DE602004026343D1 - Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen - Google Patents

Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen

Info

Publication number
DE602004026343D1
DE602004026343D1 DE602004026343T DE602004026343T DE602004026343D1 DE 602004026343 D1 DE602004026343 D1 DE 602004026343D1 DE 602004026343 T DE602004026343 T DE 602004026343T DE 602004026343 T DE602004026343 T DE 602004026343T DE 602004026343 D1 DE602004026343 D1 DE 602004026343D1
Authority
DE
Germany
Prior art keywords
functional group
branched alkyl
target protein
chromatography
hydrophobic interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004026343T
Other languages
English (en)
Inventor
Abhinav A Shukla
Sanchayita Ghose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34549339&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602004026343(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of DE602004026343D1 publication Critical patent/DE602004026343D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE602004026343T 2003-10-24 2004-10-22 Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen Active DE602004026343D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51448603P 2003-10-24 2003-10-24
PCT/US2004/035007 WO2005042569A1 (en) 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Publications (1)

Publication Number Publication Date
DE602004026343D1 true DE602004026343D1 (de) 2010-05-12

Family

ID=34549339

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004026343T Active DE602004026343D1 (de) 2003-10-24 2004-10-22 Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen

Country Status (8)

Country Link
US (1) US7427659B2 (de)
EP (1) EP1687328B1 (de)
AT (1) ATE462716T1 (de)
AU (1) AU2004285928B2 (de)
CA (1) CA2542951C (de)
DE (1) DE602004026343D1 (de)
ES (1) ES2342539T3 (de)
WO (1) WO2005042569A1 (de)

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US20070015133A1 (en) * 2005-07-13 2007-01-18 Umc Utrecht Holding B.V. Method for detecting and/or removing protein and/or peptide comprising a cross-beta structure from an aqueous solution comprising a protein
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US8188242B2 (en) * 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
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MX2011013417A (es) 2009-06-25 2012-03-29 Amgen Inc Procesos de purificacion por captura para proteinas expresadas en un sistema no mamifero.
AU2010313395A1 (en) 2009-10-30 2012-05-10 Abbvie Inc. sORF constructs and multiple gene expression
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EP2702077A2 (de) 2011-04-27 2014-03-05 AbbVie Inc. Verfahren zur steuerung des galactosylierungsprofil von rekombinant exprimierten proteinen
EP2726496B1 (de) 2011-07-01 2018-04-04 F.Hoffmann-La Roche Ag Verfahren zum trennen von monomeren polypeptiden von aggregierten polypeptiden
WO2013009526A1 (en) 2011-07-08 2013-01-17 Merck Sharp & Dohme Corp. Method for purifying fc-fusion protein
KR101454316B1 (ko) * 2011-08-17 2014-10-27 한화케미칼 주식회사 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법
SG10201701224UA (en) 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013176754A1 (en) * 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
AU2013309506A1 (en) 2012-09-02 2015-03-12 Abbvie Inc. Methods to control protein heterogeneity
AU2013381687A1 (en) 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
PL2970378T3 (pl) * 2013-03-15 2021-12-06 Biogen Ma Inc. Chromatografia oddziaływań hydrofobowych białek w warunkach bez soli
EP3052640A2 (de) 2013-10-04 2016-08-10 AbbVie Inc. Verwendung von metallionen zur modulation von proteinglykosylierungsprofilen rekombinanter proteine
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101975771B1 (ko) 2013-12-12 2019-05-09 이엠디 밀리포어 코포레이션 아크릴아미드 함유 필터를 사용한 단백질 분리
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TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
MY196321A (en) 2016-08-16 2023-03-24 Regeneron Pharma Methods for Quantitating Individual Antibodies From a Mixture
WO2018081203A1 (en) 2016-10-25 2018-05-03 Regeneron Pharmaceuticals, Inc. Methods and systems for chromatography data analysis
CA3067735A1 (en) 2017-08-17 2019-02-21 Just Biotherapeutics, Inc. Method of purifying glycosylated protein from host cell galectins and other contaminants
TW202005694A (zh) 2018-07-02 2020-02-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
KR20230124578A (ko) 2020-12-22 2023-08-25 암젠 인크 세포 배양 방법
US20240199688A1 (en) 2022-05-02 2024-06-20 Regeneron Pharmaceuticals, Inc. Methods for reducing lipase activity

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WO2005042569A1 (en) * 2003-10-24 2005-05-12 Amgen, Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Also Published As

Publication number Publication date
EP1687328A1 (de) 2006-08-09
ATE462716T1 (de) 2010-04-15
EP1687328A4 (de) 2006-11-15
CA2542951C (en) 2016-08-30
ES2342539T3 (es) 2010-07-08
WO2005042569A1 (en) 2005-05-12
EP1687328B1 (de) 2010-03-31
US7427659B2 (en) 2008-09-23
CA2542951A1 (en) 2005-05-12
US20050136521A1 (en) 2005-06-23
AU2004285928B2 (en) 2012-02-02
AU2004285928A1 (en) 2005-05-12

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