CN114600808B - Method for cultivating nacre Bei Qinbei in pond to promote maturation - Google Patents

Method for cultivating nacre Bei Qinbei in pond to promote maturation Download PDF

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CN114600808B
CN114600808B CN202210424611.3A CN202210424611A CN114600808B CN 114600808 B CN114600808 B CN 114600808B CN 202210424611 A CN202210424611 A CN 202210424611A CN 114600808 B CN114600808 B CN 114600808B
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pond
bei
cultivation
nacre
qinbei
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CN114600808A (en
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廖永山
杨创业
邓岳文
王庆恒
梁飞龙
黄荣莲
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Guangdong Ocean University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G33/00Cultivation of seaweed or algae
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/40Culture of aquatic animals of annelids, e.g. lugworms or Eunice
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/59Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Cultivation Of Seaweed (AREA)

Abstract

The invention discloses a method for promoting maturation of a mother-of-pearl Bei Qinbei pond, which comprises the steps of pond selection, pond disinfection and water fertilization, wood row erection, and mother-of-pearl Bei Qin shellfish maturation promotion cultivation, wherein the environment of the algae phase of floating algae and the pond of the pond is regulated and kept relatively stable by cultivating Penaeus monodon, sand worm and Veraspergilli in the pond, so as to provide sufficient nutrition supply for the gonad development of the mother-of-pearl Bei Qinbei, and the environment of weak light and dissolved oxygen increase for the gonad development of the mother-of-pearl Bei Qinbei is provided on the wood row surface of the suspended-cultivated mother-of-pearl Bei Qinbei; the artificial maturation-promoting cultivation of the nacre Bei Qin shellfish by adopting the method provided by the invention has the advantages of high gonad development speed and high maturity of parent shellfish, and the larva for artificial breeding and hatching has the advantages of high growth speed, high survival rate and high metamorphosis rate.

Description

Method for cultivating nacre Bei Qinbei in pond to promote maturation
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a method for promoting maturation of nacre Bei Qinbei in a pond.
Background
The research of the Chinese pearl oyster cultivation technology starts in the 70 th century and has been 50 years so far, but the problem of low survival rate of young sea area cultivation exists. The survival rate of the pearl oyster seedlings cultured in the sea area to the adult pearl oyster suitable for the nuclear planting operation specification is lower than 5%, and the industrialized culture of the pearl oyster is seriously influenced.
The success and failure of the artificial shellfish breeding is mainly determined by proper parent shellfish, baits, water quality, larva breeding technology and other factors, wherein the proper parent shellfish has the greatest influence on the artificial breeding, and the breeding work can not be carried out without the mature parent shellfish. At present, no scale cultivation of the pinctada exists in China, and most of parent pinctada is collected from a natural sea area. The pinctada Bei Qin shellfish collected from the sea area usually has a small amount of gonads, and can be developed and matured only after being hung in the sea area, and parent pinctada can not be developed and matured and even die when the sea area is in poor environment, so that the normal operation of seedling raising is seriously affected.
Disclosure of Invention
Aiming at the problems that the gonad development maturity of the pinctada martensii collected in a sea area is low and seedling cultivation cannot be planned, the invention provides a method for promoting the maturation of the pinctada martensii Bei Qinbei in an enclosed pond, which can promote the gonad development maturation of the pinctada martensii Bei Qinbei faster and improve the utilization rate of parent pinctada martensii and the success rate of seedling cultivation.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a method for cultivating nacre Bei Qinbei in a pond for promoting maturation comprises the following steps:
(1) And (3) cultivation surrounding pond selection: selecting an enclosure pond with water inlet and outlet facilities as a cultivation place;
(2) Sterilizing and fertilizing water in an enclosure: sterilizing and fattening water treatment is carried out on the cultivation pond, then, the plaice fries are put in, sand worms are put in after 3-5 days, the penaeus monodon fries are put in after 2-3 days, artificial feed is not fed in 20-25 days after the penaeus monodon fries are put in, the penaeus monodon fries ingest wheel insects, water fleas, copepods and cladocera in water, and the artificial feed is fed in half of the feeding amount of the plaice fries and the penaeus monodon after 20-25 days;
(3) Erecting a cultivation wood row: erecting a clematis stem row in the middle of the pond;
(4) Promoting maturation and culturing the pinctada martensii: healthy nacre Bei Qin shellfish with strong pearl layer luster on the inner surface of the shell is selected, washed and cleanly moved to the wood raft erected in the step (3) to be hung and cultivated, and the feeding amount of the halibut and the penaeus monodon is adjusted or the water is changed to adjust the single-cell algae content of the cultivation pond to be within a set range.
Preferably, the cultivation pond in the step (1) has a size of 100-200 mu and a depth of 4.0-5.0 m.
Preferably, the length of the fries of the halibut put in the step (2) is 5-8cm, and the put amount is 200-300 fries per mu; the weight of the sandworm is 5-8 g per one, and 8-12 sandworm is put in per square meter; the young penaeus monodon is 1.2-1.5cm in body length and the adding amount is 2000-3000 tails per mu.
Preferably, the cultivation wood row in the step (3) consists of the stumps of the casuarina equisetifolia with the length of 7-9 meters and the wood strips crossing between the stumps, the distance between the stumps is 4-5 meters, the line spacing is 1.2-1.5 meters, the ulva is cultivated on the water surface below the wood row, the ulva can shield strong direct sunlight in daytime, oxygen is released through photosynthesis, water nutrient salts are consumed, and a good cultivation environment is provided for the gonad development of the nacre Bei Qinbei.
Preferably, the pearl layer on the inner surface of the shell of the healthy nacre Bei Qinbei in the step (4) is any one of malachite green, green and dark black, the cultivation density of the parent nacre in the cultivation pond is 700-1000 per mu, and the hanging cultivation water depth is 3.0-3.5 m.
Preferably, the concentration of the unicellular algae is set to be 2.5-3.5 ten thousand cells/mL.
Compared with the prior art, the invention has the beneficial effects that:
(1) The main technical measures of the parent shellfish artificial ripening cultivation of the pearl shellfish are to feed high-quality single-cell baits and increase the cultivation water temperature. However, the mass culture of high-quality unicellular algae baits requires special bait culture facility equipment and technicians, and requires large cost investment, and the technical and equipment requirements of the mass culture of unicellular algae are difficult to be met by a common seedling raising field. The invention forms a relatively stable ecological environment by collocating and breeding the halibut, the sand worms and the penaeus monodon in the surrounding pond. The method utilizes the herbivory of the halibut to ingest the macroalgae and the attached algae in the water body, utilizes the sandworm to ingest the attached diatom at the bottom layer of the water body, and utilizes the zooplankton edible of the shrimp larvae to ingest zooplankton such as rotifers, water fleas, copepods and cladocera in the water body to maintain the concentration of the floating algae in the culture pond relatively stable. The floating algae in the pond is high-quality food of the pinctada martensii, can promote the gonad development and maturation of the pinctada martensii Bei Qinbei, realize planned seedling raising and planned cultivation, fully utilize favorable conditions of sea areas and promote the growth and development of pinctada martensii seedlings. Under normal conditions, the cultivation of the nacre Bei Qinbei can be completed by properly changing water, and the operation is simple and easy.
(2) The pinctada martensii is attached to rock or coral reef for life, and inhabits water depth of 10-40 m, and ingests planktonic diatom and other micro-organisms. The environment illumination of the life of the pinctada is weaker, the water temperature is lower, and the bait biological abundance is low, so that the gonad development is slower and even can not mature. According to the invention, the ulva is cultivated on the water surface below the wood raft cultivated by the nacre Bei Qinbei, so that the ulva can shield strong direct sunlight in daytime, and the vast space around the wood raft can be strongly illuminated, so that mass propagation of planktonic algae can be promoted; and secondly, the ulva releases oxygen through photosynthesis and consumes nutrient salts in water, thereby providing a good cultivation environment for the gonad development of the nacre Bei Qinbei.
(3) Parent shellfish is hung on the cultivation wood raft, so that the sea area floating raft and the swing caused by the fluctuation of tide and the wave impact of the rope cultivation in the prior art are avoided. In addition, the seawater proportion, the temperature and the bait biological abundance of the cultivation pond are relatively stable, the attached organisms are fewer, the shellfish cleaning is not needed, and the mechanical damage and the harmful influence of the shellfish cleaning operation on the cleaning of the nacre Bei Qinbei are reduced.
(4) The invention selects peacocked green, green and dark black nacre Bei Zuoqin shell as the nacre on the inner surface of the shell, the cultured seedling grows into big shells for planting and culturing the pearl, and the cultured black pearl is mostly peacock green, green and dark black, and is the black pearl with highest selling price at present.
Therefore, the invention can provide an environment with weaker illumination, higher dissolved oxygen content and richer bait for the gonad development of the pinctada, the parent pinctada can develop and mature after culturing for 7-15 days, the pinctada Bei Qin is hung in an enclosure pond for culturing when the seedling is needed, and the parent pinctada after semen discharge and spawning can be matured again for artificial seedling culture after the maturing for 7-10 days, so that the utilization rate of the parent pinctada is improved.
Detailed Description
The present invention will be described in further detail by the following examples, which are only for the purpose of illustrating the present invention and are not to be construed as limiting the scope of the present invention.
Example 1
A method for cultivating nacre Bei Qinbei in a pond for promoting maturation comprises the following steps:
(1) And (3) cultivation surrounding pond selection: selecting a cultivation pond with water inlet and drainage facilities, an area of 100 mu and a water depth of 5 meters;
(2) Sterilizing and fertilizing water in an enclosure: performing pond disinfection and fertilizer water treatment according to a conventional method, putting 300 fish fries (total 3 ten thousand fish fries) of 5cm in length per mu, putting 12 sand worms with a weight of 5 g per square meter after 3 days, putting 3000 fish fries (total 30 ten thousand fish fries) of 1.2cm in length per mu of water body after 2 days, and putting the penaeus monodon fries without feeding artificial feed within 20 days, so that the penaeus monodon fries ingest wheel worms, water fleas, copepods and cladocera in the water body, and feeding the artificial feed according to half of the feeding amount of the penaeus monodon and penaeus monodon after 20 days;
(3) Erecting a cultivation wood row: erecting a wood row consisting of wood piles with the length of 9 meters and wood strips crossing the wood piles in the middle of the pond, wherein the pile distance is 5 meters and the row distance is 1.5 meters; ulva is cultivated on the water surface below the wood grate, strong direct sunlight can be shielded in the daytime by the Ulva, oxygen is released through photosynthesis of the Ulva, and nutrient salts in the water are consumed;
(4) Promoting maturation and culturing the pinctada martensii: selecting healthy nacre Bei Qin shell with strong luster and malachite green color on the inner surface of the shell, washing and cleaning, and moving to the wood row hanging cultivation erected in the step (3), wherein the parent pearl cultivation density is 1000 per mu, and the hanging cultivation water depth is 3.5 meters; the concentration of unicellular algae is 3.7 ten thousand cells/mL, the water is changed by 60cm, and the concentration of unicellular algae is reduced to 2.8 ten thousand cells/mL.
Example 2
A method for cultivating nacre Bei Qinbei in a pond for promoting maturation comprises the following steps:
(1) And (3) cultivation surrounding pond selection: selecting a cultivation pond with water inlet and outlet facilities, an area of 150 mu and a water depth of 4.5 m;
(2) Sterilizing and fertilizing water in an enclosure: performing pond disinfection and fertilizer water treatment according to a conventional method, putting 250 fish fries (total 3.75 ten thousand fish fries) of 6cm long halibut per mu, putting 10 sand worms of 6 g per square meter after 4 days, and putting 2500 fish fries (total 37.5 ten thousand fish fries) of 1.3cm long halibut per mu after 3 days; the method comprises the steps that artificial feed is not fed within 23 days after the penaeus monodon seedlings are fed, so that the penaeus monodon seedlings feed on rotifers, water fleas, copepods and cladocera in water, and the artificial feed is fed according to half of the feeding amount of the halibut and the penaeus monodon after 23 days;
(3) Erecting a cultivation wood row: erecting a wood row consisting of stumps with the length of 8 meters and battens crossing the stumps in the middle of the pond, wherein the pile distance is 4.5 meters and the row distance is 1.4 meters; ulva is cultivated on the water surface below the wood row, and can shield strong direct sunlight in the daytime, release oxygen through photosynthesis and consume nutrient salts in the water body;
(4) Promoting maturation and culturing the pinctada martensii: selecting healthy nacre Bei Qin shell with strong luster and green color on the inner surface of the shell, washing and cleaning, and moving to the wood row hanging cultivation erected in the step (3), wherein the parent shell cultivation density is 800 per mu, and the hanging cultivation water depth is 3.3 meters; the concentration of unicellular algae is 3.2 ten thousand cells/mL.
Example 3
A method for cultivating nacre Bei Qinbei in a pond for promoting maturation comprises the following steps:
(1) And (3) cultivation surrounding pond selection: selecting a cultivation pond with water inlet and outlet facilities and 200 mu area and 4 m water depth;
(2) Sterilizing and fertilizing water in an enclosure: performing pond disinfection and fertilizer water treatment according to a conventional method, putting 200 fish fries (total 4 ten thousand fish fries) of 8cm long halibut per mu, putting 8 sand worms of 8 g per square meter after 5 days, and putting 2000 fish fries (total 40 ten thousand fish fries) of 1.5cm long halibut per mu of water after 3 days; the method comprises the steps that artificial feed is not fed within 25 days after the penaeus monodon seedlings are fed, so that the penaeus monodon seedlings feed on rotifers, water fleas, copepods and cladocera in water, and the artificial feed is fed according to half of the feeding amount of the halibut and the penaeus monodon after 25 days;
(3) Erecting a cultivation wood row: erecting a wood row consisting of wood piles with the length of 7 meters and wood strips crossing the wood piles in the middle of the pond, wherein the pile distance is 4 meters, and the row distance is 1.2 meters; ulva is cultivated on the water surface below the wood row, and can shield strong direct sunlight in the daytime, release oxygen through photosynthesis and consume nutrient salts in the water body;
(4) Promoting maturation and culturing the pinctada martensii: selecting healthy nacre Bei Qin shell with strong luster and dark black color on the inner surface of the shell, washing and cleanly moving to the wood row hanging cultivation erected in the step (3), wherein the parent shell cultivation density is 700 per mu, and the hanging cultivation water depth is 3.0 meters; the concentration of single-cell algae is 2.3 ten thousand cells/mL, the feeding of the halibut and the penaeus monodon is changed from half to full feeding, and the concentration of single-cell algae is 2.7 ten thousand cells/mL after 4 days.
Comparative example 1
The selected nacre Bei Qinbei is washed cleanly and then put into a cage, and the pearl shell is moved to an excellent cultivation sea area with small stormy waves, smooth water flow and rich bait organisms, namely, the open sea of the Jiang city and the Qinghui county, and is hung and cultivated, the water depth is 3 meters, and the cultivation management is carried out according to the conventional cultivation method of the pearl shells.
The results of comparing the maturation-promoting effects of the nacre Bei Qin shellfish of examples 1-3 and comparative example 1 are shown in Table 1.
As can be seen from table 1, the maturation rate, maturation rate and spawning induction rate of the mother-of-pearl Bei Qinbei in-pond maturation-promoting cultivation are all significantly higher than those of the parent-shellfish in the sea area maturation-promoting cultivation, and the larva development rate of the parent-shellfish in-pond maturation-promoting cultivation is fast, and the larva floating time is significantly shortened than that of the parent-shellfish in the sea area.
TABLE 1 maturation-promoting and cultivating effects of nacre Bei Qin
Group of Ripening method Maturation time/day Rate of maturation/% Catalytic yield/% Larva planktonic time/day
Example 1 Surrounding pond 7 84.6 86.0 21
Example 2 Surrounding pond 8 88.2 85.5 23
Example 3 Surrounding pond 6 86.5 88.2 20
Comparative example 1 Sea area 12-24 54.5-58.0 58.5-72.0 26-35
In conclusion, the maturation-promoting cultivation method of the nacre Bei Qin shellfish can quickly promote gonad development maturation of parent shellfish, planned seedling raising is achieved, seedlings are bred according to a planned sea, the time suitable for seedling breeding in a sea area is fully utilized, seedling growth is promoted, and sufficient seedlings are provided for breaking through the technical bottleneck of low survival rate of the nacre sea area breeding. Secondly, the invention can also promote the maturation cultivation of parent shellfish after semen discharge and spawning, and then be used for seedling production, thereby improving the utilization rate of parent shellfish, and quickly obtaining mature parent shellfish after seedling failure to carry out seedling production again.
The method fully utilizes the precious and scarce resources of the nacre Bei Qinbei, can provide qualified mature parent nacre for the production of the nacre seedlings, provides sufficient seedlings for the artificial culture and proliferation and release of the nacre, and has very good application and popularization values.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (2)

1. The method for cultivating the nacre Bei Qinbei in the pond for promoting maturation is characterized by comprising the following steps of:
(1) Selecting an enclosure pond with water inlet and outlet facilities, the area of which is 100-200 mu and the depth of which is 4.0-5.0 meters as a cultivation place;
(2) Sterilizing and fattening water treatment are carried out in a cultivation pond, then, feeding the young fish of the halibut with the length of 5-8cm, wherein the feeding amount is 200-300 fish per mu, 8-12 sand worms with the weight of 5-8 g are fed after 3-5 days, the young fish of the halibut with the length of 1.2-1.5cm is fed after 2-3 days, the feeding amount is 2000-3000 fish per mu, and the feeding amount is 20-25 days, and then, artificial feed is fed according to half of the conventional feeding amount of the halibut and the halibut;
(3) Erecting a wood block of herba Ephedrae in the middle of the pond, wherein the wood block consists of wood blocks of 7-9 m long and wood strips crossing the wood blocks, the pile spacing is 4-5 m, the row spacing is 1.2-1.5 m, and the ulva is cultivated on the water surface below the wood block;
(4) Healthy nacre Bei Qin shellfish with strong pearl layer luster on the inner surface of the shell is selected, washed cleanly and then transferred to a wood raft erected in the step (3) for hanging and culturing, the cultivation density of parent shellfish in a cultivation pond is 700-1000 per mu, the hanging and culturing water depth is 3.0-3.5 m, the feeding amount of the halibut and the penaeus monodon is adjusted, and/or the single-cell algae content of the cultivation pond is adjusted to be 2.5-3.5 ten thousand cells/mL by changing water.
2. The method for promoting maturation of nacre Bei Qinbei in a pond according to claim 1, wherein the nacre shell inner surface in step (4) has a nacre shell color of either green or dark black.
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