CN103931482A - Porphyra haitanensis shell conchocelis ripening and seedling collecting method - Google Patents
Porphyra haitanensis shell conchocelis ripening and seedling collecting method Download PDFInfo
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Abstract
The invention aims at providing a porphyra haitanensis shell conchocelis ripening and seedling collecting method. The method comprises the following steps: (1) cultivating shell conchocelis before ripening, (2) ripening shell conchocelis, (3) synchronously diffusing conchospore and (4) collecting the conchospore. The porphyra haitanensis shell conchocelis ripening and seedling collecting method is simple to operate, shortens the transportation process, and can produce the spatfall porphyra haitanensis with good quality. Therefore, the production mode of improving synchronous diffusion of the porphyra haitanensis conchospore becomes an approach of relieving seedling cultivation and seedling collection of the porphyra haitanensis with good quality and reducing seedling cultivation cost. The porphyra haitanensis shell conchocelis ripening and seedling collecting method is simple and practicable and good in effect. Compared with the traditional technology, labor strength is reduced, working procedures are reduced, the quality of the porphyra haitanensis spore is good, and production efficiency is obvious.
Description
Technical field
The invention belongs to algae Biotechnology in Genetic Breeding field, a kind of method that is specifically related to Porphyra haitanensis shell conchocelis accelerating and collects seedling.
Background technology
Porphyra haitanensis has an edible tangleweed as a kind of, not only protein content high (generally in 34% left and right) but also vitamin content are abundant, also contain several amino acids and mineral nutrition, prevention of cardiac, cancer, constipation, reduction cholesterol are had to good function.
Nearly four more than ten years, because the raising of artificial breeding cultural technique is with universal, promote developing rapidly of Porphyra haitanensis aquaculture.Particularly utilize carpospore and free conchocelis inoculation shell, cultivate shell conchocelis, then accelerating, diffuses conchospore, obtains the technological approaches of seedling, has obvious advantage, makes the extensive popularization that obtained of this technology.The filamentous that is embodied in Porphyra haitanensis pierces shell pearl layer, can manual cleaning, and remove assorted algae, be not subject to the pollution of other assorted algaes.Publication number is that the patent of invention " a kind of indoor raising of seedling method of Porphyra haitanensis seedling " of CN102415329A discloses inoculation in nursery pond and has the shell string seedling management of free conchocelis, has promoted the method for cleaning; Publication number is that the patent of invention " ecological control method that a kind of Porphyra haitanensis is grown seedlings " of CN1561690 discloses the unicellular algas such as diatom that ingest by copepoda, and the assorted algae of cleaning in time, ensures that shell conchocelis has better growing conditions.
Porphyra haitanensis itself has and there is no single spore, limited diffuse conchospore number and can directly have influence on the required spore quality of breeding production and the feature of quantity, also causes the cost of growing seedlings to increase.Visible, there is good germplasm, might not there is the result of expectation.By gathering carpospore, cultivate shell conchocelis, if shell conchocelis has more healthy and stronger feature, can expect to obtain guaranteed conchospore quality and quantity, thallophytic growth after being conducive to.The cultivation quality of visible Porphyra haitanensis shell conchocelis will directly have influence on the growth of seedling.And individual upgrowth situation is the basis that forms colony's performance, the quality of colony is the principal security of whole output.
The incubation time of Porphyra haitanensis filamentous is long, at the beginning of annual 4 months, inoculate shell cultivation shell conchocelis, to the collection conchospore in September, the time that has 5 months is being carried out cultivation, the accelerating of filamentous and the period of diffusing spore, particularly high temperature period, water temperature will exceed 30 DEG C, and the high temperature of long term can not ensure to produce conchospore quality and quantity, and be unfavorable for later cultivation.It is only time of 4 months and carry out forming stage in sea area, for porphyra haitanensis seedling, really cultivate only with regard to about 15-30 days, the production major part of Porphyra haitanensis is by the Porphyra haitanensis shell conchocelis at indoor culture at present, reduce intensity of illumination by shortening light application time, promote the maturation of Porphyra haitanensis shell conchocelis conchospore.
And synchronously diffusing generally between spring tide flood season under Porphyra haitanensis nature completes, people are in order to impel conchospore synchronously to diffuse, often shell conchocelis is placed on marine greatly, by the dynamic stimulus of tidewater, realize and synchronously diffusing, often such action need 2-4 days.In order synchronously to diffuse, traditional method be need to carry out every day " shell conchocelis outbound---transport---sea area stimulates a night---transport---collecting seedling---change water " repeated work, synchronously to diffuse in order to promote purely, can not produce any benefit, and need to be from land to coastal waters, carrying from indoor to marine site, its labour intensity is large, shell easily occurs in transportation to be done, damage, affect the situation of diffusing of conchospore and the growth of seedling, diffuse and can not concentrate for conchospore, and the problem such as quality, the family of growing seedlings is often summed up as reason wherein the reason in weather or sea area.Therefore providing a kind of has great importance for the development of the artificial breeding technique that promotes Porphyra haitanensis in the synchronous method ripe, that synchronously diffuse conchospore of indoor promotion Porphyra haitanensis shell conchocelis.
Summary of the invention
A kind of method that technical problem to be solved by this invention is to provide Porphyra haitanensis shell conchocelis accelerating and collects seedling, be a kind of in the synchronous method ripe, that synchronously diffuse conchospore and adopt conchospore seedling of indoor promotion Porphyra haitanensis shell conchocelis, thereby make up the deficiencies in the prior art.
Method of the present invention, includes following step:
1) shell conchocelis before accelerating is cultivated
Utilize free conchocelis or healthy planting vegetables to carry out the cultivation of shell conchocelis, produce shell conchocelis, after cultivating, shell conchocelis is fully grown, be covered with whole shell;
2) accelerating of shell conchocelis
Cultivate after four months at shell conchocelis, add phosphate fertilizer and make the concentration of the P in seawater reach 5-15ppm; And the concentration ratio of P/N is become to 1:5-10; Shorten light application time to 8-10 hour, weaken intensity of illumination to 300-500Lx; Keep cultivating and within one month, make conchospore maturation;
3) conchospore synchronously diffuses
Ripe shell conchocelis is placed on and is collected seedling in container, and the seawater that is 3.5-5.0cm/s with flow velocity carries out stimulation by running water; After stimulation by running water, water temperature is reduced to 2-4 DEG C and promote that conchospore diffuses, wherein the content of the dissolved oxygen of seawater is 7-9ppm; And in seawater, add calcium chloride;
4) conchospore is collected seedling
In container, add seedling net collecting seedling, regulating the flow velocity of current is 1.2-3.0cm/S, and the intensity of regulating illumination is increased to 5000-8000Lx from 500Lx, after 2 hours, completes and collects seedling.
Wherein utilizing healthy planting vegetables to carry out the cultivation of shell conchocelis, is by the thallus with Porphyra haitanensis carpospore cyst, as planting vegetables, prepares carpospore liquid after drying in the shade, and this carpospore liquid is transplanted to shell;
Utilize free conchocelis to cultivate, filamentous, by smashing to pieces, is formed to the filamentous fragment of 0.15-0.35mm length, be transplanted in shell, produce shell conchocelis, and cultivate;
Described phosphate fertilizer is dipotassium hydrogen phosphate.
Wherein the interpolation concentration of calcium chloride is 0.2-0.5ppm.
Method simple operations of the present invention, reduce the process of transport, drop into economical, can produce good spatfall Porphyra haitanensis quality, therefore, the production model that this promotion Conchospores of Porphyra Hait Anensis synchronously diffuses, an approach of cultivation seedling cost is collected seedling, reduced to the cultivation seedling that becomes alleviation high-quality Porphyra haitanensis.Employing the method is simple and easy to do, and effect is better, compared with traditional technology, reduces labour intensity, alleviates working procedure, and the quality of Porphyra haitanensis spore is good, and productivity effect is obvious.
Embodiment
Technical problem to be solved by this invention is that filamentous is in indoor accelerating and the method for collecting seedling, and according to actual situation, adopt the method for different acceleratings according to varying environment, determine suitable stimulation by running water method, and in conjunction with suitable cooling measure, add certain density calcium chloride, promote that conchospore synchronously diffuses, realize former pond and adopt conchospore seedling, realize efficient accelerating and collect seedling technique.
Below in conjunction with specific embodiment, method of the present invention is described in detail.
1) shell conchocelis before accelerating is cultivated
Screening thallus stalwartness and that have obvious Porphyra haitanensis carpospore cyst, as planting vegetables, dries in the shade, and preparation carpospore liquid, is grafted directly to shell by this carpospore liquid;
Or utilize free conchocelis to cultivate, by filamentous by smashing to pieces, form the filamentous fragment of 0.15-0.35mm length, be transplanted in shell, produce shell conchocelis, and cultivate, the concentration of seawater N, P of growing seedlings is respectively 0.34ppm, 0.013ppm, the scope 15-28 of temperature DEG C, utilize consecutive days photograph, increase curtain at roof window and side window, with shading, make the illumination on shell conchocelis surface at 2000-3000Lx, start to cultivate at the beginning of April, shell conchocelis is fully grown, be covered with whole shell.
2) accelerating of shell conchocelis
Shell conchocelis is cultivated after nearly 3 months, at the bottom of shell conchocelis growth July, need to change the composition of nutritive salt, does not add or add a small amount of urea, reduces the concentration of N in water body.Add appropriate dipotassium hydrogen phosphate, make the concentration of the P in water body reach 5-15ppm.Increase the concentration ratio of P/N, from original 5-10:1, changed into 1:5-10.From afternoon 4-5 point to next day 8-9 point on nursery pond surface with the shading of shady deal cloth, shorten light application time to 8-10 hour, weaken intensity of illumination to 300-500Lx.By the roughly cultivation of month like this, can be down to ripe or approaching ripe.Now, the length of conchospore reaches 9-12 cell, occurs two points of cells, has shown that the conchospore in conchospore is tentatively ripe.
According to above-mentioned operation, the conchospore developing synchrotron in conchospore is better, and diffusing substantially of conchospore occurred in one day.And existing method filamentous growth is asynchronous, generally need 2 days, sometimes need 3-4 days.
3) diffusing of conchospore
In nursery pond, set up stimulation by running water method, ripe shell conchocelis is placed on to former pond, or filamentous shell is concentrated and is placed in mesh bag, be placed on the centre in nursery pond.Put into seawater, switch on the pump, cause current, flow velocity is 3.5-5.0cm/S.Open door and window, aeration-cooling; Changing current into stimulated after 2-4 hour, carried out cooling measure, and in nursery pond, cool-down method can be that ice cube is placed in the plastic sack of sealing, control temperature low 2-4 DEG C before relatively lowering the temperature,, promote that conchospore synchronously diffuses, now the content of the dissolved oxygen of seawater is 7-9ppm.The calcium chloride that adds 0.2-0.5ppm in water body further promotes diffusing of conchospore simultaneously, and diffusing of the conchospore of every piece of shell reaches 60-140 ten thousand.And the diffusing of conchospore of not adding every piece of shell of calcium chloride reaches 40-80 ten thousand.
4) conchospore is collected seedling
Realize former pond and adopt conchospore seedling, flow velocity is 1.2-3.0cm/S, light stimulation: the intensity of regulating illumination, is increased to 5000-8000Lx from 500Lx.There is diffusing peak Deng conchospore, according to the quantity of conchospore, put into lace curtaining to be collected seedling, after 2 hours, completed and collected seedling.Deng shifting out seedling net, change to new seawater, detect the online density of seedling, under microscope, to observe, the visual field of every 10 × 10 times, reaches 20-40 spore.
Often can not there is the peak of diffusing of conchospore in traditional mode of collecting seedling, can not reach the conchospore quantity of collecting seedling required.Its reason is because the time of diffusing peak appearance of conchospore be can not determine, therefore takes repeatedly the mode of collecting seedling to supplement and collects seedling, and need to repeatedly collect seedling with regard to the result of collecting seedling that has larger chance to produce.And method of the present invention only need to once be collected seedling and just can reach the needs of production.
Claims (5)
1. Porphyra haitanensis shell conchocelis accelerating and a method of collecting seedling, is characterized in that, described method includes following step:
1) shell conchocelis before accelerating is cultivated
Utilize free conchocelis or healthy planting vegetables to carry out the cultivation of shell conchocelis, produce shell conchocelis, after cultivating, shell conchocelis is fully grown, be covered with whole shell;
2) accelerating of shell conchocelis
Cultivate after four months at shell conchocelis, add phosphate fertilizer and make the concentration of the P element in seawater reach 5-15ppm; And the concentration ratio of P/N is become to 1:5-10; Shorten light application time to 8-10 hour, weaken intensity of illumination to 300-500Lx; Keep cultivating and within one month, make conchospore maturation;
3) conchospore synchronously diffuses
Ripe shell conchocelis is placed on and is collected seedling in container, and the seawater that is 3.5-5.0cm/s with flow velocity carries out stimulation by running water; After stimulation by running water, water temperature is reduced to 2-4 DEG C and promote that conchospore diffuses, wherein the content of the dissolved oxygen of seawater is 7-9ppm; And in seawater, add calcium chloride;
4) conchospore is collected seedling
In container, add seedling net collecting seedling, regulating the flow velocity of current is 1.2-3.0cm/S, and the intensity of regulating illumination is increased to 5000-8000Lx from 500Lx, after 2 hours, completes and collects seedling.
2. the method for claim 1, is characterized in that, described step 1) in utilize healthy planting vegetables to carry out the cultivation of shell conchocelis, by the thallus with Porphyra haitanensis carpospore cyst, as planting vegetables, after drying in the shade, prepare carpospore liquid, this carpospore liquid is transplanted in shell.
3. the method for claim 1, is characterized in that, described step 1) in utilize free conchocelis to cultivate, filamentous, by smashing to pieces, is formed to the filamentous fragment of 0.15-0.35mm length, be transplanted in shell, produce shell conchocelis, and cultivate.
4. the method for claim 1, is characterized in that, described step 2) in phosphate fertilizer be dipotassium hydrogen phosphate.
5. the method for claim 1, is characterized in that, described step 3) in the interpolation concentration of calcium chloride be 0.2-0.5ppm.
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| CN111066648A (en) * | 2019-12-30 | 2020-04-28 | 中国水产科学研究院黄海水产研究所 | Method for promoting formation of sporocyst branches of shells protonema of half-leaf laver |
| CN111201999A (en) * | 2020-02-18 | 2020-05-29 | 台州学院 | Method for promoting growth of free filaments and formation of sporangial branches of porphyra haitanensis |
| CN111727873A (en) * | 2020-08-03 | 2020-10-02 | 福建省平潭县水产良种实验有限公司 | Purple vegetable seedling oxygenation seedling raising pool and seedling raising and ripening method thereof |
| CN114982629A (en) * | 2022-06-16 | 2022-09-02 | 宁波大学 | Application of 1-aminocyclopropane carboxylic acid in promoting maturation of sporocyst branches of porphyra hyphomycete |
| CN114982629B (en) * | 2022-06-16 | 2024-03-26 | 宁波大学 | Application of 1-aminocyclopropane carboxylic acid in promoting maturation of aschersonia as laver filament |
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