CN115606698A - Feed additive for preventing and treating digestive tract diseases of black goats and preparation method thereof - Google Patents

Feed additive for preventing and treating digestive tract diseases of black goats and preparation method thereof Download PDF

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Publication number
CN115606698A
CN115606698A CN202211202589.4A CN202211202589A CN115606698A CN 115606698 A CN115606698 A CN 115606698A CN 202211202589 A CN202211202589 A CN 202211202589A CN 115606698 A CN115606698 A CN 115606698A
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extract
parts
digestive tract
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treating
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杨莉
徐景峨
蒲龄
李婷
李美娟
张亚楠
杨粤黔
颜永群
黄开荣
陈晓岚
单鲁先
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Guizhou Institute Of Animal Husbandry And Veterinary Medicine
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14Pretreatment of feeding-stuffs with enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/175Rhamnosus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/41Pediococcus
    • A23V2400/413Acidilactici

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Abstract

The invention belongs to the technical field of preparation of feed additives, and discloses a feed additive for preventing and treating digestive tract diseases of black goats and a preparation method thereof, wherein the feed additive for preventing and treating the digestive tract diseases of the black goats comprises, by mass, 20-25 parts of poplar leaves, 20-25 parts of stylosanthes guianensis, 10-15 parts of piper sarmentosum, 10-15 parts of schizonepeta, 10-15 parts of cattail grass, 10-15 parts of pogostemon cablin, 5-8 parts of gynostemma pentaphylla, 5-8 parts of protein powder and 5-8 parts of a microbial agent. The microbial agent is one or more of lactobacillus plantarum, bacillus subtilis, lactobacillus rhamnosus and pediococcus acidilactici. The invention can promote the digestive ability of the digestive system of the black goat intestines and stomach, enhance the digestion and absorption of feed and other foods, regulate and control the flora of the digestive tract, protect the intestines and stomach functions of the black goat and promote the healthy growth of the black goat.

Description

Feed additive for preventing and treating digestive tract diseases of black goats and preparation method thereof
Technical Field
The invention belongs to the technical field of feed additive preparation, and particularly relates to a feed additive for preventing and treating digestive tract diseases of black goats and a preparation method thereof.
Background
At present, the black goat has the advantages of fine muscle fiber, small hardness, tender meat, delicious taste, little mutton smell, high nutritional value, more than 22.6 percent of protein content, less than 3 percent of fat content, lower cholesterol content, 75 percent lower than pork, 62 percent lower than beef and sheep mutton, more than 15 essential amino acids for human body, particularly high glutamic acid content which is up to 11.03 percent, has the effects of nourishing yin and strengthening yang, tonifying deficiency and strengthening body, improving human immunity, prolonging life and beautifying, particularly has obvious nourishing effect on old and weak and sick patients, and is suitable for both the old and the young.
However, the black goat often suffers from digestive tract diseases and is easy to have symptoms such as inappetence, diarrhea and the like, the prior art mainly treats the black goat by using drugs such as antibiotics and the like after the relevant symptoms appear, the treatment effect is general and the prevention effect cannot be achieved, nonpathogenic sensitive flora in the gastrointestinal tract of the black goat is inhibited, and the fatal drug-resistant flora is propagated in large quantities by a machine, so that the diseases such as enteritis, pneumonia and the like are caused, and even acidosis is caused. In addition, because antibacterial drugs interfere with the normal flora of the digestive tract, vitamin B or K deficiency can be caused, inducing superinfection.
Through the above analysis, the problems and defects of the prior art are as follows: the prior art does not have a feed additive capable of preventing and treating digestive tract diseases of the black goats, and the existing black goat digestive tract disease treatment drugs or methods have common treatment effects, cannot achieve the prevention effect and are not beneficial to the health of the black goats.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a feed additive for preventing and treating digestive tract diseases of black goats and a preparation method thereof.
The invention is realized in such a way that the preparation method of the feed additive for preventing and treating the digestive tract diseases of the black goats comprises the following steps:
step one, obtaining poplar leaves without disease spots and insect changes, and weighing the poplar leaves without disease spots and insect changes, stylosanthes guianensis, sarmentosa, schizonepeta, cattail grass, pogostemon cablin, gynostemma pentaphylla, protein powder and microbial agent in proportion;
screening the weighed poplar leaves, removing impurities or miscellaneous leaves, cleaning and drying by using a dryer; crushing the dried poplar leaves by using a crusher, and sieving the crushed poplar leaves by using a 80-120-mesh sieve to obtain poplar leaf powder;
step three, cleaning, airing, crushing, repeatedly leaching with propylene glycol, filtering and concentrating the obtained sarmentosum to obtain a sarmentosum extract; drying the weighed stylosanthes guianensis, performing microwave treatment and carbon dioxide supercritical extraction to obtain a stylosanthes guianensis extract;
step four, decocting the weighed radix ranunculi ternati for multiple times, combining the decoctions, and filtering to obtain a radix ranunculi ternati extracting solution; carrying out wet grinding, enzymolysis, leaching and filtering on the weighed gynostemma pentaphylla to obtain a gynostemma pentaphylla extracting solution; respectively pretreating schizonepeta and pogostemon cablin to obtain pretreated schizonepeta and pogostemon cablin;
and step five, uniformly stirring and mixing protein powder, a microbial agent, poplar leaf powder, a sarmentosum bunge extract, a stylosanthes guianensis extract, a cattail extract, a gynostemma pentaphylla extract, the pretreated schizonepeta and the pogostemon cablin by using a stirrer, carrying out superfine grinding, and carrying out sterilization to obtain the feed additive for preventing and treating the digestive tract diseases of the black goats.
Further, in the second step, the drying by the dryer includes:
determining the current water content of the poplar leaves, and determining the expected water content of the poplar leaves to be 10%; determining a drying temperature and time of a dryer based on a difference between a current moisture content and a desired moisture content of the poplar leaves;
controlling the dryer to preheat, and controlling the preheated dryer to execute drying processing based on the determined drying temperature and time of the dryer; detecting whether the working parameters of the dryer are matched with the determined drying temperature direction in real time; if not, adjusting the working parameters of the dryer;
judging whether the drying time reaches the determined time or not, and if so, taking out the poplar leaves; otherwise, continuing the drying treatment until the drying time reaches the determined time.
Further, in the third step, the obtained sarmentosum bungei is washed, dried, crushed, extracted with propylene glycol for multiple times, filtered and concentrated to obtain the sarmentosum bungei extract, which comprises the following steps:
(1) Washing the weighed sarmentosum bunge root with deionized water for 3 minutes, airing the sarmentosum bunge root in a shade place, and carrying out high-pressure treatment on the aired sarmentosum bunge root;
(2) Crushing the high-pressure treated sarmentosum bunge root by using a crushing device, and sieving to obtain sarmentosum bunge root powder;
(3) Adding 8 times of propylene glycol into the obtained sarmentosum bunge root powder, and leaching for 48-56 hours at the temperature of 30-35 ℃ in a water bath environment;
(4) Repeating the step (3) for 2-3 times, filtering and combining the sarmentosum bunge extract, and concentrating the obtained sarmentosum bunge extract to obtain the sarmentosum bunge extract.
Further, in the third step, the air-drying, microwave treatment and carbon dioxide supercritical extraction are carried out on the weighed stylosanthes guianensis to obtain the stylosanthes guianensis extract, wherein the step of preparing the stylosanthes guianensis extract comprises the following steps:
(1) Removing seeds and impurities from the weighed stylosanthes guianensis, drying in the sun in the burning sun, adding ethanol into the dried stylosanthes guianensis, and performing microwave treatment at 80-100W for 2-3 hours to obtain a treated mixed solution;
(2) Centrifuging the treated mixed solution by using a centrifugal machine, and filtering to obtain a primary stylosanthes guianensis extracting solution and stylosanthes guianensis residues;
(3) Performing carbon dioxide supercritical extraction on the stylosanthes guianensis residue to obtain a stylosanthes guianensis secondary extracting solution; and uniformly mixing the primary extract of the stylosanthes guianensis and the secondary extract of the stylosanthes guianensis, and filtering by using an ultrafiltration membrane to obtain the stylosanthes guianensis extract.
Further, in the fourth step, decocting the weighed radix catnip for multiple times, mixing the decoctions, and filtering to obtain an extract of the radix catnip comprises:
(1) Cleaning and airing the felwort, and cutting the aired felwort into sections to obtain felwort sections;
(2) Adding 10 times of deionized water into the obtained radix Ranunculi Ternati section, soaking for 1 hr, and decocting the soaked radix Ranunculi Ternati and soaking water in a Chinese medicinal decocting pot;
(3) And combining the obtained decoctions, and performing multiple filtration and impurity removal by using a filter device to obtain the radix ranunculi ternati extract.
Further, the decocting comprises: decocting for 3-4 hr for 2-3 times.
Further, in the fourth step, the step of wet grinding, enzymolysis, leaching and filtering the weighed gynostemma pentaphylla to obtain a gynostemma pentaphylla extracting solution comprises the following steps:
(1) Adding a certain amount of clear water into gynostemma pentaphylla, and carrying out wet grinding by using a wet grinding device to obtain gynostemma pentaphylla slurry;
(2) Adding cellulase into the obtained herba Gynostemmatis slurry, and performing enzymolysis at normal temperature for 40-45min to obtain herba Gynostemmatis enzymolysis liquid and herba Gynostemmatis residue;
(3) Adding a mixed solvent obtained by mixing propylene glycol and dichloromethane according to a ratio of 1;
(4) Mixing the Gynostemma pentaphylla enzymolysis liquid and the Gynostemma pentaphylla extracting solution, and filtering by using an ultrafiltration membrane to obtain the Gynostemma pentaphylla extracting solution.
The invention also aims to provide the feed additive for preventing and treating the digestive tract diseases of the black goats, which is prepared by the preparation method of the feed additive for preventing and treating the digestive tract diseases of the black goats, and the feed additive for preventing and treating the digestive tract diseases of the black goats consists of 20-25 parts of poplar leaves, 20-25 parts of stylosanthes guianensis, 10-15 parts of sarcandra glabra, 10-15 parts of schizonepeta, 10-15 parts of radix ranunculi ternati, 10-15 parts of cablin potchouli herb, 5-8 parts of gynostemma pentaphylla, 5-8 parts of protein powder and 5-8 parts of microbial agent in parts by mass.
Further, the microbial agent is one or more of lactobacillus plantarum, bacillus subtilis, lactobacillus rhamnosus and pediococcus acidilactici.
The invention also aims to provide the feed for preventing and treating the digestive tract diseases of the black goats, and the feed additive for preventing and treating the digestive tract diseases of the black goats is added into the feed for preventing and treating the digestive tract diseases of the black goats.
In combination with the technical solutions and the technical problems to be solved, please analyze the advantages and positive effects of the technical solutions to be protected in the present invention from the following aspects:
first, aiming at the technical problems existing in the prior art and the difficulty in solving the problems, the technical problems to be solved by the technical scheme of the present invention are closely combined with the technical scheme to be protected and the results and data in the research and development process, and some creative technical effects brought after the problems are solved are analyzed in detail and deeply. The specific description is as follows:
the feed additive can promote the digestive ability of the gastrointestinal digestive system of the black goat, enhance the digestion and absorption of the feed and other foods, regulate and control the flora in the digestive tract, protect the gastrointestinal function of the black goat and promote the healthy growth of the black goat.
Secondly, considering the technical scheme as a whole or from the perspective of products, the technical effect and advantages of the technical scheme to be protected by the invention are specifically described as follows:
the feed additive for preventing and treating the digestive tract diseases of the black goats, disclosed by the invention, is added into the daily ration of the black goats, so that the digestive tract diseases of the black goats can be effectively prevented and treated, the symptoms such as diarrhea and the like are avoided, and the black goats are promoted to better and more quickly absorb nutrient substances in the daily ration.
Drawings
FIG. 1 is a flow chart of a preparation method of a feed additive for preventing and treating digestive tract diseases of a black goat provided by the embodiment of the invention;
fig. 2 is a flow chart of the method for washing, drying, pulverizing, leaching with propylene glycol for multiple times, filtering, and concentrating the obtained sarmentose pepper to obtain sarmentose pepper extract according to the embodiment of the present invention;
fig. 3 is a flow chart of a method for obtaining a pillar flower and grass extract by airing, microwave treatment and carbon dioxide supercritical extraction of the weighed pillar flower and grass.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
1. Illustrative embodiments are explained. This section is an illustrative example developed to explain the claims in order to enable those skilled in the art to fully understand how to implement the present invention.
The feed additive for preventing and treating the digestive tract disease of the black goat, provided by the embodiment of the invention, is characterized by comprising 20-25 parts of poplar leaves, 20-25 parts of stylosanthes guianensis, 10-15 parts of sarmentosum, 10-15 parts of schizonepeta, 10-15 parts of felwort, 10-15 parts of pogostemon cablin, 5-8 parts of gynostemma pentaphylla, 5-8 parts of protein powder and 5-8 parts of microbial agent in parts by mass.
The microbial agent provided by the embodiment of the invention is one or more of lactobacillus plantarum, bacillus subtilis, lactobacillus rhamnosus and pediococcus acidilactici.
As shown in fig. 1, the preparation method of the feed additive for preventing and treating digestive tract diseases of black goats, provided by the embodiment of the present invention, comprises:
s101, obtaining poplar leaves free of disease spots and insect changes, and weighing the poplar leaves free of disease spots and insect changes, stylosanthes guianensis, sarmentosa, sarmentosum, schizonepeta, cattail grass, pogostemon cablin, gynostemma pentaphylla, protein powder and microbial agent in proportion;
s102, screening the weighed poplar leaves, removing impurities or miscellaneous leaves, cleaning and drying by using a dryer; crushing the dried poplar leaves by using a crusher, and sieving the crushed poplar leaves by using a 80-120-mesh sieve to obtain poplar leaf powder;
s103, cleaning, airing, crushing, repeatedly leaching with propylene glycol, filtering and concentrating the obtained sarmentosum bungei to obtain sarmentosum bungei extract; drying the weighed stylosanthes guianensis, performing microwave treatment and carbon dioxide supercritical extraction to obtain a stylosanthes guianensis extract;
s104, decocting the weighed radix ranunculi ternati for multiple times, combining decoctions, and filtering to obtain a radix ranunculi ternati extracting solution; carrying out wet grinding, enzymolysis, leaching and filtering on the weighed gynostemma pentaphylla to obtain a gynostemma pentaphylla extracting solution; respectively pretreating schizonepeta and pogostemon cablin to obtain pretreated schizonepeta and pogostemon cablin;
and S105, uniformly stirring and mixing protein powder, a microbial agent, poplar leaf powder, a sarmentosum bunge extract, a stylosanthes guianensis extract, a cattail extract, a gynostemma pentaphylla extract, the pretreated schizonepeta and pogostemon cablin by using a stirrer, carrying out superfine grinding, and sterilizing to obtain the feed additive for preventing and treating the digestive tract diseases of the black goats.
In step S102, the drying with the dryer according to the embodiment of the present invention includes:
determining the current moisture content of the poplar leaves, and determining the expected moisture content of the poplar leaves to be 10%; determining a drying temperature and time of a dryer based on a difference between a current moisture content and a desired moisture content of the poplar leaves;
controlling the dryer to preheat, and controlling the preheated dryer to execute drying processing based on the determined drying temperature and time of the dryer; detecting whether the working parameters of the dryer are matched with the determined drying temperature direction in real time; if not, adjusting the working parameters of the dryer;
judging whether the drying time reaches the determined time or not, and if so, taking out the poplar leaves; otherwise, continuing the drying treatment until the drying time reaches the determined time.
As shown in fig. 2, the washing, air drying, crushing, multiple times of propylene glycol leaching, filtering and concentrating of the obtained sarmentose pepper according to the embodiment of the present invention to obtain the sarmentose pepper extract includes:
s201, washing the weighed sarmentosum bunge root for 3 minutes by using deionized water, airing the sarmentosum bunge root in a shade place, and carrying out high-pressure treatment on the aired sarmentosum bunge root;
s202, crushing the high-pressure treated sarmentosum bunge root by using a crushing device, and sieving to obtain sarmentosum bunge root powder;
s203, adding 8 times of propylene glycol into the obtained sarmentosum bunge root powder, and leaching for 48-56 hours at the temperature of 30-35 ℃ in a water bath environment;
s204, repeating the step S203 for 2-3 times, filtering and combining the sarmentosum bunge extract, and concentrating the obtained sarmentosum bunge extract to obtain the sarmentosum bunge extract.
As shown in fig. 3, the drying, microwave treatment and carbon dioxide supercritical extraction of the weighed stylosanthes guianensis provided by the embodiment of the present invention to obtain the stylosanthes guianensis extract includes:
s301, removing seeds and impurities from the weighed stylosanthes guianensis, drying in the sun in the burning sun, adding ethanol into the dried stylosanthes guianensis, and performing microwave treatment at 80-100W for 2-3 hours to obtain a treated mixed solution;
s302, centrifuging the treated mixed solution by using a centrifuge, and filtering to obtain a primary stylosanthes guianensis extracting solution and stylosanthes guianensis residues;
s303, performing carbon dioxide supercritical extraction on the stylosanthes guianensis residues to obtain a stylosanthes guianensis secondary extracting solution; and uniformly mixing the primary extract of the stylosanthes guianensis and the secondary extract of the stylosanthes guianensis, and filtering by using an ultrafiltration membrane to obtain the stylosanthes guianensis extract.
The method for decocting the weighed radix ranunculi ternati for multiple times, combining the decoctions and filtering to obtain the radix ranunculi ternati extracting solution comprises the following steps:
(1) Cleaning and airing the cattail grass, and cutting the aired cattail grass into sections to obtain a cattail grass section;
(2) Soaking the obtained radix Ranunculi Ternati section in 10 times of deionized water for 1 hr, and decocting the soaked radix Ranunculi Ternati and soaking water in a Chinese medicinal decocting pot for 2-3 times, each time for 3-4 hr;
(3) And combining the obtained decoctions, and performing multiple filtration and impurity removal by using a filter device to obtain the radix ranunculi ternati extract.
The method for obtaining the gynostemma pentaphylla extract by wet grinding, enzymolysis, leaching and filtering the weighed gynostemma pentaphylla provided by the embodiment of the invention comprises the following steps:
firstly, adding a certain amount of clear water into gynostemma pentaphylla, and carrying out wet grinding by using a wet grinding device to obtain gynostemma pentaphylla slurry;
secondly, adding cellulase into the obtained gynostemma pentaphylla slurry, and carrying out enzymolysis for 40-45min at normal temperature to obtain gynostemma pentaphylla enzymolysis liquid and gynostemma pentaphylla residues;
then, carrying out oscillating extraction on the gynostemma pentaphylla residues with a mixed solvent on a shaking table for 10-20 hours, centrifuging and filtering to obtain a gynostemma pentaphylla extracting solution;
and finally, mixing the gynostemma pentaphylla enzymolysis liquid and the gynostemma pentaphylla extracting solution, and filtering by using an ultrafiltration membrane to obtain the gynostemma pentaphylla extracting solution.
The mixed solvent provided by the embodiment of the invention is prepared by mixing propylene glycol and dichloromethane according to the proportion of 1.
2. Application examples. In order to prove the creativity and the technical value of the technical scheme of the invention, the part is an application example of the technical scheme of the claims to a specific product or related technology.
The feed additive for preventing and treating the digestive tract diseases of the black goats, provided by the embodiment of the invention, is added into the daily ration of the black goats, so that the diarrhea of the black goats can be effectively prevented and treated, and the black goats are promoted to better and more quickly absorb the nutrients in the daily ration.
The above description is only for the purpose of illustrating the embodiments of the present invention, and the scope of the present invention should not be limited thereto, and any modifications, equivalents and improvements made by those skilled in the art within the technical scope of the present invention as disclosed in the present invention should be covered by the scope of the present invention.

Claims (10)

1. A preparation method of a feed additive for preventing and treating digestive tract diseases of black goats is characterized by comprising the following steps:
step one, obtaining poplar leaves without disease spots and insect changes, and weighing the poplar leaves without disease spots and insect changes, stylosanthes guianensis, sarmentosa, schizonepeta, cattail grass, pogostemon cablin, gynostemma pentaphylla, protein powder and microbial agent in proportion;
screening the weighed poplar leaves, removing impurities or miscellaneous leaves, cleaning and drying by using a dryer; crushing the dried poplar leaves by using a crusher, and sieving the crushed poplar leaves by using a 80-120-mesh sieve to obtain poplar leaf powder;
step three, cleaning, airing, crushing, repeatedly leaching with propylene glycol, filtering and concentrating the obtained sarmentosum to obtain a sarmentosum extract; drying the weighed stylosanthes guianensis, performing microwave treatment and carbon dioxide supercritical extraction to obtain a stylosanthes guianensis extract;
step four, decocting the weighed radix ranunculi ternati for multiple times, combining the decoctions, and filtering to obtain a radix ranunculi ternati extracting solution; carrying out wet grinding, enzymolysis, leaching and filtering on the weighed gynostemma pentaphylla to obtain a gynostemma pentaphylla extracting solution; respectively pretreating schizonepeta and pogostemon cablin to obtain pretreated schizonepeta and pogostemon cablin;
and step five, uniformly stirring and mixing protein powder, a microbial agent, poplar leaf powder, a sarmentosum bunge extract, a stylosanthes guianensis extract, a cattail extract, a gynostemma pentaphylla extract, the pretreated schizonepeta and the pogostemon cablin by using a stirrer, carrying out superfine grinding, and carrying out sterilization to obtain the feed additive for preventing and treating the digestive tract diseases of the black goats.
2. The method for preparing the feed additive for preventing and treating the digestive tract disease of the black goat as claimed in claim 1, wherein the step two of drying by using the dryer comprises:
determining the current moisture content of the poplar leaves, and determining the expected moisture content of the poplar leaves to be 10%; determining a drying temperature and time of a dryer based on a difference between a current moisture content and a desired moisture content of the poplar leaves;
controlling the dryer to preheat, and controlling the preheated dryer to execute drying processing based on the determined drying temperature and time of the dryer; detecting whether the working parameters of the dryer are matched with the determined drying temperature direction in real time; if not, adjusting the working parameters of the dryer;
judging whether the drying time reaches the determined time or not, and if so, taking out the poplar leaves; otherwise, continuing the drying treatment until the drying time reaches the determined time.
3. The method for preparing the feed additive for preventing and treating the digestive tract diseases of the black goats according to claim 1, wherein the step three comprises the following steps of washing, airing, crushing, extracting with propylene glycol for multiple times, filtering and concentrating the obtained sarmentosum to obtain the sarmentosum extract:
(1) Washing the weighed sarmentosum bunge root with deionized water for 3 minutes, airing the sarmentosum bunge root in a shade place, and carrying out high-pressure treatment on the aired sarmentosum bunge root;
(2) Pulverizing the high-pressure treated sarmentose pepper root by a pulverizing device, and sieving to obtain sarmentose pepper root powder;
(3) Adding 8 times of propylene glycol into the obtained sarmentosum bungei root powder, and leaching for 48-56 hours at the temperature of 30-35 ℃ in a water bath environment;
(4) Repeating the step (3) for 2-3 times, filtering and combining the sarmentosum bunge extract, and concentrating the obtained sarmentosum bunge extract to obtain the sarmentosum bunge extract.
4. The preparation method of the feed additive for preventing and treating digestive tract diseases of black goats according to claim 1, wherein in the third step, the air-drying, microwave treatment and carbon dioxide supercritical extraction of the weighed stylosanthes guianensis are carried out to obtain the stylosanthes guianensis extract, and the preparation method comprises the following steps:
(1) Removing seeds and impurities from the weighed stylosanthes guianensis, drying in the sun in the burning sun, adding ethanol into the dried stylosanthes guianensis, and performing microwave treatment at 80-100W for 2-3 hours to obtain a treated mixed solution;
(2) Centrifuging the treated mixed solution by using a centrifugal machine, and filtering to obtain a primary stylosanthes guianensis extracting solution and stylosanthes guianensis residues;
(3) Performing carbon dioxide supercritical extraction on the stylosanthes guianensis residues to obtain a stylosanthes guianensis secondary extracting solution; and uniformly mixing the primary extract of the stylosanthes guianensis and the secondary extract of the stylosanthes guianensis, and filtering by using an ultrafiltration membrane to obtain the stylosanthes guianensis extract.
5. The method for preparing the feed additive for preventing and treating the digestive tract disease of the black goat as claimed in claim 1, wherein in the fourth step, the steps of decocting the weighed cattail for a plurality of times, combining the decoctions, and filtering to obtain the extract of the cattail comprise:
(1) Cleaning and airing the cattail grass, and cutting the aired cattail grass into sections to obtain a cattail grass section;
(2) Adding 10 times of deionized water into the obtained radix Ranunculi Ternati section, soaking for 1 hr, and decocting the soaked radix Ranunculi Ternati and soaking water in a Chinese medicinal decocting pot;
(3) Mixing the obtained decoctions, and filtering with a filter for multiple times to remove impurities to obtain radix Ranunculi Ternati extractive solution.
6. The method for preparing the feed additive for preventing and treating the digestive tract diseases of the black goats according to claim 5, wherein the decoction comprises the following steps: decocting for 3-4 hr for 2-3 times.
7. The method for preparing the feed additive for preventing and treating the digestive tract disease of the black goat as claimed in claim 1, wherein the step four, the wet milling, the enzymolysis, the leaching and the filtering of the weighed gynostemma pentaphylla to obtain the gynostemma pentaphylla extract comprises the following steps:
(1) Adding a certain amount of clear water into the gynostemma pentaphylla, and carrying out wet grinding by using a wet grinding device to obtain gynostemma pentaphylla slurry;
(2) Adding cellulase into the obtained herba Gynostemmatis slurry, and performing enzymolysis at normal temperature for 40-45min to obtain herba Gynostemmatis enzymolysis solution and herba Gynostemmatis residue;
(3) Adding a mixed solvent obtained by mixing propylene glycol and dichloromethane according to a ratio of 1;
(4) Mixing the Gynostemma pentaphylla enzymolysis liquid and the Gynostemma pentaphylla extracting solution, and filtering by using an ultrafiltration membrane to obtain the Gynostemma pentaphylla extracting solution.
8. The feed additive for preventing and treating the digestive tract disease of the black goat, which is prepared by the preparation method of the feed additive for preventing and treating the digestive tract disease of the black goat according to any one of claims 1 to 7, is characterized by comprising 20 to 25 parts of poplar leaves, 20 to 25 parts of stylosanthes guianensis, 10 to 15 parts of sarmentous sarmentosa, 10 to 15 parts of schizonepeta, 10 to 15 parts of cattail grass, 10 to 15 parts of pogostemon cablin, 5 to 8 parts of gynostemma pentaphylla, 5 to 8 parts of protein powder and 5 to 8 parts of microbial agent in parts by mass.
9. The feed additive for preventing and treating digestive tract diseases of the black goats according to claim 8, wherein the microbial agent is one or more of lactobacillus plantarum, bacillus subtilis, lactobacillus rhamnosus and pediococcus acidilactici.
10. A feed for preventing and treating digestive tract diseases of black goats, which is characterized in that the feed additive for preventing and treating digestive tract diseases of black goats, as claimed in any one of claims 8 to 9, is added.
CN202211202589.4A 2022-09-29 2022-09-29 Feed additive for preventing and treating digestive tract diseases of black goats and preparation method thereof Pending CN115606698A (en)

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CN112617028A (en) * 2020-12-16 2021-04-09 中国热带农业科学院热带作物品种资源研究所 Feed additive for feeding black goats and preparation method and application thereof
CN112617007A (en) * 2020-12-26 2021-04-09 广西大学 Selenium-rich high-vegetable-protein goat feed and preparation method and feeding method thereof
CN114041535A (en) * 2021-11-24 2022-02-15 石河子大学 Microbial preparation for improving intestinal environment of ruminants such as cattle and sheep and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104322868A (en) * 2014-10-17 2015-02-04 何占房 Goat breeding feed with functions of resisting diseases and putting on weight
CN106509398A (en) * 2015-09-10 2017-03-22 开县五通山羊股份合作社 Gastrointestinal disease preventing and curing Chinese herbal medicinal feed additive for black goats
CN107182922A (en) * 2017-06-09 2017-09-22 大化县满地畜牧有限公司 A kind of diseases prevention fattening cultivation method of black goat
CN107373112A (en) * 2017-07-29 2017-11-24 马鞍山市金农牧业有限公司 A kind of feed addictive for preventing and treating black goat disease of digestive tract
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CN114041535A (en) * 2021-11-24 2022-02-15 石河子大学 Microbial preparation for improving intestinal environment of ruminants such as cattle and sheep and preparation method thereof

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