CN116059303B

CN116059303B - Composite drynaria extract and preparation process thereof

Info

Publication number: CN116059303B
Application number: CN202310194957.3A
Authority: CN
Inventors: 斯聪聪; 庄培生; 孙振蛟; 王财林
Original assignee: Guangdong Qingyunshan Pharmaceutical Co ltd
Current assignee: Guangdong Qingyunshan Pharmaceutical Co ltd
Priority date: 2023-03-03
Filing date: 2023-03-03
Publication date: 2023-06-13
Anticipated expiration: 2043-03-03
Also published as: CN116059303A

Abstract

The invention relates to the field of plant extracts, and particularly discloses a composite drynaria rhizome extract and a preparation process thereof. The extraction process of the compound drynaria extract comprises the following steps: step 1), putting rhizoma drynariae, pinellia ternate, gladiolus, cardamom, euphorbia hirta and flos rosae sinensis into water, soaking for 1-2 hours, putting mixed bacterial liquid into the water, and soaking and extracting for 120-125 hours at the temperature of 35-36 ℃; step 2), heating to boiling, condensing and refluxing, and keeping boiling for 6-8h; step 3), filtering to obtain fermentation filtrate; step 4), heating and concentrating to obtain extract; step 5), adding deionized water for dissolution, filtering, and heating for concentration to obtain a fermentation concentrated solution, namely the composite drynaria extract; rhizoma Drynariae, rhizoma Pinelliae, rhizoma Acori Graminei, fructus Amomi rotundus, herba Euphorbiae Humifusae, and flos Hibisci Rosae-sinensis are sun-cured Chinese medicinal materials. The invention has the advantage of improving the effect of treating periodontitis.

Description

Composite drynaria extract and preparation process thereof

Technical Field

The invention relates to the field of plant extracts, in particular to a composite drynaria extract and a preparation process thereof.

Background

Rhizoma Drynariae is a plant of Gekko Swinhonis, and its rhizome can be used as medicine, and has effects of dispelling blood stasis, relieving pain, promoting reunion of bone, and treating toothache, lumbago, chronic diarrhea, etc. The study shows that the rhizoma drynariae has a certain effect on treating periodontitis, and the oral administration of the rhizoma drynariae can relieve periodontitis, diminish inflammation and relieve pain.

The effective substances in the drynaria rhizome are extracted and prepared into various dosage forms for taking, so that the nutritional ingredients in the drynaria rhizome can be absorbed more effectively, the troublesome process of decoction can be omitted, and the drynaria rhizome is more convenient, however, although the drynaria rhizome extract has the efficacy of treating periodontitis, the effect is more general, and research shows that even if the dosage is increased, the treatment effect is not obviously improved, and the discomfort caused by periodontitis to a patient is difficult to be relieved rapidly, so that the method has room for improvement.

Disclosure of Invention

In order to improve the effect of treating periodontitis, the application provides a compound drynaria rhizome extract and a preparation process thereof.

In a first aspect, the present application provides a process for extracting a compound drynaria extract, which adopts the following technical scheme:

an extraction process of a compound drynaria extract comprises the following steps:

step 1), putting rhizoma drynariae, pinellia ternate, gladiolus, cardamom, euphorbia hirta and flos rosae sinensis into water, soaking for 1-2 hours, putting mixed bacterial liquid into the water, and soaking and extracting for 120-125 hours at the temperature of 35-36 ℃;

step 2), heating to boiling, condensing and refluxing, and keeping boiling for 6-8h;

step 3), filtering to obtain fermentation filtrate;

step 4), heating and concentrating to obtain extract;

step 5), adding deionized water for dissolution, filtering, and heating for concentration to obtain a fermentation concentrated solution, namely the composite drynaria extract;

the rhizoma drynariae, pinellia tuber, gladiolus, cardamom, euphorbia hirta and hibiscus flower are all sun-cured traditional Chinese medicinal materials.

By adopting the technical scheme, the rhizoma drynariae, the pinellia ternate, the rhizoma anemones raddeanae, the cardamom, the euphorbia hirta and the hibiscus flower are compounded, so that the rhizoma drynariae, the pinellia ternate, the rhizoma anemones raddeanae, the cardamom, the euphorbia hirta and the hibiscus flower are matched in a synergistic way, and the prepared compound extract can quickly relieve pain, can more quickly reduce swelling, has stronger pertinence on periodontitis and has more obvious treatment effect after being taken.

Rhizoma Drynariae mainly contains naringin, methyl eugenol, new North America eriodictyoside, protocatechuic acid, and rhizoma Drynariae flavanone.

The pinellia tuber mainly contains amino acids, beta-sitosterol, pinellia ternate protein, alkaloid and other components.

Rhizoma anemones Altaicae mainly contains fatty acid, palmitic acid, succinic acid, 5-hydroxy levulinic acid, hexadecanoic acid, 9, 12-octadecadienoic acid, beta-sitosterol, anemonin, etc.

Fructus Amomi rotundus mainly contains alpha-terpineyl acetate, eucalyptol, limonene, alpha-dextran, etc.

The herba Euphorbiae Humifusae mainly contains flavonoid glycoside, phenols, triterpenes, and stems containing stanol, dandelion cyprohol, friedelane, beta-amyrin alcohol, beta-sitosterol, dandelion cyprone, myricetin, etc.

The flos Hibisci Rosae-sinensis mainly contains cyanidin-diglucoside, cyanidin sophoroglucose glucoside, quercetin diglucoside, and hibiscus sterol.

The experiments show that when naringin in rhizoma drynariae is matched with pinellia tuber, glabrous anemone rhizome, beta-sitosterol in euphorbia herb, limonene in cardamom, flavonoid glycoside in euphorbia herb and cornflower in hibiscus flower, the efficacy of treating periodontitis is obviously improved, especially the efficacy of relieving pain is quite obvious, and the treatment effect is better.

The compound drynaria extract of 0.3-0.4g is taken every day, so that the pain can be quickly relieved, the curative effect is very obvious after taking the compound drynaria extract for one month, the Gum Index (GI) is graded, the bacterial plaque index (PLI) is obviously reduced, the effect of quick treatment is achieved, and the life quality of patients is improved.

Preferably, in the step 1), the mass ratio of rhizoma drynariae, pinellia tuber, gladiolus, cardamom, euphorbia hirta and hibiscus flower is 8:0.5:0.4:0.6:0.3:0.2.

by adopting the technical scheme, the effect of treating periodontitis is better after the drynaria rhizome, the pinellia tuber, the gladiolus, the cardamom, the euphorbia hirta and the hibiscus flower are matched according to a specific proportion.

Preferably, in the step 5), ultrafiltration is performed by using an ultrafiltration membrane during filtration.

By adopting the technical scheme, the impurities can be effectively removed by adopting ultrafiltration membrane ultrafiltration, so that the drug effect of the compound extract is better.

Preferably, in the step 5), the mass of the fermentation concentrated solution is 18-22% of the total mass of the rhizoma drynariae, the pinellia tuber, the rhizoma anemones raddeanae, the cardamom, the euphorbia hirta and the hibiscus flower in the step 1) after heating and concentrating.

By adopting the technical scheme, the quality of the fermentation concentrated solution is controlled, the solvent ratio is reduced, the preparation is convenient to transport, and the preparation is prepared into a liquid preparation, so that split charging and taking are convenient, and meanwhile, the transportation is convenient.

Preferably, in the step 1), the preparation method of the mixed bacterial liquid comprises the following steps:

step 01), preparing a liquid culture medium;

step 02), inoculating aspergillus niger, propionibacterium freudenreichii, lactobacillus and geotrichum candidum into the liquid culture medium, and culturing until the OD value is more than or equal to 2, thus obtaining the mixed bacterial liquid.

By adopting the technical scheme, the composite strains of the aspergillus niger, the fisher propionic acid bacteria, the lactobacillus lactis and the geotrichum candidum are adopted to ferment and extract the drynaria, the pinellia ternate, the glabrous anemone rhizome, the cardamom, the euphorbia hirta and the hibiscus flowers, so that the cell walls of the medicinal materials are well decomposed, and the nutrient substances in the cells are easy to extract, so that the extract contains the active ingredients for treating periodontitis, the effect of treating periodontitis is better, part of saccharide substances can be decomposed, secretion which is nutritional to human bodies is generated, the immunity of the human bodies can be better improved after the extract is taken, and the recovery of the human bodies is facilitated to a certain extent, thereby the treatment effect of periodontitis is better.

In addition, the Aspergillus niger, the Fisher propionic acid bacteria, the lactobacillus and the Geotrichum candidum can play a role in promoting growth mutually, so that the activity of each bacterium is higher, cellulose can be decomposed more rapidly, the extraction efficiency is higher, and the method has higher economic value.

Through the culture of Aspergillus niger, fisher propionic acid bacterium, lactobacillus and Geotrichum candidum in liquid culture medium, the culture speed is faster and the economic value is higher due to the effect of promoting the growth mutually.

Preferably, in the step 01), the step of preparing the liquid medium is specifically as follows:

step 01-1), taking deionized water, carrots, balsam pears, sugarcanes, chrysanthemum and tea leaves, and crushing in a wall breaking machine to obtain wall breaking liquid;

step 01-2), adding starch into deionized water at normal temperature, stirring and dissolving, heating to gelatinize the starch, and cooling to room temperature to obtain thickening liquid;

and step 01-3), mixing the wall breaking liquid and the thickening liquid at room temperature to obtain the liquid culture medium.

Preferably, the mass ratio of deionized water, carrot, balsam pear, sugarcane, chrysanthemum and tea is as follows: 98:1:0.6:0.2:0.1:0.1.

by adopting the technical scheme, the growth and propagation of each strain can be better promoted by the culture of a special culture medium, enough strains can be quickly cultured, the activity of the strains can be screened, the high-activity strains can be reserved, the low-activity strains can be eliminated, the effect of simultaneously extracting the effective components in the drynaria, pinellia ternate, glabrous anemone rhizome, cardamom, euphorbia hirta and Chinese hibiscus flowers by the mixed bacterial liquid is better, and the nutritional components obtained by fermentation extraction have better curative effect on periodontitis.

Preferably, in the step 02), when aspergillus niger, fei-prinus, lactobacillus lactis and geotrichum candidum are inoculated, the mass ratio of the inoculum size of aspergillus niger, fei-prinus, lactobacillus lactis and geotrichum candidum is 1:3:4:2.

by adopting the technical scheme, the inoculation proportion of aspergillus niger, freudenreichii, lactobacillus lactis and geotrichum candidum is specifically selected, the synergistic growth effect is better, the concentration of each strain finally obtained can be controlled to a certain extent, the effect of simultaneous fermentation and extraction of rhizoma drynariae, pinellia ternate, rhizoma anemones raddeanae, cardamom, euphorbia hirta and hibiscus flower is better, the extraction efficiency is high, and the extract has better curative effect on periodontitis.

Preferably, in the step 02), after inoculating Aspergillus niger, phlebsiella oxytoca, lactobacillus lactis and Geotrichum candidum, culturing at 34-36 ℃.

By adopting the technical scheme, the activity of the strain is stimulated to the greatest extent by culturing at 34-36 ℃, the propagation efficiency is higher, the process time is shortened, and the economic benefit is improved.

Preferably, in the step 02), after inoculating Aspergillus niger, phlebsiella oxytoca, lactobacillus lactis and Geotrichum candidum, the liquid culture medium is placed in a magnetic field with a magnetic field strength of 600-700MT for cultivation.

By adopting the technical scheme, the strain with high reserved activity can be better screened through the cooperation of the special magnetic field strength and the special liquid culture medium, so that the fermentation and extraction effects of the mixed bacterial liquid are better, and the quality of the extract is better.

In a second aspect, the present application provides a compound drynaria extract, which adopts the following technical scheme:

a compound rhizoma Drynariae extract is prepared by the above extraction process.

By adopting the technical scheme, the extract has excellent curative effect on quality periodontitis, fast pain relieving, better quality effect and fast pain relieving of patients.

In summary, the present application has the following beneficial effects:

1. according to the preparation method, the drynaria rhizome, the pinellia tuber, the glabrous anemone rhizome, the cardamom, the euphorbia hirta and the hibiscus flower are compounded, so that the drynaria rhizome, the pinellia tuber, the glabrous anemone rhizome, the cardamom, the euphorbia hirta and the hibiscus flower are matched in a synergistic manner, the prepared compound extract can be used for rapidly relieving pain after being taken, can be used for relieving swelling more rapidly, has stronger pertinence on periodontitis and has more obvious treatment effect.

2. According to the method, the complex strains of the aspergillus niger, the freude propionic acid bacteria, the lactobacillus lactis and the geotrichum candidum are adopted to carry out fermentation extraction on the drynaria rhizome, the pinellia ternate, the gladiolus, the cardamom, the euphorbia hirta and the hibiscus flower, so that the cell walls of all medicinal materials are well decomposed, and nutrient substances in cells are easy to extract, so that the extract contains the active ingredients for treating periodontitis, the effect of treating periodontitis is better, part of saccharide substances can be decomposed, secretion which is nutritional to a human body is generated, the immunity of the human body can be better improved after the extract is taken, and the recovery of the human body is facilitated to a certain extent, so that the periodontitis treatment effect is better.

3. According to the method, through special culture medium culture, growth and propagation of various strains can be better promoted, enough strains can be quickly cultured, the activity of the strains can be screened, high-activity strains can be reserved, low-activity strains can be eliminated, the effect of simultaneously extracting active ingredients in rhizoma drynariae, pinellia ternate, rhizoma anemones raddeanae, cardamom, euphorbia hirta and flos rosae sinensis by mixed bacterial liquid is better, and nutritional ingredients obtained through fermentation extraction have better curative effects on treating periodontitis.

4. According to the method, the strain with high reserved activity can be better screened through the cooperation of the special magnetic field intensity and the special liquid culture medium, so that the fermentation and extraction effects of the mixed bacterial liquid are better, and the quality of the extract is better.

Detailed Description

The present application is described in further detail below with reference to examples.

Example 1

step 1), 8kg of drynaria rhizome, 0.5kg of pinellia tuber, 0.4kg of gladiolus, 0.6kg of cardamom, 0.3kg of euphorbia hirta and 0.2kg of hibiscus flower are weighed, mixed with 50kg of normal-temperature water, soaked in water for 1h, added with 1kg of mixed bacterial liquid, soaked and extracted at 35 ℃ for 125 hours to obtain an extracting solution.

And 2) heating the extracting solution to boiling, condensing and refluxing, and keeping boiling for 6 hours.

And 3) filtering through 200-mesh filter cloth to obtain fermentation filtrate.

And 4) heating and concentrating the fermentation filtrate to extract under the pressure of 0.01 MPa.

Step 5), adding 10kg of deionized water to dissolve the extractum, filtering through 200-mesh filter cloth, and heating and concentrating under 0.01MPa to obtain 1.8kg of fermentation concentrate, namely the compound drynaria extract.

Wherein rhizoma Drynariae, rhizoma Pinelliae, rhizoma Acori Graminei, fructus Amomi rotundus, herba Euphorbiae Humifusae, and flos Hibisci Rosae-sinensis are all commercial sun-cured Chinese medicinal materials.

In the step 1), the preparation method of the mixed bacterial liquid comprises the following steps:

step 01), preparing a liquid culture medium:

step 01-1), crushing 0.4kg deionized water, 0.01kg carrot, 0.006kg balsam pear, 0.002kg sugarcane, 0.001kg chrysanthemum and 0.001kg tea in a wall breaking machine to obtain wall breaking liquid.

Step 01-2), taking 0.5kg of deionized water, adding 0.08kg of starch at normal temperature, stirring for dissolution, heating to gelatinize the starch, and cooling to room temperature to obtain thickening liquid.

Step 02), inoculating 1mg of aspergillus niger, 3mg of freudenreichii, 4mg of lactobacillus and 2mg of geotrichum candidum into a liquid culture medium, and culturing at the constant temperature of 34 ℃ until the OD value is 2, thus obtaining a mixed bacterial liquid.

Example 2

step 1), 8kg of drynaria rhizome, 0.5kg of pinellia tuber, 0.4kg of gladiolus, 0.6kg of cardamom, 0.3kg of euphorbia hirta and 0.2kg of hibiscus flower are weighed, mixed with 50kg of normal-temperature water, soaked in water for 1h, added with 1kg of mixed bacterial liquid, soaked and extracted for 120 h at 36 ℃ to obtain an extracting solution.

And 2) heating the extracting solution to boiling, condensing and refluxing, and keeping boiling for 8 hours.

And 3) filtering through 200-mesh filter cloth to obtain fermentation filtrate.

And 5) adding 10kg of deionized water to dissolve the extractum, filtering through 200-mesh filter cloth, and heating and concentrating under 0.01MPa to obtain 2.2kg of fermentation concentrated solution, namely the compound drynaria extract.

step 01), preparing a liquid culture medium:

Step 02), inoculating 1mg of aspergillus niger, 3mg of freudenreichii, 4mg of lactobacillus and 2mg of geotrichum candidum into a liquid culture medium, and culturing at a constant temperature of 36 ℃ until the OD value is 2, thus obtaining a mixed bacterial liquid.

Example 3

step 1), 8kg of drynaria rhizome, 0.6kg of pinellia tuber, 0.3kg of gladiolus, 0.3kg of cardamom, 0.5kg of euphorbia herb and 0.3kg of hibiscus flower are weighed, mixed with 50kg of normal-temperature water, soaked in water for 1h, added with 1kg of mixed bacterial liquid, soaked and extracted at 35 ℃ for 125 hours to obtain an extracting solution.

And 3) filtering through 200-mesh filter cloth to obtain fermentation filtrate.

step 01), preparing a liquid culture medium:

Example 4

And 3) filtering through 200-mesh filter cloth to obtain fermentation filtrate.

Step 5), adding 10kg of deionized water to dissolve the extractum, filtering through 200-mesh filter cloth, and then ultrafiltering through an ultrafiltration membrane, wherein the molecular weight cut-off of the ultrafiltration membrane is 500D, the operating pressure is 1bar, and heating and concentrating under 0.01MPa to obtain 1.8kg of fermentation concentrate, namely the compound drynaria rhizome extract.

step 01), preparing a liquid culture medium:

Example 5

And 3) filtering through 200-mesh filter cloth to obtain fermentation filtrate.

step 01), preparing a liquid culture medium:

Step 02), inoculating 1mg of aspergillus niger, 3mg of freudenreichii, 4mg of lactobacillus and 2mg of geotrichum candidum into a liquid culture medium, placing the liquid culture medium in a magnetic field of 600MT, and culturing at the constant temperature of 34 ℃ until the OD value is 2, thus obtaining the mixed bacterial liquid.

Example 6

And 3) filtering through 200-mesh filter cloth to obtain fermentation filtrate.

step 01), preparing a liquid culture medium:

Step 02), inoculating 1mg of aspergillus niger, 3mg of freudenreichii, 4mg of lactobacillus lactis and 2mg of geotrichum candidum into a liquid culture medium, placing the liquid culture medium in a magnetic field of 700MT, and culturing at a constant temperature of 34 ℃ until the OD value is 2, thus obtaining the mixed bacterial liquid.

Example 7

The extraction process of the composite drynaria extract is different from that of the embodiment 1 only in that:

when preparing the liquid culture medium, the same amount of white radishes are used for replacing the carrots.

Example 8

when preparing the liquid culture medium, cucumber is adopted to replace balsam pear in equal quantity.

Example 9

when preparing the liquid culture medium, the onion is used for replacing the sugarcane in equal quantity.

Example 10

when the liquid culture medium is prepared, the flos lonicerae is adopted to replace the flos chrysanthemi with the same quantity.

Example 11

when preparing the liquid culture medium, the pericarpium citri reticulatae is adopted to replace tea leaves in equal quantity.

Example 12

when inoculating in liquid culture medium, the Aspergillus niger is replaced by Trichoderma viride in equal amount.

Example 13

bacillus licheniformis was used to replace the F.freudenreichii when inoculating in liquid medium.

Example 14

when inoculating in the liquid culture medium, the lactobacillus is replaced by bacillus subtilis.

Example 15

when inoculating in the liquid culture medium, the white geotrichum is replaced by the equal amount of the bud of the wax leaf.

Comparative example 1

in the step 1), rhizoma arisaematis is used for replacing rhizoma Pinelliae in equal amount.

Comparative example 2

in the step 1), the rhizoma acori graminei is replaced by the same amount.

Comparative example 3

in the step 1), the rhizoma atractylodis is adopted to replace the cardamom in an equivalent way.

Comparative example 4

in the step 1), purslane is adopted to replace the euphorbia hirta in an equivalent way.

Comparative example 5

in the step 1), the Hibiscus flower is replaced by the black nightshade in an equivalent way.

Experiment 1

The compound drynaria extract of each example and the compound drynaria extract of each comparative example are mixed with deionized water to prepare oral liquid, one bottle of oral liquid is 10ml, and each bottle of oral liquid contains 0.4g of compound drynaria extract.

A plurality of periodontitis patients are summoned, and one bottle of oral liquid is taken after breakfast and supper every day.

The treatment course is one month, and before taking, gum Index (GI) and plaque index (PLI) are measured, and are marked as initial GI and initial PLI, and after taking the oral liquid for one month, the oral liquid is measured again, and are marked as one month GI and one month PLI.

Gum index GI scoring criteria:

0 = gingival health;

1 = low gingival inflammation: the gum has slight change in color and slight edema, and bleeding is not detected;

2 = inflammation in gums: red gum, bright edema, and bleeding detection;

3 = severe inflammation of gums: the gums are visibly red and swollen or ulcerated and have a tendency to bleed automatically.

Plaque index PLI scoring criteria:

0 = gingival margin sterile plaque;

1 = thin plaque on the tooth surface at the gingival margin, but not visible in the visual inspection, if the tooth surface is scraped with a probe tip;

2 = moderate plaque visible at the gingival margin or adjacent surface;

3 = there is a lot of soft scale in the gingival sulcus or in the gingival margin and adjacent surfaces.

The toothache condition is detected every day in the course of treatment, and the administration days when the toothache completely disappears are recorded.

Every 10 persons were a group and all test values were averaged.

All patients had an initial GI of 3.

The initial PLI was 3 for all patients.

The specific test data for experiment 1 are detailed in table 1.

TABLE 1

According to the comparison of the data of the embodiment 1 and the data of the comparative examples 1-5 in the table 1, when the rhizoma drynariae, the pinellia ternate, the rhizoma anemones raddeanae, the cardamom, the euphorbia hirta and the hibiscus flower are compounded and extracted together, the compound rhizoma drynariae extract has a better effect of treating periodontitis, and after one month of treatment course, the gingiva can be recovered to mild inflammation, the dental plaque is obviously reduced, the curative effect is obvious, the toothache can be restrained after about 3 days, and the comfort level of patients is rapidly improved.

According to the comparison of the data of the embodiment 1 and the embodiment 5-6 in the table 1, the strain is cultivated by adding the magnetic field, and a certain screening effect is generated on the strain, so that the activity of the strain is higher, the fermentation extraction effect is better, and the timeliness of the obtained compound drynaria extract for inhibiting toothache is further improved.

According to the data comparison of the examples 7-11 in the example 1 in the table 1, when the strains are cultured by adopting ionized water, carrots, balsam pears, sugarcane, chrysanthemum and tea to prepare the culture medium, the activity of the cultured strains is higher, the fermentation extraction effect is better, special nutrition components can be generated in the culture medium, the nutrition components of the culture medium are added together when the strains are added, the timeliness of the prepared composite drynaria extract for inhibiting the toothache is further improved, the toothache is more quickly inhibited, and the comfort level of patients is improved.

According to the data comparison of examples 12-15 of example 1 in Table 1, when Aspergillus niger, fisher-Tropsch propionic acid bacteria, lactobacillus lactis and Geotrichum candidum are adopted for the matched fermentation extraction, the extraction effect on the complex system of rhizoma drynariae, pinellia ternate, rhizoma anemones Altaicae, cardamom, euphorbia hirta and Hibiscus flower is better, and the prepared complex rhizoma drynariae extract has obvious curative effect on periodontitis, obviously shortens the pain relieving time, improves the comfort level of patients faster and is more beneficial to treatment.

The present embodiment is merely illustrative of the present application and is not intended to be limiting, and those skilled in the art, after having read the present specification, may make modifications to the present embodiment without creative contribution as required, but is protected by patent laws within the scope of the claims of the present application.

Claims

1. A process for extracting a compound drynaria extract is characterized by comprising the following steps of: the method comprises the following steps:

step 3), filtering to obtain fermentation filtrate;

step 4), heating and concentrating to obtain extract;

the rhizoma drynariae, pinellia tuber, gladiolus, cardamom, euphorbia hirta and hibiscus flower are all sun-cured traditional Chinese medicinal materials;

step 01), preparing a liquid culture medium;

step 02), inoculating aspergillus niger, propionibacterium freudenreichii, lactobacillus and geotrichum candidum into a liquid culture medium, and culturing until the OD value is more than or equal to 2 to obtain a mixed bacterial liquid;

in the step 01), the step of preparing the liquid culture medium is specifically as follows:

2. The process for extracting a complex drynaria extract as claimed in claim 1, wherein: in the step 1), the mass ratio of the drynaria rhizome, the pinellia tuber, the gladiolus, the cardamom, the euphorbia hirta and the hibiscus flower is 8:0.5:0.4:0.6:0.3:0.2.

3. the process for extracting a complex drynaria extract as claimed in claim 1, wherein: in the step 5), ultrafiltration is performed by using an ultrafiltration membrane during filtration.

4. The process for extracting a complex drynaria extract as claimed in claim 1, wherein: in the step 5), heating and concentrating until the mass of the fermentation concentrated solution is 18-22% of the total mass of the rhizoma drynariae, the pinellia tuber, the rhizoma anemones raddeanae, the cardamom, the euphorbia hirta and the hibiscus flower in the step 1).

5. The process for extracting a complex drynaria extract as claimed in any one of claims 1 to 4, wherein: in the step 02), when aspergillus niger, propionibacterium freudenreichii, lactobacillus lactis and geotrichum candidum are inoculated, the mass ratio of the inoculation amount of the aspergillus niger, the propionibacterium freudenreichii, the lactobacillus lactis and the geotrichum candidum is 1:3:4:2.

6. the process for extracting a complex drynaria extract as claimed in claim 5, wherein: in the step 02), after Aspergillus niger, fisher propionic acid bacterium, lactobacillus and Geotrichum candidum are inoculated, the culture is carried out at the constant temperature of 34-36 ℃.

7. The process for extracting a complex drynaria extract as claimed in claim 6, wherein: in the step 02), after Aspergillus niger, fisher propionic acid bacterium, lactobacillus and Geotrichum candidum are inoculated, the liquid culture medium is placed in a magnetic field with the magnetic field strength of 600-700MT for culture.

8. A compound drynaria extract, which is characterized in that: is prepared by the extraction process of the compound drynaria rhizome extract as claimed in any one of claims 1 to 7.