CN102488089A - Preparation method of microecological culture feed additive - Google Patents
Preparation method of microecological culture feed additive Download PDFInfo
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- CN102488089A CN102488089A CN2011103664481A CN201110366448A CN102488089A CN 102488089 A CN102488089 A CN 102488089A CN 2011103664481 A CN2011103664481 A CN 2011103664481A CN 201110366448 A CN201110366448 A CN 201110366448A CN 102488089 A CN102488089 A CN 102488089A
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Abstract
A preparation method of a microecological culture feed additive belongs to the technical field of livestock and poultry feed additive. A solid fermentation medium comprises 40% of wheat bran, 10% of corn, 9% of soybean meal, 0.5% of urea, 0.1% of potassium dihydrogen phosphate, 0.1% of sodium dihydrogen phosphate, 0.1% of manganese sulfate, 0.2% of calcium chloride and 40% of water. fermentation strains include plant lactobacillus, bacillus subtilis and saccharomyces cerevisiae in a ratio of 1:2:2. The method comprises the following steps: crushing, sieving, mixing, disinfecting, preparing fermentation bacteria liquid, inoculating fermentation strains and obtaining a finished product. The microecological culture feed additive provided by the invention combines functions of nutrition and health care, has no toxic or side-effect or pollution, and can improve antioxidation capability and immunization function of a weaning pigling, increase daily weight gain of the pigling and forage conversion rate, reduce diarrhoea rate, reduce or prevent pigling weaning stress syndrome and substitute antibiotic additive in forage.
Description
Technical field
The invention belongs to the additive for farm animal feed technical field, be specifically related to a kind of preparation method of little ecological culture feed addictive.
Background technology
The early stage health of piglet is being played an important role aspect the raising piglet production performance.During weaned piglet; Since receive environment, nutrition, psychology etc. many-sided stress influence; The piglet digestive system development is not perfect in addition; Multiple digestive ferment merocrine secretion is not enough, usually shows phenomenons such as poor appetite, feed intake decline, indigestion, poor growth, efficiency of feed utilization are low, premunition decline, diarrhea rate, death rate increase, and so-called " stress of baby pigs caused ablaction syndrome " promptly takes place.The various stress reactions that produce behind the weaned piglet are a great problems of pig industry.And one of the most serious disease of the weanling pig especially of suffering from diarrhoea also is to cause one of most important reason that piglet is dead.The control of grice diarrhoea is a noticeable worldwide problem always; Prevention at present and treatment grice diarrhoea mainly adopt antibiotic; Though it has good effect to grice diarrhoea; But antibiotic prolonged application not only can cause resistance and strengthen that the piglet immunity of organisms descends, and can threaten to human beings'health because of residues of antibiotics.Therefore, seek antibiotic substitute that have no side effect, safe green, enjoyed industry to pay close attention in order to alleviate and to prevent this agricultural technology problem of diarrhea of weaned piglets.
Contain protein, peptide, amino acid, organic acid, oligosaccharides, nucleotides, ester class, alcohols, enzyme, beta glucan, manna oligosacchride, vitamin, mineral matter, nucleic acid, other UGF and beneficial microbe etc. in the microbial fermentation metabolite.Parasitic microbe in the piglet intestines and stomach can keep their metabolic activity and carry out normal metabolic under the nutrition substrate nourishing of little ecological culture stimulates; This will help safeguarding that the metabolic balance between each fungus strain also provides a good micro-ecological environment for the normal performance of pipe intestinal digesting absorption function; Make in the daily ration entrained various nutrients to be transported to more to absorb fully in the intestines and stomach and utilize, thereby satisfy piglet to greatest extent nutrient demand and the further performance that promotes its genetic potential.Therefore; The cooperative effect of multiple metabolic nutrition and non-nutritious matter can help piglet to set up and improve microecosystem in the intestines and stomach environment of unifying; Improve feed intake and improvement and digest and assimilate efficient; Thereby solve because functional disorder appears in metabolic dysequilibrium and the intestines and stomach of the parasitic bacteria that ablactation stress causes, realize prevention grice diarrhoea and the healthy growth that promotes them.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of little ecological culture, produce have run concurrently to support with health care be nontoxic, the side effect of one, the feed addictive of safe and effective prevention stress of baby pigs caused ablaction.
The present invention includes the following step:
1. solid-state fermentation culture medium and fermented bacterium preparation
1.1 solid-state fermentation culture medium is made up of by the quality proportioning following raw materials according: wheat bran 40%, corn 10%, dregs of beans 9%, urea 0.5%, potassium dihydrogen phosphate 0.1%, sodium dihydrogen phosphate 0.1%, manganese sulfate 0.1%, calcium chloride 0.2%, water 40%;
1.2 fermented bacterium is Lactobacillus plantarum, bacillus subtilis, the saccharomyces cerevisiae composite bacteria by 1: 2: 2 mixed, Lactobacillus plantarum wherein is a high yield CLA bacterium;
2. the concrete preparation of little ecological culture feed addictive comprises the following steps:
2.1 pulverize, sieve: the wheat bran in the solid-state fermentation culture medium, corn and dregs of beans are pulverized, crossed the 2.5mm mesh sieve;
2.2 mix, sterilize: the raw material in the solid-state fermentation culture medium is mixed,, be cooled to normal temperature and wait to inoculate through 115 ℃ of sterilizations of high-pressure steam sterilizing pan 30min;
2.3 zymocyte liquid preparation: the Lactobacillus plantarum of screening leaves standstill in 37 ℃ cultivates 48h, and bacillus subtilis is in 30 ℃ of shaken cultivation 48h, and saccharomyces cerevisiae leaves standstill in 28 ℃ cultivates 48h, makes the concentration of bacterium liquid all reach 10
8More than the cfu/ml;
2.4 inoculation fermentation bacterial classification: Lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae that step 2.3 obtains are pressed the bacterium liquid of 1: 2: 2 mixed, evenly be inoculated on the solid-state fermentation culture medium of step 2.2 acquisition, inoculum concentration is 3%;
2.5 fermentation: ventilate to stir, temperature is 28-30 ℃ condition bottom fermentation 24h, stop to ventilate intermittently stir, temperature is 33-35 ℃ condition bottom fermentation 48h;
2.6 finished product: tunning is a finished product in 45 ℃ of oven for drying.
The present invention utilizes raw materials such as corn, wheat bran, dregs of beans as fermentation medium; Through screen effective target composite bacteria; Carry out the little ecological culture feed addictive that inoculation fermentation forms; Can strengthen immunity and the antioxidative functional of piglet, improve its daily gain and efficiency of feed utilization, and stress of baby pigs caused ablaction syndrome is had prevention effects preferably.
The present invention is to provide a kind of run concurrently support with health care be the safe feed additive of one; Have no side effect, pollution-free, can improve the oxidation resistance and the immunologic function of weanling pig animal body; Can improve daily gain and the feed conversion rate of piglet; Reduce diarrhea rate, reduce or the syndromic generation of prevention stress of baby pigs caused ablaction the Antibiotic Additive in the also alternative feed.
The specific embodiment
For verifying effect of the present invention, carried out following test:
Adopt little ecological culture feed addictive provided by the invention, test period is 21 days.
The test packet design is selected (35 ± 1) age in days, average weight for use, and (10.06 ± 1.53kg) healthy ternarys (Du * length * big) are hybridized 60 of weanling pigs; Be divided into 4 processing at random; Each handles 3 repetitions, and each repeats 5, no significant difference (P>0.05) between the initial body weight between each repeating groups.
Table 1 experimental design
Diet is formed and the trophic level basal diet is prepared with reference to NRC piglet nutritional need, and basal diet is formed and trophic level is seen table 2.
A feeding and management test pig free choice feeding and drinking-water, routine immunization is responsible for feeding and management by the special messenger.The environmental condition unanimity is respectively organized in test.Every day the viewing test pig situation of only growing, search for food, record house temperature, diarrhoea situation and the dead number of washing in a pan.Duration of test diarrhoea piglet need not any antibiotic therapy.
Table 2 basal diet is formed and trophic level
Annotate: premix (every kilogram of diet content): vitamin A 1500IU; Cholecalciferol 3300IU; Vitamin E 60IU; Prokeyvit 6.0mg; Vitamin B1 3.0mg; Vitamin B2 9.0mg; Cobastab
66.0mg; Cobastab
120.03mg; Nicotinic acid 60.0mg; Pantothenic acid 18.0mg; Folic acid 1.5mg; Biotin 0.46mg; Choline Chloride 750mg; Iron 80mg; Copper 12mg; Zinc 75mg; Manganese 10mg; Iodine 0.30mg.
Crude protein, calcium and phosphorus are measured value, and other are calculated value.
The mensuration project
Test pig is weighed when the test starting and ending on an empty stomach, and the material of clearing residue simultaneously amount is measured average daily gain, average daily ingestion amount, feedstuff-meat ratio.
Diarrhea rate and diarrhoea index: every morning, piglet ight soil situation was observed in 8 timings, and a record diarrhoea number calculates diarrhea rate and diarrhoea index.
Diarrhea rate (%)=always number on the previous day/(total number * test fate) * 100% of suffering from diarrhoea
Diarrhoea index=stools scored sum/confession examination total number of pig (standards of grading are seen table 3)
Table 3 diarrhoea standards of grading
The data test data is carried out variance analysis with the SPSS12.0 statistical software to data after EXCEL handles, carry out multiple ratio with duncan's method, and result of the test is represented with average ± standard deviation.
1 little ecological culture feed addictive is seen table 4 to the influence of weanling pig production performance as a result.
The little ecological culture feed addictive of table 4 is to the influence of weanling pig production performance
Annotate: same column shoulder mark contains same letter person represents difference not significantly (P>0.05), shoulder mark different alphabetical person's significant differences (P<0.05), following table with.
Can know from last table, average daily gain test 1 group with other each group more all be significantly increased (P<0.05); Test 2 groups average daily gain and be significantly increased (P<0.05), and do not have notable difference (P>0.05) with the antibiotic control group than blank group; The antibiotic control group is than blank group be significantly improved (P<0.05).The average daily ingestion amount of test group is all than control group high (P<0.05), and between test group and the test group, indifference (P>0.05) then between control group and the control group.Feedstuff-meat ratio statistics between each group shows the trend same with daily gain.
2 little ecological culture feed addictives are seen table 5 to the influence of diarrhea of weaned piglets situation as a result.
The little ecological culture feed addictive of table 5 is to the influence of diarrhea of weaned piglets situation
Can know by last table; Testing 1 group, test 2 groups and antibiotic control group is reducing aspect the diarrhea of weaned piglets rate; All be significantly improved (P<0.05) than blank group, but test 1 group, the test 2 groups and and antibiotic control group three between do not have notable difference (P>0.05).The diarrhoea index also shows the trend same with diarrhea rate.
Conclusion is added little ecological culture feed addictive in the weanling pig basal diet; Can effectively alleviate the stress reaction that causes by various situation behind the weaned piglet; The speed of growth behind the raising weaned piglet, the feed intake that increases; Improve feedstuff-meat ratio and reduce diarrhea rate, and to draw the suitable addition of this little ecological culture feed addictive in the weanling pig diet be 0.25%.
Claims (2)
1. the preparation method of a little ecological culture feed addictive is characterized in that comprising the following steps:
1) solid-state fermentation culture medium and fermented bacterium preparation
1.1 solid-state fermentation culture medium is made up of by the quality proportioning following raw materials according: wheat bran 40%, corn 10%, dregs of beans 9%, urea 0.5%, potassium dihydrogen phosphate 0.1%, sodium dihydrogen phosphate 0.1%, manganese sulfate 0.1%, calcium chloride 0.2%, water 40%;
1.2 fermented bacterium is Lactobacillus plantarum, bacillus subtilis, the saccharomyces cerevisiae composite bacteria by 1: 2: 2 mixed;
2) the concrete preparation of little ecological culture feed addictive comprises the following steps:
2.1 pulverize, sieve: the wheat bran in the solid-state fermentation culture medium, corn and dregs of beans are pulverized, crossed the 2.5mm mesh sieve;
2.2 mix, sterilize: the raw material in the solid-state fermentation culture medium is mixed,, be cooled to normal temperature and wait to inoculate through 115 ℃ of sterilizations of high-pressure steam sterilizing pan 30min;
2.3 zymocyte liquid preparation: the Lactobacillus plantarum of screening leaves standstill in 37 ℃ cultivates 48h, and bacillus subtilis is in 30 ℃ of shaken cultivation 48h, and saccharomyces cerevisiae leaves standstill in 28 ℃ cultivates 48h, makes the concentration of bacterium liquid all reach 10
8More than the cfu/ml;
2.4 inoculation fermentation bacterial classification: Lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae that step 2.3 obtains are pressed the bacterium liquid of 1: 2: 2 mixed, evenly be inoculated on the solid-state fermentation culture medium of step 2.2 acquisition, inoculum concentration is 3%;
2.5 fermentation: ventilate to stir, temperature is 28-30 ℃ condition bottom fermentation 24h, stop to ventilate intermittently stir, temperature is 33-35 ℃ condition bottom fermentation 48h;
2.6 finished product: tunning is a finished product in 45 ℃ of oven for drying.
2. by the preparation method of the described little ecological culture feed addictive of claim 1, it is characterized in that the described Lactobacillus plantarum of step 1.2 is a high yield CLA bacterium.
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