en) 2003-03-18
ES2118066T3 (es) 1998-09-16
WO1991005058A1 (en) 1991-04-18
KR0185192B1 (ko) 1999-04-01
US5658754A (en) 1997-08-19
EP0494955B1 (de) 1998-07-15
DK0494955T3 (da) 1998-10-26
EP0494955A4 (en) 1992-08-12
AU638762B2 (en) 1993-07-08
KR100204359B1 (en) 1999-06-15
KR100204360B1 (en) 1999-06-15
JP3127158B2 (ja) 2001-01-22
JPH05503000A (ja) 1993-05-27
AU6537390A (en) 1991-04-28
DE69032483D1 (de) 1998-08-20
CA2067194A1 (en) 1991-04-06
ATE168416T1 (de) 1998-08-15
KR920703839A (ko) 1992-12-18
US5643768A (en) 1997-07-01

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