CN112500232A - Fish protein leaf surface bio-organic fertilizer and application thereof - Google Patents

Fish protein leaf surface bio-organic fertilizer and application thereof Download PDF

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CN112500232A
CN112500232A CN202011566179.9A CN202011566179A CN112500232A CN 112500232 A CN112500232 A CN 112500232A CN 202011566179 A CN202011566179 A CN 202011566179A CN 112500232 A CN112500232 A CN 112500232A
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parts
bio
organic fertilizer
foliar
fish protein
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李凤兰
冯哲
冯艳忠
张世宏
李柱刚
陈勤
何流琴
***
袁强
王丽娟
吴建
杨秀梅
梁飞
田文会
常婷婷
杨劲松
姜雪峰
陈志刚
贺付蒙
赫兰保
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Northeast Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Soil Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a fish protein foliar bio-organic fertilizer and application thereof. The raw materials comprise the following components in parts by weight: 5-25 parts of cooked fish meat, 2-10 parts of monopotassium phosphate, 2-5 parts of urea, 5-60 parts of composite probiotics and 1000 parts of clear water 750-. When in use, the dosage is 0.5-1.0kg/hm2The dosage of the fertilizer is 200-fold and 300-fold diluted by clear water, and then the diluted solution is sprayed on the leaf surfaces of crops. The invention can convert the nutrient substances in the fish meat into nutrients required by the growth of crops, and the invention can promote the growth of the crops by spraying the nutrient substances on the leaves of the cropsThe crops grow and the crop yield is improved.

Description

Fish protein leaf surface bio-organic fertilizer and application thereof
Technical Field
The invention belongs to the technical field of fertilizers, and particularly relates to a fish protein foliar bio-organic fertilizer and application thereof.
Background
At present, the problems of gradually reduced cultivated land and slow agricultural yield increase in agriculture in China are increasingly prominent. Meanwhile, in order to improve the yield and the short-term economic benefit, farmers reduce the application of organic fertilizers and apply a large amount of fertilizers to farmlands. However, long-term unreasonable fertilization damages the structure and ecosystem of the soil, which leads to soil acidification, hardening, fertility reduction and pollution aggravation, and seriously affects the production of crops.
Therefore, the development of an efficient and environment-friendly organic fertilizer is of great significance to the sustainable development of rural economy.
Disclosure of Invention
The invention aims to provide a fish protein foliar bio-organic fertilizer and application thereof, which can convert nutrient substances in fish meat into nutrients required by crop growth.
On one hand, the invention provides a fish protein foliar bio-organic fertilizer which is prepared from raw materials consisting of cooked fish meat, monopotassium phosphate, urea, composite probiotics and clear water.
Preferably, the fish protein foliar bio-organic fertilizer is prepared by adding the cooked fish meat, the monopotassium phosphate, the urea and the composite probiotics into the clear water, uniformly mixing, controlling the fermentation temperature within the range of 25-33 ℃, and fermenting for more than 30 days.
Preferably, the raw materials comprise 5-25 parts of the cooked fish meat, 2-10 parts of the potassium dihydrogen phosphate, 2-5 parts of the urea, 5-60 parts of the composite probiotics and 1000 parts of the clean water 750-.
Preferably, the raw materials comprise, by weight, 5 parts of cooked fish meat, 7 parts of monopotassium phosphate, 3 parts of urea, 5 parts of composite probiotics and 750 parts of clean water.
Preferably, the raw materials consist of 13 parts of cooked fish meat, 2 parts of monopotassium phosphate, 4 parts of urea, 27 parts of composite probiotics and 840 parts of clear water by weight.
Preferably, the raw materials comprise 21 parts of cooked fish meat, 5 parts of monopotassium phosphate, 2 parts of urea, 47 parts of composite probiotics and 910 parts of clear water by weight.
Preferably, the raw materials comprise 25 parts of cooked fish meat, 10 parts of monopotassium phosphate, 5 parts of urea, 60 parts of composite probiotics and 1000 parts of clean water by weight.
Preferably, the complex probiotics comprise bacillus amyloliquefaciens, lactobacillus delbrueckii and saccharomyces cerevisiae.
Preferably, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 3.0 multiplied by 109cfu/mL, the bacterium content of the lactobacillus delbrueckii is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 108cfu/mL。
On the other hand, the invention provides the application of the fish protein foliar bio-organic fertilizer, the fish protein foliar bio-organic fertilizer is diluted by 200-300 times by clear water, and then the diluent is sprayed on the foliage of crops, wherein the spraying dosage of the fish protein foliar bio-organic fertilizer is 0.5-1kg/hm per time2。
The fish protein foliar bio-organic fertilizer can convert nutrient substances in fish meat into nutrients required by crop growth, and can promote crop growth and improve crop yield when being sprayed on the foliage of crops.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
According to the invention, cooked fish meat, monopotassium phosphate, urea, composite probiotics and clear water are proportioned according to a certain proportion, and then the fish protein foliar bio-organic fertilizer is obtained through fermentation. The fish protein foliar bio-organic fertilizer can convert nutrient substances in fish flesh into nutrients required by crop growth, and can promote crop growth and improve crop yield when being sprayed on crop foliage.
In a specific embodiment of the invention, the fish protein foliar bio-organic fertilizer is prepared from raw materials consisting of cooked fish meat, monopotassium phosphate, urea, composite probiotics and clear water. The specific preparation process comprises the following steps: step 1, preparing composite probiotics; step 2, preparing a mixed material; and 3, fermenting the mixed material.
In the step 1 of preparing the composite probiotics, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) bacterial liquid, Lactobacillus delbrueckii (Lactobacillus delbrueckii) bacterial liquid and Saccharomyces cerevisiae (Saccharomyces cerevisiae) bacterial liquid are selected, and then the three bacterial liquids are compounded according to the proportion relationship of the weight to obtain the composite probiotics, wherein 25-35 parts of the Bacillus amyloliquefaciens bacterial liquid, 30-45 parts of the Lactobacillus delbrueckii bacterial liquid and 20-35 parts of the Saccharomyces cerevisiae bacterial liquid are preferably selected. Wherein the bacterium content of the bacillus amyloliquefaciens bacterium liquid is more than or equal to 3.0 multiplied by 109cfu/mL, the bacterial content of the Lactobacillus delbrueckii bacterial liquid is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL. The three bacterial liquids can be prepared by self, and the bacillus amyloliquefaciens, the lactobacillus delbrueckii and the saccharomyces cerevisiae are respectively subjected to high-density fermentation culture in corresponding liquid culture media to obtain the bacterial liquids.
In the step 2 of preparing the mixed material, various raw material components are weighed according to a preset weight ratio, and then all the raw materials are uniformly mixed. The weight ratio of each component in the raw materials is respectively optimized as follows: 5-25 parts of cooked fish meat, 2-10 parts of monopotassium phosphate, 2-5 parts of urea, 5-60 parts of composite probiotics and 1000 parts of clear water 750-. The cooked fish meat can be prepared by itself, and the raw fish meat is subjected to a cooking treatment, particularly, the fish meat unsuitable for eating (such as deteriorated fish meat or low-value fish during transportation, storage or processing) is subjected to a cooking treatment, thereby obtaining the cooked fish meat.
In the step 3 of fermenting the mixed material, the initial pH of the mixed material is natural, the fermentation temperature is controlled within the range of 25-33 ℃, the mixture is subjected to facultative fermentation for more than 30 days, and the fermentation product is used as the fish protein foliar bio-organic fertilizer.
The invention also provides application of the fish protein foliar bio-organic fertilizer, wherein the fish protein foliar bio-organic fertilizer is diluted by 200-fold and 300-fold with clear water, and the diluent is sprayed on the foliage of crops according to the application method of the conventional foliar fertilizer for the crops, wherein the optimal spraying dosage of the fish protein foliar bio-organic fertilizer for each time is 0.5-1kg/hm2。
To help better understand the technical scheme of the present invention, the following examples are provided to illustrate the preparation process of the fish protein foliar bio-organic fertilizer of the present invention.
The first embodiment is as follows: preparation of fish protein leaf surface bio-organic fertilizer 1
The raw materials of the fish protein foliar bio-organic fertilizer comprise, by weight, 5 parts of cooked fish meat, 7 parts of monopotassium phosphate, 3 parts of urea, 5 parts of composite probiotics and 750 parts of clear water. The composite probiotics are formed by mixing bacillus amyloliquefaciens liquid, lactobacillus delbrueckii liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are respectively 25 parts by weight of bacillus amyloliquefaciens liquid, 41 parts by weight of lactobacillus delbrueckii liquid and 30 parts by weight of saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: the bacterial content of the bacillus amyloliquefaciens bacterial liquid is more than or equal to 3.0 multiplied by 109cfu/mL, the bacterial content of the Lactobacillus delbrueckii bacterial liquid is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL。
The fish protein foliar bio-organic fertilizer is prepared by the following steps.
Step 1, preparing the composite probiotics, and uniformly mixing the three bacteria liquids to obtain the composite probiotics.
And 2, preparing a mixed material, namely adding the cooked fish meat, the monopotassium phosphate, the urea, the clear water and the prepared composite probiotics together, stirring and mixing.
And 3, fermenting, wherein the initial pH of the mixed material is natural, the fermentation temperature is controlled within the range of 25-33 ℃, the fermentation is carried out for 35 days under facultative anaerobic condition, and the product after the fermentation is finished is used as the fish protein foliar bio-organic fertilizer 1.
Example two: preparation of fish protein leaf surface bio-organic fertilizer 2
The raw materials of the fish protein foliar bio-organic fertilizer comprise 13 parts of cooked fish meat, 2 parts of monopotassium phosphate, 4 parts of urea, 27 parts of composite probiotics and 840 parts of clear water by weight. The composite probiotics are formed by mixing bacillus amyloliquefaciens liquid, lactobacillus delbrueckii liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are 29 parts of the bacillus amyloliquefaciens liquid, 30 parts of the lactobacillus delbrueckii liquid and 35 parts of the saccharomyces cerevisiae liquid respectively. The bacterial contents of the three bacterial liquids are respectively as follows: the bacterial content of the bacillus amyloliquefaciens bacterial liquid is more than or equal to 3.0 multiplied by 109cfu/mL, the bacterial content of the Lactobacillus delbrueckii bacterial liquid is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL。
The fish protein foliar bio-organic fertilizer is prepared by the following steps.
Step 1, preparing the composite probiotics, and uniformly mixing the three bacteria liquids to obtain the composite probiotics.
And 2, preparing a mixed material, namely adding the cooked fish meat, the monopotassium phosphate, the urea, the clear water and the prepared composite probiotics together, stirring and mixing.
And 3, fermenting, wherein the initial pH of the mixed material is natural, the fermentation temperature is controlled within the range of 25-33 ℃, the fermentation is carried out for 33 days under facultative anaerobic condition, and the product after the fermentation is finished is used as the fish protein foliar bio-organic fertilizer 2.
Example three: preparation of fish protein leaf surface bio-organic fertilizer 3
The raw materials of the fish protein foliar bio-organic fertilizer comprise, by weight, 21 parts of cooked fish meat, 5 parts of monopotassium phosphate, 2 parts of urea, 47 parts of composite probiotics and 910 parts of clear water. The composite probiotics are formed by mixing bacillus amyloliquefaciens liquid, lactobacillus delbrueckii liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are 32 parts by weight of bacillus amyloliquefaciens liquid, 45 parts by weight of lactobacillus delbrueckii liquid and 26 parts by weight of saccharomyces cerevisiae liquid. Three kinds of bacteriaThe bacteria content of the liquid is respectively as follows: the bacterial content of the bacillus amyloliquefaciens bacterial liquid is more than or equal to 3.0 multiplied by 109cfu/mL, the bacterial content of the Lactobacillus delbrueckii bacterial liquid is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL。
The fish protein foliar bio-organic fertilizer is prepared by the following steps.
Step 1, preparing the composite probiotics, and uniformly mixing the three bacteria liquids to obtain the composite probiotics.
And 2, preparing a mixed material, namely adding the cooked fish meat, the monopotassium phosphate, the urea, the clear water and the prepared composite probiotics together, stirring and mixing.
And 3, fermenting, wherein the initial pH of the mixed material is natural, the fermentation temperature is controlled within the range of 25-33 ℃, the fermentation is carried out for 30 days under facultative anaerobic condition, and the product after the fermentation is finished is used as the fish protein foliar bio-organic fertilizer 3.
Example four: preparation of fish protein leaf surface bio-organic fertilizer 4
The raw materials of the fish protein foliar bio-organic fertilizer comprise, by weight, 25 parts of cooked fish meat, 10 parts of monopotassium phosphate, 5 parts of urea, 60 parts of composite probiotics and 1000 parts of clear water. The composite probiotics are formed by mixing bacillus amyloliquefaciens liquid, lactobacillus delbrueckii liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are 35 parts by weight of bacillus amyloliquefaciens liquid, 37 parts by weight of lactobacillus delbrueckii liquid and 20 parts by weight of saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: the bacterial content of the bacillus amyloliquefaciens bacterial liquid is more than or equal to 3.0 multiplied by 109cfu/mL, the bacterial content of the Lactobacillus delbrueckii bacterial liquid is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL。
The fish protein foliar bio-organic fertilizer is prepared by the following steps.
Step 1, preparing the composite probiotics, and uniformly mixing the three bacteria liquids to obtain the composite probiotics.
And 2, preparing a mixed material, namely adding the cooked fish meat, the monopotassium phosphate, the urea, the clear water and the prepared composite probiotics together, stirring and mixing.
And 3, fermenting, wherein the initial pH of the mixed material is natural, the fermentation temperature is controlled within the range of 25-33 ℃, the fermentation is carried out for 40 days under facultative anaerobic condition, and the product after the fermentation is finished is used as the fish protein foliar bio-organic fertilizer 4.
In order to help better understand the technical scheme of the invention, the following test examples of spraying the fish protein foliar bio-organic fertilizer of the invention on the foliar of different crops are provided for illustrating the application method of the invention and the effect thereof on the growth of the crops.
The first test example: influence of fish protein leaf surface bio-organic fertilizer on corn growth
And performing an application test of the fish protein foliar bio-organic fertilizer in the same corn field. The basic physical and chemical properties of the field soil are that the pH value is 6.6, the organic matter is 48.1g/kg, the total nitrogen is 2.33g/kg, the total phosphorus is 0.047%, the total potassium is 0.70%, the available phosphorus is 7.6mg/kg, and the quick-acting potassium is 77 mg/kg. The experiment designs 5 groups comprising 4 experiment groups and 1 control group, each group of experiment designs 3 experiment cells, each experiment cell area is 20m2All the test cells are randomly distributed, with a test period of 160 d.
Selecting Zhengdan 958, ditching with a seeder in late 4 th month, manually sowing with a rod with a ruler, wherein the row spacing is 0.6m, the plant spacing is 0.25m, and the planting density is 4500 plants/667 m2. Test group at 0.5L/hm2The dosage of the fish protein foliar bio-organic fertilizer is 200 times diluted by clear water, and the prepared fish protein foliar bio-organic fertilizer is sprayed in the small horn mouth period of corn. The control group was sprayed with equal amount of clear water. The corn is produced by adopting conventional management and harvested in the last 10 months.
The plant height of the corn in each district is counted in the large-horn mouth period, the tasseling and spinning period and the grouting period respectively, and the average plant height of each group is calculated. The results are shown in Table 1.
TABLE 1
Figure BDA0002861025640000061
As can be seen from the data in Table 1, the plant heights in the large bell mouth period, the emasculation and silking period and the grouting period of the four groups of corns applied with the fish protein foliar bio-organic fertilizer are all higher than those of the control group. Therefore, the prepared fish protein foliar bio-organic fertilizer 1-fish protein foliar bio-organic fertilizer 4 can obviously promote the growth of corns.
Before corn is harvested, randomly and continuously taking 5 plants from each cell for indoor seed test, and calculating the average ear length, the average ear thickness and the average ear row number of each group; after corn harvest, the yield of each cell was counted and the average cell yield per group was calculated. The results are shown in Table 2.
TABLE 2
Figure BDA0002861025640000062
As can be seen from the data in Table 2, the four groups of corns applied with the fish protein foliar bio-organic fertilizer are obviously higher than the control group in four properties of ear length, ear thickness, ear row number and cell yield. Therefore, the prepared fish protein foliar bio-organic fertilizer 1-fish protein foliar bio-organic fertilizer 4 can obviously promote the growth of corns and improve the yield of corns.
Test example two: influence of fish protein leaf surface bio-organic fertilizer on growth of winter wheat
And performing a fish protein foliar bio-organic fertilizer application test in the same winter wheat field. The basic physical and chemical properties of the field soil are that the pH value is 8.0, the organic matter is 8.95g/kg, the total nitrogen is 0.84g/kg, the available phosphorus is 34.6mg/kg, and the quick-acting potassium is 133 mg/kg. The total design of the experiment comprises 5 groups including 4 experiment groups and 1 control group, each group of experiments designs 3 experiment cells, and each experiment cell has an area of 50m2All the trial cells are randomly distributed, trial period 245 d.
Selecting Zhongmai 895, according to 10kg/667m in the last ten days of 10 months2The seeding amount of (2) and (3) seeding. The same conventional management is adopted in the growth process of the winter wheat, and the winter wheat is harvested in 6 middle months of the next year. Test group at 0.75L/hm2The dosage of the fish protein foliar bio-organic fertilizer is 250 times diluted by clear water, and the prepared fish protein foliar bio-organic fertilizer is respectively sprayed in the tillering stage, the flowering stage and the filling stage of winter wheat. ControlThe group was sprayed with equal amount of clear water.
Before wheat harvest, 3 random plots with the area of 1m are selected in each plot2The field of (2) was tested for effective spike number. And continuously taking 10 plants from each cell for indoor test, and counting the grain number per ear and the thousand grain weight. The average effective spike number, average spike grain number and average thousand grain weight of each group were calculated. After wheat is harvested, the yield of each cell is counted, and the average cell yield of each group is calculated. The results are shown in Table 3.
TABLE 3
Figure BDA0002861025640000071
As can be seen from the data in Table 3, the effective ear number, grain number, thousand grain weight and cell yield of the winter wheat applied with the fish protein foliar bio-organic fertilizer in the four groups are all obviously higher than those of the control group. Therefore, the prepared fish protein foliar bio-organic fertilizer 1-fish protein foliar bio-organic fertilizer 4 can obviously promote the growth of winter wheat and improve the yield of the winter wheat.
Test example three: influence of fish protein leaf surface bio-organic fertilizer on rice growth
And performing a fish protein foliar bio-organic fertilizer application test in the same paddy field. The basic physical and chemical properties of the field soil are that the pH value is 6.2, the organic matter is 42.1g/kg, the alkaline hydrolysis nitrogen is 15.1mg/kg, the quick-acting phosphorus is 24.8mg/kg, and the quick-acting potassium is 65 mg/kg. The total design of the experiment comprises 5 groups including 4 experiment groups and 1 control group, each group of experiments designs 3 experiment cells, and each experiment cell has an area of 25m2All the test cells are randomly distributed, and the test period is 150 d.
Selecting Fengyou 301, sowing and raising seedlings in the middle of 4 months, transplanting seedlings in the last 5 months, and planting 910 seedlings in each cell. The same conventional management is adopted in the rice growth process, and the rice is harvested in the last 10 months. Test group at 1.0L/hm2The dosage of the fertilizer is sprayed each time, the prepared fish protein foliar bio-organic fertilizer is respectively sprayed in the jointing-pulling heading stage, heading stage and grouting stage of rice after the fish protein foliar bio-organic fertilizer is diluted by 300 times by clear water. Control group spray-application of equal amount of liquidAnd (3) water.
Before rice harvest, continuously taking 15 plants in each cell for indoor seed test, and calculating the average plant height, average grain number per ear and average thousand grain weight of each group. After harvesting the rice, the yield of each cell is counted, and the average cell yield of each group is calculated. The results are shown in Table 4.
TABLE 4
Figure BDA0002861025640000081
As can be seen from the data in Table 4, the four groups of rice applied with the fish protein foliar bio-organic fertilizer are all obviously higher than the control group in four properties, namely plant height, grain number per ear, thousand grain weight and cell yield. Therefore, the prepared fish protein foliar bio-organic fertilizer 1-fish protein foliar bio-organic fertilizer 4 can obviously promote the growth of rice and improve the yield of rice.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.

Claims (10)

1. A fish protein foliar bio-organic fertilizer is prepared from raw materials consisting of cooked fish meat, monopotassium phosphate, urea, composite probiotics and clear water.
2. The fish protein foliar bio-organic fertilizer of claim 1, which is characterized in that: the fish protein foliar bio-organic fertilizer is prepared by adding the cooked fish meat, the monopotassium phosphate, the urea and the composite probiotics into the clear water, uniformly mixing, controlling the fermentation temperature within the range of 25-33 ℃, and fermenting for more than 30 days.
3. The fish protein foliar bio-organic fertilizer of claim 1, which is characterized in that: the raw materials comprise, by weight, 5-25 parts of cooked fish meat, 2-10 parts of monopotassium phosphate, 2-5 parts of urea, 5-60 parts of composite probiotics and 1000 parts of clean water 750-.
4. The fish protein foliar bio-organic fertilizer of claim 3, wherein: the raw materials comprise, by weight, 5 parts of cooked fish meat, 7 parts of monopotassium phosphate, 3 parts of urea, 5 parts of composite probiotics and 750 parts of clear water.
5. The fish protein foliar bio-organic fertilizer of claim 3, wherein: the raw materials comprise 13 parts of cooked fish meat, 2 parts of monopotassium phosphate, 4 parts of urea, 27 parts of composite probiotics and 840 parts of clear water by weight.
6. The fish protein foliar bio-organic fertilizer of claim 3, wherein: the raw materials comprise, by weight, 21 parts of cooked fish meat, 5 parts of monopotassium phosphate, 2 parts of urea, 47 parts of composite probiotics and 910 parts of clear water.
7. The fish protein foliar bio-organic fertilizer of claim 3, wherein: the raw materials comprise 25 parts of cooked fish meat, 10 parts of monopotassium phosphate, 5 parts of urea, 60 parts of composite probiotics and 1000 parts of clear water by weight.
8. The fish protein foliar bio-organic fertilizer of any one of claims 1 to 7 wherein: the composite probiotics comprise bacillus amyloliquefaciens, lactobacillus delbrueckii and saccharomyces cerevisiae.
9. The fish protein foliar bio-organic fertilizer of claim 8, wherein: the bacteria content of the bacillus amyloliquefaciens is more than or equal to 3.0 multiplied by 109cfu/mL, the bacterium content of the lactobacillus delbrueckii is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 108cfu/mL。
10. The application of the fish protein foliar bio-organic fertilizer as claimed in any one of claims 1 to 9, wherein: diluting the fish protein foliar bio-organic fertilizer by 200-300 times with clear water, and then spraying the diluent on the foliage of crops, wherein the spraying dosage of the fish protein foliar bio-organic fertilizer for each time is 0.5-1kg/hm2。
CN202011566179.9A 2020-12-25 2020-12-25 Fish protein leaf surface bio-organic fertilizer and application thereof Pending CN112500232A (en)

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CN112573956A (en) * 2020-12-25 2021-03-30 东北农业大学 Seed dressing agent for promoting crop rooting and application thereof
CN112794750A (en) * 2021-01-11 2021-05-14 东北农业大学 Foliar fertilizer for increasing potato yield and preparation method thereof
CN113233936A (en) * 2021-06-10 2021-08-10 东北农业大学 Preparation method of leaf fertilizer for promoting crop rooting
CN113277899A (en) * 2021-06-10 2021-08-20 东北农业大学 Preparation method of leaf fertilizer rich in selenium and amino acid
CN114885776A (en) * 2022-06-16 2022-08-12 北京四良科技有限公司 Method for improving quality of triticale through soil improvement

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CN108821867A (en) * 2018-07-25 2018-11-16 娄作富 The method for preparing biological liquid fertilizer using fish scale, fish guts and its cleaning sullage
CN112500224A (en) * 2020-12-05 2021-03-16 安丘市天赐生物肥料有限公司 Method for preparing water-soluble fertilizer by using fish protein hydrolysate

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CN102060600A (en) * 2010-10-11 2011-05-18 山东郁茏生物科技有限公司 Fish protein organic compound fertilizer and preparation method thereof
CN104557199A (en) * 2014-12-30 2015-04-29 湖南省土壤肥料研究所 Method for producing amino-acid biological liquid fertilizer by utilizing fish wastes
CN104557339A (en) * 2015-02-03 2015-04-29 舟山源丰海洋生物科技有限公司 Preparation method of soluble fish protein ammonia acid fertilizer
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112573956A (en) * 2020-12-25 2021-03-30 东北农业大学 Seed dressing agent for promoting crop rooting and application thereof
CN112794750A (en) * 2021-01-11 2021-05-14 东北农业大学 Foliar fertilizer for increasing potato yield and preparation method thereof
CN113233936A (en) * 2021-06-10 2021-08-10 东北农业大学 Preparation method of leaf fertilizer for promoting crop rooting
CN113277899A (en) * 2021-06-10 2021-08-20 东北农业大学 Preparation method of leaf fertilizer rich in selenium and amino acid
CN114885776A (en) * 2022-06-16 2022-08-12 北京四良科技有限公司 Method for improving quality of triticale through soil improvement

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