CY1110092T1 - ΠΑΡΑΣΚΕΥΗ TNFR-Fc - Google Patents

ΠΑΡΑΣΚΕΥΗ TNFR-Fc

Info

Publication number
CY1110092T1
CY1110092T1 CY20101100556T CY101100556T CY1110092T1 CY 1110092 T1 CY1110092 T1 CY 1110092T1 CY 20101100556 T CY20101100556 T CY 20101100556T CY 101100556 T CY101100556 T CY 101100556T CY 1110092 T1 CY1110092 T1 CY 1110092T1
Authority
CY
Cyprus
Prior art keywords
cumulative
culture
amino acid
glutamine
lactate
Prior art date
Application number
CY20101100556T
Other languages
English (en)
Inventor
Denis Drapeau
Yen-Tung Luan
James R Mercer
Wenge Wang
Daniel R Lasko
Original Assignee
Wyeth Research Ireland Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=35500648&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1110092(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wyeth Research Ireland Limited filed Critical Wyeth Research Ireland Limited
Publication of CY1110092T1 publication Critical patent/CY1110092T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions

Abstract

Παρέχεται βελτιωμένο σύστημα για παραγωγή μεγάλης κλίμακας του TNFR-Fc σε κυτταροκαλλιέργεια, ιδιαιτέρως σε μέσο που χαρακτηρίζεται από ένα ή περισσότερα από: i) αθροιστική συγκέντρωση αμινοξέος μεγαλύτερη από 70 mM, ii) γραμμομοριακή αθροιστική αναλογία αθροιστικής γλουταμίνης προς αθροιστική ασπαραγίνη μικρότερη από περίπου 2, iii) γραμμομοριακή αναλογία αθροιστικής γλουταμίνης προς αθροιστικό ολικό αμινοξύ μικρότερη από περίπου 0,2, iv) γραμμομοριακή αναλογία αθροιστικού ανόργανου ιόντος προς αθροιστικό ολικό αμινοξύ μεταξύ περίπου 0,4 έως 1 ή v) συνδυασμένη συγκέντρωση αθροιστικής γλουταμίνης και αθροιστικής ασπαραγίνης μεταξύ περίπου 16 και 36 mM. Η χρήση ενός τέτοιου συστήματος επιτρέπει υψηλά επίπεδα παραγωγής πρωτεΐνης και μειώνει την συσσώρευση ορισμένων ανεπιθύμητων παραγόντων, όπως αμμωνίου ή/και γαλακτικού. Επιπροσθέτως, παρέχονται μέθοδοι καλλιέργειας που συμπεριλαμβάνουν μετατόπιση θερμοκρασίας που τυπικώς περιλαμβάνει μία μείωση στην θερμοκρασία όταν η καλλιέργεια έχει φτάσει περίπου 20-80% της μεγίστης πυκνότητας κυττάρου αυτής. Εναλλακτικά ή επιπροσθέτως, η παρούσα μέθοδος παρέχει μεθόδους, έτσι ώστε μετά την επίτευξη μιας κορύφωσης, τα επίπεδα γαλακτικού ή/και αμμωνίου στην καλλιέργεια μειώνονται συναρτήσει του χρόνου.
CY20101100556T 2004-08-27 2010-06-16 ΠΑΡΑΣΚΕΥΗ TNFR-Fc CY1110092T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60537904P 2004-08-27 2004-08-27
EP08005732A EP1992697B1 (en) 2004-08-27 2005-08-26 Production of TNFR-Fc

Publications (1)

Publication Number Publication Date
CY1110092T1 true CY1110092T1 (el) 2015-01-14

Family

ID=35500648

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20101100022T CY1109721T1 (el) 2004-08-27 2010-01-08 Παρασκευη πρωτεινης συντηξης tnfr-lg
CY20101100556T CY1110092T1 (el) 2004-08-27 2010-06-16 ΠΑΡΑΣΚΕΥΗ TNFR-Fc

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CY20101100022T CY1109721T1 (el) 2004-08-27 2010-01-08 Παρασκευη πρωτεινης συντηξης tnfr-lg

Country Status (38)

Country Link
US (1) US7300773B2 (el)
EP (2) EP1781802B1 (el)
JP (3) JP2008511330A (el)
KR (1) KR100988451B1 (el)
CN (2) CN101061231B (el)
AR (2) AR050537A1 (el)
AT (2) ATE461285T1 (el)
AU (1) AU2005280036B2 (el)
BR (1) BRPI0514694B8 (el)
CA (1) CA2578138C (el)
CL (1) CL2017000577A1 (el)
CR (2) CR8998A (el)
CY (2) CY1109721T1 (el)
DE (2) DE602005020076D1 (el)
DK (2) DK1992697T3 (el)
EC (1) ECSP077354A (el)
EG (1) EG26922A (el)
ES (2) ES2341390T3 (el)
GT (2) GT200500233A (el)
HK (2) HK1121497A1 (el)
HN (1) HN2005000485A (el)
HR (2) HRP20100011T1 (el)
IL (1) IL181588A (el)
MX (1) MX2007002381A (el)
MY (1) MY137803A (el)
NO (1) NO344785B1 (el)
NZ (1) NZ579208A (el)
PE (2) PE20100448A1 (el)
PL (2) PL1781802T3 (el)
PT (2) PT1781802E (el)
RS (2) RS51255B (el)
RU (1) RU2458988C2 (el)
SI (2) SI1992697T1 (el)
SV (1) SV2006002211A (el)
TW (1) TWI364458B (el)
UA (1) UA89383C2 (el)
WO (1) WO2006026447A2 (el)
ZA (1) ZA200701672B (el)

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Publication number Publication date
CN101061231A (zh) 2007-10-24
KR100988451B1 (ko) 2010-10-18
MX2007002381A (es) 2007-06-15
TWI364458B (en) 2012-05-21
ES2341390T3 (es) 2010-06-18
MY137803A (en) 2009-03-31
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