CN1326449C - Method for preserving juvenile sporophyte of hijiki - Google Patents
Method for preserving juvenile sporophyte of hijiki Download PDFInfo
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- CN1326449C CN1326449C CNB2005100508236A CN200510050823A CN1326449C CN 1326449 C CN1326449 C CN 1326449C CN B2005100508236 A CNB2005100508236 A CN B2005100508236A CN 200510050823 A CN200510050823 A CN 200510050823A CN 1326449 C CN1326449 C CN 1326449C
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- setch
- harv
- water
- sargassum fusiforme
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The present invention discloses a method for preserving juvenile sporophyte seedlings of sargassum fusiforme(Harv.)Setch. The present invention is characterized in that seedlings are cultured in a kelp seedling culture pool whose water depth is from 30cm to 50cm and a prawn seedling culture pool whose water depth is from 80cm to 100cm; culture temperature is in the range of 5 DEG C to 20 DEG C, culture salinity is in the range of 20% to 35%, and culture light irradiation magnitude is from 5 mu EM-2S-1 to 50 mu EM-2S-1; flowing water stimulation is carried out by circulated water, and the water is changed in time. The present invention has the advantages that the juvenile sporophyte seedlings of sargassum fusiforme(Harv.)Setch can be stored for more than three months, and the survival rate of the seedlings can reach 58% to 76%; the method can reach productivity specifications, and meet the requirement of seedling culture; the present invention can store the juvenile sporophyte seedlings of the sargassum fusiforme(Harv.)Setch, and use the seedlings as experimental material at any time; individual sizes approximate to each other; the method is used for the research of the morphological change, the ecology, the toxicology and other aspects of algae; the stimulation of the flowing water is favorable to the normal growth of the juvenile sporophyte of the sargassum fusiforme(Harv.)Setch of certain density.
Description
Technical field
The present invention relates to a kind of preservation and cultivation of economical alga seedling, especially relate to a kind of store method of Sargassum fusiforme (Harv.) Setch sporophyte seedling.
Background technology
Sargassum fusiforme (Harv.) Setch Sargassum fusiforme (Harv) Setch is the perennial economical alga in warm temperate zone, in fields such as food, medicine, chemical industry very high using value is arranged.Control goitre, hypertension, colorectal cancer and elimination brain fag, promotion upgrowth and development of children all being had good food therapy value, be proposed as green health food, is the large-scale cultivation marine alga that important export is earned foreign exchange.The endurance that Sargassum fusiforme (Harv.) Setch is certain to coercing of environment such as red tide, the cultivation of carrying out Sargassum fusiforme (Harv.) Setch in the breed sea area can alleviate the degree of seawater eutrophication.The key of Sargassum fusiforme (Harv.) Setch cultivation is a seed, and the required seed of existing cultivation all is to derive from wild resource, and its main source is to gather gained from the marine site of west bank, the Pacific Ocean.Adopt the method for folder seedling cultivation can solve the seed problem of the cultivation of a part, the Sargassum fusiforme (Harv.) Setch of cultivation is cultivated maturation on the raft frame of sea area, obtain juvenile sporophyte, cultivate to seed size.But many problems are still arranged in this respect, and one of main problem is the preservation and the cultivation of sporophyte seedling.
Sargassum fusiforme (Harv.) Setch can be ripe in June, if adopt juvenile sporophyte in adherance, and the training seedling of directly plunging into the commercial sea, because the temperature in sea area maintains 25～30 ℃ to September, is unfavorable for the growth of Sargassum fusiforme (Harv.) Setch sporophyte seedling, and, the assorted algae in sea area, large-scale algaes such as Enteromorpha, sea lettuce are being covered sporophyte seedling; Some benthic diatoms in a large number attached to the surfaces of juvenile sporophyte, caused mass exchange not smooth, cause local fertilizer deficiency to lack light even on the surface, poor growth, even undergrowth present the phenomenon of depression and deformity, the decline of high-quality seedling rate, planting percent is not high.And severe sea conditions such as red tide that in the sea area, often occurs and typhoon, it is destructive that the sea of sporophyte seedling is cultivated, above-mentioned these situations all are to cause the unsettled major reason of present Sargassum fusiforme (Harv.) Setch juvenile sporophyte seedling.
The Sargassum fusiforme (Harv.) Setch sporophyte seedling of preserving is an approach that solves source of seedling.
Summary of the invention
Technical problem to be solved by this invention is the store method that a kind of Sargassum fusiforme (Harv.) Setch sporophyte seedling is provided at above-mentioned prior art present situation, guarantee that the Sargassum fusiforme (Harv.) Setch sporophyte seedling can be for a long time normally attached on the adherance, the Sargassum fusiforme (Harv.) Setch sporophyte seedling of preservation can be used as the source of seedling of sea area breed and the source of test material.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of store method of Sargassum fusiforme (Harv.) Setch sporophyte seedling, gather the Sargassum fusiforme (Harv.) Setch juvenile sporophyte, in the depth of water 30～50cm kelp seedling cultivation pond or in the depth of water 80～100cm prawn seed-rearing pond, carry out, its cultivation temperature scope is at 5～20 ℃, its cultivation salinity range is to be 20～35 ‰, and its cultivation light irradiance is 5～50 μ EM
-2S
-1, and carry out the stimulation by running water of cycle water and in time change water.
In the incubation of Sargassum fusiforme (Harv.) Setch sporophyte seedling, can add 0.1～1.0mg/m
3CaCl
2, can when changing water, add or directly adding.
Can add 1 time by above-mentioned concentration in per 10 days.
The speed of cycle water can be whole water body circulations in per 5～15 hours 1 time.
Can change water 1 time in per 10～30 days, the ratio of changing water is 1/3～1.
Compared with prior art, the invention has the advantages that can preserve the Sargassum fusiforme (Harv.) Setch sporophyte seedling surpassed more than 3 months, if collect seedling at the beginning of 6 months, after preservation Sargassum fusiforme (Harv.) Setch sporophyte seedling is aestivated, to October water temperature below 25 ℃, through taking exercise, the training seedling of plunging into the commercial sea can be avoided the bad breeding condition in sea area, planting percent can reach 58～76%, can reach the yielding ability specification, can satisfy folder seedling, breed before and after November, to next year 5, June results.If directly plunge into the commercial sea cultivation after collecting seedling, planting percent is generally 10%.
The present invention can preserve the Sargassum fusiforme (Harv.) Setch sporophyte seedling at any time as the material of test, and the size of its individuality is approaching, is used for the research of the aspects such as morphological change, ecology, toxicology of algae.
Add CaCl
2, concentration is at 0.1～1.0mg/m
3Help the growth and the firm attaching structure of formation of Sargassum fusiforme (Harv.) Setch juvenile sporophyte rhizoid, promote that the Sargassum fusiforme (Harv.) Setch sporophyte seedling can be normally attached on the adherance; The stimulation of flowing water helps the normal growth of the Sargassum fusiforme (Harv.) Setch juvenile sporophyte of certain density.
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
Embodiment one: gathering density is 4.85/cm
2The Sargassum fusiforme (Harv.) Setch juvenile sporophyte carries out the plane and cultivates in depth of water 30cm, area are 90 square metres kelp seedling cultivation pond, coolant-temperature gage is at 20～25 ℃; Transfer to the condition of culture of low temperature low light photograph after 30 days and cultivate down, by measures such as artificial hypothermia, shading, adjusting water levels, making culture condition is 5 ℃ of ocean temperatures, and by with salt crystal or fresh water, regulating salinity is 20 ‰, and control light irradiance is 5 μ EM
-2S
-1, and cultivate in conjunction with modes such as inflations, flowing water, per cultivation of carrying out flowing water in 1 day fully the water body circulation of cultivating 1 time, was changed water 1 time in per 10 days in per 5 hours, and the ratio of changing water is 1/3, when changing water, interpolation 0.1mg/m
3The CaCl of concentration
2, the size of Sargassum fusiforme (Harv.) Setch sporophyte seedling is at 1～2mm, and the density of spatfall reaches 5.8/cm
2, after one month, the size 15～35mm of sporophyte seedling, planting percent can reach 58～76%, satisfies the needs of growing seedlings.Such seedling can enter forming stage, and is general at the beginning of culturing 6 months on the raft frame in the sea area, just can gather in the crops.Can also be used to detect, analyze the toxicity intensity of red tide plankton.
Random sampling is measured, and takes off the seedling microscopy from adherance, can find to add CaCl2 Sargassum fusiforme (Harv.) Setch juvenile sporophyte spatfall rate influential of different concentration, the results are shown in Table 1.
Table 1 adds the CaCl of different concentration
2The influence of Sargassum fusiforme (Harv.) Setch juvenile sporophyte spatfall rate
| CaCl 2Concentration | 0 | 0.1 | 0.5 | 1.0 | 5.0 | 25 |
| Take off seedling rate % | 46.2 | 34.6 | 36.5 | 32.8 | 39.2 | 49.4 |
| Spatfall rate % | 53.8 | 65.4 | 63.5 | 67.2 | 60.8 | 50.6 |
In addition, cultivation temperature is higher than 25 ℃, is unfavorable for the normal formation of rhizoid, and the sporophyte seedling adhesive ability descends, and causes that easily juvenile sporophyte takes off seedling, and growth rate is too fast, and the condition of growth is difficult to control; And cultivation temperature is lower than 5 ℃, and the cost that then can cause cultivating increases.
Embodiment two: gathering density is 4.85/cm
2The Sargassum fusiforme (Harv.) Setch juvenile sporophyte carries out the plane and cultivates in depth of water 40cm, area are 95 square metres kelp seedling cultivation pond, coolant-temperature gage is at 20～25 ℃; After 30 days, transfer to the condition of culture of low temperature low light photograph and cultivate down, by measures such as artificial hypothermia, shading, adjusting water levels, making culture condition is 12 ℃ of ocean temperatures, and by with salt crystal or fresh water, regulating salinity is 25 ‰, and control light irradiance is 25 μ EM
-2S
-1, and cultivate in conjunction with modes such as inflations, flowing water, per cultivation of carrying out flowing water in 2 days fully the water body circulation of cultivating 1 time, was changed water 1 time in per 20 days in per 10 hours, and the ratio of changing water is 1/2, when changing water, interpolation 0.5mg/m
3The CaCl of concentration
2The size of Sargassum fusiforme (Harv.) Setch sporophyte seedling is at 1～2mm, and the density of spatfall reaches 5.8/cm
2, after one month, the size 15～35mm of sporophyte seedling, planting percent can reach 58～76%, satisfies the needs of growing seedlings.
Embodiment three: gathering density is 4.85/cm
2The Sargassum fusiforme (Harv.) Setch juvenile sporophyte carries out the plane and cultivates in depth of water 50cm, area are 105 square metres kelp seedling cultivation pond, coolant-temperature gage is at 20～25 ℃; After 30 days, transfer to the condition of culture of low temperature low light photograph and cultivate down, by measures such as artificial hypothermia, shading, adjusting water levels, making culture condition is 20 ℃ of ocean temperatures, and by with salt crystal or fresh water, regulating salinity is 35 ‰, and control light irradiance is 50 μ EM
-2S
-1, and cultivate in conjunction with modes such as inflations, flowing water, per cultivation of carrying out flowing water in 1.5 days fully the water body circulation of cultivating 1 time, was changed water 1 time in per 30 days in per 15 hours, changed the ratio 1 of water, when changing water, and interpolation 1.0mg/m
3The CaCl of concentration
2, the size of Sargassum fusiforme (Harv.) Setch sporophyte seedling is at 1～2mm, and the density of spatfall reaches 5.8/cm
2, the cultivation of plunging into the commercial sea mid-October, after one month, the size 15～35mm of sporophyte seedling, planting percent can reach 58～76%, satisfies the needs of growing seedlings.
Embodiment four: gathering density is 4.85/cm
2The Sargassum fusiforme (Harv.) Setch juvenile sporophyte is cultivated in depth of water 80cm, area are 95 square metres prawn seed-rearing pond, and coolant-temperature gage is at 20～25 ℃; Transfer to the condition of culture of low temperature low light photograph after 30 days and cultivate down, by measures such as artificial hypothermia, shading, adjusting water levels, making culture condition is 5 ℃ of ocean temperatures, and by with salt crystal or fresh water, regulating salinity is 30 ‰, and control light irradiance is 35 μ EM
-2S
-1, and cultivate in conjunction with modes such as inflations, flowing water, per cultivation of carrying out flowing water in 1 day fully the water body circulation of cultivating 1 time, was changed water 1 time in per 15 days in per 8 hours, and the ratio of changing water is 2/5, when changing water, interpolation 0.8mg/m
3The CaCl of concentration
2, the size of Sargassum fusiforme (Harv.) Setch sporophyte seedling is at 1～2mm, and the density of spatfall reaches 5.8/cm
2, after one month, the size 15～35mm of sporophyte seedling, planting percent can reach 58～76%, satisfies the needs of growing seedlings.
Claims (4)
1, a kind of store method of Sargassum fusiforme (Harv.) Setch sporophyte seedling, gather the Sargassum fusiforme (Harv.) Setch juvenile sporophyte, in the depth of water 30～50cm kelp seedling cultivation pond or in the depth of water 80～100cm prawn seed-rearing pond, carry out, its cultivation temperature scope is at 5～20 ℃, its cultivation salinity range is to be 20～35 ‰, and its cultivation light irradiance is 5～50 μ EM
-2S
-1, and carry out the stimulation by running water of cycle water and in time change water, it is characterized in that in the incubation of Sargassum fusiforme (Harv.) Setch sporophyte seedling, add 0.1～1.0mg/m
3CaCl
2, when changing water, add or directly adding.
2, the store method of a kind of Sargassum fusiforme (Harv.) Setch sporophyte seedling as claimed in claim 1 is characterized in that per 10 days by above-mentioned concentration interpolation 1 time.
3, the store method of a kind of Sargassum fusiforme (Harv.) Setch sporophyte seedling as claimed in claim 1, the speed that it is characterized in that cycle water are whole water body circulations in per 5～15 hours 1 time.
4, the store method of a kind of Sargassum fusiforme (Harv.) Setch sporophyte seedling as claimed in claim 1 is characterized in that changing water 1 time in per 10～30 days, and the ratio of changing water is 1/3～1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100508236A CN1326449C (en) | 2005-07-22 | 2005-07-22 | Method for preserving juvenile sporophyte of hijiki |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100508236A CN1326449C (en) | 2005-07-22 | 2005-07-22 | Method for preserving juvenile sporophyte of hijiki |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1736164A CN1736164A (en) | 2006-02-22 |
| CN1326449C true CN1326449C (en) | 2007-07-18 |
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|---|---|---|---|
| CNB2005100508236A Expired - Fee Related CN1326449C (en) | 2005-07-22 | 2005-07-22 | Method for preserving juvenile sporophyte of hijiki |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100508737C (en) * | 2007-04-01 | 2009-07-08 | 李生尧 | Artificial breeding method for sargassum fusiforme |
| CN102267604B (en) * | 2011-04-29 | 2013-03-13 | 浙江省海洋水产养殖研究所 | Method for transporting summering seedlings of Sargassum fusiform |
| CN104620969B (en) * | 2015-02-12 | 2017-01-11 | 宁波大学 | Rapid breeding method of Sargassum fusiforme |
| CN110249995A (en) * | 2019-07-30 | 2019-09-20 | 集美大学 | A method of long-term preservation pure laver line germ plasm resource at normal temperature |
-
2005
- 2005-07-22 CN CNB2005100508236A patent/CN1326449C/en not_active Expired - Fee Related
Non-Patent Citations (5)
| Title |
|---|
| 浙江海区羊栖菜人工育苗的生产性试验 吕明源，骆其君，中国水产，第9期 2000 * |
| 羊栖菜人工育苗的附着基筛选 骆其君，水产养殖，第3期 2001 * |
| 羊栖菜人工育苗的附着基筛选 骆其君，水产养殖，第3期 2001;羊栖菜幼孢子体苗海区培育的研究 骆其君，海洋湖沼通报，第3期 2001;羊栖菜幼孢子体室内的培育 骆其君，李维波，宁波大学学报（理工版），第15卷第4期 2002;浙江海区羊栖菜人工育苗的生产性试验 吕明源，骆其君，中国水产，第9期 2000 * |
| 羊栖菜幼孢子体室内的培育 骆其君，李维波，宁波大学学报（理工版），第15卷第4期 2002 * |
| 羊栖菜幼孢子体苗海区培育的研究 骆其君，海洋湖沼通报，第3期 2001 * |
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| CN1736164A (en) | 2006-02-22 |
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Granted publication date: 20070718 Termination date: 20100722 |