MX2010002818A - Procedimientos de separacion de proteinas recombinantes en sistemas acuosos de dos fases. - Google Patents
Procedimientos de separacion de proteinas recombinantes en sistemas acuosos de dos fases.Info
- Publication number
- MX2010002818A MX2010002818A MX2010002818A MX2010002818A MX2010002818A MX 2010002818 A MX2010002818 A MX 2010002818A MX 2010002818 A MX2010002818 A MX 2010002818A MX 2010002818 A MX2010002818 A MX 2010002818A MX 2010002818 A MX2010002818 A MX 2010002818A
- Authority
- MX
- Mexico
- Prior art keywords
- proteins
- recombinant proteins
- choline
- affinity
- separating
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 abstract 3
- 229960001231 choline Drugs 0.000 abstract 3
- 239000007864 aqueous solution Substances 0.000 abstract 2
- 230000001413 cellular effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
El objeto de la presente invención es un procedimiento para el reparto, separación y purificación en solución acuosa de proteínas recombinantes, basado en la utilización de polipéptidos con afinidad por colina. La invención se fundamenta en un fenómeno consistente en que dos soluciones acuosas con determinados componentes pueden ser mezcladas repartiéndose finalmente en dos fases con distinta densidad. Las proteínas fusionadas a dichos polipéptidos con afinidad por colina se localizan preferentemente en una de las fases, mientras que la mayoría de las proteínas de un extracto celular tienden a irse a la fase contraria. Tras una serie de lavados para eliminar el resto de material no deseado, esta localización puede invertirse mediante la adición de una molécula soluble con afinidad por el polipéptido fusionado a la proteína de interés. Este procedimiento permite modular a conveniencia la presencia de la proteína o polipéptido de interés en una fase u otra, posibilitando su purificación con un alto rendimiento y grado de pureza. La invención representa una forma económica y escalable de separación de proteínas recombinantes etiquetadas preferentemente con dominios de unión a colina.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200702504A ES2319844B1 (es) | 2007-09-12 | 2007-09-12 | Procedimiento de separacion de proteinas recombinantes en sistemas acuosos de dos fases. |
| PCT/ES2008/000578 WO2009034203A1 (es) | 2007-09-12 | 2008-09-05 | Procedimientos de separación de proteínas recombinantes en sistemas acuosos de dos fases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2010002818A true MX2010002818A (es) | 2010-09-09 |
Family
ID=40451605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2010002818A MX2010002818A (es) | 2007-09-12 | 2008-09-05 | Procedimientos de separacion de proteinas recombinantes en sistemas acuosos de dos fases. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8329877B2 (es) |
| EP (1) | EP2196470B1 (es) |
| JP (1) | JP5271354B2 (es) |
| CN (1) | CN101835790A (es) |
| CA (1) | CA2699529A1 (es) |
| ES (1) | ES2319844B1 (es) |
| MX (1) | MX2010002818A (es) |
| WO (1) | WO2009034203A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014029894A1 (es) * | 2012-08-20 | 2014-02-27 | Biomedal, S.L. | Método de separación de polipéptidos etiquetados |
| FI127353B (en) | 2013-07-05 | 2018-04-13 | Teknologian Tutkimuskeskus Vtt Oy | Concentration and purification of hydrophobins and antibodies by a phase separation method |
| CN114632349A (zh) * | 2017-06-01 | 2022-06-17 | 相达生物科技美国有限公司 | 用于多孔材料中双水相分离的相分离行为改性剂 |
| US12043826B2 (en) | 2018-01-19 | 2024-07-23 | Phase Scientific International, Ltd. | Spontaneous nucleic acid purification and concentration in a single step |
| CN108164586B (zh) * | 2018-01-22 | 2021-03-26 | 西南医科大学 | 合成多肽及其应用 |
| CN111856024B (zh) * | 2019-04-28 | 2022-01-28 | 清华大学 | 检测生物膜蛋白质间相互作用的方法及所用成套试剂 |
| CN111876451B (zh) * | 2020-08-10 | 2022-05-20 | 武汉轻工大学 | 一种从鸡粪中提取氨基酸的方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3329508A (en) * | 1965-03-05 | 1967-07-04 | Us Vitamin Pharm Corp | Artificial sweetening compositions containing choline |
| CN1249079C (zh) * | 1999-03-25 | 2006-04-05 | 瓦申泰克尼利南塔基马斯凯斯库斯公司 | 蛋白质的分配方法 |
-
2007
- 2007-09-12 ES ES200702504A patent/ES2319844B1/es not_active Expired - Fee Related
-
2008
- 2008-09-05 JP JP2010524533A patent/JP5271354B2/ja not_active Expired - Fee Related
- 2008-09-05 CA CA2699529A patent/CA2699529A1/en not_active Abandoned
- 2008-09-05 WO PCT/ES2008/000578 patent/WO2009034203A1/es active Application Filing
- 2008-09-05 CN CN200880112626A patent/CN101835790A/zh active Pending
- 2008-09-05 MX MX2010002818A patent/MX2010002818A/es not_active Application Discontinuation
- 2008-09-05 EP EP08830569.3A patent/EP2196470B1/en not_active Not-in-force
-
2010
- 2010-03-12 US US12/723,356 patent/US8329877B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2319844B1 (es) | 2010-02-16 |
| CN101835790A (zh) | 2010-09-15 |
| JP2010539142A (ja) | 2010-12-16 |
| EP2196470A1 (en) | 2010-06-16 |
| ES2319844A1 (es) | 2009-05-12 |
| EP2196470B1 (en) | 2013-06-05 |
| US20100234567A1 (en) | 2010-09-16 |
| CA2699529A1 (en) | 2009-03-19 |
| WO2009034203A1 (es) | 2009-03-19 |
| EP2196470A4 (en) | 2011-08-17 |
| US8329877B2 (en) | 2012-12-11 |
| JP5271354B2 (ja) | 2013-08-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |