CN104351570A - Compound Chinese herbal medicine probiotics for piglets and preparation method of compound Chinese herbal medicine probiotics - Google Patents
Compound Chinese herbal medicine probiotics for piglets and preparation method of compound Chinese herbal medicine probiotics Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a compound Chinese herbal medicine probiotics for piglets. The compound Chinese herbal medicine probiotics is prepared by feeding bacillus subtilis into extracting solutions of traditional Chinese medicines, and carrying out sealed anaerobic fermentation; the traditional Chinese medicines comprise the following components in parts by weight: 8-20 parts of radix astragali, 6-18 parts of dangshen, 8-16 parts of poria cocos, 1-15 parts of bighead atractylodes rhizome, 5-15 parts of white hyacinth beans, 5-10 parts of medicinal fermented mass, 5-15 parts of hawthorns, 1-10 parts of pericarpium citri reticulatae and 1-5 parts of liquorice. The compound Chinese herbal medicine probiotics is free from toxic and side effects and drug residue, and not only has the function of controlling diseases, but also is capable of providing multiple organic nutrient materials for animals, so that the growth and production performances of the animals can be improved.
Description
Technical field
The invention belongs to veterinary drug technical field, be specifically related to a kind of piglet compound Chinese herb probiotics and preparation method thereof.
Background technology
At present in order to prevent livestock and poultry, promote the utilization rate of animal growth and raising feed, the medicines such as a large amount of antibiotic, hormone are used as feed addictive.Although, the generation that the use of examples of such additives really effectively promotes the growth of livestock and poultry, improves feedstuff-meat ratio, decreases disease.But the side effect meanwhile brought also highlights day by day, such as long-term abuse of antibiotics causes drug-fast bacteria to produce in a large number, livestock meat egg dairy products drug residue increases, drug metabolism causes environmental pollution serious, and this series of problem directly threatens the health of the mankind.
Chinese herbal medicine and probio be current people generally acknowledge can be used for animal husbandry and have the green additive of remarkable result.Chinese herbal medicine belongs to natural drug, has no side effect, drug residue free, not only can play the effect of disease preventing and treating, and can improve multiple organic nutrient substance for animal, improves the growth production performance of animal.Probio can regulate GI colony balance, and then the generation improving immunity of organisms, promote growth of animal, reduce Animal diseases.Be applied in cultivation by the Chinese herbal medicine extract after probiotics fermention, have significant economic benefit and social benefit.
Summary of the invention
The object of the present invention is to provide a kind of piglet compound Chinese herb probiotics, this compound Chinese herb probiotics belongs to natural drug, have no side effect, more drug residue free, not only can play the effect of disease preventing and treating, and multiple organic nutrient substance can be improved for animal, improve the growth production performance of animal.
In order to achieve the above object, the present invention is by the following technical solutions:
The invention provides a kind of piglet compound Chinese herb probiotics, be add the fermentation of bacillus subtilis closing anaerobic by the extract of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: the Radix Astragali 8 ~ 20, Radix Codonopsis 6 ~ 18, Poria cocos 8 ~ 16, the bighead atractylodes rhizome 1 ~ 15, Semen Lablab Album 5 ~ 15, medicinal fermented mass 5 ~ 10, hawthorn 5 ~ 15, dried orange peel 1 ~ 10 and Radix Glycyrrhizae 1 ~ 5.
Described piglet compound Chinese herb probiotics, be add the fermentation of bacillus subtilis closing anaerobic by the extract of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: the Radix Astragali 10 ~ 15, Radix Codonopsis 9 ~ 15, Poria cocos 9 ~ 15, the bighead atractylodes rhizome 3 ~ 12, Semen Lablab Album 9 ~ 15, medicinal fermented mass 6 ~ 9, hawthorn 6 ~ 12, dried orange peel 3 ~ 9 and Radix Glycyrrhizae 1 ~ 3.
Described piglet compound Chinese herb probiotics, be add the fermentation of bacillus subtilis closing anaerobic by the extract of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: the Radix Astragali 15, Radix Codonopsis 10, Poria cocos 12, the bighead atractylodes rhizome 8, Semen Lablab Album 11, medicinal fermented mass 6, hawthorn 7, dried orange peel 5 and Radix Glycyrrhizae 2.
A piglet preparation method for compound Chinese herb probiotics, concrete steps are as follows:
(1) Chinese herbal medicine astragalus, Radix Codonopsis, Poria cocos, the bighead atractylodes rhizome, Semen Lablab Album, medicinal fermented mass, hawthorn, dried orange peel and Radix Glycyrrhizae being dried, pulverizing is that 40 ~ 80 order powder are for subsequent use;
(2) measure step (1) herb powder according to formula and add 2 times of water gaging Subcritical Water Extraction equipment effective component extractings, collect herb liquid, for subsequent use;
(3) get bacillus subtilis and carry out slant strains cultivation as bacterial classification, inclined-plane is cultivated the suspension produced anaerobism in the culture medium of correspondence and expand cultivation, to exponential phase clump count higher than 1.0 × 10
10during CFU/mL only, corresponding bacterium liquid is obtained;
(4) getting step (3) bacillus subtilis bacterium liquid by weight adds in step (2) gained herb liquid, weight ratio 1:(20 ~ 30 of bacillus subtilis bacterium liquid and herb liquid), anaerobic fermentation 24 ~ 36 hours under normal temperature, spraying dry, packing, obtains porcine compound Chinese herbal microecological preparation.
the present invention selects effect of raw material as follows:
The Radix Astragali: invigorating qi for strengthening superficies, diuresis holder poison, apocenosis, expelling pus and promoting granulation.Weak for the deficiency of vital energy, anorexia and loose stool, the sinking of qi of middle-jiao, rushes down prolapse of the anus etc. for a long time.Modern pharmacology proves that the coptis has enhanced machine body immunity function, promotes organism metabolism, cardiac stimulant, the effect of step-down.
Radix Codonopsis: Radix Codonopsis: tonifying middle-Jiao and Qi, invigorating the spleen benefit lung.Cure mainly the spleen deficiency syndrome of the lung weak, palpitaition of breathing hard, anorexia and loose stool etc.
Poria cocos: clearing damp and promoting diuresis, the peaceful heart of invigorating the spleen.Cure mainly oedema oliguria, phlegm and retained fluid is dizzy throbs with fear, and insufficiency of the spleen food is few, has loose bowels in loose stool.
The bighead atractylodes rhizome: strengthening the spleen and replenishing qi, eliminating dampness Li Shui, hidroschesis, antiabortive.Cure mainly insufficiency of the spleen food few, abdominal distension is had loose bowels etc.
Semen Lablab Album: invigorating the spleen for eliminating dampness, and in relieve summer heat.Cure mainly weakness of the spleen and the stomach, poor appetite, diarrhea.
Medicinal fermented mass: help digestion promoting the circulation of qi, invigorating the spleen to arrest diarrhea, induces sweat.Cure mainly dyspepsia not disappear, anorexia.
Hawthorn: food digesting stomach fortifying, promoting the circulation of qi scattered silt.Cure mainly carnivorous stagnant, gastral cavilty turgor, rushes down dysentery stomachache etc.
Dried orange peel: regulating qi-flowing for strengthening spleen is eliminating dampness and eliminating phlegm.Cure mainly chest gastral cavity turgor, food is few vomits and diarrhoea, coughing with a lot of sputum.
Radix Glycyrrhizae: invigorate the spleen and benefit qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, coordinating the drug actions of a prescription.
Bacillus subtilis: be vegetative bacteria.Self can secrete multiple digestive ferment and amino acid, promotes that animal is to the digestion of feed, simultaneously for animal body provides multiple nutrients material, thus promotes the growth of animal.By suppressing the harmful bacteria in animal body, keeping GI colony balance, can play and prevent and treat Animal diseases, the effect of Optimal culture environment.
compared with prior art, the beneficial effect that obtains of the present invention:
1. compound Chinese herb probiotics of the present invention belongs to natural drug, has no side effect, more drug residue free, not only can play the effect of disease preventing and treating, and can improve multiple organic nutrient substance for animal, improves the growth production performance of animal.
2. the present invention can adjust gastrointestinal bacterial flora balance by adding of bacillus subtilis, strengthens the immunity of body, reduces the generation of disease, growth promoting effects, improve livestock and poultry daily gain, improve the quality of livestock products.
3. what the present invention adopted Chinese herbal medicine and probio is combined with significant economic benefit and social benefit.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described, but do not limit content of the present invention.
embodiment 1
A kind of piglet compound Chinese herb probiotics, be add the fermentation of bacillus subtilis closing anaerobic by the extract of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: the Radix Astragali 8 ~ 20, Radix Codonopsis 6 ~ 18, Poria cocos 8 ~ 16, the bighead atractylodes rhizome 1 ~ 15, Semen Lablab Album 5 ~ 15, medicinal fermented mass 5 ~ 10, hawthorn 5 ~ 15, dried orange peel 1 ~ 10 and Radix Glycyrrhizae 1 ~ 5.
A piglet preparation method for compound Chinese herb probiotics, concrete steps are as follows:
(1) the raw material Radix Astragali, Radix Codonopsis, Poria cocos, the bighead atractylodes rhizome, Semen Lablab Album, medicinal fermented mass, hawthorn, dried orange peel and Radix Glycyrrhizae being dried, pulverizing is that 40 ~ 80 order powder are for subsequent use;
(2) measure step (1) herb powder according to formula and add 2 times of water gaging Subcritical Water Extraction equipment effective component extractings, collect herb liquid, for subsequent use;
(3) get bacillus subtilis and carry out slant strains cultivation as bacterial classification, inclined-plane is cultivated the suspension produced anaerobism in the culture medium of correspondence and expand cultivation, to exponential phase clump count higher than 1.0 × 10
10during CFU/mL only, corresponding bacterium liquid is obtained;
(4) getting step (3) bacillus subtilis bacterium liquid by weight adds in step (2) gained herb liquid, the weight ratio 1:25 of bacillus subtilis bacterium liquid and herb liquid, anaerobic fermentation 24 hours under normal temperature, spraying dry, packing, obtains porcine compound Chinese herbal microecological preparation.
embodiment 2
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 15, Radix Codonopsis 10, Poria cocos 12, the bighead atractylodes rhizome 8, Semen Lablab Album 11, medicinal fermented mass 6, hawthorn 7, dried orange peel 5 and Radix Glycyrrhizae 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 3
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 10, Radix Codonopsis 9, Poria cocos 9, the bighead atractylodes rhizome 3, Semen Lablab Album 9, medicinal fermented mass 6, hawthorn 6, dried orange peel 3 and Radix Glycyrrhizae 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 4
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 12, Radix Codonopsis 13, Poria cocos 11, the bighead atractylodes rhizome 10, Semen Lablab Album 12, medicinal fermented mass 8, hawthorn 10, dried orange peel 7 and Radix Glycyrrhizae 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 5
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 8, Radix Codonopsis 15, Poria cocos 12, the bighead atractylodes rhizome 15, Semen Lablab Album 5, medicinal fermented mass 10, hawthorn 6, dried orange peel 3 and Radix Glycyrrhizae 5.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 6
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 9, Radix Codonopsis 6, Poria cocos 8, the bighead atractylodes rhizome 10, Semen Lablab Album 14, medicinal fermented mass 5, hawthorn 12, dried orange peel 1 and Radix Glycyrrhizae 3.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 7
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 16, Radix Codonopsis 16, Poria cocos 9, the bighead atractylodes rhizome 1, Semen Lablab Album 5, medicinal fermented mass 9, hawthorn 6, dried orange peel 10 and Radix Glycyrrhizae 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 8
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 17, Radix Codonopsis 18, Poria cocos 10, the bighead atractylodes rhizome 4, Semen Lablab Album 7, medicinal fermented mass 8, hawthorn 5, dried orange peel 6 and Radix Glycyrrhizae 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 9
A kind of piglet compound Chinese herb probiotics, described Chinese medicine is made up of the component of following weight portion: the Radix Astragali 19, Radix Codonopsis 8, Poria cocos 11, the bighead atractylodes rhizome 6, Semen Lablab Album 12, medicinal fermented mass 6, hawthorn 12, dried orange peel 3 and Radix Glycyrrhizae 4.
The preparation method of the present embodiment is identical with embodiment 1.
With specific experiment, effect of the present invention is described below:
1 materials and methods:
1.1 trial targets: piglet of the present invention compound Chinese herb probiotics.Reference substance: Chinese herbal medicine granule
1.2 experimental animals: piglet.
1.3.1 experimental design
Each 120 of the weanling pig of 30 ages in days of Stochastic choice 10 plants.The product of the plant 1 spice feeding embodiment of the present invention 2, the product of the plant 2 spice feeding embodiment of the present invention 3, analogy successively, the product of plant 8 spice feeding example 9 of the present invention, plant 9 spice feeding Chinese herbal medicine granule, plant 10 feeds pure feed and does blank.Observe the clinical change of piglet every day, line item of going forward side by side, in table 1.
1.4 clinical test results
By table 1 statistical analysis, feed the daily gain of raising piglet in compound Chinese herb probiotics cultivation 1 ~ 8 group, be all greater than cultivation 9 and 10, feedstuff-meat ratio is little two groups later.In cultivation 1 ~ 8, the diarrhea disease percentage of piglet is all remarkable in cultivation 9 and cultivation 10(P < 0.01).Illustrate that piglet compound Chinese herb probiotics is by fermentation compared with simple Chinese herbal medicine granule, all have superiority in the growth performance of piglet and in control grice diarrhoea.
Claims (4)
1. a piglet compound Chinese herb probiotics, it is characterized in that: be add the fermentation of bacillus subtilis closing anaerobic by the extract of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: the Radix Astragali 8 ~ 20, Radix Codonopsis 6 ~ 18, Poria cocos 8 ~ 16, the bighead atractylodes rhizome 1 ~ 15, Semen Lablab Album 5 ~ 15, medicinal fermented mass 5 ~ 10, hawthorn 5 ~ 15, dried orange peel 1 ~ 10 and Radix Glycyrrhizae 1 ~ 5.
2. piglet compound Chinese herb probiotics according to claim 1, it is characterized in that: be add the fermentation of bacillus subtilis closing anaerobic by the extract of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: the Radix Astragali 10 ~ 15, Radix Codonopsis 9 ~ 15, Poria cocos 9 ~ 15, the bighead atractylodes rhizome 3 ~ 12, Semen Lablab Album 9 ~ 15, medicinal fermented mass 6 ~ 9, hawthorn 6 ~ 12, dried orange peel 3 ~ 9, Radix Glycyrrhizae 1 ~ 3 and bacillus subtilis 1 ~ 3.
3. piglet compound Chinese herb probiotics according to claim 1, it is characterized in that: be add the fermentation of bacillus subtilis closing anaerobic by the extract of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: the Radix Astragali 15, Radix Codonopsis 10, Poria cocos 12, the bighead atractylodes rhizome 8, Semen Lablab Album 11, medicinal fermented mass 6, hawthorn 7, dried orange peel 5, Radix Glycyrrhizae 2 and bacillus subtilis 2.
4. a piglet preparation method for compound Chinese herb probiotics as claimed in claim 1, it is characterized in that, concrete steps are as follows:
(1) Chinese herbal medicine astragalus, Radix Codonopsis, Poria cocos, the bighead atractylodes rhizome, Semen Lablab Album, medicinal fermented mass, hawthorn, dried orange peel and Radix Glycyrrhizae being dried, pulverizing is that 40 ~ 80 order powder are for subsequent use;
(2) measure step (1) herb powder according to formula and add 2 times of water gaging Subcritical Water Extraction equipment effective component extractings, collect herb liquid, for subsequent use;
(3) get bacillus subtilis and carry out slant strains cultivation as bacterial classification, inclined-plane is cultivated the suspension produced anaerobism in the culture medium of correspondence and expand cultivation, to exponential phase clump count higher than 1.0 × 10
10during CFU/mL only, corresponding bacterium liquid is obtained;
(4) getting step (3) bacillus subtilis bacterium liquid by weight to add in step (2) gained in drug filtrate, weight ratio 1:(20 ~ 30 of bacillus subtilis bacterium liquid and herb liquid), anaerobic fermentation 24 ~ 36 hours under normal temperature, spraying dry, packing, obtains porcine compound Chinese herbal microecological preparation.
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