CN114041535A - Microbial preparation for improving intestinal environment of ruminants such as cattle and sheep and preparation method thereof - Google Patents
Microbial preparation for improving intestinal environment of ruminants such as cattle and sheep and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of microbial feed additives, and discloses a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep and a preparation method thereof, wherein the microbial preparation comprises 20-25 parts of wormwood, 20-25 parts of grape seeds, 10-15 parts of alfalfa, 10-15 parts of pharbitis seed, 8-10 parts of N-carbamylglutamic acid, 5-8 parts of mannan oligosaccharide, 5-8 parts of curvularin, 5-8 parts of vitamin C, 1-3 parts of nano kieselguhr and 6-8 parts of a composite microbial agent. The pure natural plant extract and the composite microbial inoculum have synergistic effect, so that the gastric emptying of ruminants such as cattle and sheep can be enhanced, the gastric motility disorder and abomasum displacement symptoms can be improved, the metabolic level can be improved, the dominant microbial community structure can be rapidly formed together with beneficial bacteria in the original intestinal tract, the growth of harmful bacteria in the intestinal tract can be inhibited, the micro-ecological environment of the intestinal tract of the ruminants such as cattle and sheep can be improved, and the immunity of the ruminants such as cattle and sheep can be improved.
Description
Technical Field
The invention belongs to the technical field of microbial feed additives, and particularly relates to a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep and a preparation method thereof.
Background
At present, the intestinal microbial ecosystem of animals is the biggest and most complex micro-ecosystem, and various bacteria are mutually restricted and interdependent. On one hand, the intestinal microorganisms can help the host digest food and provide various nutrients for the host; on the other hand, the micro-ecological balance in the intestinal tract of the host can be adjusted and maintained, and the micro-ecological balance has close relation with the growth, development, substance metabolism, aging and the like of the host, and plays an important role in the health of the host. The microbial preparation is a novel green feed additive developed in recent years, is non-toxic and residue-free, can regulate animal intestinal flora, inhibit harmful bacteria, promote growth and development, improve the ecological environment of livestock breeding, achieve the aim of ecological prevention and control, and has good economic benefit and ecological benefit.
In the prior art, related technologies for regulating the intestinal environment of ruminants such as cattle and sheep by simply relying on various probiotics exist, but the ruminants such as the cattle and the sheep are easy to cause diarrhea, gastric dyskinesia and abomasum displacement by simply relying on the probiotics, so that the effect is poor and the side effect is great.
Through the above analysis, the problems and defects of the prior art are as follows: in the prior art, the simple probiotics are used for regulating the intestinal environment of the ruminants such as cattle and sheep, so that the ruminants such as cattle and sheep are easy to cause diarrhea, gastric dyskinesia and abomasum displacement, and the regulation effect is poor and the side effect is great.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep and a preparation method thereof.
The microbial preparation for improving the intestinal environment of the ruminants such as the cattle and sheep consists of 20-25 parts of wormwood, 20-25 parts of grape seeds, 10-15 parts of alfalfa, 10-15 parts of pharbitis seed, 8-10 parts of N-carbamylglutamic acid, 5-8 parts of mannan oligosaccharide, 5-8 parts of curvularia polysaccharide, 5-8 parts of vitamin C, 1-3 parts of nano kieselguhr and 6-8 parts of composite microbial inoculum according to the mass parts.
Further, the composite microbial agent at least comprises any two of bacillus natto, bacillus subtilis, lactic acid bacteria, lactobacillus plantarum, enterococcus faecium and bacillus amyloliquefaciens.
The invention also aims to provide a preparation method of the microbial preparation for improving the intestinal environment of the ruminants such as cattle and sheep, wherein the preparation method of the microbial preparation for improving the intestinal environment of the ruminants such as cattle and sheep comprises the following steps:
weighing wormwood, grape seeds, alfalfa, pharbitis seed, N-carbamylglutamic acid, mannan oligosaccharide, yeast exopolysaccharide, vitamin C, nano kieselguhr and a composite microbial inoculum according to a proportion;
step two, preparing a wormwood extract by using the weighed wormwood; preparing resveratrol extract from grape seeds; preparing saponin extract from herba Medicaginis; preparing methanol extract from semen Pharbitidis;
uniformly mixing the wormwood extract, the resveratrol extract, the saponin extract and the methanol extract with mannan oligosaccharide, yeast exopolysaccharide, nano kieselguhr, a composite microbial inoculum and water, and then covering a preservative film for stacking and fermentation;
and step four, uniformly mixing the fermentation product with N-carbamylglutamic acid and vitamin C, and sterilizing by using a tunnel type sterilizing device to obtain the microbial preparation.
Further, the preparation of the wormwood extract by using the weighed wormwood comprises the following steps:
picking up the wormwood, and cleaning and drying the rhizome part of the wormwood after picking; crushing the dried wormwood rhizome part to obtain wormwood powder;
dissolving folium Artemisiae Argyi powder in water, and performing ultrasonic oscillation treatment; heating, distilling and filtering the solution after ultrasonic oscillation treatment to obtain essential oil and extraction residues;
adding water into the extraction residue again for heating and distilling, and repeating twice; and combining the essential oil obtained in the three times to obtain the wormwood essential oil.
Further, the preparation of the resveratrol extract by using the weighed grape seeds comprises the following steps:
washing grape seeds, dehydrating to obtain grape seeds with water content less than 8%, pulverizing the obtained grape seeds, and sieving with 100 mesh sieve to obtain grape seed powder;
adding a proper amount of ethanol into the grape seed powder, dissolving uniformly, and extracting in a constant-temperature water bath at 65 ℃ to obtain a first extracting solution and extracting residues;
extracting the grape seed extraction residues by using a supercritical extraction device; obtaining a second extracting solution and filter residue;
and combining the first extracting solution and the second extracting solution, concentrating under reduced pressure, drying and crushing to obtain the resveratrol extract.
Further, the extracting by using the supercritical extraction device comprises: at 40MPa, 70 ℃ and CO2The flow rate is 15kg/h, and extraction is carried out for 61 h.
Further, the preparation of the saponin extract by using alfalfa comprises the following steps:
cleaning alfalfa, naturally drying in the air, and crushing the alfalfa after drying in the air;
adding the crushed alfalfa into ethanol with the concentration of 60% to dissolve uniformly, placing the mixture into an ultrasonic device, and extracting for 1 hour at the temperature of 75 ℃ to obtain a primary extracting solution;
and adding ethanol into the residues obtained by ultrasonic extraction for reflux extraction to obtain a secondary extracting solution, concentrating the primary extracting solution and the secondary extracting solution until no alcohol exists, and performing spray drying to obtain the saponin extract.
Further, the preparation of the methanol extract by using the pharbitis seed comprises the following steps:
taking dried pharbitis seeds, and soaking the dried pharbitis seeds in a device filled with a methanol solution for 6 hours; heating the device filled with the pharbitis seed and the methanol solution in water bath to obtain an extracting solution;
and distilling the extracting solution under reduced pressure to recover methanol, and drying to constant weight to obtain a methanol extract.
Further, the stacking fermentation comprises: fermenting at 30 deg.C for 6 days; turning the pile once every 48 hours.
The invention also aims to provide application of the microbial preparation for improving the intestinal environment of the ruminants such as cattle and sheep in preparation of feed or feed additives for the ruminants such as cattle and sheep.
By combining all the technical schemes, the invention has the advantages and positive effects that: the pure natural plant extract and the composite microbial inoculum have synergistic effect, so that the gastric emptying of ruminants such as cattle and sheep can be enhanced, the gastric motility disorder and abomasum displacement symptoms can be improved, the metabolic level can be improved, the dominant microbial community structure can be rapidly formed together with beneficial bacteria in the original intestinal tract, the growth of harmful bacteria in the intestinal tract can be inhibited, the micro-ecological environment of the intestinal tract of the ruminants such as cattle and sheep can be improved, the immunity of the ruminants such as cattle and sheep can be improved, no residue exists in feed or additives, and the feed or additives have good economic benefit and ecological benefit.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a method for preparing a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep.
FIG. 2 is a flow chart of a method for preparing an Artemisia extract from a weighed Artemisia.
Fig. 3 is a flow chart of a preparation method of resveratrol extract from weighed grape seeds provided by the embodiment of the invention.
FIG. 4 is a flow chart of the method for preparing saponin extract using alfalfa according to embodiments of the present invention.
FIG. 5 is a flow chart of a method for preparing methanol extract from pharbitis seed according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep and a preparation method thereof, and the invention is described in detail below by combining the accompanying drawings.
The microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep and the like, provided by the embodiment of the invention, comprises, by mass, 20-25 parts of wormwood, 20-25 parts of grape seeds, 10-15 parts of alfalfa, 10-15 parts of pharbitis seeds, 8-10 parts of N-carbamylglutamic acid, 5-8 parts of mannan oligosaccharide, 5-8 parts of curvularin, 5-8 parts of vitamin C, 1-3 parts of nano diatomite and 6-8 parts of a composite microbial agent.
The composite microbial agent provided by the embodiment of the invention at least comprises any two of bacillus natto, bacillus subtilis, lactic acid bacteria, lactobacillus plantarum, enterococcus faecium and bacillus amyloliquefaciens.
As shown in fig. 1, a preparation method of a microbial preparation for improving intestinal environment of ruminants such as cattle and sheep provided by the embodiment of the invention comprises the following steps:
s101, weighing wormwood, grape seeds, alfalfa, pharbitis seed, N-carbamylglutamic acid, mannan oligosaccharide, yeast exopolysaccharide, vitamin C, nano kieselguhr and a composite microbial inoculum according to a proportion;
s102, preparing a wormwood extract by using the weighed wormwood; preparing resveratrol extract from grape seeds; preparing saponin extract from herba Medicaginis; preparing methanol extract from semen Pharbitidis;
s103, uniformly mixing the wormwood extract, the resveratrol extract, the saponin extract and the methanol extract with mannan oligosaccharide, yeast exopolysaccharide, nano kieselguhr, a composite microbial inoculum and water, and then covering a preservative film for stacking and fermentation;
s104, uniformly mixing the fermentation product with N-carbamylglutamic acid and vitamin C, and sterilizing by using a tunnel type sterilizing device to obtain the microbial preparation.
As shown in fig. 2, the preparation of the wormwood extract by using weighed wormwood according to the embodiment of the present invention includes:
s201, picking up the wormwood, and cleaning and drying the rhizome part of the wormwood after picking; crushing the dried wormwood rhizome part to obtain wormwood powder;
s202, dissolving wormwood powder in water and then carrying out ultrasonic oscillation treatment; heating, distilling and filtering the solution after ultrasonic oscillation treatment to obtain essential oil and extraction residues;
s203, adding water into the extraction residue again for heating distillation, and repeating twice; and combining the essential oil obtained in the three times to obtain the wormwood essential oil.
As shown in fig. 3, the preparation of the resveratrol extract by using weighed grape seeds provided by the embodiment of the present invention includes:
s301, washing grape seeds, dehydrating to obtain grape seeds with water content less than 8%, crushing the obtained grape seeds, and sieving with a 100-mesh sieve to obtain grape seed powder;
s302, adding a proper amount of ethanol into the grape seed powder, dissolving uniformly, and extracting in a constant-temperature water bath at 65 ℃ to obtain a first extracting solution and extracting residues;
s303, performing CO extraction on the grape seed extraction residues by using a supercritical extraction device at the temperature of 70 ℃ and 40MPa2Extracting for 61 hours at the flow rate of 15 kg/h; obtaining a second extracting solution and filter residue;
and S304, combining the first extracting solution and the second extracting solution, concentrating under reduced pressure, drying and crushing to obtain the resveratrol extract.
As shown in fig. 4, the preparation of saponin extract using alfalfa provided in the embodiments of the present invention includes:
s401, cleaning alfalfa, naturally drying, and crushing the alfalfa after air drying;
s402, adding the crushed alfalfa into ethanol with the concentration of 60% to dissolve uniformly, placing the mixture into an ultrasonic device, and extracting for 1 hour at 75 ℃ to obtain a primary extracting solution;
and S403, adding ethanol into the residues obtained by ultrasonic extraction for reflux extraction to obtain a secondary extracting solution, concentrating the primary extracting solution and the secondary extracting solution until no alcohol exists, and performing spray drying to obtain the saponin extract.
As shown in fig. 5, the preparation of methanol extract using pharbitis seed according to the embodiment of the present invention includes:
s501, taking dried pharbitis seeds, and soaking the dried pharbitis seeds in a device filled with a methanol solution for 6 hours; heating the device filled with the pharbitis seed and the methanol solution in water bath to obtain an extracting solution;
s502, distilling the extracting solution under reduced pressure to recover methanol, and drying to constant weight to obtain a methanol extract.
The stacking fermentation provided by the embodiment of the invention comprises the following steps: fermenting at 30 deg.C for 6 days; turning the pile once every 48 hours.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (10)
1. The microbial preparation for improving the intestinal environment of the ruminants such as the cattle and sheep is characterized by comprising, by mass, 20-25 parts of wormwood, 20-25 parts of grape seeds, 10-15 parts of alfalfa, 10-15 parts of pharbitis seeds, 8-10 parts of N-carbamylglutamic acid, 5-8 parts of mannan oligosaccharide, 5-8 parts of curvularia polysaccharide, 5-8 parts of vitamin C, 1-3 parts of nano kieselguhr and 6-8 parts of a composite microbial agent.
2. The microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep as claimed in claim 1, wherein the complex microbial preparation comprises at least two of Bacillus natto, Bacillus subtilis, lactic acid bacteria, Lactobacillus plantarum, enterococcus faecium, and Bacillus amyloliquefaciens.
3. A method for preparing the microbial preparation for improving the intestinal environment of the ruminants such as the cattle and the sheep, which is prepared by the microbial preparation for improving the intestinal environment of the ruminants such as the cattle and the sheep as claimed in claims 1-2, wherein the method for preparing the microbial preparation for improving the intestinal environment of the ruminants such as the cattle and the sheep comprises the following steps:
weighing wormwood, grape seeds, alfalfa, pharbitis seed, N-carbamylglutamic acid, mannan oligosaccharide, yeast exopolysaccharide, vitamin C, nano kieselguhr and a composite microbial inoculum according to a proportion;
step two, preparing a wormwood extract by using the weighed wormwood; preparing resveratrol extract from grape seeds; preparing saponin extract from herba Medicaginis; preparing methanol extract from semen Pharbitidis;
uniformly mixing the wormwood extract, the resveratrol extract, the saponin extract and the methanol extract with mannan oligosaccharide, yeast exopolysaccharide, nano kieselguhr, a composite microbial inoculum and water, and then covering a preservative film for stacking and fermentation;
and step four, uniformly mixing the fermentation product with N-carbamylglutamic acid and vitamin C, and sterilizing by using a tunnel type sterilizing device to obtain the microbial preparation.
4. The method for preparing a microbial preparation for improving the intestinal environment of a ruminant such as a cattle and sheep as claimed in claim 3, wherein the preparing of the mugwort extract using the weighed mugwort comprises:
picking up the wormwood, and cleaning and drying the rhizome part of the wormwood after picking; crushing the dried wormwood rhizome part to obtain wormwood powder;
dissolving folium Artemisiae Argyi powder in water, and performing ultrasonic oscillation treatment; heating, distilling and filtering the solution after ultrasonic oscillation treatment to obtain essential oil and extraction residues;
adding water into the extraction residue again for heating and distilling, and repeating twice; and combining the essential oil obtained in the three times to obtain the wormwood essential oil.
5. The method for preparing a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep as claimed in claim 3, wherein the step of preparing the resveratrol extract by using the weighed grape seeds comprises:
washing grape seeds, dehydrating to obtain grape seeds with water content less than 8%, pulverizing the obtained grape seeds, and sieving with 100 mesh sieve to obtain grape seed powder;
adding a proper amount of ethanol into the grape seed powder, dissolving uniformly, and extracting in a constant-temperature water bath at 65 ℃ to obtain a first extracting solution and extracting residues;
extracting the grape seed extraction residues by using a supercritical extraction device; obtaining a second extracting solution and filter residue;
and combining the first extracting solution and the second extracting solution, concentrating under reduced pressure, drying and crushing to obtain the resveratrol extract.
6. The method for preparing a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep as claimed in claim 5, wherein the extracting with the supercritical extraction apparatus comprises: at 40MPa, 70 ℃ and CO2Flow rate of 1At 5kg/h, extraction was carried out for 61 h.
7. The method for preparing a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep according to claim 3, wherein the preparation of the saponin extract using alfalfa comprises:
cleaning alfalfa, naturally drying in the air, and crushing the alfalfa after drying in the air;
adding the crushed alfalfa into ethanol with the concentration of 60% to dissolve uniformly, placing the mixture into an ultrasonic device, and extracting for 1 hour at the temperature of 75 ℃ to obtain a primary extracting solution;
and adding ethanol into the residues obtained by ultrasonic extraction for reflux extraction to obtain a secondary extracting solution, concentrating the primary extracting solution and the secondary extracting solution until no alcohol exists, and performing spray drying to obtain the saponin extract.
8. The method for preparing a microbial preparation for improving the intestinal environment of ruminants such as cattle and sheep according to claim 3, wherein the preparation of the methanol extract from pharbitis seed comprises:
taking dried pharbitis seeds, and soaking the dried pharbitis seeds in a device filled with a methanol solution for 6 hours; heating the device filled with the pharbitis seed and the methanol solution in water bath to obtain an extracting solution;
and distilling the extracting solution under reduced pressure to recover methanol, and drying to constant weight to obtain a methanol extract.
9. The method of claim 8, wherein the stacking fermentation comprises: fermenting at 30 deg.C for 6 days; turning the pile once every 48 hours.
10. Use of the microbial preparation according to any one of claims 1-2 for improving the intestinal environment of a ruminant such as cattle and sheep in the preparation of a feed or feed additive for a ruminant such as cattle and sheep.
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| CN115606698A (en) * | 2022-09-29 | 2023-01-17 | 贵州省畜牧兽医研究所 | Feed additive for preventing and treating digestive tract diseases of black goats and preparation method thereof |
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