CN102329754B - Novel strain of streptomyces fradiae and application thereof - Google Patents
Novel strain of streptomyces fradiae and application thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.
Description
Technical field
The invention belongs to for soil and fertilizer field, be specifically related to a kind of agricultural microorganism preparation and application thereof.
Background technology
White agriculture refers to the industrial new agricultural of Microbial resources industrialization, is often referred to the application of microbial fermentation engineering in agricultural.Agriculture production needs soil that nutritive substance and the living environment of plant growth are provided.For many years, using of chemical fertilizer played keying action to getting agricultural profit.Yet, due to long-term application chemical fertilizer, soil beneficial microbe colony is damaged, fertilizer is under-supply, all kinds of nutrients ratio imbalances, cause farmland ecological environment, soil physical and chemical property and soil microflora to be subject to destruction in various degree, affected to a certain extent the quality of agricultural-food, the current soil resource present situation of China, in the urgent need to being that measure solves the problem that soil is destroyed gradually by people, is guaranteed agricultural planting level and crop products quality.In the nitrogen of some beneficial microorganisms in can fixed air, activating soil, be difficult to the phosphorus, potassium and other nutritive elements that utilize, improve soil nutrient deliverability and water conservation, fertilizer-preserving ability, the continuous production ability that improves land resources, can also strengthen disease-resistant crops, reduces pesticide dosage.
In ten large countermeasures of China's ecological environmental protection, clearly advocate and will promote " ecological agriculture "; preserve the ecological environment and agricultural land soil; promote the production of pollution-free healthy food, promote biological cycle in the ecosystem, reach and keep and enhancing soil fertility and biological activity object.The microbial preparation that the beneficial microorganism of take is core or fertilizer product can play improvement soil, increase fertility of land, promote the effects such as growth, be the preferred production means of high-quality Agricultural product without pollution.Along with the fast development of China's modern agriculture, particularly green organic agricultural cultivated area constantly expands, and specified microorganisms bacterium is dissolved into agriculture production, has become the hot issue that current government and enterprise pay close attention to.
Summary of the invention
First the present invention provides a kind of streptomyces fradiae (Streptomyces fradiae) new bacterial strain CLIMAX-VSF.
The present invention also provides the microbial inoculum that contains streptomyces fradiae CLIMAX-VSF.
The present invention also provides the seed treatment agent that contains streptomyces fradiae CLIMAX-VSF.
The present invention also provides the bacterial manure that contains streptomyces fradiae CLIMAX-VSF.
The present invention also provides the sprinkling irrigation that contains streptomyces fradiae CLIMAX-VSF agent.
Streptomyces fradiae CLIMAX-VSF provided by the invention is isolated from the soil of high-yield field, and this bacterial strain is disease-resistant, fixing nitrogen, dissolving phosphor and dissolving potassium and secretion growth hormone performance very significantly.Preservation is carried out at Yi China Committee for Culture Collection of Microorganisms common micro-organisms center, and preservation accession designation number is CGMCC No.5133, and preservation date is on August 12nd, 2011.
Researchist is relatively barren at soil but obtain rhizosphere soil in cotton soil that output is higher, in soil supension, add phenol number to drip and pass through to select culture medium culturing, get the bacterial classification of compound characteristics and check under the microscope, after proving single CLIMAX-VSF thalline, pass through repeatedly separated and a large amount of bacterial strains repeatability cultivation screenings, obtain CLIMAX-VSF bacterial strain.CLIMAX-VSF bacterial strain, by cultural characteristic, micro-morphology, physiological and biochemical property and hereditary feature, determines that this bacterial strain meets the feature of streptomyces fradiae.
Streptomyces fradiae CLIMAX-VSF of the present invention can be prepared into microbial inoculum, wherein, the viable count of streptomyces fradiae CLIMAX-VSF can be 5,000,000,000/gram more than, be preferably 10,000,000,000/gram.
The microbial inoculum that contains streptomyces fradiae CLIMAX-VSF of the present invention, can be prepared as follows:
1) prepare streptomyces fradiae CLIMAX-VSF seed liquor;
2) solid multiplication of streptomyces fradiae CLIMAX-VSF is cultivated: seed liquor step 1) is linked in solid multiplication substratum under the aseptic aerobic conditions of 30 ± 2 ℃ and cultivates 7～10 days; Described solid multiplication medium optimization is for containing 50～70 parts, wheat bran, 3～6 parts, brown sugar, cottonseed cake or 20～40 parts of soya-bean cakes, 1～3 part, magnesium sulfate, 1～3 part, sodium-chlor, 1～4 part, calcium carbonate, pH7.0～7.5;
3) by step 3) substratum after multiplication culture carries out Air drying, obtains the former bacteria agent of streptomyces fradiae CLIMAX-VSF.
The present invention also provides the preparation method containing streptomyces fradiae CLIMAX-VSF microbial inoculum, and it comprises:
1) prepare streptomyces fradiae CLIMAX-VSF seed liquor;
2) solid multiplication of streptomyces fradiae CLIMAX-VSF is cultivated: seed liquor step 1) is linked in solid multiplication substratum under the aseptic aerobic conditions of 30 ± 2 ℃ and cultivates 7～10 days; Described solid multiplication substratum contains 50～70 parts, wheat bran, 3～6 parts, brown sugar, cottonseed cake or 20～40 parts of soya-bean cakes, 1～3 part, magnesium sulfate, 1～3 part, sodium-chlor, 1～4 part, calcium carbonate, pH7.0～7.5;
3) by step 3) substratum after multiplication culture carries out Air drying, obtains the former bacteria agent of streptomyces fradiae CLIMAX-VSF.
Seed treatment agent provided by the invention, preferably contains the component of following weight part:
Streptomyces fradiae CLIMAX-VSF microbial inoculum 30-90 part;
Carrier 5-30 part;
Medical stone 5-20 part;
Agricultural rare-earth 0-40 part;
Zinc sulfate 0-10 part.
Wherein, described carrier is preferably the mixture of one or more arbitrary proportions in zeolite powder, wilkinite, Attapulgite.
Bacterial manure provided by the invention, preferably contains the component of following weight part:
Streptomyces fradiae CLIMAX-VSF microbial inoculum 0.2-30 part
Fertilizer 30-100 part
Humic acid powder 10-50 part
N-p-k element 0-50 part.
N-p-k element refers to that nitrogen element can be selected from urea, ammonium sulfate, ammonium chloride or its combination for can be used for the conventional azophoska raw material of agricultural; Phosphorus can be selected from monoammonium phosphate, diammonium phosphate, calcium superphosphate, potassium primary phosphate, ground phosphate rock or its combination; Potassium is optional from potassium sulfate, Repone K, salt of wormwood, saltpetre or its combination.
Sprinkling irrigation agent provided by the invention, preferably contains following weight part ground component:
50～400 parts of streptomyces fradiae CLIMAX-VSF filtered liquids
300～600 parts of aminoacids solutions
10～200 parts of potassium primary phosphates
10～50 parts of boric acid
10～60 parts of manganese sulfate monohydrates
10～50 parts of Zinc Sulphate Heptahydrates
20～150 parts, urea
0～50 part of agricultural rare-earth, wherein, streptomyces fradiae CLIMAX-VSF filtered liquid is to stir 1～8 hour after streptomyces fradiae CLIMAX-VSF microbial inoculum is mixed according to 1: 50～200 ratio with water, filters the filtrate of gained.
Wherein, aminoacids solution can add deionized water with soluble amino acid powder to be made, and also can directly buy aminoacids solution.At present above two kinds of raw materials are all available, as Baoding Wei Xiang derived food company limited.
Novel strain of streptomyces fradiae CLIMAX-VSF provided by the invention, has disease-resistant, fixed nitrogen phosphorus decomposing very significantly and secretion growth hormone performance.This bacterium can significantly improve percentage of germination, seedling growth speed in the situation that soil does not have additional nutrient, and significantly suppress seedling stage and vegetative period disease.Aspect the synergy that is mixed for fertilizer, demonstrate this bacterium and possess good activating soil nutrient effect and anti-multiple cultivation obstacle performance.In addition, this bacterium can be made into by certain technique the liquid product use of spraying, and further by the use three-dimensional of streptomyces fradiae, makes the outer probiotics that can apply this kind of ecological environment-friendly type of rhizosphere and root.
The present invention is by adopting liquid+solid combined fermentation method to carry out value added by manufacture, the mode of adsorbing into solid preparation with traditional liquid fermenting is compared, sporulation quantity that the method is carried out bacterial classification propagation is higher, meta-bolites amount is large, and the biological activity of its product is better, and preservation period is longer.
CLIAMX-VSF streptomyces fradiae is natural yeast culture, its application has bright prospects, for the present invention has developed it, for making seed treatment agent, fertilizer synergist, three kinds of application of sprinkling irrigation agent, feasible scheme is provided, and has obtained good practical application effect.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Unless otherwise noted, " part " described in the present invention refers to " weight part ".
The evaluation of embodiment 1 streptomyces fradiae CLIMAX-VSF
The morphological specificity of streptomyces fradiae CLIMAX-VSF of the present invention, sees the following form.
Physiological and biochemical property is as follows:
| Test subject | Result | Test subject | Result | Test subject | Result |
| Glucose | + | Glycerine | + | Sodium malate | + |
| N.F,USP MANNITOL | + | Lactose | + | Sunmorl N 60S | + |
| Saligenin | - | Semi-lactosi | + | Trisodium Citrate | - |
| Raffinose | - | Melizitose | + | Sodium succinate | - |
| Starch | + | L-arabinose | + | Sodium malonate | - |
| Sorbyl alcohol | - | Wood sugar | + | Sodium hippurate | - |
| Sorbose | - | Cellobiose | + | Sodium Propionate | - |
| Sucrose | - | Tetrahydroxybutane | + | Sodium acetate | + |
| Melibiose | + | Amygdaloside | + | Sodium tartrate | - |
| Seminose | + | Trehalose | + | Casein amino acid enzyme | + |
| Maltose | - | Ribose | + | Amylase | + |
| Synanthrin | - | Glycogen | - | Gelatine liquefication | - |
| Rhamnosyl | - | Inositol | - | Nitrate reduction | + |
| Fructose | + | Galactitol | + | ||
Measured the sequence of the 16sRNA of streptomyces fradiae CLIMAX-VSF, sequence is as shown in SEQ ID No.1.Through comparison, higher with the sequence homology of the 16sRNA of the streptomyces fradiae of other announcement.
By above-mentioned morphological specificity, physiological ecological feature and 16sRNA sequential analysis, can determine that being separated to is the new bacterial strain of streptomyces fradiae really.
The biological characteristics of embodiment 2 streptomyces fradiae CLIMAX-VSF
1. secrete growth hormone performance
1) first the freeze-drying pipe of preserving streptomyces fradiae CLIMAX-VSF is activated.Concrete grammar is to be first linked in the inclined-plane of Gause I substratum to cultivate 15 days at 30 ± 2 ℃; After the bacterium colony that secures good health, being accessed in PDA substratum 30 ℃ cultivates 5 days, then select grow healthy bacterial classification be linked into through sterilizing in 20 parts of sucrose, 5 parts, sodium-chlor, 20 parts of peptones, 5 parts, magnesium sulfate, 3 parts of potassium primary phosphates, 1000 parts of liquid nutrient mediums that form of water, and then 150r/min, 30 ± 2 ℃ cultivate 3 days, obtain streptomyces fradiae seed liquor.
2) solid multiplication is cultivated: streptomyces fradiae seed liquor is linked in 60 parts, wheat bran, 5 parts, brown sugar, 30 parts of cottonseed cakes, 1.5 parts, magnesium sulfate, 1.5 parts, sodium-chlor, 2 parts of solid culture mediums (pH7.5) through 120 ℃ of above high-temperature sterilizations that form of calcium carbonate, under the aseptic aerobic conditions of 30 ± 2 ℃, cultivate, solid multiplication to viable count be 1,000,000,000/gram time carry out growth hormone horizontal checkout), find that its secreted plant hormone level is higher, the results are shown in following table.
Growth hormone kind and the level of the secretion of table 1 streptomyces fradiae
| 1000000000/gram | |
| Plant hormones regulators,gibberellins GA3 | 103.37mg/kg |
| Indolylacetic acid IAA | 4.82mg/kg |
| Phytokinin CTK | 2.61mg/kg |
2. anti-microbial property
Through inhibition zone, test: by access CLIMAX-VSF bacterial classification and pathogenic bacteria in culture dish, test its white space diameter, this bacterium of the larger explanation of diameter range is stronger to the inhibition ability of pathogenic bacteria:
The anti-microbial activity of table 2 streptomyces fradiae CLIMAX-VSF
| Pathogenic bacteria | Bacteriostatic diameter (mm) |
| Root Rot of Wheat germ | 29 |
| Verticillium dahliae | 20.5 |
| Cotton-wilt fusarium | 20 |
| Colletotrichum gossypii | 27 |
| Cucumber phytophthora mycosis | 28 |
| Folium Mali pumilae zonate spot bacterium | 19 |
| Willow anthrax bacteria | 35.5 |
| Bacterial wilt of peanut bacterium | 27 |
| Sweet potato bacterial wilt bacterium | 33.5 |
| Watermelon anthrax bacteria | 39.5 |
3. separate nitrogen Decomposing phosphate activity
Through long-term field test, follow the tracks of the plant through streptomyces fradiae CLIMAX-VSF seed treatment and soil inoculation, at lean soil, not fertilising state, contrast, find that it has significantly by the effect of insoluble phosphorus dissolving in soil and the effect of air fixed nitrogen, it is compared and will increase production respectively 16.7% and 37.2% with contrasting blank, and it is blank that the supply that demonstrates nitrogen element and phosphoric is all obviously better than contrast.
The preparation of the former bacteria agent of embodiment 3 streptomyces fradiae CLIMAX-VSF
The present invention adopts liquid-solid combined ferment culture process to carry out the cultivation of streptomyces fradiae microbial inoculum, by first liquid multiplication culture, is linked into solid culture multiplication culture again, and formed microbial inoculum has very strong bioenzyme activity, and concrete steps comprise:
1) seed preparation and liquid multiplication culture: first the freeze-drying pipe of preserving streptomyces fradiae CLIMAX-VSF is activated.Concrete grammar is to be first linked in the inclined-plane of Gause I substratum to cultivate 15 days at 30 ± 2 ℃; After the bacterium colony that secures good health, being accessed in PDA substratum 30 ℃ cultivates 5 days, then select grow healthy bacterial classification be linked into through sterilizing in 20 parts of sucrose, 5 parts, sodium-chlor, 20 parts of peptones, 5 parts, magnesium sulfate, 3 parts of potassium primary phosphates, 1000 parts of liquid nutrient mediums that form of water, and then 150r/min, 30 ± 2 ℃ cultivate 3 days, obtain streptomyces fradiae seed liquor.
2) solid multiplication is cultivated: streptomyces fradiae seed liquor is linked in 60 parts, wheat bran, 5 parts, brown sugar, 30 parts of cottonseed cakes, 1.5 parts, magnesium sulfate, 1.5 parts, sodium-chlor, 2 parts of solid culture mediums (pH7.5) through 120 ℃ of above high-temperature sterilizations that form of calcium carbonate, under the aseptic aerobic conditions of 30 ± 2 ℃, cultivates 7 days.
3) drying treatment: solid multiplication is put in kiln after cultivating and carries out Air drying.It is 8,000,000,000/g that dried streptomyces fradiae detects living bacteria count through colony counting method, and it is preserved as the former bacteria preparation packing of streptomyces fradiae warehouse-in.
The seed treatment agent that embodiment 4 contains streptomyces fradiae CLIMAX-VSF
30 parts of the former bacteria preparations of streptomyces fradiae, 5 parts, carrier (zeolite powder), 20 parts of medical stones, 10 parts, the zinc sulfate of embodiment 3 preparations, above raw material fully mixes and get final product.
The seed treatment agent that embodiment 5 contains streptomyces fradiae CLIMAX-VSF
90 parts of the former bacteria preparations of streptomyces fradiae of embodiment 3 preparation, carrier (wilkinite: Attapulgite 1: 1) 30 parts, 5 parts of medical stones, 40 parts of agricultural rare-earths, above raw material fully mixes and get final product.
The seed treatment agent that embodiment 6 contains streptomyces fradiae CLIMAX-VSF
60 parts of the former bacteria preparations of streptomyces fradiae, 10 parts, carrier (wilkinite), 10 parts of medical stones, 20 parts of the agricultural rare-earths of embodiment 3 preparations, 5 parts, zinc sulfate, above raw material fully mixes and get final product.
The bacterial manure that embodiment 7 contains streptomyces fradiae CLIMAX-VSF
2 parts of the former bacteria preparations of streptomyces fradiae of embodiment 3 preparation, 100 parts of fertilizers, 10 parts of humic acid powder, urea by 10 parts, nitrogen, diammonium phosphate by 20 parts, phosphorus, Repone K by 20 parts, potassium, after above-mentioned raw materials is fully mixed, obtain the bacterial manure containing streptomyces fradiae CLIMAX-VSF.
Embodiment 8 is containing the bacterial manure of streptomyces fradiae CLIMAX-VSF
30 parts of the former bacteria preparations of streptomyces fradiae, 30 parts of fertilizers, 50 parts of the humic acid powder of embodiment 3 preparations, obtain the bacterial manure containing streptomyces fradiae CLIMAX-VSF after above-mentioned raw materials is fully mixed.
Embodiment 9 is containing the bacterial manure of streptomyces fradiae CLIMAX-VSF
15 parts of the former bacteria preparations of streptomyces fradiae of embodiment 3 preparation, 50 parts of fertilizers, 30 parts of humic acid powder, ammonium chloride by 10 parts, nitrogen, calcium superphosphate by 10 parts, phosphorus, potassium sulfate by 10 parts, potassium, after above-mentioned raw materials is fully mixed, obtain the bacterial manure containing streptomyces fradiae CLIMAX-VSF.
Embodiment 10 is containing the sprinkling irrigation agent of streptomyces fradiae CLIMAX-VSF
1) the former bacteria preparation of streptomyces fradiae CLIMAX-VSF of embodiment 3 was added to water according to 1: 50 and carry out batch type, stir 5 hours, stirring velocity is 60rpm, then after being filtered, obtain and be rich in the streptomyces fradiae filtered liquid of the compositions such as thalline and plurality of enzymes and growth hormone, then be formulated as follows formula:
2) first by 300 parts of aminoacids solutions (purchased from Baoding Wei Xiang derived food company limited), 200 parts of potassium primary phosphates, 10 parts of boric acid, 60 parts of manganese sulfate monohydrates, 10 parts of Zinc Sulphate Heptahydrates, 20 parts, urea, 0.001 part, tensio-active agent.Above composition is uniformly mixed 30 minutes at 60 ℃ in retort, treat that temperature is down to 25 ℃ and adds below 50 parts of streptomyces fradiae filtered liquids, then stirring at normal temperature is made sprinkling irrigation agent for 20 minutes.
Embodiment 11 is containing the sprinkling irrigation agent of streptomyces fradiae CLIMAX-VSF
1) the former bacteria preparation of streptomyces fradiae CLIMAX-VSF of embodiment 3 was added to water according to 1: 200 and carry out batch type, stir 8 hours, stirring velocity is 70rpm, then after being filtered, obtain and be rich in the streptomyces fradiae filtered liquid of the compositions such as thalline and plurality of enzymes and growth hormone, then be formulated as follows formula:
2) first by 600 parts of aminoacids solutions (purchased from Baoding Wei Xiang derived food company limited), 10 parts of potassium primary phosphates, 50 parts of boric acid, 10 parts of manganese sulfate monohydrates, 50 parts of Zinc Sulphate Heptahydrates, 150 parts, urea, 50 parts of agricultural rare-earths, 0.002 part, tensio-active agent.Above composition is uniformly mixed 60 minutes at 60 ℃ in retort, treat that temperature is down to 25 ℃ and adds below 400 parts of streptomyces fradiae filtered liquids, then stirring at normal temperature is made sprinkling irrigation agent for 5 minutes.
Embodiment 12 is containing the sprinkling irrigation agent of streptomyces fradiae CLIMAX-VSF
1) the former bacteria preparation of streptomyces fradiae CLIMAX-VSF of embodiment 3 was added to water according to 1: 100 and carry out batch type, stir 6 hours, stirring velocity is 60rpm, then after being filtered, obtain and be rich in the streptomyces fradiae filtered liquid of the compositions such as thalline and plurality of enzymes and growth hormone, then be formulated as follows formula:
2) first by aminoacids solution (purchased from 400 parts, Baoding Wei Xiang derived food company limited, 100 parts of potassium primary phosphates, 30 parts of boric acid, 30 parts of manganese sulfate monohydrates, 25 parts of Zinc Sulphate Heptahydrates, 80 parts, urea, 20 parts of agricultural rare-earths, 0.2 part, tensio-active agent.Above composition is uniformly mixed 40 minutes at 80 ℃ in retort, treat that temperature is down to 25 ℃ and adds below 200 parts of streptomyces fradiae filtered liquids, then stirring at normal temperature is made sprinkling irrigation agent for 10 minutes.
Test example 1 seed treatment agent
Before rice growing, with the seed treatment agent of embodiment 4～6, rice paddy seed is carried out to seed soaking.The seed rice of every mu of consumption is used 20 grams to add after 5 kg of water stir evenly and soak seed, and at about after planting 2 weeks, investigate discoverys, compare with Seed soaking, and rice shoot stalwartness, plant height, the increase of root length, grown in field is vigorous, setting percentage and thousand seed weight raising.Output on average increases by 10.5%, meanwhile, through field observation, also has the bakanae disease of control effect.
Test example 2 bacterial manure
In the base fertilizer phase, the bacterial manure of 80 kilograms of embodiment 7 of every mu of even ditch spread of grape.Identical with embodiment 7 formulas, but containing the contrast of the former bacteria preparation of streptomyces fradiae, do not compare, after using, can pluck in advance 5 days, while plucking, amount of increase in production reaches 18.1% simultaneously, and significantly improves commodity performance, and fruit look bright-coloured gloss, more than sugariness increases by 2 degree.Embodiment 7 and 8 bacterial manure also have similar effect.
Test example 3 sprinkling irrigation agent
After the slow seedling of tomato field planting and fast growing period respectively sprays and fill with the sprinkling irrigation agent of embodiment 10 in conjunction with root, wherein, each 200 milliliters spray according to water distribution in 1: 75, in conjunction with 5 kilograms of every mu of use according to filling with root at 1: 200 than water.Through tracking investigation, find, contrast and compare with clear water, after use, leaf viral disease and virus disease disease index decline 40-70%, average volume increase 15-20%, reach as high as 33%, and the stature of tomato is even, fruit look healthy, and single fruit representation work increases, and has good economic benefit.Embodiment 10 and 11 sprinkling irrigation agent also have similar effect.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. the new bacterial strain CLIMAX-VSF of streptomyces fradiae (Streptomyces fradiae), its preserving number is CGMCC No.5133.
2. the microbial inoculum that contains bacterial strain described in claim 1.
3. microbial inoculum according to claim 2, wherein the viable count of streptomyces fradiae CLIMAX-VSF be 5,000,000,000/gram more than.
4. according to the microbial inoculum described in claim 2 or 3, it is characterized in that, be prepared as follows:
1) prepare streptomyces fradiae CLIMAX-VSF seed liquor;
2) solid multiplication of streptomyces fradiae CLIMAX-VSF is cultivated: the seed liquor of step 1) be linked in solid multiplication substratum under the aseptic aerobic conditions of 30 ± 2 ℃, cultivate 7～10 days to viable count be 5,000,000,000/gram more than; Described solid multiplication substratum contains 50～70 parts, wheat bran, 3～6 parts, brown sugar, 20～40 parts of cottonseed cakes, 1～3 part, magnesium sulfate, 1～3 part, sodium-chlor, 1～4 part, calcium carbonate, pH7.0～7.5; Or described solid multiplication substratum contains 50～70 parts, wheat bran, 3～6 parts, brown sugar, 20～40 parts of soya-bean cakes, 1～3 part, magnesium sulfate, 1～3 part, sodium-chlor, 1～4 part, calcium carbonate, pH7.0～7.5;
3) substratum after step 3) multiplication culture is dried, obtains the former bacteria agent of streptomyces fradiae CLIMAX-VSF.
5. the seed treatment agent that contains bacterial strain described in claim 1, is characterized in that the component that contains following weight part:
Microbial inoculum 30-90 part claimed in claim 4;
Carrier 5-30 part;
Medical stone 5-20 part;
Agricultural rare-earth 0-40 part;
Zinc sulfate 0-10 part;
Described carrier is the composition of one or more arbitrary proportions in zeolite powder, wilkinite, Attapulgite.
6. the bacterial manure that contains bacterial strain described in claim 1, is characterized in that the component that contains following weight part:
Microbial inoculum 0.2-30 part claimed in claim 4;
Fertilizer 30-100 part;
Humic acid powder 10-50 part;
N-p-k element 0-50 part.
7. contain the sprinkling irrigation agent of bacterial strain described in claim 1, it is characterized in that the component that contains following weight part:
50～400 parts of streptomyces fradiae CLIMAX-VSF filtered liquids;
300～600 parts of aminoacids solutions;
10～200 parts of potassium primary phosphates;
10～50 parts of boric acid;
10～60 parts of manganese sulfate monohydrates;
10～50 parts of Zinc Sulphate Heptahydrates;
20～150 parts, urea;
0～50 part of agricultural rare-earth
0.001～0.2 part, tensio-active agent,
Wherein, streptomyces fradiae CLIMAX-VSF filtered liquid is the filtrate that microbial inoculum claimed in claim 4 and water are filtered to gained after according to the ratio mixing and stirring of 1:50～200.
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