CN1225236A - Pellet forpreventing nematode and prep. method thereof - Google Patents
Pellet forpreventing nematode and prep. method thereof Download PDFInfo
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- CN1225236A CN1225236A CN98114437A CN98114437A CN1225236A CN 1225236 A CN1225236 A CN 1225236A CN 98114437 A CN98114437 A CN 98114437A CN 98114437 A CN98114437 A CN 98114437A CN 1225236 A CN1225236 A CN 1225236A
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Abstract
The present invention relates to verticillium chlamydosporium isolated from soil as main ingredient in pellet preparation for preventing and treating parasitic nematode diseases of plants and production process thereof. The said preparation possesses the characteristics of long lasting, no pollution to environment, convenient to apply, and low cost.
Description
The invention belongs to the biopesticide of a kind of nematode, a kind of biologic product that utilizes natural enemy fungi control plant nematode and preparation method thereof of saying so in more detail.
Plant nematode diseases is various countries, the world today one of the most scabrous soil-borne diseases, multiple important nematodiasis such as soybean cyst nematode, various root knot nematode diseases have caused heavy losses to agricultural production, field crop is about 10% because of the nematode loss generally, vegetables and economic crops generally lose 20-30%, have in addition the total crop failure.Prevent and treat this class disease in the past and generally adopt chemical agent, go out and nemagon etc. as furans pellet, tears, but because these agricultural chemicals all are high-toxic pesticide, the environmental pollution that causes is very serious, therefore, some chemical nematocide is enacted a law by some countries in succession and is forbidden in recent years, causes chemical nematocide to move towards low ebb.Favored by the plant protection service person of countries in the world and utilize biologic product to prevent and treat nematode.The numerous and confused upright topic of a lot of countries is studied the nematode biocontrol microorganisms from Europe to America, as Pasteur puncture bacillus, Paecilomyces lilacinus etc.But going up spendable biocontrol fungicide as producing, is to count also seldom on our times.Existing biological prevention and control agent adopts seed dressing more or plants the form appearance of clothing agent, because various technical reasons, the bacterium amount that these preparations carry is limited, simultaneously, execute in the soil survival and grow surely that influenced by soil environment bigger, therefore, be difficult for forming colony, particularly the biocontrol microorganisms of obligatory parasitism nematode is all the more so, though the parasitic rate to nematode in soil is higher, because the time-to-live is shorter, the breeding difficulty, so, the general control poor effect.
The purpose of this invention is to provide biological and ecological methods to prevent plant disease, pests, and erosion granule of a kind of soil nematodes of high-efficiency prevention and control cheaply and preparation method thereof.
The objective of the invention is to realize by following content:
This nematode biological and ecological methods to prevent plant disease, pests, and erosion granule, wherein per 100 gram compositions are:
500-5000 chlamydospore of Verticillium chlamydosporium Verticillium chlamydospori-um
Water 10-15 gram
Trace element 0.05-0.2 gram
Carrier 84.9-89.9 gram.
Fungi that the present invention adopts is the Verticillium chlamydosporium from natural earth culture, and it is become former microbial inoculum through solid fermentation or liquid fermentation prepared in one of two ways.
The composition of the medium of the solid fermentation that is adopted is by weight percentage:
Jowar slag or maize pulp 70-80%
Water 20-30%
Above-mentioned solid-substrate fermentation temperature is: be 28-30 ℃ in first day to the 8th day, the 9th day was 18-25 ℃ to the 21 day.
The composition of the medium of the liquid fermentation that is adopted is by weight percentage:
Jowar is soaked juice 10-20%
Potassium nitrate 1-2%
Potassium dihydrogen phosphate 0.2-5%
Magnesium sulfate 0.2-0.3%
Ferric trichloride 0.01-0.03%
Sucrose 5-7%
Water 65-84%
Aforesaid liquid is cultivated keynote PH to 7-8 after 121 ℃ of sterilizations were used after 30 minutes, and fermentation temperature was 28-30 ℃ for beginning by the 48th hour, and 48 to 96 hours is 23-25 ℃, and 96 hours to 7 days is 16-22 ℃.
The production and processing technology flow process of this granule is seen accompanying drawing 1.
The Verticillium chlamydosporium of separating from natural soils of utilization of the present invention (Verticil-lium chlamydosporium) is the parasitical fungi of a kind of facultative parasitism torpescence nematode such as cyst or the female worm of root-knot nematode, therefore, when not having living nematode, this fungi also can carry out saprogenesis, easy long-term surviving and breed into certain colony in soil, simultaneously, the fungi that this preparation is used has chlamydospore, anti-transportation, storage, keep the active time longer, Plant nematode is had control efficiency steady in a long-term.
The price of preparation of the present invention and use cost only are the 30-50% of chemical agent, and said preparation is a biologic product simultaneously, and the lasting period is long, and is free from environmental pollution, to the person poultry safety, once apply during sowing, and saving of work and time, economic and social benefit is remarkable.
Embodiment 1
Take from right soil 50 grams, with soil dilute with water 5000-10000 doubly, at dull and stereotyped base (potato 200 grams, sucrose 20 grams, agar 18 grams of supporting of PDA, add water to 1000 milliliters) go up every ware and move into 20 microlitres, cultivation is after 3 days down in 25 ℃, and inspection obtains Verticillium chlamydosporium (Ve-rticillium chlamydosporium).Shift culture purified reserve down through 25 ℃ and do female microbial inoculum use.
Female microbial inoculum is carried out solid fermentation, temperature: first day to the 8th day is 28 ℃, and the 9th to the 21 day is 20 ℃.After fermenting 21 days as former microbial inoculum.
50 gram former microbial inoculums (every gram includes 20000 spores) add 50 gram zinc sulphate, and with 11.9 kilograms water and 88 kilograms dried poultrymanure mixing, the FS4 type particle pressing machine of producing through changzhou machinery plant is pressed into the particle of diameter 3mm.3 kilograms of consumptions of mu are broadcast into, control soybean Cyst nematode with seed, and effect reaches 60-70%, and its use cost is 30% of a furans pellet.
Embodiment 2
Take from right soil 50 grams, with 10000 times of soil dilute with waters, at dull and stereotyped base (potato 200 grams, sucrose 20 grams, agar 18 grams of supporting of PDA, add water to 1000 milliliters) go up every ware and move into 20 microlitres, cultivation is after 3 days down in 25 ℃, and inspection obtains Verticillium chlamydosporium (Verti-cillium chlamydosporium).Shift culture purified reserve down through 25 ℃ and do female microbial inoculum use.
Female microbial inoculum adopts solid fermentation, and temperature is: first day to the 8th day is 30 ℃, and the 9th to the 21 day is 22 ℃.After fermenting 21 days as former microbial inoculum.
50 gram former microbial inoculums (every gram includes 10000 spores) add 200 gram copper sulphate, and with 13.75 kilograms water and 86 kilograms dried pig manure mixing, the FS4 type particle pressing machine of producing through changzhou machinery plant is pressed into the particle of diameter 3mm.10 kilograms of consumptions of mu are broadcast into, control root-knot nematode with seed, and effect reaches 50-60%, and its use cost is the 50-60% of Aldicarb.
Embodiment 3
Take from right soil 50 grams, soil is diluted 10000 times with sterile water, at dull and stereotyped base (potato 200 grams, sucrose 20 grams, agar 18 grams of supporting of PDA, add water to 1000 milliliters) go up every ware and move into 20 microlitres, cultivation is after 3 days down in 25 ℃, and inspection obtains Verticillium chlamydosporium (Vert-icillium chlamydosporium).Shift culture purified reserve down through 25 ℃ and do female microbial inoculum use.
Female microbial inoculum adopts liquid fermentation, and the composition of medium is by weight percentage:
Jowar is soaked juice 12%
Potassium nitrate 1%
Potassium dihydrogen phosphate 0.4%
Magnesium sulfate 0.2%
Ferric trichloride 0.01%
Sucrose 5%
Distilled water 81.39%
Above-mentioned cultivation keynote PH to 7 day is after 121 ℃ of sterilizations were used in 30 minutes, and fermentation temperature is 28 ℃ for beginning by the 48th hour, and the 48th to the 96th hour is 23 ℃, and the 96th hour to 7 days is 16 ℃.After fermenting 7 days as former microbial inoculum.
100 milliliters of former microbial inoculums (every gram includes 40000 spores) add 100 gram boraxs, and with 10.8 kilograms water and 89 kilograms dried poultrymanure mixing, the FS4 type particle pressing machine of producing through changzhou machinery plant is pressed into the particle of diameter 3mm.10 kilograms of consumptions of mu are broadcast into, control root-knot nematode with seedling, and effect reaches 50-70%, and its use cost is the 50-60% that tears go out.
Embodiment 4
Take from right soil 50 grams, soil is diluted 10000 times with sterile water, support base (potato 200 grams, sucrose 20 grams, agar 18 grams at the PDA mean pole, add water to 1000 milliliters) go up every ware and move into 20 microlitres, cultivation is after 3 days down in 25 ℃, and inspection obtains Verticillium chlamydosporium (Vert-icillium chlamydosporium).Shift culture purified reserve down through 25 ℃ and do female microbial inoculum use.
Female microbial inoculum adopts liquid fermentation, and culture medium prescription is:
Jowar is soaked juice 20%
Potassium nitrate 2%
Potassium dihydrogen phosphate 5%
Magnesium sulfate 0.2%
Ferric trichloride 0.03%
Sucrose 5%
Distilled water 67.77%
Above-mentioned cultivation keynote PH to 8 day is after 121 ℃ of sterilizations were used in 30 minutes, and fermentation temperature is 30 ℃ for beginning by the 48th hour, and the 48th to the 96th hour is 25 ℃, and the 96th was 20 ℃ by 7 days frequently.After fermenting 7 days as former microbial inoculum.
100 milliliters of former microbial inoculums (every gram includes 30000 spores) add 150 gram sodium molybdates, with 12.75 kilograms water and 87 kilograms dried pig manure mixing, are pressed into the particle of diameter 3mm through particle pressing machine.3 kilograms of consumptions of mu are broadcast into, control soybean Cyst nematode with seed, and effect reaches 60-70%, and its use cost is 30% of a furans pellet.
Claims (6)
1, a kind of nematode biological and ecological methods to prevent plant disease, pests, and erosion granule is characterized in that per 100 gram compositions are as follows:
Verticillium chlamydosporium (Verticillium chlamydospori-um) 500-5000 chlamydospore
Water 10-15 gram
Trace element 0.05-0.2 gram
Carrier 84.9-89.9 gram.
2, a kind of nematode biological and ecological methods to prevent plant disease, pests, and erosion process for producing granula, it is characterized in that: to utilize the Verticillium chlamydosporium of from natural soils, separating (Verticillium chlamydospo-rium), the female microbial inoculum that adopts solid fermentation or solution fermentation and make is a main effect component, and it is in the particle of carrier that this mother's microbial inoculum is fixed in farmyard manure and trace element by the extruder grain method.
3, method according to claim 2 is characterized in that the composition of the medium that solid fermentation is used is by weight percentage:
Jowar slag or maize pulp 70-80%
Water 20-30%
Above-mentioned solid-substrate fermentation temperature is: be 28-30 ℃ in first day to the 8th day, the 9th day was 18-25 ℃ to the 21 day.
4, method according to claim 2 is characterized in that the composition of the medium of liquid fermentation is by weight percentage:
Jowar is soaked juice 10-20%
Potassium nitrate 1-2%
Potassium dihydrogen phosphate 0.2-5%
Magnesium sulfate 0.2-0.3%
Ferric trichloride 0.01-0.03%
Sucrose 5-7%
Water 65-84%
Aforesaid liquid is cultivated keynote PH to 7-8 after 121 ℃ of sterilizations were used after 30 minutes, and fermentation temperature was 28-30 ℃ for beginning by the 48th hour, and 48 to 96 hours is 23-25 ℃, and 96 hours was 16-22 ℃ by the 7th day.
5, nematode biological and ecological methods to prevent plant disease, pests, and erosion granule according to claim 1 is characterized in that said carrier is that farmyard manure is dried poultrymanure or dried pig manure.
6, nematode biological and ecological methods to prevent plant disease, pests, and erosion granule according to claim 1 is characterized in that said trace element is zinc or boron or copper or molybdenum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98114437A CN1098635C (en) | 1998-11-05 | 1998-11-05 | Pellet forpreventing nematode and prep. method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98114437A CN1098635C (en) | 1998-11-05 | 1998-11-05 | Pellet forpreventing nematode and prep. method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1225236A true CN1225236A (en) | 1999-08-11 |
| CN1098635C CN1098635C (en) | 2003-01-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98114437A Expired - Fee Related CN1098635C (en) | 1998-11-05 | 1998-11-05 | Pellet forpreventing nematode and prep. method thereof |
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| CN (1) | CN1098635C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102167641A (en) * | 2011-01-12 | 2011-08-31 | 重庆市众力生物工程有限公司 | 200 million live spore/g lichen Bacillus subtilis medical fertilizer granule and preparation method thereof |
| CN102657235A (en) * | 2012-05-10 | 2012-09-12 | 肇庆市真格生物科技有限公司 | Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof |
| CN105935064A (en) * | 2016-01-07 | 2016-09-14 | 金禾佳农（北京）生物技术有限公司 | Compound preparation for control of root knot nematode and preparation method thereof |
| CN106417385A (en) * | 2016-09-22 | 2017-02-22 | 江西天祥通用航空股份有限公司 | Hypocrea virens granules and preparation method thereof |
| CN107266158A (en) * | 2017-05-25 | 2017-10-20 | 江苏沃纳生物科技有限公司 | A kind of preparation of plant health agent for specially controlling root nematode and application process |
| CN110214793A (en) * | 2019-04-26 | 2019-09-10 | 云南微态源生物科技有限公司 | Thick wall cell verticil branch bacterium biological pesticide novel form and preparation method thereof |
| CN112616857A (en) * | 2020-11-05 | 2021-04-09 | 沈阳农业大学 | Liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07206619A (en) * | 1994-01-26 | 1995-08-08 | Nematetsuku:Kk | Formulation for controlling nematode parasitized in plant and method for controlling the same |
-
1998
- 1998-11-05 CN CN98114437A patent/CN1098635C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102167641A (en) * | 2011-01-12 | 2011-08-31 | 重庆市众力生物工程有限公司 | 200 million live spore/g lichen Bacillus subtilis medical fertilizer granule and preparation method thereof |
| CN102657235A (en) * | 2012-05-10 | 2012-09-12 | 肇庆市真格生物科技有限公司 | Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof |
| CN102657235B (en) * | 2012-05-10 | 2014-02-19 | 肇庆市真格生物科技有限公司 | Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof |
| CN105935064A (en) * | 2016-01-07 | 2016-09-14 | 金禾佳农（北京）生物技术有限公司 | Compound preparation for control of root knot nematode and preparation method thereof |
| CN106417385A (en) * | 2016-09-22 | 2017-02-22 | 江西天祥通用航空股份有限公司 | Hypocrea virens granules and preparation method thereof |
| CN107266158A (en) * | 2017-05-25 | 2017-10-20 | 江苏沃纳生物科技有限公司 | A kind of preparation of plant health agent for specially controlling root nematode and application process |
| CN110214793A (en) * | 2019-04-26 | 2019-09-10 | 云南微态源生物科技有限公司 | Thick wall cell verticil branch bacterium biological pesticide novel form and preparation method thereof |
| CN112616857A (en) * | 2020-11-05 | 2021-04-09 | 沈阳农业大学 | Liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1098635C (en) | 2003-01-15 |
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