CN109906934A - A method of promoting the formation of Porphyra yezoensis filamentous conchosporangia - Google Patents
A method of promoting the formation of Porphyra yezoensis filamentous conchosporangia Download PDFInfo
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Abstract
The invention belongs to marine organisms fields, more particularly to a kind of method for promoting Porphyra yezoensis filamentous conchosporangia to be formed using carbon dioxide or bicarbonate ion, Porphyra yezoensis filamentous is detected into conchosporangia formational situation by different gas concentration lwevels or bicarbonate ion concentration culture after three weeks, unexpected discovery of the present invention is with respect to high carbon dioxide concentration and high carbon acid hydrogen radical ionic conditions, and conchosporangia forms ratio highest under conditions of gas concentration lwevel is 1500 ppmoL or bicarbonate ion concentration is 4 mM.Operation of the present invention is simple, and method is effective, can shorten the time of nursery in production process, is conducive to synchronization raising breeding efficiency of collecting seedling, increases the economic benefit of seaweed industry.
Description
Technical field
The invention belongs to marine organisms fields, and in particular to a kind of that Porphyra yezoensis filamentous conchosporangia is promoted to be formed
Method.
Background technique
Porphyra yezoensis is a kind of important economical alga, is mainly cultivated in China North of Yangtze River area.Porphyra yezoensis industry
Scale is worldwide there are about more than 20 hundred million dollars, and more than 6000 tons of annual output, the output value accounts for 50% or so of China's seaweed total value.
The Porphyra yezoensis history of life is made of the thallus and microcosmic filamentous two eposides of macroscopic view, thallus, that is, membranaceous purple
Dish, filamentous, that is, so-called " seed ", filamentous culture are the bases of Porphyra yezoensis artificial breeding.Filamentous development experience is Filamentous
Algal filament, conchosporangia, conchospore are formed and the stages such as conchospore release.The production perithallium stage of development at sea completes,
The filamentous stage of development is to complete in nursery, therefore the filamentous stage of development is conducive to carry out manual control, can pass through control
Some stage for making its development promotes uniformly to collect seedling, and improves efficiency of collecting seedling.The formation of conchosporangia is the important link of nursery.
The primary condition of Porphyra yezoensis growth and development has been illustrated at present, but control conchosporangia formation there are efficacious prescriptions
Method obtains comprehensively solve not yet, and such as the inoculation of some nursery algal filaments is too late, numerous to promote nutrition algal filament to expand in process of production
Reach certain biomass, suitable control conchosporangia needed to be formed, however due to conchosporangia formed it is late will lead to it is subsequent
Stage of development lag, cannot collect seedling, the afloat growth cycle of seedling shortens, and is unfavorable for giving birth in time when at sea weather is suitable
It produces.
Therefore, in this case, algal filament biomass needs that suitable measure is taken to promote shell spore after reaching a certain level
The formation of ascus branch, the formation for controlling conchosporangia at present mainly passes through temperature control, control light, but these traditional methods are limited
How (such as weather, human cost) of system promotes conchosporangia to be formed in reasonable time and has become using effective method
To restrict the practical problem in Porphyra yezoensis cultivation production process.
It is existing that largely about the research and patent of Porphyra yezoensis sporinite, such as existing research, " environmental factor is to Porphyra yezoensis
The influence of sporinite growth and development " show four temperature, light intensity, sea water specific gravity and light dark period factors to the free silk of Porphyra yezoensis
The growth of shape body has a significant impact, and temperature and sea water specific gravity are an important factor for influencing its growth, specifically, research shows that sea
Water specific gravity is suitble to the radiation and attachment of conchospore between 1.020-1.025, and the influence degree diffused to conchospore that cools down is big
It is influenced in sea water specific gravity.
For another example Chinese patent CN201410056088.9 discloses a kind of half leaf porphyra breeding method, and this method includes following
Step: half leaf laver nutritive algal filament is obtained；Half leaf laver nutritive algal filament is cultivated in culture solution, until being formed has setting ratio
Sporangial branchlets filamentous, condition of culture is that temperature is 20-30 DEG C, light intensity 1000-2600lux, periodicity of illumination 10-
16h, culture solution sea water specific gravity are 1.026-1.031, the filamentous of the above-mentioned sporangial branchlets with setting ratio are placed on attached
On matrix, stimulation by running water completes the nursery of half leaf seaweed, to solve until forming the conchospore for meeting demand density of collecting seedling
The prior art of having determined can not double leaf seaweed carry out quickly, high efficiency seedling cultivating the problem of.
Existing research " application test of the triacontanol in Porphyra yezoensis nursery " shows that triacontanol can significantly improve
The forming amount of conchosporangia increases conchospore amount of diffusin, improves unit area seed harvesting amount.Specifically research shows that 0.016mg/L
Triacontanol conchosporangia forming amount improves 14.5% ~ 15.9%, and unit area output of seedling is compared and mentions 18.7% ~ 21.3%, shell spore
Sub- amount of diffusin is compared to increase by 20.8%.
Chen Cuifen etc. has studied different photoperiods, temperature, intensity of illumination to algal filament cutting induced synthesis conchosporangia
It influences, the results showed that in 25 DEG C and 57 μm of olm-2·s-1After CMC model 30d, short-day (8L:16d) is to conchosporangia
Induced synthesis effect is best, and conchosporangia formation rate is up to almost 100% after 92.5%, 50d.
Chen Meiqin etc. is with KNO3For nitrogen source culture filamentous, achieved in terms of promoting filamentous conchospore very well
Effect, but KNO3It is the raw material of industry, agrochemical is easy to get like that unlike, and price is also agricultural compared with other
Chemical fertilizer is height, consequently, it is desirable to which other lower-cost substances replace KNO3.Chen Ye etc. has studied different phosphorus sources to porphyra haitanensis shell
The influence that sporangial branchlets are formed, the results showed that regularly add the formation that microcosmic salt is conducive to porphyra haitanensis sporangial branchlets in right amount.
It yet there are no research achievement or patent that opposite high-carbon promotes Porphyra yezoensis conchosporangia to be formed.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for promoting Porphyra yezoensis conchosporangia to be formed.
To achieve the above object, the technical solution adopted by the present invention are as follows:
Two groups of Porphyra yezoensis filamentous are cultivated, wherein one group is passed through containing different CO2The air of concentration, the normal seawater training that sterilizes
It supports.Another group is added different HCO in artificial seawater culture solution3 -, the gas being passed through in incubation is 21% oxygen, 79% nitrogen
Gas.It is passed through CO in air2Concentration is respectively 40ppmoL, 400ppmoL, 1500ppmoL and 6000 ppmoL, wherein normal air
Middle CO2Content is 400ppmoL.HCO3 -Concentration is respectively 0mM, 0.02mM, 0.2mM, 2mM, 4mM, HCO3 -Concentration is 6 mM items
Occurring crystallization under part in culture solution can not continue to cultivate.Two groups of incubation times are three weeks, measure the photosynthetic activity of frond weekly
Variation, frond fresh weight, frond conchosporangia form ratio, the shadow that analysis different disposal forms filamentous conchosporangia
It rings, determines the effective ways for promoting conchosporangia to be formed.
Specific step is as follows:
(1) it controls carbon source concentration: PES(Provasoli ' s enriched Seawater Medium being added in natural sea-water,
PES) culture medium adjusts CO in air2Concentration；
(2) it controls carbon source concentration: preparing artificial seawater, weigh different amounts of NaHCO3Drug and the artificial seawater that preparation is added
In；
(3) start to cultivate: the Porphyra yezoensis filamentous for weighing no conchosporangia state is respectively placed in step (1) and step (2)
It is cultivated in different condition；
(4) measure: continuous culture three weeks uses weekly Dual-PAM Instrument measuring algal filament photosynthetic parameters, it is fresh to weigh algal filament
Weight calculates conchosporangia and forms ratio.
Wherein, CO in air is adjusted using saturation NaOH solution and gas mixed instrument in step (1)2Concentration；
Wherein, CO is passed through in step (1)2Concentration be respectively 40ppmoL, 400 ppmoL, 1500ppmoL and 6000 ppmoL；
Wherein, NaHCO is added in step (2)3After drug, HCO3 -Concentration is respectively 0 mM, 0.02 mM, 0.2 mM, 2 mM, 4
mM；
Wherein, Dual-PAM Instrument measuring algal filament photosynthetic parameters can be used in step (4).
The present invention is by Porphyra yezoensis filamentous in different CO2It is cultivated under concentration, culture is after three weeks, anti-under the conditions of different disposal
The parameter Fv/Fm difference for reflecting the potential maximum photosynthetic activity of algal filament is little (P > 0.05), in 40ppmoL CO2Under concentration conditions, shell
It is 0.60% that sporangial branchlets, which form ratio, and the more initial fresh weight of fresh weight increases 111.40%, in 400ppmoL CO2Under concentration conditions,
It is 7.13% that conchosporangia, which forms ratio, and more initial fresh weight increases 188%, and in 1500ppmoL CO2Under concentration conditions, shell
It is about 22.50% that sporangial branchlets, which form ratio, and more initial fresh weight increases 234%, and in 6000ppmoL CO2Under concentration conditions,
It is 3.33% that conchosporangia, which forms ratio, and more initial fresh weight increases 115%, it is seen then that 1500ppmoL CO2Concentration conditions lower casing
Sporangial branchlets form ratio and fresh weight rate of increase is apparently higher than other processing.
From this, lower CO2Higher CO2Concentration is unfavorable for the formation of Porphyra yezoensis filamentous conchosporangia,
1500ppmoL CO2Concentration is conducive to the formation of Porphyra yezoensis filamentous conchosporangia and fresh weight increases.
Porphyra yezoensis filamentous is placed in different HCO by the present invention3 -It is cultivated under concentration, culture is after three weeks, anti-in each processing
Reflect the parameter Fv/Fm difference of the potential maximum photosynthetic activity of algal filament less (P > 0.05).In HCO3 -Under the conditions of concentration is 0 mM, shell
It is 0.58% that sporangial branchlets, which form ratio, and more originally fresh weight reduces 27.27% to fresh weight, in HCO3 -Under the conditions of concentration is 0.02mM,
It is 23.02% that conchosporangia, which forms ratio, and more originally fresh weight increases 16.67% to fresh weight.In HCO3 -Concentration is 0.2mM condition
Under, it is 25.44% that conchosporangia, which forms ratio, and more originally fresh weight increases 14.31% to fresh weight, in HCO3 -Concentration is 2mM condition
Under, it is 40.08% that conchosporangia, which forms ratio, and more originally fresh weight increases 36.36% to fresh weight.In 4mM HCO3 -Concentration conditions
Under, it is 43.24% that conchosporangia, which forms ratio, and fresh weight increases 134.78%.
In view of this relatively high HCO3 -Concentration (4mM HCO3 -Concentration) be conducive to conchosporangia formation and fresh weight increasing
Long, different HCO3 -Concentration influences less (P > 0.05) filamentous photosynthetic activity.
The utility model has the advantages that
The present invention is by studying different CO2Concentration and different HCO3 -The influence that concentration forms conchosporangia finds two kinds of items
The formation of conchosporangia can more be promoted under part with respect to high carbon concentration, while filamentous fresh weight being promoted to increase, it is easy to operate,
Simple process, condition of culture is controllable, and method is effective, can shorten the time of nursery in production process, is conducive to synchronization of collecting seedling
Accelerate speed of collecting seedling, improves the economic benefit of seaweed industry.
(1) operation of the present invention process is simple, only need to be passed through CO in seedling growth greenhouse water body2HCO is added in gas3 -?；
(2) unexpected discovery of the present invention, in 1500ppmoL CO2Or 4mM HCO3 -Under concentration conditions, conchosporangia shape
Proportional highest.
(3) present invention saves time and cost, and incubation time is short, reduces the number for doing the wash shell, only can just make within three weeks
The conchosporangia of filamentous formation larger proportion.
Detailed description of the invention
Fig. 1 is difference CO in embodiment 12Photosynthetic activity variation diagram after concentration processing.
Fig. 2 is Porphyra yezoensis filamentous in embodiment 1 through different CO2Algal filament fresh weight variation diagram after concentration processing.
Fig. 3 is Porphyra yezoensis filamentous in embodiment 1 through different CO2Conchosporangia forms ratio chart after concentration processing.
Fig. 4 is Porphyra yezoensis filamentous in embodiment 2 through different HCO3 -Photosynthetic activity variation diagram after concentration processing.
Fig. 5 is Porphyra yezoensis filamentous in embodiment 2 through different HCO3 -Algal filament fresh weight variation diagram after concentration processing.
Fig. 6 is Porphyra yezoensis filamentous in embodiment 2 through different HCO3 -Conchosporangia forms ratio chart after concentration processing.
Specific embodiment
Present invention will be further explained below with reference to specific examples, it should be appreciated that these embodiments are merely to illustrate the present invention
Rather than limit the scope of the invention, in addition, it should also be understood that, after reading the contents of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within limited range of the present invention.
Embodiment 1
It is passed through CO2Concentration is that the air jet flow Porphyra yezoensis of 40ppmoL, 400ppmoL, 1500ppmoL and 6000ppmoL are Filamentous
Body:
1. adjusting CO using lye and gas mixed instrument using the method culture filamentous that seawater adds PES culture medium is boiled2Concentration
Filamentous culture solution is then passed to 40ppmoL, 400ppmoL, 1500ppmoL and 6000ppmoL to be cultivated；
2. 3 kinds of CO in step 12Cultivated filamentous three weeks under concentration conditions, using Dual-PAM measurement photosynthetic parameters,
Algal filament fresh weight, observation different disposal condition conchosporangia formational situation are weighed, in CO2Under the conditions of 40ppmoL, frond
Fv/Fm is 0.62, and algal filament fresh weight rate of increase is 111.4%, and it is 0.6% that conchosporangia, which forms ratio, in CO2For 400ppmoL
Under the conditions of, the Fv/Fm of frond is 0.64, and algal filament fresh weight rate of increase is 188%, and it is 7.13% that conchosporangia, which forms ratio,
CO2Under the conditions of concentration is 1500ppmoL, the Fv/Fm of frond is 0.61, and algal filament fresh weight rate of increase is 234%, conchosporangia
Formation ratio is 22.50%, and under 6000ppmoL CO2 concentration conditions, Fv/Fm 0.417, algal filament fresh weight rate of increase is
115%, it is 3.33% that conchosporangia, which forms ratio,.
Attached drawing 1 is different CO2Concentration handles the difference of Porphyra yezoensis filamentous photosynthetic activity, and maximum activity difference is not significant
P > 0.05, is marked with same letter a, and difference obviously uses different alphabetic flags；
Attached drawing 2 is different CO2Concentration culture Porphyra yezoensis filamentous fresh weight growth ratio significant difference P < 0.05, respectively with not
It is marked with letter a, b, c；
Attached drawing 3 is different CO2Concentration culture Porphyra yezoensis filamentous conchosporangia forms the significant P < 0.05 of proportional difference, point
It is not marked with different letter a, b, c；
As it can be seen that CO2Concentration in a certain range (40ppmoL-1500ppmoL), the CO of high concentration2Gas can promote streak purple
Dish filamentous fresh weight increases, while algal filament conchosporangia forms ratio and increases, wherein optimal concentration is 1500 ppmoL.
Embodiment 2
NaHCO is added in water body3, adjust HCO3 -Concentration is respectively 0 mM, 0.02 mM, 0.2 mM, 2 mM, 4 mM, is passed through
Nitrogen and oxygen culture filamentous, 6mM HCO3 -It is crystallized in nutrient solution under concentration conditions.
1, the method culture filamentous for adding PES culture medium using artificial seawater, is added different NaHCO3, adjust in culture medium
HCO3 -Concentration；
2. in above 5 kinds of HCO3 -It is cultivated filamentous three weeks under concentration conditions, measures photosynthetic parameters using Dual-PAM, claim
Algal filament fresh weight is measured, different disposal conchosporangia formational situation is observed, in HCO3 -Under the conditions of concentration is 0 mM, the Fv/Fm of frond
It is 0.58, algal filament fresh weight reduces 27.27%, and it is 0.58% that conchosporangia, which forms ratio,;In HCO3 -Concentration is 0.02 mM condition
Under, the Fv/Fm of frond is 0.59, and algal filament fresh weight rate of increase is 16.67%, and it is 23.02% that conchosporangia, which forms ratio,;?
HCO3 -Under the conditions of concentration is 0.2 mM, the Fv/Fm of frond is 0.58, and algal filament fresh weight rate of increase is 14.31%, conchosporangia
Formation ratio is 25.44%; HCO3 -Under the conditions of concentration is 2 mM, the Fv/Fm of frond is 0.57, and algal filament fresh weight rate of increase is
36.36, it is 40.08% that conchosporangia, which forms ratio,;HCO3 -Under the conditions of concentration is 4 mM, the Fv/Fm of frond is 0.56, algal filament
Fresh weight rate of increase is 134.78%, and it is 43.24% that conchosporangia, which forms ratio,.
Attached drawing 4 is different CO2Concentration culture Porphyra yezoensis filamentous conchosporangia forms the significant P < of proportional difference
0.05, it is marked respectively with different letter a, b, c；
Attached drawing 5 is different HCO3 -Concentration culture Porphyra yezoensis filamentous fresh weight growth ratio, it is not significant that same letter represents difference
(P > 0.05), different letters represent significant difference (P < 0.05)；
Attached drawing 6 is different HCO3 -Concentration culture Porphyra yezoensis filamentous conchosporangia forms ratio, and the significance of difference is used respectively
Difference letter a, b, c, d, e label, same letter represent difference not significantly (P > 0.05), and different letters represent significant difference (P
< 0.05).
The present invention utilizes different carbon source culture Porphyra yezoensis filamentous, and unexpected discovery is not simple gets over
High CO2Or HCO3 -Concentration more can promote Porphyra yezoensis filamentous conchosporangia to be formed, but have an optimal CO2Or
HCO3 -Concentration can get the optimum efficiency for promoting Porphyra yezoensis filamentous conchosporangia to be formed.
Operation of the present invention is easy, and simple process and low cost improves the breeding efficiency of Porphyra yezoensis.
The above are the descriptions to the embodiment of the present invention to keep this field special by the foregoing description of the disclosed embodiments
Industry technical staff can be realized or using the present invention.Various modifications to these embodiments carry out those skilled in the art
Saying will be apparent, and the general principles defined herein can be the case where not departing from the spirit or scope of the present invention
Under, it realizes in other embodiments.Therefore, the present invention will not be limited to the embodiments shown herein, but to accord with
Close the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. a kind of method for promoting Porphyra yezoensis filamentous conchosporangia to be formed, it is characterised in that: promoted using carbon dioxide
Porphyra yezoensis filamentous conchosporangia is formed.
2. a kind of method for promoting Porphyra yezoensis filamentous conchosporangia to be formed, is characterized in that: being promoted using bicarbonate ion
Form Porphyra yezoensis filamentous conchosporangia.
3. the method for promoting Porphyra yezoensis filamentous conchosporangia to be formed as described in claim 1, the carbon dioxide are dense
Degree range is 40 ppmoL-6000ppmoL.
4. as claimed in claim 2 promote Porphyra yezoensis filamentous conchosporangia formed method, the bicarbonate radical from
Sub- concentration range is 0-4mM.
5. the method for promoting Porphyra yezoensis filamentous conchosporangia to be formed as described in claim 1, wherein carbon dioxide is dense
Degree is 1500 ppmoL.
6. as claimed in claim 2 promote Porphyra yezoensis filamentous conchosporangia formed method, wherein bicarbonate radical from
Sub- concentration is 4mM.
7. such as the method that promotion Porphyra yezoensis filamentous conchosporangia claimed in claims 1-2 is formed, incubation time three
Week.
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| CN114586674A (en) * | 2022-04-21 | 2022-06-07 | 常熟理工学院 | Method for preserving laver filament |
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