CN116035978B - Plant extract with red dispelling effect and preparation method and application thereof - Google Patents
Plant extract with red dispelling effect and preparation method and application thereof Download PDFInfo
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- CN116035978B CN116035978B CN202310125558.1A CN202310125558A CN116035978B CN 116035978 B CN116035978 B CN 116035978B CN 202310125558 A CN202310125558 A CN 202310125558A CN 116035978 B CN116035978 B CN 116035978B
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- plant extract
- kuh
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the technical field of skin care products, in particular to a plant extract with a red dispelling effect, a preparation method and application thereof. The plant extract with the red dispelling effect comprises the following components in parts by mass: 1.5 to 6 parts of kuh-seng root extract, 1 to 4 parts of tribulus fruit extract and 0.5 to 2 parts of dogwood fruit extract. The invention provides a plant extract, which contains kuh-seng root extract, tribulus fruit extract and dogwood fruit extract, and can inhibit Ca 2+ The multi-dimensional effects of channel activation, inhibition of vasodilation factor (PGE-2), inhibition of inflammatory mediators and the like solve the problem of skin redness, and achieve the effect of rapidly removing the red.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to a plant extract with a red dispelling effect, a preparation method and application thereof.
Background
Sensitive muscle refers to a state of hyperreactivity of the skin under physiological or pathological conditions, usually in the face, and the inducing factors may be physical, chemical or neuropsychiatric. The sensitive muscles commonly show that: itching, tingling, redness, tightness, desquamation, etc., and it has been found that the most plagued skin symptom of severely sensitive patients is facial redness, so that there is an urgent need for a cosmetic raw material capable of "fast and long-acting" solving the facial redness and clear passage. However, the plant extract for effectively removing red at the cosmetic end is not much, and bisabolol is usually adopted, the component is an oil-soluble component, and the efficacy target is on an anti-inflammatory target. Moreover, the action path of the single plant extract is relatively narrow, which is unfavorable for comprehensively and rapidly solving the skin problem.
In view of this, the present invention has been made.
Disclosure of Invention
An object of the present invention is to provide a plant extract having a redness-removing effect, which can solve the problem of skin redness from a multi-dimensional aspect.
Another object of the present invention is to provide a method for preparing a plant extract having a red dispelling effect.
It is still another object of the present invention to provide the use of a plant extract having a red dispelling effect in the preparation of a skin care product.
In order to achieve the above purpose, the invention provides a plant extract with red dispelling effect, which comprises the following components in parts by mass:
1.5 to 6 parts of kuh-seng root extract, 1 to 4 parts of tribulus fruit extract and 0.5 to 2 parts of dogwood fruit extract.
In a specific embodiment of the invention, the plant extract comprises the following components in parts by mass: 3 parts of kuh-seng root extract, 2 parts of tribulus fruit extract and 1 part of dogwood fruit extract.
In a specific embodiment of the invention, the plant extract further comprises 85-95 parts of butanediol and 2-6 parts of water in parts by weight. Further, the plant extract also comprises 90 parts of butanediol and 4 parts of water in parts by weight.
In a specific embodiment of the present invention, the kuh-seng extract is an aqueous ethanol extract of kuh-seng; the tribulus fruit extract is an ethanol water solution extract of tribulus fruit; the cornel fruit extract is ethanol water solution extract of cornel fruit. Further, in the ethanol aqueous solution, the volume fraction of ethanol is 90% -95%.
In another aspect, the present invention provides a method for preparing a plant extract having a red dispelling effect, comprising the steps of:
ethanol water solution is used as an extraction solvent, the ratio of feed to liquid is 1:10-1:30, and the kuh-seng, the tribulus fruit and the dogwood fruit are extracted for 1-3 h at 65-85 ℃.
In a specific embodiment of the present invention, further comprising: and fumigating the kuh-seng, the tribulus fruit and the dogwood fruit before the extraction.
In a specific embodiment of the present invention, further comprising: concentrating the extracted liquid, and then redissolving by adopting 1, 3-butanediol.
In a specific embodiment of the present invention, the reconstitution comprises: 1, 3-butanediol was added in portions and concentrated after each addition. Further, the 1, 3-butanediol is added in at least 2 batches, such as 3-5 batches.
In a specific embodiment of the invention, the volume fraction of ethanol in the ethanol aqueous solution is 90% -95%.
In a specific embodiment of the present invention, the ratio of the feed liquid is 1:15 to 1:20.
In a specific embodiment of the invention, the extraction temperature is 75-85 ℃.
In a specific embodiment of the invention, the extraction time is 2-3 hours.
In a specific embodiment of the present invention, the mass ratio of the kuh-seng, the tribulus fruit and the dogwood fruit is (15-60)/(10-40)/(5-20), such as 3:2:1.
In a specific embodiment of the present invention, in the reconstitution, the 1, 3-butanediol is used in an amount of 10 to 25 times by weight of the crude drug based on the amount of the crude drug. Further, the dosage of the 1, 3-butanediol is 10-16 times of the weight of the crude drug.
In still another aspect, the invention provides an application of any one of the plant extracts with the red dispelling effect in preparing skin care products.
In a specific embodiment of the invention, the skin care product comprises a skin care product for sensitive muscles.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention provides a plant extract, which contains kuh-seng root extract, tribulus fruit extract and dogwood fruit extract, and can inhibit Ca 2+ The multi-dimensional effects of channel activation, inhibition of vasodilation factors, inhibition of inflammatory mediators and the like solve the problem of skin redness, and achieve the effect of rapidly dispelling the red;
(2) The preparation method of the plant extract can improve the content of active ingredients, reduce ethanol residues and obviously improve the red dispelling effect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 shows the results of the stimulation test of the chick embryo with the plant extracts of examples 1 and 33 of the present invention; wherein the left plot corresponds to the chick embryo after the plant extract of example 33 was acted upon, and the right plot corresponds to the chick embryo after the plant extract of example 1 was acted upon;
FIG. 2 is a graph showing the average value of TiVi-index of skin sensitivity in the test of the red-removing efficacy of the plant extract gel sample provided in example 1 of the present invention;
FIG. 3 is a graph showing the average rate of change of the skin sensitivity TiVi-index relative to the initial value in the test of the red-dispelling efficacy of the plant extract gel sample provided in example 1 of the present invention;
fig. 4 is a view showing the TiVi images of the plant extract gel sample provided in example 1 of the present invention before and after use at different times;
fig. 5 shows the skin color a value test results in the red-removing effect test of the plant extract gel samples provided in examples 23 to 26, example 33 and comparative examples 1 to 2 of the present invention;
FIG. 6 is a mean change rate test result of the initial values of the skin sensitivity TiVi-index in the red-removing efficacy test of the plant extract gel samples provided in examples 23 to 26, example 33 and comparative examples 1 to 2 according to the present invention;
FIG. 7 is a graph showing the results of the calcium flux test of the plant extract provided in example 1 of the present invention;
FIG. 8 is a graph showing the results of an inhibition test of UV-induced inflammatory factor TNF- α by the plant extract provided in example 1 of the present invention;
FIG. 9 is a graph showing the results of an inhibition test of LPS-induced IL-8 by the plant extract according to example 1 of the present invention;
FIG. 10 is a graph showing the results of an inhibition test of UV-induced PGE2 by the plant extract according to example 1 of the present invention;
FIG. 11 is a graph showing the results of UV-induced COX-2 inhibition assay test for plant extracts provided in example 1 of the present invention.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings and detailed description, but it will be understood by those skilled in the art that the examples described below are some, but not all, examples of the present invention, and are intended to be illustrative of the present invention only and should not be construed as limiting the scope of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
The invention provides a plant extract with a red dispelling effect, which comprises the following components in parts by mass:
1.5 to 6 parts of kuh-seng root extract, 1 to 4 parts of tribulus fruit extract and 0.5 to 2 parts of dogwood fruit extract.
In various embodiments, the plant extract with red dispelling effect may include the following components in parts by weight:
the radix Sophorae Flavescentis extract can be 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc.;
the fructus Tribuli extract can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, etc.;
the dogwood fruit extract may be 0.5 part, 0.8 part, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, etc.
In the plant extract of the invention, the amounts of the kuh-seng root extract, the tribulus fruit extract and the dogwood fruit extract are calculated by the amounts of the used raw materials of the kuh-seng root, the tribulus fruit and the dogwood fruit respectively.
The radix Sophorae Flavescentis is root of Sophora flavescens ait of Leguminosae, contains multiple alkaloids such as matrine, oxymatrine, sophoroalcohol, and cytisine, has antibacterial, antiinflammatory, antiallergic, and immunity regulating effects, and is mainly used for treating dermatoses such as eczema and pruritus due to wind heat and damp heat. Fructus Tribuli, bitter and pungent in taste, warm in nature, has effects of suppressing hyperactive liver, resolving stagnation, improving eyesight, relieving itching, etc. The chemical components of the caltrop mainly comprise saponins, flavonoids, alkaloids and the like, wherein the saponins are main active components. Modern pharmacological researches have found that the tribulus saponins have multiple effects of resisting atherosclerosis, resisting oxidization, improving cardiac function and the like. The main chemical components of the dogwood include iridoid and glycosides, triterpenes, flavonoids, tannins, organic acids and the like, and the dogwood has various physiological activities such as neuroprotection, anti-inflammation, hypoglycemic, bacteriostasis, anti-tumor, liver protection and the like. The invention adopts a certain amount of kuh-seng root extract, tribulus fruit extract and dogwood fruit extract to compound, and can inhibit Ca 2+ The multi-dimensional effects of channel activation, inhibition of vasodilation factors, inhibition of inflammatory mediators and the like solve the problem of skin redness, and achieve the effect of rapidly dispelling the red.
In a specific embodiment of the invention, the plant extract comprises the following components in parts by mass: 3 parts of kuh-seng root extract, 2 parts of tribulus fruit extract and 1 part of dogwood fruit extract.
In a specific embodiment of the invention, the plant extract further comprises 85-95 parts by weight of 1, 3-butanediol and 2-6 parts by weight of water. Further, the plant extract also comprises 90 parts of 1, 3-butanediol and 4 parts of water in parts by weight.
As in the various embodiments, the mass parts of 1, 3-butandiol and water in the plant extract may be exemplified as follows: the 1, 3-butanediol may be 85 parts, 86 parts, 87 parts, 88 parts, 89 parts, 90 parts, 91 parts, 92 parts, 93 parts, 94 parts, 95 parts, etc.; the water may be 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc.
In a specific embodiment of the present invention, the kuh-seng extract is an aqueous ethanol extract of kuh-seng; the tribulus fruit extract is an ethanol water solution extract of tribulus fruit; the cornel fruit extract is ethanol water solution extract of cornel fruit. Further, in the ethanol aqueous solution, the volume fraction of ethanol is 90% -95%.
As in the various embodiments, the volume fraction of ethanol in the aqueous ethanol solution may be illustratively 90%, 91%, 92%, 93%, 94%, 95%, etc.
In a specific embodiment of the present invention, the plant extract comprises flavone and matrine oxide in a mass ratio of (1.3-2.0) to 1, such as (1.55-1.6) to 1.
In various embodiments, the mass ratio of flavone to matrine in the plant extract may be exemplified by 1.3:1, 1.35:1, 1.4:1, 1.45:1, 1.5:1, 1.55:1, 1.6:1, 1.65:1, 1.7:1, 1.75:1, 1.8:1, 1.85:1, 1.9:1, 2.0:1, etc.
In another aspect, the present invention provides a method for preparing a plant extract having a red dispelling effect, comprising the steps of: ethanol water solution is used as an extraction solvent, the ratio of feed to liquid is 1:10-1:30, and the kuh-seng, the tribulus fruit and the dogwood fruit are extracted for 1-3 h at 65-85 ℃.
In various embodiments, the feed liquid ratio may be exemplified by 1:10 (m/m), 1:12 (m/m), 1:15 (m/m), 1:18 (m/m), 1:20 (m/m), 1:22 (m/m), 1:25 (m/m), 1:28 (m/m), 1:30 (m/m), and the like. Wherein the feed to liquid ratio refers to the ratio of dried plant material to extraction solvent without pretreatment (e.g., fumigation).
As in the various embodiments, the temperature of extraction may be illustratively 65 ℃, 70 ℃, 75 ℃, 80 ℃, 85 ℃, etc.; the extraction time may be exemplified by 1h, 1.5h, 2h, 2.5h, 3h, etc.
In a specific embodiment of the present invention, further comprising: and fumigating the kuh-seng, the tribulus fruit and the dogwood fruit before the extraction. Further, the fumigation treatment time is more than or equal to 20min, such as 20-40 min.
As in the various embodiments, the fumigation treatment time may be exemplified by 20min, 25min, 30min, 35min, 40min, etc. Wherein the fumigation means fumigation by adopting steam.
In a specific embodiment of the present invention, further comprising: concentrating the extracted liquid, and then redissolving by adopting 1, 3-butanediol.
In actual operation, the extracted material is cooled to room temperature, and is filtered by gauze to obtain an extracting solution. The filtering mode can be adjusted according to actual conditions.
In a specific embodiment of the present invention, the reconstitution comprises: 1, 3-butanediol was added in portions and concentrated after each addition. Further, the 1, 3-butanediol is added in at least 2 batches, such as 3-5 batches.
As in the various embodiments, the 1, 3-butanediol may be added in 2, 3, 4, or 5 batches.
In practice, the manner of the concentration process may be selected according to conventional operations, such as concentration with negative pressure.
In a specific embodiment of the invention, in the reconstitution, the amount of 1, 3-butanediol added in a single batch is 15% -25% of the total amount of 1, 3-butanediol.
As in the various embodiments, the amount of 1, 3-butanediol added in a single batch in the reconstitution may illustratively be 15%, 18%, 20%, 22%, 25% of the total amount of 1, 3-butanediol, etc.
In a specific embodiment of the invention, the volume fraction of ethanol in the ethanol aqueous solution is 90% -95%.
As in the various embodiments, the volume fraction of ethanol in the aqueous ethanol solution may be illustratively 90%, 91%, 92%, 93%, 94%, 95%, etc.
By adopting the extraction solvent, the effective components can be effectively extracted, and the plant extract with the effect of removing red is obtained.
In a specific embodiment of the present invention, the ratio of the liquid to the gas is 1:15 to 1:20, for example, the ratio of the liquid to the gas is 1:20.
In a specific embodiment of the invention, the extraction temperature is 75-85 ℃, e.g., 75 ℃.
In a specific embodiment of the invention, the extraction time is 2-3 hours, such as 2 hours.
In a specific embodiment of the present invention, the mass ratio of the kuh-seng, the tribulus fruit and the dogwood fruit is (15-60)/(10-40)/(5-20), such as 3:2:1.
In various embodiments, the mass ratio of the kuh-seng, the fructus Tribuli, and the fructus Corni may be exemplified by 3:2:1, 3:3:1, 1:1:1, 2:3:1, 2:3:2, 2:2:1, 3:3:2:2, and the like.
In a specific embodiment of the present invention, in the reconstitution, the 1, 3-butanediol is used in an amount of 10 to 25 times by weight of the crude drug based on the amount of the crude drug. Further, the dosage of the 1, 3-butanediol is 10-16 times of the weight of the crude drug.
As in the various embodiments, the amount of 1, 3-butanediol in the reconstitution may be exemplified by 10 times, 11 times, 12 times, 13 times, 14 times, 15 times, 16 times, 17 times, 18 times, 19 times, 20 times, 21 times, 22 times, 23 times, 24 times, 25 times, etc., preferably 16 times, the weight of the drug substance, based on the amount of the crude drug.
In still another aspect, the invention provides an application of any one of the plant extracts with the red dispelling effect in preparing skin care products.
In a specific embodiment of the invention, the skin care product comprises a skin care product for sensitive muscles.
For example, the plant extract with red dispelling effect is used for preparing Ca inhibiting agent 2+ Application of skin care products for activating channels, inhibiting vasodilation factors and/or inhibiting expression of inflammatory mediatorsIs used.
The plant extract can comprehensively and rapidly solve the problem of Pi Fufan red through multiple paths and multiple targets.
In the following examples and comparative examples, if not specifically described, the raw materials of kuh-seng, tribulus fruit and cornus officinalis are used as coarse powder, and the coarse powder is sieved with a 6-mesh sieve to obtain the undersize product.
Examples 1 to 7
Examples 1 to 7 provide a method for preparing a plant extract having a red dispelling effect, comprising the steps of:
(1) Weighing radix Sophorae Flavescentis, fructus Tribuli and Corni fructus according to a certain proportion, adding water into steamer, boiling, and fumigating radix Sophorae Flavescentis, fructus Tribuli and Corni fructus in steamer for 20min;
(2) Adding 95% ethanol water solution according to a feed liquid ratio of 1:20, and extracting at 75deg.C for 2 hr;
(3) Cooling the extracted material to room temperature, and filtering with gauze to obtain extractive solution; then placing the extracting solution in a rotary steaming bottle, and concentrating under reduced pressure until the extracting solution is hung on the wall to obtain concentrated solution;
(4) Adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution; in the redissolution, 1, 3-butanediol is added for 5 times, 20% of the total amount of the 1, 3-butanediol is added each time, the materials are transferred into a rotary steaming bottle after each addition, and the materials are concentrated under reduced pressure until the quality is constant;
(5) Cooling the concentrated solution obtained in the step (4) to below 35 ℃, and filtering by using H70 paper board; sterilizing the filtrate at 80-85 ℃ for 40min, cooling and uniformly mixing to obtain the plant extract with the effect of removing red.
The mass ratio of the kuh-seng root, the tribulus fruit and the dogwood fruit adopted in the preparation method of examples 1-7 is shown in table 1, and the flavone content (rutin standard) in the obtained plant extract is shown in the table 1.
Table 1 comparison of different raw material ratios
Name of the name | Raw material proportioning | Flavone content (mg/mL) |
Example 1 | Kuh-seng root, tribulus fruit, dogwood fruit and 3:2:1 | 0.609 |
Example 2 | Kuh-seng root, tribulus fruit, dogwood fruit=3:3:1 | 0.554 |
Example 3 | Kuh-seng root, tribulus fruit, dogwood fruit=1:1:1 | 0.368 |
Example 4 | Kuh-seng root, tribulus fruit, dogwood fruit, 2, 3 and 1 | 0.428 |
Example 5 | Kuh-seng root, tribulus fruit, dogwood fruit=2:3:2 | 0.507 |
Example 6 | Kuh-seng root, tribulus fruit, dogwood fruit=2:2:1 | 0.461 |
Example 7 | Kuh-seng root:Tribulus terrestris fruitFructus Corni=3:3:2 | 0.539 |
Examples 8 to 9
Examples 8 to 9 provide a method for preparing a plant extract having a red dispelling effect, comprising the steps of:
(1) Weighing radix Sophorae Flavescentis, fructus Tribuli and Corni fructus according to mass ratio of 3:2:1, adding water into steamer, boiling, and fumigating radix Sophorae Flavescentis, fructus Tribuli and Corni fructus in steamer for 20min;
(2) Adding 95% ethanol water solution according to a certain feed-liquid ratio, and extracting at 75deg.C for 2 hr;
(3) Cooling the extracted material to room temperature, and filtering with gauze to obtain extractive solution; then placing the extracting solution in a rotary steaming bottle, and concentrating under reduced pressure until the extracting solution is hung on the wall to obtain concentrated solution;
(4) Adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution; in the redissolution, 1, 3-butanediol is added for 5 times, 20% of the total amount of the 1, 3-butanediol is added each time, the materials are transferred into a rotary steaming bottle after each addition, and the materials are concentrated under reduced pressure until the quality is constant;
(5) Cooling the concentrated solution obtained in the step (4) to below 35 ℃, and filtering by using H70 paper board; sterilizing the filtrate at 80-85 ℃ for 40min, cooling and uniformly mixing to obtain the plant extract with the effect of removing red.
The specific feed ratios used in the preparation methods of examples 1, 8 and 9 and the flavone content (rutin scale) in the obtained plant extracts are shown in Table 2.
TABLE 2 comparison of different feed ratios
Name of the name | Feed liquid ratio (m: m) | Flavone content (mg/mL) |
Example 8 | 1﹕10 | 0.568 |
Example 1 | 1﹕20 | 0.609 |
Example 9 | 1﹕30 | 0.582 |
Examples 10 to 13
Examples 10 to 13 provide a method for preparing a plant extract having a red dispelling effect, comprising the steps of:
(1) Weighing radix Sophorae Flavescentis, fructus Tribuli and Corni fructus according to mass ratio of 3:2:1, adding water into steamer, boiling, and fumigating radix Sophorae Flavescentis, fructus Tribuli and Corni fructus in steamer for 20min;
(2) Adding 95% ethanol water solution according to a feed liquid ratio of 1:20 into the fumigated material, and extracting for 1-3 h at 75 ℃;
(3) Cooling the extracted material to room temperature, and filtering with gauze to obtain extractive solution; then placing the extracting solution in a rotary steaming bottle, and concentrating under reduced pressure until the extracting solution is hung on the wall to obtain concentrated solution;
(4) Adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution; in the redissolution, 1, 3-butanediol is added for 5 times, 20% of the total amount of the 1, 3-butanediol is added each time, the materials are transferred into a rotary steaming bottle after each addition, and the materials are concentrated under reduced pressure until the quality is constant;
(5) Cooling the concentrated solution obtained in the step (4) to below 35 ℃, and filtering by using H70 paper board; sterilizing the filtrate at 80-85 ℃ for 40min, cooling and uniformly mixing to obtain the plant extract with the effect of removing red.
The extraction times used in the preparation methods of examples 1, 10 to 13 are shown in Table 3, and the flavone content (rutin scale) in the obtained plant extracts.
TABLE 3 comparison of different extraction times
Examples 14 to 17
Examples 14 to 17 provide a method for preparing a plant extract having a red dispelling effect, comprising the steps of:
(1) Weighing radix Sophorae Flavescentis, fructus Tribuli and Corni fructus according to mass ratio of 3:2:1, adding water into steamer, boiling, and fumigating radix Sophorae Flavescentis, fructus Tribuli and Corni fructus in steamer for 20min;
(2) Adding 95% ethanol water solution according to a feed liquid ratio of 1:20 into the fumigated material, and extracting for 2h at 65-85 ℃;
(3) Cooling the extracted material to room temperature, and filtering with gauze to obtain extractive solution; then placing the extracting solution in a rotary steaming bottle, and concentrating under reduced pressure until the extracting solution is hung on the wall to obtain concentrated solution;
(4) Adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution; in the redissolution, 1, 3-butanediol is added for 5 times, 20% of the total amount of the 1, 3-butanediol is added each time, the materials are transferred into a rotary steaming bottle after each addition, and the materials are concentrated under reduced pressure until the quality is constant;
(5) Cooling the concentrated solution obtained in the step (4) to below 35 ℃, and filtering by using H70 paper board; sterilizing the filtrate at 80-85 ℃ for 40min, cooling and uniformly mixing to obtain the plant extract with the effect of removing red.
The extraction temperatures used in the preparation methods of examples 1, 14 to 17 are shown in Table 4, and the flavone content (rutin scale) in the obtained plant extracts is shown in Table 4.
TABLE 4 comparison of different extraction temperatures
Name of the name | Extraction temperature | Flavone content (mg/mL) |
Example 14 | 65℃ | 0.491 |
Example 15 | 70℃ | 0.528 |
Example 1 | 75℃ | 0.609 |
Example 16 | 80℃ | 0.606 |
Example 17 | 85℃ | 0.607 |
Examples 18 to 22
Examples 18 to 22 provide a method for preparing a plant extract having a red dispelling effect, comprising the steps of:
(1) Weighing radix Sophorae Flavescentis, fructus Tribuli and Corni fructus according to mass ratio of 3:2:1, adding water into steamer, boiling, and fumigating radix Sophorae Flavescentis, fructus Tribuli and Corni fructus in steamer for 20min;
(2) Adding 95% ethanol water solution according to a feed liquid ratio of 1:20, and extracting at 75deg.C for 2 hr;
(3) Cooling the extracted material to room temperature, and filtering with gauze to obtain extractive solution; then placing the extracting solution in a rotary steaming bottle, and concentrating under reduced pressure until the extracting solution is hung on the wall to obtain concentrated solution;
(4) Adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to a certain crude drug amount for redissolution; in the redissolution, 1, 3-butanediol is added for 5 times, 20% of the total amount of the 1, 3-butanediol is added each time, the materials are transferred into a rotary steaming bottle after each addition, and the materials are concentrated under reduced pressure until the quality is constant;
(5) Cooling the concentrated solution obtained in the step (4) to below 35 ℃, and filtering by using H70 paper board; sterilizing the filtrate at 80-85 ℃ for 40min, cooling and uniformly mixing to obtain the plant extract with the effect of removing red.
The amounts of 1, 3-butanediol used in the preparation methods of examples 1 and 18 to 22 are shown in Table 5, and the flavone content (rutin scale) in the obtained plant extracts.
TABLE 5 comparison of different reconstitution ratios
Name of the name | Reconstitution ratio (calculated from crude drug, concentrate to solvent) | Flavone content (mg/mL) |
Example 18 | 1﹕10 | 0.588 |
Example 19 | 1﹕13 | 0.554 |
Example 1 | 1﹕16 | 0.609 |
Example 20 | 1﹕19 | 0.504 |
Example 21 | 1﹕22 | 0.456 |
Example 22 | 1﹕25 | 0.425 |
Example 23
This example refers to the preparation method of example 1, differing only in: this example does not include the fumigation treatment in step (1) of example 1.
Example 24
This example refers to the preparation method of example 1, differing only in: this example does not include the fumigation treatment in step (1) of example 1; and this example uses an equal weight of propylene glycol to replace the re-solvent 1, 3-butanediol of example 1.
Example 25
This example refers to the preparation method of example 1, differing only in: this example does not include the fumigation treatment in step (1) of example 1, but the material is placed in an autoclave for 20 minutes at a micro pressure (0.1 MPa).
Example 26
This example refers to the preparation method of example 25, which differs only in that: step (3) is different.
Step (3) of this embodiment is: cooling the extracted material to room temperature, and filtering with gauze to obtain extractive solution; adding 2wt% (based on the mass of the extract) of activated carbon, deodorizing at 60 ℃ for 1h, filtering by using F100 paper board, and collecting filtrate; and (3) placing the filtrate in a rotary steaming bottle, and concentrating under reduced pressure until the wall is built up to obtain concentrated solution.
The color, appearance, smell and corresponding physicochemical index of the products obtained in example 1 and examples 24 to 26 were recorded and tested, and the results are shown in Table 6.
TABLE 6 results of product testing for different examples
Examples 27 to 30
The embodiment provides a preparation method of a plant extract with a red dispelling effect, which comprises the following steps:
(1) Weighing radix Sophorae Flavescentis, fructus Tribuli and Corni fructus according to mass ratio of 3:2:1, adding water into steamer, boiling, and fumigating radix Sophorae Flavescentis, fructus Tribuli and Corni fructus in steamer for a certain time;
(2) Adding 95% ethanol water solution according to a feed liquid ratio of 1:20, and extracting at 75deg.C for 2 hr;
(3) Cooling the extracted material to room temperature, and filtering with gauze to obtain extractive solution; then placing the extracting solution in a rotary steaming bottle, and concentrating under reduced pressure until the extracting solution is hung on the wall to obtain concentrated solution;
(4) Adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution; in the redissolution, 1, 3-butanediol is added for 5 times, 20% of the total amount of the 1, 3-butanediol is added each time, the materials are transferred into a rotary steaming bottle after each addition, and the materials are concentrated under reduced pressure until the quality is constant;
(5) Cooling the concentrated solution obtained in the step (4) to below 35 ℃, and filtering by using H70 paper board; sterilizing the filtrate at 80-85 ℃ for 40min, cooling and uniformly mixing to obtain the plant extract with the effect of removing red.
The fumigation times specifically adopted in the preparation methods of example 1, example 23 and examples 27 to 30 are shown in Table 7, and the content of matrine oxide in the obtained plant extracts.
TABLE 7 comparison of different fumigation times
Name of the name | Fumigating time/min | Matrine oxide content (mg/mL) |
Example 23 | 0 | 0.171 |
Example 27 | 5 | 0.235 |
Example 28 | 10 | 0.261 |
Example 29 | 15 | 0.298 |
Example 1 | 20 | 0.387 |
Example 30 | 40 | 0.385 |
Example 31
This example refers to the preparation method of example 1, differing only in: step (4) is different.
Step (4) of this embodiment is: adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution; in the redissolution, 1, 3-butanediol is added for 2 times, 70% is added for the first time, 30% is added for the second time, and the materials are transferred into a rotary steaming bottle after each addition, and are concentrated under reduced pressure until the quality is constant.
Example 32
This example refers to the preparation method of example 1, differing only in: step (4) is different.
Step (4) of this embodiment is: adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution; in the redissolution, 1, 3-butanediol is added for 2 times, 50% is added for the first time, 50% is added for the second time, and the materials are transferred into a rotary steaming bottle after each addition, and are concentrated under reduced pressure until the quality is constant.
Example 33
This example refers to the preparation method of example 1, differing only in: step (4) is different.
Step (4) of this embodiment is: and (3) adding 1, 3-butanediol into the concentrated solution obtained in the step (3) according to the amount of 16 times of crude drugs for redissolution, transferring the materials into a rotary steaming bottle, and concentrating under reduced pressure until the quality is constant.
The ethanol content of the plant extracts obtained by the preparation methods of example 1 and examples 31 to 33 are shown in Table 8. The ethanol content test method refers to gas chromatography-mass spectrometry in chapter 2.33 of cosmetic safety technical Specification (2015).
TABLE 8 comparison of different reconstitution treatments
The samples of example 1 and example 33 were tested for their stimulating effect on chick embryos and the test results are shown in FIG. 1. As can be seen from the figure, the sample of example 33 has a stimulating effect on chick embryos and is dissolved in capillaries; whereas the sample of example 1 was not found to have a stimulating effect on chick embryos.
The test method comprises the following steps:
1. test group setup
(1) Solvent control group: physiological saline, i.e. 0.9% aqueous nacl.
(2) Model control group: 0.1g of SDS was weighed out into 9.9g of physiological saline, and the mixture was vortexed and mixed well.
(3) Negative control group: 0.1% SDS, 10-fold dilution from 1% SDS.
(4) Sample group: diluting the sample to 20% with physiological saline; 2mL of SDS (0.2% of SDS) is added into the mixture, and the mixture is uniformly mixed by vortex to obtain the final concentration of 10%.
2. Test procedure
(1) CAM preparation: the chick embryo was cultivated to 9 days old, checked with an egg candling, eggshells of the air chamber were peeled off with dental saw tooth bent forceps, white egg membranes were exposed, and careful handling did not compromise the integrity of the egg membranes. A few drops of physiological saline solution are dripped by a suction pipe to moisten eggshell membranes, and forceps are carefully used for removing the eggshell membranes, so that the vascular membranes are not damaged. At this point again the structure of the vascular system is observed and a determination is made as to its integrity and suitability for testing.
(2) The end point evaluation method is operated: taking 0.3mL of the test object to directly act on the CAM, taking a picture to observe and record the change degree of the vascular toxicity effect after 3min of the action, namely the degree of vascular bleeding, coagulation and vascular thawing.
Each test was provided with 6 chick embryos, 6 chick embryos for negative control (0.1% SDS), and 1 chick embryo for solvent control.
Comparative example 1
Comparative example 1 reference example 1 was prepared with the difference that: the raw materials adopted in the step (1) do not comprise fructus Tribuli and fructus Corni, but only comprise radix Sophorae Flavescentis; and does not include the fumigation treatment in step (1).
Comparative example 2
Comparative example 2 the preparation of reference example 1 is distinguished in that: the raw materials adopted in the step (1) do not comprise kuh-seng and dogwood fruits, but only comprise caltrop fruits; and does not include the fumigation treatment in step (1).
The color, appearance, smell and corresponding physicochemical index of the products obtained in comparative example 1 and comparative example 2 were recorded and tested, and the results are shown in table 9.
Table 9 results of product testing for different comparative examples
Experimental example 1
Human efficacy testing
The test method comprises the following steps:
before the capsaicin stimulates the face, 0min, 1min, 3min, 5min, 7min, 10min, 15min, 20min and 30min after the capsaicin stimulates the face, the TiVi 700 is used for collecting the images of the skin in the test area under polarized light, and the TiVi images are obtained through analysis. The Tivi 700 analysis image is characterized in that the stimulation degree is differentiated by colors through a digital method, and the more the colors are biased to red, the more the skin stimulation state is serious. The test results are shown in fig. 2 to 4.
The plant extract gel sample and blank matrix compositions of example 1 are shown in Table 10.
Table 10 gel sample composition
Remarks: wherein the amount of the plant extract in the plant extract gel sample is based on the total amount of the liquid plant extract product.
From the test results, compared with a blank matrix, the plant extract gel sample has obvious differences of 0min, 10min, 15min, 20min and 30min after use, and the plant extract gel has obvious red dispelling effect.
The red-removing effects of examples 23 to 26, example 33 and comparative examples 1 to 2 were further tested, and the plant extracts of each example and comparative example were respectively formulated into test samples according to the plant extract gel sample formulations in table 10, and tested according to the above-described test methods, and the test results are shown in fig. 5 and 6. The plant extracts of examples 23 to 26 and example 33 were found to have a less red-dispelling effect than the plant extract of example 1, as found from the skin color a value test results and the skin sensitivity Tivi-index test results.
Experimental example 2
Pi Fufan Red Signal Path test
1. Capsaicin stimulates keratinocytes (Ca 2+ Concentration of (C)
The calcium flux test method comprises the following steps:
(1) Cell inoculation: according to 1.8X10 5 Seeding Density of individual/well keratinocytes were seeded into 6-well plates, incubator (37 ℃, 5% CO) 2 ) Incubate overnight.
(2) Preparing liquid: and respectively preparing test object working solutions according to the test groups. Specific test packet information is shown in table 11. The specific solvent used to prepare the sample set was PBS buffer at a concentration of mass fraction based on the total mass of the plant extract of example 1.
Table 11 test packets
(3) Probe loading: cell culture medium was aspirated, washed 3 times with PBS, and each well was incubated with 0.5mL probe loading working solution in a 37℃incubator for 45min. After the incubation, the probe working solution was aspirated and washed 3 times with PBS.
(4) Administration: the BC group was added with 0.5mL of PBS per well, the NC group was added with PBS solution containing 0.05. Mu.M capsaicin CAP per well, the PC group was added with PBS solution containing 0.05. Mu.M capsaicin CAP, 3.9. Mu.g/mL trans-4-t-butylcyclohexanol, and the sample group was added with 0.5mL PBS solution containing 0.05. Mu.M capsaicin CAP, working concentration sample per well. After the end of the administration, the incubator incubates for 1h.
(5) And (3) calcium flow detection: after the incubation, the cell morphology was observed and photographed using a fluorescence microscope and collected at 200 x field of view. The photographing time is controlled at 30min.
(6) Results statistical analysis: graphPad Prism was used to map and the results were expressed as mean±sd. Comparisons between groups were performed using t-test statistical analysis. Statistical analysis was double tailed. P <0.05 was considered to have significant differences and P <0.01 was considered to have very significant differences.
The test results are shown in FIG. 7. As can be seen from FIG. 7, the plant extract of the present invention was effective in reducing Ca at 0.156%, 0.313% and 0.625% concentration 2+ And (3) ion inflow.
Experimental example 3
Inhibition of inflammatory mediator test
1. TNF-alpha (UVB-keratinocytes)
The testing method comprises the following steps: (1) cell seeding: according to 2.2X10 5 Seeding Density of individual/well keratinocytes were seeded into 6-well plates, incubator (37 ℃, 5% CO) 2 ) Incubate overnight.
(2) Preparing liquid: and respectively preparing test object working solutions according to the test groups. Specific test packet information is shown in table 12.
Table 12 test packet
Positive control group (dexamethasone) working solution preparation: mu.L of 10% mother liquor was taken up in 2mL of the culture solution to prepare 0.01% dexamethasone.
(3) Administration: according to the test scheme, when the cell plating rate in the 6-hole plate reaches 40% -60%, grouping drug administration is carried out, each hole is added with 2mL of sample, and each group is provided with 3 compound holes. After the completion of the administration, the 6-well plate was placed in an incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours.
(4) UVB irradiation: according to the test group, 300mJ/cm was performed on the group with UVB irradiation 2 Is irradiated with UVB.
(5) Post-incubation: after washing the cells with PBS, the 6-well plate was placed in an incubator (37 ℃ C., 5% CO) 2 ) Incubation was performed for 24h after the medium.
(6) ELISA detection: cell culture supernatants were collected and assayed according to the instructions of the ELISA assay kit.
The test results are shown in FIG. 8. As can be seen from FIG. 8, the plant extract of the present invention has an effect of inhibiting TNF-alpha expression at a concentration of 0.313% and 0.625%.
2. IL-8 (trending factor) (LPS-macrophage)
The testing method comprises the following steps:
(1) Cell inoculation: at 2.2X10 5 Cell was inoculated into 6-well plates, 2mL of cell suspension was added to each well, and the inoculated cell culture plates were placed in an incubator for continuous culture for 24 hours (5% CO) 2 、37℃)。
(2) Preparing liquid: and respectively preparing test object working solutions according to the test groups. Specific test packet information is shown in table 13.
Table 13 test packets
Positive control (dexamethasone) working solution preparation: mu.L of 10% mother liquor was taken up in 2mL of the culture solution to prepare 0.01% dexamethasone.
Preparation of working solution containing 1. Mu.g/mL LPS: 2mg/mL LPS stock solution was diluted to 1. Mu.g/mL with the test substance working solution, negative control working solution and positive control working solution remaining after the administration, respectively.
(3) Old cell culture fluid in the wells was aspirated. 1.8mL of culture solution containing the test substance with corresponding concentration is added into each hole of the sample group; 1.8mL of solvent control culture solution is added to each hole of the negative control group; adding 1.8mL of positive control culture solution into each hole of the positive control group; a blank control was added with 1.8mL of normal culture medium per well.
(4) After the completion of the administration, the 6-well plate was placed in a cell incubator (5% CO 2 Culturing at 37 ℃ for 2 hours.
(5) LPS stimulation: after 2h of administration, 200. Mu.L of the prepared working solution containing LPS was added to each well of each group, and the mixture was placed in a cell incubator (5% CO) 2 Culturing was continued at 37℃for 22 hours.
(6) ELISA detection: cell culture supernatants were collected and assayed by ELISA according to the ELISA kit instructions.
The test results are shown in FIG. 9. As is clear from FIG. 9, the plant extract of the present invention has the effect of inhibiting the expression of macrophage IL-8 and inhibiting the cascade amplification and angiogenesis of inflammation at a concentration of 0.625%.
Experimental example 4
Inhibition of vasodilator prostaglandin E2 (PGE-2), cyclooxygenase 2 (COX-2) (UVB-keratinocytes)
(1) PGE-2 assay
(1) Cell inoculation: according to 2.2X10 5 Seeding Density of individual/well keratinocytes were seeded into 6-well plates, incubator (37 ℃, 5% CO) 2 ) Incubate overnight.
(2) Preparing liquid: and respectively preparing test object working solutions according to the test groups. Specific test packet information is shown in table 14.
Table 14 test packets
Positive control group (dexamethasone) working solution preparation: mu.L of 10% mother liquor was taken up in 2mL of the culture solution to prepare 0.01% dexamethasone.
(3) Administration: according to the test scheme, when the cell plating rate in the 6-hole plate reaches 40% -60%, grouping drug administration is carried out, each hole is added with 2mL of sample, and each group is provided with 3 compound holes. After the completion of the administration, the 6-well plate was placed in an incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours.
(4) UVB irradiation: according to the test group, 300mJ/cm was performed on the group with UVB irradiation 2 Is irradiated with UVB.
(5) Post-incubation: after washing the cells with PBS, the 6-well plate was placed in an incubator (37 ℃ C., 5% CO) 2 ) Incubation was performed for 24h after the medium.
(6) ELISA detection: cell culture supernatants were collected and assayed according to the instructions of the ELISA assay kit.
The test results are shown in FIG. 10. As can be seen from fig. 10, the plant extracts of the present invention effectively reduced PGE2 release at concentrations of 0.156%, 0.313%, 0.625%.
(2) COX-2 assay
(1) Cell inoculation: according to 2.2X10 5 Seeding Density of individual/well keratinocytes were seeded into 6-well plates, incubator (37 ℃, 5% CO) 2 ) Incubate overnight.
(2) Preparing liquid: and respectively preparing test object working solutions according to the test groups. Specific test packet information is shown in table 15.
Table 15 test packets
Positive control group (dexamethasone) working solution preparation: mu.L of 10% mother liquor was taken up in 2mL of the culture solution to prepare 0.01% dexamethasone.
(3) Administration: according to the test scheme, the cell plating rate in the 6-hole plate reaches 40-60 percent% of the total amount was administered in groups of 2mL per well, and 3 wells were allocated per group. After the completion of the administration, the 6-well plate was placed in an incubator (37 ℃, 5% CO) 2 ) Is cultured for 24 hours.
(4) UVB irradiation: according to the test group, 300mJ/cm was performed on the group with UVB irradiation 2 Is irradiated with UVB.
(5) Post-incubation: after washing the cells with PBS, the 6-well plate was placed in an incubator (37 ℃ C., 5% CO) 2 ) Incubation was performed for 24h after the medium.
(6) And (3) gene expression detection: after 24h incubation, 1 mL/well PBS was washed twice, 1mL RNAiso Plus was added to each well, and after cell lysis was blown, the samples were collected. RNA was extracted, reverse transcribed to cDNA, and then subjected to fluorescent quantitative PCR detection, and the result was calculated by a 2-DeltaCT method.
The test results are shown in FIG. 11. As can be seen from FIG. 11, the plant extract of the present invention was effective in reducing COX-2 release at a concentration of 0.625%.
From the above test results, it is understood that the plant extract of the present invention can inhibit Ca 2+ The multi-dimensional effects of channel activation, inflammatory mediator inhibition, vasodilation factor inhibition and the like solve the problem of skin redness, and achieve the effect of rapidly dispelling the red.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (13)
1. The plant extract with the effect of removing red is characterized by comprising the following components in parts by mass:
1.5-6 parts of kuh-seng root extract, 1-4 parts of tribulus fruit extract, 0.5-2 parts of dogwood fruit extract, 85-95 parts of butanediol and 2-6 parts of water;
the kuh-seng root extract is ethanol water solution extract of kuh-seng root; the tribulus fruit extract is an ethanol water solution extract of tribulus fruit; the cornel fruit extract is ethanol water solution extract of cornel fruit;
in the ethanol water solution, the volume fraction of ethanol is 90% -95%;
before the radix sophorae flavescentis, the fructus tribuli and the fructus corni are extracted, fumigation treatment is carried out on the radix sophorae flavescentis, the fructus tribuli and the fructus corni.
2. The plant extract with the red dispelling effect according to claim 1, which is characterized by comprising the following components in parts by mass: 3 parts of kuh-seng root extract, 2 parts of tribulus fruit extract, 1 part of dogwood fruit extract, 90 parts of butanediol and 4 parts of water.
3. The method for preparing a plant extract with red dispelling effect according to any one of claims 1 to 2, characterized by comprising the following steps:
extracting radix sophorae flavescentis, fructus tribuli and fructus corni at 65-85 ℃ for 1-3 h by taking an ethanol water solution as an extraction solvent, wherein the feed-liquid ratio is 1:10-1:30;
concentrating the extracted liquid, and then redissolving by adopting 1, 3-butanediol.
4. The method for preparing a plant extract with red dispelling effect according to claim 3, further comprising: fumigating the kuh-seng, the tribulus fruit and the dogwood fruit before the extraction;
the fumigation treatment time is 20-40 min.
5. The method for producing a plant extract having a redness-removing effect according to claim 3, wherein the ratio of the feed liquid is 1:15 to 1:20;
and/or extracting at 75-85 ℃;
and/or extracting for 2-3 hours.
6. The method for preparing a plant extract with red dispelling effect according to claim 3, wherein the mass ratio of the kuh-seng, the fructus Tribuli and the fructus Corni is (15-60)/(10-40)/(5-20).
7. The method for preparing a plant extract with red dispelling effect according to claim 6, wherein the mass ratio of said kuh-seng, said fructus Tribuli and said fructus Corni is 3:2:1.
8. The method for producing a plant extract having a redness-removing effect according to claim 3, wherein the amount of 1, 3-butanediol used in the reconstitution is 10 to 25 times the weight of the crude drug based on the crude drug.
9. The method for preparing a plant extract with red dispelling effect according to claim 8, wherein the amount of 1, 3-butanediol is 10-16 times the weight of crude drug.
10. The method for preparing a plant extract with red dispelling effect according to claim 3, wherein said reconstitution comprises: 1, 3-butanediol was added in portions and concentrated after each addition.
11. The method for preparing a plant extract with red dispelling effect according to claim 10, wherein the 1, 3-butylene glycol is added in 3-5 batches.
12. The use of the plant extract with red dispelling effect of any one of claims 1 to 2 or the plant extract with red dispelling effect of any one of claims 3 to 11 in the preparation of skin care products.
13. The use according to claim 12, wherein the skin care product comprises a skin care product for sensitive muscles.
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